DE69606180T2 - Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt - Google Patents

Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt

Info

Publication number
DE69606180T2
DE69606180T2 DE69606180T DE69606180T DE69606180T2 DE 69606180 T2 DE69606180 T2 DE 69606180T2 DE 69606180 T DE69606180 T DE 69606180T DE 69606180 T DE69606180 T DE 69606180T DE 69606180 T2 DE69606180 T2 DE 69606180T2
Authority
DE
Germany
Prior art keywords
nucleic acid
probe
detecting
allows detection
detection once
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69606180T
Other languages
English (en)
Other versions
DE69606180D1 (de
Inventor
Marta Blumenfeld
Michel Bouillot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
Original Assignee
Serono Genetics Institute SA
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serono Genetics Institute SA, Genset SA filed Critical Serono Genetics Institute SA
Application granted granted Critical
Publication of DE69606180D1 publication Critical patent/DE69606180D1/de
Publication of DE69606180T2 publication Critical patent/DE69606180T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
DE69606180T 1995-07-31 1996-07-30 Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt Expired - Fee Related DE69606180T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9509290A FR2737502B1 (fr) 1995-07-31 1995-07-31 Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
PCT/FR1996/001201 WO1997005277A1 (fr) 1995-07-31 1996-07-30 Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection

Publications (2)

Publication Number Publication Date
DE69606180D1 DE69606180D1 (de) 2000-02-17
DE69606180T2 true DE69606180T2 (de) 2000-09-28

Family

ID=9481556

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69606180T Expired - Fee Related DE69606180T2 (de) 1995-07-31 1996-07-30 Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt

Country Status (9)

Country Link
US (1) US6228580B1 (de)
EP (1) EP0843738B1 (de)
JP (1) JP4050789B2 (de)
AT (1) ATE188745T1 (de)
AU (1) AU717454B2 (de)
CA (1) CA2228712A1 (de)
DE (1) DE69606180T2 (de)
FR (1) FR2737502B1 (de)
WO (1) WO1997005277A1 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19548680A1 (de) * 1995-12-23 1997-06-26 Boehringer Mannheim Gmbh Verfahren zum quantitativen Nachweis von spezifischen Nukleinsäuresequenzen
US20050069923A1 (en) * 1996-07-08 2005-03-31 Mullis Kary Banks Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus
US6083763A (en) 1996-12-31 2000-07-04 Genometrix Inc. Multiplexed molecular analysis apparatus and method
US20030049841A1 (en) * 1997-06-16 2003-03-13 Short Jay M. High throughput or capillary-based screening for a bioactivity or biomolecule
US20040241759A1 (en) * 1997-06-16 2004-12-02 Eileen Tozer High throughput screening of libraries
US20050070005A1 (en) * 1997-06-16 2005-03-31 Martin Keller High throughput or capillary-based screening for a bioactivity or biomolecule
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
WO2000037684A1 (en) * 1998-12-22 2000-06-29 Kris Richard M High throughput assay system using mass spectrometry
US6232066B1 (en) * 1997-12-19 2001-05-15 Neogen, Inc. High throughput assay system
US20030096232A1 (en) 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
AU2007231625B2 (en) * 1997-12-19 2011-04-07 Stephen Felder High Throughput Assay System
US6458533B1 (en) 1997-12-19 2002-10-01 High Throughput Genomics, Inc. High throughput assay system for monitoring ESTs
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
MXPA01005267A (es) * 1998-11-27 2002-04-24 Synaptics Uk Ltd Sensor de posicion.
US6849397B2 (en) * 1999-05-04 2005-02-01 Wisconsin Alumni Research Foundation Label-free detection of nucleic acids via surface plasmon resonance
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US6900013B1 (en) 2000-08-25 2005-05-31 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
AU2001285219A1 (en) * 2000-08-24 2002-03-04 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization
WO2002057491A2 (en) * 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
CA2446748C (en) * 2001-05-11 2011-03-08 University Of Ottawa Anxiolytic marcgraviaceae compositions containing betulinic acid, betulinic acid derivatives, and methods
US7083920B2 (en) * 2001-05-18 2006-08-01 Nagaoka & Co. Ltd. Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto
US7189509B2 (en) * 2001-08-16 2007-03-13 Zhifeng Shao Analysis of gene expression profiles using sequential hybridization
KR20030049802A (ko) * 2001-12-17 2003-06-25 권윤정 바이오 칩에서 프로브와 시료 핵산의 혼성화 여부를 효소반응으로 구별하는 방법
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
US20050019901A1 (en) * 2002-01-31 2005-01-27 Evgenia Matveeva Methods for synthesis of bio-active nanoparticles and nanocapsules for use in optical bio-disc assays and disc assembly including same
KR20020041344A (ko) * 2002-02-26 2002-06-01 김희태 멤브레인 크로마토그래프 기법을 활용하여핵산중합반응으로 증폭된 핵산을 검출하는 방법
KR100492393B1 (ko) * 2002-12-02 2005-05-30 굿젠 주식회사 멤브레인 크로마토그래프 기법에 의한 핵산 검출 방법 및이를 위한 킷트
US20040265883A1 (en) * 2003-06-27 2004-12-30 Biocept, Inc. mRNA expression analysis
US7294462B2 (en) * 2004-03-17 2007-11-13 Japan Software Management Co., Ltd. Method for detection of base sequence of interest
EP1580280A1 (de) * 2004-03-23 2005-09-28 Japan Software Management Co., Ltd. Methode zur Detektion von Basensequenzen.
US20060234253A1 (en) * 2004-10-08 2006-10-19 Sysmex Corporation Method for detecting a target substance by using a nucleic acid probe
US20060257902A1 (en) * 2005-03-25 2006-11-16 Ambion, Inc. Methods and compositions for depleting abundant RNA transcripts
WO2007050990A2 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
WO2007067907A1 (en) 2005-12-06 2007-06-14 Ambion, Inc. Reverse transcription primers and methods of design
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
WO2010062546A1 (en) * 2008-10-27 2010-06-03 Qiagen Gaithersburg Inc. Fast results hybrid capture assay on an automated platform
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
US9797000B2 (en) 2009-05-01 2017-10-24 Qiagen Gaithersburg Inc. Non-target amplification method for detection of RNA splice-forms in a sample
EP2478087B1 (de) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Zusammensetzungen und verfahren zur rückgewinnung von nukleinsäuren oder proteinen aus in zytologischen medien fixierten gewebeproben
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
US9689047B2 (en) 2010-01-29 2017-06-27 Qiagen Gaithersburg Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
AU2011255638B2 (en) 2010-05-19 2016-08-25 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
AU2012316129B2 (en) * 2011-09-28 2017-10-05 Htg Molecular Diagnostics, Inc. Methods of co-detecting mRNA and small non-coding RNA
MX2022001468A (es) 2019-08-09 2022-07-19 Nutcracker Therapeutics Inc Metodos y aparatos de fabricación para remover material de una composición terapeutica.

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5629158A (en) * 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
CA2116215C (en) * 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
CA2119701A1 (en) * 1993-04-16 1994-10-17 Colleen M. Nycz Nucleic acid assay procedure
EP0655091A1 (de) * 1993-05-06 1995-05-31 Baxter Diagnostics Inc. Nachweismethode des menschlichen papillomavirus
CA2125344A1 (en) * 1993-07-01 1995-01-02 Casey D. Morrow Encapsidated recombinant poliovirus nucleic acid and methods of making and using same
WO1995011316A1 (en) * 1993-10-22 1995-04-27 Amgen Inc. A NUCLEIC ACID DIAGNOSTIC METHOD USING RIBOPROBES, RNAse DIGESTION, AND MOLECULAR WEIGHT EXCLUSION CHROMATOGRAPHY
US5589329A (en) * 1993-11-15 1996-12-31 Ambion, Inc. Methods and compositions for detecting base pair mismatches
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5786203A (en) * 1994-06-14 1998-07-28 Neurocrine Biosciences, Inc. Isolated nucleic acid encoding corticotropin-releasing factor2 receptors

Also Published As

Publication number Publication date
EP0843738B1 (de) 2000-01-12
FR2737502A1 (fr) 1997-02-07
US6228580B1 (en) 2001-05-08
WO1997005277A1 (fr) 1997-02-13
EP0843738A1 (de) 1998-05-27
CA2228712A1 (fr) 1997-02-13
ATE188745T1 (de) 2000-01-15
AU6704596A (en) 1997-02-26
AU717454B2 (en) 2000-03-23
JP4050789B2 (ja) 2008-02-20
FR2737502B1 (fr) 1997-10-24
JPH11514850A (ja) 1999-12-21
DE69606180D1 (de) 2000-02-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee