DE69619959D1 - Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer - Google Patents

Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer

Info

Publication number
DE69619959D1
DE69619959D1 DE69619959T DE69619959T DE69619959D1 DE 69619959 D1 DE69619959 D1 DE 69619959D1 DE 69619959 T DE69619959 T DE 69619959T DE 69619959 T DE69619959 T DE 69619959T DE 69619959 D1 DE69619959 D1 DE 69619959D1
Authority
DE
Germany
Prior art keywords
target
pct
segment
dna
hybridizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69619959T
Other languages
English (en)
Other versions
DE69619959T2 (de
Inventor
Philippe Cleuziat
Bernard Mandrand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of DE69619959D1 publication Critical patent/DE69619959D1/de
Application granted granted Critical
Publication of DE69619959T2 publication Critical patent/DE69619959T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
DE69619959T 1995-07-24 1996-07-24 Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer Expired - Lifetime DE69619959T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9508945A FR2737223B1 (fr) 1995-07-24 1995-07-24 Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
PCT/FR1996/001166 WO1997004126A1 (fr) 1995-07-24 1996-07-24 Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres

Publications (2)

Publication Number Publication Date
DE69619959D1 true DE69619959D1 (de) 2002-04-25
DE69619959T2 DE69619959T2 (de) 2002-11-07

Family

ID=9481300

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69619959T Expired - Lifetime DE69619959T2 (de) 1995-07-24 1996-07-24 Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer

Country Status (7)

Country Link
US (1) US5824517A (de)
EP (1) EP0787209B1 (de)
AT (1) ATE214741T1 (de)
CA (1) CA2200627C (de)
DE (1) DE69619959T2 (de)
FR (1) FR2737223B1 (de)
WO (1) WO1997004126A1 (de)

Families Citing this family (136)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110097791A1 (en) * 1999-04-16 2011-04-28 Engelhardt Dean L Novel process, construct and conjugate for producing multiple nucleic acid copies
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
GB9806253D0 (en) * 1998-03-25 1998-05-20 Tepnel Medical Ltd Amplification of nucleic acids
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
DE19834948A1 (de) * 1998-08-03 2000-02-17 Monika Jecht Verfahren zur spezifischen Detektion von RNA
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6951722B2 (en) 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
CN100432237C (zh) * 1999-03-19 2008-11-12 宝生物工程株式会社 用于扩增核酸序列的方法
KR100682576B1 (ko) * 1999-03-19 2007-02-15 다카라 바이오 가부시키가이샤 핵산 서열 증폭 방법
US6232104B1 (en) * 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6677445B1 (en) 1999-08-27 2004-01-13 Chiron Corporation Chimeric antisense oligonucleotides and cell transfecting formulations thereof
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
CN102586228A (zh) * 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
EP1297179B1 (de) * 2000-05-12 2008-09-24 Caliper Life Sciences, Inc. Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) * 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
AU7878301A (en) * 2000-08-23 2002-03-04 Takara Shuzo Co Method of amplifying nucleic acid
AU8626801A (en) * 2000-09-19 2002-04-02 Takara Shuzo Co Method of forming complex
WO2002029117A2 (en) 2000-10-06 2002-04-11 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6858413B2 (en) * 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
EP1347060A4 (de) * 2000-12-26 2004-08-18 Takara Bio Inc Verfahren zum nachweis pathogener mikroorganismen
EP1359219A4 (de) * 2001-02-06 2004-09-22 Takara Bio Inc Amplifizierte nukleinsäuren und immobilisierte produkte davon
JP2005508135A (ja) * 2001-03-09 2005-03-31 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
ATE361996T1 (de) * 2001-03-09 2007-06-15 Nugen Technologies Inc Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
KR20040007620A (ko) * 2001-06-12 2004-01-24 다카라 바이오 가부시키가이샤 핵산 증폭 또는 검출용 시약의 안정화 방법 및 보존 방법
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US7056671B2 (en) * 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
US7045319B2 (en) 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
EP1488001B1 (de) * 2002-03-11 2008-08-20 Nugen Technologies, Inc. Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7393635B2 (en) * 2002-05-16 2008-07-01 Dna Polymerase Technology, Inc. Ribocloning: recombinant DNA construction using primers with ribo bases
US20040005614A1 (en) * 2002-05-17 2004-01-08 Nurith Kurn Methods for fragmentation, labeling and immobilization of nucleic acids
AU2003280603A1 (en) * 2002-10-29 2004-05-25 Kabushiki Kaisha Dnaform Method of amplifying nucleic acid
WO2004048596A2 (en) * 2002-11-21 2004-06-10 Epicentre Technologies Methods for using primers that encode one strand of a double-stranded promoter
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
AU2004230494A1 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
EP1641936B1 (de) * 2003-06-17 2010-08-04 Human Genetic Signatures PTY Ltd. Verfahren zur genomamplifikation
JP4714148B2 (ja) * 2003-09-04 2011-06-29 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 核酸検出アッセイ
KR20070012779A (ko) * 2003-10-29 2007-01-29 리보메드 바이오테그놀로지스 인코포레이티드 반복적 올리고뉴클레오티드 합성을 위한 조성물, 방법 및검출 기법
JP4249186B2 (ja) * 2003-12-10 2009-04-02 タカラバイオ株式会社 核酸の増幅方法
US20050136411A1 (en) * 2003-12-22 2005-06-23 Collins Patrick J. Methods and compositions for linear mRNA amplification from small RNA samples
WO2005063977A1 (ja) 2003-12-25 2005-07-14 Riken 核酸の増幅法およびこれを利用した変異核酸の検出法
EP1711591A4 (de) 2003-12-29 2010-04-28 Nugen Technologies Inc Verfahren zur analyse des methylierungsstatus von nukleinsäure und verfahren zur fragmentierung, markierung und immobilisierung von nukleinsäuren
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
DE102004034486B4 (de) * 2004-07-16 2007-08-23 Infineon Technologies Ag Verfahren zum Nachweis von Lumineszenzlicht aus einer porösen Trägerstruktur
ATE476439T1 (de) * 2004-09-10 2010-08-15 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
EP1828411B1 (de) * 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Verfahren zur vereinfachung mikrobieller nukleinsäuren durch chemische modifikation von cytosinen
WO2006066353A1 (en) * 2004-12-23 2006-06-29 Human Genetic Signatures Pty Ltd Detection of human papilloma virus
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
AU2006251866B2 (en) * 2005-05-26 2007-11-29 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US8343738B2 (en) * 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
KR100816419B1 (ko) * 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
WO2007067907A1 (en) 2005-12-06 2007-06-14 Ambion, Inc. Reverse transcription primers and methods of design
WO2007073737A1 (en) * 2005-12-29 2007-07-05 Exiqon A/S Detection of tissue origin of cancer
US9121056B2 (en) * 2006-04-28 2015-09-01 Igor Kutyavin Use of products of PCR amplification carrying elements of secondary structure to improve PCR-based nucleic acid detection
EP2021488B1 (de) 2006-04-28 2018-09-12 Igor Kutyavin Verwendung von basenmodifizierten deoxynukleosidtriphosphaten für verbesserten nukleinsäurenachweis
KR100785016B1 (ko) * 2006-05-22 2007-12-12 삼성전자주식회사 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
EP2038429B1 (de) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Verfahren zur fragmentierung und etikettierung von nukleinsäuren
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
EP2121983A2 (de) 2007-02-02 2009-11-25 Illumina Cambridge Limited Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
AU2008229628A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
US8153064B2 (en) 2007-03-22 2012-04-10 Doebler Ii Robert W Systems and devices for isothermal biochemical reactions and/or analysis
US8143006B2 (en) * 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
WO2009055732A1 (en) * 2007-10-26 2009-04-30 Rosetta Inpharmatics Llc Cdna synthesis using non-random primers
WO2009067743A1 (en) * 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
KR100957057B1 (ko) * 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
US20150079587A1 (en) * 2007-12-03 2015-03-19 Green Cross Medical Science Corp. Method for detecting nucleic acids by simultaneous isothermal amplification of nucleic acids and signal probe
US20110003700A1 (en) * 2007-12-20 2011-01-06 Human Genetic Signatures Pty Ltd. Elimination of contaminants associated with nucleic acid amplification
CA2711854C (en) 2008-01-09 2023-03-21 Keck Graduate Institute System, apparatus and method for material preparation and/or handling
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
EP2297346B1 (de) 2008-05-15 2015-04-15 Ribomed Biotechnologies, Inc. VERFAHREN UND REAGENZIEN FÜR DEN NACHWEIS VON CpG-METHYLIERUNG MIT EINEM METHYL-CpG-BINDENDEN PROTEIN (MBP)
JP5770085B2 (ja) 2008-06-30 2015-08-26 ライフ テクノロジーズ コーポレーション 粗製核酸サンプルからの直接増幅のための方法
WO2010009060A2 (en) * 2008-07-13 2010-01-21 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
WO2010030716A1 (en) * 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
AU2010225937B2 (en) 2009-03-15 2014-09-18 Ribomed Biotechnologies, Inc. Abscription based molecular detection
AU2010266034B2 (en) * 2009-06-26 2016-12-15 Claremont Biosolutions Llc Capture and elution of bio-analytes via beads that are used to disrupt specimens
WO2011037802A2 (en) 2009-09-28 2011-03-31 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
KR101317709B1 (ko) * 2010-05-14 2013-10-15 (주)진매트릭스 Ｒｎａ 변이 분석방법
MX350658B (es) 2011-09-07 2017-09-13 Human Genetic Signatures Pty Ltd Ensayo de detección molecular.
WO2013035875A1 (ja) 2011-09-08 2013-03-14 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
EP2758547B1 (de) 2011-09-21 2015-09-16 Gen-Probe Incorporated Verfahren zur amplifikation von nukleinsäuren unter verwendung von tag-vermittelter verschiebung
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
SG10201504490QA (en) 2012-01-26 2015-07-30 Nugen Technologies Inc Compositions And Methods For Targeted Nucleic Acid Sequence Enrichment And High Efficiency Library Generation
SG11201408478QA (en) 2012-06-18 2015-02-27 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN102943100B (zh) * 2012-11-23 2014-04-16 郑州安图生物工程股份有限公司 高效RNA逆转录合成cDNA的方法
WO2014109845A1 (en) * 2012-12-03 2014-07-17 Yilin Zhang Single-stranded polynucleotide amplification methods
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
EA037029B1 (ru) 2014-03-31 2021-01-28 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Флуоресцентно меченная одноцепочечная нуклеиновая кислота и ее применение
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
WO2016053881A1 (en) 2014-10-03 2016-04-07 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
WO2016073353A1 (en) 2014-11-03 2016-05-12 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
GB201617643D0 (en) * 2016-10-18 2016-11-30 Oxford University Innovation Limited Method for complete. uniform and specific amplification of ultra-low amounts of input DNA
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US10724017B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
US10689629B1 (en) 2017-12-06 2020-06-23 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands
US10724083B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
WO2023189403A1 (ja) * 2022-03-31 2023-10-05 国立大学法人埼玉大学 Ｒｎａ／ｄｎａキメラプライマーを用いたｐｃｒ及びその増幅断片連結方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
FR2663949A1 (fr) * 1990-06-29 1992-01-03 Genset Sa Perfectionnements a un procede d'amplification enzymatique in vitro.
AU8997991A (en) * 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
CA2122450C (en) * 1991-11-01 2004-07-13 Charles Phillip Morris Solid phase amplification process
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
JPH06327500A (ja) * 1993-05-20 1994-11-29 Toyobo Co Ltd 核酸配列の増幅方法および検出方法
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
CA2140081C (en) * 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique

Also Published As

Publication number Publication date
FR2737223A1 (fr) 1997-01-31
DE69619959T2 (de) 2002-11-07
EP0787209A1 (de) 1997-08-06
ATE214741T1 (de) 2002-04-15
EP0787209B1 (de) 2002-03-20
WO1997004126A1 (fr) 1997-02-06
CA2200627A1 (fr) 1997-02-06
FR2737223B1 (fr) 1997-09-12
CA2200627C (fr) 2011-05-10
US5824517A (en) 1998-10-20

Similar Documents

Publication Publication Date Title
DE69619959D1 (de) Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer
WO1997004126B1 (fr) Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
DE69523360T2 (de) Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung
WO1995003426A3 (fr) Procede d'amplification d'acides nucleiques par transcription utilisant le deplacement, reactifs et necessaire pour la mise en ×uvre de ce procede
CA2167838A1 (en) Methods for Enhancing Nucleic Acid Amplification
DE69307503T2 (de) Chemisches verfahren zur analyse von dns-sequenzen
WO2003020891A3 (en) Single nucleotide amplification and detection by polymerase
ATE189264T1 (de) Chimärisches oligonukleotid und dessen verwendung zur herstellung von nukleinsäure-transkripten
HK1021206A1 (en) Sequencing by ligation of encoded adaptors
WO1997041260A3 (en) Subtractive amplification
WO1995013396A3 (en) A method for identifying microorganisms, and aids useful thereof
IL102765A (en) Reagents and methods to detect and determine mycobacterium nucleic acids
EP0822256A3 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
FI972238A0 (fi) Terminaaliseen toistumasekvenssiin perustuva monistusmenetelmä
ATE403747T1 (de) Verfahren zur amplifikation von signal- flankierenden sequenzen aus unbekannten, genomischen daten
DE69427876D1 (de) Methode zur detektion einer gezielten nukleinsäure
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
EP1251182A3 (de) Verfahren zur Amplifikation und Erkennung von Nukleinsäure-Zielsequenzen unter Verwendung von thermostabilen Enzymen
DE59601881D1 (de) Verfahren zur früherkennung von hpv-assoziierten karzinomen bzw. von hochgradigen, durch hpv-verursachte dysplasien
SE9403805D0 (sv) Method of preparing oligonucleotide probes or primers, vector therefor and use thereof
DE50209094D1 (de) Vermehrung von ribonukleinsäuren
AU2001256220A1 (en) Specific multiplex analysis of nucleic acids
ATE165870T1 (de) Verfahren zur amplifizierung und/oder nachweis einer nukleinsäuresequenz, nachweisreagenz und deren verwendungen
MY111893A (en) Method for detecting a target nucleic acid
WO2004061126A3 (en) Nucleic acid hybridization assay using bridging sequence

Legal Events

Date Code Title Description
8364 No opposition during term of opposition