DE69626111D1 - Universale primersequenz zur multiplex dna amplifikation - Google Patents

Universale primersequenz zur multiplex dna amplifikation

Info

Publication number
DE69626111D1
DE69626111D1 DE69626111T DE69626111T DE69626111D1 DE 69626111 D1 DE69626111 D1 DE 69626111D1 DE 69626111 T DE69626111 T DE 69626111T DE 69626111 T DE69626111 T DE 69626111T DE 69626111 D1 DE69626111 D1 DE 69626111D1
Authority
DE
Germany
Prior art keywords
dna
primer sequence
present
dna amplification
universal primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69626111T
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English (en)
Other versions
DE69626111T2 (de
Inventor
P Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
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Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23883585&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69626111(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of DE69626111D1 publication Critical patent/DE69626111D1/de
Application granted granted Critical
Publication of DE69626111T2 publication Critical patent/DE69626111T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69626111T 1995-06-07 1996-06-06 Universale primersequenz zur multiplex dna amplifikation Expired - Lifetime DE69626111T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/474,450 US5882856A (en) 1995-06-07 1995-06-07 Universal primer sequence for multiplex DNA amplification
PCT/US1996/009637 WO1996041012A1 (en) 1995-06-07 1996-06-06 Universal primer sequence for multiplex dna amplification

Publications (2)

Publication Number Publication Date
DE69626111D1 true DE69626111D1 (de) 2003-03-13
DE69626111T2 DE69626111T2 (de) 2003-12-24

Family

ID=23883585

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69626111T Expired - Lifetime DE69626111T2 (de) 1995-06-07 1996-06-06 Universale primersequenz zur multiplex dna amplifikation

Country Status (7)

Country Link
US (2) US5882856A (de)
EP (1) EP0832290B1 (de)
JP (1) JPH11507226A (de)
AT (1) ATE232242T1 (de)
CA (1) CA2223729C (de)
DE (1) DE69626111T2 (de)
WO (1) WO1996041012A1 (de)

Families Citing this family (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US6015664A (en) * 1995-11-03 2000-01-18 Mcw Research Foundation Multiplex PCR assay using unequal primer concentrations to detect HPIV 1,2,3 and RSV A,B and influenza virus A, B
US5814498A (en) * 1996-04-29 1998-09-29 Archer Daniels Midland Company Process for the recovery of organic acids and ammonia from their salts
US6294066B1 (en) 1997-01-23 2001-09-25 Archer Daniels Midland Company Apparatus and process for electrodialysis of salts
ES2563643T3 (es) * 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6333179B1 (en) * 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
AU753732B2 (en) 1997-10-23 2002-10-24 Genzyme Corporation Methods for detecting contamination in molecular diagnostics using PCR
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
AU3984699A (en) * 1998-05-12 1999-11-29 Whitehead Institute For Biomedical Research Multiplex dna amplification using chimeric primers
DE19834948A1 (de) * 1998-08-03 2000-02-17 Monika Jecht Verfahren zur spezifischen Detektion von RNA
US7135284B1 (en) * 1999-02-05 2006-11-14 Integrated Dna Technologies, Inc. Primer extension methods for production of high specific activity nucleic acid probes
US6125531A (en) * 1999-03-01 2000-10-03 International Business Machines Corporation Method of making a printed circuit board having filled holes and a fill member for use therewith including reinforcement means
EP1165839A2 (de) * 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
EP1257664A4 (de) * 2000-01-28 2006-04-05 Althea Technologies Inc Verfahren zur analyse der genexpression
US20030219765A1 (en) * 2000-03-23 2003-11-27 Jose Costa Methods for evaluating cancer risk
EP2351853A1 (de) 2000-06-06 2011-08-03 Life Technologies Corporation Methode und Vorrichtungen für multiplex Amplifizierungsreaktionen
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) * 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
DE10065814B4 (de) * 2000-12-22 2004-07-08 Epigenomics Ag Verfahren für die simultane Amplifikation vieler Sequenzen in einer PCR-Reaktion und deren Markierung
US6867021B2 (en) 2001-02-20 2005-03-15 Board Of Trustees Of Michigan State University Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
JP4808365B2 (ja) * 2001-03-02 2011-11-02 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション Ｐｃｒ法
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US20030104421A1 (en) * 2001-05-07 2003-06-05 Colangelo Christopher M. Methods and compositions for nucleic acid amplification
US7297778B2 (en) * 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US20090065471A1 (en) * 2003-02-10 2009-03-12 Faris Sadeg M Micro-nozzle, nano-nozzle, manufacturing methods therefor, applications therefor
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
WO2003025202A2 (en) * 2001-09-19 2003-03-27 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
WO2003033741A1 (en) * 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US7510829B2 (en) * 2001-11-19 2009-03-31 Affymetrix, Inc. Multiplex PCR
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1468117A2 (de) * 2002-01-15 2004-10-20 MATFORSK, Norwegian Food Research Institute Verfahren zur amplifikation von nukleinsäuren
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
WO2003097828A1 (en) * 2002-05-21 2003-11-27 Adgene Co., Ltd. Method of identifying nucleic acid
DE60332948D1 (de) * 2002-07-19 2010-07-22 Althea Technologies Inc Strategien zur genexpressionsanalyse
FR2842534B1 (fr) * 2002-07-19 2006-01-20 Inst Nat Sante Rech Med Amplification multiplex quantitative a l'echelle d'un genome, et applications a la detection de remaniements genomiques
AU2003254298A1 (en) * 2002-08-02 2004-02-23 Stratatech Corporation Species specific dna detection
US20040110138A1 (en) * 2002-11-01 2004-06-10 University Of Ottawa Method for the detection of multiple genetic targets
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
EP3000899A1 (de) * 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplexverstärkung von polynucleotiden
WO2004065628A1 (en) * 2003-01-21 2004-08-05 Guoliang Fu Quantitative multiplex detection of nucleic acids
GB2414797A (en) * 2003-01-21 2005-12-07 Guoliang Fu Quantitative multiplex detection of nucleic acids
JP4280519B2 (ja) * 2003-03-07 2009-06-17 アークレイ株式会社 Ｄｎａ増幅法およびそのためのキット
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
US7670810B2 (en) * 2003-06-20 2010-03-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7824856B2 (en) * 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
WO2005038039A2 (en) * 2003-10-13 2005-04-28 Genaco Biomedical Products, Inc. Method for multiplex primer based amplification of nucleic acids
US7824857B2 (en) * 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer
US7981616B2 (en) * 2004-02-27 2011-07-19 Musc Foundation For Research Development Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription
EP1721011A1 (de) * 2004-03-01 2006-11-15 Applera Corporation Verfahren, zusammensetzungen und kits zur verwendung bei der polynukleotidamplifikation
EP1591534A1 (de) * 2004-04-01 2005-11-02 Stichting Sanquin Bloedvoorziening Verfahren zur Genotypisierung von Antigenen von Blutzellen und ein Kit zur Genotypisierung
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
WO2006085964A2 (en) 2004-06-30 2006-08-17 Applera Corporation Log-linear amplification
EP2272987A3 (de) * 2004-07-09 2012-04-11 University of Pittsburgh of the Commonwealth System of Higher Education Identifizierung von Markern für Speiseröhren-, Dickdarm-, Kopf- und Nackenkrebs und Melanom
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
EP1799858A4 (de) * 2004-09-20 2009-03-04 Univ Pittsburgh Multimodus-multiplexlöschverfahren
ES2302667T1 (es) * 2004-09-22 2008-08-01 University Of The West Of England, Bristol Analisis de genes.
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060199188A1 (en) * 2005-03-03 2006-09-07 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
EP2121956B1 (de) 2006-12-21 2016-08-17 Gen-Probe Incorporated Verfahren und zusammensetzungen zur amplizierung von nukleinsäuren
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
JP5669397B2 (ja) * 2007-03-01 2015-02-12 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ 同族抗体をクローニングするための方法
CA2984820C (en) 2007-04-04 2021-12-07 Ande Corporation Plastic microfluidic separation and detection platforms
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20100248310A1 (en) * 2007-10-22 2010-09-30 Monoquant Pty Ltd. Method of dna amplification
CN107385040B (zh) * 2008-04-03 2022-02-15 艾瑞普特公司 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
CA2735020A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
JP2012529908A (ja) * 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド 法医学的ｄｎａの定量化のための改善された方法
US9169512B2 (en) 2009-07-01 2015-10-27 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2287334A1 (de) * 2009-08-21 2011-02-23 Qiagen GmbH Verfahren zum Nachweis von Zielnukleinsäuren
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
WO2011084757A1 (en) 2009-12-21 2011-07-14 University Of Southern California Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
EP3447155A1 (de) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwichassays in tröpfchen
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
EP2683833B1 (de) * 2011-03-10 2018-09-26 Gen-Probe Incorporated Verfahren zur auswahl und optimierung von oligonukleotid-tagsequenzen
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US20130059738A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
WO2012149438A1 (en) 2011-04-28 2012-11-01 Life Technologies Corporation Methods and compositions for multiplex pcr
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP5299981B1 (ja) * 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
EP4361606A2 (de) 2012-02-03 2024-05-01 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
EP3901243A1 (de) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
ES2761920T3 (es) 2012-08-30 2020-05-21 Gen Probe Inc Amplificación de ácido nucleico multifásica
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
GB201316644D0 (en) 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2016012521A1 (en) 2014-07-23 2016-01-28 Biotech Trentino S.P.A. Apparatus for the cooling of a drinking liquid, in particular drinking water, with innovative cooling system with peltier effect
US11060131B2 (en) 2015-03-25 2021-07-13 Angle Europe Limited Solid phase nucleic acid target capture and replication using strand displacing polymerases
WO2016172126A2 (en) 2015-04-20 2016-10-27 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
US11655510B2 (en) * 2015-04-20 2023-05-23 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
EP3307908B1 (de) 2015-06-09 2019-09-11 Life Technologies Corporation Verfahren zur molekularen markierung
CA2991401C (en) 2015-07-07 2023-12-05 Pillar Biosciences Inc. Method for reducing primer-dimer amplification
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
EP3436468B1 (de) 2016-04-01 2023-03-22 Chromacode, Inc. Kompetitive sonden zur erzeugung von techniksignalen
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
GB201621477D0 (en) 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
CN114096683A (zh) 2019-07-03 2022-02-25 简·探针公司 用于确定样品中阴道毛滴虫的存在的寡核苷酸
WO2021126997A1 (en) * 2019-12-18 2021-06-24 Petdx, Inc. Methods and compositions for cancer detection, characterization or management in companion animals
CN114717298A (zh) 2021-01-06 2022-07-08 上海慧众同康生物科技有限公司 一种多重扩增方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2220941B (en) * 1988-06-24 1991-09-11 Nat Res Dev Fowlpox virus non-essential regions
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
WO1993018177A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia Diagnosis of cystic fibrosis using allele specific multiplex polymerase chain reactions
WO1993018178A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia DIAGNOSIS OF β-THALASSEMIA USING A MULTIPLEX AMPLIFICATION REFRACTORY MUTATION SYSTEM
US5474896A (en) * 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5518901A (en) * 1993-04-19 1996-05-21 Murtagh; James J. Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
DK172394B1 (da) * 1994-02-25 1998-05-18 Micro Matic As Dobbeltventil
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification

Also Published As

Publication number Publication date
EP0832290A1 (de) 1998-04-01
CA2223729C (en) 2004-04-20
JPH11507226A (ja) 1999-06-29
EP0832290B1 (de) 2003-02-05
US6207372B1 (en) 2001-03-27
US5882856A (en) 1999-03-16
AU6163696A (en) 1996-12-30
WO1996041012A1 (en) 1996-12-19
AU703417B2 (en) 1999-03-25
DE69626111T2 (de) 2003-12-24
CA2223729A1 (en) 1996-12-19
ATE232242T1 (de) 2003-02-15

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