DE69626111D1 - Universale primersequenz zur multiplex dna amplifikation - Google Patents

Universale primersequenz zur multiplex dna amplifikation

Info

Publication number
DE69626111D1
DE69626111D1 DE69626111T DE69626111T DE69626111D1 DE 69626111 D1 DE69626111 D1 DE 69626111D1 DE 69626111 T DE69626111 T DE 69626111T DE 69626111 T DE69626111 T DE 69626111T DE 69626111 D1 DE69626111 D1 DE 69626111D1
Authority
DE
Germany
Prior art keywords
dna
primer sequence
present
dna amplification
universal primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69626111T
Other languages
English (en)
Other versions
DE69626111T2 (de
Inventor
P Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
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Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23883585&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69626111(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Application granted granted Critical
Publication of DE69626111D1 publication Critical patent/DE69626111D1/de
Publication of DE69626111T2 publication Critical patent/DE69626111T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
DE69626111T 1995-06-07 1996-06-06 Universale primersequenz zur multiplex dna amplifikation Expired - Lifetime DE69626111T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/474,450 US5882856A (en) 1995-06-07 1995-06-07 Universal primer sequence for multiplex DNA amplification
PCT/US1996/009637 WO1996041012A1 (en) 1995-06-07 1996-06-06 Universal primer sequence for multiplex dna amplification

Publications (2)

Publication Number Publication Date
DE69626111D1 true DE69626111D1 (de) 2003-03-13
DE69626111T2 DE69626111T2 (de) 2003-12-24

Family

ID=23883585

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69626111T Expired - Lifetime DE69626111T2 (de) 1995-06-07 1996-06-06 Universale primersequenz zur multiplex dna amplifikation

Country Status (7)

Country Link
US (2) US5882856A (de)
EP (1) EP0832290B1 (de)
JP (1) JPH11507226A (de)
AT (1) ATE232242T1 (de)
CA (1) CA2223729C (de)
DE (1) DE69626111T2 (de)
WO (1) WO1996041012A1 (de)

Families Citing this family (128)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US6015664A (en) * 1995-11-03 2000-01-18 Mcw Research Foundation Multiplex PCR assay using unequal primer concentrations to detect HPIV 1,2,3 and RSV A,B and influenza virus A, B
US5814498A (en) * 1996-04-29 1998-09-29 Archer Daniels Midland Company Process for the recovery of organic acids and ammonia from their salts
US6294066B1 (en) 1997-01-23 2001-09-25 Archer Daniels Midland Company Apparatus and process for electrodialysis of salts
EP3034626A1 (de) 1997-04-01 2016-06-22 Illumina Cambridge Limited Verfahren zur vervielfältigung von nukleinsäuren
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6333179B1 (en) * 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
WO1999020798A1 (en) 1997-10-23 1999-04-29 Exact Laboratories, Inc. Methods for detecting contamination in molecular diagnostics using pcr
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
WO1999058721A1 (en) * 1998-05-12 1999-11-18 Whitehead Institute For Biomedical Research Multiplex dna amplification using chimeric primers
DE19834948A1 (de) * 1998-08-03 2000-02-17 Monika Jecht Verfahren zur spezifischen Detektion von RNA
US7135284B1 (en) * 1999-02-05 2006-11-14 Integrated Dna Technologies, Inc. Primer extension methods for production of high specific activity nucleic acid probes
US6125531A (en) * 1999-03-01 2000-10-03 International Business Machines Corporation Method of making a printed circuit board having filled holes and a fill member for use therewith including reinforcement means
EP1165839A2 (de) * 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
CA2398107C (en) * 2000-01-28 2013-11-19 Althea Technologies, Inc. Methods for analysis of gene expression
US20030219765A1 (en) * 2000-03-23 2003-11-27 Jose Costa Methods for evaluating cancer risk
EP2351853A1 (de) 2000-06-06 2011-08-03 Life Technologies Corporation Methode und Vorrichtungen für multiplex Amplifizierungsreaktionen
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) * 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
DE10065814B4 (de) * 2000-12-22 2004-07-08 Epigenomics Ag Verfahren für die simultane Amplifikation vieler Sequenzen in einer PCR-Reaktion und deren Markierung
US6867021B2 (en) * 2001-02-20 2005-03-15 Board Of Trustees Of Michigan State University Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
EP1373569A4 (de) * 2001-03-02 2006-02-08 Univ Pittsburgh Pcr-verfahren
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
WO2002090561A1 (en) * 2001-05-07 2002-11-14 Curagen Corporation Methods and composition for nucleic acid amplification
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US7297778B2 (en) * 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
DE60234369D1 (de) 2001-09-19 2009-12-24 Alexion Pharma Inc Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation
WO2003033741A1 (en) * 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
AU2002366098A1 (en) * 2001-11-19 2003-06-10 Parallele Bioscience Inc Multiplex pcr
AU2003205817A1 (en) * 2002-01-15 2003-07-30 Matforsk Methods of nucleic acid amplification
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1510576A4 (de) * 2002-05-21 2007-08-22 G & G Science Co Ltd Verfahren zur identifizierung von nukleinsäuren
ATE470722T1 (de) * 2002-07-19 2010-06-15 Althea Technologies Inc Strategien zur genexpressionsanalyse
FR2842534B1 (fr) * 2002-07-19 2006-01-20 Inst Nat Sante Rech Med Amplification multiplex quantitative a l'echelle d'un genome, et applications a la detection de remaniements genomiques
US7462448B2 (en) * 2002-08-02 2008-12-09 Stratatech Corporation Species specific DNA detection
CA2410795A1 (en) * 2002-11-01 2004-05-01 University Of Ottawa A method for the amplification of multiple genetic targets
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
AU2003298706A1 (en) 2002-12-04 2004-06-23 Applera Corporation Multiplex amplification of polynucleotides
AU2003202671A1 (en) * 2003-01-21 2004-08-13 Guoliang Fu Quantitative multiplex detection of nucleic acids
WO2004065628A1 (en) * 2003-01-21 2004-08-05 Guoliang Fu Quantitative multiplex detection of nucleic acids
WO2004071948A2 (en) * 2003-02-10 2004-08-26 Reveo, Inc. Micro-nozzle, nano-nozzle, manufacturing methods therefor, applications therefor
JP4280519B2 (ja) * 2003-03-07 2009-06-17 アークレイ株式会社 Ｄｎａ増幅法およびそのためのキット
US7670810B2 (en) * 2003-06-20 2010-03-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7824856B2 (en) * 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
AU2004282555B2 (en) * 2003-10-13 2009-05-28 Diatherix Laboratories, Inc. Method and kit for primer based amplification of nucleic acids
US7824857B2 (en) * 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer
US7981616B2 (en) * 2004-02-27 2011-07-19 Musc Foundation For Research Development Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription
WO2005085475A1 (en) * 2004-03-01 2005-09-15 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
EP1591534A1 (de) * 2004-04-01 2005-11-02 Stichting Sanquin Bloedvoorziening Verfahren zur Genotypisierung von Antigenen von Blutzellen und ein Kit zur Genotypisierung
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
WO2006085964A2 (en) 2004-06-30 2006-08-17 Applera Corporation Log-linear amplification
JP2008505644A (ja) 2004-07-09 2008-02-28 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション 食道癌、結腸癌、頭頸部癌、およびメラノーマにおけるマーカーの同定
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
JP2008515393A (ja) * 2004-09-20 2008-05-15 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション 複数モードの多重化反応消去方法
CA2581240A1 (en) * 2004-09-22 2006-03-30 University Of The West Of England, Bristol Rhd and abo genotyping by multiplex pcr
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060199188A1 (en) * 2005-03-03 2006-09-07 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
EP2363205A3 (de) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen Und Verfahren Zur Verwendung Bei Der Bildung Und Kontrolle Von Nanoreaktoren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (de) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
JP5340167B2 (ja) * 2006-12-21 2013-11-13 ジェン−プロウブ インコーポレイテッド 核酸増幅のための方法および組成物
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
PT2121920E (pt) * 2007-03-01 2011-10-03 Symphogen As Método de clonagem de anticorpos cognatos
KR101530943B1 (ko) * 2007-04-04 2015-06-23 네트바이오, 인코포레이티드 통합된 핵산 분석
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20100248310A1 (en) * 2007-10-22 2010-09-30 Monoquant Pty Ltd. Method of dna amplification
PT2271767T (pt) * 2008-04-03 2016-08-18 Cb Biotechnologies Inc Reação em cadeia de polimerase para captura de amplicões em multiplexo para amplificação de alvos múltiplos
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
JP2012500634A (ja) * 2008-08-29 2012-01-12 シムフォゲン・アクティーゼルスカブ 鳥類から得られた抗体のクローニング方法
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
US9169512B2 (en) 2009-07-01 2015-10-27 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2287334A1 (de) * 2009-08-21 2011-02-23 Qiagen GmbH Verfahren zum Nachweis von Zielnukleinsäuren
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
US20120288861A1 (en) 2009-12-21 2012-11-15 Heinz-Josef Lenz Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
EP2534267B1 (de) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digitale analyse von analyten
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012122571A1 (en) * 2011-03-10 2012-09-13 Gen-Probe Incorporated Methods and compositions for the selection and optimization of oligonucleotide tag sequences
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
EP3072977B1 (de) 2011-04-28 2018-09-19 Life Technologies Corporation Verfahren und zusammensetzungen für multiplex-pcr
US20130059738A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
EP3709018A1 (de) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP5299981B1 (ja) * 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
US8838394B2 (en) 2012-02-03 2014-09-16 California Institute Of Technology Signal encoding and decoding in multiplexed biochemical assays
ES2761920T3 (es) 2012-08-30 2020-05-21 Gen Probe Inc Amplificación de ácido nucleico multifásica
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
GB201316644D0 (en) 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US10557650B2 (en) 2014-07-23 2020-02-11 Biotech Trentino S.P.A. Apparatus for the cooling of a drinking liquid, in particular drinking water, with innovative cooling system with peltier effect
WO2016149837A1 (en) 2015-03-25 2016-09-29 Axela Inc. Solid phase nucleic acid target capture and replication using strand displacing polymerases
US10975440B2 (en) 2015-04-20 2021-04-13 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
US11655510B2 (en) * 2015-04-20 2023-05-23 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
KR102625365B1 (ko) * 2015-07-07 2024-01-15 필러 바이오사이언시스 인코포레이티드 프라이머-다이머 증폭 감소 방법
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
GB201621477D0 (en) 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
JP2022540801A (ja) 2019-07-03 2022-09-20 ジェン－プローブ・インコーポレーテッド 試料中のＴｒｉｃｈｏｍｏｎａｓ ｖａｇｉｎａｌｉｓの存在を決定する際に使用するためのオリゴヌクレオチド
WO2021126997A1 (en) * 2019-12-18 2021-06-24 Petdx, Inc. Methods and compositions for cancer detection, characterization or management in companion animals
CN114717298A (zh) 2021-01-06 2022-07-08 上海慧众同康生物科技有限公司 一种多重扩增方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012684A1 (en) * 1988-06-24 1989-12-28 National Research Development Corporation Fowlpox virus non-essential regions
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
WO1993018177A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia Diagnosis of cystic fibrosis using allele specific multiplex polymerase chain reactions
WO1993018178A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia DIAGNOSIS OF β-THALASSEMIA USING A MULTIPLEX AMPLIFICATION REFRACTORY MUTATION SYSTEM
US5474896A (en) * 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5518901A (en) * 1993-04-19 1996-05-21 Murtagh; James J. Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
DK172394B1 (da) * 1994-02-25 1998-05-18 Micro Matic As Dobbeltventil
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification

Also Published As

Publication number Publication date
CA2223729A1 (en) 1996-12-19
EP0832290B1 (de) 2003-02-05
JPH11507226A (ja) 1999-06-29
AU6163696A (en) 1996-12-30
AU703417B2 (en) 1999-03-25
CA2223729C (en) 2004-04-20
US5882856A (en) 1999-03-16
DE69626111T2 (de) 2003-12-24
US6207372B1 (en) 2001-03-27
EP0832290A1 (de) 1998-04-01
ATE232242T1 (de) 2003-02-15
WO1996041012A1 (en) 1996-12-19

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