DE69627181D1 - Verfahren und vorrichtung zum identifizieren von fluorophoren - Google Patents
Verfahren und vorrichtung zum identifizieren von fluorophorenInfo
- Publication number
- DE69627181D1 DE69627181D1 DE69627181T DE69627181T DE69627181D1 DE 69627181 D1 DE69627181 D1 DE 69627181D1 DE 69627181 T DE69627181 T DE 69627181T DE 69627181 T DE69627181 T DE 69627181T DE 69627181 D1 DE69627181 D1 DE 69627181D1
- Authority
- DE
- Germany
- Prior art keywords
- fluorophores
- fluorophore
- fluorescence
- present
- identifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/561,368 US5784157A (en) | 1995-11-21 | 1995-11-21 | Method and apparatus for identifying fluorophores |
PCT/US1996/018696 WO1997019342A1 (en) | 1995-11-21 | 1996-11-20 | Method and apparatus for identifying fluorophores |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69627181D1 true DE69627181D1 (de) | 2003-05-08 |
DE69627181T2 DE69627181T2 (de) | 2004-01-29 |
Family
ID=24241654
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69627181T Expired - Lifetime DE69627181T2 (de) | 1995-11-21 | 1996-11-20 | Verfahren und vorrichtung zum identifizieren von fluorophoren |
Country Status (7)
Country | Link |
---|---|
US (1) | US5784157A (de) |
EP (1) | EP0862735B1 (de) |
AT (1) | ATE236389T1 (de) |
AU (1) | AU1080097A (de) |
CA (1) | CA2237222C (de) |
DE (1) | DE69627181T2 (de) |
WO (1) | WO1997019342A1 (de) |
Families Citing this family (61)
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US6304771B1 (en) * | 1993-10-29 | 2001-10-16 | The Trustees Of The University Of Pennsylvania | Systems and methods for imaging fluorophores |
US7328059B2 (en) * | 1996-08-23 | 2008-02-05 | The Texas A & M University System | Imaging of light scattering tissues with fluorescent contrast agents |
US6766183B2 (en) | 1995-11-22 | 2004-07-20 | Medtronic Minimed, Inc. | Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers |
US6445448B1 (en) * | 1997-03-12 | 2002-09-03 | Corning Applied Technologies, Corp. | System and method for molecular sample measurement |
LU90186B1 (fr) * | 1997-12-22 | 1999-06-24 | Communaute Europ Ce Batiment E | Dispositif d'analyse non destructive de plantes et véhicule comportant un tel dispositif embarqué |
US6369893B1 (en) * | 1998-05-19 | 2002-04-09 | Cepheid | Multi-channel optical detection system |
US6818437B1 (en) | 1998-05-16 | 2004-11-16 | Applera Corporation | Instrument for monitoring polymerase chain reaction of DNA |
US7498164B2 (en) * | 1998-05-16 | 2009-03-03 | Applied Biosystems, Llc | Instrument for monitoring nucleic acid sequence amplification reaction |
AU759974B2 (en) | 1998-05-16 | 2003-05-01 | Applied Biosystems, Llc | Instrument for monitoring polymerase chain reaction of DNA |
US6528801B1 (en) * | 1998-11-04 | 2003-03-04 | The Research Foundation Of State University Of New York | Method and apparatus for detecting radiation |
US6497804B1 (en) * | 1998-12-03 | 2002-12-24 | Research Foundation Of The State University Of New York | Method and apparatus for DNA sequencing |
DE19915137C2 (de) * | 1999-03-26 | 2001-10-18 | Michael Schaefer | Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens |
DE19935766A1 (de) * | 1999-07-29 | 2001-02-01 | Friedrich Schiller Uni Jena Bu | Verfahren zur optischen Anregung von Fluorophor-markierter DNA und RNA |
US6238830B1 (en) | 1999-10-29 | 2001-05-29 | Advanced Micro Devices | Active control of temperature in scanning probe lithography and maskless lithograpy |
EP1257664A4 (de) * | 2000-01-28 | 2006-04-05 | Althea Technologies Inc | Verfahren zur analyse der genexpression |
JP2001255301A (ja) * | 2000-03-08 | 2001-09-21 | Shimadzu Corp | 電気泳動装置 |
US20020037149A1 (en) * | 2000-03-13 | 2002-03-28 | Shiping Chen | Fiber optic scanner |
US7630063B2 (en) * | 2000-08-02 | 2009-12-08 | Honeywell International Inc. | Miniaturized cytometer for detecting multiple species in a sample |
AU2001282115A1 (en) * | 2000-08-22 | 2002-03-04 | Evotec Oai Ag | Method and device for the measurement of chemical and/or biological samples |
DE10040988A1 (de) * | 2000-08-22 | 2002-03-21 | Evotec Biosystems Ag | Verfahren und Vorrichtung zum Messen chemischer und/oder biologischer Proben |
DE60220497T2 (de) | 2001-01-26 | 2008-01-31 | Biocal Technology, Inc., Irvine | Optische detektion in einem mehrkanaligen bioseparationssystem |
AU2002251944A1 (en) * | 2001-02-15 | 2002-09-04 | Medtronic Minimed, Inc. | Polymers functionalized with fluorescent boronate motifs |
US6897959B2 (en) | 2001-04-13 | 2005-05-24 | Menlo Biocombs, Inc. | Frequency comb analysis |
US20030110840A1 (en) * | 2001-07-24 | 2003-06-19 | Arriaga Edgar A. | Systems and methods for detecting a particle |
CA2453390A1 (en) * | 2001-07-25 | 2003-02-06 | Applera Corporation | Time-delay integration in electrophoretic detection systems |
US7280207B2 (en) * | 2001-07-25 | 2007-10-09 | Applera Corporation | Time-delay integration in a flow cytometry system |
US7265833B2 (en) * | 2001-07-25 | 2007-09-04 | Applera Corporation | Electrophoretic system with multi-notch filter and laser excitation source |
US6995841B2 (en) * | 2001-08-28 | 2006-02-07 | Rice University | Pulsed-multiline excitation for color-blind fluorescence detection |
US7045361B2 (en) | 2001-09-12 | 2006-05-16 | Medtronic Minimed, Inc. | Analyte sensing via acridine-based boronate biosensors |
US6870165B2 (en) * | 2001-10-19 | 2005-03-22 | Biocal Technology, Inc. | Multi-color multiplexed analysis in a bio-separation system |
US20070096039A1 (en) * | 2002-05-03 | 2007-05-03 | Rakesh Kapoor | Evaluation Of Multicomponent Mixtures Using Modulated Light Beams |
US20030205682A1 (en) * | 2002-05-03 | 2003-11-06 | Rakesh Kapoor | Evaluation of multicomponent mixtures using modulated light beams |
US20040126275A1 (en) * | 2002-07-31 | 2004-07-01 | Klaus Doering | Method and device for measuring the lifetime of the fluorescence of fluorophores in samples |
US6934030B2 (en) * | 2002-12-18 | 2005-08-23 | The Research Foundation Of State University Of New York | Method and apparatus for detecting radiation |
EP1671109A4 (de) * | 2003-06-20 | 2007-09-19 | Univ California | Modulierte anregungsfluoreszenzanalyse |
JP4224640B2 (ja) * | 2004-03-29 | 2009-02-18 | オリンパス株式会社 | 蛍光分光分析装置 |
US7351538B2 (en) * | 2004-08-23 | 2008-04-01 | U.S. Genomics | Systems and methods for detecting and analyzing polymers |
CA2842232C (en) | 2005-03-10 | 2015-01-27 | Gen-Probe Incorporated | Systems and methods to perform assays for detecting or quantifying analytes within samples |
DE102005022880B4 (de) * | 2005-05-18 | 2010-12-30 | Olympus Soft Imaging Solutions Gmbh | Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern |
EP1893756B1 (de) | 2005-06-08 | 2015-08-12 | The Regents of The University of California | Entfernung von n-glycolylneuraminsäure aus säugerprodukten für menschliche verwendung |
WO2007002490A2 (en) | 2005-06-22 | 2007-01-04 | The Research Foundation Of State University Of New York | Massively parallel 2-dimensional capillary electrophoresis |
JP4829716B2 (ja) * | 2006-08-18 | 2011-12-07 | シスメックス株式会社 | 血液凝固分析装置 |
US7924421B2 (en) * | 2006-09-01 | 2011-04-12 | Agr International, Inc. | In-line inspection system for vertically profiling plastic containers using multiple wavelength discrete spectral light sources |
GB2445956B (en) * | 2007-01-26 | 2009-12-02 | Valtion Teknillinen | A spectrometer and a method for controlling the spectrometer |
US8101426B2 (en) * | 2007-03-02 | 2012-01-24 | Icyt Mission Technology, Inc. | System and method for the measurement of multiple fluorescence emissions in a flow cytometry system |
CA2691451C (en) * | 2007-06-21 | 2015-03-24 | Sara H. Fan | Instrument and receptacles for performing processes |
JP2011501189A (ja) * | 2007-10-25 | 2011-01-06 | ザ・リサーチ・ファウンデーション・オブ・ステイト・ユニバーシティー・オブ・ニューヨーク | 単一光子分光計 |
US20100032582A1 (en) * | 2008-08-07 | 2010-02-11 | General Electric Company | Fluorescence detection system and method |
KR20110125640A (ko) * | 2009-01-21 | 2011-11-21 | 레어 라이트, 인크. | 다중 분리형 광원을 사용하는 라만 분광 장치, 시스템 및 방법 |
WO2010091046A2 (en) * | 2009-02-03 | 2010-08-12 | President & Fellows Of Harvard College | Systems and methods for high throughput, high fidelity, single molecule nucleic acid sequencing using time multiplexed excitation |
US8254650B2 (en) * | 2009-02-27 | 2012-08-28 | General Electric Company | System and method for contrast enhancement of time-resolved fluorescence images |
MX2011010635A (es) | 2009-04-07 | 2012-04-02 | Rare Light Inc | Dispositivos, sistemas y metodos de espectroscopia de reflexion peri-critica. |
RU2525706C2 (ru) * | 2009-04-15 | 2014-08-20 | Конинклейке Филипс Электроникс Н.В. | Устройство для регулируемого по времени определения флуоресценции |
US20100293457A1 (en) * | 2009-05-15 | 2010-11-18 | Gemstar Development Corporation | Systems and methods for alphanumeric navigation and input |
US9222830B2 (en) * | 2010-06-17 | 2015-12-29 | Research Foundation Of The State University Of New York | Optical control sensor system with driver that switches frequencies of a set of different orthogonal signals and modulates the intensity of light |
US8729502B1 (en) | 2010-10-28 | 2014-05-20 | The Research Foundation For The State University Of New York | Simultaneous, single-detector fluorescence detection of multiple analytes with frequency-specific lock-in detection |
CN103403533B (zh) | 2011-02-24 | 2017-02-15 | 简.探针公司 | 用于分辨光信号检测器中不同调制频率的光信号的系统和方法 |
US9606060B2 (en) | 2012-01-13 | 2017-03-28 | California Institute Of Technology | Filterless time-domain detection of one or more fluorophores |
GB2569329B (en) * | 2017-12-13 | 2021-06-09 | Univ Oxford Innovation Ltd | Apparatus for optical detection of contamination, radiation source, method for optical detection of contamination |
CN108152262A (zh) * | 2018-01-11 | 2018-06-12 | 南京溯远基因科技有限公司 | 一种毛细管电泳核酸分析方法及系统 |
DE102020127385B3 (de) | 2020-10-16 | 2022-03-10 | Abberior Instruments Gmbh | Verfahren und Vorrichtung zur lichtmikroskopischen Multiskalen-Aufnahme biologischer Proben |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5207880A (en) * | 1984-03-29 | 1993-05-04 | The Board Of Regents Of The University Of Nebraska | DNA sequencing |
US4791310A (en) * | 1986-10-02 | 1988-12-13 | Syracuse University | Fluorescence microscopy |
US4800885A (en) * | 1987-12-02 | 1989-01-31 | The Boc Group, Inc. | Blood constituent monitoring apparatus and methods with frequency division multiplexing |
AT391030B (de) * | 1988-12-01 | 1990-08-10 | Avl Verbrennungskraft Messtech | Vorrichtung zur messung chemischer und physikalischer parameter eines fluessigen oder gasfoermigen mediums |
US4937457A (en) * | 1989-02-10 | 1990-06-26 | Slm Instruments, Inc. | Picosecond multi-harmonic fourier fluorometer |
JPH0670613B2 (ja) * | 1989-04-03 | 1994-09-07 | 浜松ホトニクス株式会社 | 光波形測定装置 |
US5244810A (en) * | 1990-01-12 | 1993-09-14 | Gottlieb Amos J | Analytical method |
US5281825A (en) * | 1991-09-05 | 1994-01-25 | The University Of Maryland School Of Medicine | Phase fluorometry using a modulated electroluminescent lamp as a light source |
US5300789A (en) * | 1991-12-24 | 1994-04-05 | At&T Bell Laboratories | Article comprising means for modulating the optical transparency of a semiconductor body, and method of operating the article |
US5321253A (en) * | 1991-12-31 | 1994-06-14 | Biota Corp. | Method of and means for controlling the electromagnetic output power of electro-optic semiconductor devices |
US5422904A (en) * | 1991-12-31 | 1995-06-06 | Biota Corp. | Method of and means for controlling the electromagnetic output power of electro-optic semiconductor devices |
US5274225A (en) * | 1991-12-31 | 1993-12-28 | Biota Corp. | Method of and means for controlling the electromagnetic output power of electro-optic semiconductor devices |
DE4210970C2 (de) * | 1992-04-02 | 1996-10-17 | Markus Dipl Chem Sauer | Verfahren zur simultanen optischen qualitativen und quantitativen Erfassung von verschiedenen mit Fluorochromen oder Fluorogenen markierten Molekülen eines Gemisches mittels Laserspektroskopie |
US5418371A (en) * | 1993-02-01 | 1995-05-23 | Aslund; Nils R. D. | Apparatus for quantitative imaging of multiple fluorophores using dual detectors |
US5365326A (en) * | 1993-02-12 | 1994-11-15 | The Dow Chemical Company | Method and apparatus for determining flow rates and concentrations in multi-phase streams |
US5311526A (en) * | 1993-02-25 | 1994-05-10 | At&T Bell Laboratories | Article that comprises a semiconductor laser, and method of operating the article |
-
1995
- 1995-11-21 US US08/561,368 patent/US5784157A/en not_active Expired - Lifetime
-
1996
- 1996-11-20 AU AU10800/97A patent/AU1080097A/en not_active Abandoned
- 1996-11-20 EP EP96940838A patent/EP0862735B1/de not_active Expired - Lifetime
- 1996-11-20 AT AT96940838T patent/ATE236389T1/de not_active IP Right Cessation
- 1996-11-20 WO PCT/US1996/018696 patent/WO1997019342A1/en active IP Right Grant
- 1996-11-20 CA CA002237222A patent/CA2237222C/en not_active Expired - Fee Related
- 1996-11-20 DE DE69627181T patent/DE69627181T2/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5784157A (en) | 1998-07-21 |
WO1997019342A1 (en) | 1997-05-29 |
AU1080097A (en) | 1997-06-11 |
CA2237222A1 (en) | 1997-05-29 |
DE69627181T2 (de) | 2004-01-29 |
ATE236389T1 (de) | 2003-04-15 |
EP0862735A1 (de) | 1998-09-09 |
CA2237222C (en) | 2003-07-01 |
EP0862735B1 (de) | 2003-04-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |