DE69731285D1 - Langwellig fluorezierende proteine - Google Patents

Langwellig fluorezierende proteine

Info

Publication number
DE69731285D1
DE69731285D1 DE69731285T DE69731285T DE69731285D1 DE 69731285 D1 DE69731285 D1 DE 69731285D1 DE 69731285 T DE69731285 T DE 69731285T DE 69731285 T DE69731285 T DE 69731285T DE 69731285 D1 DE69731285 D1 DE 69731285D1
Authority
DE
Germany
Prior art keywords
fluorescent proteins
long
wave fluorescent
wave
image
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69731285T
Other languages
English (en)
Other versions
DE69731285T2 (de
Inventor
Y Tsien
B Cubitt
Roger Heim
F Ormo
S Remington
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oregon State Board of Higher Education
University of California
Life Technologies Corp
Oregon State
Original Assignee
Oregon State Board of Higher Education
University of California
Invitrogen Corp
Oregon State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oregon State Board of Higher Education, University of California, Invitrogen Corp, Oregon State filed Critical Oregon State Board of Higher Education
Publication of DE69731285D1 publication Critical patent/DE69731285D1/de
Application granted granted Critical
Publication of DE69731285T2 publication Critical patent/DE69731285T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2525—Stabilizing or preserving
DE69731285T 1996-08-16 1997-08-15 Langwellig fluorezierende proteine Expired - Lifetime DE69731285T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US2405096P 1996-08-16 1996-08-16
US24050P 1996-08-16
US08/706,408 US6124128A (en) 1996-08-16 1996-08-30 Long wavelength engineered fluorescent proteins
US706408 1996-08-30
PCT/US1997/014593 WO1998006737A1 (en) 1996-08-16 1997-08-15 Long wavelength engineered fluorescent proteins

Publications (2)

Publication Number Publication Date
DE69731285D1 true DE69731285D1 (de) 2004-11-25
DE69731285T2 DE69731285T2 (de) 2006-03-09

Family

ID=26697970

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69731285T Expired - Lifetime DE69731285T2 (de) 1996-08-16 1997-08-15 Langwellig fluorezierende proteine

Country Status (10)

Country Link
US (6) US6124128A (de)
EP (2) EP0886644B1 (de)
JP (1) JP4232992B2 (de)
AT (1) ATE280178T1 (de)
AU (1) AU727088B2 (de)
CA (1) CA2232242C (de)
DE (1) DE69731285T2 (de)
DK (1) DK0886644T3 (de)
ES (1) ES2231890T3 (de)
WO (1) WO1998006737A1 (de)

Families Citing this family (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4482996A (en) * 1995-09-22 1997-04-09 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
WO1998021355A1 (en) 1996-11-15 1998-05-22 Life Technologies, Inc. Mutants of green fluorescent protein
US6670449B1 (en) 1997-02-13 2003-12-30 Memorial Sloan-Kettering Cancer Center Hybrid molecules and their use for optically detecting changes in cellular microenvironments
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
ATE412050T1 (de) * 1997-03-14 2008-11-15 Univ California Fluoreszenzprotein-sensoren zur erkennung von analyten
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
US6881683B2 (en) * 1998-02-05 2005-04-19 Asm Japan K.K. Insulation film on semiconductor substrate and method for forming same
US7090976B2 (en) * 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
US6140132A (en) * 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6150176A (en) * 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
EP1102977A1 (de) * 1998-08-08 2001-05-30 Imperial Cancer Research Technology Limited Fluoreszenz-assay für biologische systeme
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
US6670144B1 (en) 1999-02-26 2003-12-30 Cyclacel, Ltd. Compositions and methods for monitoring the phosphorylation of natural binding partners
US6828106B2 (en) * 1999-02-26 2004-12-07 Cyclacel Limited Methods and compositions using coiled binding partners
US6972182B1 (en) * 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using coiled binding partners
US6716614B1 (en) 1999-09-02 2004-04-06 Lexicon Genetics Incorporated Human calcium dependent proteases, polynucleotides encoding the same, and uses thereof
US6541252B1 (en) 2000-05-19 2003-04-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6562563B1 (en) 1999-11-03 2003-05-13 Mitokor Compositions and mehtods for determining interactions of mitochondrial components, and for identifying agents that alter such interactions
US7060506B2 (en) 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
US6656700B2 (en) * 2000-05-26 2003-12-02 Amersham Plc Isoforms of human pregnancy-associated protein-E
US6686188B2 (en) 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
DE60139556D1 (de) * 2000-06-19 2009-09-24 Fisher Bioimage Aps Fluoreszierende proteine
US20040072995A1 (en) * 2000-06-19 2004-04-15 Bjorn Sara P. Novel fluorescent proteins
US6667153B1 (en) 2000-06-26 2003-12-23 Susan Margaret Thomas Composition and method for detecting mutagens
US20020123474A1 (en) * 2000-10-04 2002-09-05 Shannon Mark E. Human GTP-Rho binding protein2
EP1409674A2 (de) * 2000-10-27 2004-04-21 Lexicon Genetics Incorporated Humane 7tm proteine und dafür kodierende polynukleotide
CA2427701A1 (en) * 2000-10-30 2002-05-10 Lexicon Genetics Incorporated Human 7tm proteins and polynucleotides encoding the same
WO2002040874A1 (en) 2000-11-16 2002-05-23 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7005511B2 (en) 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
JP3929250B2 (ja) * 2001-03-08 2007-06-13 株式会社ルネサステクノロジ 半導体装置
US7015310B2 (en) 2001-03-12 2006-03-21 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation reduction sensitive green fluorescent protein variants
GB0109858D0 (en) * 2001-04-23 2001-06-13 Amersham Pharm Biotech Uk Ltd Fluorscent proteins
US20030044847A1 (en) * 2001-05-15 2003-03-06 Sidney Pestka Methods for anlyzing interactions between proteins in live and intact cells
AU2002309989A1 (en) * 2001-05-18 2002-12-03 Rigel Pharmaceuticals, Incorporated Directed evolution of protein in mammalian cells
US7354735B2 (en) * 2001-05-24 2008-04-08 Hong Kong University Of Science And Technology Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
US20040096880A1 (en) * 2001-08-07 2004-05-20 Kmiec Eric B. Compositions and methods for the treatment of diseases exhibiting protein misassembly and aggregation
CA2455424A1 (en) * 2001-08-07 2003-02-20 University Of Delaware Compositions and methods for the prevention and treatment of huntington's disease
US6900289B2 (en) * 2001-08-22 2005-05-31 The University Of Hawaii Physalia fluorescent proteins
US20030027315A1 (en) * 2001-09-24 2003-02-06 Ada Yonath Methods of growing crystals of free and antibiotic complexed large ribosomal subunits, and methods of rationally designing or identifying antibiotics using structure coordinate data derived from such crystals
CA2462598A1 (en) * 2001-10-01 2003-04-10 Bioimage A/S A method of detecting intracellular protein-protein interactions using three heterologous conjugates
GB0123856D0 (en) * 2001-10-05 2001-11-28 Amersham Pharm Biotech Uk Ltd Method for determining cell cycle position
WO2003040312A2 (en) * 2001-10-24 2003-05-15 University Of Hawaii Multifunctional green fluorescent protein and uses thereof
AU2002350039A1 (en) * 2001-10-29 2003-05-12 Renovis, Inc. Method for isolating cell-type specific mrnas
US7846671B2 (en) 2002-01-28 2010-12-07 Bristol-Myers Squibb Company Methods of screening for agents that modulate the interaction of RGS and Gαq and urinary incontinence
GB0207533D0 (en) 2002-04-02 2002-05-08 Oxford Glycosciences Uk Ltd Protein
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
US7335488B2 (en) * 2002-04-05 2008-02-26 The Chinese University Of Hong Kong Fluorescent proteins
CA2899360A1 (en) 2002-08-21 2004-04-08 Revivicor, Inc. Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US7056683B2 (en) * 2002-11-12 2006-06-06 Massachusetts Institute Of Technology Genetically encoded fluorescent reporters of kinase, methyltransferase, and acetyl-transferase activities
WO2004090115A2 (en) 2003-04-04 2004-10-21 Stratagene Renilla gfp mutants with increased fluorescent intensity and special shift
US20050037364A1 (en) * 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
US20050048493A1 (en) * 2003-08-27 2005-03-03 Zyomyx, Inc. Film layer for detection of immobilized analytes
US7279275B2 (en) 2003-11-03 2007-10-09 Washington University Methods and compositions for detection of segmented negative strand RNA viruses
BRPI0513826A2 (pt) 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
WO2006060739A2 (en) 2004-12-01 2006-06-08 Proteologics, Inc. Ubiquitin ligase assays and related reagents
AU2006340711C1 (en) 2005-04-05 2013-02-07 Allergan, Inc. Clostridial toxin activity assays
EP2949668B1 (de) 2005-05-18 2019-08-14 Ablynx N.V. Verbesserte nanokörper-tm gegen tumornekrosefaktor alpha
CA2608873C (en) 2005-05-20 2017-04-25 Ablynx Nv Single domain vhh antibodies against von willebrand factor
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
AU2006292827B2 (en) 2005-08-09 2013-02-14 Revivicor, Inc. Transgenic ungulates expressing CTLA4-IG and uses thereof
WO2007052102A2 (en) * 2005-11-04 2007-05-10 Evrogen, Jsc Modified green fluorescent proteins and methods for using same
PL2347775T3 (pl) 2005-12-13 2020-11-16 President And Fellows Of Harvard College Rusztowania do przeszczepiania komórek
EP1988161B1 (de) * 2006-01-26 2012-03-07 NEC Soft, Ltd. Verfahren zur änderung der fluoreszenzwellenlänge eines fluoroproteins
JP2009530423A (ja) * 2006-03-21 2009-08-27 ワイス アミロイド生成性疾患を予防および処置するための方法
GB0606430D0 (en) * 2006-03-31 2006-05-10 Ge Healthcare Uk Ltd Method for cell based assays
US20080004410A1 (en) * 2006-06-30 2008-01-03 Yu-Chin Lai Hydrophilic macromonomers having alpha,beta-conjugated carboxylic terminal group and medical devices incorporating same
GB0624462D0 (en) * 2006-12-07 2007-01-17 Ge Healthcare Ltd Method for determining gentoxicity
WO2008074839A2 (en) 2006-12-19 2008-06-26 Ablynx N.V. Amino acid sequences directed against gpcrs and polypeptides comprising the same for the treatment of gpcr-related diseases and disorders
EP2514767A1 (de) 2006-12-19 2012-10-24 Ablynx N.V. Gegen Metalloproteinase der ADAM-Familie gerichtete Aminosäuren und Polypeptide damit zur Behandlung von Erkrankungen und Störungen im Zusammenhang mit ADAM
WO2008114544A1 (ja) * 2007-03-16 2008-09-25 Tokyo Metropolitan Organization For Medical Research 細胞周期可視化プローブ
CN101688213A (zh) 2007-04-27 2010-03-31 陶氏环球技术公司 用于快速筛选微生物宿主以鉴定某些在表达异源蛋白质方面具有改善的产量和/或质量的菌株的方法
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2009002401A2 (en) 2007-06-21 2008-12-31 President And Fellows Of Harvard College Scaffolds for cell collection or elimination
CN101946171B (zh) * 2007-12-14 2014-03-12 拜奥蒂乌姆股份有限公司 荧光化合物
GB0800938D0 (en) * 2008-01-18 2008-02-27 Ge Healthcare Uk Ltd Multiplex cell signalling assay
US9370558B2 (en) 2008-02-13 2016-06-21 President And Fellows Of Harvard College Controlled delivery of TLR agonists in structural polymeric devices
JP5690143B2 (ja) 2008-02-13 2015-03-25 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 持続的細胞プログラミング装置
US20110071049A1 (en) * 2008-03-12 2011-03-24 Nathaniel Heintz Methods and compositions for translational profiling and molecular phenotyping
EP2260058A2 (de) 2008-04-07 2010-12-15 Ablynx N.V. Variable einzeldomänen gegen notch-signalwege
WO2009146456A1 (en) 2008-05-30 2009-12-03 President And Fellows Of Harvard College Controlled release of growth factors and signaling molecules for promoting angiogenesis
US20100017232A1 (en) * 2008-07-18 2010-01-21 StevenDale Software, LLC Information Transmittal And Notification System
JP5654989B2 (ja) * 2008-07-22 2015-01-14 フィブロスタチン ソシエダ リミターダＦｉｂｒｏｓｔａｔｉｎ，Ｓｏｃｉｅｄａｄ Ｌｉｍｉｔａｄａ グッドパスチャー抗原結合タンパク質およびその検出
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
CN102257003B (zh) 2008-12-19 2017-04-05 埃博灵克斯股份有限公司 用于产生针对细胞相关抗原如p2x7、cxcr7或cxcr4的免疫球蛋白的基因免疫
JP5647222B2 (ja) 2009-04-10 2014-12-24 アブリンクス エン．ヴェー． Ｉｌ−６ｒ関連疾患及び障害の治療のためのｉｌ−６ｒに指向性を有する改善されたアミノ酸配列及びこれを含むポリペプチド
WO2010120749A2 (en) 2009-04-13 2010-10-21 President And Fellow Of Harvard College Harnessing cell dynamics to engineer materials
US8945567B2 (en) 2009-06-05 2015-02-03 Ablynx N.V. Monovalent, bivalent and trivalent anti human respiratory syncytial virus (HRSV) nanobody constructs for the prevention and/or treatment of respiratory tract infections
AU2010278702C1 (en) 2009-07-31 2016-07-14 Forsyth Dental Infirmary For Children Programming of cells for tolerogenic therapies
US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
US8658434B2 (en) * 2009-10-28 2014-02-25 Biotium, Inc. Fluorescent pyrene compounds
EP2507262A1 (de) 2009-11-30 2012-10-10 Ablynx N.V. Verbesserte gegen hrsv gerichtete aminosäuresequenzen und polypeptide damit zur prävention und/oder behandlung von atemwegsinfektionen
WO2011083141A2 (en) 2010-01-08 2011-07-14 Ablynx Nv Method for generation of immunoglobulin sequences by using lipoprotein particles
DE102010009445A1 (de) 2010-02-25 2011-08-25 Universitätsklinikum Jena, 07743 Expressionsvektor zur Enzym-Expression in Zellen und Verfahren zu dessen Verwendung
EP2542230A4 (de) 2010-03-05 2013-08-28 Harvard College Förderung des anwachsens von skelettmuskel-stammzellen durch gleichzeitige verabreichung von vegf und igf-1
EP2585053A4 (de) 2010-06-25 2014-02-26 Harvard College Gemeinsame freisetzung von stimulations- und hemmfaktoren zur erzeugung von temporär stabilen und räumlich begrenzten zonen
EP3202415B1 (de) 2010-06-30 2021-12-08 Compugen Ltd. Fusionsprotein beinhaltend c1orf32 und eine oder mehrere domänen einer immunoglobulin heavy chain konstanten region zur behandlung von multipler sklerose, rheumatoider arthritis und anderen autoimmunerkrankungen
AU2011289579B2 (en) 2010-08-10 2016-11-17 Ecole Polytechnique Federale De Lausanne Erythrocyte-binding therapeutics
US9517257B2 (en) 2010-08-10 2016-12-13 Ecole Polytechnique Federale De Lausanne (Epfl) Erythrocyte-binding therapeutics
US9850296B2 (en) 2010-08-10 2017-12-26 Ecole Polytechnique Federale De Lausanne (Epfl) Erythrocyte-binding therapeutics
KR20130100290A (ko) 2010-08-30 2013-09-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 협착성 병변을 위한 전단 제어 방출 및 혈전 용해 치료법
EP2616100B1 (de) 2010-09-17 2016-08-31 Compugen Ltd. Zusammensetzungen und verfahren zur behandlung von wirkstoffresistentem multiplem myelom
CA2813751C (en) 2010-10-06 2019-11-12 President And Fellows Of Harvard College Injectable, pore-forming hydrogels for materials-based cell therapies
US9603894B2 (en) 2010-11-08 2017-03-28 President And Fellows Of Harvard College Materials presenting notch signaling molecules to control cell behavior
AU2012219582A1 (en) 2011-02-21 2013-08-22 Fibrostatin S.L. Methods for treating and diagnosing disease
US10647959B2 (en) 2011-04-27 2020-05-12 President And Fellows Of Harvard College Cell-friendly inverse opal hydrogels for cell encapsulation, drug and protein delivery, and functional nanoparticle encapsulation
AU2012249456A1 (en) 2011-04-28 2013-10-31 President And Fellows Of Harvard College Injectable preformed macroscopic 3-dimensional scaffolds for minimally invasive administration
US9675561B2 (en) 2011-04-28 2017-06-13 President And Fellows Of Harvard College Injectable cryogel vaccine devices and methods of use thereof
EP2709450A4 (de) 2011-05-20 2015-04-15 Benjamin Wolozin Nachweis von verbindungen zur dispergierung von tdp-43-einschlüssen
AU2012261848B2 (en) 2011-06-03 2017-06-15 President And Fellows Of Harvard College In situ antigen-generating cancer vaccine
KR20140068087A (ko) 2011-08-23 2014-06-05 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 펩티드 나노입자 및 이의 용도
GB201121565D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Engineering fluorescent proteins for enhanced fret and uses thereof
GB201121570D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Methods for producing engineered fluorescent proteins for enhanced fret, products and uses thereof
EP4108782B1 (de) 2011-12-22 2023-06-07 President and Fellows of Harvard College Zusammensetzungen und verfahren zur analytdetektion
WO2013130808A1 (en) 2012-02-29 2013-09-06 D.E. Shaw Research, Llc Methods for screening voltage gated proteins
CA2870309C (en) 2012-04-16 2024-02-20 President And Fellows Of Harvard College Mesoporous silica compositions for modulating immune responses
JP6388575B2 (ja) 2012-06-06 2018-09-12 アメリカ合衆国 内分泌かく乱物質（ｅｄｃ）を検出およびモニターするためのキット
CN104684584A (zh) 2012-07-02 2015-06-03 费卜卢斯坦丁公司 Gpbp-1的抑制及其治疗用途
US9682123B2 (en) 2013-12-20 2017-06-20 The Trustees Of Columbia University In The City Of New York Methods of treating metabolic disease
SG10202010936RA (en) 2014-02-21 2020-12-30 Ecole Polytecnique Fed De Lausanne Epfl Epfl Tto Glycotargeting therapeutics
US10953101B2 (en) 2014-02-21 2021-03-23 École Polytechnique Fédérale De Lausanne (Epfl) Glycotargeting therapeutics
US10946079B2 (en) 2014-02-21 2021-03-16 Ecole Polytechnique Federale De Lausanne Glycotargeting therapeutics
US10046056B2 (en) 2014-02-21 2018-08-14 École Polytechnique Fédérale De Lausanne (Epfl) Glycotargeting therapeutics
CA2945945C (en) 2014-04-15 2023-10-03 The Regents Of The University Of California Bi-terminal pegylated integrin-binding peptides and methods of use thereof
WO2015168379A2 (en) 2014-04-30 2015-11-05 President And Fellows Of Harvard College Combination vaccine devices and methods of killing cancer cells
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
WO2016007886A1 (en) 2014-07-11 2016-01-14 Northwestern University Yeast-based biosensor
US20170369474A1 (en) 2014-12-05 2017-12-28 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use
CA2969756A1 (en) 2014-12-05 2016-06-09 Aquinnah Pharmaceuticals, Inc. Sulfonamide derivatives, compositions and methods of use in the treatment of neurodegenerative diseases
US11786457B2 (en) 2015-01-30 2023-10-17 President And Fellows Of Harvard College Peritumoral and intratumoral materials for cancer therapy
CN107708756A (zh) 2015-04-10 2018-02-16 哈佛学院院长等 免疫细胞捕获装置及其制备和使用方法
AU2016291846B2 (en) 2015-07-13 2022-05-26 Compugen Ltd. HIDE1 Compositions and Methods
CA2997947A1 (en) 2015-09-09 2017-03-16 The Trustees Of Columbia University In The City Of New York Reduction of er-mam-localized app-c99 and methods of treating alzheimer's disease
CA3001857A1 (en) 2015-10-14 2017-04-20 Aquinnah Pharmaceuticals, Inc. Compounds, compositions and methods of use against stress granules
US20180312534A1 (en) 2015-10-16 2018-11-01 Invictus Oncology Pvt. Ltd. Fluorescent anticancer platinum drugs
EP3411475A4 (de) 2016-02-06 2019-09-11 President and Fellows of Harvard College Rekapitulation der hämatopoietischen nische zur wiederherstellung der immunität
CN115305229A (zh) 2016-07-13 2022-11-08 哈佛学院院长等 抗原呈递细胞模拟支架及其制备和使用方法
US11840569B2 (en) 2016-11-16 2023-12-12 Ablynx N.V. T cell recruiting polypeptides capable of binding CD123 and TCR alpha/beta
EP3638296A1 (de) 2017-06-16 2020-04-22 The University Of Chicago Zusammensetzungen und verfahren zur herbeiführung einer immuntoleranz
WO2019136421A1 (en) * 2018-01-08 2019-07-11 Lucidicor Inc. Fluorescent protein biosensor for detection of kinase activity
WO2019162521A1 (en) 2018-02-26 2019-08-29 Ablynx Nv Improved nucleotide sequences encoding peptide linkers
BR102019017792A2 (pt) 2019-08-27 2021-11-16 Fundação Oswaldo Cruz Receptáculo proteico, método para produção do receptáculo, método de identificação de patógenos ou de diagnóstico de doenças, e, uso do receptáculo
EP4136457A1 (de) 2020-04-17 2023-02-22 Regeneron Pharmaceuticals, Inc. Nachweistests für coronavirusneutralisierende antikörper
CN113265428B (zh) * 2021-06-11 2023-03-14 东北林业大学 一种利用金属硫蛋白构建活细胞内铜变化的检测系统及应用
IL310330A (en) 2021-07-30 2024-03-01 Confo Therapeutics N V Compounds for the treatment of pain, in particular neuropathic pain, and/or other diseases or disorders associated with AT2R and/or AT2R-mediated signaling

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8607563A1 (es) * 1983-10-13 1986-06-01 Univ Georgia Res Found Un metodo para detectar una interaccion ligante
JPS6439990A (en) * 1987-08-05 1989-02-10 Chisso Corp Fused gene by bonding aequorin gene to functional gene
GB8916806D0 (en) * 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US6146826A (en) * 1993-09-10 2000-11-14 The Trustees Of Columbia University In The City Of New York Green fluorescent protein
WO1995021191A1 (en) * 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US5795737A (en) * 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5958713A (en) * 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
AU4483096A (en) * 1995-01-31 1996-08-21 Novo Nordisk A/S A method of detecting biologically active substances
WO1996027027A1 (en) * 1995-03-02 1996-09-06 Rutgers The State University Of New Jersey Improved method for purifying green fluorescent protein
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
US5741657A (en) 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
US6342379B1 (en) 1995-06-07 2002-01-29 The Regents Of The University Of California Detection of transmembrane potentials by optical methods
AU4482996A (en) * 1995-09-22 1997-04-09 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5804387A (en) * 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6027881A (en) * 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5925558A (en) 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US6593135B2 (en) 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
WO1998021355A1 (en) * 1996-11-15 1998-05-22 Life Technologies, Inc. Mutants of green fluorescent protein
US6670449B1 (en) * 1997-02-13 2003-12-30 Memorial Sloan-Kettering Cancer Center Hybrid molecules and their use for optically detecting changes in cellular microenvironments
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6046925A (en) 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
US6008378A (en) 1997-10-21 1999-12-28 The Regents Of The University Of California Synthetic molecules that specifically react with target sequences
US6140132A (en) 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6150176A (en) 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US7060793B2 (en) 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US6469154B1 (en) 1999-05-21 2002-10-22 The Regents Of The University Of California Fluorescent protein indicators
US7212640B2 (en) 1999-11-29 2007-05-01 Bizjak Karl M Variable attack and release system and method
US7015310B2 (en) 2001-03-12 2006-03-21 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation reduction sensitive green fluorescent protein variants

Also Published As

Publication number Publication date
AU4327797A (en) 1998-03-06
US6124128A (en) 2000-09-26
DK0886644T3 (da) 2005-03-14
JP4232992B2 (ja) 2009-03-04
US6403374B1 (en) 2002-06-11
ES2231890T3 (es) 2005-05-16
US20030036178A1 (en) 2003-02-20
US6077707A (en) 2000-06-20
CA2232242C (en) 2008-10-21
EP1508574A3 (de) 2005-11-30
US7560287B2 (en) 2009-07-14
JP2001506842A (ja) 2001-05-29
WO1998006737A1 (en) 1998-02-19
CA2232242A1 (en) 1998-02-19
EP0886644B1 (de) 2004-10-20
US20050079525A1 (en) 2005-04-14
US6780975B2 (en) 2004-08-24
ATE280178T1 (de) 2004-11-15
EP0886644A1 (de) 1998-12-30
EP1508574A2 (de) 2005-02-23
DE69731285T2 (de) 2006-03-09
AU727088B2 (en) 2000-11-30
US6054321A (en) 2000-04-25
EP0886644A4 (de) 2000-01-19

Similar Documents

Publication Publication Date Title
DE69731285D1 (de) Langwellig fluorezierende proteine
PT939767E (pt) Factor viii porcino e seus hibridos
DE69933216D1 (de) Erythropoietin-analog-menschliches serum-albumin fusionsprotein
ATE361365T1 (de) Streptococcus pneumoniae proteine und nukleinsäuren
AU3471697A (en) An improved method for the capillary electrophoresis of nucleic acids, proteins and low molecular charged compounds
ITTO950367A0 (it) Proteine di prrsv ricombinanti, corredi diagnostici e vaccini contenenti tali proteine di prrsv ricombinanti.
NO20016243D0 (no) Anti-alfav-beta3-rekombinante humane antistoffer, nukleinsyrer som koder for de samme og fremgangsmåter foranvendelse
ATE421973T1 (de) Inzektizide proteinen von bacillus thuringiensis
NO2013009I1 (no) Alipogen tiparvovec
EP1712623A3 (de) Selektion von Proteinen unter Verwendung von RNA-protein-fusionen
DE19882430T1 (de) Korrektur von Emissions-Kontamination und Totzeitverlust
DE69534778D1 (en) Cathepsin-02-protease
DE622971T1 (de) Lautsprecheraufbau und Methode zur Assemblierung.
DE69637579D1 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
AU2001263269A1 (en) Long wavelength engineered fluorescent proteins
AU2001238212A1 (en) Modified fluorescent proteins
DK0920501T3 (da) Neuritin, et neurogen
WO2001032882A3 (en) Nucleic acids and proteins from group b streptococcus
DE69728897D1 (de) Saeugetierchemokine
DE69734586D1 (de) Retinoid-metabolisierendes protein
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
ES2165358T3 (es) Utilizaciones de la proteinquinasa de neurofilamento/tau pk40.
AU7846000A (en) Novel human lipase proteins, nucleic acids encoding them, and uses of both of these
EP0964701A4 (de) Leptin-protein aus rindern, die dafür kodierende nukleinsäuresequenz und ihre verwendungsmöglichkeiten
AU7478098A (en) Methods of treatment using nbs-1, antibodies and proteins thereto, and uses of the antibodies

Legal Events

Date Code Title Description
8364 No opposition during term of opposition