DE69738978D1 - Verfahren zum Transport von Fluidproben durch einen mikrofluidischen Kanal - Google Patents

Verfahren zum Transport von Fluidproben durch einen mikrofluidischen Kanal

Info

Publication number
DE69738978D1
DE69738978D1 DE69738978T DE69738978T DE69738978D1 DE 69738978 D1 DE69738978 D1 DE 69738978D1 DE 69738978 T DE69738978 T DE 69738978T DE 69738978 T DE69738978 T DE 69738978T DE 69738978 D1 DE69738978 D1 DE 69738978D1
Authority
DE
Germany
Prior art keywords
materials
microfluidic system
ionic concentration
channel
transported
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738978T
Other languages
English (en)
Inventor
J Wallace Parce
Michael R Knapp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caliper Life Sciences Inc filed Critical Caliper Life Sciences Inc
Priority claimed from PCT/US1997/010963 external-priority patent/WO1998000705A1/en
Application granted granted Critical
Publication of DE69738978D1 publication Critical patent/DE69738978D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B17/00—Pumps characterised by combination with, or adaptation to, specific driving engines or motors
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
    • F04B19/006—Micropumps
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • H—ELECTRICITY
    • H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02N—ELECTRIC MACHINES NOT OTHERWISE PROVIDED FOR
    • H02N11/00—Generators or motors not provided for elsewhere; Alleged perpetua mobilia obtained by electric or magnetic means
    • H02N11/006—Motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B01F33/30351—Micromixers using surface tension to mix, move or hold the fluids using hydrophilic/hydrophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B01F33/30352—Micromixers using surface tension to mix, move or hold the fluids using roughness of the surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
DE69738978T 1996-06-28 1997-06-25 Verfahren zum Transport von Fluidproben durch einen mikrofluidischen Kanal Expired - Lifetime DE69738978D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/671,986 US5779868A (en) 1996-06-28 1996-06-28 Electropipettor and compensation means for electrophoretic bias
US08/760,446 US5880071A (en) 1996-06-28 1996-12-06 Electropipettor and compensation means for electrophoretic bias
PCT/US1997/010963 WO1998000705A1 (en) 1996-06-28 1997-06-25 Electropipettor and compensation means for electrophoretic bias

Publications (1)

Publication Number Publication Date
DE69738978D1 true DE69738978D1 (de) 2008-10-23

Family

ID=24696687

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69738978T Expired - Lifetime DE69738978D1 (de) 1996-06-28 1997-06-25 Verfahren zum Transport von Fluidproben durch einen mikrofluidischen Kanal
DE69739094T Expired - Lifetime DE69739094D1 (de) 1996-06-28 1997-06-27 Elektrokinetische Pipette, sowie mittel zum Kompensieren von elektrophoretischen Scheidungseffekten

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69739094T Expired - Lifetime DE69739094D1 (de) 1996-06-28 1997-06-27 Elektrokinetische Pipette, sowie mittel zum Kompensieren von elektrophoretischen Scheidungseffekten

Country Status (7)

Country Link
US (2) US5779868A (de)
EP (1) EP1524034B1 (de)
KR (1) KR100579763B1 (de)
CN (1) CN101185871A (de)
AT (2) ATE407735T1 (de)
DE (2) DE69738978D1 (de)
ZA (1) ZA975758B (de)

Families Citing this family (323)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US5885470A (en) * 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
EP0909385B1 (de) * 1996-06-28 2008-09-10 Caliper Life Sciences, Inc. Verfahren zum Transport von Fluidproben durch einen mikrofluidischen Kanal
US20060000722A1 (en) * 1996-06-28 2006-01-05 Caliper Life Sciences, Inc. High throughput screening assay systems in microscale fluidic devices
NZ333346A (en) 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US5699157A (en) * 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6447727B1 (en) * 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
US6465257B1 (en) 1996-11-19 2002-10-15 Caliper Technologies Corp. Microfluidic systems
GB9625491D0 (en) * 1996-12-07 1997-01-22 Central Research Lab Ltd Fluid connections
ATE218194T1 (de) * 1996-12-11 2002-06-15 Gesim Ges Fuer Silizium Mikros Mikroejektionspumpe
US5964995A (en) * 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
EP0972082A4 (de) 1997-04-04 2007-04-25 Caliper Life Sciences Inc Biochemische analysatoren, die als geschlossenes system arbeiten
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US7033474B1 (en) 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US5976336A (en) * 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
AU727083B2 (en) * 1997-04-25 2000-11-30 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US6090251A (en) * 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
US6613512B1 (en) 1997-06-09 2003-09-02 Caliper Technologies Corp. Apparatus and method for correcting for variable velocity in microfluidic systems
US5900130A (en) * 1997-06-18 1999-05-04 Alcara Biosciences, Inc. Method for sample injection in microchannel device
US6425972B1 (en) * 1997-06-18 2002-07-30 Calipher Technologies Corp. Methods of manufacturing microfabricated substrates
US6440725B1 (en) 1997-12-24 2002-08-27 Cepheid Integrated fluid manipulation cartridge
US6368871B1 (en) * 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
US5965410A (en) * 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
AU746098B2 (en) * 1997-09-02 2002-04-18 Caliper Life Sciences, Inc. Microfluidic system with electrofluidic and electrothermal controls
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7214298B2 (en) * 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6803019B1 (en) * 1997-10-15 2004-10-12 Aclara Biosciences, Inc. Laminate microstructure device and method for making same
US6174675B1 (en) 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6893877B2 (en) * 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US6857449B1 (en) 1998-01-20 2005-02-22 Caliper Life Sciences, Inc. Multi-layer microfluidic devices
US6167910B1 (en) 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US7497994B2 (en) * 1998-02-24 2009-03-03 Khushroo Gandhi Microfluidic devices and systems incorporating cover layers
US6123798A (en) * 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
EP1084391A4 (de) * 1998-06-08 2006-06-14 Caliper Life Sciences Inc Mikrofluidische geräte, systeme und verfahren zur durchführung integrierter reaktionen und trennungen
US6459080B1 (en) * 1998-06-12 2002-10-01 Agilent Technologies, Inc. Miniaturized device for separating the constituents of a sample and delivering the constituents of the separated sample to a mass spectrometer
US6540896B1 (en) * 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
CN100435900C (zh) * 1998-09-17 2008-11-26 阿德文生物科学公司 液相色谱系统，化学分离装置及质谱分析装置和方法
US6498005B1 (en) 1998-09-30 2002-12-24 Caliper Technologies Corp. Homogeneous assay methods
US20020192719A1 (en) * 1998-09-30 2002-12-19 Caliper Technologies Corp. Homogeneous assay methods
US6605472B1 (en) * 1998-10-09 2003-08-12 The Governors Of The University Of Alberta Microfluidic devices connected to glass capillaries with minimal dead volume
US6149787A (en) * 1998-10-14 2000-11-21 Caliper Technologies Corp. External material accession systems and methods
US6086740A (en) * 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US7914994B2 (en) * 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
US6150119A (en) 1999-01-19 2000-11-21 Caliper Technologies Corp. Optimized high-throughput analytical system
US6416642B1 (en) * 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US20020019059A1 (en) * 1999-01-28 2002-02-14 Calvin Y.H. Chow Devices, systems and methods for time domain multiplexing of reagents
US6475364B1 (en) 1999-02-02 2002-11-05 Caliper Technologies Corp. Methods, devices and systems for characterizing proteins
EP1163052B1 (de) * 1999-02-23 2010-06-02 Caliper Life Sciences, Inc. Manipulation von mikroteilchen in mikrofluiden systemen
US6633031B1 (en) 1999-03-02 2003-10-14 Advion Biosciences, Inc. Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6148508A (en) * 1999-03-12 2000-11-21 Caliper Technologies Corp. Method of making a capillary for electrokinetic transport of materials
US6296702B1 (en) * 1999-03-15 2001-10-02 Pe Corporation (Ny) Apparatus and method for spotting a substrate
US6500323B1 (en) 1999-03-26 2002-12-31 Caliper Technologies Corp. Methods and software for designing microfluidic devices
JP2000283960A (ja) * 1999-03-31 2000-10-13 Shimadzu Corp マイクロチップ電気泳動装置
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6193647B1 (en) 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
US6322683B1 (en) 1999-04-14 2001-11-27 Caliper Technologies Corp. Alignment of multicomponent microfabricated structures
US6605475B1 (en) 1999-04-16 2003-08-12 Perspective Biosystems, Inc. Apparatus and method for sample delivery
US6375817B1 (en) * 1999-04-16 2002-04-23 Perseptive Biosystems, Inc. Apparatus and methods for sample analysis
US6458259B1 (en) 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
AU770678B2 (en) 1999-05-17 2004-02-26 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US6472141B2 (en) * 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US8815521B2 (en) 2000-05-30 2014-08-26 Cepheid Apparatus and method for cell disruption
US9073053B2 (en) 1999-05-28 2015-07-07 Cepheid Apparatus and method for cell disruption
US20040200909A1 (en) * 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
ATE278771T1 (de) 1999-05-28 2004-10-15 Cepheid Vorrichtung und verfahren zur analyse flüssiger proben
US6406605B1 (en) 1999-06-01 2002-06-18 Ysi Incorporated Electroosmotic flow controlled microfluidic devices
US6649358B1 (en) 1999-06-01 2003-11-18 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
US6878540B2 (en) * 1999-06-25 2005-04-12 Cepheid Device for lysing cells, spores, or microorganisms
IL147302A0 (en) 1999-06-28 2002-08-14 California Inst Of Techn Microfabricated elastomeric valve and pump systems
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6524456B1 (en) * 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
AU6580700A (en) * 1999-08-13 2001-03-13 Cartesian Technologies, Inc. Apparatus for liquid sample handling
US6495104B1 (en) * 1999-08-19 2002-12-17 Caliper Technologies Corp. Indicator components for microfluidic systems
JP4119649B2 (ja) * 1999-08-25 2008-07-16 カリパー・ライフ・サイエンシズ・インコーポレーテッド 単一真空源を有する高スループットシステムにおける稀釈
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
WO2001017797A1 (en) 1999-09-10 2001-03-15 Caliper Technologies Corp. Microfabrication methods and devices
EP1222257A4 (de) * 1999-10-08 2007-12-12 Caliper Life Sciences Inc Verwendung von nernstpotenzial-empfindlichen farbstoffen bei der messung von transmembranpotenzialen
US6386014B1 (en) 1999-11-18 2002-05-14 Eagle Research Corporation Energy measurement device for flowing gas using microminiature gas chromatograph
JP5057318B2 (ja) 1999-12-30 2012-10-24 アドビオン インコーポレイテッド 多重電気噴霧装置、システム、および方法
AU2610201A (en) 2000-01-06 2001-07-16 Caliper Technologies Corporation Ultra high throughput sampling and analysis systems and methods
US6468761B2 (en) 2000-01-07 2002-10-22 Caliper Technologies, Corp. Microfluidic in-line labeling method for continuous-flow protease inhibition analysis
US7037416B2 (en) * 2000-01-14 2006-05-02 Caliper Life Sciences, Inc. Method for monitoring flow rate using fluorescent markers
EP1248949B1 (de) 2000-01-18 2013-05-22 Advion, Inc. Elektrosprayvorrichtung mit array von trennsäulen sowie verfahren zur trennung von fluidproben
US6589729B2 (en) * 2000-02-04 2003-07-08 Caliper Technologies Corp. Methods, devices, and systems for monitoring time dependent reactions
US20040108207A1 (en) * 2000-02-11 2004-06-10 Aclara Biosciences, Inc. Injection and separation system and method employing transient isotachophoretic stacking
US6685813B2 (en) * 2000-02-11 2004-02-03 Aclara Biosciences, Inc. Tandem isotachophoresis/zone electrophoresis method and system
AU2001240040A1 (en) * 2000-03-03 2001-09-17 California Institute Of Technology Combinatorial array for nucleic acid analysis
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
US6358387B1 (en) * 2000-03-27 2002-03-19 Caliper Technologies Corporation Ultra high throughput microfluidic analytical systems and methods
US6481453B1 (en) * 2000-04-14 2002-11-19 Nanostream, Inc. Microfluidic branch metering systems and methods
US6733645B1 (en) 2000-04-18 2004-05-11 Caliper Technologies Corp. Total analyte quantitation
EP1279071B1 (de) 2000-05-03 2011-03-23 Caliper Life Sciences, Inc. Herstellungsprozesse für substrate mit mehreren tiefen
CA2406718A1 (en) 2000-05-11 2001-11-15 Caliper Technologies Corp. Microfluidic devices and methods to regulate hydrodynamic and electrical resistance utilizing bulk viscosity enhancers
EP1297179B1 (de) * 2000-05-12 2008-09-24 Caliper Life Sciences, Inc. Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
GB2362713A (en) * 2000-05-26 2001-11-28 Casect Ltd Sampling system for gas
US7351376B1 (en) * 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6829753B2 (en) 2000-06-27 2004-12-07 Fluidigm Corporation Microfluidic design automation method and system
US20070119711A1 (en) * 2000-08-02 2007-05-31 Caliper Life Sciences, Inc. High throughput separations based analysis systems and methods
CA2412394A1 (en) * 2000-08-02 2002-02-07 Walter Ausserer High throughput separations based analysis systems
AU8470001A (en) * 2000-08-03 2002-02-18 Caliper Techn Corp Methods and devices for high throughput fluid delivery
US20020142618A1 (en) 2000-08-04 2002-10-03 Caliper Technologies Corp. Control of operation conditions within fluidic systems
DE60140553D1 (de) * 2000-09-14 2009-12-31 Caliper Life Sciences Inc MIKROFLUIDISCHE VORRICHTUNGEN UND METHODEN UM TEMPERATUR-VERMITTELTE REAKTIONEN DURCHZUFüHREN
US6706162B1 (en) * 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
EP1336097A4 (de) 2000-10-13 2006-02-01 Fluidigm Corp Probeninjektionssystem auf der basis einer mikrofluidischen einrichtung für analytische einrichtungen
US20050011761A1 (en) * 2000-10-31 2005-01-20 Caliper Technologies Corp. Microfluidic methods, devices and systems for in situ material concentration
US20030057092A1 (en) * 2000-10-31 2003-03-27 Caliper Technologies Corp. Microfluidic methods, devices and systems for in situ material concentration
US20090118139A1 (en) 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
US6681788B2 (en) 2001-01-29 2004-01-27 Caliper Technologies Corp. Non-mechanical valves for fluidic systems
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
DE60234572D1 (de) * 2001-02-15 2010-01-14 Caliper Life Sciences Inc Mikrofluidische systeme mit verbesserten detektionssystemen
US6720148B1 (en) 2001-02-22 2004-04-13 Caliper Life Sciences, Inc. Methods and systems for identifying nucleotides by primer extension
US7867776B2 (en) * 2001-03-02 2011-01-11 Caliper Life Sciences, Inc. Priming module for microfluidic chips
US20050196785A1 (en) * 2001-03-05 2005-09-08 California Institute Of Technology Combinational array for nucleic acid analysis
US7150999B1 (en) 2001-03-09 2006-12-19 Califer Life Sciences, Inc. Process for filling microfluidic channels
CA2441366A1 (en) 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20060029955A1 (en) * 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use
US20050196746A1 (en) * 2001-03-24 2005-09-08 Jia Xu High-density ion transport measurement biochip devices and methods
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7192557B2 (en) * 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US20020164816A1 (en) * 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6752922B2 (en) * 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US6805841B2 (en) 2001-05-09 2004-10-19 The Provost Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin Liquid pumping system
US7723123B1 (en) * 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US20020187564A1 (en) * 2001-06-08 2002-12-12 Caliper Technologies Corp. Microfluidic library analysis
US6977163B1 (en) 2001-06-13 2005-12-20 Caliper Life Sciences, Inc. Methods and systems for performing multiple reactions by interfacial mixing
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US20030015425A1 (en) * 2001-06-20 2003-01-23 Coventor Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
JP2005514187A (ja) * 2001-06-20 2005-05-19 サイトノーム インコーポレーテッド 流体をマイクロ流体システムと相互接続するための仮想壁流体相互接続ポートを含むマイクロ流体システム
US20020197733A1 (en) * 2001-06-20 2002-12-26 Coventor, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US20030077570A1 (en) * 2001-09-20 2003-04-24 Coventor, Inc. Small molecule substrate based enzyme activity assays
US7211442B2 (en) * 2001-06-20 2007-05-01 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7077152B2 (en) * 2001-07-07 2006-07-18 Nanostream, Inc. Microfluidic metering systems and methods
ATE465811T1 (de) * 2001-07-13 2010-05-15 Caliper Life Sciences Inc Methode zur trennung von komponenten eines gemisches
US6825127B2 (en) 2001-07-24 2004-11-30 Zarlink Semiconductor Inc. Micro-fluidic devices
US20060062696A1 (en) * 2001-07-27 2006-03-23 Caliper Life Sciences, Inc. Optimized high throughput analytical systems
US7060171B1 (en) 2001-07-31 2006-06-13 Caliper Life Sciences, Inc. Methods and systems for reducing background signal in assays
WO2003016547A2 (en) * 2001-08-13 2003-02-27 Vanderbilt University Distribution of solutions across a surface
US6726820B1 (en) * 2001-09-19 2004-04-27 Applera Corporation Method of separating biomolecule-containing samples with a microdevice with integrated memory
ATE487938T1 (de) * 2001-09-07 2010-11-15 Life Technologies Corp Konzentration und aufreinigung von nukleinsäureproben
US20060157349A1 (en) * 2002-09-09 2006-07-20 Applera Corporation Concentration and Cleanup of Nucleic Acid Samples
US6942018B2 (en) * 2001-09-28 2005-09-13 The Board Of Trustees Of The Leland Stanford Junior University Electroosmotic microchannel cooling system
US7134486B2 (en) * 2001-09-28 2006-11-14 The Board Of Trustees Of The Leeland Stanford Junior University Control of electrolysis gases in electroosmotic pump systems
US20030062833A1 (en) * 2001-10-03 2003-04-03 Wen-Yen Tai Mini-type decorative bulb capable of emitting light through entire circumferential face
US6607362B2 (en) 2001-10-11 2003-08-19 Agilent Technologies, Inc. Micro paddle wheel pump for precise pumping, mixing, dispensing, and valving of blood and reagents
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
US6750661B2 (en) * 2001-11-13 2004-06-15 Caliper Life Sciences, Inc. Method and apparatus for controllably effecting samples using two signals
US7247274B1 (en) 2001-11-13 2007-07-24 Caliper Technologies Corp. Prevention of precipitate blockage in microfluidic channels
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
IE20020619A1 (en) * 2001-12-31 2003-07-23 Provost Fellows And Scholors O An assay assembly
CA2477702A1 (en) * 2002-03-05 2003-09-18 Caliper Life Sciences, Inc. Mixed mode microfluidic systems abstract of the disclosure
US7195986B1 (en) 2002-03-08 2007-03-27 Caliper Life Sciences, Inc. Microfluidic device with controlled substrate conductivity
US7252928B1 (en) 2002-03-12 2007-08-07 Caliper Life Sciences, Inc. Methods for prevention of surface adsorption of biological materials to capillary walls in microchannels
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US7419784B2 (en) * 2002-04-02 2008-09-02 Dubrow Robert S Methods, systems and apparatus for separation and isolation of one or more sample components of a sample biological material
US8241883B2 (en) * 2002-04-24 2012-08-14 Caliper Life Sciences, Inc. High throughput mobility shift
US6621076B1 (en) 2002-04-30 2003-09-16 Agilent Technologies, Inc. Flexible assembly for transporting sample fluids into a mass spectrometer
EP1501924A4 (de) * 2002-05-04 2006-05-24 Aviva Biosciences Corp Vorrichtung mit strukturen nachweisendem ionentransport und verwendungsverfahren
US20030224531A1 (en) * 2002-05-29 2003-12-04 Brennen Reid A. Microplate with an integrated microfluidic system for parallel processing minute volumes of fluids
US7161356B1 (en) 2002-06-05 2007-01-09 Caliper Life Sciences, Inc. Voltage/current testing equipment for microfluidic devices
US20050238506A1 (en) * 2002-06-21 2005-10-27 The Charles Stark Draper Laboratory, Inc. Electromagnetically-actuated microfluidic flow regulators and related applications
US7867193B2 (en) * 2004-01-29 2011-01-11 The Charles Stark Draper Laboratory, Inc. Drug delivery apparatus
US20060086309A1 (en) * 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
US20040053315A1 (en) * 2002-08-12 2004-03-18 Caliper Technologies Corp. Methods and systems for monitoring molecular interactions
AU2003304163A1 (en) 2002-08-21 2005-01-21 Shell Oil Company Method for measuring fluid chemistry in drilling and production operations
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP2298448A3 (de) 2002-09-25 2012-05-30 California Institute of Technology Hochintegriertes mikrofluidisches Netzwerk
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US7932098B2 (en) * 2002-10-31 2011-04-26 Hewlett-Packard Development Company, L.P. Microfluidic system utilizing thin-film layers to route fluid
US20060043284A1 (en) * 2002-11-29 2006-03-02 Nec Corporation Micro chip, liquid feeding method using the micro chip, and mass analyzing system
WO2004051247A2 (en) * 2002-12-02 2004-06-17 Protasis Corporation Electrophoretic device comprising separation chamber, non-uniform electrode chamber, and a porous membrane between them
US7087444B2 (en) * 2002-12-16 2006-08-08 Palo Alto Research Center Incorporated Method for integration of microelectronic components with microfluidic devices
AU2003302770B2 (en) * 2002-12-20 2007-07-12 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
US6725882B1 (en) * 2003-01-03 2004-04-27 Industrial Technology Research Institute Configurable micro flowguide device
US20060124459A1 (en) * 2003-01-15 2006-06-15 Protassis Corporation Devices and methods for focusing analytes in an electric field gradient II
US7355010B2 (en) 2003-01-30 2008-04-08 Bellbrook Labs, Llc Assay method for group transfer reactions
US8088897B2 (en) * 2003-01-30 2012-01-03 BellBrook Labs, Inc. Assay method for group transfer reactions
WO2004068115A2 (en) * 2003-01-30 2004-08-12 Bellbrook Labs, Llc Assay method for group transfer reactions
SE0300454D0 (sv) * 2003-02-19 2003-02-19 Aamic Ab Nozzles for electrospray ionization and methods of fabricating them
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7476363B2 (en) * 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
EP2340890B1 (de) 2003-04-03 2016-10-19 Fluidigm Corporation Verfahren zur Durchführung von Digitaler PCR
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7007710B2 (en) * 2003-04-21 2006-03-07 Predicant Biosciences, Inc. Microfluidic devices and methods
WO2004113898A1 (en) * 2003-05-19 2004-12-29 Protasis Corporation Electrophoresis devices and methods for focusing charged analytes
US20070151615A1 (en) * 2003-05-19 2007-07-05 Protasis Corporation Bulk fluid flow gate
WO2004104555A2 (en) * 2003-05-19 2004-12-02 Protasis Corporation Fluid logic device
US7572409B2 (en) * 2003-05-23 2009-08-11 Applied Biosystems, Llc Ionic liquid apparatus and method for biological samples
US7578916B2 (en) * 2004-05-25 2009-08-25 Applied Biosystems, Llc Emulsions of ionic liquids
US7316543B2 (en) * 2003-05-30 2008-01-08 The Board Of Trustees Of The Leland Stanford Junior University Electroosmotic micropump with planar features
US20040253642A1 (en) * 2003-06-06 2004-12-16 Grant Zimmermann System and method for multidimensional evaluation of combinations of compositions
EP2402089A1 (de) 2003-07-31 2012-01-04 Handylab, Inc. Verarbeitung partikelhaltiger Proben
US7231839B2 (en) * 2003-08-11 2007-06-19 The Board Of Trustees Of The Leland Stanford Junior University Electroosmotic micropumps with applications to fluid dispensing and field sampling
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7407799B2 (en) 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
US7867194B2 (en) 2004-01-29 2011-01-11 The Charles Stark Draper Laboratory, Inc. Drug delivery apparatus
DE602004014819D1 (de) * 2004-02-02 2008-08-14 Agilent Technologies Inc Reduktion geladener partikel bei der analytbearbeitung
US7187286B2 (en) 2004-03-19 2007-03-06 Applera Corporation Methods and systems for using RFID in biological field
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7402229B2 (en) * 2004-03-31 2008-07-22 Intel Corporation Fabrication and use of semipermeable membranes and gels for the control of electrolysis in a microfluidic device
US7282127B2 (en) * 2004-04-13 2007-10-16 East Carolina Microcapillary devices using high dielectric constant materials and related methods
ES2553097T3 (es) 2004-05-03 2015-12-04 Handylab, Inc. Procesamiento de muestras que contienen polinucleótidos
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1778877B1 (de) 2004-07-28 2009-10-21 Canon U.S. Life Sciences, Inc. Verfahren zur überwachung genomischer dna von organismen
US20060070880A1 (en) * 2004-08-31 2006-04-06 Applera Corporation Methods and apparatus for manipulating separation media
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1871903B1 (de) * 2005-02-18 2011-12-21 Canon U.S. Life Sciences, Inc. Vorrichtung und verfahren zum identifizieren genomischer dna von organismen
US20060246493A1 (en) 2005-04-04 2006-11-02 Caliper Life Sciences, Inc. Method and apparatus for use in temperature controlled processing of microfluidic samples
US8206974B2 (en) * 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
KR100679071B1 (ko) * 2005-06-28 2007-02-05 삼성전기주식회사 유체 제어 장치
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2001990B1 (de) 2006-03-24 2016-06-29 Handylab, Inc. Integriertes system zur verarbeitung von mikrofluidischen proben und verwendungsverfahren
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (de) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008094672A2 (en) * 2007-01-31 2008-08-07 Charles Stark Draper Laboratory, Inc. Membrane-based fluid control in microfluidic devices
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US7799656B2 (en) * 2007-03-15 2010-09-21 Dalsa Semiconductor Inc. Microchannels for BioMEMS devices
AU2008236691B2 (en) * 2007-04-04 2014-08-07 Ande Corporation Integrated nucleic acid analysis
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8381169B2 (en) * 2007-10-30 2013-02-19 International Business Machines Corporation Extending unified process and method content to include dynamic and collaborative content
BRPI0819296A2 (pt) * 2007-11-22 2015-05-12 Koninkl Philips Electronics Nv Cartucho de sensor, sensor, métodos para fabricar um cartucho de sensor e para determinar frações alvo em uma amostra de fluido, uso de um cartucho de sensor, dispositivo descartável, e, dispositivo leitor
KR100944047B1 (ko) * 2008-04-01 2010-02-24 한국표준과학연구원 Ｅｓｉ 인터페이스용 도전 결합장치
US10005082B2 (en) 2008-04-11 2018-06-26 Incyto Co., Ltd. Microfluidic circuit element comprising microfluidic channel with nano interstices and fabrication method thereof
KR100998535B1 (ko) * 2008-04-11 2010-12-07 인싸이토 주식회사 나노틈새를 가지는 미세유체 채널이 구비된 미세유체회로소자 및 이의 제조 방법
US9017946B2 (en) 2008-06-23 2015-04-28 Canon U.S. Life Sciences, Inc. Systems and methods for monitoring the amplification of DNA
US8122901B2 (en) 2008-06-30 2012-02-28 Canon U.S. Life Sciences, Inc. System and method for microfluidic flow control
US20090325159A1 (en) * 2008-06-30 2009-12-31 Canon U.S. Life Sciences, Inc. System and method to prevent cross-contamination in assays performed in a microfluidic channel
FR2933315B1 (fr) * 2008-07-07 2012-02-10 Commissariat Energie Atomique Dispositif microfluidique de deplacement de liquide
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
WO2010025302A2 (en) 2008-08-27 2010-03-04 Life Technologies Corporation Apparatus for and method of processing biological samples
US11235323B2 (en) 2008-08-27 2022-02-01 Life Technologies Corporation Apparatus for and method of processing biological samples
US9157550B2 (en) * 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
US7927904B2 (en) 2009-01-05 2011-04-19 Dalsa Semiconductor Inc. Method of making BIOMEMS devices
EP3415235A1 (de) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation von mikrofluidiktröpfchen
GB2473868A (en) 2009-09-28 2011-03-30 Invitrogen Dynal As Apparatus and method of automated processing of biological samples
EP2437887B1 (de) 2009-06-04 2016-05-11 Lockheed Martin Corporation Mikrofluidischer chip für dna analyse von mehrfachen proben
JP2012529908A (ja) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド 法医学的ｄｎａの定量化のための改善された方法
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
JP6162047B2 (ja) 2011-02-02 2017-07-12 ザ チャールズ スターク ドレイパー ラボラトリー インク 薬物送達装置
EP3412778A1 (de) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Verfahren zur bildung gemischter tröpfchen
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2761305B1 (de) 2011-09-30 2017-08-16 Becton, Dickinson and Company Vereinheitlichter reagenzienstreifen
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
GB2521826B (en) * 2013-12-18 2017-11-08 Ge Oil & Gas Uk Ltd Multiple chemical supply line
EP3090063B1 (de) 2013-12-31 2019-11-06 Bio-Rad Laboratories, Inc. Verfahren zur detektion von latentem retrovirus
GB2525633A (en) * 2014-04-30 2015-11-04 Univ Southampton Methods and apparatus for introducing a sample into a separation channel for electrophoresis
WO2016161385A1 (en) * 2015-04-02 2016-10-06 Cepheid Fluidic bridge device and sample processing methods
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
KR20180101350A (ko) 2015-11-30 2018-09-12 인타바이오 인코퍼레이티드 샘플 특성화를 위한 장치 및 방법
CN105729642B (zh) * 2016-04-26 2018-05-04 公安部第一研究所 一种保障石英毛细管阵列切割过程中通道导通的方法和装置
CN107603874B (zh) * 2017-09-12 2024-04-05 深圳市尚维高科有限公司 微流控pcr检测系统
CN111971112A (zh) 2018-01-29 2020-11-20 因塔生物公司 用于样品表征的装置、方法和试剂盒
KR20200115579A (ko) 2018-01-30 2020-10-07 라이프 테크놀로지스 코포레이션 스마트 분자 분석 시스템의 워크플로우에서 사용하기 위한 기기, 장치 및 소모품
DE102018206463A1 (de) * 2018-04-26 2019-10-31 Robert Bosch Gmbh Verfahren zum Verdünnen, Mischen und/oder Aliquotieren von zwei Flüssigkeiten in einem mikrofluidisches System
KR20210015954A (ko) 2018-05-31 2021-02-10 인타바이오 인코퍼레이티드 질량분광법과 인터페이스로 연결된 미소유체 시스템을 위한 소프트웨어
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies
CN114180513A (zh) * 2021-12-08 2022-03-15 三沙供电局有限责任公司 一种半自动注入油料的装置

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5164598A (en) * 1985-08-05 1992-11-17 Biotrack Capillary flow device
US4963498A (en) * 1985-08-05 1990-10-16 Biotrack Capillary flow device
US5144139A (en) * 1985-08-05 1992-09-01 Biotrack, Inc. Capillary flow device
US5140161A (en) * 1985-08-05 1992-08-18 Biotrack Capillary flow device
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5126022A (en) * 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
CH679952A5 (de) * 1990-03-20 1992-05-15 Ciba Geigy Ag
JPH04160356A (ja) * 1990-10-24 1992-06-03 Shimadzu Corp キャピラリー電気泳動装置の試料注入装置
US5089099A (en) * 1990-11-30 1992-02-18 Varian Associates, Inc. Field amplified polarity switching sample injection in capillary zone electrophoresis
US5192405A (en) * 1991-01-11 1993-03-09 Millipore Corporation Process for effecting high efficiency separations by capillary electrophoresis
US5270183A (en) * 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5358612A (en) * 1991-09-24 1994-10-25 The Dow Chemical Company Electrophoresis with chemically suppressed detection
US5116471A (en) * 1991-10-04 1992-05-26 Varian Associates, Inc. System and method for improving sample concentration in capillary electrophoresis
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5370842A (en) * 1991-11-29 1994-12-06 Canon Kabushiki Kaisha Sample measuring device and sample measuring system
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5639423A (en) * 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5302264A (en) * 1992-09-02 1994-04-12 Scientronix, Inc. Capillary eletrophoresis method and apparatus
US5282942A (en) * 1993-01-22 1994-02-01 Beckman Instruments, Inc. Methods and apparatus for separating and mobilizing solutes in a solute mixture
US5415747A (en) * 1993-08-16 1995-05-16 Hewlett-Packard Company Capillary electrophoresis using zwitterion-coated capillary tubes
US5536382A (en) * 1994-05-23 1996-07-16 Advanced Molecular Systems, Inc. Capillary electrophoresis assay method useful for the determination of constituents of a clinical sample
US5700695A (en) * 1994-06-30 1997-12-23 Zia Yassinzadeh Sample collection and manipulation method
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5571410A (en) * 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
DE59600820D1 (de) * 1995-02-01 1998-12-24 Rossendorf Forschzent Elektrisch steuerbare Mikro-Pipette
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5630925A (en) * 1995-07-13 1997-05-20 Beckman Instruments, Inc. Capillary electrophoresis using a conductive capillary tube
US6126899A (en) * 1996-04-03 2000-10-03 The Perkins-Elmer Corporation Device for multiple analyte detection
US6001307A (en) * 1996-04-26 1999-12-14 Kyoto Daiichi Kagaku Co., Ltd. Device for analyzing a sample

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US5779868A (en) 1998-07-14
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KR100579763B1 (ko) 2006-05-15
DE69739094D1 (de) 2008-12-18
CN101185871A (zh) 2008-05-28
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EP1524034A2 (de) 2005-04-20
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