DE69808060T2 - Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen - Google Patents

Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen

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Publication number
DE69808060T2
DE69808060T2 DE69808060T DE69808060T DE69808060T2 DE 69808060 T2 DE69808060 T2 DE 69808060T2 DE 69808060 T DE69808060 T DE 69808060T DE 69808060 T DE69808060 T DE 69808060T DE 69808060 T2 DE69808060 T2 DE 69808060T2
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DE
Germany
Prior art keywords
expression
reporter
library
phenotype
identifying nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69808060T
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English (en)
Other versions
DE69808060D1 (de
Inventor
Alexander Kamb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deltagen Proteomics Inc
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Deltagen Proteomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltagen Proteomics Inc filed Critical Deltagen Proteomics Inc
Application granted granted Critical
Publication of DE69808060D1 publication Critical patent/DE69808060D1/de
Publication of DE69808060T2 publication Critical patent/DE69808060T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
DE69808060T 1997-03-04 1998-02-27 Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen Expired - Fee Related DE69808060T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/812,994 US5955275A (en) 1997-02-14 1997-03-04 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
PCT/US1998/004376 WO1998039483A1 (en) 1997-03-04 1998-02-27 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes

Publications (2)

Publication Number Publication Date
DE69808060D1 DE69808060D1 (de) 2002-10-24
DE69808060T2 true DE69808060T2 (de) 2003-04-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE69808060T Expired - Fee Related DE69808060T2 (de) 1997-03-04 1998-02-27 Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen

Country Status (11)

Country Link
US (3) US5955275A (de)
EP (1) EP0973943B1 (de)
JP (1) JP2001514510A (de)
AT (1) ATE224457T1 (de)
AU (1) AU745827B2 (de)
CA (1) CA2283261C (de)
DE (1) DE69808060T2 (de)
DK (1) DK0973943T3 (de)
IL (1) IL131583A0 (de)
NO (1) NO994290L (de)
WO (1) WO1998039483A1 (de)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0877752T3 (da) * 1996-01-23 2003-09-15 Univ Leland Stanford Junior Fremgangsmåder til screening af transdominante effektorpeptider og RNA molekyler
US6365344B1 (en) * 1996-01-23 2002-04-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and RNA molecules
US5955275A (en) * 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US20040219569A1 (en) * 1999-07-06 2004-11-04 Fruma Yehiely Gene identification method
US20020081688A1 (en) * 1997-03-04 2002-06-27 Kamb Carl Alexander Retinoid pathway assays, and compositions therefrom
US20020086386A1 (en) * 1997-03-04 2002-07-04 Kamb Carl Alexander B-catenin assays, and compositions therefrom
JPH10260223A (ja) * 1997-03-19 1998-09-29 Fujitsu Ltd 半導体検査装置及びこれを用いた検査方法
JP2001513995A (ja) * 1997-08-29 2001-09-11 セレクティブ ジェネティックス， インコーポレイテッド 遺伝子送達のためのインターナリゼーションリガンドを選択するためのファージディスプレイを使用する方法
US6451527B1 (en) 1997-08-29 2002-09-17 Selective Genetics, Inc. Methods using genetic package display for selecting internalizing ligands for gene delivery
US6589730B1 (en) * 1997-08-29 2003-07-08 Selective Genetics, Inc. Methods for identifying protein-protein interactions by selective transduction
AU1582799A (en) * 1997-11-07 1999-05-31 Iconix Pharmaceuticals, Inc. Surrogate genetics target characterization method
US7037648B1 (en) * 1997-11-07 2006-05-02 John Hopkins University Somatic transfer of modified genes to predict drug effects
US6228579B1 (en) * 1997-11-14 2001-05-08 San Diego State University Foundation Method for identifying microbial proliferation genes
GB9726431D0 (en) * 1997-12-15 1998-02-11 Dower Steven Expression cloning and single cell detection of phenotype
US8334238B2 (en) * 1998-04-17 2012-12-18 Rigel, Inc. Multiparameter FACS assays to detect alterations in cellular parameters and to screen small molecule libraries
US6897031B1 (en) * 1998-04-17 2005-05-24 Rigel Pharmaceuticals, Inc. Multiparameter FACS assays to detect alterations in exocytosis
US6472151B1 (en) * 1998-08-19 2002-10-29 Bristol-Myers Squibb Company Method of screening for compounds that modulate the activity of a molecular target
US6936421B2 (en) 1998-10-08 2005-08-30 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US7297482B2 (en) 1998-10-08 2007-11-20 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
IL142427A0 (en) * 1998-11-17 2002-03-10 Arcaris Methods for validating polypeptide targets that correlate to cellular phenotypes
WO2000039346A1 (en) * 1998-12-31 2000-07-06 Iconix Pharmaceuticals, Inc. Method for generating a pathway reporter system
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
IL145055A0 (en) * 1999-03-12 2002-06-30 Gpc Biotech Inc Methods and reagents for identifying synthetic genetic elements
US7803765B2 (en) * 1999-05-05 2010-09-28 Phylogica Limited Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom
CA2721199A1 (en) * 1999-05-05 2000-11-16 Phylogica Limited Isolating biological modulators from biodiverse gene fragment libraries
US7270969B2 (en) * 1999-05-05 2007-09-18 Phylogica Limited Methods of constructing and screening diverse expression libraries
US6589738B1 (en) * 1999-11-09 2003-07-08 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
CA2394805A1 (en) * 1999-12-16 2001-06-21 Iconix Pharmaceuticals, Inc Random domain mapping
JP2003521934A (ja) 2000-02-09 2003-07-22 ジェンベク、インコーポレイティッド ウイルスベクターライブラリーの製造および使用方法
US6582899B1 (en) * 2000-02-15 2003-06-24 Deltagen Proteomics, Inc. Methods for identifying agents that cause a lethal phenotype, and agents thereof
AU4250901A (en) 2000-03-24 2001-10-03 Micromet Ag Mrna amplification
WO2001075178A2 (en) * 2000-04-04 2001-10-11 Enanta Pharmaceuticals, Inc. Methods for identifying peptide aptamers capable of altering a cell phenotype
EP1301612A2 (de) * 2000-05-31 2003-04-16 Genvec, Inc. Verfahren und zusammenfassung zur zielrichtung von adenoviralen vektoren
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US6410246B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
US6410271B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Generation of highly diverse library of expression vectors via homologous recombination in yeast
CA2429515A1 (en) * 2000-11-17 2002-05-23 Deltagen Proteomics, Inc. Retinoid pathway assays, and compositions therefrom
WO2002055697A2 (en) * 2000-11-27 2002-07-18 Deltagen Proteomics Inc Human rhinovirus assays, and compositions comprising anti-rhinoviral perturbagens
JP2004524025A (ja) * 2001-02-05 2004-08-12 シェン クアン パン 活性酸素種のスカベンジャーを特定する方法及びキット
US20030027175A1 (en) * 2001-02-13 2003-02-06 Gregory Stephanopoulos Dynamic whole genome screening methodology and systems
WO2002068630A1 (en) * 2001-02-22 2002-09-06 Praecis Pharmaceuticals Incorporated Methods for identifying peptides which modulate a biological process
WO2002070662A2 (en) 2001-03-02 2002-09-12 Gpc Biotech Ag Three hybrid assay system
WO2002072783A2 (en) * 2001-03-12 2002-09-19 Irm, Llc Identification of cellular targets for biologically active molecules
AU2002252370A1 (en) * 2001-03-12 2002-09-24 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
AU2002253995A1 (en) * 2001-03-29 2002-10-15 Hybrigen, Inc. Improved hybrid gene libraries and uses thereof
US7026123B1 (en) 2001-08-29 2006-04-11 Pioneer Hi-Bred International, Inc. UTR tag assay for gene function discovery
US20030134329A1 (en) * 2001-10-09 2003-07-17 Thea Norman Cross-species bioactive peptides
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US20030170694A1 (en) * 2001-12-21 2003-09-11 Daniel Wall Stabilized nucleic acids in gene and drug discovery and methods of use
US20030143547A1 (en) * 2002-01-24 2003-07-31 Xianqiang Li Method for identifying multiple activated transcription factors
US20030219723A1 (en) * 2002-05-20 2003-11-27 Lu Henry H. Compositions and methods for screening and identifying anti-HCV agents
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
WO2005044978A2 (en) * 2003-07-15 2005-05-19 Washington University Methods of treating, preventing and inhibiting cancer metastasis and tumor formation
ITRM20030376A1 (it) * 2003-07-31 2005-02-01 Univ Roma Procedimento per l'isolamento e l'espansione di cellule staminali cardiache da biopsia.
US7388976B2 (en) * 2004-03-09 2008-06-17 Siemens Medical Solutions Usa, Inc. Time-based system to link periodic X-ray images
DK1754052T3 (en) * 2004-06-03 2015-09-28 Phylogica Ltd Modulators with biochemical properties
US11660317B2 (en) 2004-11-08 2023-05-30 The Johns Hopkins University Compositions comprising cardiosphere-derived cells for use in cell therapy
US8575070B2 (en) * 2006-02-20 2013-11-05 Phylogica Limited Methods of constructing and screening libraries of peptide structures
WO2008034161A1 (en) * 2006-09-19 2008-03-27 Phylogica Limited Neuroprotective peptide inhibitors of ap-1 signaling and uses therefor
EP2087098A4 (de) * 2006-11-09 2010-03-31 Univ Johns Hopkins Entdifferenzierung der kardiomyozyten erwachsener säugetiere in herzstammzellen
WO2008154700A1 (en) * 2007-06-20 2008-12-24 Phylogica Limited Compositions and uses thereof for the treatment of acute respiratory distress syndrome (ards) and clinical disorders associated with therewith
US20090040006A1 (en) * 2007-08-08 2009-02-12 International Business Machines Corporation Electrical fuse with enhanced programming current divergence
US7550323B2 (en) * 2007-08-08 2009-06-23 International Business Machines Corporation Electrical fuse with a thinned fuselink middle portion
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP3753947A1 (de) 2007-09-14 2020-12-23 Adimab, LLC Synthetische antikörperbibliotheken mit rationalem design und verwendungen davon
US7838963B2 (en) * 2007-10-26 2010-11-23 International Business Machines Corporation Electrical fuse having a fully silicided fuselink and enhanced flux divergence
US20100305002A1 (en) * 2009-04-27 2010-12-02 Roswell Park Cancer Institute Reagents and Methods for Producing Bioactive Secreted Peptides
EP2553097A1 (de) 2010-03-26 2013-02-06 Integratech Proteomics, LLC Hybridsysteme mit gesteuerter freigabe
US9249392B2 (en) 2010-04-30 2016-02-02 Cedars-Sinai Medical Center Methods and compositions for maintaining genomic stability in cultured stem cells
US9845457B2 (en) 2010-04-30 2017-12-19 Cedars-Sinai Medical Center Maintenance of genomic stability in cultured stem cells
US9354228B2 (en) 2010-07-16 2016-05-31 Adimab, Llc Antibody libraries
WO2013184527A1 (en) 2012-06-05 2013-12-12 Capricor, Inc. Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy
CA2881394A1 (en) 2012-08-13 2014-02-20 Cedars-Sinai Medical Center Exosomes and micro-ribonucleic acids for tissue regeneration
CA2962444C (en) 2014-10-03 2023-09-05 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of muscular dystrophy
WO2017083874A1 (en) 2015-11-11 2017-05-18 Serimmune Inc. Methods and compositions for assessing antibody specificities
US11253551B2 (en) 2016-01-11 2022-02-22 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction
US11351200B2 (en) 2016-06-03 2022-06-07 Cedars-Sinai Medical Center CDC-derived exosomes for treatment of ventricular tachyarrythmias
WO2018057542A1 (en) 2016-09-20 2018-03-29 Cedars-Sinai Medical Center Cardiosphere-derived cells and their extracellular vesicles to retard or reverse aging and age-related disorders
US11043823B2 (en) * 2017-04-06 2021-06-22 Tesla, Inc. System and method for facilitating conditioning and testing of rechargeable battery cells
US11759482B2 (en) 2017-04-19 2023-09-19 Cedars-Sinai Medical Center Methods and compositions for treating skeletal muscular dystrophy
US11660355B2 (en) 2017-12-20 2023-05-30 Cedars-Sinai Medical Center Engineered extracellular vesicles for enhanced tissue delivery

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135854A (en) * 1987-10-29 1992-08-04 Zymogenetics, Inc. Methods of regulating protein glycosylation
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5364783A (en) * 1990-05-14 1994-11-15 Massachusetts Institute Of Technology Retrovirus promoter-trap vectors
US5401629A (en) 1990-08-07 1995-03-28 The Salk Institute Biotechnology/Industrial Associates, Inc. Assay methods and compositions useful for measuring the transduction of an intracellular signal
US5217889A (en) * 1990-10-19 1993-06-08 Roninson Igor B Methods and applications for efficient genetic suppressor elements
WO1993008701A1 (en) * 1991-10-30 1993-05-13 The General Hospital Corporation C-myc dna binding partners, motifs, screening assays, and uses thereof
GB9123987D0 (en) 1991-11-12 1992-01-02 Primm Srl New reporter genes
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
EP0733057A4 (de) * 1993-12-16 1998-08-19 Cold Spring Harbor Lab Ursprung von replikationskomplex-genen, proteinen und verfahren
JP3468523B2 (ja) * 1994-01-21 2003-11-17 イコス コーポレイション ガゼインキナーゼ▲ｉ▼と相互作用するタンパク質に関連する方法および物質
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5691137A (en) * 1994-08-30 1997-11-25 Brandeis University Methods of screening candidate agents for biological activity using yeast cells
US5569588A (en) * 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
DK0877752T3 (da) 1996-01-23 2003-09-15 Univ Leland Stanford Junior Fremgangsmåder til screening af transdominante effektorpeptider og RNA molekyler
US5945276A (en) 1996-04-10 1999-08-31 Signal Pharmaceuticals, Inc. Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6004808A (en) * 1996-06-21 1999-12-21 Aurora Biosciences Corporation Promiscuous G-protein compositions and their use
US5998136A (en) * 1996-08-19 1999-12-07 Arcaris, Inc. Selection systems and methods for identifying genes and gene products involved in cell proliferation
US5955275A (en) * 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US5928888A (en) 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities

Also Published As

Publication number Publication date
NO994290L (no) 1999-11-03
IL131583A0 (en) 2001-01-28
US6579675B2 (en) 2003-06-17
JP2001514510A (ja) 2001-09-11
CA2283261A1 (en) 1998-09-11
CA2283261C (en) 2002-07-02
EP0973943B1 (de) 2002-09-18
AU6543898A (en) 1998-09-22
EP0973943A1 (de) 2000-01-26
ATE224457T1 (de) 2002-10-15
AU745827B2 (en) 2002-04-11
US20020018992A1 (en) 2002-02-14
US20020098503A1 (en) 2002-07-25
WO1998039483A1 (en) 1998-09-11
NO994290D0 (no) 1999-09-03
DE69808060D1 (de) 2002-10-24
US5955275A (en) 1999-09-21
DK0973943T3 (da) 2003-01-27

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