DE69832069D1 - Electrospray assembly zur introduktion von material in zellen - Google Patents
Electrospray assembly zur introduktion von material in zellenInfo
- Publication number
- DE69832069D1 DE69832069D1 DE69832069T DE69832069T DE69832069D1 DE 69832069 D1 DE69832069 D1 DE 69832069D1 DE 69832069 T DE69832069 T DE 69832069T DE 69832069 T DE69832069 T DE 69832069T DE 69832069 D1 DE69832069 D1 DE 69832069D1
- Authority
- DE
- Germany
- Prior art keywords
- biological material
- particles
- spray
- substantially dispersed
- dispersed particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002245 particle Substances 0.000 abstract 14
- 239000012620 biological material Substances 0.000 abstract 10
- 239000007921 spray Substances 0.000 abstract 7
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2/00—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic
- B01J2/006—Coating of the granules without description of the process or the device by which the granules are obtained
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2/00—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic
- B01J2/02—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops
- B01J2/04—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops in a gaseous medium
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/025—Discharge apparatus, e.g. electrostatic spray guns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/025—Discharge apparatus, e.g. electrostatic spray guns
- B05B5/03—Discharge apparatus, e.g. electrostatic spray guns characterised by the use of gas, e.g. electrostatically assisted pneumatic spraying
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/025—Discharge apparatus, e.g. electrostatic spray guns
- B05B5/053—Arrangements for supplying power, e.g. charging power
- B05B5/0533—Electrodes specially adapted therefor; Arrangements of electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/08—Plant for applying liquids or other fluent materials to objects
- B05B5/081—Plant for applying liquids or other fluent materials to objects specially adapted for treating particulate materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/08—Plant for applying liquids or other fluent materials to objects
- B05B5/082—Plant for applying liquids or other fluent materials to objects characterised by means for supporting, holding or conveying the objects
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/08—Plant for applying liquids or other fluent materials to objects
- B05B5/082—Plant for applying liquids or other fluent materials to objects characterised by means for supporting, holding or conveying the objects
- B05B5/084—Plant for applying liquids or other fluent materials to objects characterised by means for supporting, holding or conveying the objects the objects lying on, or being supported above conveying means, e.g. conveyor belts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B5/00—Electrostatic spraying apparatus; Spraying apparatus with means for charging the spray electrically; Apparatus for spraying liquids or other fluent materials by other electric means
- B05B5/08—Plant for applying liquids or other fluent materials to objects
- B05B5/087—Arrangements of electrodes, e.g. of charging, shielding, collecting electrodes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
- C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00364—Pipettes
- B01J2219/00371—Pipettes comprising electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00436—Maskless processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B13/00—Machines or plants for applying liquids or other fluent materials to surfaces of objects or other work by spraying, not covered by groups B05B1/00 - B05B11/00
- B05B13/02—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work
- B05B13/04—Means for supporting work; Arrangement or mounting of spray heads; Adaptation or arrangement of means for feeding work the spray heads being moved during spraying operation
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4944497P | 1997-06-12 | 1997-06-12 | |
US49444P | 1997-06-12 | ||
PCT/US1998/011703 WO1998056894A1 (en) | 1997-06-12 | 1998-06-05 | Electrospraying apparatus and method for introducing material into cells |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69832069D1 true DE69832069D1 (de) | 2005-12-01 |
DE69832069T2 DE69832069T2 (de) | 2006-05-24 |
Family
ID=21959851
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69832069T Expired - Lifetime DE69832069T2 (de) | 1997-06-12 | 1998-06-05 | Elektrospray-anordnung zur einführung von material in zellen |
Country Status (6)
Country | Link |
---|---|
US (6) | US6433154B1 (de) |
EP (2) | EP0988370B1 (de) |
AT (1) | ATE307878T1 (de) |
AU (1) | AU8059998A (de) |
DE (1) | DE69832069T2 (de) |
WO (1) | WO1998056894A1 (de) |
Families Citing this family (229)
Publication number | Priority date | Publication date | Assignee | Title |
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US6433154B1 (en) | 1997-06-12 | 2002-08-13 | Bristol-Myers Squibb Company | Functional receptor/kinase chimera in yeast cells |
JP4433100B2 (ja) * | 1997-06-20 | 2010-03-17 | ニューヨーク ユニヴァーシティ | チップ及びライブラリの大量製造における物質溶液の静電噴霧 |
AU1704200A (en) | 1998-09-23 | 2000-04-10 | Wisconsin Alumni Research Foundation | Charge reduction in electrospray mass spectrometry |
NL1010833C2 (nl) * | 1998-12-17 | 2000-06-20 | Univ Delft Tech | Werkwijze voor het gedoseerd aanbrengen van een vloeistof op een oppervlak. |
US20020081732A1 (en) * | 2000-10-18 | 2002-06-27 | Bowlin Gary L. | Electroprocessing in drug delivery and cell encapsulation |
US20040018226A1 (en) * | 1999-02-25 | 2004-01-29 | Wnek Gary E. | Electroprocessing of materials useful in drug delivery and cell encapsulation |
US6592623B1 (en) | 1999-08-31 | 2003-07-15 | Virginia Commonwealth University Intellectual Property Foundation | Engineered muscle |
US20040116032A1 (en) * | 1999-02-25 | 2004-06-17 | Bowlin Gary L. | Electroprocessed collagen |
US7615373B2 (en) | 1999-02-25 | 2009-11-10 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed collagen and tissue engineering |
SE9902817D0 (sv) | 1999-07-30 | 1999-07-30 | A & Science Invest Ab | A method for selective electrofusion of at least two fusion partners having cell-like membranes |
US7314969B2 (en) | 1999-11-29 | 2008-01-01 | Midwest Oilseeds, Inc. | Methods and compositions for the introduction of molecules into cells |
ATE538205T1 (de) * | 1999-11-29 | 2012-01-15 | Midwest Oilseeds Inc | Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen |
US6670607B2 (en) * | 2000-01-05 | 2003-12-30 | The Research Foundation Of State University Of New York | Conductive polymer coated nano-electrospray emitter |
CA2409093C (en) * | 2000-05-16 | 2009-07-21 | Regents Of The University Of Minnesota | High mass throughput particle generation using multiple nozzle spraying |
EP1315756A2 (de) * | 2000-09-01 | 2003-06-04 | Virginia Commonwealth University Intellectual Property Foundation | Elektroplattierte fibrin-basierte träger und gewebe |
ATE402002T1 (de) * | 2000-11-17 | 2008-08-15 | Univ Virginia Commonwealth | Elektroverarbeitetes kollagen |
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US6649907B2 (en) | 2001-03-08 | 2003-11-18 | Wisconsin Alumni Research Foundation | Charge reduction electrospray ionization ion source |
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US7078679B2 (en) * | 2002-11-27 | 2006-07-18 | Wisconsin Alumni Research Foundation | Inductive detection for mass spectrometry |
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US7312440B2 (en) * | 2003-01-14 | 2007-12-25 | Georgia Tech Research Corporation | Integrated micro fuel processor and flow delivery infrastructure |
US7208727B2 (en) * | 2003-01-14 | 2007-04-24 | Georgia Tech Research Corporation | Electrospray systems and methods |
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EP1462801A3 (de) * | 2003-03-24 | 2005-01-05 | Tepnel Lifecodes | Methoden zur messung der negativkontrolle in mehrfachanalyt-assays |
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-
1998
- 1998-05-27 US US09/085,720 patent/US6433154B1/en not_active Expired - Fee Related
- 1998-06-05 EP EP98928912A patent/EP0988370B1/de not_active Expired - Lifetime
- 1998-06-05 AU AU80599/98A patent/AU8059998A/en not_active Abandoned
- 1998-06-05 AT AT98928912T patent/ATE307878T1/de not_active IP Right Cessation
- 1998-06-05 US US09/092,794 patent/US6093557A/en not_active Expired - Lifetime
- 1998-06-05 WO PCT/US1998/011703 patent/WO1998056894A1/en active IP Right Grant
- 1998-06-05 EP EP05019449A patent/EP1616620A3/de not_active Withdrawn
- 1998-06-05 DE DE69832069T patent/DE69832069T2/de not_active Expired - Lifetime
-
2000
- 2000-05-23 US US09/577,747 patent/US6399362B1/en not_active Expired - Lifetime
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2002
- 2002-06-03 US US10/162,031 patent/US6746869B2/en not_active Expired - Lifetime
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2004
- 2004-03-25 US US10/809,660 patent/US7279322B2/en not_active Expired - Fee Related
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2007
- 2007-10-04 US US11/906,998 patent/US7972661B2/en not_active Expired - Fee Related
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US7972661B2 (en) | 2011-07-05 |
US20080141936A1 (en) | 2008-06-19 |
US6093557A (en) | 2000-07-25 |
DE69832069T2 (de) | 2006-05-24 |
US20020150669A1 (en) | 2002-10-17 |
US6399362B1 (en) | 2002-06-04 |
EP0988370A1 (de) | 2000-03-29 |
WO1998056894A1 (en) | 1998-12-17 |
ATE307878T1 (de) | 2005-11-15 |
EP1616620A3 (de) | 2006-05-10 |
US7279322B2 (en) | 2007-10-09 |
US20040177807A1 (en) | 2004-09-16 |
EP1616620A2 (de) | 2006-01-18 |
EP0988370B1 (de) | 2005-10-26 |
US6433154B1 (en) | 2002-08-13 |
AU8059998A (en) | 1998-12-30 |
US6746869B2 (en) | 2004-06-08 |
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