DE69909971D1 - Kanallose trennung von biopartikeln auf einem bioelektronischen chip durch dielektrophoresis - Google Patents

Kanallose trennung von biopartikeln auf einem bioelektronischen chip durch dielektrophoresis

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Publication number
DE69909971D1
DE69909971D1 DE69909971T DE69909971T DE69909971D1 DE 69909971 D1 DE69909971 D1 DE 69909971D1 DE 69909971 T DE69909971 T DE 69909971T DE 69909971 T DE69909971 T DE 69909971T DE 69909971 D1 DE69909971 D1 DE 69909971D1
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DE
Germany
Prior art keywords
chip
cartridge
cell
dielectrophoresis
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69909971T
Other languages
English (en)
Inventor
Jing C O Aviva Bioscienc Cheng
L Sheldon
Lei Wu
P O'connell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
Original Assignee
Nanogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanogen Inc filed Critical Nanogen Inc
Application granted granted Critical
Publication of DE69909971D1 publication Critical patent/DE69909971D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
    • H01L29/00—Semiconductor devices adapted for rectifying, amplifying, oscillating or switching, or capacitors or resistors with at least one potential-jump barrier or surface barrier, e.g. PN junction depletion layer or carrier concentration layer; Details of semiconductor bodies or of electrodes thereof  ; Multistep manufacturing processes therefor
    • H01L29/66—Types of semiconductor device ; Multistep manufacturing processes therefor
    • H01L29/66007—Multistep manufacturing processes
    • H01L29/66075—Multistep manufacturing processes of devices having semiconductor bodies comprising group 14 or group 13/15 materials
    • H01L29/66227—Multistep manufacturing processes of devices having semiconductor bodies comprising group 14 or group 13/15 materials the devices being controllable only by the electric current supplied or the electric potential applied, to an electrode which does not carry the current to be rectified, amplified or switched, e.g. three-terminal devices
    • H01L29/66409—Unipolar field-effect transistors
    • H01L29/66477—Unipolar field-effect transistors with an insulated gate, i.e. MISFET
    • H01L29/6656—Unipolar field-effect transistors with an insulated gate, i.e. MISFET using multiple spacer layers, e.g. multiple sidewall spacers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00853—Employing electrode arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00905—Separation
    • B01J2219/00912—Separation by electrophoresis
    • B01J2219/00914—Separation by electrophoresis by dielectrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0493—Specific techniques used
    • B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
DE69909971T 1998-01-30 1999-01-26 Kanallose trennung von biopartikeln auf einem bioelektronischen chip durch dielektrophoresis Expired - Lifetime DE69909971D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/016,596 US6071394A (en) 1996-09-06 1998-01-30 Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
PCT/US1999/001651 WO1999038612A1 (en) 1998-01-30 1999-01-26 Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

Publications (1)

Publication Number Publication Date
DE69909971D1 true DE69909971D1 (de) 2003-09-04

Family

ID=21777962

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69909971T Expired - Lifetime DE69909971D1 (de) 1998-01-30 1999-01-26 Kanallose trennung von biopartikeln auf einem bioelektronischen chip durch dielektrophoresis

Country Status (12)

Country Link
US (3) US6071394A (de)
EP (1) EP1053055B1 (de)
JP (1) JP2002502047A (de)
KR (1) KR100512518B1 (de)
CN (1) CN1170942C (de)
AT (1) ATE246040T1 (de)
AU (1) AU743074B2 (de)
BR (1) BR9908349A (de)
CA (1) CA2319705C (de)
DE (1) DE69909971D1 (de)
NZ (1) NZ505858A (de)
WO (1) WO1999038612A1 (de)

Families Citing this family (216)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197508B1 (en) 1990-09-12 2001-03-06 Affymetrix, Inc. Electrochemical denaturation and annealing of nucleic acid
US6129828A (en) 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US7314708B1 (en) * 1998-08-04 2008-01-01 Nanogen, Inc. Method and apparatus for electronic synthesis of molecular structures
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US6071394A (en) * 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US6355436B1 (en) 1996-05-17 2002-03-12 L'ecole Centrale De Lyon Method for analyzing biological substances in a conductive liquid medium
WO1999025816A1 (en) * 1997-11-14 1999-05-27 California Institute Of Technology Cell lysis device
US20020144905A1 (en) * 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
JP3486171B2 (ja) * 1997-12-17 2004-01-13 エコル・ポリテクニック・フェデラル・ドゥ・ロザンヌ（エ・ペー・エフ・エル） ミクロ構造キャリア上における細胞単体および再構成膜系のポジショニングおよび電気生理学的特性決定
US20050053962A1 (en) * 1998-01-27 2005-03-10 Gary Blackburn Amplification of nucleic acids with electronic detection
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6387632B2 (en) * 1998-06-11 2002-05-14 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor
US6093370A (en) * 1998-06-11 2000-07-25 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor
GB9812783D0 (en) * 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
US20020012916A1 (en) * 1998-10-14 2002-01-31 Gundling Gerard J A method of reducing contamination in an essay vessel
BR9916840A (pt) * 1998-12-23 2001-10-09 Nanogen Inc Sistema de detecção biológica portátil integrado
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
CN1181337C (zh) 2000-08-08 2004-12-22 清华大学 微流体系统中实体分子的操纵方法及相关试剂盒
US6858439B1 (en) * 1999-03-15 2005-02-22 Aviva Biosciences Compositions and methods for separation of moieties on chips
US6821770B1 (en) 1999-05-03 2004-11-23 Gen-Probe Incorporated Polynucleotide matrix-based method of identifying microorganisms
US6326486B1 (en) 1999-05-03 2001-12-04 Gen-Probe Incorporated Polynucleotide probes for detection and quantitation of bacteria in the family enterobacteriaceae
US6811668B1 (en) 1999-06-22 2004-11-02 Caliper Life Sciences, Inc. Apparatus for the operation of a microfluidic device
EP1360992A3 (de) * 1999-06-22 2004-05-19 Caliper Life Sciences, Inc. Apparat zur Bedienung eines mikrofluidischen Gerätes
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US20040185462A1 (en) * 1999-08-06 2004-09-23 Tum Gene, Inc. Method of and detecting apparatus and detecting chip for single base substitution SNP and point mutation of genes
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6495104B1 (en) * 1999-08-19 2002-12-17 Caliper Technologies Corp. Indicator components for microfluidic systems
EP1080785A1 (de) * 1999-09-04 2001-03-07 F. Hoffmann-La Roche Ag Thermozyklisches System für Fluide in Patronen
US20030232388A1 (en) * 1999-09-27 2003-12-18 Kreimer David I. Beads having identifiable Raman markers
US20020132371A1 (en) * 1999-09-27 2002-09-19 Kreimer David I. Amplification of analyte detection by substrates having particle structures with receptors
US20040023293A1 (en) * 1999-09-27 2004-02-05 Kreimer David I. Biochips for characterizing biological processes
ATE408141T1 (de) 1999-10-20 2008-09-15 Shigeori Takenaka Gen-detektionschip, sensor und nachweisverfahren
US6824664B1 (en) 1999-11-04 2004-11-30 Princeton University Electrode-less dielectrophorises for polarizable particles
WO2001040786A1 (en) * 1999-12-01 2001-06-07 The Regents Of The University Of California Electric-field-assisted fluidic assembly of inorganic and organic materials, molecules and like small things including living cells
WO2001045843A2 (en) * 1999-12-22 2001-06-28 Gene Logic, Inc. Flow-thru chip cartridge, chip holder, system and method thereof
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
US6537433B1 (en) * 2000-03-10 2003-03-25 Applera Corporation Methods and apparatus for the location and concentration of polar analytes using an alternating electric field
PT1679196E (pt) * 2000-06-15 2008-07-21 Seiko Epson Corp Processo de carregamento de líquidos, recipiente de líquidos e processo para fabricar o mesmo
US6602400B1 (en) 2000-06-15 2003-08-05 Motorola, Inc. Method for enhanced bio-conjugation events
US6632400B1 (en) * 2000-06-22 2003-10-14 Agilent Technologies, Inc. Integrated microfluidic and electronic components
AU785424B2 (en) * 2000-07-26 2007-05-17 Regents Of The University Of California, The Manipulation of live cells and inorganic objects with optical micro beam arrays
US7270730B2 (en) * 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
AU2000274922A1 (en) * 2000-08-08 2002-02-18 Aviva Biosciences Corporation Methods for manipulating moieties in microfluidic systems
CN100495030C (zh) 2000-09-30 2009-06-03 清华大学 多力操纵装置及其应用
US20020076825A1 (en) * 2000-10-10 2002-06-20 Jing Cheng Integrated biochip system for sample preparation and analysis
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
US6784420B2 (en) * 2000-11-13 2004-08-31 Genoptix, Inc. Method of separating particles using an optical gradient
US20020160470A1 (en) * 2000-11-13 2002-10-31 Genoptix Methods and apparatus for generating and utilizing linear moving optical gradients
US6936811B2 (en) * 2000-11-13 2005-08-30 Genoptix, Inc. Method for separating micro-particles
US6833542B2 (en) * 2000-11-13 2004-12-21 Genoptix, Inc. Method for sorting particles
US20020123112A1 (en) * 2000-11-13 2002-09-05 Genoptix Methods for increasing detection sensitivity in optical dielectric sorting systems
US6744038B2 (en) 2000-11-13 2004-06-01 Genoptix, Inc. Methods of separating particles using an optical gradient
US6778724B2 (en) * 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
US6881317B2 (en) * 2000-12-18 2005-04-19 The Trustees Of Princeton University Fractionation of macro-molecules using asymmetric pulsed field electrophoresis
ES2269093T3 (es) 2000-12-28 2007-04-01 F. Hoffmann-La Roche Ag Metodo, sistema y cartucho para el procedimiento de una muestra de acido nucleico mediante la oscilacion del cartucho.
US6707548B2 (en) 2001-02-08 2004-03-16 Array Bioscience Corporation Systems and methods for filter based spectrographic analysis
US6626417B2 (en) 2001-02-23 2003-09-30 Becton, Dickinson And Company Microfluidic valve and microactuator for a microvalve
US7297518B2 (en) * 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7014742B2 (en) * 2001-03-15 2006-03-21 The Regents Of The University Of California Positioning of organic and inorganic objects by electrophoretic forces, including for microlens alignment
WO2002074899A1 (en) * 2001-03-15 2002-09-26 Array Bioscience Corporation Enhancing surfaces for analyte detection
CN100494360C (zh) * 2001-03-22 2009-06-03 博奥生物有限公司 细胞分离方法及其应用
CA2441366A1 (en) 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20030153024A1 (en) * 2001-04-19 2003-08-14 Sullivan Brian M Charged bio-molecule/binding agent conjugate for biological capture
US20030194755A1 (en) * 2001-04-27 2003-10-16 Genoptix, Inc. Early detection of apoptotic events and apoptosis using optophoretic analysis
US20040009540A1 (en) * 2001-04-27 2004-01-15 Genoptix, Inc Detection and evaluation of cancer cells using optophoretic analysis
ITTO20010411A1 (it) * 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
JP3920221B2 (ja) * 2001-05-02 2007-05-30 アプレラ コーポレイション 電場を使用する分析物の濃縮および精製
DE10122659A1 (de) * 2001-05-10 2002-12-05 Infineon Technologies Ag Biochip-Anordnung
US7419574B2 (en) * 2001-06-20 2008-09-02 Cummings Eric B Dielectrophoresis device and method having non-uniform arrays for manipulating particles
US7014747B2 (en) * 2001-06-20 2006-03-21 Sandia Corporation Dielectrophoretic systems without embedded electrodes
US20030158133A1 (en) * 2001-08-01 2003-08-21 Movsesian Matthew A. Isoform-selective inhibitors and activators of PDE3 cyclic nucleotide phosphodiesterases
US6890409B2 (en) * 2001-08-24 2005-05-10 Applera Corporation Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices
US8980568B2 (en) 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
US8986944B2 (en) * 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
WO2003035228A1 (en) * 2001-10-19 2003-05-01 Trustees Of Princeton University Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules
FR2831084B1 (fr) * 2001-10-22 2004-08-27 Centre Nat Rech Scient Procede et systeme pour manipuler par dielectrophorese des particules dielectriques, en particulier des cellules biologiques
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US6866762B2 (en) * 2001-12-20 2005-03-15 Board Of Regents, University Of Texas System Dielectric gate and methods for fluid injection and control
EP1472529A1 (de) * 2002-01-04 2004-11-03 Board Of Regents The University Of Texas System Wandlose kanäle zum fluidischen routen und eingrenzen
DE10204652B4 (de) 2002-02-05 2004-07-22 Infineon Technologies Ag Schaltkreis-Anordnung, elektrochemischer Sensor, Sensor-Anordnung und Verfahren zum Verarbeiten eines über eine Sensor-Elektrode bereitgestellten Stromsignals
DE10321490B3 (de) * 2002-02-05 2004-10-14 Infineon Technologies Ag Schaltkreis-Anordnung, elektrochemischer Sensor, Sensor-Anordnung und Verfahren zum Verarbeiten eines über eine Sensor-Elektrode bereitgestellten Stromsignals
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
CA2474530A1 (en) * 2002-02-07 2003-08-14 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Diagnostic microarray and method of use thereof
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20050100938A1 (en) * 2002-03-14 2005-05-12 Infineon Technologies Ag Vertical impedance sensor arrangement and method for producing a vertical impedance sensor arrangement
US20030211461A1 (en) * 2002-05-01 2003-11-13 Genoptix, Inc Optophoretic detection of durgs exhibiting inhibitory effect on Bcr-Abl positive tumor cells
US20040033539A1 (en) * 2002-05-01 2004-02-19 Genoptix, Inc Method of using optical interrogation to determine a biological property of a cell or population of cells
US20040011650A1 (en) * 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
GB0218009D0 (en) * 2002-08-02 2002-09-11 Imp College Innovations Ltd DNA extraction microchip, system and method
US20040053209A1 (en) * 2002-09-12 2004-03-18 Genoptix, Inc Detection and evaluation of topoisomerase inhibitors using optophoretic analysis
JP2005538717A (ja) * 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド 核酸のプレパラティブスケールの精製のための装置および方法
WO2004027379A2 (en) * 2002-09-20 2004-04-01 Novus Molecular, Inc. Methods and devices for active bioassay
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
EP1569510B1 (de) 2002-09-27 2011-11-02 The General Hospital Corporation Mikrofluidische vorrichtung zur zelltrennung und verwendungen davon
US20040067167A1 (en) * 2002-10-08 2004-04-08 Genoptix, Inc. Methods and apparatus for optophoretic diagnosis of cells and particles
CN1429917A (zh) * 2002-10-11 2003-07-16 薛永新 艾滋病毒原位核酸芯片及其制备方法
US7932098B2 (en) * 2002-10-31 2011-04-26 Hewlett-Packard Development Company, L.P. Microfluidic system utilizing thin-film layers to route fluid
US20040086872A1 (en) * 2002-10-31 2004-05-06 Childers Winthrop D. Microfluidic system for analysis of nucleic acids
US20040121474A1 (en) * 2002-12-19 2004-06-24 Genoptix, Inc Detection and evaluation of chemically-mediated and ligand-mediated t-cell activation using optophoretic analysis
US20040121307A1 (en) * 2002-12-19 2004-06-24 Genoptix, Inc Early detection of cellular differentiation using optophoresis
US7413891B2 (en) * 2003-04-30 2008-08-19 Biovitesse, Inc. Apparatus and method for detecting live cells with an integrated filter and growth detection device
US20050009066A1 (en) * 2003-05-15 2005-01-13 Connolly Dennis M. Methods for localizing target molecules in a flowing fluid sample
JP4533382B2 (ja) * 2003-08-28 2010-09-01 セルラ・インコーポレイテッド マイクロ流体分析およびソーティング用の一体化された構造物
US7347923B2 (en) * 2003-10-03 2008-03-25 Sandia Corporation Dielectrophoresis device and method having insulating ridges for manipulating particles
US20050287668A1 (en) * 2003-11-04 2005-12-29 Cell Therapeutics, Inc. (Cti) RNA interference compositions and screening methods for the identification of novel genes and biological pathways
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
JP2007526823A (ja) 2004-02-02 2007-09-20 ザ ユニバーシティ オブ ブリティッシュ コロンビア 粒子を運動および濃縮させるためのスコダ泳動ならびに方法および装置
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
EP1730276B8 (de) * 2004-02-26 2010-07-21 DELTA Dansk Elektronik, Lys og Akustik Verfahren, chip, vorrichtung und system zur extraktion biologischer materialien
ATE474223T1 (de) 2004-02-26 2010-07-15 Thomsen Bioscience As Verfahren, chip und system zum sammeln biologischer partikel
CA2557485A1 (en) 2004-02-26 2005-09-09 Thomsen Bioscience A/S Method, chip, device and integrated system for detection biological particles
US7187286B2 (en) 2004-03-19 2007-03-06 Applera Corporation Methods and systems for using RFID in biological field
EP3144066A1 (de) 2004-05-28 2017-03-22 Board of Regents, The University of Texas System Programmierbare fluidverarbeiter
FR2871070B1 (fr) * 2004-06-02 2007-02-16 Commissariat Energie Atomique Microdispositif et procede de separation d'emulsion
WO2006017274A2 (en) * 2004-07-13 2006-02-16 U.S. Genomics, Inc. Systems and methods for sample modification using fluidic chambers
ITPD20040301A1 (it) * 2004-11-26 2005-02-26 Dimensional Srl P Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale
KR100594408B1 (ko) 2004-12-17 2006-06-30 한국과학기술연구원 초음파장 및 진행파 유전영동을 이용한 세포 분리 장치
KR100738071B1 (ko) * 2005-01-21 2007-07-12 삼성전자주식회사 농도구배발생부가 구비된 유전영동 장치, 그를 이용한물질의 분리방법 및 물질 분리의 최적 조건을 탐색하는 방법
PL1859330T3 (pl) 2005-01-28 2013-01-31 Univ Duke Urządzenia i sposoby manipulacji kropelkami na płytkach obwodów drukowanych
CA2496294A1 (en) * 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
JP2006242925A (ja) 2005-03-07 2006-09-14 Seiko Epson Corp 電極基板、該基板を備える検出装置および該装置を含むキット、並びに該キットを用いた検出方法
JP2006242926A (ja) * 2005-03-07 2006-09-14 Seiko Epson Corp 電極基板、該基板を備える検出装置および該装置を含むキット、並びに該キットを用いた検出方法
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1764418B1 (de) * 2005-09-14 2012-08-22 STMicroelectronics Srl Verfahren und Vorrichtung zur Behandlung biologischer Proben mittels Dielektrophorese
US20070090026A1 (en) * 2005-10-06 2007-04-26 Jongyoon Han Continuous biomolecule separation in a nanofilter
CN101365781A (zh) * 2005-11-21 2009-02-11 阿普尔拉公司 用于核酸处理的便携式制备、分析和检测设备
KR101157175B1 (ko) * 2005-12-14 2012-07-03 삼성전자주식회사 세포 또는 바이러스의 농축 및 용해용 미세유동장치 및방법
JP4748451B2 (ja) * 2006-02-08 2011-08-17 凸版印刷株式会社 ハイブリダイゼーションの検出方法
US20080003655A1 (en) 2006-04-14 2008-01-03 University Of South Florida Microbial Cell and Particle Control
JP4813244B2 (ja) * 2006-04-25 2011-11-09 シャープ株式会社 サンプル分離吸着器具
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
CA2657621A1 (en) * 2006-07-14 2008-01-17 Aviva Biosciences Corporation Methods and compositions for detecting rare cells from a biological sample
WO2008085991A2 (en) * 2007-01-08 2008-07-17 U.S. Genomics, Inc. Reaction chamber
EP2130913A4 (de) * 2007-03-05 2010-09-01 On Chip Cellomics Consortium Chip zur zellkomponenten-probennahme, zellkomponenten-analysesystem und zellkomponenten-analyseverfahren unter verwendung davon
US20100294665A1 (en) * 2007-07-12 2010-11-25 Richard Allen Method and system for transferring and/or concentrating a sample
BRPI0813718A2 (pt) * 2007-07-13 2014-12-30 Univ Leland Stanford Junior Método e aparelho que utilizam um campo elétrico para ensaios biológicos aperfeiçoados
GB0714866D0 (en) * 2007-07-31 2007-09-12 Univ Leeds Biosensor
WO2009021233A2 (en) * 2007-08-09 2009-02-12 Advanced Liquid Logic, Inc. Pcb droplet actuator fabrication
US8308926B2 (en) 2007-08-20 2012-11-13 Purdue Research Foundation Microfluidic pumping based on dielectrophoresis
GB0812679D0 (en) 2008-07-10 2008-08-20 Sec Dep For Innovation Universities Sample carrier for effecting chemical assays
EP2220494B1 (de) 2007-11-26 2011-11-09 The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government Elektrochemischer nachweis mit metal markiertem nachweismittel
EP2238434A4 (de) * 2008-02-01 2011-06-22 Univ British Columbia Verfahren und vorrichtung zur partikeleinführung und -rückgewinnung
CN101231262B (zh) * 2008-02-22 2011-09-14 东南大学 用于单细胞组学的方法
GB0804491D0 (en) * 2008-03-11 2008-04-16 Iti Scotland Ltd Detecting analytes
WO2009146143A2 (en) * 2008-04-03 2009-12-03 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
CN103900894A (zh) * 2008-06-06 2014-07-02 华盛顿大学 用于从溶液浓缩颗粒的方法和系统
GB0812681D0 (en) * 2008-07-10 2008-08-20 Sec Dep For Innovation Universities Apparatus and methods for effecting chemical assays
US8361716B2 (en) * 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
US8940092B1 (en) 2008-10-27 2015-01-27 University Of Washington Through Its Center For Commercialization Hybrid fibers, devices using hybrid fibers, and methods for making hybrid fibers
US10895575B2 (en) * 2008-11-04 2021-01-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
KR101023040B1 (ko) 2008-11-13 2011-03-24 한국항공대학교산학협력단 고속 입자분리 장치 및 그 방법
US9134221B2 (en) 2009-03-10 2015-09-15 The Regents Of The University Of California Fluidic flow cytometry devices and particle sensing based on signal-encoding
WO2010121381A1 (en) 2009-04-21 2010-10-28 The University Of British Columbia System and methods for detection of particles
US9063136B2 (en) * 2009-08-02 2015-06-23 Qvella Corporation Cell concentration, capture and lysis devices and methods of use thereof
WO2012054904A2 (en) * 2010-10-21 2012-04-26 The Regents Of The University Of California Microfluidics with wirelessly powered electronic circuits
WO2012065075A2 (en) * 2010-11-12 2012-05-18 The Regents Of The University Of California Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep)
US20120219987A1 (en) * 2010-12-13 2012-08-30 Tofy Mussivand Device for electroporation and lysis
CA2742327A1 (en) 2011-05-20 2012-11-20 The University Of British Columiba Systems and methods for enhanced scoda
ES2759824T3 (es) 2011-07-25 2020-05-12 Qvella Corp Métodos y dispositivos para preparación de muestra eléctrica
US11198126B2 (en) 2011-10-31 2021-12-14 Fluid-Screen, Inc. Apparatus for pathogen detection
US9120105B2 (en) 2011-10-31 2015-09-01 Monika Weber Electronic device for pathogen detection
FR2986802A1 (fr) * 2012-02-13 2013-08-16 Centre Nat Rech Scient Dispositif et procede de manipulation et traitement de cellules par champ electrique.
CA2870160C (en) 2012-04-16 2021-08-24 Biological Dynamics, Inc. Nucleic acid sample preparation
US8932815B2 (en) 2012-04-16 2015-01-13 Biological Dynamics, Inc. Nucleic acid sample preparation
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
EP2844773B1 (de) 2012-05-04 2017-08-16 Boreal Genomics Corp. Biomarkeranalyse mittels scodaphorese
GB201217390D0 (en) 2012-09-28 2012-11-14 Agplus Diagnostics Ltd Test device and sample carrier
CN110579435B (zh) 2012-10-15 2023-09-26 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法
US8975060B2 (en) * 2012-11-30 2015-03-10 Qvella Corporation Method for pretreatment of microbial samples
US10662420B2 (en) 2012-11-30 2020-05-26 Qvella Corporation Apparatus and method for extracting microbial cells
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
CN103627788A (zh) * 2013-08-21 2014-03-12 中山大学达安基因股份有限公司 一种电化学基因传感器生物芯片的制备方法
CN106459966A (zh) 2014-04-08 2017-02-22 生物动力学公司 用于生物材料的分离的改进的装置
CN103894248B (zh) * 2014-04-09 2015-09-16 国家纳米科学中心 一种单细胞分析用微流控芯片和系统及单细胞分析方法
DK3594360T3 (da) 2014-04-24 2021-08-30 Lucira Health Inc Kolorimetrisk detektering af nukleinsyre amplifikation
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
CN104897888A (zh) * 2015-05-27 2015-09-09 合肥卓元科技服务有限公司 一种分隔式酶反应传感器系统
CN106310944B (zh) * 2015-07-01 2022-10-11 王冰 用于分离介质内颗粒和小液滴的介电电泳电极及电极阵列
CN107615041B (zh) * 2015-10-07 2020-12-11 Afi技术公司 检查装置、检查系统以及检查方法
JP6796932B2 (ja) * 2016-02-16 2020-12-09 株式会社Ａｆｉテクノロジー 分離装置
CA3015378C (en) 2016-03-14 2023-09-26 Diassess Inc. Selectively vented biological assay devices and associated methods
CN115487880A (zh) 2016-03-24 2022-12-20 生物动力学公司 一次性射流卡盘及组件
SG11201808959QA (en) 2016-04-15 2018-11-29 Fluid Screen Inc Analyte detection methods and apparatus using dielectrophoresis and electroosmosis
US10696939B2 (en) * 2016-04-22 2020-06-30 Hewlett-Packard Development Company, L.P. Cell lysis
JP6671665B2 (ja) * 2016-10-27 2020-03-25 シャープ株式会社 蛍光検査システム、誘電泳動デバイス及び分子検査方法
US11080848B2 (en) 2017-04-06 2021-08-03 Lucira Health, Inc. Image-based disease diagnostics using a mobile device
IL270445B1 (en) 2017-05-08 2024-02-01 Biological dynamics inc Methods and systems for processing information on tested material
US10549275B2 (en) 2017-09-14 2020-02-04 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
US11731132B2 (en) 2017-12-19 2023-08-22 Biological Dynamics, Inc. Methods and devices for detection of multiple analytes from a biological sample
JP7317026B2 (ja) 2018-01-30 2023-07-28 ライフ テクノロジーズ コーポレーション スマート分子分析システムのワークフローにおける使用のための機器、デバイス、および消耗品
CN117065932A (zh) 2018-04-02 2023-11-17 生物动力学公司 介电材料
USD907232S1 (en) 2018-12-21 2021-01-05 Lucira Health, Inc. Medical testing device
JP2022523964A (ja) * 2019-03-05 2022-04-27 ルシラ ヘルス インコーポレイテッド 気泡のない、流体チャンバの液体充填方法
CN111936862A (zh) * 2019-03-11 2020-11-13 京东方科技集团股份有限公司 微流道及其制备方法和操作方法
CA3161340A1 (en) 2019-11-13 2021-05-20 Fluid-Screen, Inc. An apparatus and methods to rapidly detect, separate, purify, and quantify various viruses from cells, cultured medium and other fluids
EP4058778A4 (de) 2019-11-13 2023-12-27 Fluid-Screen, Inc. Verfahren und vorrichtung zum nachweis von bakterien in einer probe mittels dielektrophorese
US11542541B2 (en) 2019-11-26 2023-01-03 Bio-Rad Laboratories, Inc. Method and system for sampling material from cells
CN111250183B (zh) * 2020-02-17 2021-04-09 北京中科生仪科技有限公司 一种微流控系统用注液泵驱动装置
USD953561S1 (en) 2020-05-05 2022-05-31 Lucira Health, Inc. Diagnostic device with LED display
CN111644212B (zh) * 2020-05-22 2022-05-24 华东理工大学 一种微流控芯片和纳米颗粒分离装置
USD962470S1 (en) 2020-06-03 2022-08-30 Lucira Health, Inc. Assay device with LCD display
WO2022020206A1 (en) * 2020-07-24 2022-01-27 Palogen, Inc. Nanochannel systems and methods for detecting pathogens using same
CN112986556B (zh) * 2021-05-12 2022-02-18 佛山微奥云生物技术有限公司 一种用于快速检测的芯片及其加工方法

Family Cites Families (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3346479A (en) * 1964-04-09 1967-10-10 Scientific Industries Preparative separation by a combination of gel separation and electrophoresis
US3375187A (en) * 1965-05-13 1968-03-26 Buchler Instr Inc Apparatus for temperature controlled preparative and analytical electrophoresis
US3533933A (en) * 1967-03-31 1970-10-13 Hannig Kurt Process and device for the isolation of fractions of a substance mixture electrophoretically separated in a carrier gel
US3539493A (en) * 1967-08-31 1970-11-10 Canal Ind Corp Apparatus for preparative electrophoresis on gel support media
US3627137A (en) 1968-10-10 1971-12-14 Milan Bier Dialyzing membrane envelope
DE1807481C3 (de) * 1968-11-07 1975-10-09 Messerschmitt-Boelkow-Blohm Gmbh, 8000 Muenchen Verfahren zum partiellen Galvanisieren
US3616454A (en) * 1969-03-13 1971-10-26 Univ New York Method of and apparatus for electrophoretic separation in a gel column
US3640813A (en) * 1969-06-09 1972-02-08 Samuel T Nerenberg Adapter for a macromolecule separation device
DE2051715B2 (de) 1970-10-21 1976-01-15 Trennkammer fuer die kontinuierliche ablenkungselektrophorese
JPS4821679U (de) 1971-07-16 1973-03-12
NL7115364A (de) * 1971-11-08 1973-05-10
DE2221242C3 (de) * 1972-04-29 1974-09-26 Stephan 8520 Erlangen Nees Gerät für die präparat!ve Elektrophorese und Verfahren zu deren Durchführung mit diesem Gerät
SU434985A1 (ru) 1972-06-07 1974-07-05 Устройство для препаративного разделения высокомолекулярных биологических веществ
GB1359944A (en) 1972-07-18 1974-07-17 Miles Lab Apparatus for gel electrophoresis
US3795600A (en) * 1973-03-23 1974-03-05 Instrumentation Specialties Co Electrophoresis and method apparatus
US3980546A (en) * 1975-06-27 1976-09-14 Caccavo Francis A Micropreparative electrophoresis apparatus
NL182822C (nl) * 1976-05-18 1988-05-16 Roskam Willem Gerrit Preparatief elektroforese-apparaat.
SU616568A1 (ru) 1977-01-24 1978-07-25 Специальное Конструкторское Бюро Биофизической Аппаратуры Устройство дл электрофоретического разделени веществ
US4326934A (en) 1979-12-31 1982-04-27 Pohl Herbert A Continuous dielectrophoretic cell classification method
US4390403A (en) * 1981-07-24 1983-06-28 Batchelder J Samuel Method and apparatus for dielectrophoretic manipulation of chemical species
JPS58147639A (ja) 1982-02-26 1983-09-02 Hideyuki Nishizawa 連続電気泳動分離法および装置
DE3374082D1 (en) * 1982-07-23 1987-11-19 Matthias Hediger Apparatus for preparative gel electrophoresis, in particular for separating mixtures on a gradient gel polyacryl amide column
US4441972A (en) * 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4661451A (en) * 1984-02-06 1987-04-28 Ortho Diagnostic Systems, Inc. Methods for immobilizing and translocating biological cells
EP0155833A3 (de) 1984-03-15 1988-07-27 Fuji Photo Film Co., Ltd. Bestandteil für Elektrophorese
FR2574311B1 (fr) 1984-12-11 1989-09-15 Inst Nat Sante Rech Med Perfectionnements aux procedes et appareils d'electrophorese preparative
US4617102A (en) 1985-03-21 1986-10-14 Lifecodes Corp. Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA
US4740283A (en) * 1986-02-27 1988-04-26 University Patents, Inc. Pulsed-field gradient gel electrophoretic apparatus
US4699706A (en) * 1986-05-30 1987-10-13 Bio-Rad Laboratories, Inc. Method and apparatus for extraction of species from electrophoresis media
US5593580A (en) 1986-11-26 1997-01-14 Kopf; Henry B. Filtration cassette article, and filter comprising same
DE3876273T2 (de) * 1987-04-11 1993-05-27 Ciba Geigy Ag Isoelektrisches fokussierverfahren sowie einrichtung zur durchfuehrung dieses verfahrens.
US4787963A (en) * 1987-05-04 1988-11-29 Syntro Corporation Method and means for annealing complementary nucleic acid molecules at an accelerated rate
US4936963A (en) 1987-05-27 1990-06-26 Abbott Laboratories Polycationic buffers and method for gel electrophoresis of nucleic acids
US5202010A (en) 1987-11-25 1993-04-13 Princeton Biochemicals, Inc. Automated capillary electrophoresis apparatus
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5382511A (en) * 1988-08-02 1995-01-17 Gene Tec Corporation Method for studying nucleic acids within immobilized specimens
US4877510A (en) * 1988-10-25 1989-10-31 Bio-Rad Laboratories, Inc. Apparatus for preparative gel electrophoresis
US5062942A (en) * 1989-04-12 1991-11-05 Hitachi, Ltd. Fluorescence detection type electrophoresis apparatus
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5344535A (en) * 1989-11-27 1994-09-06 British Technology Group Limited Dielectrophoretic characterization of micro-organisms and other particles
US5795457A (en) * 1990-01-30 1998-08-18 British Technology Group Ltd. Manipulation of solid, semi-solid or liquid materials
US5126022A (en) * 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5085756A (en) * 1990-08-21 1992-02-04 Hewlett-Packard Company Column separation system for electrophoresis with sample pretreatment
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5151189A (en) * 1990-09-17 1992-09-29 Gelman Sciences, Inc. Cationic charge modified microporous membrane
US5269931A (en) * 1990-09-17 1993-12-14 Gelman Sciences Inc. Cationic charge modified microporous membranes
US5340449A (en) * 1990-12-07 1994-08-23 Shukla Ashok K Apparatus for electroelution
JP3104995B2 (ja) * 1991-01-31 2000-10-30 ワーナー−ランバート・コンパニー 抗炎症剤として有用な４，６−ジ−第三ブチル−５−ヒドロキシ−１，３−ピリミジンの置換されたヘテロアリール類似体
US5139637A (en) * 1991-03-13 1992-08-18 Macconnell William P Plasmid purification system and method
US5217593A (en) * 1991-03-13 1993-06-08 Macconnell William P Nucleic acid purification system and method
US5078853A (en) 1991-03-22 1992-01-07 Assay Technology, Inc. Segmented electrophoretic separation system useful for lipid profiling
US5209831A (en) * 1991-06-14 1993-05-11 Macconnell William P Bufferless electrophoresis system and method
US5161165A (en) * 1991-09-26 1992-11-03 Hewlett-Packard Company Multimode stabilized external cavity laser
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6048690A (en) 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
EP0544969B1 (de) 1991-12-06 1997-03-05 Ciba-Geigy Ag Elektrophoretische Trennvorrichtung und elektrophoretisches Trennverfahren
JPH05236997A (ja) * 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
GB9208357D0 (en) * 1992-04-16 1992-06-03 British Tech Group Apparatus for separating a mixture
US5340499A (en) 1992-08-11 1994-08-23 Neste Oy Electrically conductive compositions and methods for their preparation
US5376249A (en) * 1992-11-25 1994-12-27 Perseptive Biosystems, Inc. Analysis utilizing isoelectric focusing
GB9301122D0 (en) * 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
GB9306729D0 (en) * 1993-03-31 1993-05-26 British Tech Group Improvements in separators
US5427664A (en) 1993-07-22 1995-06-27 Stoev; Stoyan V. Free solution electrophoresis-membrane filters trapping assay apparatus and method
US6309601B1 (en) 1993-11-01 2001-10-30 Nanogen, Inc. Scanning optical detection system
US6726880B1 (en) 1993-11-01 2004-04-27 Nanogen, Inc. Electronic device for performing active biological operations and method of using same
US6099803A (en) 1994-07-07 2000-08-08 Nanogen, Inc. Advanced active electronic devices for molecular biological analysis and diagnostics
US6254827B1 (en) 1993-11-01 2001-07-03 Nanogen, Inc. Methods for fabricating multi-component devices for molecular biological analysis and diagnostics
DE4400955C2 (de) * 1993-12-23 1999-04-01 Fraunhofer Ges Forschung Adhäsionssteuerbare Oberflächenstruktur
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US5728267A (en) 1994-11-15 1998-03-17 Flaherty; James E. Concentrator for separating small samples in a centrifuge
CA2230375A1 (en) * 1995-08-25 1997-03-06 Scientific Generics Limited Release of intracellular material
US5858192A (en) * 1996-10-18 1999-01-12 Board Of Regents, The University Of Texas System Method and apparatus for manipulation using spiral electrodes
AU6751800A (en) 1999-08-13 2001-03-13 Aventis Research And Technologies Gmbh Microelectronic molecular descriptor array devices, methods, procedures, and formats for combinatorial selection of intermolecular ligand binding structures and for drug screening
US6960298B2 (en) 2001-12-10 2005-11-01 Nanogen, Inc. Mesoporous permeation layers for use on active electronic matrix devices
US20040011650A1 (en) 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis

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KR20010040474A (ko) 2001-05-15
BR9908349A (pt) 2000-12-05
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AU743074B2 (en) 2002-01-17
US6989086B2 (en) 2006-01-24
EP1053055A1 (de) 2000-11-22
WO1999038612A1 (en) 1999-08-05
AU2344599A (en) 1999-08-16
US20010045359A1 (en) 2001-11-29
CA2319705A1 (en) 1999-08-05
US6280590B1 (en) 2001-08-28
CA2319705C (en) 2008-07-15
ATE246040T1 (de) 2003-08-15
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CN1291913A (zh) 2001-04-18
US6071394A (en) 2000-06-06

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