DE69942444D1 - Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen - Google Patents

Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen

Info

Publication number
DE69942444D1
DE69942444D1 DE69942444T DE69942444T DE69942444D1 DE 69942444 D1 DE69942444 D1 DE 69942444D1 DE 69942444 T DE69942444 T DE 69942444T DE 69942444 T DE69942444 T DE 69942444T DE 69942444 D1 DE69942444 D1 DE 69942444D1
Authority
DE
Germany
Prior art keywords
fluorescer
fluorescence
allelic
target sequence
allelen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69942444T
Other languages
English (en)
Inventor
Kenneth J Livak
Federico Goodsaid
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of DE69942444D1 publication Critical patent/DE69942444D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
DE69942444T 1998-02-04 1999-01-08 Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen Expired - Lifetime DE69942444D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1859598A 1998-02-04 1998-02-04
PCT/US1999/000499 WO1999040226A2 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Publications (1)

Publication Number Publication Date
DE69942444D1 true DE69942444D1 (de) 2010-07-15

Family

ID=21788757

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69942444T Expired - Lifetime DE69942444D1 (de) 1998-02-04 1999-01-08 Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen

Country Status (8)

Country Link
US (5) US6154707A (de)
EP (1) EP1053348B1 (de)
JP (1) JP4388694B2 (de)
AT (1) ATE469982T1 (de)
AU (1) AU758463B2 (de)
CA (1) CA2318880C (de)
DE (1) DE69942444D1 (de)
WO (1) WO1999040226A2 (de)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69737883T2 (de) 1996-04-25 2008-03-06 Bioarray Solutions Ltd. Licht-regulierte, elektrokinetische zusammensetzung von partikeln an oberflächen
AU741141B2 (en) * 1997-11-04 2001-11-22 Roche Diagnostics Gmbh Specific and sensitive method for detecting nucleic acids
JP4388694B2 (ja) 1998-02-04 2009-12-24 アプライド バイオシステムズ， エルエルシー 複数のアレル部位における増幅産物のゲノム型決定
US7662550B1 (en) 1999-10-22 2010-02-16 Phri Properties, Inc. Assays for short sequence variants
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US20040081959A9 (en) 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
JP2001299392A (ja) * 2000-04-25 2001-10-30 Otsuka Pharmaceut Co Ltd Ｃａｔｃｈ２２症候群検出方法
US7435541B2 (en) 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
WO2001090419A2 (en) * 2000-05-23 2001-11-29 Variagenics, Inc. Methods for genetic analysis of dna to detect sequence variances
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
JP4744778B2 (ja) 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド 特定のランダム粒子アレイを適用した複数の被検体分子の分析方法
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
KR100888377B1 (ko) * 2001-06-28 2009-03-13 노바티스 백신즈 앤드 다이아그노스틱스 인코포레이티드 파르보바이러스 ｂ19의 진단 분석
JP4377689B2 (ja) 2001-10-15 2009-12-02 バイオアレイ ソリューションズ リミテッド 同時尋問及び酵素仲介検出による多型遺伝子座の複合分析
CN1483076A (zh) * 2001-10-26 2004-03-17 松下电器产业株式会社 靶核酸的检测方法及核酸探针
US20110111389A1 (en) 2001-11-07 2011-05-12 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis for human papillomavirus and the device thereof
US7732138B2 (en) * 2001-11-07 2010-06-08 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis and the device thereof
US20050079493A1 (en) * 2002-11-09 2005-04-14 Tam Joseph Wing On DNA fingerprinting using allelic specific oligonucleotide reversed dot blot (ASO-RDB) flow through hybridization process and device
AU2003216489A1 (en) * 2002-03-05 2003-09-22 M.C.W Research Foundation, Inc. Methods and compositions for pharmacological and toxicological evaluation of test agents
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1509624A2 (de) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Methode für den nukleinsäurenachweis basierend auf met/fret wobei sich die fluorophoren gruppen auf komplementären strängen befinden
AU2003901871A0 (en) * 2003-03-31 2003-05-08 Vision Biosystems Limited A method and apparatus for fluid dispensation, preparation and dilation
CA2492064C (en) 2002-06-20 2016-03-15 Vision Biosystems Limited Biological reaction apparatus with draining mechanism
WO2004018626A2 (en) * 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
AU2003298655A1 (en) 2002-11-15 2004-06-15 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
WO2004055709A2 (en) * 2002-12-13 2004-07-01 Applera Corporation Methods for identifying, viewing, and analyzing syntenic and orthologous genomic regions between two or more species
US7851150B2 (en) 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
CA2512110A1 (en) 2002-12-31 2004-07-22 Mmi Genomics, Inc. Compositions, methods, and systems for inferring bovine breed
US7858301B2 (en) * 2003-05-07 2010-12-28 Bioarray Solutions, Ltd. Method of probe design for nucleic acid analysis by multiplexed hybridization
US20050089889A1 (en) * 2003-06-20 2005-04-28 Ramsing Niels B. Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
US20050038776A1 (en) * 2003-08-15 2005-02-17 Ramin Cyrus Information system for biological and life sciences research
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20050221357A1 (en) * 2003-09-19 2005-10-06 Mark Shannon Normalization of gene expression data
NZ546072A (en) 2003-09-22 2009-08-28 Bioarray Solutions Ltd Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US7332280B2 (en) * 2003-10-14 2008-02-19 Ronald Levy Classification of patients having diffuse large B-cell lymphoma based upon gene expression
WO2005042763A2 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
EP1694859B1 (de) 2003-10-29 2015-01-07 Bioarray Solutions Ltd Multiplex-nukleinsäureanalyse mittels fragmentierung doppelsträngiger dna
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
EP1561823A1 (de) * 2004-02-04 2005-08-10 Biotez Berlin-Buch GmbH Verfahren zum Nachweis von Einzelnukleotid-polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
ATE494392T1 (de) * 2004-09-21 2011-01-15 Life Technologies Corp Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
US20060111915A1 (en) * 2004-11-23 2006-05-25 Applera Corporation Hypothesis generation
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
US20080213762A1 (en) * 2004-12-08 2008-09-04 Takeshi Yamamoto Method of Gene Sequence Examination
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
EP1907560B1 (de) 2005-05-20 2013-01-23 Integrated DNA Technologies, Inc. Verbindungen und verfahren zur markierung von oligonukleotiden
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
WO2007044091A2 (en) 2005-06-02 2007-04-19 Fluidigm Corporation Analysis using microfluidic partitioning devices
EP2660482B1 (de) * 2005-08-22 2019-08-07 Life Technologies Corporation Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen
AU2007260676A1 (en) * 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
WO2007147074A2 (en) 2006-06-14 2007-12-21 Living Microsystems, Inc. Use of highly parallel snp genotyping for fetal diagnosis
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
WO2007147018A1 (en) * 2006-06-14 2007-12-21 Cellpoint Diagnostics, Inc. Analysis of rare cell-enriched samples
US8372584B2 (en) * 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
ES2327956T3 (es) * 2006-10-12 2009-11-05 Bio-Rad Pasteur Sondas de doble hebra para la deteccion de acidos nucleicos, mediante fluorescencia.
DE102007013099A1 (de) * 2007-03-14 2008-09-18 Aj Innuscreen Gmbh Verfahren und Testkit zum schnellen Nachweis spezifischer Nukleinsäuresequenzen, insbesondere zum Nachweis von Mutationen oder SNP's
EP2116614A1 (de) * 2008-05-06 2009-11-11 Qiagen GmbH Gleichzeitiger Nachweis mehrerer Nukleinsäuresequenzen in einer Reaktion
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
ES2575005T3 (es) * 2008-06-13 2016-06-23 Codexis, Inc. Método de síntesis de variantes de polinucleótidos
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
CA2737643C (en) 2008-09-20 2020-10-06 Hei-Mun Fan Noninvasive diagnosis of fetal aneuploidy by sequencing
EP2419729A4 (de) * 2009-04-13 2015-11-25 Canon Us Life Sciences Inc Schnelles verfahren für mustererkennung, maschinentraining und automatisierte genotypenklassifizierung durch korrelationsanalyse dynamischer signale
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
JP5886828B2 (ja) 2010-03-26 2016-03-16 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド 核酸のハイブリダイゼーションを強化する方法
EP2896696B1 (de) 2010-09-07 2017-12-27 Integrated Dna Technologies, Inc. Modifikationen für Antisense-Verbindungen
US20120083035A1 (en) 2010-09-30 2012-04-05 Dharmacon, Inc. Modified Cell Lines for Increasing Lentiviral Titers
WO2012046140A1 (en) * 2010-10-05 2012-04-12 Finnzymes Oy Enzyme mixture
SG11201407901PA (en) 2012-05-21 2015-01-29 Fluidigm Corp Single-particle analysis of particle populations
RU2757416C2 (ru) * 2016-04-06 2021-10-15 Лайф Текнолоджис Корпорейшн Композиции, способы и наборы для синтеза и обнаружения нуклеиновых кислот
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックＩｐマネジメント株式会社 核酸検出用オリゴヌクレオチド
CN116981781A (zh) * 2021-01-15 2023-10-31 阅尔基因美国公司 用于高度多重定量pcr的循环多重分析

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
WO1992001814A2 (en) * 1990-07-24 1992-02-06 F. Hoffmann-La-Roche Ag The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5403707A (en) * 1993-05-14 1995-04-04 Eastman Kodak Company Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5834181A (en) * 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
DE69535882D1 (de) * 1994-12-30 2008-12-18 Univ Georgetown Fluoreszenztest zum nachweis der spaltung einer nukleinsäure
DE19524579C2 (de) * 1995-07-06 1997-12-18 Daimler Benz Ag Transformator als Strombegrenzer
US5792610A (en) * 1996-05-01 1998-08-11 Biorad Laboratories, Inc. Method for conducting multiparametric fluorescence in situ hybridization
US5759781A (en) * 1995-12-22 1998-06-02 Yale University Multiparametric fluorescence in situ hybridization
JP3898228B2 (ja) * 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
US6015667A (en) * 1996-06-03 2000-01-18 The Perkin-Emer Corporation Multicomponent analysis method including the determination of a statistical confidence interval
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
JP4388694B2 (ja) 1998-02-04 2009-12-24 アプライド バイオシステムズ， エルエルシー 複数のアレル部位における増幅産物のゲノム型決定
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification

Also Published As

Publication number Publication date
CA2318880A1 (en) 1999-08-12
AU2314499A (en) 1999-08-23
US6884583B2 (en) 2005-04-26
EP1053348B1 (de) 2010-06-02
US7132239B2 (en) 2006-11-07
WO1999040226A2 (en) 1999-08-12
CA2318880C (en) 2010-07-27
US20040053302A1 (en) 2004-03-18
AU758463B2 (en) 2003-03-20
US6154707A (en) 2000-11-28
US20080187913A1 (en) 2008-08-07
ATE469982T1 (de) 2010-06-15
JP2002502615A (ja) 2002-01-29
WO1999040226A3 (en) 2000-08-17
EP1053348A2 (de) 2000-11-22
US20020164630A1 (en) 2002-11-07
JP4388694B2 (ja) 2009-12-24
US20120053069A1 (en) 2012-03-01

Similar Documents

Publication Publication Date Title
DE69942444D1 (de) Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
Sharkey et al. Detection and quantification of gene expression in environmental bacteriology
Isacsson et al. Rapid and specific detection of PCR products using light-up probes
Andersson et al. Properties of targeted preamplification in DNA and cDNA quantification
DE602005027067D1 (de) Nachweis chromosomaler störungen
HUP0101385A2 (hu) Eljárás nukleinsavak detektálására PCR-módszerrel
RU2012129362A (ru) Обнаружение мишени tsg праймером
DE3751423D1 (de) Primers und Sonden zum Nachweis von Nukleinsäuren.
WO2005047533B1 (en) Method of detecting mutations associated with thrombosis
NO20052692L (no) Fremgangsmate for a pavise genmutasjon.
EP1241266A4 (de) Methode zum nachweis von nukleinsäurepolymorphismen
ATE288964T1 (de) Methoden für externe kontrollen zur nukleinsäure amplifizierung
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
CA2319662A1 (en) Modified nucleic acid probes and uses thereof
KR930004477A (ko) 미코박테리아 프라이머 및 탐침
DE60332775D1 (de) Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen
Wang et al. DNAzyme‐Based Probes for Telomerase Detection in Early‐Stage Cancer Diagnosis
WO2005047468A8 (en) Improved methods for detecting and measuring specific nucleic acid sequences
Nikiforov Fluorogenic polymerase, endonuclease, and ligase assays based on DNA substrates labeled with a single fluorophore
WO2003002762A3 (en) Enhanced detection and distinction of differential gene expression by enzymatic probe ligation and amplification
JP7391321B2 (ja) アクネ菌のｄｎａタイプの判別用オリゴヌクレオチドセット、キット及びアクネ菌のｄｎａタイプの判別方法
ATE315103T1 (de) Verfahren zur typisierung des ha-1 nebenhistokompatibilitätsantigens
ATE412068T1 (de) Spezifische multiplex-analyse von nukleinsäuren
Holden et al. Quantitative real-time PCR: fluorescent probe options and issues
US20070202521A1 (en) Single Molecule DNA Sequencing Using Fret Based Dynamic Labeling