DE69942444D1 - Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen - Google Patents

Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen

Info

Publication number
DE69942444D1
DE69942444D1 DE69942444T DE69942444T DE69942444D1 DE 69942444 D1 DE69942444 D1 DE 69942444D1 DE 69942444 T DE69942444 T DE 69942444T DE 69942444 T DE69942444 T DE 69942444T DE 69942444 D1 DE69942444 D1 DE 69942444D1
Authority
DE
Germany
Prior art keywords
fluorescer
fluorescence
allelic
target sequence
allelen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69942444T
Other languages
English (en)
Inventor
Kenneth J Livak
Federico Goodsaid
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of DE69942444D1 publication Critical patent/DE69942444D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
DE69942444T 1998-02-04 1999-01-08 Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen Expired - Lifetime DE69942444D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1859598A 1998-02-04 1998-02-04
PCT/US1999/000499 WO1999040226A2 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Publications (1)

Publication Number Publication Date
DE69942444D1 true DE69942444D1 (de) 2010-07-15

Family

ID=21788757

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69942444T Expired - Lifetime DE69942444D1 (de) 1998-02-04 1999-01-08 Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen

Country Status (8)

Country Link
US (5) US6154707A (de)
EP (1) EP1053348B1 (de)
JP (1) JP4388694B2 (de)
AT (1) ATE469982T1 (de)
AU (1) AU758463B2 (de)
CA (1) CA2318880C (de)
DE (1) DE69942444D1 (de)
WO (1) WO1999040226A2 (de)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2288760T3 (es) 1996-04-25 2008-01-16 Bioarray Solutions Ltd. Ensamblaje electrocinetico controlado por luz de particulas proximas a superficies.
JP2002509694A (ja) * 1997-11-04 2002-04-02 ロシュ ダイアグノスティックス ゲーエムベーハー 特異的かつ高感度な核酸検出方法
CA2318880C (en) * 1998-02-04 2010-07-27 Perkin-Elmer Corporation Determination of a genotype of an amplification product at multiple allelic sites
WO2001031062A1 (en) 1999-10-22 2001-05-03 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US20040081959A9 (en) 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
JP2001299392A (ja) * 2000-04-25 2001-10-30 Otsuka Pharmaceut Co Ltd Catch22症候群検出方法
AU2001264811A1 (en) * 2000-05-23 2001-12-03 Vincent P. Stanton Jr. Methods for genetic analysis of dna to detect sequence variances
US7435541B2 (en) 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
DE60117556T2 (de) 2000-06-21 2006-11-02 Bioarray Solutions Ltd. Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
KR100888377B1 (ko) * 2001-06-28 2009-03-13 노바티스 백신즈 앤드 다이아그노스틱스 인코포레이티드 파르보바이러스 b19의 진단 분석
ES2661167T3 (es) 2001-10-15 2018-03-27 Bioarray Solutions Ltd. Análisis multiplexado de loci polimórficos mediante consulta simultánea y detección mediada por enzimas
WO2003035864A1 (fr) * 2001-10-26 2003-05-01 Matsushita Electric Industrial Co., Ltd. Methode de detection d'acide nucleique cible et sonde d'acide nucleique
US7732138B2 (en) * 2001-11-07 2010-06-08 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis and the device thereof
US20110111389A1 (en) 2001-11-07 2011-05-12 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis for human papillomavirus and the device thereof
US20050079493A1 (en) * 2002-11-09 2005-04-14 Tam Joseph Wing On DNA fingerprinting using allelic specific oligonucleotide reversed dot blot (ASO-RDB) flow through hybridization process and device
ATE406093T1 (de) * 2002-03-05 2008-09-15 Mcw Res Found Inc Verfahren und zusammensetzungen für die pharmakologische und toxikologische auswertung von testsubstanzen
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1509624A2 (de) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Methode für den nukleinsäurenachweis basierend auf met/fret wobei sich die fluorophoren gruppen auf komplementären strängen befinden
AU2003901871A0 (en) * 2003-03-31 2003-05-08 Vision Biosystems Limited A method and apparatus for fluid dispensation, preparation and dilation
JP2005530165A (ja) * 2002-06-20 2005-10-06 ビジョン・バイオシステムズ・リミテッド 流体排出機構を備えた生物反応装置
EP1543154A4 (de) * 2002-08-21 2006-08-16 Epoch Biosciences Inc Assay mit endonuklease für abasische stellen
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
US20050066276A1 (en) * 2002-12-13 2005-03-24 Moore Helen M. Methods for identifying, viewing, and analyzing syntenic and orthologous genomic regions between two or more species
US7851150B2 (en) 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
US20050260603A1 (en) 2002-12-31 2005-11-24 Mmi Genomics, Inc. Compositions for inferring bovine traits
US7858301B2 (en) * 2003-05-07 2010-12-28 Bioarray Solutions, Ltd. Method of probe design for nucleic acid analysis by multiplexed hybridization
ATE553216T1 (de) 2003-06-20 2012-04-15 Exiqon As Sonden, bibliotheken und kits zur analyse von nukleinsäuregemischen und verfahren zu deren konstruktion
US20050038776A1 (en) * 2003-08-15 2005-02-17 Ramin Cyrus Information system for biological and life sciences research
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20050221357A1 (en) * 2003-09-19 2005-10-06 Mark Shannon Normalization of gene expression data
AU2004276761B2 (en) 2003-09-22 2009-12-24 Bioarray Solutions, Ltd. Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US7332280B2 (en) * 2003-10-14 2008-02-19 Ronald Levy Classification of patients having diffuse large B-cell lymphoma based upon gene expression
AU2004286252A1 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
AU2004287069B2 (en) 2003-10-29 2009-07-16 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded DNA
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
EP1561823A1 (de) * 2004-02-04 2005-08-10 Biotez Berlin-Buch GmbH Verfahren zum Nachweis von Einzelnukleotid-polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP2186908A1 (de) * 2004-09-21 2010-05-19 Applied Biosystems, LLC Zweifarbige Echtzeit/Endpunkt-Quantifizierung von Mikro-RNAs (miRNAs)
US20060111915A1 (en) * 2004-11-23 2006-05-25 Applera Corporation Hypothesis generation
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
JP4190562B2 (ja) * 2004-12-08 2008-12-03 健 山本 遺伝子配列検査法
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
AU2006251637B2 (en) 2005-05-20 2012-06-14 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
WO2007044091A2 (en) 2005-06-02 2007-04-19 Fluidigm Corporation Analysis using microfluidic partitioning devices
WO2007024778A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Device, system and method for depositing processed immiscible-fluid-discrete-volumes
WO2007147018A1 (en) * 2006-06-14 2007-12-21 Cellpoint Diagnostics, Inc. Analysis of rare cell-enriched samples
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
EP4108780A1 (de) * 2006-06-14 2022-12-28 Verinata Health, Inc. Analyse seltener zellen mittels probentrennung und dna-etiketten
EP2589668A1 (de) 2006-06-14 2013-05-08 Verinata Health, Inc Analyse seltener Zellen mittels Probentrennung und DNA-Etiketten
EP2029779A4 (de) 2006-06-14 2010-01-20 Living Microsystems Inc Verwendung hoch paralleler snp-genotypisierung zur fötalen diagnose
DE602006008150D1 (de) * 2006-10-12 2009-09-10 Bio Rad Pasteur Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren
DE102007013099A1 (de) * 2007-03-14 2008-09-18 Aj Innuscreen Gmbh Verfahren und Testkit zum schnellen Nachweis spezifischer Nukleinsäuresequenzen, insbesondere zum Nachweis von Mutationen oder SNP's
EP2336358A1 (de) * 2008-05-06 2011-06-22 QIAGEN GmbH Gleichzeitiger Nachweis mehrerer Nukleinsäuresequenzen in einer Reaktion
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
ES2705694T3 (es) * 2008-06-13 2019-03-26 Codexis Inc Método de síntesis de variantes de polinucleótidos
EP2952589B1 (de) 2008-09-20 2018-02-14 The Board of Trustees of The Leland Stanford Junior University Nicht invasive diagnose von fötaler aneuploidie durch sequenzierung
US8412466B2 (en) * 2009-04-13 2013-04-02 Canon U.S. Life Sciences, Inc. Rapid method of pattern recognition, machine learning, and automated genotype classification through correlation analysis of dynamic signals
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
US20120108799A1 (en) 2010-09-07 2012-05-03 Integrated Dna Technologies, Inc. Modifications for Antisense Compounds
US20120083035A1 (en) 2010-09-30 2012-04-05 Dharmacon, Inc. Modified Cell Lines for Increasing Lentiviral Titers
WO2012046140A1 (en) * 2010-10-05 2012-04-12 Finnzymes Oy Enzyme mixture
WO2013177206A2 (en) 2012-05-21 2013-11-28 Fluidigm Corporation Single-particle analysis of particle populations
EP3440221A1 (de) * 2016-04-06 2019-02-13 Life Technologies Corporation Zusammensetzungen, verfahren und kits zur synthese und zum nachweis von nukleinsäuren
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックIpマネジメント株式会社 核酸検出用オリゴヌクレオチド
CN116981781A (zh) * 2021-01-15 2023-10-31 阅尔基因美国公司 用于高度多重定量pcr的循环多重分析

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
DK0540693T3 (da) * 1990-07-24 1999-09-13 Hoffmann La Roche Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5403707A (en) * 1993-05-14 1995-04-04 Eastman Kodak Company Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5834181A (en) * 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
WO1996021144A1 (en) * 1994-12-30 1996-07-11 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
DE19524579C2 (de) * 1995-07-06 1997-12-18 Daimler Benz Ag Transformator als Strombegrenzer
US5792610A (en) * 1996-05-01 1998-08-11 Biorad Laboratories, Inc. Method for conducting multiparametric fluorescence in situ hybridization
US5759781A (en) * 1995-12-22 1998-06-02 Yale University Multiparametric fluorescence in situ hybridization
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US6015667A (en) * 1996-06-03 2000-01-18 The Perkin-Emer Corporation Multicomponent analysis method including the determination of a statistical confidence interval
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
CA2318880C (en) 1998-02-04 2010-07-27 Perkin-Elmer Corporation Determination of a genotype of an amplification product at multiple allelic sites
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification

Also Published As

Publication number Publication date
CA2318880C (en) 2010-07-27
US6884583B2 (en) 2005-04-26
JP2002502615A (ja) 2002-01-29
EP1053348A2 (de) 2000-11-22
AU758463B2 (en) 2003-03-20
US7132239B2 (en) 2006-11-07
ATE469982T1 (de) 2010-06-15
US20080187913A1 (en) 2008-08-07
US20040053302A1 (en) 2004-03-18
CA2318880A1 (en) 1999-08-12
US20020164630A1 (en) 2002-11-07
US6154707A (en) 2000-11-28
EP1053348B1 (de) 2010-06-02
JP4388694B2 (ja) 2009-12-24
US20120053069A1 (en) 2012-03-01
AU2314499A (en) 1999-08-23
WO1999040226A3 (en) 2000-08-17
WO1999040226A2 (en) 1999-08-12

Similar Documents

Publication Publication Date Title
DE69942444D1 (de) Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
Leutenegger et al. Quantitative real-time PCR for the measurement of feline cytokine mRNA
Sharkey et al. Detection and quantification of gene expression in environmental bacteriology
Isacsson et al. Rapid and specific detection of PCR products using light-up probes
Andersson et al. Properties of targeted preamplification in DNA and cDNA quantification
DE602005027067D1 (de) Nachweis chromosomaler störungen
HUP0101385A2 (hu) Eljárás nukleinsavak detektálására PCR-módszerrel
RU2012129362A (ru) Обнаружение мишени tsg праймером
DE3751423T2 (de) Primers und Sonden zum Nachweis von Nukleinsäuren.
WO2005047533B1 (en) Method of detecting mutations associated with thrombosis
NO20052692L (no) Fremgangsmate for a pavise genmutasjon.
EP1241266A4 (de) Methode zum nachweis von nukleinsäurepolymorphismen
ATE288964T1 (de) Methoden für externe kontrollen zur nukleinsäure amplifizierung
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
CA2319662A1 (en) Modified nucleic acid probes and uses thereof
KR930004477A (ko) 미코박테리아 프라이머 및 탐침
DE60309817D1 (de) Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen
Wang et al. DNAzyme‐Based Probes for Telomerase Detection in Early‐Stage Cancer Diagnosis
WO2005047468A3 (en) Improved methods for detecting and measuring specific nucleic acid sequences
Nikiforov Fluorogenic polymerase, endonuclease, and ligase assays based on DNA substrates labeled with a single fluorophore
WO2003002762A3 (en) Enhanced detection and distinction of differential gene expression by enzymatic probe ligation and amplification
JP7391321B2 (ja) アクネ菌のdnaタイプの判別用オリゴヌクレオチドセット、キット及びアクネ菌のdnaタイプの判別方法
ATE315103T1 (de) Verfahren zur typisierung des ha-1 nebenhistokompatibilitätsantigens
ATE412068T1 (de) Spezifische multiplex-analyse von nukleinsäuren
Holden et al. Quantitative real-time PCR: fluorescent probe options and issues