EP1127619A3 - Improvements in thermal cycler for PCR - Google Patents

Improvements in thermal cycler for PCR Download PDF

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Publication number
EP1127619A3
EP1127619A3 EP01112394A EP01112394A EP1127619A3 EP 1127619 A3 EP1127619 A3 EP 1127619A3 EP 01112394 A EP01112394 A EP 01112394A EP 01112394 A EP01112394 A EP 01112394A EP 1127619 A3 EP1127619 A3 EP 1127619A3
Authority
EP
European Patent Office
Prior art keywords
sample block
heatsink
pcr
thermal cycler
close contact
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP01112394A
Other languages
German (de)
French (fr)
Other versions
EP1127619B1 (en
EP1127619A2 (en
Inventor
John G. Atwood
Adrian Fawcett
Keith S. Ferrara
Paul M. Hetherington
Richard W. Noreiks
Douglas E. Olsen
John R. Widomski
Charles M. Wittmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Applied Biosystems Inc
Original Assignee
PE Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP09013036.0A priority Critical patent/EP2156892B1/en
Priority to EP14198058.1A priority patent/EP2913109A1/en
Priority to EP03022672A priority patent/EP1386666B1/en
Priority to EP09006229A priority patent/EP2090366B1/en
Priority to EP09016131.6A priority patent/EP2186568B1/en
Application filed by PE Corp filed Critical PE Corp
Priority claimed from EP98913265A external-priority patent/EP0963250B1/en
Publication of EP1127619A2 publication Critical patent/EP1127619A2/en
Publication of EP1127619A3 publication Critical patent/EP1127619A3/en
Application granted granted Critical
Publication of EP1127619B1 publication Critical patent/EP1127619B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater

Abstract

An assembly for cycling vials of reaction mixtures through a series of temperature excursions, comprising:
  • a sample block (36) for receiving vials of reaction mixtures;
  • a plurality of thermoelectric devices (60);
  • a heatsink (34);
  • a clamping mechanism positioned so as to clamp said thermoelectric devices between said sample block and said heatsink;
  • a heater (74) positioned around the perimeter of said sample block; and
  • a pin (24) having a first end and a second end, said first end in close contact with said sample block and said second end in close contact with said heatsink so as to provide a thermal path between said sample block and said heatsink.
    • Figure 00000001
    EP01112394A 1997-03-28 1998-03-30 Assembly for thermal cycler for PCR Expired - Lifetime EP1127619B1 (en)

    Priority Applications (5)

    Application Number Priority Date Filing Date Title
    EP14198058.1A EP2913109A1 (en) 1997-03-28 1998-03-30 Assembly for thermal cycler for PCR
    EP03022672A EP1386666B1 (en) 1997-03-28 1998-03-30 Improvements in thermal cycler for pcr
    EP09006229A EP2090366B1 (en) 1997-03-28 1998-03-30 Improvements in thermal cycler for PCR
    EP09016131.6A EP2186568B1 (en) 1997-03-28 1998-03-30 Assembly for thermal cycler for PCR
    EP09013036.0A EP2156892B1 (en) 1997-03-28 1998-03-30 Thermal cycler for PCR

    Applications Claiming Priority (5)

    Application Number Priority Date Filing Date Title
    US4175497P 1997-03-28 1997-03-28
    US41754P 1997-03-28
    US4612297P 1997-05-09 1997-05-09
    US46122P 1997-05-09
    EP98913265A EP0963250B1 (en) 1997-03-28 1998-03-30 Improvements in thermal cycler for pcr

    Related Parent Applications (1)

    Application Number Title Priority Date Filing Date
    EP98913265A Division EP0963250B1 (en) 1997-03-28 1998-03-30 Improvements in thermal cycler for pcr

    Related Child Applications (3)

    Application Number Title Priority Date Filing Date
    EP03022672A Division EP1386666B1 (en) 1997-03-28 1998-03-30 Improvements in thermal cycler for pcr
    EP14198058.1A Division EP2913109A1 (en) 1997-03-28 1998-03-30 Assembly for thermal cycler for PCR
    EP09016131.6A Division EP2186568B1 (en) 1997-03-28 1998-03-30 Assembly for thermal cycler for PCR

    Publications (3)

    Publication Number Publication Date
    EP1127619A2 EP1127619A2 (en) 2001-08-29
    EP1127619A3 true EP1127619A3 (en) 2001-09-05
    EP1127619B1 EP1127619B1 (en) 2003-10-08

    Family

    ID=27239812

    Family Applications (1)

    Application Number Title Priority Date Filing Date
    EP01112394A Expired - Lifetime EP1127619B1 (en) 1997-03-28 1998-03-30 Assembly for thermal cycler for PCR

    Country Status (1)

    Country Link
    EP (1) EP1127619B1 (en)

    Families Citing this family (4)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    DE10228431B4 (en) * 2002-06-26 2004-08-26 Eppendorf Ag Laboratory sample temperature control device with recordings
    US20040072334A1 (en) * 2002-10-15 2004-04-15 The Regents Of The University Of California Thermal cycler
    WO2007149696A1 (en) † 2006-06-23 2007-12-27 Applera Corporation Systems and methods for cooling in biological analysis instruments
    EP2198968B1 (en) 2008-12-16 2018-10-17 F. Hoffmann-La Roche AG Systems and methods for monitoring a thermoelectric heating and cooling device

    Citations (4)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    US4865986A (en) * 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
    EP0488769A2 (en) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
    EP0642831A1 (en) * 1993-09-10 1995-03-15 F. Hoffmann-La Roche Ag Device for automatically carrying out temperature cycling
    US5410130A (en) * 1994-04-20 1995-04-25 Ericomp, Inc. Heating and temperature cycling

    Patent Citations (4)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    US4865986A (en) * 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
    EP0488769A2 (en) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
    EP0642831A1 (en) * 1993-09-10 1995-03-15 F. Hoffmann-La Roche Ag Device for automatically carrying out temperature cycling
    US5410130A (en) * 1994-04-20 1995-04-25 Ericomp, Inc. Heating and temperature cycling

    Also Published As

    Publication number Publication date
    EP1127619B1 (en) 2003-10-08
    EP1127619A2 (en) 2001-08-29

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