EP1231260A3 - A method for isolating cells that express FLK-2 receptors on their surface - Google Patents

A method for isolating cells that express FLK-2 receptors on their surface Download PDF

Info

Publication number
EP1231260A3
EP1231260A3 EP02004000A EP02004000A EP1231260A3 EP 1231260 A3 EP1231260 A3 EP 1231260A3 EP 02004000 A EP02004000 A EP 02004000A EP 02004000 A EP02004000 A EP 02004000A EP 1231260 A3 EP1231260 A3 EP 1231260A3
Authority
EP
European Patent Office
Prior art keywords
receptor protein
protein tyrosine
nucleic acid
flk
receptors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP02004000A
Other languages
German (de)
French (fr)
Other versions
EP1231260B1 (en
EP1231260A2 (en
Inventor
Ihor R. Lemischka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of EP1231260A2 publication Critical patent/EP1231260A2/en
Publication of EP1231260A3 publication Critical patent/EP1231260A3/en
Application granted granted Critical
Publication of EP1231260B1 publication Critical patent/EP1231260B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02—Acyclic radicals, not substituted by cyclic structures
    • C07H15/12—Acyclic radicals, not substituted by cyclic structures attached to a nitrogen atom of the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinase expressed in primitive hematopoietic cells and not expressed in mature hemotopoietic cells are provided. Also included are the receptors encoded by such nucleic acid molecules; the nucleic acid molecules encoding receptor protein tyrosine kinases having the sequences shown in Figure 1 (flk-2); the receptor protein tyrosine kinases having the amino acid sequences shown in Figure 1 (flk-2); ligands for the receptors; nucleic acid sequences that encode the ligands; methods of stimulating the proliferation and/or differentiation of primitive mammalian hematopoietic stem cells comprising contacting the stem cells with a ligand that binds to a receptor protein tyrosine kinase expressed in primitive mammalian hematopoietic cells and not expressed in mature hematopoietic cells and antibodies to the claimed receptor protein tyrosine kinase flk-2.
EP02004000A 1991-04-02 1992-04-02 A method for isolating cells that express FLK-2 receptors on their surface Expired - Lifetime EP1231260B1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US679666 1984-12-07
US67966691A 1991-04-02 1991-04-02
US72891391A 1991-06-28 1991-06-28
US728913 1991-06-28
US79306591A 1991-11-15 1991-11-15
US793065 1991-11-15
US07/813,593 US5185438A (en) 1991-04-02 1991-12-24 Nucleic acids encoding hencatoporetic stem cell receptor flk-2
EP92911275A EP0580760B1 (en) 1991-04-02 1992-04-02 Antibodies of totipotent hematopoietic stem cell receptors flk-1
US813593 2001-03-21

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
EP92911275A Division EP0580760B1 (en) 1991-04-02 1992-04-02 Antibodies of totipotent hematopoietic stem cell receptors flk-1

Publications (3)

Publication Number Publication Date
EP1231260A2 EP1231260A2 (en) 2002-08-14
EP1231260A3 true EP1231260A3 (en) 2003-12-03
EP1231260B1 EP1231260B1 (en) 2008-09-17

Family

ID=27505386

Family Applications (6)

Application Number Title Priority Date Filing Date
EP98119396A Expired - Lifetime EP0909813B1 (en) 1991-04-02 1992-04-02 Totipotent hematopoietic stem cell receptor flk-2
EP92911275A Expired - Lifetime EP0580760B1 (en) 1991-04-02 1992-04-02 Antibodies of totipotent hematopoietic stem cell receptors flk-1
EP02004002A Expired - Lifetime EP1231265B1 (en) 1991-04-02 1992-04-02 A receptor protein tyrosine kinase (FLK-1)
EP02004001A Expired - Lifetime EP1231261B1 (en) 1991-04-02 1992-04-02 A method for isolating cells that express FLK-1 receptors on their surface
EP02004000A Expired - Lifetime EP1231260B1 (en) 1991-04-02 1992-04-02 A method for isolating cells that express FLK-2 receptors on their surface
EP07001970A Withdrawn EP1808445A3 (en) 1991-04-02 1992-04-02 A receptor protein tyrosine kinase

Family Applications Before (4)

Application Number Title Priority Date Filing Date
EP98119396A Expired - Lifetime EP0909813B1 (en) 1991-04-02 1992-04-02 Totipotent hematopoietic stem cell receptor flk-2
EP92911275A Expired - Lifetime EP0580760B1 (en) 1991-04-02 1992-04-02 Antibodies of totipotent hematopoietic stem cell receptors flk-1
EP02004002A Expired - Lifetime EP1231265B1 (en) 1991-04-02 1992-04-02 A receptor protein tyrosine kinase (FLK-1)
EP02004001A Expired - Lifetime EP1231261B1 (en) 1991-04-02 1992-04-02 A method for isolating cells that express FLK-1 receptors on their surface

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP07001970A Withdrawn EP1808445A3 (en) 1991-04-02 1992-04-02 A receptor protein tyrosine kinase

Country Status (7)

Country Link
US (3) US5185438A (en)
EP (6) EP0909813B1 (en)
KR (3) KR100261465B1 (en)
AU (1) AU1924892A (en)
CA (1) CA2107463C (en)
DE (5) DE69232687T2 (en)
WO (2) WO1992017486A1 (en)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030206899A1 (en) * 1991-03-29 2003-11-06 Genentech, Inc. Vascular endothelial cell growth factor antagonists
US6582959B2 (en) * 1991-03-29 2003-06-24 Genentech, Inc. Antibodies to vascular endothelial cell growth factor
US5270458A (en) * 1991-04-02 1993-12-14 The Trustees Of Princeton University Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2
US5367057A (en) 1991-04-02 1994-11-22 The Trustees Of Princeton University Tyrosine kinase receptor flk-2 and fragments thereof
US5185438A (en) * 1991-04-02 1993-02-09 The Trustees Of Princeton University Nucleic acids encoding hencatoporetic stem cell receptor flk-2
US6331302B1 (en) * 1992-01-22 2001-12-18 Genentech, Inc. Protein tyrosine kinase agonist antibodies
US5861301A (en) * 1992-02-20 1999-01-19 American Cayanamid Company Recombinant kinase insert domain containing receptor and gene encoding same
US6228609B1 (en) * 1992-07-09 2001-05-08 Systemix, Inc. Soluble Flk-2 sequence
US6824777B1 (en) * 1992-10-09 2004-11-30 Licentia Ltd. Flt4 (VEGFR-3) as a target for tumor imaging and anti-tumor therapy
US5776755A (en) * 1992-10-09 1998-07-07 Helsinki University Licensing, Ltd. FLT4, a receptor tyrosine kinase
US6107046A (en) * 1992-10-09 2000-08-22 Orion Corporation Antibodies to Flt4, a receptor tyrosine kinase and uses thereof
US6177401B1 (en) 1992-11-13 2001-01-23 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften Use of organic compounds for the inhibition of Flk-1 mediated vasculogenesis and angiogenesis
US6872699B2 (en) 1992-11-13 2005-03-29 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften, E.V. Truncated Flk-1 receptor protein, methods of use and a recombinant vector containing a nucleotide encoding the truncated Flk-1 protein
US5792771A (en) * 1992-11-13 1998-08-11 Sugen, Inc. Quinazoline compounds and compositions thereof for the treatment of disease
US5981569A (en) * 1992-11-13 1999-11-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Substituted phenylacrylonitrile compounds and compositions thereof for the treatment of disease
CN1173991C (en) * 1992-11-13 2004-11-03 马克斯普朗克科学促进协会 Fik-1 as receiptor of growth factor of blood vessel inner-skin
US5712395A (en) * 1992-11-13 1998-01-27 Yissum Research Development Corp. Compounds for the treatment of disorders related to vasculogenesis and/or angiogenesis
US5763441A (en) * 1992-11-13 1998-06-09 Sugen, Inc. Compounds for the treatment of disorders related to vasculogenesis and/or angiogenesis
WO1994019463A2 (en) * 1993-02-16 1994-09-01 Auckland Uniservices Limited Developmental tyrosine kinases and their ligands
DE69426076T3 (en) * 1993-05-19 2007-01-18 Schering Corp. PURIFIED FLT3 LIGANDS OF SAEUGENTIANS, AGONISTS AND ANTAGONISTS THEREOF
US7534867B1 (en) 1993-05-19 2009-05-19 Schering Corporation Purified mammalian Flt3 ligands; agonists; antagonists
CZ307995A3 (en) * 1993-05-24 1996-10-16 Immunex Corp Ligands for flt3 receptors
US6630143B1 (en) * 1993-05-24 2003-10-07 Immunex Corporation Antibodies against flt3 ligand
US7045128B2 (en) 1993-05-24 2006-05-16 Immunex Corporation Antibodies against flt3-ligand
US5554512A (en) * 1993-05-24 1996-09-10 Immunex Corporation Ligands for flt3 receptors
AU7210394A (en) * 1993-06-18 1995-01-17 Trustees Of Princeton University, The Totipotent hematopoietic stem cell receptors and their ligands
US5516658A (en) * 1993-08-20 1996-05-14 Immunex Corporation DNA encoding cytokines that bind the cell surface receptor hek
DE69430313T2 (en) * 1993-09-08 2002-08-08 Imclone Systems Inc MONOCLONAL ANTIBODIES THAT DETECT FLK-2 RECEPTORS AND THE ISOLATION OF PRIMITIVE HEMATOPOIETIC STEM CELL POPULATIONS
AU697142B2 (en) 1993-11-23 1998-10-01 Genentech Inc. Protein tyrosine kinases named Rse
US6001621A (en) * 1993-11-23 1999-12-14 Genetech, Inc. Protein tyrosine kinases
US6190655B1 (en) 1993-12-03 2001-02-20 Immunex Corporation Methods of using Flt-3 ligand for exogenous gene transfer
US5618709A (en) * 1994-01-14 1997-04-08 University Of Pennsylvania Antisense oligonucleotides specific for STK-1 and method for inhibiting expression of the STK-1 protein
US5573935A (en) * 1994-01-18 1996-11-12 The United States Of America As Represented By The Department Of Health And Human Services Protein tyrosine kinase A6
US20030108545A1 (en) * 1994-02-10 2003-06-12 Patricia Rockwell Combination methods of inhibiting tumor growth with a vascular endothelial growth factor receptor antagonist
US6448077B1 (en) * 1994-02-10 2002-09-10 Imclone Systems, Inc. Chimeric and humanized monoclonal antibodies specific to VEGF receptors
US5861499A (en) 1994-02-10 1999-01-19 Imclone Systems Incorporated Nucleic acid molecules encoding the variable or hypervariable region of a monoclonal antibody that binds to an extracellular domain
US6811779B2 (en) * 1994-02-10 2004-11-02 Imclone Systems Incorporated Methods for reducing tumor growth with VEGF receptor antibody combined with radiation and chemotherapy
US5840301A (en) * 1994-02-10 1998-11-24 Imclone Systems Incorporated Methods of use of chimerized, humanized, and single chain antibodies specific to VEGF receptors
US7294331B2 (en) * 1994-03-07 2007-11-13 Immunex Corporation Methods of using flt3-ligand in hematopoietic cell transplantation
US7109308B1 (en) 1994-03-08 2006-09-19 Human Genome Sciences, Inc. Antibodies to human vascular endothelial growth factor 2
US6734285B2 (en) * 1994-03-08 2004-05-11 Human Genome Sciences, Inc. Vascular endothelial growth factor 2 proteins and compositions
US5932540A (en) 1994-03-08 1999-08-03 Human Genome Sciences, Inc. Vascular endothelial growth factor 2
US7153827B1 (en) 1994-03-08 2006-12-26 Human Genome Sciences, Inc. Vascular endothelial growth factor 2 and methods of use
US7186688B1 (en) 1994-03-08 2007-03-06 Human Genome Sciences, Inc. Methods of stimulating angiogenesis in a patient by administering vascular endothelial growth factor 2
US6608182B1 (en) 1994-03-08 2003-08-19 Human Genome Sciences, Inc. Human vascular endothelial growth factor 2
ES2249762T3 (en) * 1994-03-08 2006-04-01 Human Genome Sciences, Inc. GROWTH FACTOR OF THE VASCULAR ENDOTELIUM 2.
US6040157A (en) * 1994-03-08 2000-03-21 Human Genome Sciences, Inc. Vascular endothelial growth factor 2
US6140119A (en) * 1994-09-16 2000-10-31 Michigan State University Expression of estrogen receptors in type I and type II human breast epithelial cells
US5650317A (en) * 1994-09-16 1997-07-22 Michigan State University Human breast epithelial cell type with stem cell and luminal epithelial cell characteristics
US5731425A (en) * 1994-10-28 1998-03-24 Eastman Kodak Company Polypeptide surface marker for cells
NZ300808A (en) * 1994-12-23 1999-04-29 Ludwig Inst Cancer Res A method of detecting a ligand capable of binding the receptor protein nyk
US7070972B1 (en) 1995-01-13 2006-07-04 The United States Of America As Represented By The Department Of Health And Human Services Janus family kinases and identification of immune modulators
IL117645A (en) * 1995-03-30 2005-08-31 Genentech Inc Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration
US5824789A (en) * 1995-06-07 1998-10-20 Systemix, Inc. Human growth factors, nucleotide sequence encoding growth factors, and method of use thereof
AU710696C (en) * 1995-09-08 2002-10-24 Genentech Inc. VEGF-related protein
US7150992B1 (en) 1995-10-04 2006-12-19 Innunex Corporation Methods of preparing dendritic cells with flt3-ligand and antigen
US7361330B2 (en) * 1995-10-04 2008-04-22 Immunex Corporation Methods of using flt3-ligand in the treatment of fibrosarcoma
US20020034517A1 (en) * 1995-10-04 2002-03-21 Kenneth Brasel Dendritic cell stimulatory factor
WO1997017442A1 (en) * 1995-11-08 1997-05-15 Immunex Corporation Flk-1 binding protein
US6994989B1 (en) 1995-11-08 2006-02-07 Immunex Corp. FLK-1 binding proteins
US5869243A (en) * 1996-03-05 1999-02-09 Rhode Island Hospital Immortalized hepatocytes
US6100071A (en) 1996-05-07 2000-08-08 Genentech, Inc. Receptors as novel inhibitors of vascular endothelial growth factor activity and processes for their production
US6750044B1 (en) 1996-10-17 2004-06-15 Genentech, Inc. Variants of vascular endothelial cell growth factor having antagonistic properties, nucleic acids encoding the same and host cells comprising those nucleic acids
US20060171907A1 (en) * 1996-11-21 2006-08-03 The Procter & Gamble Company Oral care compositions providing enhanced whitening and stain prevention
AU2469299A (en) * 1998-01-23 1999-08-09 Cornell Research Foundation Inc. Purified populations of stem cells
US6908898B1 (en) * 1998-06-15 2005-06-21 Lars Holmgren Angiogenesis related molecules
WO2000021560A1 (en) * 1998-10-09 2000-04-20 Ludwig Institute For Cancer Research Flt4 (VEGFR-3) AS A TARGET FOR TUMOR IMAGING AND ANTI-TUMOR THERAPY
US8124630B2 (en) * 1999-01-13 2012-02-28 Bayer Healthcare Llc ω-carboxyaryl substituted diphenyl ureas as raf kinase inhibitors
EP2298311B1 (en) * 1999-01-13 2012-05-09 Bayer HealthCare LLC w-Carboxy aryl substituted diphenyl ureas as p38 kinase inhibitors
US20020064528A1 (en) * 2000-01-28 2002-05-30 Zhenping Zhu Antibodies specific to KDR and uses thereof
US7223724B1 (en) * 1999-02-08 2007-05-29 Human Genome Sciences, Inc. Use of vascular endothelial growth factor to treat photoreceptor cells
US6379903B1 (en) 1999-10-08 2002-04-30 Sigma-Aldrich Co. Purification of recombinant proteins fused to multiple epitopes
BR0111530A (en) 2000-06-07 2003-07-22 Ortho Mcneil Pharm Inc Process for detecting vegf kinase domain modulation
NZ518077A (en) * 2000-08-04 2003-11-28 Human Genome Sciences Inc Biologically active fragments, analogues and derivatives of VEGF-2 for the treatment of peripheral artery diseases such as critical limb ischemia and coronary disease
ATE359300T1 (en) 2001-01-19 2007-05-15 Ludwig Inst Cancer Res FLT4 (VEGFR-3) AS A TARGET FOR CANCER DETECTION AND ANTI-CANCER TREATMENT
US7402312B2 (en) * 2001-04-13 2008-07-22 Human Genome Sciences, Inc. Antibodies to vascular endothelial growth factor 2 (VEGF-2)
DE60236646D1 (en) * 2001-04-13 2010-07-22 Human Genome Sciences Inc Anti-VEGF-2 antibodies
US20050232921A1 (en) * 2001-04-13 2005-10-20 Rosen Craig A Vascular endothelial growth factor 2
US20030176674A1 (en) * 2001-04-13 2003-09-18 Rosen Craig A. Vascular endothelial growth factor 2
US20030170786A1 (en) * 2001-04-13 2003-09-11 Rosen Craig A. Vascular endothelial growth factor 2
US7371763B2 (en) * 2001-04-20 2008-05-13 Bayer Pharmaceuticals Corporation Inhibition of raf kinase using quinolyl, isoquinolyl or pyridyl ureas
JP2005508298A (en) * 2001-06-20 2005-03-31 イムクローン システムズ インコーポレイティド Methods for treating atherosclerosis and other inflammatory diseases
JP2005526482A (en) * 2001-08-10 2005-09-08 イムクローン システムズ インコーポレイティド Isolation and mobilization of stem cells expressing VEGFR-1
US20080108672A1 (en) * 2002-01-11 2008-05-08 Bernd Riedl Omega-Carboxyaryl Substituted Diphenyl Ureas As Raf Kinase Inhibitors
MXPA04007832A (en) * 2002-02-11 2005-09-08 Bayer Pharmaceuticals Corp Aryl ureas with angiogenesis inhibiting activity.
US7557129B2 (en) 2003-02-28 2009-07-07 Bayer Healthcare Llc Cyanopyridine derivatives useful in the treatment of cancer and other disorders
CA2526617C (en) * 2003-05-20 2015-04-28 Bayer Pharmaceuticals Corporation Diaryl ureas with kinase inhibiting activity
UA84156C2 (en) 2003-07-23 2008-09-25 Байер Фармасьютикалс Корпорейшн Fluoro substituted omega-carboxyaryl diphenyl urea for the treatment and prevention of diseases and conditions
ES2523457T3 (en) 2004-11-18 2014-11-26 Imclone Llc Antibodies against vascular endothelial growth factor receptor 1
US7495383B2 (en) * 2005-08-01 2009-02-24 Avago Technologies Ecbu Ip (Singapore) Pte. Ltd. Phosphor based on a combination of quantum dot and conventional phosphors
KR100883430B1 (en) * 2007-06-13 2009-02-12 한국생명공학연구원 Human Monoclonal Antibody Neutralizing Vesicular Endothelial Growth Factor Receptor and Use Thereof
AR071891A1 (en) 2008-05-30 2010-07-21 Imclone Llc ANTI-FLT3 HUMAN ANTIBODIES (THIROSINE KINASE 3 RECEPTOR HUMAN FMS TYPE)
CN102171332A (en) * 2008-06-18 2011-08-31 创新生物制剂疗法公司 Methods for enhanced propagation of cells
US9745558B2 (en) 2013-02-18 2017-08-29 Vegenics Pty Limited VEGFR-3 ligand binding molecules and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2035317T5 (en) * 1987-11-09 1998-03-16 Becton Dickinson Co METHOD FOR ANALYZING HEMATOPOYETIC CELLS IN A SAMPLE.
WO1989008114A1 (en) * 1988-02-25 1989-09-08 The General Hospital Corporation Rapid immunoselection cloning method
US5081030A (en) * 1989-04-25 1992-01-14 The Johns Hopkins University Release of cells from affinity matrices
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
US5185438A (en) * 1991-04-02 1993-02-09 The Trustees Of Princeton University Nucleic acids encoding hencatoporetic stem cell receptor flk-2

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
K. M. ZSEBO ET AL.: "Stem cell factor is encoded at the SI locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor.", CELL, vol. 63, 5 October 1990 (1990-10-05), pages 213 - 224, XP000615515 *
K. NOCKA ET AL.: "Candidate ligand for the c-kit transmembrane kinase receptor: KL, a fibroblast derived growth factor stimulates mast cells and erythroid progenitors.", THE EMBO JOURNAL, vol. 9, no. 10, 1990, pages 3287 - 3294, XP000615512 *
K. NOCKA ET AL.: "Expression of c-kit gene products in known cellular targets of W mutations in normal and W mutant mice-evidence for an impaired c-kit kinase in mutant mice.", GENES AND DEVELOPMENT, vol. 3, no. 6, June 1989 (1989-06-01), pages 739 - 920, XP000613737 *

Also Published As

Publication number Publication date
EP1231265A3 (en) 2003-12-03
DE69233788D1 (en) 2010-08-05
US5283354A (en) 1994-02-01
EP0580760B1 (en) 2003-03-12
EP0580760A4 (en) 1997-04-09
EP1231265A2 (en) 2002-08-14
US5621090A (en) 1997-04-15
CA2107463A1 (en) 1992-10-03
DE69232687T2 (en) 2002-11-21
DE69232952D1 (en) 2003-04-17
KR100261465B1 (en) 2000-07-01
EP0909813B1 (en) 2002-07-17
EP1808445A3 (en) 2007-09-26
EP0580760A1 (en) 1994-02-02
EP1231261A3 (en) 2003-12-03
DE69233676D1 (en) 2007-03-22
DE69233676T2 (en) 2007-11-15
WO1992017486A1 (en) 1992-10-15
WO1993000349A1 (en) 1993-01-07
EP1231265B1 (en) 2007-01-31
EP0909813A1 (en) 1999-04-21
AU1924892A (en) 1992-11-02
US5185438A (en) 1993-02-09
EP1231261A2 (en) 2002-08-14
EP1231260B1 (en) 2008-09-17
DE69232952T2 (en) 2003-10-02
EP1808445A2 (en) 2007-07-18
DE69232687D1 (en) 2002-08-22
EP1231260A2 (en) 2002-08-14
EP1231261B1 (en) 2010-06-23
KR100406832B1 (en) 2003-11-21
DE69233746D1 (en) 2008-10-30
KR100407010B1 (en) 2003-11-28
CA2107463C (en) 2008-06-17

Similar Documents

Publication Publication Date Title
EP1231260A3 (en) A method for isolating cells that express FLK-2 receptors on their surface
EP0363126A3 (en) Method for the purification of vitamin K-dependent proteins
WO2003060090A3 (en) Novel cytokine zcytor17 ligand
EP0669929A4 (en) Novel cytokine designated elk ligand.
PL311142A1 (en) Method of and product for controlling cell ability to respond to external signals
WO1991008298A3 (en) Fusion proteins consisting of a ligand binding protein and a stable plasma protein
IL109677A (en) Ligands for flt3 receptors, their production and pharmaceutical compositions containing them
WO2000001823A3 (en) Flt3-l mutants and methods of use
AU2212088A (en) Method for culturing recombinant cells
AU3759693A (en) Peptide library and screening method
IL130403A (en) Oil bodies and associated proteins as affinity matrices
Yamagami Isolation of a choriolytic enzyme (hatching enzyme) of the teleost, Oryzias latipes
DE69738432D1 (en) TARGETING OF ADENOVIRES WITH THE HELP OF CONFORT-RESTRICTED PEPTIDE MOTIVES
HUP0000317A3 (en) Process for the preparation of recombinant proteins in e.coli by high cell density fermentation
AU3801997A (en) Assays for protein kinases using fluorescent protein substrates
WO1995000554A3 (en) Totipotent hematopoietic stem cell receptors and their ligands
WO1998040498A3 (en) Immunoadhesins and methods of production and use thereof
PL287102A1 (en) Agent for chromatographic separation containing a ligand, method for manufactoring the agent and a method for appylying the agent to separate synthetic or natural particles from a liquid mixture
WO1997022690A3 (en) SIGNALING INOSITOL POLYPHOSPHATE 5-PHOSPHATASES (SIPs)
Köck et al. Purification of human interleukin 1 by high-performance liquid chromatography
IL127363A0 (en) IL-1/TNF-alpha-activated kinase and methods of making and using the same
WO1994019463A3 (en) Developmental tyrosine kinases and their ligands
Varon et al. The nerve growth factor proteins of the mouse submaxillary gland
Bibi Purification of TEM-1 beta-lactamase by immunoaffinity chromatography.
Hah et al. Changes in Pectoral Myoblast Proteins during Myofibrillogenesis

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20020222

AC Divisional application: reference to earlier application

Ref document number: 580760

Country of ref document: EP

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): DE FR GB IT

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): DE FR GB IT

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 07K 16/28 B

Ipc: 7C 07K 14/715 A

Ipc: 7C 12N 5/06 B

Ipc: 7C 07K 14/52 B

AKX Designation fees paid

Designated state(s): DE FR GB IT

17Q First examination report despatched

Effective date: 20060314

17Q First examination report despatched

Effective date: 20060314

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AC Divisional application: reference to earlier application

Ref document number: 0580760

Country of ref document: EP

Kind code of ref document: P

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): DE FR GB IT

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REF Corresponds to:

Ref document number: 69233746

Country of ref document: DE

Date of ref document: 20081030

Kind code of ref document: P

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20090618

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20110331

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20110429

Year of fee payment: 20

Ref country code: GB

Payment date: 20110328

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20110423

Year of fee payment: 20

REG Reference to a national code

Ref country code: DE

Ref legal event code: R071

Ref document number: 69233746

Country of ref document: DE

REG Reference to a national code

Ref country code: DE

Ref legal event code: R071

Ref document number: 69233746

Country of ref document: DE

REG Reference to a national code

Ref country code: GB

Ref legal event code: PE20

Expiry date: 20120401

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20120401

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DE

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20120403