US20020159921A1 - Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor - Google Patents
Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor Download PDFInfo
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- US20020159921A1 US20020159921A1 US10/178,271 US17827102A US2002159921A1 US 20020159921 A1 US20020159921 A1 US 20020159921A1 US 17827102 A US17827102 A US 17827102A US 2002159921 A1 US2002159921 A1 US 2002159921A1
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- Prior art keywords
- droplet
- transfer tube
- displacement
- displacement gas
- liquid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
- Y10T436/119163—Automated chemical analysis with aspirator of claimed structure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Definitions
- the invention relates to a method for depositing a flattened droplet on a surface suitable for in vitro fertilization (IVF) and embryo transfer (ET), and apparatus therefor, and a pump therefor.
- IVF in vitro fertilization
- ET embryo transfer
- oocytes are aspirated from female ovaries and inseminated in vitro with male sperm in a culture medium to form embryos which are incubated prior to their being transferred to a subject by means of a two-stage transfer procedure.
- the transfer procedure includes the aspiration of a relatively large volume of about 20-40 ⁇ l of culture medium containing between one to five embryos into a transfer catheter by means of a manually operated syringe and its subsequent injection into a subject.
- the present invention is based on the notion that substantially automating embryo implantation in IVF-ET procedures may overcome some of the shortcomings of the conventional IVF-ET procedure.
- a method for depositing a flattened droplet on a partially absorbent surface comprising the steps of:
- a “flattened droplet” in the context of the present invention can be demonstrated on standard 80 gram/m 2 A4 paper for use with ink jet printers, such paper constituting a partially absorbent surface on which a flattened droplet of the present invention has a projected surface area about three to six times larger than that of a naturally forming dome-like droplet.
- a “partially absorbent surface” in the context of the present invention is one which absorbs a relatively insignificant volume of a naturally forming dome-like droplet over about 60 seconds.
- the flattening of a droplet as achieved by the method of the present invention is not by the relatively slow process of its being absorbed assuming it does not dry but rather as a consequence of its being effectively inflated by one or more bubbles of displacement gas controllably blown thereinto towards the end of its discharge which typically occurs over 5-20 seconds from an initial outward displacement of the microvolume of liquid.
- the surface may be flat, inclined or even inverted and still maintain the droplet in its flattened shape by virtue of the prevailing surface tension therewith.
- a “microvolume of liquid” in the context of the present invention is a volume of liquid in the microliter range, e.g., within the range of 0.05-5 ⁇ l, preferably within the range of 0.1-3.0 ⁇ l, and particularly within the range of 0.3-2.0 ⁇ l.
- a volume of liquid in the microliter range e.g., within the range of 0.05-5 ⁇ l, preferably within the range of 0.1-3.0 ⁇ l, and particularly within the range of 0.3-2.0 ⁇ l.
- step (a) includes:
- the step of preventing capillary forces is preferably achieved by issuing an outgoing flow of displacement gas into the transfer tube so as to create a positive pressure therein.
- this step can be achieved, for example, by providing a seal at its distal end.
- the microvolume of liquid is preferably inwardly drawn away from its distal end as a safety precaution whereafter the inward displacement is neutralized by a brief outgoing flow of displacement gas into the transfer tube.
- apparatus for depositing a flattened droplet of liquid on a partially absorbent surface the apparatus for use with a narrow bore transfer tube having a proximal end and a distal end and a vessel of liquid, the apparatus comprising:
- a user controlled suction mode for drawing an incoming flow of displacement gas from the transfer tube whereby a microvolume of liquid is drawn thereinto prior to the removal of said distal end from the vessel;
- a user initiated automated delivery mode for issuing an outgoing flow of displacement gas into the transfer tube for slowly discharging substantially the entire microvolume of liquid as a droplet on the surface and controllably blowing one or more bubbles into the droplet towards the end of its discharge to flatten the droplet on the surface.
- a pump comprising a housing having a bore with an internal peripheral surface, and a stationary annular sealing member integrally formed therewith at a first end of a pair of opposite ends; a slide rod disposed in said bore and having an external peripheral surface and a displaceable annular sealing member integrally formed therewith; said sealing members sealing said peripheral surfaces to define a vented displacement volume therebetween whose volume is proportional to an annular cross section area between said peripheral surfaces and a distance between said sealing members; and said slide rod being slidably reciprocable between first and second positions respectively toward and away from said stationary sealing member whereupon said displaceable sealing member moves to reduce said volume to issue an outgoing flow of displacement gas from said displacement volume on a downstroke of said slide rod from said second position to said first position and said displaceable sealing member moves to increase said volume to draw an incoming flow of displacement gas into said displacement volume on an upstroke of said slide rod from said first position to said second position.
- both the bore and the slide rod are of a right cylindrical shape such that the displacement volume has a cross sectional area defined by ⁇ (a 2 -b 2 ) where a and b are the radii of the bore's internal peripheral surface and the slide rod's external peripheral surface, respectively.
- the cross section area is in the order of 4-10 mm 2 and the pump has a displacement volume incrementally changeable in the order of 0.1-0.4 ⁇ l.
- FIG. 1 is a pictorial view of apparatus for depositing a flattened droplet on a surface in accordance with the present invention
- FIGS. 2 A- 2 L illustrates operation of the apparatus of FIG. 1 for depositing the flattened droplet on the surface
- FIG. 3 is a pictorial view of a pump in accordance with the present invention.
- FIGS. 4 and 5 are cross sectional views of the pump of FIG. 3 along lines A-A and B-B in FIG. 3, respectively.
- apparatus 1 is employed for depositing a flattened droplet F on a partially absorbent surface S, for example, on a subject's endometrium in an IVF-ET procedure.
- Apparatus 1 includes a suction control unit 2 normally permanent located in a laboratory for the preparation of an embryo carrying catheter 3 constituting a transfer tube, a transfer control unit 4 normally permanently located in a treatment room where an IVF-ET procedure is carried out and a portable casing 6 for consecutive connection to the suction control unit 2 and the transfer control unit 4 by means of connectors 7 and 8 .
- the casing 6 includes a pneumatic system 9 which is permanently connected to the catheter 3 during an entire IVF-ET procedure via suitable air tubing 11 and an air filter 12 .
- the casing 6 also has a receptacle 13 for accommodating the catheter 3 during its transport from the laboratory to the treatment room.
- the pneumatic system 9 is under a control mechanism 14 including a computer mouse 16 for controlling the suction control unit 2 for initiating a user controlled suction mode to prepare the catheter 3 with a microvolume of embryo containing culture medium and a foot pedal 17 for controlling the transfer control unit 4 for initiating a user initiated automated delivery mode for depositing the flattened droplet F on the surface S.
- the computer mouse 16 has an upstroke control 18 for drawing an incoming flow of displacement gas into the pneumatic system 9 from the catheter 3 , a downstroke control 19 for issuing an outgoing flow of displacement gas from the pneumatic system 9 into the catheter 3 and optionally a speed control 21 for controlling the flow rate of the displacement gas either from or into the pneumatic system 9 .
- the suction control unit 2 is also provided with a reset button 22 for priming the pneumatic system 9 for a pre-suction mode of issuing an outgoing flow of displacement gas as indicated by a READY indicator light 23 prior to the preparation of the catheter 3 .
- the different stages of the automatic delivery mode are indicated by a READY indicator light 24 , a GO indicator light 26 and a DONE indicator light 27 .
- the casing 6 is initially connected to the suction control unit 2 and the catheter 3 is connected to the pneumatic system 9 via the air tubing 11 and the filter 12 .
- An operator presses the reset button 22 whereupon a lit READY indicator light 23 indicates an outgoing flow of displacement gas creating a positive pressure within catheter 3 to prevent capillary forces drawing culture medium thereinto upon insertion of its distal end 3 A into a vessel of culture medium C containing embryos E shown exaggerated in all FIGS. 2 A- 2 L (see FIG. 2A).
- the operator inserts the distal end 3 A into the vessel of culture medium C for aspirating about 0.3 to 0.6 ⁇ l microvolume of culture medium containing an embryo E into the catheter 3 (see FIG. 2B).
- the rate of aspiration of culture medium may be increased by depressing the speed control 21 . If a single embryo is to be transferred, distal end 3 A is then be removed from the culture medium otherwise additional embryos may be captured as shown.
- the operator withdraws its distal end 3 A from the culture medium and then proceeds to depress the downstroke control 19 to slowly displace the microvolume of culture medium inwardly (see FIG. 2C).
- the microvolume of culture medium has been inwardly displaced by about 5-15 mm from the catheter's distal end 3 A, its motion is arrested by a momentary outgoing flow of displacement gas (see FIG. 2D) so that it finally comes to rest at a distance of about 10 mm (see FIG. 2E) therefrom thereby ensuring that it cannot be inadvertently lost during transportation of the casing 6 between the laboratory and the treatment room.
- the catheter 3 is then placed in the receptacle 13 (see FIG. 1) during the transportation of the casing 6 from the laboratory to the treatment room.
- the catheter 3 is laid on the surface S (see FIG. 2F) whereupon a first depression on the foot pedal 17 causes the READY indicator light 24 to be lit indicating that the automatic delivery mode can be initiated. Thereafter, a second depression on the foot pedal 17 causes the GO indicator light 26 to be lit indicating that an outgoing flow of displacement gas is displacing the microvolume of culture medium towards the catheter's distal end 3 A (see FIG. 2G).
- the outgoing flow of displacement gas causes a concave shaped meniscus to be slowly formed which increases in size until it suddenly ruptures whereby most of the microvolume of culture medium is discharged as a droplet D on the surface S (see FIGS. 2H and 2J).
- the discharge is accompanied by one or more air bubbles B for effectively inflating the droplet D thereby considerably widening its projected surface area on the surface S to form the flattened droplet F whose shape is maintained by its prevailing surface tension with the surface S (see FIG. 2K).
- the GO indicator light 26 is then extinguished indicating that the operator should slightly withdraw the catheter 3 so as to detach it from the droplet F whilst at the same time there is an outgoing flow of displacement gas (see FIG. 2L).
- withdrawal is limited to between about 10-15 mm such that the catheter's distal end 3 A still lies along a subject's endometrium.
- a further outgoing flow of additional displacement gas is provided so as to remove any culture medium which may remain in the catheter 3 .
- the DONE indicator light 27 is then lit to indicate that the catheter 3 can be completely removed.
- a pump 31 constituting a pneumatic system for use with the apparatus 1 includes a base 32 with a housing 33 having a longitudinal right cylindrical throughbore 34 with an internal peripheral surface 36 of a radius a and having first and second opposite ends 37 and 38 .
- a right cylindrical slide rod 39 with an external peripheral surface 41 of a radius b and first and second opposite end 42 and 43 is disposed in the bore 34 and is slidingly reciprocated by means of a linear actuator screw 44 driven by a step motor 46 .
- the displacement volume 51 has a volume equal to a product of a cross sectional area between the surface 36 and 41 defined by ⁇ (a 2 -b 2 ) and the distance between the gaskets 48 and 49 .
- the slide rod 39 is slidingly reciprocable between first and second positions respectively toward and away from the gasket 48 whereupon the displacement volume 51 has a minimum value when the gaskets 48 and 49 are adjacent in which case a major portion of the slide rod 39 is exterior to the bore 34 and a maximum value when the gaskets 48 and 49 are remote from one another.
- the gasket 49 moves to reduce the volume of the displacement volume 51 to issue an outgoing flow of displacement gas therefrom on a downstroke of the slide rod 39 from its second position to its first position and the gasket 49 moves to increase the volume of the displacement volume 51 to draw an incoming flow of displacement gas thereinto on an upstroke of the slide rod 39 from its first position to its second position.
- the bore 34 and the slide rod 39 typically have diameters in the range of about 5-10 mm and which differ in the range of about 0.3-0.7 mm such that the cross section area is in the order of about 4-10 mm 2 .
- the threading on actuator screw 44 is designed such that each step of the motor 45 causes an incremental movement of the slide rod 39 of about 0.001-0.002 inches.
- the motor 45 is typically driven at a rate of about 20-300 steps per second.
Abstract
A method and apparatus therefor for depositing a flattened droplet on a partially absorbent surface comprising the steps of providing a narrow bore transfer tube having a proximal end and a distal end and containing a microvolume of liquid, the proximal end connected to a pneumatic system adapted for issuing an outgoing flow of displacement gas into the tube and drawing an incoming flow of displacement gas therefrom; and issuing an outgoing flow of displacement gas for slowly discharging substantially the entire microvolume of liquid as a droplet on the surface and controllably blowing one or more bubbles into the droplet towards the end of its discharge for flattening the droplet on the surface. A pump (31) comprising a housing (33) having a bore (34) with an internal peripheral surface (36), and a stationary annular sealing member (48) integrally formed therewith at a first end (37) of a pair of opposite ends (37 and 38); a slide rod (39) disposed in the bore and having an external peripheral surface (41) and a displaceable annular sealing member (49) integrally formed therewith; the sealing members sealing the peripheral surfaces to define a vented displacement volume (51) therebetween whose volume is proportional to an annular cross section area between the peripheral surfaces and a distance between the sealing members; and the slide rod being slidably reciprocable between first and second positions respectively toward and away from the stationary sealing member whereupon the displaceable sealing member moves to reduce the volume to issue an outgoing flow of displacement gas from the displacement volume on a downstroke of the slide rod from its second position to its first position and the displaceable sealing member moves to increase the volume to draw an incoming flow of displacement gas into the displacement volume on an upstroke of the slide rod from its first position to its position.
Description
- The invention relates to a method for depositing a flattened droplet on a surface suitable for in vitro fertilization (IVF) and embryo transfer (ET), and apparatus therefor, and a pump therefor.
- In an IVF-ET procedure, oocytes are aspirated from female ovaries and inseminated in vitro with male sperm in a culture medium to form embryos which are incubated prior to their being transferred to a subject by means of a two-stage transfer procedure. The transfer procedure includes the aspiration of a relatively large volume of about 20-40 μl of culture medium containing between one to five embryos into a transfer catheter by means of a manually operated syringe and its subsequent injection into a subject.
- Since a conventional IVF-ET procedure is manual, the rate of injection of the embryo containing culture into a uterine cavity may vary significantly. Thus, on the one hand, too slow an injection rate may cause embryo containing culture medium to trickle down the transfer catheter's outer surface and, on the other hand, too quick an injection rate may severely damage embryo(s) following their collision against a uterine wall. The latter case may also flood a subject's uterus possibly resulting in a failed procedure as embryos are either washed out of her uterus or implanted in one of her Fallopian tubes leading to an ectopic pregnancy. Another disadvantage attendant with the use of a relatively large volume of culture medium is that it may alter the specific properties of the micro-environment within a subject's uterus required for successful embryo implantation and its normal development.
- The present invention is based on the notion that substantially automating embryo implantation in IVF-ET procedures may overcome some of the shortcomings of the conventional IVF-ET procedure.
- In accordance with the present invention, there is provided a method for depositing a flattened droplet on a surface particularly suitable for depositing embryo(s) containing culture medium at a desired site in a uterus cavity, and apparatus therefor, and a pump therefor.
- In accordance with a first aspect of the invention, there is provided a method for depositing a flattened droplet on a partially absorbent surface, the method comprising the steps of:
- (a) providing a narrow bore transfer tube having a proximal end and a distal end and containing a microvolume of liquid, the proximal end connected to a pneumatic system for issuing an outgoing flow of displacement gas into the transfer tube and drawing an incoming flow of displacement gas thereinto from the transfer tube; and
- (b) issuing an outgoing flow of displacement gas for slowly discharging substantially the entire microvolume of liquid as a droplet on the surface and controllably blowing one or more bubbles into the droplet towards the end of its discharge to flatten the droplet on the surface.
- A “flattened droplet” in the context of the present invention can be demonstrated on standard 80 gram/m2 A4 paper for use with ink jet printers, such paper constituting a partially absorbent surface on which a flattened droplet of the present invention has a projected surface area about three to six times larger than that of a naturally forming dome-like droplet. A “partially absorbent surface” in the context of the present invention is one which absorbs a relatively insignificant volume of a naturally forming dome-like droplet over about 60 seconds. The flattening of a droplet as achieved by the method of the present invention is not by the relatively slow process of its being absorbed assuming it does not dry but rather as a consequence of its being effectively inflated by one or more bubbles of displacement gas controllably blown thereinto towards the end of its discharge which typically occurs over 5-20 seconds from an initial outward displacement of the microvolume of liquid. The surface may be flat, inclined or even inverted and still maintain the droplet in its flattened shape by virtue of the prevailing surface tension therewith.
- A “microvolume of liquid” in the context of the present invention is a volume of liquid in the microliter range, e.g., within the range of 0.05-5 μl, preferably within the range of 0.1-3.0 μl, and particularly within the range of 0.3-2.0 μl. In the case of an IVF-ET procedure on a human subject when the catheter is upwardly inclined, even though the discharge of culture medium is relatively slow, its volume is so small so as to avoid a downward trickle along the catheter's outer surface.
- In accordance with a preferred embodiment of the present invention, step (a) includes:
- (a1) preventing capillary forces to draw liquid into the transfer tube upon insertion of its distal end into a vessel containing liquid;
- (a2) inserting the transfer tube's distal end into the liquid;
- (a3) drawing an incoming flow of displacement gas from the transfer tube such that a microvolume of liquid is drawn thereinto; and
- (a4) removing the transfer tube's distal end from the liquid.
- The step of preventing capillary forces is preferably achieved by issuing an outgoing flow of displacement gas into the transfer tube so as to create a positive pressure therein. Alternatively, this step can be achieved, for example, by providing a seal at its distal end. After the transfer tube's distal end is removed from the liquid, the microvolume of liquid is preferably inwardly drawn away from its distal end as a safety precaution whereafter the inward displacement is neutralized by a brief outgoing flow of displacement gas into the transfer tube.
- After the discharge of the microvolume of liquid from the transfer tube, the flattened droplet in most cases is still connected to the transfer tube's distal end and therefore to prevent its suction back into the transfer tube, the outgoing flow of displacement gas is maintained until the droplet is disconnected from the transfer tube's distal end by manually withdrawing the transfer tube. This outgoing flow of displacement gas may also remove any small quantities of the microvolume which were not initially discharged and which may include an embryo(s) in an IVF-ET procedure.
- In accordance with a second aspect of the present invention, there is provided apparatus for depositing a flattened droplet of liquid on a partially absorbent surface, the apparatus for use with a narrow bore transfer tube having a proximal end and a distal end and a vessel of liquid, the apparatus comprising:
- (a) a pneumatic system connected to the transfer tube's proximal end and adapted for issuing an outgoing flow of displacement gas into said transfer tube and drawing an incoming flow of displacement gas thereinto from said transfer tube; and
- (b) a control mechanism for controlling said pneumatic system in different operational modes including:
- a user controlled suction mode for drawing an incoming flow of displacement gas from the transfer tube whereby a microvolume of liquid is drawn thereinto prior to the removal of said distal end from the vessel; and
- a user initiated automated delivery mode for issuing an outgoing flow of displacement gas into the transfer tube for slowly discharging substantially the entire microvolume of liquid as a droplet on the surface and controllably blowing one or more bubbles into the droplet towards the end of its discharge to flatten the droplet on the surface.
- In accordance with a third aspect of the present invention, there is provided a pump comprising a housing having a bore with an internal peripheral surface, and a stationary annular sealing member integrally formed therewith at a first end of a pair of opposite ends; a slide rod disposed in said bore and having an external peripheral surface and a displaceable annular sealing member integrally formed therewith; said sealing members sealing said peripheral surfaces to define a vented displacement volume therebetween whose volume is proportional to an annular cross section area between said peripheral surfaces and a distance between said sealing members; and said slide rod being slidably reciprocable between first and second positions respectively toward and away from said stationary sealing member whereupon said displaceable sealing member moves to reduce said volume to issue an outgoing flow of displacement gas from said displacement volume on a downstroke of said slide rod from said second position to said first position and said displaceable sealing member moves to increase said volume to draw an incoming flow of displacement gas into said displacement volume on an upstroke of said slide rod from said first position to said second position.
- Preferably both the bore and the slide rod are of a right cylindrical shape such that the displacement volume has a cross sectional area defined by π(a2-b2) where a and b are the radii of the bore's internal peripheral surface and the slide rod's external peripheral surface, respectively. Preferably, the cross section area is in the order of 4-10 mm2 and the pump has a displacement volume incrementally changeable in the order of 0.1-0.4 μl.
- In order to understand the invention and how it is used, a preferred embodiment will now be described by way of a non-limiting example only, with reference to the accompanying drawings in which:
- FIG. 1 is a pictorial view of apparatus for depositing a flattened droplet on a surface in accordance with the present invention;
- FIGS.2A-2L illustrates operation of the apparatus of FIG. 1 for depositing the flattened droplet on the surface;
- FIG. 3 is a pictorial view of a pump in accordance with the present invention; and
- FIGS. 4 and 5 are cross sectional views of the pump of FIG. 3 along lines A-A and B-B in FIG. 3, respectively.
- With reference now to FIGS. 1 and 2, apparatus1 is employed for depositing a flattened droplet F on a partially absorbent surface S, for example, on a subject's endometrium in an IVF-ET procedure. Apparatus 1 includes a
suction control unit 2 normally permanent located in a laboratory for the preparation of anembryo carrying catheter 3 constituting a transfer tube, atransfer control unit 4 normally permanently located in a treatment room where an IVF-ET procedure is carried out and aportable casing 6 for consecutive connection to thesuction control unit 2 and thetransfer control unit 4 by means ofconnectors casing 6 includes apneumatic system 9 which is permanently connected to thecatheter 3 during an entire IVF-ET procedure viasuitable air tubing 11 and anair filter 12. Thecasing 6 also has areceptacle 13 for accommodating thecatheter 3 during its transport from the laboratory to the treatment room. - The
pneumatic system 9 is under acontrol mechanism 14 including acomputer mouse 16 for controlling thesuction control unit 2 for initiating a user controlled suction mode to prepare thecatheter 3 with a microvolume of embryo containing culture medium and afoot pedal 17 for controlling thetransfer control unit 4 for initiating a user initiated automated delivery mode for depositing the flattened droplet F on the surface S. Thecomputer mouse 16 has anupstroke control 18 for drawing an incoming flow of displacement gas into thepneumatic system 9 from thecatheter 3, adownstroke control 19 for issuing an outgoing flow of displacement gas from thepneumatic system 9 into thecatheter 3 and optionally aspeed control 21 for controlling the flow rate of the displacement gas either from or into thepneumatic system 9. Thesuction control unit 2 is also provided with areset button 22 for priming thepneumatic system 9 for a pre-suction mode of issuing an outgoing flow of displacement gas as indicated by aREADY indicator light 23 prior to the preparation of thecatheter 3. The different stages of the automatic delivery mode are indicated by aREADY indicator light 24, aGO indicator light 26 and aDONE indicator light 27. - In operation, the
casing 6 is initially connected to thesuction control unit 2 and thecatheter 3 is connected to thepneumatic system 9 via theair tubing 11 and thefilter 12. An operator presses thereset button 22 whereupon a litREADY indicator light 23 indicates an outgoing flow of displacement gas creating a positive pressure withincatheter 3 to prevent capillary forces drawing culture medium thereinto upon insertion of itsdistal end 3A into a vessel of culture medium C containing embryos E shown exaggerated in all FIGS. 2A-2L (see FIG. 2A). The operator inserts thedistal end 3A into the vessel of culture medium C for aspirating about 0.3 to 0.6 μl microvolume of culture medium containing an embryo E into the catheter 3 (see FIG. 2B). Once an embryo is clearly seen to be close to the catheter'sdistal end 3A, the rate of aspiration of culture medium may be increased by depressing thespeed control 21. If a single embryo is to be transferred,distal end 3A is then be removed from the culture medium otherwise additional embryos may be captured as shown. - Once the
catheter 3 contains one or more embryos, the operator withdraws itsdistal end 3A from the culture medium and then proceeds to depress thedownstroke control 19 to slowly displace the microvolume of culture medium inwardly (see FIG. 2C). After the microvolume of culture medium has been inwardly displaced by about 5-15 mm from the catheter'sdistal end 3A, its motion is arrested by a momentary outgoing flow of displacement gas (see FIG. 2D) so that it finally comes to rest at a distance of about 10 mm (see FIG. 2E) therefrom thereby ensuring that it cannot be inadvertently lost during transportation of thecasing 6 between the laboratory and the treatment room. Thecatheter 3 is then placed in the receptacle 13 (see FIG. 1) during the transportation of thecasing 6 from the laboratory to the treatment room. - For transfer of the embryos E onto the surface S, the
catheter 3 is laid on the surface S (see FIG. 2F) whereupon a first depression on thefoot pedal 17 causes the READY indicator light 24 to be lit indicating that the automatic delivery mode can be initiated. Thereafter, a second depression on thefoot pedal 17 causes the GO indicator light 26 to be lit indicating that an outgoing flow of displacement gas is displacing the microvolume of culture medium towards the catheter'sdistal end 3A (see FIG. 2G). The outgoing flow of displacement gas causes a concave shaped meniscus to be slowly formed which increases in size until it suddenly ruptures whereby most of the microvolume of culture medium is discharged as a droplet D on the surface S (see FIGS. 2H and 2J). The discharge is accompanied by one or more air bubbles B for effectively inflating the droplet D thereby considerably widening its projected surface area on the surface S to form the flattened droplet F whose shape is maintained by its prevailing surface tension with the surface S (see FIG. 2K). - The GO indicator light26 is then extinguished indicating that the operator should slightly withdraw the
catheter 3 so as to detach it from the droplet F whilst at the same time there is an outgoing flow of displacement gas (see FIG. 2L). In the case of an actual IVF-ET procedure, withdrawal is limited to between about 10-15 mm such that the catheter'sdistal end 3A still lies along a subject's endometrium. Finally, a further outgoing flow of additional displacement gas is provided so as to remove any culture medium which may remain in thecatheter 3. The DONE indicator light 27 is then lit to indicate that thecatheter 3 can be completely removed. - With reference now to FIGS.3-5, a
pump 31 constituting a pneumatic system for use with the apparatus 1 includes a base 32 with ahousing 33 having a longitudinal rightcylindrical throughbore 34 with an internalperipheral surface 36 of a radius a and having first and second opposite ends 37 and 38. A rightcylindrical slide rod 39 with an externalperipheral surface 41 of a radius b and first and secondopposite end bore 34 and is slidingly reciprocated by means of alinear actuator screw 44 driven by astep motor 46. - A
sleeve bearing 47 having a sealing O-ring gasket 48 constituting a stationary annular sealing member is disposed at the first end 37 and an O-ring gasket 49 constituting a displaceable annular sealing member is disposed at theslide rod end 42, thegaskets peripheral surfaces displacement volume 51 vented by avent 52. Thedisplacement volume 51 has a volume equal to a product of a cross sectional area between thesurface gaskets - The
slide rod 39 is slidingly reciprocable between first and second positions respectively toward and away from thegasket 48 whereupon thedisplacement volume 51 has a minimum value when thegaskets slide rod 39 is exterior to thebore 34 and a maximum value when thegaskets gasket 49 moves to reduce the volume of thedisplacement volume 51 to issue an outgoing flow of displacement gas therefrom on a downstroke of theslide rod 39 from its second position to its first position and thegasket 49 moves to increase the volume of thedisplacement volume 51 to draw an incoming flow of displacement gas thereinto on an upstroke of theslide rod 39 from its first position to its second position. - The
bore 34 and theslide rod 39 typically have diameters in the range of about 5-10 mm and which differ in the range of about 0.3-0.7 mm such that the cross section area is in the order of about 4-10 mm2. The threading onactuator screw 44 is designed such that each step of the motor 45 causes an incremental movement of theslide rod 39 of about 0.001-0.002 inches. The motor 45 is typically driven at a rate of about 20-300 steps per second. - Various modifications and changes may be made in the configuration described above that come within the spirit of the invention. The invention embraces all such changes and modifications coming within the scope of the appended claims.
Claims (13)
1. A method for depositing a flattened droplet on a partially absorbent surface comprising the steps of:
(a) providing a narrow bore transfer tube having a proximal end and a distal end and containing a microvolume of liquid, the proximal end connected to a pneumatic system adapted for issuing an outgoing flow of displacement gas into the tube and drawing an incoming flow of displacement gas therefrom; and
(b) issuing an outgoing flow of displacement gas for slowly discharging substantially the entire microvolume of liquid as a droplet on the surface and controllably blowing one or more bubbles into the droplet towards the end of its discharge for flattening the droplet on the surface.
2. The method of claim 1 wherein step (a) includes:
(a1) preventing capillary forces to draw liquid into the transfer tube upon the insertion of its distal end into a vessel containing liquid;
(a2) inserting the transfer tube's distal end into the vessel;
(a3) drawing an incoming flow of displacement gas from the transfer tube such that a microvolume of liquid is drawn thereinto; and
(a4) removing the transfer tube's distal end from the liquid.
3. The method of claim 2 wherein step (a1) includes issuing an outgoing flow of displacement gas into the transfer tube.
4. The method of claim 3 further comprising the step of:
(a5) drawing the microvolume of liquid into the transfer tube away from its distal end; and
(a6) neutralizing the inward displacement by a brief outgoing flow of displacement gas into the transfer tube.
5. The method according to any one of claims 1-4 further comprising the step of:
(c) providing additional outflow of displacement gas while displacing the transfer tube away from the droplet so separate the droplet from its distal end.
6. The method according to any one of claims 1-5 wherein the microvolume of liquid is a culture medium containing embryo(s) which are urged against the surface by the droplet's prevailing surface tension.
7. Apparatus for depositing a flattened droplet on a partially absorbent surface, the apparatus for use with a narrow bore transfer tube having a proximal end and a distal end and a vessel of liquid, the apparatus comprising:
(a) a pneumatic system connected to the transfer tube's proximal end and adapted for issuing an outgoing flow of displacement gas into said transfer tube and drawing an incoming flow of displacement gas thereinto from said transfer tube; and
(b) a control mechanism for controlling said pneumatic system in different operational modes including:
an user controlled suction mode for drawing an incoming flow of displacement gas from said transfer tube whereby a microvolume of liquid is drawn thereinto prior to the removal of said distal end from the vessel; and
an user initiated automated delivery mode for issuing an outgoing flow of displacement gas into said transfer tube for slowly discharging substantially the entire microvolume of liquid as a droplet on the surface and controllably blowing one or more bubbles into the droplet towards the end of its discharge for flattening the droplet on the surface.
8. Apparatus according to claim 7 wherein said control mechanism includes an automatic pre-suction mode for issuing an outgoing flow of displacement gas.
9. Apparatus according to claim 7 or 8 wherein separate units house a suction control unit for controlling said user controlled suction mode and a transfer control unit for controlling said user initiated automatic delivery mode.
10. A pump comprising a housing having a bore with an internal peripheral surface, and a stationary annular sealing member integrally formed therewith at a first end of a pair of opposite ends; a slide rod disposed in said bore and having an external peripheral surface and a displaceable annular sealing member integrally formed therewith; said sealing members sealing said peripheral surfaces to define a vented displacement volume therebetween whose volume is proportional to an annular cross section area between said peripheral surfaces and a distance between said sealing members; and said slide rod being slidably reciprocable between first and second positions respectively toward and away from said stationary sealing member whereupon said displaceable sealing member moves to reduce said volume to issue an outgoing flow of displacement gas from said displacement volume on a downstroke of said slide rod from said second position to said first position and said displaceable sealing member moves to increase said volume to draw an incoming flow of displacement gas into said displacement volume on an upstroke of said slide rod from said first position to said second position.
11. The pump according to claim 10 wherein both said bore and said slide rod are of right cylindrical shape and said cross section area is defined by π(a2-b2) where a and b are the radii of said internal peripheral surface and said external peripheral surface, respectively.
12. The pump according to either claim 10 or 11 wherein said cross section area has an area of about 4-10 mm2.
13. The pump according to any one of claims 10-12 wherein said displacement volume can be incrementally changed in the order of 0.1-0.4 μl.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/178,271 US20020159921A1 (en) | 1997-10-14 | 2002-06-25 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL12196897A IL121968A0 (en) | 1997-10-14 | 1997-10-14 | Method for depositing a microvolume of liquid on a surface and apparatus therefor and a microvolume pump therefor |
IL121968 | 1997-10-14 | ||
US09/529,065 US6420186B1 (en) | 1997-10-14 | 1998-10-14 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
PCT/IL1998/000498 WO1999018872A1 (en) | 1997-10-14 | 1998-10-14 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
ILPCT/IL98/00498 | 1998-10-14 | ||
US10/178,271 US20020159921A1 (en) | 1997-10-14 | 2002-06-25 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/529,065 Division US6420186B1 (en) | 1997-10-14 | 1998-10-14 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
Publications (1)
Publication Number | Publication Date |
---|---|
US20020159921A1 true US20020159921A1 (en) | 2002-10-31 |
Family
ID=11070748
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/529,065 Expired - Fee Related US6420186B1 (en) | 1997-10-14 | 1998-10-14 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
US10/178,271 Abandoned US20020159921A1 (en) | 1997-10-14 | 2002-06-25 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/529,065 Expired - Fee Related US6420186B1 (en) | 1997-10-14 | 1998-10-14 | Method for depositing a flattened droplet on a surface and apparatus therefor, and a pump therefor |
Country Status (4)
Country | Link |
---|---|
US (2) | US6420186B1 (en) |
EP (1) | EP1121060A1 (en) |
IL (1) | IL121968A0 (en) |
WO (1) | WO1999018872A1 (en) |
Cited By (3)
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---|---|---|---|---|
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US20120027649A1 (en) * | 2010-08-02 | 2012-02-02 | Sam Bhatia | Mounting media device |
EP3085317A1 (en) * | 2015-04-23 | 2016-10-26 | Shivani Scientific Industries Private Limited | Oocyte recovery apparatus and method of its use |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3750460B2 (en) * | 2000-02-18 | 2006-03-01 | 日立工機株式会社 | Dispensing device and dispensing method |
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Citations (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3982899A (en) * | 1975-05-22 | 1976-09-28 | Corning Glass Works | Fluid handling apparatus |
US4263257A (en) * | 1978-09-08 | 1981-04-21 | Metsaele Seppo Juhani | Pipette |
US4268481A (en) * | 1979-03-23 | 1981-05-19 | Kommandittiyhtio Finnpipette Osmo A. Suovaniemi | Pipette |
US4971763A (en) * | 1989-02-14 | 1990-11-20 | Eastman Kodak Company | Liquid-controlling nozzle geometry for dispensers of liquids |
US4973450A (en) * | 1987-06-10 | 1990-11-27 | Hoyer Gmbh And Company | Device for urinalysis |
US4988481A (en) * | 1989-01-30 | 1991-01-29 | Labsystems Oy | Electrical pipette |
US5204268A (en) * | 1988-06-02 | 1993-04-20 | Fuji Photo Film Co., Ltd. | Method and apparatus for applying liquid samples |
US5496272A (en) * | 1993-06-04 | 1996-03-05 | Kwahak International Co., Ltd. | Artificial insemination and embryo transfer device |
US5593893A (en) * | 1994-03-15 | 1997-01-14 | Toa Medical Electronics Co., Ltd | Dispensing device with syringe driving compensator for flexible tube |
US5629201A (en) * | 1992-05-22 | 1997-05-13 | Priolion Development B.V. | Apparatus for applying a liquid sample onto a culture medium |
US5707588A (en) * | 1994-04-05 | 1998-01-13 | Mitsubishi Denki Kabushiki Kaisha | Droplet floating apparatus |
US5736105A (en) * | 1991-02-01 | 1998-04-07 | Astle; Thomas W. | Method and device for simultaneously transferring plural samples |
US5770158A (en) * | 1996-06-13 | 1998-06-23 | Diametrics Medical, Inc. | Capillary syringe |
US5770160A (en) * | 1995-09-15 | 1998-06-23 | Bio-Plas, Inc. | Positive displacement liquid drawing and dispensing apparatus |
US5785926A (en) * | 1995-09-19 | 1998-07-28 | University Of Washington | Precision small volume fluid processing apparatus |
US5879944A (en) * | 1995-09-04 | 1999-03-09 | Fuji Photo Film Co., Ltd. | Liquid spotting method and liquid spotting device |
US5916524A (en) * | 1997-07-23 | 1999-06-29 | Bio-Dot, Inc. | Dispensing apparatus having improved dynamic range |
US6045759A (en) * | 1997-08-11 | 2000-04-04 | Ventana Medical Systems | Fluid dispenser |
US6258324B1 (en) * | 1999-03-15 | 2001-07-10 | Felix H. Yiu | Pipette dispensing block |
US6325114B1 (en) * | 2000-02-01 | 2001-12-04 | Incyte Genomics, Inc. | Pipetting station apparatus |
US6399024B1 (en) * | 2000-02-01 | 2002-06-04 | Incyte Genomics, Inc. | Multichannel pipette head |
US6516977B2 (en) * | 1996-02-21 | 2003-02-11 | Kwan-Ho Chan | System and method for mixing bone cement |
US6544479B1 (en) * | 1997-12-29 | 2003-04-08 | Thomas W. Astle | Piston seal for a pipettor |
US6627160B2 (en) * | 2000-03-20 | 2003-09-30 | Brand Gmbh + Co. Kg | Multiple channel pipetting device |
USRE38281E1 (en) * | 1996-07-26 | 2003-10-21 | Biodot, Inc. | Dispensing apparatus having improved dynamic range |
US20040208793A1 (en) * | 2001-05-09 | 2004-10-21 | Kari Jarvimaki | Pipette with tip container |
US6846680B2 (en) * | 2001-07-31 | 2005-01-25 | Caliper Life Sciences, Inc. | Liquid handling system with automatically interchangeable cannula array |
US6871557B2 (en) * | 2000-02-03 | 2005-03-29 | Rainin Instrument, Llc | Pipette device with tip ejector utilizing stored energy |
US20050142038A1 (en) * | 2003-12-31 | 2005-06-30 | Rainin Instrument, Llc | Volume adjustable manual pipette with quick set volume adjustment |
US6923938B2 (en) * | 2001-10-16 | 2005-08-02 | Matrix Technologies Corporation | Hand-held pipettor |
US6926867B1 (en) * | 2001-10-30 | 2005-08-09 | World Precision Instruments, Inc. | Pipette piston seal assembly |
US20050244303A1 (en) * | 2000-11-17 | 2005-11-03 | Tecan Trading Ag | Device for aspirating and dispensing liquid samples |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE8909141U1 (en) * | 1989-07-28 | 1990-08-30 | Zech, Herbert, Dr.Med., Bludenz, At | |
US6083762A (en) * | 1996-05-31 | 2000-07-04 | Packard Instruments Company | Microvolume liquid handling system |
US6063339A (en) * | 1998-01-09 | 2000-05-16 | Cartesian Technologies, Inc. | Method and apparatus for high-speed dot array dispensing |
US6269846B1 (en) * | 1998-01-13 | 2001-08-07 | Genetic Microsystems, Inc. | Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays |
US6133045A (en) * | 1998-02-27 | 2000-10-17 | Hamilton Company | Automated sample treatment system: apparatus and method |
US6033911A (en) * | 1998-02-27 | 2000-03-07 | Hamilton Company | Automated assaying device |
CN1310980A (en) * | 2000-02-29 | 2001-09-05 | 迪亚西斯公司 | Method and equipment for treating various kinds of liquid |
-
1997
- 1997-10-14 IL IL12196897A patent/IL121968A0/en unknown
-
1998
- 1998-10-14 EP EP98949221A patent/EP1121060A1/en not_active Withdrawn
- 1998-10-14 US US09/529,065 patent/US6420186B1/en not_active Expired - Fee Related
- 1998-10-14 WO PCT/IL1998/000498 patent/WO1999018872A1/en not_active Application Discontinuation
-
2002
- 2002-06-25 US US10/178,271 patent/US20020159921A1/en not_active Abandoned
Patent Citations (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3982899A (en) * | 1975-05-22 | 1976-09-28 | Corning Glass Works | Fluid handling apparatus |
US4263257A (en) * | 1978-09-08 | 1981-04-21 | Metsaele Seppo Juhani | Pipette |
US4268481A (en) * | 1979-03-23 | 1981-05-19 | Kommandittiyhtio Finnpipette Osmo A. Suovaniemi | Pipette |
US4973450A (en) * | 1987-06-10 | 1990-11-27 | Hoyer Gmbh And Company | Device for urinalysis |
US5204268A (en) * | 1988-06-02 | 1993-04-20 | Fuji Photo Film Co., Ltd. | Method and apparatus for applying liquid samples |
US4988481A (en) * | 1989-01-30 | 1991-01-29 | Labsystems Oy | Electrical pipette |
US4971763A (en) * | 1989-02-14 | 1990-11-20 | Eastman Kodak Company | Liquid-controlling nozzle geometry for dispensers of liquids |
US5736105A (en) * | 1991-02-01 | 1998-04-07 | Astle; Thomas W. | Method and device for simultaneously transferring plural samples |
US5629201A (en) * | 1992-05-22 | 1997-05-13 | Priolion Development B.V. | Apparatus for applying a liquid sample onto a culture medium |
US5496272A (en) * | 1993-06-04 | 1996-03-05 | Kwahak International Co., Ltd. | Artificial insemination and embryo transfer device |
US5593893A (en) * | 1994-03-15 | 1997-01-14 | Toa Medical Electronics Co., Ltd | Dispensing device with syringe driving compensator for flexible tube |
US5707588A (en) * | 1994-04-05 | 1998-01-13 | Mitsubishi Denki Kabushiki Kaisha | Droplet floating apparatus |
US5879944A (en) * | 1995-09-04 | 1999-03-09 | Fuji Photo Film Co., Ltd. | Liquid spotting method and liquid spotting device |
US5770160A (en) * | 1995-09-15 | 1998-06-23 | Bio-Plas, Inc. | Positive displacement liquid drawing and dispensing apparatus |
US5785926A (en) * | 1995-09-19 | 1998-07-28 | University Of Washington | Precision small volume fluid processing apparatus |
US6516977B2 (en) * | 1996-02-21 | 2003-02-11 | Kwan-Ho Chan | System and method for mixing bone cement |
US5770158A (en) * | 1996-06-13 | 1998-06-23 | Diametrics Medical, Inc. | Capillary syringe |
USRE38281E1 (en) * | 1996-07-26 | 2003-10-21 | Biodot, Inc. | Dispensing apparatus having improved dynamic range |
US5916524A (en) * | 1997-07-23 | 1999-06-29 | Bio-Dot, Inc. | Dispensing apparatus having improved dynamic range |
US6045759A (en) * | 1997-08-11 | 2000-04-04 | Ventana Medical Systems | Fluid dispenser |
US6416713B1 (en) * | 1997-08-11 | 2002-07-09 | Ventana Medical Systems, Inc. | Fluid dispenser |
US6544479B1 (en) * | 1997-12-29 | 2003-04-08 | Thomas W. Astle | Piston seal for a pipettor |
US6258324B1 (en) * | 1999-03-15 | 2001-07-10 | Felix H. Yiu | Pipette dispensing block |
US6325114B1 (en) * | 2000-02-01 | 2001-12-04 | Incyte Genomics, Inc. | Pipetting station apparatus |
US6399024B1 (en) * | 2000-02-01 | 2002-06-04 | Incyte Genomics, Inc. | Multichannel pipette head |
US6871557B2 (en) * | 2000-02-03 | 2005-03-29 | Rainin Instrument, Llc | Pipette device with tip ejector utilizing stored energy |
US6627160B2 (en) * | 2000-03-20 | 2003-09-30 | Brand Gmbh + Co. Kg | Multiple channel pipetting device |
US20050244303A1 (en) * | 2000-11-17 | 2005-11-03 | Tecan Trading Ag | Device for aspirating and dispensing liquid samples |
US20040208793A1 (en) * | 2001-05-09 | 2004-10-21 | Kari Jarvimaki | Pipette with tip container |
US6846680B2 (en) * | 2001-07-31 | 2005-01-25 | Caliper Life Sciences, Inc. | Liquid handling system with automatically interchangeable cannula array |
US6923938B2 (en) * | 2001-10-16 | 2005-08-02 | Matrix Technologies Corporation | Hand-held pipettor |
US6926867B1 (en) * | 2001-10-30 | 2005-08-09 | World Precision Instruments, Inc. | Pipette piston seal assembly |
US20050142038A1 (en) * | 2003-12-31 | 2005-06-30 | Rainin Instrument, Llc | Volume adjustable manual pipette with quick set volume adjustment |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070085772A1 (en) * | 2005-10-13 | 2007-04-19 | Park Kirack | Plasma display apparatus and method of driving the same |
US20120027649A1 (en) * | 2010-08-02 | 2012-02-02 | Sam Bhatia | Mounting media device |
EP3085317A1 (en) * | 2015-04-23 | 2016-10-26 | Shivani Scientific Industries Private Limited | Oocyte recovery apparatus and method of its use |
Also Published As
Publication number | Publication date |
---|---|
US6420186B1 (en) | 2002-07-16 |
WO1999018872A1 (en) | 1999-04-22 |
EP1121060A1 (en) | 2001-08-08 |
IL121968A0 (en) | 1998-03-10 |
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Legal Events
Date | Code | Title | Description |
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STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |