US20080249030A1

US20080249030A1 - Age Inhibitors

Info

Publication number: US20080249030A1
Application number: US11/909,761
Authority: US
Inventors: Pierre Potier; Marie-Claude Denise Michele Zelveyan; Catharine Marie Germaine Magnan
Original assignee: Centre National de la Recherche Scientifique CNRS; PHARMAMENS
Current assignee: Centre National de la Recherche Scientifique CNRS
Priority date: 2005-03-31
Filing date: 2006-03-30
Publication date: 2008-10-09
Also published as: FR2883873B1; US20120252866A1; JP2008534557A; CA2603386C; KR101343929B1; WO2006103274A9; BRPI0609783A8; WO2006103274A8; CN101268093A; AU2006228163A1; ATE540051T1; MA29416B1; KR20130110222A; JP4695186B2; US9512176B2; MX2007012238A; FR2883873A1; GEP20104882B; ZA200708835B; EP1863832A1

Abstract

The invention relates to a compound having general formula I, wherein: X represents CH₂, C═O, C═S or CHOH, X represents CH₂, C═O, C═S or CHOH, R₁represents an amino acid which is optionally substituted by one or more halogen atoms, preferably fluorine, or by one or more CF₃groups and n=0.1 or 2, or X represents CH₂, C═O, C═S, CHOH, R₁represents a peptide containing two amino acids, each amino acid being optionally substituted by one or more halogen atoms, preferably fluorine, or by one or more CF₃groups and n=0 or 1, or XR₁represent PO₃H or SO₃H and n=0.1 or 2; R₂represents H, XR₁, an alkyl group at C₁-C₆, an aralkyl group at C₁-C₆or an aryl group, whereby the alkyl, aralkyl and aryl groups can be substituted by an amine NH₂, a carboxylic group COOH, one or more halogen atoms, preferably fluorine, or one or more CF₃groups; or the pharmaceutically-acceptable addition salts, isomers, enantiomers and diastereoisomers of said compound, mixtures thereof, and pharmaceutical or cosmetic compositions comprising same and the use thereof as an AGE-inhibitor drug that traps reactive carbonyl compounds.

Description

The Maillard reaction, non-enzymatic glycation, is initiated by the condensation of an amino group present in proteins with a compound containing a carbonyl group, generally a sugar. A multitude of products, referred to as “advanced glycation end-products” (AGEs), result from the latter stages of this complex process. The consequence of the formation of these AGEs is protein cross-linking. Such cross-links have been observed in long-lived proteins such as collagen, lens crystalline, fibronectin, tubulin, myelin, laminin, actin, hemoglobin, albumin and the lipids associated with low-density lipoproteins (LDLs). AGE-modified proteins increase progressively with age and it is believed that they contribute to the normal tissue remodeling. Moreover, enhanced formation and accumulation of AGEs have been linked to the development of cataracts (Nagaraj et al., J. Biol. Chem. (1996) 271, 19338), uraemia (Miyata et al., Kidney Int. (1999) 55, 389), atherosclerosis (Kume et al., Am. J. Pathol. (1995) 147, 654; Stitt et al., Mol. Med. (1997) 3, 617), Alzheimer's disease (Münch et al., Biochem. Soc. Trans. (2003) 31 (6), 1397; Lüth et al., Cerebral Cortex (2005) 15(2), 211), Parkinson's disease (Webster et al., Neurotoxicity Res. (2005)/(172), 95), inflammatory disease (Anderson et al., J. Clin. Invest. (1999) 104, 103), age-related rheumatic disorders and, above all, clinical complications of diabetes mellitus (Brownlee, M. Ann. Rev. Med (1995) 461, 223; Brinkmann et al., J. Biol Chem. (1998) 273, 18714). Diabetic patients whose glycemia is elevated and persistent have an increased level of cross-linked proteins, which leads to tissue damage via modification of the structure and function of the proteins involved. Moreover, AGEs bind to membrane receptors and stimulate cellular responses. Since Maillard's discovery at the beginning of the last century, it has been believed that glucose is the sugar that participates in the cross-linking reaction. More recently, however, attention has been focused on α-dicarbonyl compounds, such as methylglyoxal (MG), glyoxal (GO) and 3-deoxyglucosone (3-DG), as active crosslinkers in vivo and in vitro. It is believed that the principal source of MG is the non-enzymatic dephosphorylation of triose-dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, which are glucose metabolites. MG can also be formed by the spontaneous decomposition of triose phosphates or by the metabolism of threonine or acetone. Some studies have also confirmed the generation of α-dicarbonyls via glucose auto-oxidation. It is believed that α-dicarbonyls can be generated during the transformation of a ketoamine, known as the Amadori product, a key intermediate in the Maillard reaction. This ketoamine is itself generated by the transformation of the Schiff-base adduct, which is initially formed during the reaction of glucose with an amine. In addition, it has been reported that bacteria produce MG. Lipid peroxidation of polyunsaturated fatty acids also yields reactive carbonyl compounds, such as MG and GO and those characteristic of lipids, such as malondialdehyde (MDA) and 4-hydroxynonenal. In general, such highly reactive dicarbonyls bind to the amino, guanidine and sulfhydryl groups of proteins and irreversibly form AGEs such as N_ε-(1-carboxyethyl)lysine (CEL), N_ε-(1-carboxymethyl)lysine (CML), methylglyoxal-derived hydroimidazolone N_δ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H₁), glyoxal-derived hydroimidazolone (G-H₁), argpyrimidine, glyoxal-derived lysine dimer, 1,3-di(N^ε-lysino)imidazolium salt (GOLD), and methylglyoxal-derived lysine dimer, 1,3-di(N′-lysino)-4-methylimidazolium salt (MOLD). The in vivo mechanism of action of these α-dicarbonyl compounds has been studied in an effort to understand the progression of the Maillard reaction in the organism. In diabetic subjects, increased formation and accumulation of AGEs occurs, thus leading to a series of long-term complications of diabetes such as nephropathy, retinopathy, neuropathy, ulcers and microvascular and macrovascular complications (Bucala et al., Diabetes Reviews (1995) 3, 258; Ulrich et al., Recent Prog. Horm. Res. (2001) 56, 1; Porta et al., Diabetologia (2002) 45, 1617; Lorenzi et al., Diabetologia (2001) 44, 791; Ziegler et al., Int. Rev. Neurobiol. (2002) 50, 451; Thornallay, P. J. Int. Rev. Neurobiol. (2002) 50, 37; Chiarelli et al., Diab. Nutr. Metab. (2000) 13, 192). More particularly, renal tissue damage caused by AGEs leads to the progressive loss of renal functioning (Makita Z., et al., N. Eng. J. Med. (1991) 325, 836). Indeed, among diabetic patients (type 1 and type 2), plasma concentration of methylglyoxal proved to be two to six times higher than that of normal subjects (McLellan et al., Clin. Sci. (1994) 87, 21).
Oxidative stress is another factor associated with ageing and with the current criteria for chronic diseases such as diabetes, atherosclerosis and related vascular diseases, rheumatoid polyarthritis and uremia. Oxidative stress is defined as a significant imbalance between antioxidant and oxidant generation systems. An increase in oxidative stress can have a profound effect on the modification of lipoproteins and on transcription, as well as on the functioning and metabolism of cells. Oxidative stress can appear via several mechanisms associated with the overproduction of oxygen radicals, such as the auto-oxidation of glucose and of glycated proteins and the glycation of antioxidant enzymes. Indeed, it has been reported that MG generates reactive oxygen species (ROS) (free radicals) during glycation reactions. Thus, it can be said that oxidative stress and AGE formation are inseparably intertwined.
Normally, the glyoxalase system (glyoxalase I and glyoxalase II) and aldose reductase catalyze the detoxification of these α-dicarbonyls into D-lactate, glycolate and acetol. However, a dysfunction of this detoxification metabolism leads to an increase in the quantity of AGEs formed by highly reactive α-dicarbonyls in the organism.
Inhibition of AGE formation can delay the progression of the physiopathology of AGE-related diseases and improve quality-of-life during ageing. It can thus be assumed that the pharmacological scavenging of α-dicarbonyl compounds is a valuable therapeutic strategy in the prevention of complications of diabetes. A large number of documents exist concerning the fact that an early stage pharmacological intervention against the long-term consequences of cross-linking prevents the development of later complications of diabetes. Even if AGE-formation inhibitors can not cure the underlying pathological process, they should delay the development of complications resulting from the fundamental disorders. Among the drugs specifically developed as AGE-formation inhibitors, aminoguanidine (pimagedine, AG) is the most studied and most used agent. AG is a nucleophilic compound with two key reactive functions, namely the nucleophilic hydrazine function —NHNH₂and the α-dicarbonyl directing guanidine function —NH—C(═NH)NH₂. These two functional groups bound together jointly form a reactive bifunctional scavenger of methylglyoxal, glyoxal and 3-desoxyglucosone (Brownlee, et al., Science (1986) 232, 1629). Although the beneficial effects of AG against the complications of diabetes have been largely confirmed in the diabetic rat model, AG is a well-known selective inhibitor of nitrogen monoxide (NO) and a clinical trial related to the prevention of the progression of diabetic nephropathy by AG was abandoned due to safety concerns (Oturai et al., APMIS (1996) 104, 259; Monnier, V. M. Arch. Biochem. Biophys. (2003) 419, 1). Pyridoxamine (pyridon) is another agent able to prevent complications in the diabetic rat with greater effectiveness than that of aminoguanidine, and it is able to scavenge lipid peroxidation products and α-dicarbonyl compounds (Metz et al., Archives of Biochemistry and Biophysics (2003) 419, 41). Metformin, an antihyperglycemic drug widely used in the management of type 2 diabetes, also reduces levels of methylglyoxal and glyoxal both in vivo and in vitro by forming triazepinones (Beisswenger et al., Diabetes Metab. (2003) 29, 6895). However, AG proved to be a much better scavenger (by a factor of 450) of methylglyoxal compared with metformin (Battah et al., Intern. Congress Series 1245 (2002) 355). Other compounds possessing AGE-formation inhibitory activity include D-penicillamine (Wondrak Get al., Biochem. Pharmacol. (2002) 63, 361), LR-90, methylene bis(4,4′-(2-chlorophenylureidophenoxyisobutyric acid)) (Rahbar et al., Arch. Biochem. Biophys. (2003) 419, 63), thiamin (Benfotiamine) (Stracke et al., J. Exp. Clin. Endocrinol. Diabetes (2001) 109, 330), carnosine (β-alanyl-L-histidine), a natural dipeptide widely distributed throughout mammalian tissues (Hipkiss A. R., Int. J. Biochem. Cell Biol. (1998) 30, 863), curcumin (Sajithlal et al., G. Biochem. Pharmacol. (1998) 56, 1607) another natural compound isolated from Curcuma longa, 2,3-diaminophenazine (NNC39-0028) (Soulis, et al., Diabetologia (1999) 42, 472). Given the marked impact of AGEs on quality-of-life during ageing, there remains a need to develop efficient agents that can scavenge highly reactive α-dicarbonyl compounds such as methylglyoxal, glyoxal and 3-desoxyglucosone and that have low cytotoxicity and low mutagenicity.
In a surprising way, the present inventors have discovered a new class of compounds able to inhibit the formation of advanced glycation end-products by scavenging reactive α-dicarbonyl compounds.
Some of these compounds are already known as such but not with respect to their therapeutic application.
Thus, patent application WO02/100344 discloses the synthesis intermediate
the article by Jones et al., (Tetrahedron Letters (1988), 29 (31), pages 3856-3856) discloses the synthesis intermediates
the article by Kasina et al., (Journal of Medicinal Chemistry (1986), 29 (10), pages 1933-1940) discloses the synthesis intermediate
the article by Tada et al., (Journal of Agricultural And Food Chemistry (1984), 32 (5), pages 992-996) discloses the flavor of peptides of formula I wherein R₂represents a hydrogen atom, X represents C═O, R₁represents —NH—(CH₂)_m—COOH and m=1, 2 or 3; the article by Shinoda et al., (Peptide Chemistry (1984), volume date 1983, 21st, pages 43-46) discloses the peptides and
and
having a salty flavor.
Only the patent application WO 2004/002418 discloses the peptide of formula
and its therapeutic application. This document does not, however, indicate that this peptide is an AGE inhibitor.
Additionally, derivatives analogous to those discovered by the inventors, in particular 2,3-diaminopropionic acid (DAPA), have been disclosed in a patent application (WO 92/14456). DAPA would be highly susceptible to decarboxylation by ornithine decarboxylase, a ubiquitous enzyme which participates in the synthesis of a large number of polyamines leading to ethylenediamine and/or 2-aminoacetamide. With a view to facilitate the elimination via the urine of the condensation products of α-dicarbonyl compounds and the scavenging agents, the presence of an acid functional group such as —COOH or SO₃H in the scavenger molecules is a crucial requirement. Otherwise, the condensation products would remain in circulation by renal tubular reabsorption mechanisms with the risk of a release α-dicarbonyls following another metabolic reaction. From the point of view of ornithine decarboxylase metabolism, the compounds discovered by the inventors of the present application can be used as agents, which are more effective than DAPA, to scavenge reactive α-dicarbonyl compounds such as methylglyoxal, glyoxal and 3-desoxyglucosone by forming adducts which are eliminated in the urine. Indeed, DAPA prevents the modification of insulin by MG, as is illustrated in FIG. 1. However, FIG. 7 demonstrates that its cytotoxicity is higher and that its effectiveness in protecting cells lower (68% cell survival when incubated with MG) compared with L-DAPA-L-Val (93%), L-DAPA-L-Leu (81%) and L-DAPA-L-Ile (79%.), compounds according to the present invention. In addition, DAPA appears to be mutagenic.
Thus, the present invention relates to a compound of following general formula I:
wherein:

X represents CH₂, C═O, C═S or CHOH, R₁represents an amino acid, optionally substituted by one or more halogen atoms, advantageously fluorine, or by one or more CF₃groups, and n=0, 1 or 2
or X represents CH₂, C═O, C═S or CHOH, R₁represents a peptide containing two amino acids, each amino acid being optionally substituted by one or more halogen atoms, advantageously fluorine, or one or more CF₃groups, and n=0 or 1
0or XR₁represents PO₃H or SO₃H and n=0, 1 or 2;
R₂represents H, XR₁, a C₁-C₆alkyl group, a C₁-C₆aralkyl group or an aryl group, the alkyl, aralkyl and aryl groups being able to be substituted by an amine (NH₂), a carboxylic group (COOH), one or more halogen atoms, advantageously fluorine, or one or more CF₃groups;
or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same,
with the exception of compounds
- wherein R₂represents a hydrogen atom, X represents C═O, R₁represents —NH—(CH₂)_m—COOH and m=1, 2 or 3 and n=0, 1 or 2;
- represented by the following formulas:

and the compounds L-ornithyl-taurine, L-diaminobutyryl-taurine and L-diaminopropionyl taurine.
In the sense of the present invention, the term “C₁-C₆alkyl group” means any alkyl group of one to six carbon atoms, linear or branched. In particular, it can relate to a methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl and n-hexyl group.
In the sense of the present invention, the term “aryl group” means one or more aromatic rings of five to eight carbon atoms, possibly adjoining or fused. In particular, the aryl group can be a phenyl or naphthyl group, advantageously phenyl.
In the sense of the present invention, the term “aralkyl group” means any aryl group as defined above, linked via an alkyl group as defined above. In particular, a benzyl group is an aralkyl group.
In the sense of the present invention, the “pharmaceutically acceptable addition salt” of a compound means any salt that is pharmaceutically acceptable and that has the desired pharmacological activity of the parent compound. Such salts comprise:

(1) acid addition salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like; or formed with organic acids such as acetic acid, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, citric acid, ethane-sulfonic acid, fumaric acid, glucoheptonic acid, gluconic acid, glutamic acid, glycolic acid, hydroxynaphthoic acid, 2-hydroxyethanesulfonic acid, lactic acid, maleic acid, malic acid, mandelic acid, methanesulfonic acid, muconic acid, 2-naphtalenesulfonic acid, propionic acid, salicylic acid, succinic acid, dibenzoyl-L-tartaric acid, tartaric acid, p-toluenesulfonic acid, trimethylacetic acid, trifluoroacetic acid and the like; or
(2) salts formed when an acid proton present in the parent compound either is replaced by a metal ion, for example an alkali metal ion, an alkaline earth metal ion or an aluminum ion; or coordinates with an organic or inorganic base. Acceptable organic bases include diethanolamine, ethanolamine, N-methylglucamine, triethanolamine, tromethamine and the like. Acceptable inorganic bases include aluminum hydroxide, calcium hydroxide, potassium hydroxide, sodium carbonate and sodium hydroxide.

Advantageous pharmaceutically acceptable salts are salts formed from hydrochloric acid, trifluoroacetic acid, dibenzoyl-L-tartaric acid and phosphoric acid.
It should be understood that all references to pharmaceutically acceptable salts include the solvent addition forms (solvates) or the crystalline forms (polymorphs), as defined herein, of the given acid addition salt.
The stereochemistry of the C-1 position of formula I (the carbon atom at the junction of the NH₂and X groups) can be R or S or a mixture thereof. The stereochemistry of the C-2 position (the carbon atom at the junction of the NH₂and R₂groups) can be R or S or a mixture thereof.
In the sense of the present invention, “amino acids” means all natural α-amino acid residues (for example alanine (Ala), arginine (Arg), asparagine (Asn), aspartic acid (Asp), cysteine (Cys), glutamine (Gln), glutamic acid (Glu), glycine (Gly), histidine (His), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), phenylalanine (Phe), proline (Pro), serine (Ser), threonine (Thr), tryptophane (Trp), tyrosine (Tyr) and valine (Val)) in D or L form, as well as non-natural amino acids (for example, β-alanine, allylglycine, tert-leucine, norleucine (Nle), 3-amino-adipic acid, 2-aminobenzoic acid, 3-aminobenzoic acid, 4-aminobenzoic acid, 2-aminobutanoic acid, 4-amino-1-carboxymethyl piperidine, 1-amino-1-cyclobutanecarboxylic acid, 4-aminocyclohexaneacetic acid, 1-amino-1-cyclohexanecarboxylic acid, (1R,2R)-2-aminocyclohexanecarboxylic acid, (1R,2S)-2-aminocyclohexanecarboxylic acid, (1S,2R)-2-aminocyclohexanecarboxylic acid, (1S,2S)-2-aminocyclohexanecarboxylic acid, 3-aminocyclohexanecarboxylic acid, 4-aminocyclohexanecarboxylic acid, (1R,2R)-2-aminocyclopentanecarboxylic acid, (1R,2S)-2-aminocyclopentanecarboxylic acid 1-amino-1-cyclopentanecarboxylic acid, 1-amino-1-cyclopropanecarboxylic acid, 4-(2-aminoethoxy)-benzoic acid, 3-aminomethylbenzoic acid, 4-aminomethylbenzoic acid, 2-aminobutanoic acid, 4-aminobutanoic acid, 6-aminohexanoic acid, 1-aminoindane-1-carboxylic acid, 4-aminomethyl-phenylacetic acid, 4-aminophenylacetic acid, 3-amino-2-naphthoic acid, 4-aminophenylbutanoic acid, 4-amino-5-(3-indolyl)-pentanoic acid, (4R,5S)-4-amino-5-methylheptanoic acid, (R)-4-amino-5-methylhexanoic acid, (R)-4-amino-6-methylthiohexanoic acid, (S)-4-amino-pentanoic acid, (R)-4-amino-5-phenylpentanoic acid, 4-aminophenylpropionic acid, (R)-4-aminopimeric acid, (4R,5R)-4-amino-5-hyroxyhexanoic acid, (R)-4-amino-5-hydroxypentanoic acid, (R)-4-amino-5-(p-hydroxyphenyl)-pentanoic acid, 8-aminooctanoic acid, (2S,4R)-4-amino-pyrrolidine-2-carboxylic acid, (2S,4S)-4-amino-pyrrolidine-2-carboxylic acid, azetidine-2-carboxylic acid, (2S,4R)-4-benzyl-pyrrolidine-2-carboxylic acid, (S)-4,8-diaminooctanoic acid, tert-butylglycine, γ-carboxyglutamate, β-cyclohexylanine, citruline, 2,3-diamino propionic acid, hippuric acid, homocyclohexylalanine, moleucine, homophenylalanine, 4-hydroxyproline, indoline-2-carboxylic acid, isonipecotic acid, α-methyl-alanine, nicopetic acid, norvaline, octahydroindole-2-carboxylic acid, ornithine, penicillamine, phenylglycine (Phg), 4-phenyl-pyrrolidine-2-carboxylic acid, pipecolic acid, propargylglycine, 3-pyridinylalanine, 4-pyridinylalanine, 1-pyrrolidine-3-carboxylic acid, sarcosine, the statins, tetrahydroisoquinoline-1-carboxylic acid, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid, tranexamic acid, 4,4-difluoro proline, 4-fluoro proline, alpha-(3,4-difluorobenzyl)-proline, gamma-(3,4-difluorobenzyl)-proline, alpha-(trifluoromethyl)phenylalanine, hexafluoroleucine, 5,5,5-trifluoroleucine, 6,6,6-trifluoronorleucine, 2-(trifluoromethyl)leucine, 2-(trifluoromethyl)norleucine, 4,4,4-trifluorovaline, 4,4,4,4′,4′,4′-hexafluorovaline, pentafluorophenylalanine, 2,3-difluorophenylalanine, 2,4-difluorophenylalanine, 2,5-difluorophenylalanine, 2,6-difluorophenylalanine, 3,4-difluorophenylalanine, 3,5-difluorophenylalanine, 3,3-difluoro-3-(4-fluorophenyl)alanine, 2,3-difluorophenylglycine, 2,4-difluorophenylglycine, 2,5-difluorophenylglycine, 3,4-difluorophenylglycine, 4,4-difluoroethylglycine, 4,4,4-trifluoroethylglycine and hexafluoronorleucine). The term also includes natural and non-natural amino acids carrying a conventional amino protecting group (for example, an acetyl group, tert-butyloxycarbonyl, benzyloxycarbonyl or 9-fluorenylmethylcarbonyl), as well as natural and non-natural amino acids protected at the carboxylic end (advantageously by a C₁-C₁₈alkyl group, an ester, a phenyl amide or benzyl amide or an amide, which, respectively, give a carboxylic end of the following formula: —CO(C₁-C₁₈alkyl), —COO (C₁-C₁₈alkyl), —CONHphenyl, CONHbenzyl, or CONH₂). Advantageously, the amino acid according to the present invention has its carboxylic end unprotected.
Advantageously, the amino acid according to the present invention has its carboxylic end protected in the form of a C₁-C₁₈alkyl ester (—COO(C₁-C₁₈alkyl)), preferably a C₁₃-C₁₈alkyl ester (—COO(C₁₃-C₁₈alkyl)).
Advantageously, the amino acid is linked to the X radical of the compound of formula I by the N-terminal end. Advantageously, the bond thus formed is as follows: —X—NH—R, wherein R represents the remainder of the amino acid molecule.
Advantageously, the amino acid according to the present invention is substituted by one or more halogen atoms (Br, Cl, I or F), advantageously fluorine, or one or more CF₃groups. Advantageously, this substitution is present on the alkyl or aryl moiety of the amino acid. Even more advantageously, the nitrogen atom is not substituted. The principal advantages of substitution by a halogen atom, in particular by a fluorine atom, or by a CF₃group relate to the bioavailability of the compounds obtained and, in particular, to improvements in their cell membrane permeation and binding characteristics.
Advantageously, the amino acid is selected among alanine, valine, isoleucine, proline, leucine, phenylalanine, glycine, β-alanine, norleucine, aspartic acid, lysine, or tert-leucine, advantageously among alanine, valine, isoleucine, proline, phenylalanine, leucine, norleucine or tert-leucine.
Advantageously, the phenyl radical of phenylalanine is substituted by one or more halogen atoms, advantageously fluorine, or by one or more CF₃groups, advantageously in the para position, less advantageously in the ortho or meta position.
Advantageously, the butyl radical of norleucine is substituted by one or more halogen atoms, advantageously fluorine, or by one or more CF₃groups.
In the sense of the present invention, the term “C₁-C₁₈alkyl group” means any alkyl group of one to 18 carbon atoms, linear or branched. In particular, it can relate to a methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl and n-hexyl group.
In the sense of the present invention, the term “peptide comprising two amino acids” means any sequence of two amino acids as defined below or of peptidyl residues. The sequence can be linear or cyclic. For example, a cyclic peptide can be prepared or can result from the formation of a disulfide bridge between two cysteine residues in a sequence. Advantageously, the peptide is linked to the remainder of the compound of formula I by the N-terminal end. Peptide derivatives can be prepared by any conventional method (in solution or solid phase) known in the art, such as those described in the examples below. The peptide sequences specifically described in the present application are written with the amino end on the left and the carboxylic end on the right. Advantageously, the peptide is selected among Ala-Gly, Ala-Ala, Ala-Pro or Ala-Val, advantageously among L-Ala-Gly, L-Ala-L-Ala, L-Ala-L-Pro or L-Ala-L-Val.
Advantageously, the compound according to the present invention is such that X represents C═O, CH₂or C═S.
Advantageously, the compound according to the present invention is such that R₂represents H or XR₁, advantageously XR₁.
In a specific embodiment, the compound according to the present invention is such that R, represents an amino acid, advantageously selected among alanine, valine, isoleucine, proline, leucine, norleucine, phenylalanine or tert-leucine.
Advantageously, the compound according to the present invention is such that X is C═O, n=0, and R₁is alanine, valine, leucine, isoleucine, proline, norleucine, phenylalanine or tert-leucine.
Advantageously, the compound according to the present invention is such that R₂is XR₁or H and n=0.
In another specific embodiment of the invention, the compound according to the present invention is represented by the following general formula II:
wherein:

R₁represents —NH—R₃—(C═O) R₄or

wherein

R₃represents
- a C₁-C₁₂alkyl group, advantageously C₁-C₆, optionally substituted by one or more groups chosen among a halogen atom, advantageously fluorine, a —CF₃, phenyl, phenol, —COOH, amine or phenyl group substituted by one or more halogen atoms, advantageously fluorine, or by one or more CF₃groups;
- a phenyl group, optionally substituted by an amine, an OH group, one or more halogen atoms, advantageously fluorine, or one or more CF₃groups and
R₄represents OH, NH₂, a C₁-C₃₀alkoxy, advantageously C₁-C₂₀;
R₂represents H, COR₁, or a C₁-C₆alkyl group optionally substituted by one or more halogen atoms, advantageously fluorine, or by one or more CF₃groups; n=0, 1 or 2;
Y represents an oxygen or sulfur atom, advantageously an oxygen atom;
or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same.

In the sense of the present invention, the term “C₁-C₁₂alkyl group” means any alkyl group of one to 12 carbon atoms, linear or branched. In particular, it can relate to a methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl and n-hexyl group.
The term “C₁-C₃₀alkoxy” means any —O—R radical, wherein R is a C₁-C₃₀alkyl radical as defined herein. Examples of alkoxy radicals include, but are not limited to, methoxy, ethoxy, isopropoxy and the like.
Advantageously the compound according to the present invention is such that n=0.
Advantageously R₂═H or COR₁.
In a specific embodiment, the compound according to the present invention is selected among:
or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same.
The present invention also relates to the use of a compound according to the present invention to prevent the deterioration of proteins in foods.
Said foods can be of animal or plant origin. The compounds according to the present invention are administered in an effective quantity in said foods in order to prevent the deterioration and the degradation of proteins contained therein. Such a use increases the period during which the foods can be consumed and stored and preserves their nutritional and organoleptic qualities.
Additionally, the present invention relates to a pharmaceutical or cosmetic composition comprising a compound according to the present invention and a pharmaceutically or cosmetically acceptable excipient.
The present invention relates to a compound of following general formula I
wherein:

X represents CH₂, C═O, C═S or CHOH, R₁represents an amino acid, optionally substituted by one or more halogen atoms, advantageously fluorine, or by one or more CF₃groups, and n=0, 1 or 2
or X represents CH₂, C═O, C═S or CHOH, R₁represents a peptide containing two amino acids, each amino acid being optionally substituted by one or more halogen atoms, advantageously fluorine, or one or more CF₃groups, and n=0 or 1
or XR₁represents PO₃H or SO₃H and n=0, 1 or 2;
R₂represents H, XR₁, a C₁-C₆alkyl group, a C₁-C₆aralkyl group or an aryl group, the alkyl, aralkyl and aryl groups being able to be substituted by an amine (NH₂), a carboxylic group (COOH), one or more halogen atoms, advantageously fluorine, or one or more CF₃groups;
or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same,
with the exception of the compound

for use as a drug.
Advantageously, the compound according to the present invention for use as a drug is represented by the following general formula II:
wherein:

R₁represents NH—R₃—(C═O)R₄or

wherein

Advantageously, the compound according to the present invention for use as a drug is selected among
or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same.
In a specific embodiment, the drug according to the present invention is a scavenger of reactive carbonyl compounds, advantageously an inhibitor of the formation of advanced glycation end-products.
Advantageously, the drug according to the present invention is for the prevention and/or the treatment of a state or disease due to the formation of advanced glycation end-products or to the cross-linking of proteins, for the prevention and/or the treatment of the deleterious effects of the ageing of an organism, said effects being the formation of advanced glycation end-products or the cross-linking of proteins, or in a patient for the slowing or the stopping of the progression of complications resulting from diabetes, said complications resulting from the formation of advanced glycation end-products or from the cross-linking of proteins.
Advantageously, the drug according to the present invention is intended to treat, prevent and/or slow in a patient the progression of diseases chosen among rheumatoid polyarthritis, Alzheimer's disease, uremia, neurodegenerative diseases, atherosclerosis, microvascular and macrovascular complications of diabetes including diabetic retinopathy and renal failure due to diabetic nephropathy, microangiopathies and macroangiopathies, cataracts, amyloidosis associated with dialysis or with Alzheimer's disease, Parkinson's disease, gingivitis, cavities, bucco-dental conditions, diabetic ulcers, chronic renal failure, chronic renal dialysis, inflammatory diseases, age-related rheumatic disorders and porphyria and to treat early-stage cancers.
Even more advantageously, the drug according to the present invention is for administration by oral route.
Additionally, the present invention relates to the use of a compound of general formula I or II as defined above for the preparation of a drug that scavenges reactive carbonyl compounds, advantageously an inhibitor of the formation of advanced glycation end-products, advantageously for
the prevention and/or the treatment of a state or disease due to the formation of advanced glycation end-products or to the cross-linking of proteins, the prevention and/or the treatment of the deleterious effects of the ageing of an organism, said effects being the formation of advanced glycation end-products or the cross-linking of proteins, or in a patient for the slowing or the stopping of the progression of complications resulting from diabetes, said complications resulting from the formation of advanced glycation end-products or from the cross-linking of proteins;
to treat, prevent and/or slow in a patient the progression of diseases chosen among rheumatoid polyarthritis, Alzheimer's disease, uremia, neurodegenerative diseases, atherosclerosis, microvascular and macrovascular complications of diabetes including diabetic retinopathy and renal failure due to diabetic nephropathy, microangiopathies and macroangiopathies, cataracts, amyloidosis associated with dialysis or with Alzheimer's disease, Parkinson's disease, gingivitis, cavities, bucco-dental conditions, diabetic ulcers, chronic renal failure, chronic renal dialysis, inflammatory diseases, age-related rheumatic disorders and porphyria and to treat early-stage cancers.
The present invention also relates to a method for the prevention and/or the treatment of a state or disease due to the formation of advanced glycation end-products or to the cross-linking of proteins, the prevention and/or the treatment of the deleterious effects of the ageing of an organism, said effects being the formation of advanced glycation end-products or the cross-linking of proteins, or in a patient for the slowing or the stopping of the progression of complications resulting from diabetes, said complications resulting from the formation of advanced glycation end-products or from the cross-linking of proteins; for the treatment, prevention and/or slowing in a patient of the progression of diseases chosen among rheumatoid polyarthritis, Alzheimer's disease, uremia, neurodegenerative diseases, atherosclerosis, microvascular and macrovascular complications of diabetes including diabetic retinopathy and renal failure due to diabetic nephropathy, microangiopathies and macroangiopathies, cataracts, amyloidosis associated with dialysis or with Alzheimer's disease, Parkinson's disease, gingivitis, cavities, bucco-dental conditions, diabetic ulcers, chronic renal failure, chronic renal dialysis, inflammatory diseases, age-related rheumatic disorders and porphyria and to treat early-stage cancers; said method comprising the administration in a patient in need of such a treatment of an effective quantity of a compound of general formula I or II according to the present invention as defined above.
Thus, the present invention relates to a drug or a pharmaceutical composition comprising a compound according to the present invention.
Said compositions or drugs can be formulated for administration in mammals, including human being. Dosing varies according to the treatment and to the affection to be treated. Said compositions or drugs are provided in such a way as to be suitable for administration by the digestive or parenteral route.
In the pharmaceutical compositions or drugs of the present invention for oral, sublingual, subcutaneous, intramuscular, intravenous, transdermal, local or rectal administration, the active ingredient can be administered in unit dose forms, in a mixture with conventional pharmaceutical carriers, to animals or to humans. Suitable unit dose forms include forms for oral administration such as tablets, gelatin capsules, powders, granules and oral solutions or suspensions, sublingual and buccal administration forms, subcutaneous, intramuscular, intravenous, intranasal, intraocular, or rectal administration forms.
When a solid composition or drug is prepared in tablet form, the principal active ingredient is mixed with a pharmaceutical carrier such as gelatin, starch, lactose, magnesium stearate, talc, gum arabic or analogues. The tablets can be coated with sucrose or other suitable materials or the tablets can be treated so that they have extended or delayed activity and that they continuously release a predetermined quantity of the active ingredient.
A preparation in gelatin capsules is obtained by mixing the active ingredient with a diluent and by pouring the mixture obtained into soft or hard gelatin capsules.
A preparation in syrup or elixir form can contain the active ingredient along with a sweetener and an antiseptic, as well as a flavoring agent and an agent that provides a suitable color.
Water-dispersible powders or granules can contain the active ingredient in a mixture with dispersion, wetting or suspension agents, as well as with flavor correctors or sweeteners.
Suppositories, which are prepared with binders that melt at rectal temperature, such as cocoa butter or polyethylene glycol, are used for rectal administration.
For parenteral, intranasal or intraocular administration, suitable preparations include aqueous suspensions, isotonic saline solutions or sterile injectable solutions containing pharmacologically compatible dispersion and/or wetting agents.
The active ingredient can be also formulated in microcapsule form, optionally with one or more carrier additives.
The active ingredient can also be administered by topical route.
The present invention also relates to the cosmetic use of a compound according to the present invention as an anti-ageing and restructuring active ingredient for the epidermis and the papillary dermis and/or as an anti-wrinkle active ingredient.
The compounds according to the present invention have a tensor effect on the skin. They can be administered by oral or topical route.
The cosmetic or pharmaceutical compositions according to the present invention can be formulated for administration by topical route. They can be provided in the forms commonly used for this type of administration, i.e., notably lotions, foams, gels, dispersions, sprays, shampoos, serums, masks, body milks or creams, for example, with excipients enabling in particular cutaneous penetration in order to improve the properties and the accessibility of the active ingredient. The forms can be a single-phase vehicle comprised of a neutral hydroxypropylcellulose gel or a gel containing sodium carboxymethylcellulose. It is also possible to prepare creams and two-phase vehicles containing a hydrophilic phase dispersed in a lipophilic phase.
In addition to the composition according to the present invention, such compositions or drugs generally contain a physiologically acceptable medium, in general containing water or solvents such as alcohols, ethers or glycols, for example. They can also contain a cosmetically or pharmaceutically acceptable excipient. Such excipients can be selected among compounds exhibiting suitable compatibility with the active ingredient. Examples of such excipients include natural water-soluble polymers such as polysaccharides (xanthan gum, carob bean gum, peptin, etc.) or polypeptides, cellulose derivatives such as methylcellulose, hydroxypropylcellulose and hydroxypropyl-methylcellulose, as well as synthetic polymers, poloxamers, carbomers, PVA or PVP.
Lastly, a person skilled in the art may choose to add to this cosmetic or pharmaceutical composition various co-solvent excipients such as ethanol, glycerol, benzyl alcohol, humectants (glycerol), diffusion agents (Transcutol, urea) or antibacterial preservatives (0.15% methyl p-hydroxybenzoate). Said composition can also contain surfactants, stabilizers, emulsifiers, thickeners, other active ingredients providing a complementary or possibly synergistic effect, trace elements, essential oils, fragrances, colorants, collagen, chemical or mineral filters, hydrating agents or thermal spring water.
The present invention also relates to a method for the cosmetic anti-ageing treatment of the skin by the application of a composition comprising a compound according to the present invention.
The abbreviations used within the framework of this application are as follows: DAPA=2,3-diaminopropionic acid, DABA=2,3-diaminobutylic acid (absent specification to the contrary) or 2,4-diaminobutylic acid, DASA=diaminosuccinic acid, EDC=1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride, HOBt=1-hydroxybenzotriazole hydrate, Boc=t-butoxycarbonyl, TFA=trifluoroacetic acid, THF=tetrahydrofuran.

The present invention will be better understood in reference to the figures wherein:

FIG. 1 presents the comparative effects of dicarbonyl scavengers in the form of dihydrochloride according to the present invention and the comparison with DAPA on the modification of insulin (Ins) by methylglyoxal (MG). The percentage of insulin is indicated following incubation with methylglyoxal in the presence or absence of dicarbonyl scavengers. Insulin (0.034 mM) is incubated in vitro in a 10 mM phosphate buffer, pH 7.45 (containing 0.1 M NaCl) with methylglyoxal (3.4 mM) in the presence of dicarbonyl scavengers (4.08 mM) for 21 hours at 37° C. Insulin concentration is measured by HPLC (same conditions as in FIG. 3).

FIG. 2 presents the comparative effects of the dicarbonyl scavengers of the prior art on the modification of insulin by methylglyoxal. The experimental conditions are identical to those described in FIG. 1. The compounds marked with an asterisk (*) are used in hydrochloride form.

FIG. 3 presents the results obtained by HPLC for two compounds according to the present invention, L-DAPA-L-Leu (example 1) and L-DAPA-L-Val (example 23), on insulin modifications induced by methylglyoxal. It can be observed that these modifications are prevented. The HPLC conditions are as follows: C-18, Symmetry 300 (4.6×250 mm) column, injection volume 100 μl of reaction mixture; flow 1 ml/min; temperature 40° C.; solvent A: H₂O+0.1% TFA; solvent B: 60/40 CH₃CN/H₂O+0.1% TFA; linear gradient of 50% B to 55% B in 15 minutes; detection: TV at 215 nm: PDA (chromatograms extracted at 220 nm).

FIG. 4 presents the results obtained by HPLC for two compounds according to the present invention, L-DAPA-L-Leu (example 1) and L-DAPA-L-Val (example 23), on somatostatin-14 modifications induced by methylglyoxal. HPLC conditions are as follows: C-18, Symmetry 300 (4.6×250 mm) column, injection volume 100 μl of reaction mixture; flow 1 ml/min; solvent A: H₂O+0.1% TFA; solvent B: 80/20 CH₃CN/H₂O+0.1% TFA; linear gradient: from 20% B to 60% B in 15 minutes and isocratic from 60% B for 10 minutes; ambient temperature; detection: PDA (chromatograms extracted at 215 nm). The compounds according to the present invention prevent the modifications induced by methylglyoxal.

In this figure, (a) represents the results of somatostatin-14 alone; (b) represents the results of somatostatin-14+methylglyoxal; (c) represents the results of somatostatin-14+methylglyoxal+L-DAPA-L-Val (example 23) and (d) represents the results of somatostatin-14+methylglyoxal+L-DAPA-L-Leu (example 1). To obtain these results, somatostatin-14 (0.03 mm) is incubated in vitro in a 10 mM phosphate buffer, pH 7.45, containing 0.1 M NaCl, with or without (a) methylglyoxal (3.6 mM) in the presence ((c) and (d)) or the absence (b) of compounds according to the present invention (4.3 mM) for 24 hours at 37° C.

FIG. 5 presents the results obtained by HPLC for three compounds according to the present invention, L-DAPA-L-Ile (example 18), L-DAPA-L-Val (example 23) and L-DAPA-L-Leu (example 1), on RNase A modifications induced by methylglyoxal. HPLC conditions are as follows: C-18, Symmetry 300 (4.6×250 mm) column, injection volume 100 μl of reaction mixture diluted to 1/10; flow 1 ml/min; temperature 40° C.; solvent A: H₂O+0.1% TFA; solvent B: 60/40 CH₃CN/H₂O+0.1% TFA; linear gradient of 206 B to 80% B in 20 minutes; detection: UV at 215 nm: PDA (chromatograms extracted at 215 nm). The compounds according to the present invention prevent the modifications to RNase A. In this figure: (a) represents the results of RNase A; (b) represents the results of RNase A+methylglyoxal; (c) represents the results of RNase A+methylglyoxal+L-DAPA-L-Ile (example 18); (d) represents the results of RNase A+methylglyoxal+L-DAPA-L-Val (example 23); (e) represents the results of RNase A+methylglyoxal+L-DAPA-L-Leu (example 1). To obtain these results, RNase A (0.08 mM) is incubated in vitro in a 100 mM phosphate buffer, pH 7.45, with or without (a) methylglyoxal (32 mM) in the presence ((c), (d) and (e)) or the absence (b) of compounds according to the present invention (38 mM) for 21 hours at 37° C.

FIGS. 6 and 7 represents EA endothelial cell growth in the presence of compounds according to the present invention and compounds according to the prior art, in particular aminoguanidine (AG) and diaminopropionic acid (DAPA), in the presence or absence of methylglyoxal (MG).

The general method for producing compounds according to the present invention comprises step (a), or steps (a) and (b), or steps (a), (b) and (c), or steps (a) and (d), or steps (a), (d) and (e), as follows:

a) coupling of an amino acid or peptide alkyl ester with an N-protected diamino acid (for example, DAPA, DABA, DASA, Orn or Lys according to the value of n) in an organic solvent, advantageously dichloromethane, advantageously by using reagents forming an active ester, such as EDC and HOBt, for example, advantageously under agitation at room temperature;

b) alkaline hydrolysis of the alkyl ester obtained in step (a), advantageously with LiOH, advantageously in the solvent THF/MeOH/H₂O, MeOH/H₂O or H₂O, then acidification, advantageously with an aqueous solution of KHSO₄at pH 5 to obtain the pure acid;

c) deprotection of the N-protecting groups of the acid obtained in step (b) advantageously with 3 M HCl-dioxane (or THF) and elimination of the volatile components;

d) preparation of thioamides by addition of Lawesson's reagent to the peptide obtained in step (a), advantageously under inert atmosphere, and heating, advantageously at 80° C., for two hours;

e) deprotection of the thioamides with di-Boc, tert-butyl ester protection obtained in step (d) by the addition of TFA in an organic solvent, advantageously dichloromethane, at a low temperature, advantageously 0° C.

In an advantageous embodiment, the compounds according to the present invention can be produced according to the method described hereafter, i.e., the implementation of step (1), or of steps (1) and (2), or of steps (1), (2) and (3), or of steps (1) and (4), or of steps (1), (4) and (5).

1. Coupling Reaction of N-Protected Carboxylic Acids and Amino Acid Alkyl Esters

To a solution of a diamino acid (for example, DAPA, DABA, DASA, Orn or Lys) (1.0 mmol) properly N-protected (preferably by a Boc group) and an amino acid alkyl ester (1.1 mmol) in dichloromethane (5.0 ml) were added reagents forming an active ester (for example, EDC (1.2 mmol) and HOBt (1.1 mmol)) and the reaction mixture was stirred at room temperature overnight. Water was added and the aqueous phase was extracted with EtOAc. The combined organic layers were washed successively with 1 N HCl, H₂O, saturated NaHCO₃and brine, dried on Na₂SO₄and then filtered. The solvent was evaporated under reduced pressure and then the residue was purified by flash column chromatography to obtain the dipeptide.

2. Alkaline Hydrolysis of the Alkyl Ester

To a solution of alkyl ester (1.0 mmol) in THF/MeOH/H₂O or MeOH/H₂O at room temperature was added an alkaline solution (preferably 1.0 mmol LiOH). The reaction mixture was then agitated until all of the starting ester had disappeared (approximately overnight). The reaction mixture was acidified with an aqueous solution of KHSO4 at pH 5 and then extracted with an organic solvent (preferably CH₂Cl₂). The organic phase was dried (Na₂SO₄) and then evaporated under reduced pressure to obtain the crude acid, which is used directly in the following reaction without additional purification.

3. Deprotection of the N-Protecting Groups

A solution of N-protected dipeptide carboxylic acid (1 mmol) in 3 M HCl-dioxane (or THF) was agitated at room temperature for three to nine hours. The volatile components were eliminated by evaporation to obtain the dipeptide hydrochloride.

4. Preparation of the Thioamides

Lawesson's reagent (1.1 mmol) was added all at once to a solution of the dipeptide mentioned above (step 1) (2.0 mmol) in toluene (10 ml) at room temperature under an argon atmosphere. The reaction mixture was agitated for two hours at 80° C. The solvent was eliminated by evaporation under reduced pressure. The residue was purified by silica-gel column chromatography (CH₂C₁₂then 10/1 CH₂C₁₂/Et₂O) to obtain the corresponding thioamide.

5. Deprotection of the Thioamides with di-Boc, Tert-Butyl Ester Protection

TFA (5 ml) was added to a solution of thioamide tert-butyl ester with di-Boc protection (1 mmol) in dichloromethane (5 ml) at 0° C.; the resulting solution was stored overnight at 0° C. The volatile components were eliminated by evaporation to obtain the dithiopeptide in the form of trifluoroacetic acid salt.

The following examples are given as non-limiting illustrations.

The following compounds according to the present invention were prepared by implementing the method described above.

EXAMPLE 1

L-DAPA-L-Leu.2HCl

¹H NMR (300 MHz, CD₃OD) δ 4.37 (t, J=5.8 Hz, 1H), 4.30 (dd, J=9.4, 5.6 Hz, 1H), 3.45 (dd, J=13.9, 6.0 Hz, 1H), 3.34 (dd, J=13.9, 5.3 Hz, 1H), 1.64-1.49 (m, 3H), 0.78 (d, J=6.4 Hz, 3H), 0.74 (d, J=6.4 Hz, 3H);
¹³C NMR (75 MHz, CD₃OD) δ 176.6, 167.3, 53.0, 51.7, 41.4, 40.6, 26.1, 23.4, 21.5;
MS (ESI) m/z 218 [M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H) 218.1505; found: 218.1512.
[α]_D ²⁶+3.41 (c 1.0, 6 N HCl)

EXAMPLE 2

L-DAPA-D-Leu.2HCl

[α]_D ²²+7.8 (c 1.0 H₂O)
[α]_D ²⁶+39.59 (c 1.0, 6 N HCl)
¹H NMR (300 MHz, CD₃OD) δ 4.38 (dd, J=5.0, 6.7 Hz, 1H), 4.30 (t, J=7.8 Hz, 1H), 3.60-3.43 (m, 2H), 1.67-1.56 (m, 3H), 0.86 (d, J=6.0 Hz, 3H), 0.83 (d, J=6.0 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 175.9, 166.0, 52.3, 50.7, 39.6, 39.1, 24.5, 22.0, 20.9;
MS (ESI) m/z 218 [M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H) 218.1505; found: 218.1552.

EXAMPLE 3

L-DAPA-L-Leu.2TFA

[α]_D ²²+20 (c 0.5, MeOH;
[α]_D ²⁴+1.13 (c 1.0, 6 N HCl)
¹H NMR (300 MHz, CD₃OD) δ 4.51-4.46 (m, 1H), 4.12 (t, J=6.2 Hz, 1H), 3.36 (d, J=5.9 Hz, 2H), 1.78-1.62 (m, 3H), 0.98 (d, J=6.1 Hz, 3H), 0.95 (d, J=6.1 Hz, 3H);
¹³C NMR (62.5 MHz, D₂O) δ 176.6, 166.8, 52.6, 51.1, 40.3, 39.7, 25.1, 22.7, 21.1;
MS (ESI) m/z 218 [M+H]⁺, 240 [M+Na]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H) 218.1505; found: 218.1512, calculated for C₉H₁₉N₃O₃Na (M+Na) 240.1324; found: 240.1364.

EXAMPLE 4

L-DAPA-L-LeuOMe.2TFA

[α]_D+3.8 (c 1.2, MeOH);
¹H NMR (300 MHz, CD₃OD) δ 4.55 (t, J=7.4 Hz, 1H), 4.42 (t, J=5.9 Hz, 1H), 3.75 (s, 3H), 3.52 (d, J=5.9 Hz, 2H), 1.81-1.61 (m, 3H), 0.95 (d, J=6.8 Hz, 3H), 0.93 (d, J=6.6 Hz, 3H)
¹³C NMR (62.5 MHz, CD₃OD) δ 175.0, 167.4, 53.4, 52.8, 51.9, 41.3, 40.9, 25.9, 23.3, 21.6;
MS (ESI) m/z 232 (M+H]⁺, 254 [M+Na]⁺;
HRMS calculated for C₁₀H₂₂N₃O₃(M+H) 232-1661; found: 232.1660.

EXAMPLE 5

L-DAPA-L-LeuOMe.2HCl

¹H NMR (300 MHz, CD₃OD) δ 4.46 (dd, J=7.7, 5.6 Hz, 1H), 4.44 (t, J=4.9 Hz, 1H), 3.67 (s, 3H), 3.48 (d, J=5.6 Hz, 2H), 1.75-1.55 (m, 3H), 0.89 (d, J=6.4 Hz, 3H), 0.86 (d, J=6.4 Hz, 3H);
¹³C NMR (75 MHz, CD₃OD) δ 175.0, 167.1, 53.4, 52.9, 51.8, 41.4, 40.8, 26.0, 23.3, 21.6;
MS (ESI) m/z 232 [M+H]⁺;
HRMS calculated for C₁₀H₂₂N₃O₃(M+H) 232.1661; found: 232.1660.
[α]_D ²⁴+6.2 (c 0.7, MeOH)

EXAMPLE 6

L-DAPA-L-IleNH₂.2HCl

[α]_D+25 (c 0.5, MeOH);
¹H NMR (300 MHz, CD₃OD) δ 4.59 (dd, J=7.0, 5.5 Hz, 1H), 4.37 (d, J=5.5 Hz, 1H), 3.57 (dd, J=13.4, 5.5 Hz, 1H), 3.36 (dd, J=13.4, 7.2 Hz, 1H), 2.01-1.91 (m, 1H), 1.58-1.47 (m, 1H), 1.44-1.28 (m, 1H), 1.05 (d, J=6.8 Hz, 3H), 0.95 (t, J=7.4 Hz, 3H);
¹³C NMR (62.5 MHz, CD₃OD) δ 176.3, 167.6, 60.6, 51.3, 41.2, 37.8, 25.5, 16.3, 12.0;
MS (ESI) m/z 217 [M+H]⁺, 239 [M+Na]⁺;
HRMS calculated for C₉H₂₁N₄O₂(M+H) 217.1665; found: 217.1674, calculated for C₉H₂₀N₄O₂Na (M+Na) 239.1484; found: 239.1499.

EXAMPLE 7

D-DAPA-D-Leu.2HCl

¹H NMR (300 MHz, D₂O) δ 4.38-4.31 (m, 2H), 3.50-3.34 (m, 2H), 1.62-1.50 (m, 3H), 0.79 (d, J=6.4 Hz, 3H), 0.75 (d, J=6.4 Hz, 3H).
[α]_D−11.7 (c 1.0, H₂O)
MS (ESI) m/z 218 [M+H]⁺; 240 [M+Na]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H): 218.1505; found: 218.1537

EXAMPLE 8

D-DAPA-D-Ala.2HCl

[α]_D−21.9 (c 1.0, MeOH);
[α]_D ²⁶−6.15 (c 1.0, 6 N HCl)
¹H NMR (300 MHz, D₂O) δ 4.53 (q, J=7.4 Hz, 1H), 4.48 (t, J=6.0 Hz, 1H), 3.64 (d, J=6.0 Hz, 2H), 1.50 (d, J=7.4 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 176.7, 166.5, 51.2, 49.9, 40.3, 16.6;
MS (ESI) m/z 176 [M+H]⁺;
Analysis, calculated for C₆H₁₅N₃O₃Cl₂: C, 29.05; H, 6.09; N, 16.94; Cl, 28.58; found: C, 28.67; H, 6.24; N, 16.67; Cl, 27.64.

EXAMPLE 9

L-DAPA-L-Ala.2HCl

¹H NMR (300 MHz, D₂O) δ 4.51 (q, J=7.5 Hz, 1H), 4.46 (t, J=6.0 Hz, 1H), 3.62 (d, J=6.0 Hz, 2H), 1.49 (d, J=7.5 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 176.8, 166.5, 51.2, 49.9, 40.3, 16.6;
MS (ESI) m/z 176 [M+H]⁺;
HRMS calculated for C₆H₁₄N₃O₃, (M+H) 176.1035; found: 176.1037.
[α]_D+7.83 (c 1.0, 6 N HCl)

EXAMPLE 10

L-DAPA-D-Ala.2HCl

¹H NMR (300 MHz, D₂O) δ 4.33-4.24 (m, 2H), 3.50-3.39 (m, 2H), 1.41 (d, J=7.4 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 176.0, 166.0, 50.9, 49.5, 39.8, 16.3;
MS (ESI) m/z 176 [M+H]⁺;
HRMS calculated for C₆H₁₄N₃O₃(M+H) 176.1035; found: 176.1044.
[α]_D ²⁶+64.56 (c 1.0, 6 N HCl)

EXAMPLE 11

D-DAPA-L-Ala.2HCl

¹H NMR (300 MHz, D₂O) δ 4.49-4.41 (m, 2H), 3.68-3.54 (m, 2H), 1.49 (d, J=7.3 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 176.4, 166.3, 51.3, 49.8, 40.1, 16.6;
MS (ESI) m/z 176 [M+H]⁺;
HRMS calculated for C₆H₁₄N₃O₃(M+H) 176.1035; found: 176.1043.
[α]_D ²⁶−60.9 (c 1.0, 6 N HCl)

EXAMPLE 12

(2S, 3S)-DABA-L-Leu.2HCl

¹H NMR (300 MHz, D₂O) δ 4.34 (t, J=7.2 Hz, 1H), 4.22 (d, J=4.2 Hz, 1H), 3.87 (dq, J=4.2, 7.2 Hz, 1H), 1.58-1.56 (m, 3H), 1.34 (d, J=6.8 Hz, 3H), 0.79 (d, J=6.0 Hz, 3H), 0.76 (d, J=6.0 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 175.9, 165.6, 54.4, 52.0, 47.9, 39.0, 24.3, 22.0, 20.5, 14.0;
MS (ESI) m/z 232 [M+H]⁺;
HRMS calculated for C₁₀H₂₂N₃O₃(M+H) 232.1661; found: 232.1663.
[α]_D ²²+9.6 (c 0.2, H₂O)

EXAMPLE 13

L-DAPA-Gly-OC₁₆H₃₃.2HCl

¹H NMR (300 MHz, CD₃OD) δ 4.44 (t, J=5.8 Hz, 1H), 4.20 (t, J=6.7 Hz, 2H), 4.17, 4.07 (AB q, J=17.8 Hz, 2H), 3.53 (d, J=5.8 Hz, 2H), 1.73-1.64 (m, 2H), 1.43-1.30 (m, 26 H), 0.91 (t, J=6.7 Hz, 3H);
¹³C NMR (75 MHz, CD₃OD) δ 171.6, 167.5, 67.0, 51.9, 42.3, 41.2, 33.1, 30.8, 30.7, 30.5, 30.4, 29.7;
MS (ESI) m/z 386 [M+H]⁺;
HRMS calculated for C₂₁H₄₄N₃O₃(M+H) 386.3383; found: 386.3352.
[α]_D ²⁶−5.98 (c 0.5, MeOH)

EXAMPLE 14

L-DAPA-L-Leu-OC₁₆H₃₃.2HCl

¹H NMR (300 MHz, CD₃OD) δ 4.34-4.28 (m, 2H), 4.01-3.86 (m, 2H), 3.41-3.29 (m, 2H) , 1.61-1.39 (m, 5H), 1.16-1.05 (m, 26H), 0.76 (d, J=6.4 Hz, 3H), 0.72 (d, J=6.4 Hz, 3H), 0.66 (t, J=6.7 Hz, 3H);
¹³C NMR (75 MHz, CD₃OD) δ 174.6, 167.2, 67.2, 53.0, 51.8, 41.4, 40.9, 33.2, 30.9, 30.6, 30.6, 30.6, 30.4, 29.7, 27.0, 26.1, 23.8, 23.4, 21.8, 14.6;
MS (ESI) m/z 442 [M+H]⁺;
HRMS calculated for C₂₅H₅₂N₃O₃(M+H) 442.4009; found: 441.3983.
[α]_D ²⁶+13.1 (c 2.0, MeOH)

EXAMPLE 15

L-DAPA-L-(S)-Leu.2TFA

¹H NMR (300 MHz, D₂O) δ 4.79 (dd, J=9.3, 5.4 Hz, 1H), 4.56 (t, J=6.4 Hz, 1H), 3.48 (dd, J=13.8, 5.8 Hz, 1H), 3.41 (dd, J=13.8, 6.6 Hz, 1H), 1.79-1.53 (m, 3H), 0.81 (d, J=6.4 Hz, 3H), 0.77 (d, J=6.4 Hz, 3H);
¹³C NMR (62.5 MHz, D₂O) δ 194.5, 174.9, 58.0, 54.6., 41.5, 38.8, 24.7, 22.0, 20.7;
MS(ESI) m/z 234 [M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₂S: 234.1276; found: 234.1306.
[α]_D ²⁶+60.6 (c 2.5, MeOH)

EXAMPLE 16

L-DAPA-L-(S)-Leu.2HCl

¹H NMR (300 MHz, D₂O) δ 4.80 (dd, J=9.6, 4.9 Hz, 1H), 4.60 (t, J=6.2 Hz, 1H), 3.52-3.39 (m, 2H), 1.81-1.58 (m, 3H), 0.82 (d, J=6.4 Hz, 3H), 0.78 (d, J=6.2 Hz, 3H);
¹³C NMR (62.5 MHz, D₂O) δ 194.5, 174.8, 57.9, 54.6, 41.5, 38.8, 24.7, 22.0, 20.7;
MS(ESI) m/z 234[M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₂S: 234.1276; found: 234.1306.
[α]_D ²⁶+90.6 (c 1.0, MeOH)

EXAMPLE 17

L-DAPA-Gly.2HCl

¹H NMR (300 MHz, D₂O) δ 4.54 (t, J=5.8 Hz, 1H), 4.20 (d, J=18.0 Hz, 1H), 4.10 (d, J=18.0 Hz, 1H), 3.65 (d, J=5.8 Hz, 1H);
¹³C NMR (75 MHz, D₂O) δ 173.1, 166.5, 50.5, 41.6, 39.5; MS (ESI) m/z 162[M+H]+
HRMS calculated for C₅H₁₂N₃O₃(M+H) 162.0879; found: 162.0864.
[α]_D ²⁵+28 (c 1.8, H₂O)

EXAMPLE 18

L-DAPA-L-Ile.2HCl

¹H NMR (300 MHz, D₂O) δ 4.52 (t, J=5.9 Hz, 1H); 4.46 (d, J=4.9 Hz, 1H); 3.60 (d, J=5.9 Hz, 2H); 2.05 (m, 1H); 1.45 (m, 1H); 1.27 (m, 1H); 0.97 (d, J=6.9 Hz, 3H); 0.90 (t, J=7.3 Hz, 3H);
¹³C NMR (62.5 MHz, D₂O) δ 175.5, 166.8, 58.8, 51.1, 40.3, 37.0, 25.3, 15.7, 11.6;
MS (ESI) m/z 218 [M+H], 240 [M+Na]⁺;
HRMS calculated for C₉H₂₀N₃O₃: 218.1505; found: 218.1537.
[α]_D ²⁶+22.4 (c 1.2, H₂O)

EXAMPLE 19

L-DAPA-P-Ala.2HCl

¹H NMR (300 MHz, CD₃OD) δ 4.40 (t, J=5.8 Hz, 1H), 3.54 (m, 4H); 2.64 (m, 2H);
¹³C NMR (62.5 MHz, CD₃OD) δ 174.0, 166.6, 52.1, 41.1, 36.9, 34.2;
MS (ESI) m/z 176 [M+H]⁺;
HRMS calculated for C₆H₁₄N₃O₃(M+H) 176.1035; found: 176.1068;
[α]_D ²⁶+3.8 (c 0.5, H₂O)

EXAMPLE 20

D-DAPA-β-Ala.2HCl

¹H NMR (300 MHz, CD₃OD) δ 4.40 (t, J=5.8 Hz, 1H), 3.54 (m, 4H); 2.64 (m, 2H);
¹³C NMR (62.5 MHz, CD₃OD) δ 174.0, 166.6, 52.1, 41.1, 36.9, 34.2;
MS (ESI) m/z 176 [M+H]⁺.

EXAMPLE 21

L-DAPA-L-Phe.2HCl

¹H NMR (300 MHz, CD₃OD) δ 7.32 (m, 5H); 4.79 (dd, J=9.9, 4.4 Hz, 1H); 4.48 (t, J=5.9 Hz, 1H); 3.61 (dd, J=13.9, 6.1 Hz, 1H); 3.51 (dd, J=13.9, 5.7 Hz, 1H); 3.35 (dd, J=14.2, 3.4 Hz, 1H); 3.08 (dd, J=14.2, 9.9 Hz, 1H);
¹³C NMR, (62.5 MHz, CD₃OD) δ 175.1, 167.2, 138.2, 130.3, 129.7, 128.1, 68.2, 56.2, 51.7, 41.3, 37.5;
MS (ESI) m/z 252 [M+H]⁺.
HRMS calculated for C₁₂H₁₈N₃O₃(M+H) 252.1348; found: 252.1341.
[α]_D ²⁶+41.8 (c 1.0, H₂O)

EXAMPLE 22

D-DAPA-D-Phe.2HCl

¹H NMR (300 MHz, CD₃OD) δ 7.32 (m, 5H); 4.79 (dd, J=9.9, 4.4 Hz, 1H); 4.48 (t, J=5.9 Hz, 1H); 3.61 (dd, J=13.9, 6.1 Hz, 1H); 3.51 (dd, J=13.9, 5.7 Hz, 1H); 3.35 (dd, J=14.2, 3.4 Hz, 1H); 3.08 (dd, J=14.2, 9.9 Hz, 1H);
¹³C NMR (62.5 MHz, CD₃OD) δ 175.1, 167.2, 138.2 130.3, 129.7, 128.1, 68.2, 56.2, 51.7, 41.3, 37.5;
MS (ESI) m/z 252 [M+H]⁺, 269 [M+H₂O]⁺.
HRMS calculated for C₁₂H₁₈N₃O₃(M+H) 252.1348; found: 252.1349.
[α]_D ²⁶−38.0 (c 1.9, H₂O)

EXAMPLE 23

L-DAPA-L-Val.2HCl

¹H NMR (300 MHz, D₂O) δ 4.54 (t, J=5.9 Hz, 1H) ; 4.42 (d, J=5.0 Hz, 1H); 3.60 (d, J=5.9 Hz, 2H); 2.29 (m, 1H); 0.97 (t, J=6.7 Hz, 6H);
¹³C NMR (62.5 MHz, D₂O) δ 175.5, 166.9, 59.5, 51.1, 40.3, 30.4, 19.0, 17.6;
MS (ESI) m/z 204 [M+H]⁺;
HRMS calculated for C₈H₁₈N₃O₃: 204.1348; found 204.1365.
[α]_D ²⁶+22.2 (c 2.0, H₂O)

EXAMPLE 24

L-DAPA-D-DAPA.3HCl

¹H NMR (300 MHz, D₂O) δ 4.60 (m, 1H); 4.55 (dd, J=6.5, 4.9 Hz, 1H); 3.65 (m, 3H); 3.45 (dd, J=13.5, 7.7 Hz, 1H);
¹³C NMR (62.5 MHz, D₂O) δ 171.6, 167.6, 51.8, 51.5, 40.2, 40.1;
MS (ESI) m/z 191 [M+H]⁺;
HRMS calculated for C₆H₁₅N₄O₃: 191.1144; found: 191.1146.
[α]_D ²⁶−43.4 (c.0.4, H₂O)

EXAMPLE 28

(2S,3R)-DASA-1-Gly-4-Gly.2HCl

¹H NMR (300 MHz, D₂O) δ 4.61 (s, 1H); 4.43 (s, 1H); 4.06, 4.04 (2s, 4H); ¹³C NMR (62.5 MHz, D₂O) δ 172.9, 172.7, 52.7, 42.5;
MS (ESI) m/z 263 [M+H]⁺; 285 [M+Na]⁺;
HRMS calculated for C₈H₁₅N₄O₆: 263.0992; found: 263.0970.
[α]_D ²⁶−2.2 (c 1.5, H₂O)

EXAMPLE 29

(2S,3S)-DASA-1-L-Val-4-L-Val.2HCl

¹H NMR (300 MHz, D₂O) δ 4.78 (m, 1H); 4.65 (m, 1H); 4.35 (m, 1H), 4.21 (m, 1H); 2.12 (m, 2H); 0.85 (m, 12H);
¹³C NMR (62.5 MHz, D₂O) 175.7, 174.8, 59.2, 59.0, 52.8, 52.3, 30.0, 29.7, 18.4, 18.3, 17.2, 16.9.
MS (ESI) m/z 347 [M+H]⁺, 369 [M+Na]⁺;
HRMS calculated for C₁₄H₂₆N₄O₆Na: 369.1750; found: 369.1760.
[α]_D ²⁶−38.8 (c 0.5, H₂O)

EXAMPLE 30

(2S,3S)-DASA-1-L-Ile-4-L-Ile.2HCl

¹H NMR (300 MHz, D₂O) δ 4.78 (m, 1H); 4.62 (m, 1H); 4.40 (m, 2H); 1.75 (m, 6H); 0.85 (m, 12H);
¹³C NMR (62.5 MHz, D₂O) 173.9, 173.1, 164.9, 163.7, 56.3, 54.8, 52.0, 51.8, 39.4, 24.5, 24.4, 22.5, 22.2, 20.5;
MS (ESI) m/z 375 [M+H]⁺, 397 [M+Na]⁺;
HRMS calculated for C₁₆H₃₀N₄O₆Na: 397.2063; found: 397.1995.
[α]_D ²⁶−18.7 (c 0.3, MeOH)

EXAMPLE 31

L-DAPA-L-Pro.2HCl

¹H NMR (300 MHz, D₂O) δ 4.62 (m, 1H); 4.39 (m, 1H); 3.62 (d, J=7.6 Hz, 1H); 3.57 (m, 2H); 3.42 (dd, J=13.6, 6.1 Hz, 1H); 2.05 (m, 2H); 1.95 (m, 2H);
¹³C NMR (62.5 Hz, D₂O) δ 173.1, 164.9, 59.6, 52.6, 46.0, 39.2, 28.2, 22.3;
MS (ESI) m/z 202 [M+H];
HRMS calculated for C₈H₁₆N₃O₃: 202.1192; found 202.1196.
[α]_D ²⁶−72.2 (c 1.3, H₂O)

EXAMPLE 34

L-DAPA-L-Ala-L-Ala.2HCl

EXAMPLE 35

L-DAPA-L-Ala-L-Val.2HCl

EXAMPLE 36

L-DAPA-L-Ala-L-Pro.2HCl

EXAMPLE 37

D-DAPA-Gly.2HCl

¹H NMR (300 MHz, D₂O) δ 4.42 (t, J=5.8 Hz, 1H), 4.00 (d, J=18.1 Hz, 1H), 4.10 (d, J=18.1 Hz, 1H), 3.53 (d, J=5.8 Hz, 2H);
¹³C NMR (75 MHz, D₂O) δ 172.7, 166.4, 50.5, 41.4, 39.5;
MS (ESI) m/z 162 [M+H]⁺;
HRMS calculated for C₅H₁₂N₃O₃(M+H) 162.0879; found: 162.0863.
[α]_D ²⁵−28.8 (c 1.3, H₂O)

EXAMPLE 38

L-DAPA-GlyOMe.2THF

¹H NMR (300 MHz, CD₃OD) δ 4.39 (t, J=5.7 Hz, 1H), 4.15 (d, J=17.8 Hz, 1H), 4.05 (d, J=17.8 Hz, 1H), 3.75 (s, 3H), 3.52 (dd, J=5.7, 1.5 Hz, 2H);
¹³C NMR (75 MHz, CD₃OD) δ 171.9, 167.5, 53.1, 51.9, 42.1, 41.0;
MS (ESI) m/z 176 [M+H]⁺;
HRMS calculated for C₆H₁₄N₃O₃(M+H) 176.1035; found: 176.1005.
[α]_D ²⁵+24.0 (c 1.1, H₂O)

EXAMPLE 39

L-DAPA-GlyNH₂.2HCl

¹H NMR (300 MHz, D₂O) δ 4.43 (t, J=5.9 Hz, 1H), 4.0 (m, 2H), 3.52 (d, J=5.9 Hz, 2H);
¹³C NMR (75 MHz, D₂O) δ 173.1, 166.7, 50.6, 42.1, 39.4;
MS (ESI) m/z 161 [M+H]⁺;
HRMS calculated for C₅H₁₃N₄O₂(M+H) 161.1039; found: 161.1042.
[α]_D ²⁵+38.6 (c 0.23, H₂O)

EXAMPLE 40

D-DAPA-D-Asp.2HCl

¹H NMR (300 MHz, D₂O) δ 4.75 (m, 1H), 4.38 (dd, J=6.3, 5.3 Hz, 1H), 3.50 (dd, J=14.4, 5.3 Hz, 1H), 3.42 (dd, J=14.4, 6.4 Hz, 1H), 2.93 (d, J=6.3 Hz, 2H);
¹³C NMR (75 MHz, D₂O) δ 174.2, 173.2, 165.9, 50.6, 49.4, 39.4, 35.1;
MS (ESI) m/z 220 [M+H]⁺;
HRMS calculated for C₇H₁₄N₃O₅(M+H) 220.0933; found: 220.0903.
[α]_D ²⁵−32.4 (c 0.7, H₂O)

EXAMPLE 41

D-DAPA-L-Phe.2HCl

¹H NMR (300 MHz, D₂O) δ 7.25 (m, 5H), 4.72 (dd, J=8.7, 5.6 Hz, 1H), 4.28 (dd, J=6.1, 5.7 Hz, 1H), 3.49 (dd, J=14.3, 6.2 Hz, 1H), 3.43 (dd, J=14.3, 5.7 Hz, 1H), 3.22 (dd, J=14.3, 5.6 Hz, 1H), 3.03 (dd, J=14.3, 8.7 Hz, 1H);
¹³C NMR (75 MHz, D₂O) δ 174.4, 165.8, 136.3, 129.2, 128.8, 127.3, 66.6, 54.6, 50.4, 39.6;
MS (ESI) m/z 252 [M+H]⁺
HRMS calculated for C₁₂H₁₈N₃O₃(M+H) 252.1348; found: 252.1357.
[α]_D ²⁵−42.3 (c 1.0, H₂O)

EXAMPLE 42

D-DAPA-D-Phg.2HCl

¹H NMR (300 MHz, D₂O) δ 7.32 (s, 5H), 5.43 (s, 1H), 4.38 (m, 1H), 3.52 (d, J=5.9 Hz, 2H);
¹³C NMR (75 MHz, D₂O) δ 173.2, 165.5, 134.4, 129.3, 127.8, 57.6, 50.4, 39.6;
MS (ESI) m/z 238 [M+H]⁺;
HRMS calculated for C₁₁H₁₁₆N₃O₃(M+H) 238.1192; found: 238.1190.
[α]_D ²⁵−81.4 (c 1.0, H₂O)

EXAMPLE 43

L-DAPA-L-Nle.2HCl

¹H NMR (300 MHz, D₂O) δ 4.35 (m, 2H), 3.48 (d, J=5.9 Hz, 2H), 1.70 (m, 1H), 1.60 (m, 1H), 1.20 (m, 4H), 0.72 (t, J=7.2 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 175.6, 166.1, 57.1, 53.6, 50.4, 39.6, 29.9, 27.0, 21.5, 13.0;
MS (ESI) m/z 218 [M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H) 218.1505; found: 218.1518.
[α]D²⁵+6.8 (c 0.5, H₂O)

EXAMPLE 44

D-DAPA-D-L-Nle.2HCl

EXAMPLE 45

D-DAPA-L-Lys.3HCl

¹H NMR (300 MHz, D₂O) δ 4.42 (dd, J=6.6, 5.1 Hz, 1H), 4.28 (dd, J=8.0, 5.8 Hz, 1H), 3.52 (m, 2H), 2.90 (t, J=7.6 Hz, 2H), 1.82 (m, 2H), 1.60 (m, 2H), 1.37 (m, 2H);
¹³C NMR (75 MHz, D₂O) δ 174.9, 166.1, 53.5, 50.7, 39.5, 39.2, 29.7, 26.3, 22.1;
MS (ESI) m/z 233 [M+H]⁺;
HRMS calculated for C₉H₁₀N₄O₃(M+H) 233.1614; found 233.1624.
[α]_D ²⁵−41.0 (c 0.6, H₂O)

EXAMPLE 46

D-DAPA-L-Leu.2HCl

¹H NMR (300 MHz, D₂O) δ 4.37 (dd, J=6.8, 4.9 Hz, 1H), 4.28 (dd, J=8.0, 6.5 Hz, 1H), 3.49 (m, 2H), 1.7-1.5 (m, 3H), 0.85 (d, J=6.2 Hz, 3H), 0.81 (d, J=6.2 Hz, 3H);
¹³C NMR (75 MHz, D₂O) δ 176.0, 166.0, 52.4, 50.7, 39.6, 39.1, 24.5, 22.0, 20.9;
MS (ESI) m/z 218 [M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H) 218.1505; found: 218.1506.
[α]_D ²⁵−2.5 (c 1.4, H₂O)

EXAMPLE 47

L-DAPA-(CH₂)-L-Val.2TFA

¹H NMR (300 MHz, CD₃OD) δ 3.74 (quintet, J=6.2 Hz, 1H), 3.42 (d, J=4.3 Hz, 1H), 3.36 (dd, J=6.2 Hz, 2H), 3.12-3.10 (m, 2H), 2.25-2.14 (m, 1H), 1.05 (d, J=7.0 Hz, 1H);
¹³C NMR (62.5 MHz, CD₃OD) δ 175.9, 68.3, 49.7, 49.1, 41.6, 32.1, 18.9, 18.8;
MS (ESI) m/z 190 [M+H]⁺;
HRMS calculated for C₈H₁₉N₃O₂(M+H) 190.1556; found: 190.1552.
[α]_D ²⁶+3.0 (c 1.0, MeOH)

EXAMPLE 48

L-DAPA-L-Asp.2TFA

¹H NMR (300 MHz, CD₃OD) δ 4.86 (dd, J=6.3, 4.8 Hz, 1H), 4.42 (t, J=5.7 Hz, 1H), 3.57 (dd, J=13.9, 5.8 Hz, 1H), 3.52 (dd, J=13.9, 5.8 Hz, 1H), 2.99 (dd, J=17.2, 6.2 Hz, 1H), 2.91 (dd, J=17.2, 4.6 Hz, 1H);
¹³C NMR (62.5 MHz, D₂O) δ 174.5, 173.8, 167.1, 51.8, 50.8, 41.2, 36.2;
MS (ESI) m/z 220 [M+H]⁺;
HRMS calculated for C₇H₁₄N₃O₅(M+H) 220.0933; found: 220.0950.
[α]_D ²⁶+36.6 (c 1.0, MeOH)

EXAMPLE 49

D-DAPA-L-(4-trifluoromethyle)-Phe-OH.2HCl

¹H NMR (300 MHz, D₂O) δ 7.58 (d, J=7.9 Hz, 2H), 7.35 (d, J=8.1 Hz, 2H), 4.69 (m, 1H), 4.25 (t, J=6.0 Hz, 1H), 3.27 (dd, J=13.9, 5.8 Hz, 1H), 3.17 (d, J=6.0 Hz, 2H), 3.04 (dd, J=14.0, 9.3 Hz, 1H);
¹³C NMR (75 MHz, D₂O) δ 174.1, 165.7, 140.7, 130.0, 129.6 (q, J=32.9 Hz), 125.5, 125.0 (q, J=271.1 Hz), 54.3, 50.7, 39.5, 36.3;
MS (ESI) m/z 320 [M+H]⁺;
HRMS calculated for C₁₃H₁₇F₃N₃O₃(M+H) 320.1222; found: 320.1236.

EXAMPLE 50

D-DAPA-L-c-trifluoromethyle-Nle-OH.2HCl

EXAMPLE 51

D-DAPA-L-Nle.2HCl

¹H NMR (300 MHz, D₂O) δ 4.43 (dd, J=6.6, 5.1 Hz, 1H), 4.33 (dd, J=8.1, 5.7 Hz, 1H), 3.60 (dd, J=14.5, 5.1 Hz, 1H), 3.53 (dd, J=14.5, 6.6 Hz, 1H), 1.82 (m, 2H), 1.34 (m, 4H), 0.86 (t, J=7.2 Hz, -3H);
¹³C NMR (75 MHz, D₂O) δ 175.6, 166.0, 53.8, 50.6, 39.4, 29.9, 27.1, 21.6, 13.0;
MS (ESI) m/z 218 [M+H]⁺;
HRMS calculated for C₉H₂₀N₃O₃(M+H) 218.1505; found: 218.1506.
[α]_D ²⁵−43.0 (c 1.4, H₂O)

EXAMPLE 52

D-DAPA-DL-p-fluoroPhe.2HCl

¹H NMR (300 MHz, D₂O) δ 7.20-7.15 (m, 2H), 7.03-6.95 (m, 2H), 4.66-4.59 (m, 1H), 4.27 (t, J=5.7 Hz, 0.5H), 4.25 (t, J=5.8 Hz, 0.5H), 3.43 (d, J=6.2 Hz, 0.5H), 3.42 (d, J=5.7 Hz, 0.5H), 3.20-3.10 (m, 1H), 3.17 (d, J=6.0 Hz, 1H), 2.97 (dd, J=14.1, 8.6 Hz, 0.5H), 2.94 (dd, J=14.1, 9.2 Hz, 0.5H);
¹³C NMR (75 MHz, D₂O) δ 174.3, 174.2, 165.8, 165.7, 162.8 (d, J=243.7 Hz), 133.0, 132.8, 131.7, 131.6, 116.3, 116.0, 54.7, 54.5, 50.7, 50.4, 39.5, 35.7, 35.5;
MS (ESI) m/z 270 [M+H]⁺;
HRMS calculated for C₁₂H₁₇FN₃O₃(M+H) 270.1254; found: 270.1255.

EXAMPLE 53

Biochemical and Biological Results

The effectiveness of the novel compounds according to the present invention is shown in the following manner:
Modification of Insulin by Methylglyoxal and the Inhibiting Effect of the Compounds According to the Present Invention: Comparison Between these Products and the Inhibitors of the Prior Art
Human insulin (Ins) is incubated with methylglyoxal (MG) under physiological conditions. After 24 hours, the insulin is completely modified, as illustrated in FIG. 1 (Ins+MG).
On the other hand, insulin is incubated with methylglyoxal in the presence of an equimolar quantity of the AGE inhibitors according to the present invention under physiological conditions. After 24 hours, the modification of insulin by MG is considerably reduced, as is illustrated in FIG. 1.
FIG. 2 illustrates the effectiveness of certain known reactive dicarbonyl scavengers in inhibiting the modification of insulin by MG.
Analyses by HPLC clearly demonstrate that some of the AGE inhibitors according to the present invention scavenge methylglyoxal, which can otherwise modify insulin (FIG. 3). Certain examples of the effects of the AGE inhibitors according to the present invention on somatostatin-14 (containing 2 Lys) and ribonuclease (RNase) A (containing 10 Lys and 4 Arg) are illustrated in FIGS. 4 and 5, respectively.

Electrophoresis Studies of the Inhibiting Capacities of the Novel Compounds According to the Present Invention, MG Scavengers, Against AGE Formation

Ribonuclease A and lysozyme (10 mg/ml) are incubated in the presence of methylglyoxal (10 mM) or in the presence of methylglyoxal and one of the inhibitors according to the present invention in an equimolar quantity at 37° C. After 48 hours of incubation, the proteins are analyzed by polyacrilamide gel electrophoresis (8%-16% SDS PAGE gel).
Analysis of the results shows that in the presence of methylglyoxal, ribonuclease A and lysozyme exhibit extensive modification, which is indicated by the appearance of the dimer form of the protein.
The addition of one of the inhibitors according to the present invention, namely L-DAPA-L-Leu (example 1), L-DAPA-L-Ile (example 18), L-DAPA-L-Val (example 23), D-DAPA-D-Ala (example 8), (2S,3S)-DASA-L-Leu (example 29), L-DAPA-L-Gly (example 17) or L-DABA-L-Leu (example 12), provides protection from these structural modifications caused by methylglyoxal. The presence of inhibitors according to the present invention largely prevents the formation of cross-linked proteins by scavenging methylglyoxal.
The enzymatic activity of ribonuclease A after treatment with methylglyoxal and the various inhibitors according to the present invention is measured using the methylene blue RNA staining technique of Greiner-Stöffele et al. (Anal. Biochem. (1996) 240, 24).
The results are summarized in table 1 below.

	TABLE 1

		Enzymatic
	Condition	activity (%)

	Control RNase	100
	RNase + methylglyoxal	10.11%
	MG + D-DAPA	20.32%
	MG (+) AG	70.52%
	MG + L-DABA-L-Leu (example 12)	80.97%
	MG + D-DAPA-D-Ala (example 8)	87.37
	MG + L-DAPA-L-Gly (example 17)	85.61%
	MG + L-DAPA-L-Phe (example 21)	70.20%
	MG + (2S,3S)-DASA-L-Val (example 29)	75.09%
	MG + L-DAPA-L-(S)-Leu (example 16)	75.69%
	MG + L-DAPA-L-Leu (example 1)	93.91%
	MG + L-DAPA-L-Ile (example 18)	90.77%
	MG + L-DAPA-L-Val (example 23)	90.77%

Enzyme kinetics as measured by spectrophotometry at 688 nm show that the inhibition of enzymatic activity caused by methylglyoxal is considerably reduced in the presence of the inhibitors according to the present invention.
Comparative analyses (by electrophoresis and by enzyme activity measurements) are carried out on ribonuclease A or lysozyme using aminoguanidine (AG) as the inhibitor. The results clearly show that the inhibitors according to the present invention are considerably more effective than AG.
The same trend is observed for lysozyme (containing 6 Lys and 11 Arg) during tests carried out under the same conditions as for ribonuclease A.
MG reacts with a protein,s lysine and arginine residues, thus altering the charges on the modified polypeptide. This was demonstrated by the electrophoresis of glyoxalase I treated with MG under non-denaturing conditions. The exposure of glyoxalase I to MG (10 mM) for 24 hours increases the mobility of the protein toward the positive electrode, a change that is consistent with the loss of positive charges from the ε-amino and guanidino groups and the gain of negative charges. When the inhibitors according to the present invention (L-DAPA-L-Leu (example 1) or L-DAPA-L-Ile (example 18)) are included in the incubation mixture, the presence of these compounds inhibits the gain of negative charge.
The incubation of glyoxalase I, a key protein in the α-oxoaldehyde detoxification system, in the presence of methylglyoxal, modifies the protein. This modification causes a change in charge and a 50% decrease in enzymatic activity compared to the control.
The addition of the compounds according to the present invention (L-DAPA-L-Leu or L-DAPA-L-Ile) prevents the inhibition exerted by methylglyoxal and protects against structural modifications.
Comparative results obtained by electrophoresis show that aminoguanidine (AG) is much less effective than AGE inhibitors (according to the present invention) with respect to the protecting effect of these compounds against the structural modifications of ribonuclease A induced by MG. The AGE inhibitors according to the present invention, namely L-DAPA-L-Leu (example 1) and L-DAPA-L-Ile (example 18), or AG (10 mM) are incubated with MG and ribonuclease A for 40 hours at 37° C.
Growth of EA Cells in the Presence of MG Scavengers According to the Present Invention and of the Prior Art and/or Methylglyoxal
The cells used for the test are from the EA.hy 926 cell line, which are endothelial cells obtained by the hybridization of human umbilical vein endothelial cells (HUVECs) with lung cancer cells (A549). The EA.hy 926 endothelial cells are incubated in Dulbecco's modified Eagle's Medium (DMEM) enriched with 10% fetal calf serum. The cells are incubated in 12-well plates. Each well initially contains 100,000 cells. Cell growth is achieved by incubating the cells in 2 ml of culture medium after adding or not adding the various potential inhibitors (1 mM) and/or methylglyoxal (600 μM) for 48 hours at 37° C. in a moist atmosphere with 5% CO₂.
The number of cells is evaluated in the following way:
The cells are stained using the (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MTT penetrates in the cell where it is converted into formazan. The quantity of formazan formed is proportional to the number of living cells.
The results are expressed as a relative percentage of the number of cells after treatment compared to the number of control cells without treatment [100*OD (treated cells)/OD(control cells)]. Detection is carried out by UV/visible spectrophotometry at 570 nm.
Principle: MTT (yellow) penetrates the cell and is converted into an insoluble blue compound, formazan, by cleavage of its tetrazolium rings by the mitochondrial dehydrogenase enzymes of living cells. Formazan is solubilized by isopropanol. The number of cells is proportional to the quantity of formazan formed and its absorbance.
As illustrated in FIG. 6, methylglyoxal (MG) suppresses cell growth.
The results are summarized in table 2 below.

TABLE 2

Cell growth in the presence or absence of MG
and MG scavengers

			Cells +
	Cells +		MG
	MG		scavengers +
	scavengers		Methylglyoxal		Difference
MG scavengers	(A)	SD	(B)	SD	(B) − (A)

0	100	0	24
Aminoguanidine	97	6	92	5	−5
L-DAPA-L-	94	8	93	3	−1
Val•2HCl
(example 23)
L-DAPA-L-	94	16	81	7	−13
Leu•2HCl
(example 1)
Carnosine	99	0.2	50	3	−49
L-DAPA-L-	86	6	79	4	−7
Ile•2HCl
(example 18)
(2S,3S)-DASA-L-	85	5	70	9	−15
Val•2HCl
(example 29)
L-DABA-L-	77	15	73	9	−4
leu•2HCl
(example 12)
L-DAPA-L-	77	6	71	7	−6
Phe•2HCl
(example 21)
Metformin	91	4	45	4	−46
D-DAPA	83	3	68	13	−15
D-DAPA-D-	77	6	71	7	−6
Ala•2HCl
(example 8)
L-DAPA-L-(S)-	71		74		+3
Leu•2HCl
(example 16)
L-DAPA-Gly•2HCl	54	8	50	10	−4
(example 17)
(2S,3R)-DASA-	61	8	54	11	−7
Gly•2HCl
(example 28)
L-Lys	81		43		−38

Mean of 3 experiments

The addition of aminoguanidine (AG), a known MG scavenger, suppresses this process in a spectacular manner. The same trend can be observed with the compounds according to the present invention, in particular L-DAPA-L-Val (example 23), L-DAPA-L-Leu (example 1) and L-DAPA-L-Ile (example 18). Other known MG scavengers, such as carnosine and metformin, proved less effective in this test. Additional examples of the inhibiting effect of the compounds according to the present invention compared to the suppression of cell growth by MG are illustrated in FIG. 7.
These results show that the compounds according to the present invention are non-toxic with respect to EA cells. This is true in particular for L-DAPA-L-Val.2HCl (example 23), L-DAPA-L-Leu.2HCl (example 1), L-DAPA-L-Ile.2HCl (example 18), (2S,3S)-DASA-L-Val.2HCl (example 29) and L-DAPA-L-Leu.2TFA (example 3) for which the number of cells is lower by less than 15% compared to the number of control cells growing without the addition of any product. The composition of the molecule's diamino moiety is not involved in toxicity nor is the associated salt. Indeed, L-DAPA, (2S,3S)-DASA and D-DAPA, as well as HCl and TFA salts, are found in toxic and nontoxic products. It can be noted that the non-toxicity of the compounds increases their MG-scavenging activity compared to cells growing with MG alone. The difference between the relative values of the number of cells growing with the analyzed compound and the cells growing in the presence of MG and the analyzed compound makes it possible to evaluate the product's role as a MG scavenger. Eight compounds according to the present invention possess this activity in particular, namely L-DAPA-L-Val.2HCl (−3) (example 23), L-DAPA-L-Leu.2HCl (−13) (example 1), L-DAPA-L-Ile.2HCl (−9) (example 18), (2S,3S)-DASA-L-Val.2HCl (−15) (example 29), L-DAPA-L-Leu.2TFA (−18) (example 3), L-DABA-L-Leu.2HCl (−4) (example 12) and L-DAPA-L-Phe,.2HCl (−6) (example 21). Two other compounds also exhibit scavenging activity, namely D-DAPA-D-Ala.2HCl (+1) (example 8) and L-DAPA-Gly.2HCl (+3) (example 17). On the other hand, their cell toxicity is higher (396 and 43%, respectively). It can be noted that metformin is a weak scavenger even though this molecule has extremely low toxicity at this concentration.

Test of Mutagenicity of Two Compounds According to the Present Invention: L-DAPA-L-Leu (Example 1) and L-DAPA-L-Val (Example 23)

An Ames test was performed with L-DAPA-L-Leu (example 1) and L-DAPA-L-Val (example 23) alone and in combination with methylglyoxal on human liver S9 fractions and on seven strains of Salmonella.
The concentrations used in the Ames test were as follows:

- L-DAPA-L-Leu alone or L-DAPA-L-Val alone: 10 μM, 1 μM and 0.1 μM.
- L-DAPA-L-Leu/methylglyoxal mixture or L-DAPA-L-Val/methylglyoxal mixture: 10 μM, 1 μM and 0.1 μM.
- Methylglyoxal: 10 μM.

The results are summarized in table 3 below.

TABLE 3

	Concentration	Human	Six mixed	Strain
Substance tested	(μM)	S9	strains	TA98

L-DAPA-L-Leu	10/1/0.1	No	−	−
(example 1)
L-DAPA-L-Val	10/1/0.1	No	−	−
(example 23)
L-DAPA-L-Leu	10/1/0.1	Yes	−	−
example 1:
(metabolites)
L-DAPA-L-Val	10/1/0.1	Yes	−	−
example 23:
(metabolites)
L-DAPA-L-Leu	10/1/0.1	No	−	−
(example 1) +
methylglyoxal
L-DAPA-L-Val	10/1/0.1	No	−	−
(example 23) +
methylglyoxal
L-DAPA-L-Leu	10/1/0.1	Yes	−	−
(example 1) +
methylglyoxal
(metabolites)
L-DAPA-L-Val	10/1/0.1	Yes	−	−
(example 23) +
methylglyoxal
(metabolites)
Methylglyoxal	10	No	−	−
Methylglyoxal	10	Yes	−	−
(metabolites)
Positive control	2.6/9.5	No	+	+
(4NQO)/2NF)
Positive control	51.7	Yes	+	+
(2AA) (metabolites)
Negative control	—	No	−	−
(solvent)
Negative control	—	Yes	−	−
(solvent)

−: non-mutagenic
+: mutagenic

Tested alone or in combination with methylglyoxal, neither substance (L-DAPA-L-Leu or L-DAPA-L-Val) was mutagenic for TA98, the mixed strains or the human liver S9 fractions at the concentrations tested. The metabolites produced by the human liver S9 fractions were not mutagenic at the concentrations tested.

Claims

1. A compound of the following general formula I:

wherein:

X represents CH₂, C═O, C═S or CHOH, R₁represents an amino acid, optionally substituted by one or more halogen atoms, or by one or more CF₃groups, and n=0, 1 or 2

or X represents CH₂, C═O, C═S or CHOH, R₁represents a peptide containing two amino acids, each amino acid being optionally substituted by one or more halogen atoms, or one or more CF₃groups, and n=0 or 1 or XR₁represents PO₃H or SO₃H and n=0, 1 or 2;

R₂represents H, XR₁, a C₁-C₆alkyl group, a C₁-C₆aralkyl group or an aryl group, the alkyl, aralkyl and aryl groups being able to be substituted by NH₂, a carboxylic group (COOH), one or more halogen atoms, or one or more CF₃groups;

or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same,

with the exception of compounds

wherein R₂represents a hydrogen atom, X represents C═O, R₁represents —NH—(CH₂)_m—COOH and m=1, 2 or 3 and n=0, 1 or 2;

represented by the following formulas:

and the compounds L-ornithyl-taurine, L-diaminobutyryl-taurine and L-diaminopropionyl taurine.

2. A compound according to claim 1, wherein X represents C═O, CH₂or C═S.

3. A compound according to claim 1, wherein R₂represents XR₁or H.

4. A compound according to claim 1, wherein R₁represents an amino acid and n=0, 1 or 2.

5. A compound according to claim 1, wherein said compound it is represented by the following general formula II:

wherein:

R₁represents —NH—R₃—(C═O) R₄or

R₃represents

a C₁-C₁₂alkyl group, optionally substituted by one or more groups chosen among a halogen atom, a —CF₃, phenyl, phenol, —COOH, amine or phenyl group substituted by one or more halogen atoms, or by one or more CF₃groups;

a phenyl group, optionally substituted by an amine, an OH group, one or more halogen atoms, or one or more CF₃groups and

R₄represents OH, NH₂, a C₁-C₃₀alkoxy,

R₂represents H, COR₁, or a C₁-C₆alkyl group optionally substituted by one or more halogen atoms, or by one or more CF₃groups;

n=0, 1 or 2;

Y represents an oxygen or sulfur atom;

or the pharmaceutically acceptable addition salts, isomers, enantiomers and diastereoisomers of same, as well as mixtures of same.

6. A compound according to claim 1 or 5, wherein n=0.

7. A compound according to claim 1, wherein said compound is selected among

8. A method for preventing the deterioration of proteins in foods comprising the addition to the food of a compound according to claim 1 or 5.

9. A pharmaceutical or cosmetic composition comprising a compound according to claim, 1 or 5 and a pharmaceutically or cosmetically acceptable excipient.

10. (canceled)

11. A method according to claim 19 or 20, wherein said compound is represented by the following general formula II:

wherein:

R₁represents NH—R₃—(C═O) R₄or

wherein

R₃represents

R₄represents OH, NH₂, a C₁-C₃₀alkoxy;

n=0, 1 or 2;

Y represents an oxygen or sulfur atom;

12. A method according to claim 19 or 20, wherein said compound is selected among

13-15. (canceled)

16. A method according to claim 19, wherein the compound is administered by oral route.

17. (canceled)

18. A compound according to claim 4 wherein the amino acid R₁is selected among alanine, valine, isoleucine, proline, leucine, norleucine, phenylalanine or tert-leucine.

19. A method for scavenging reactive carbonyl compounds, for inhibiting the formation of advanced glycation end-products, for the prevention and/or the treatment of a state or disease due to the formation of advanced glycation end-products or to the cross-linking of proteins, for the prevention and/or the treatment of the deleterious effects of the ageing of an organism, said effects being the formation of advanced glycation end-products or the cross-linking of proteins, or in a patient for the slowing or the stopping of the progression of complications resulting from diabetes, said complications resulting from the formation of advanced glycation end-products or from the cross-linking of proteins, for treating, preventing and/or slowing in a patient the progression of diseases chosen among rheumatoid polyarthritis, Alzheimer's disease, uremia, neurodegenerative diseases, atherosclerosis, microvascular and macrovascular complications of diabetes including diabetic retinopathy and renal failure due to diabetic nephropathy, microangiopathies and macroangiopathies, cataracts, amyloidosis associated with dialysis or with Alzheimer's disease, Parkinson's disease, gingivitis, cavities, bucco-dental conditions, diabetic ulcers, chronic renal failure, chronic renal dialysis, inflammatory diseases, age-related rheumatic disorders and porphyria and for treating early-stage cancers, comprising the administration of an effective amount of a compound of following general formula I:

wherein:

or X represents CH₂, C═O, C═S or CHOH, R₁represents a peptide containing two amino acids, each amino acid being optionally substituted by one or more halogen atoms, or one or more CF₃groups, and n=0 or 1

or XR₁represents PO₃H or SO₃H and n=0, 1 or 2;

R₂represents H, XR₁, a C₁-C₆alkyl group, a C₁-C₆aralkyl group or an aryl group, the alkyl, aralkyl and aryl groups being able to be substituted by a NH₂, a carboxylic group, one or more halogen atoms, or one or more CF₃groups;

with the exception of the compound

to a patient in need thereof.

20. A method for treating the skin to reduce evidence of wrinkles and ageing and to restructure the epidermis and the papillary dermis comprising the administration of an effective amount of a compound of following general formula I:

wherein:

or X represents CH₂, C═O, C═S or CHOH, R, represents a peptide containing two amino acids, each amino acid being optionally substituted by one or more halogen atoms, or one or more CF₃groups, and n=0 or 1

or XR₁represents PO₃H or SO₃H and n=0, 1 or 2;

with the exception of the compound

to a patient in need thereof.