US2186902A - Urine acetone test - Google Patents
Urine acetone test Download PDFInfo
- Publication number
- US2186902A US2186902A US266539A US26653939A US2186902A US 2186902 A US2186902 A US 2186902A US 266539 A US266539 A US 266539A US 26653939 A US26653939 A US 26653939A US 2186902 A US2186902 A US 2186902A
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- US
- United States
- Prior art keywords
- urine
- color
- acetone
- test
- ammonium
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- Expired - Lifetime
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/64—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving ketones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/20—Oxygen containing
- Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing
- Y10T436/202499—Formaldehyde or acetone
Definitions
- My. invention permits an accurate acetone test to be made readily and rapidly, even by unskilled persons, with no other apparatus than a test tube, with no liquid acid or alkali, with little or no chance of material error from the observers personal equation, and with no necessity for keeping two liquid layers separate and 1 for reading a color at a mere interface, because with my test the color is produced through the whole mass of a liquid.
- a physician can make my test in the sick room within three minutes, and know at once from his own observation what a patients condition is with respect to acetone in the urine.
- Acetone is likely to occur in many conditions, such as nephritis and malnutrition, especially post-operative malnutrition, but most particularly and most dangerously in diabetic coma, to mention but a few; and sometimes the condition may be such that the sav-- the urine as the treatment progresses, so that.
- the soluble nitroprusside is most conveniently sodium nitroprus-, side, although other alkali-metal nitroprussides may be used.
- the soluble carbonate is most conveniently sodium carbonate, although other soluble alkali-metal carbonates may be used.
- ammonium salt is most conveniently ammonium
- ammonium salts such for instance as ammonium nitrateor ammonium chloride or ammonium citrate; or
- ammonium carbonate if the ingredients are added directly, although that is not suitable if theingredients are to be kept for some time before use, because of the instability-of ammonium carbonate.
- The'material which will cause the observed color to be that due to reflected'light is some finely divided insoluble material, such for instance as finely ground talc or marble; desirably with some protective colloid also present,
- gelatin capsule which may be carried in the action unit is desirable for Conventional Mg. acetone per Color designation 100 cc. of urine 1 Light-greenishyellow Faint trace 15-25. 2 Flesh Trace 40-60. 3 Light violet l. 1+ -80. 4 Medium violet- 2+ -110. 5 Deep violet 3+ -150. 6 Dark purple-blue l 4+ Greater than 150.
- the physician 1 l Takes 4 cc. of the patients urine, by fillin the test tube to the first mark.
- the dilution of the urine before adding the reseveral reasons prevents the precipitation, or causes the re-solution, of salts such as calcium or magnesium carbonates, which may be present in the urine.
- the color produced corresponds rather closely to the color produced in the interface test now commonly made, and indeed so closely that a technician experienced in that interface test can often read the color produced by my test with substantial accuracy even without making actual comparisons with the color chart.
- a process for testing for acetone in urine which consists in diluting a fixed quantity of the urine, and adding to the diluted urine a soluble nitroprusside, a soluble carbonate which will produce an alkalinity at least as great as pH 10, an ammonium salt, and a material which will cause the color produced to be that observed by reflected light, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
- a process for testing for acetone in urine which consists in adding to a fixed quantity of urine a soluble nitroprusside, a soluble carbonate which will produce an alkalinity at least as great as pH 10, an ammonium salt, and a material which will cause the color produced to be that observed by reflected light, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
- a process for testing for acetone in urine which consists in diluting to a fixed volume a fixed smaller volume of urine, and adding to the diluted urine a definite quantity of a fixed-proportion mixture containing sodium nitroprusside, a sodium carbonate, ammonium sulfate, talc, and soap, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
- a process for testing for acetone in urine which consists in adding to a fixed quantity of urine a definite quantity of a fixed-proportion mixture containing sodium nitroprusside, sodium carbonate, ammonium sulfate, talc, and soap, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
- a reaction unit which reaction unit contains in solid form a soluble nitroprusside, a soluble carbonate which is capable of producing an alkalinity as great as pH 10, a stable ammonium salt, and a material which when the contents of the reaction unit are put into solution will cause the observed color produced to be that due to reflected light.
- reaction unit which reaction unit contains in solid form sodium nitroprusside, sodium carbonate, ammonium sulfate, talc, and soap.
Description
Patented Jan. 9, 1940 v UNITED s rts- 2,186,902 URINE AcEToNE-TEsr William B. Fortune, Indianapolis, Ind., assignor to Eli Lilly and Company, Indianapolis, Ind., acorporation of Indiana No Drawing. Application April '7, 1939, Serial No. 266,539
's'oiaims. (01. 23-230) It is the object of my invention to provid 'j simple, rapid, and convenient test for acetone in urineyand one that can readily be made by a physician in a sick room without laboratory equipment, and without the need of other liquid than the urine itself and conveniently some water, which may be ordinary tap water.
The usual procedure for testing for acetone in .urine'is one that requires considerable skill and experience, as well as some technical knowledge;
and it also requires the use of at least one reagent that is available only in liquid form. While this:
can be done in hospitals and in clinical laboratories, even there it involves some technical difficulties,'and such a large chance of error due to the personal equation of the technician that l the error is often as high as 50%; and it is practically impossible for the general physician to use in the ordinary home sick room. In that usual procedure for making this acetone test,
a color is produced at the interface between two liquid layers, involving not only skill and a great chance of error in connection with determining the color and what it indicates, but also. very considerable skill in keeping the two liquid layers separate, since even relatively slight mixing of the two layers makes the test inaccurate.
My. invention permits an accurate acetone test to be made readily and rapidly, even by unskilled persons, with no other apparatus than a test tube, with no liquid acid or alkali, with little or no chance of material error from the observers personal equation, and with no necessity for keeping two liquid layers separate and 1 for reading a color at a mere interface, because with my test the color is produced through the whole mass of a liquid. A physician can make my test in the sick room within three minutes, and know at once from his own observation what a patients condition is with respect to acetone in the urine.
Such immediate knowledge is desirable for the physician in many pathological conditions, in order that he may from the beginning conduct his treatment accordingly. Acetone is likely to occur in many conditions, such as nephritis and malnutrition, especially post-operative malnutrition, but most particularly and most dangerously in diabetic coma, to mention but a few; and sometimes the condition may be such that the sav-- the urine as the treatment progresses, so that.
prompt knowledge at any particular point the treatment is oitremendous value.
My acetone test meets the requirements of the physician in this respect.
- Inaccordance with my invention, I proceed; generally as follows:
I take a fixed quantity of urine to be tested, say 4 cc., desirably in a test tube bearing a mark indicating that amount. For reasons which will;
appear, I then desirably dilute the urine to twice its volume, with ordinary tap water if no other is available, as by adding 4 cc. of water if the original amount of urine was 4 cc., conveniently by adding that water to a second mark on the test tube; although this dilution step is not one that is essential to my. invention. ThenI add to the urine,=which has preferably been diluted as stat-ed, a soluble nitroprusside, a soluble carbonate that will give an alkalinity at least as great as pH 10, an ammonium salt, and a material which will cause the observed color to be that due to reflected light. The soluble nitroprusside is most conveniently sodium nitroprus-, side, although other alkali-metal nitroprussides may be used. The soluble carbonate is most conveniently sodium carbonate, although other soluble alkali-metal carbonates may be used. The
ammonium salt is most conveniently ammonium,
sulfate, although other ammonium salts may be used, such for instance as ammonium nitrateor ammonium chloride or ammonium citrate; or
even ammonium carbonate if the ingredients are added directly, although that is not suitable if theingredients are to be kept for some time before use, because of the instability-of ammonium carbonate. The'material which will cause the observed color to be that due to reflected'light is some finely divided insoluble material, such for instance as finely ground talc or marble; desirably with some protective colloid also present,
Sodium nitroprusside Sodium carbonate monohydrate 200-220 mg. Ammonium sulfatel 200-220 mg. Talc 15- 25mg. Soap ,5- 10 mg.
These ingredients are all dry reagents; and.
gelatin capsule, which may be carried in the action unit is desirable for Conventional Mg. acetone per Color designation 100 cc. of urine 1 Light-greenishyellow Faint trace 15-25. 2 Flesh Trace 40-60. 3 Light violet l. 1+ -80. 4 Medium violet- 2+ -110. 5 Deep violet 3+ -150. 6 Dark purple-blue l 4+ Greater than 150.
In making the test, the physician 1 l. Takes 4 cc. of the patients urine, by fillin the test tube to the first mark.
2. Adds 4 cc. of tap water, by adding tap water to the test tube to the second mark.
3. Dumps into the test tube the contents of one capsule containing the ingredients above referred to.
4. Shakes vigorously; and then allows the tube to stand for about two to two and a half minutes.
5. Then promptly compares the color of the test-tube contents with the colors of the color chart, to determine directly by matching the colors the content of acetone in the urine.
The dilution of the urine before adding the reseveral reasons. First, such dilution prevents the precipitation, or causes the re-solution, of salts such as calcium or magnesium carbonates, which may be present in the urine. Second, the dilution spreads the color over a larger range, and facilitates comparison with the color chart; and the color chart I prefer is graduated on the basis of the diluted urine, although one can be made and used which is graduated on the basis of undiluted urine. Third, by diluting the urine, and using the quantities of reagents indicated, the color produced corresponds rather closely to the color produced in the interface test now commonly made, and indeed so closely that a technician experienced in that interface test can often read the color produced by my test with substantial accuracy even without making actual comparisons with the color chart.
I claim as my invention:
1. A process for testing for acetone in urine, which consists in diluting a fixed quantity of the urine, and adding to the diluted urine a soluble nitroprusside, a soluble carbonate which will produce an alkalinity at least as great as pH 10, an ammonium salt, and a material which will cause the color produced to be that observed by reflected light, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
2. A process for testing for acetone in urine, which consists in adding to a fixed quantity of urine a soluble nitroprusside, a soluble carbonate which will produce an alkalinity at least as great as pH 10, an ammonium salt, and a material which will cause the color produced to be that observed by reflected light, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
3. A process for testing for acetone in urine, which consists in diluting to a fixed volume a fixed smaller volume of urine, and adding to the diluted urine a definite quantity of a fixed-proportion mixture containing sodium nitroprusside, a sodium carbonate, ammonium sulfate, talc, and soap, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
4. A process for testing for acetone in urine, which consists in adding to a fixed quantity of urine a definite quantity of a fixed-proportion mixture containing sodium nitroprusside, sodium carbonate, ammonium sulfate, talc, and soap, to produce a color which by comparison with a color chart indicates the quantity of acetone in the urine.
5. A reaction unit, which reaction unit contains in solid form a soluble nitroprusside, a soluble carbonate which is capable of producing an alkalinity as great as pH 10, a stable ammonium salt, and a material which when the contents of the reaction unit are put into solution will cause the observed color produced to be that due to reflected light.
6. A reaction unit, which reaction unit contains in solid form sodium nitroprusside, sodium carbonate, ammonium sulfate, talc, and soap.
' WILLIAM B. FORTUNE.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US266539A US2186902A (en) | 1939-04-07 | 1939-04-07 | Urine acetone test |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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US266539A US2186902A (en) | 1939-04-07 | 1939-04-07 | Urine acetone test |
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US2186902A true US2186902A (en) | 1940-01-09 |
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US266539A Expired - Lifetime US2186902A (en) | 1939-04-07 | 1939-04-07 | Urine acetone test |
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Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2509140A (en) * | 1948-03-02 | 1950-05-23 | Miles Lab | Test reagent composition |
US2564247A (en) * | 1947-08-18 | 1951-08-14 | Richard M Carson | Method of testing urine to determine pregnancy |
US2577978A (en) * | 1949-02-04 | 1951-12-11 | Miles Lab | Diagnostic composition |
US2828665A (en) * | 1954-05-25 | 1958-04-01 | Umezu Motoyosi | Ketosis diagnosing instruments equipped with built-in colorimeter |
US2891438A (en) * | 1950-03-23 | 1959-06-23 | S L F Engineering Company | Photoelectric photometer having compensating means for line voltage fluctuations |
US3068071A (en) * | 1958-05-14 | 1962-12-11 | Roussel Uclaf | Determination of urea |
DE1153920B (en) * | 1957-10-21 | 1963-09-05 | Miles Lab | Diagnostic agent for the determination of ketone compounds in body fluids |
US3212855A (en) * | 1962-08-06 | 1965-10-19 | Miles Lab | Diagnostic device |
US3880590A (en) * | 1973-11-08 | 1975-04-29 | Shionogi & Co | Test strip for ketone bodies |
DE2838675A1 (en) * | 1977-11-21 | 1979-05-23 | Miles Lab | TEST MEANS AND METHOD FOR DETECTION OF KETONE BODIES |
US4193766A (en) * | 1978-11-13 | 1980-03-18 | Miles Laboratories, Inc. | Device and method for preparation of a control solution for ketone determination |
US4931404A (en) * | 1986-12-22 | 1990-06-05 | Abbott Laboratories | Method and device for ketone measurement |
US4970172A (en) * | 1986-12-22 | 1990-11-13 | Abbott Laboratories | Method and device for ketone measurements |
US5071769A (en) * | 1986-12-22 | 1991-12-10 | Abbott Laboratories | Method and device for ketone measurement |
CN105445267A (en) * | 2015-12-30 | 2016-03-30 | 海南赛维埃克斯生物科技有限公司 | Sensor and device for detection of acetone during breathing, and production method of device |
US9518991B2 (en) | 2012-05-15 | 2016-12-13 | Invoy Technologies, Llc | Method and apparatus for analyzing acetone in breath |
-
1939
- 1939-04-07 US US266539A patent/US2186902A/en not_active Expired - Lifetime
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2564247A (en) * | 1947-08-18 | 1951-08-14 | Richard M Carson | Method of testing urine to determine pregnancy |
US2509140A (en) * | 1948-03-02 | 1950-05-23 | Miles Lab | Test reagent composition |
US2577978A (en) * | 1949-02-04 | 1951-12-11 | Miles Lab | Diagnostic composition |
US2891438A (en) * | 1950-03-23 | 1959-06-23 | S L F Engineering Company | Photoelectric photometer having compensating means for line voltage fluctuations |
US2828665A (en) * | 1954-05-25 | 1958-04-01 | Umezu Motoyosi | Ketosis diagnosing instruments equipped with built-in colorimeter |
DE1153920B (en) * | 1957-10-21 | 1963-09-05 | Miles Lab | Diagnostic agent for the determination of ketone compounds in body fluids |
US3068071A (en) * | 1958-05-14 | 1962-12-11 | Roussel Uclaf | Determination of urea |
US3212855A (en) * | 1962-08-06 | 1965-10-19 | Miles Lab | Diagnostic device |
US3880590A (en) * | 1973-11-08 | 1975-04-29 | Shionogi & Co | Test strip for ketone bodies |
DE2838675A1 (en) * | 1977-11-21 | 1979-05-23 | Miles Lab | TEST MEANS AND METHOD FOR DETECTION OF KETONE BODIES |
US4193766A (en) * | 1978-11-13 | 1980-03-18 | Miles Laboratories, Inc. | Device and method for preparation of a control solution for ketone determination |
US4931404A (en) * | 1986-12-22 | 1990-06-05 | Abbott Laboratories | Method and device for ketone measurement |
US4970172A (en) * | 1986-12-22 | 1990-11-13 | Abbott Laboratories | Method and device for ketone measurements |
US5071769A (en) * | 1986-12-22 | 1991-12-10 | Abbott Laboratories | Method and device for ketone measurement |
US9518991B2 (en) | 2012-05-15 | 2016-12-13 | Invoy Technologies, Llc | Method and apparatus for analyzing acetone in breath |
US10352940B2 (en) | 2012-05-15 | 2019-07-16 | Invoy Holdings, Llc | Method and apparatus for analyzing acetone in breath |
US11353462B2 (en) | 2012-05-15 | 2022-06-07 | Invoy Holdings Inc. | Method and apparatus for analyzing acetone in breath |
CN105445267A (en) * | 2015-12-30 | 2016-03-30 | 海南赛维埃克斯生物科技有限公司 | Sensor and device for detection of acetone during breathing, and production method of device |
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