US2509140A - Test reagent composition - Google Patents
Test reagent composition Download PDFInfo
- Publication number
- US2509140A US2509140A US12699A US1269948A US2509140A US 2509140 A US2509140 A US 2509140A US 12699 A US12699 A US 12699A US 1269948 A US1269948 A US 1269948A US 2509140 A US2509140 A US 2509140A
- Authority
- US
- United States
- Prior art keywords
- acetone
- urine
- bodies
- diagnostic
- glycine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/64—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving ketones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/20—Oxygen containing
- Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing
Definitions
- the present invention relates to new-and inn proved diagnostiv compositions usefuliorr the.”
- the invention relates todie agnostic compositions. in dry-: form; preferably tabletted 511111) suitable; sized tablets; which com;-
- the medicalqpram titioner is well aware of the usef-ulnessiofxtests-r for acetonebodiesin theurinein diabetes-.; mel-.-.
- Powder or dry reagent preparations-which contain an ammonia yielding ingredient are inherently unstable and-liberate ammonia and change" color .even when stored. in air-tight containe'rs.
- the object of the present invention is theprovision. of diagnostic compositions: in stabledry 'io'rm; preferably tablets, which can be. used even-by an unskilled person conveniently and readily 'to give an accurate qualitative test for, and a quantitative estimation' of," the presence'of 'acetone bodies in .urine, which test clearly distinguishes between posi tive and negative specimens.
- An. important object ofithe invention is the provision. of a "drwdiagnostic composition for the detection of acetonebodies "in urine-whichdoes not evolve ammonia either: in the dry state or "in-'the'wet'state; Certain otherobj'ects of the invention will, in-part,-be-obvious' and-wilt in part appear hereinafter;
- a solublenitro r-usside in. the ros ence of an aliphatic amino acid under alkaline conditions; provides a diagnostid composition which is particularly adapted for the detection of acetone inurine:
- This diagnostic or zreagent composition may beformulate'd in dry form; most suitabl lastablets.
- compositions-isgthe-ifact that: acetone negative specie positions of the present invention should be made within one minute of the time that the powder or tablet is moistened with the specimen under examination.
- the aliphatic amino acid of choice for the diagnostic compositions of the present invention is glycine (i. e. glycocol or amino acetic acid). This compound gives a. high quality coloration and has no blank color.
- Other aliphaticamino acids which give bright colors and which may be used in the diagnostic compositions are: alanine, glutamic acid, arginine, aspartic acid and lysine.
- alkaline conditions are required for testin specimens for acetone bodies in accordance with the invention.
- the a1- kaline material of choice for providing such alkalinity is disodium phosphate.
- With a dry powder or tablet preparation it is possible to use other alkaline solids such as carbonates, hy-
- alkali metal salts of the amino acids such as sodium or potassium glycinate.
- disodium phosphate is the substance of choice since it is readily obtained as an anhydrous salt and its action in the test appears to be superior to that of a number of the other alkaline materials which can be used.
- Any soluble nitroprusside appears to be useful in the reagent composition.
- any one of the alkali metal nitroprussides can be used. How-.
- diluents along with the active constituents of the diagnostic compositions.
- diluents which have been satisfactorily used include starch, lactose, the borates, specifically sodium borate,
- the diagnostic compositions may, if desired, be used in powder or granular form and because of their stability in aqueous solution, the
- compositions may even be used in liquid form.
- the proportions of the amino acid, soluble nitroprusside and alkaline material are not critical within broad limits, and these ingredients.
- this ingredient should not comprise over approximately 5% by weight of the total bulk of the mixture and should represent at least 0.04% of the total bulk thereof.
- the preferred range of concentration is from 0.5% to 1%.
- Other soluble nitroprussides are preferably used in equivalent concentrations.
- the relative proportions of disodium ph-os-' phate and glycine, or their equivalents, in the reagent compositions can vary quite widely and still the reagent will give satisfactory results with both acetone positive and acetone negative urines.
- Example 1 50 grams of disodium phosphate, anhydrous
- the preferred technique for testingspecimens for acetone using the diagnostic compositions of the present invention involves placing a tablet, or an equivalent pile of powder, on a flat surface and moistening the tablet or powder deposite with one drop of the urine specimen under examination.
- acetone-containing urines cause the tablet or powder to acquire a characteristic type of purple or violet color, the intensity or shade of the color being proportional to the concentration of acetone bodies present.
- With negative urine samples the tablet or powder either does not undergo any color change or may assume a pale yellow or brown color which is easily diiferentiated from the characteristic purple color of the positive specimen.
- Diagnostic compositions made in accordance with the present invention have been used clinically to study a series of acetone-containing urines from hospital patients as well as normal urines from healthy individuals. In all instances the urines were examined and checked by previously established methods as well as with the new reagent. The results of these clinical studies clearly showed that the diagnostic compositions of the present invention were particularly suited for use in detecting ketonuria and also for estimating the amount of ketone bodies in the urine. The clinical study emphasized the fact that the reagent formulations of the present invention were very reliable and that there was no difiiculty occasioned due to the confusion of the results of tests with negative urines and with positive urines.
- the diagnostic compositions of the present invention have a number of advantages which increase their Value as reagents for testing specimens for the presence of acetone.
- the combination of disodium phosphate and glycine, or the equivalents thereof suggested above comprises an extremely well buffered system that is unafiected by the reaction of the urine being tested.
- n0 objectionable fumes of ammonia or other noxious gas are evolved as in the case with dry reagent compositions of the prior art. This is an important feature since in many instances, a technician will be testin a large number of specimens at the same time, often in a small room without adequate ventilation.
- the combination of disodium phosphate and glycine, or their equivalents, is not reactive and is, accordingly, very stable.
- the anhydrous sodium phosphate is capable of taking up small amounts of water without deterioration so that the combination remains fully reactive even under adverse conditions of humidity,
- a diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia consisting essentially of, from 0.04-5.0% of sodium nitroprusside, from 1-80% of glycine, and from 20-99% of disodium phosphate.
- a diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia consisting essentially of, approximately 5 parts of glycine, approximately 20 parts of disodium phosphate, and approximately 0.2 of a part of sodium nitroprusside.
- a diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia consisting essentially of, approximately 2.5 parts of glycine, approximately parts of disodium phosphate, approximately 0.8 part of sodium nitroprusside, and approximately 50 parts of sodium borate.
- a diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia consisting essentially of from 0.04-5.0% of water soluble nitroprusside, from 1-80% of an aliphatic amino acid, and from 20-99% of an alkaline material.
- a diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia consisting essentially of from 0.045.0% of an alkali metal nitroprusside, from 1-80% of an aliphatic amino acid, and from 20-99% of an alkaline alkali metal phosphate.
Description
Patented May 23, 1950 Alfred H; "Free. Elkhai-tj 11141., assignor -to--Miles- Laboratories; :Inc., Elkhart, Ind., a. corporation of Indiana;-
No Drawing, ApplicationMarch 2,1948,
Serial No. 12,1699
The present invention relates to new-and inn proved diagnostiv compositions usefuliorr the."
qualitative detection and quantitativeestimation' of: ketonebo'dies in body fluids, particularly:
acetone bodies .inth'e urine.
More specifically, the invention-relates todie agnostic compositions. in dry-: form; preferably tabletted 511111) suitable; sized tablets; which com;-
positions can .readilybeaused even .byr-unskilled:
persons rapidly to .detect' the presence rof -ade'e tone" in .urine. without evolvement of ammonia;.
and without the use ofyequipment or apparatus:
other than some meansrfor'obtalning' a: drop. or-
test fluid:v
droxy-butyric acid. Under normal circumstances no significant quantity of these ketone. substances appears in the urine: Howevewif.
there. is an excessive metabolism. offat; the-intermediate acetone bodies accumulate" inthe blood and are excretedrinthe urineinzvariable amounts. In diabetes mellitus; such an: excessive fat. metabolism occurs and.manyofsthe symp toms of the-disease can. be ascribed?tothe-toxic-.-
effects of the acetone bodies.- The medicalqpram titioner is well aware of the usef-ulnessiofxtests-r for acetonebodiesin theurinein diabetes-.; mel-.-.
litus.. Acetone bodies'also occurtin the urine in other Well. recognized disturbances of -metabolism, and in such cases also it is important to carry out tests for these substances.
In the past a varietyof reagents and techniques. have been used for the demonstration. of acetone'bodies in urine.
of asoluble .ni'troprussidev asa reactive ingredi ent or agent. Inonemodificati'o'n the nitr prusside reaction is carriedout'in the presence of ammonia in order to develop particular colorations. (See Patent'No. 2;186,902 to Fortune.) Careful study of availablereagents andctechniques for: detecting acetone indicates that theyaall are subject to onc or :more' of the :followin gr drawbacks or disadvantages:
1. A plurality of reagents arerequiredvfor most/rtests.
2. Most: testsrequire the skill of a: trained.-
technician.
3: Reagents :employed for most tests are unstable, poisonous and inconvenient to use;
A number of such" reagents. andv techniques have involved the use.
6 Claims: '(Cl. 252 -4403) 4. "Tests-accordingto" most techniques can only satisfactorily be performed in a laboratory.
5. Powder or dry reagent preparations-which contain an ammonia yielding ingredient are inherently unstable and-liberate ammonia and change" color .even when stored. in air-tight containe'rs.
6. Most dry. preparations evolvev noxious ammonia'duringthe performance of'the tests.
'7. Most 1 dry reagent preparations give a test with 'negativeurines which. maybe confusedwith a positive ,even' by a skilled operator."
The object of the present invention, generallystated; is theprovision. of diagnostic compositions: in stabledry 'io'rm; preferably tablets, which can be. used even-by an unskilled person conveniently and readily 'to give an accurate qualitative test for, anda quantitative estimation' of," the presence'of 'acetone bodies in .urine, which test clearly distinguishes between posi tive and negative specimens.
An. important object ofithe invention is the provision. of a "drwdiagnostic composition for the detection of acetonebodies "in urine-whichdoes not evolve ammonia either: in the dry state or "in-'the'wet'state; Certain otherobj'ects of the invention will, in-part,-be-obvious' and-wilt in part appear hereinafter;
Fora more complete understanding of thenature and scope of the invention. reference'may now be had to the followi'ngdetai leddescriptime thereofsettirig forth; by way of illustration; certain specific formulations which i constitute the presently' preferredembodiments of the in vention;
According to the present invention itnasbeen found that a solublenitro r-usside in. the ros ence of an aliphatic amino acid under alkaline: conditions; provides a diagnostid composition which is particularly adapted for the detection of acetone inurine: This diagnostic or zreagent composition: may beformulate'd in dry form; most suitabl lastablets.
Wneniac'etone positive specimens are tested" -withi this-diagnostic..compositiomia very markedi and definlte purple. reaction: or coloration-is prod'u-c'ed colorationqisxhighly characteristic and specific to acetone-positive specimens;
An important property-ref: the diagnostic compositions-isgthe-ifact that: acetone negative specie positions of the present invention should be made within one minute of the time that the powder or tablet is moistened with the specimen under examination.
The aliphatic amino acid of choice for the diagnostic compositions of the present invention is glycine (i. e. glycocol or amino acetic acid). This compound gives a. high quality coloration and has no blank color. Other aliphaticamino acids which give bright colors and which may be used in the diagnostic compositions are: alanine, glutamic acid, arginine, aspartic acid and lysine.
As mentioned above, alkaline conditions are required for testin specimens for acetone bodies in accordance with the invention. The a1- kaline material of choice for providing such alkalinity is disodium phosphate. With a dry powder or tablet preparation it is possible to use other alkaline solids such as carbonates, hy-
droxides, trisodium phosphate, dipotassium phos-. Instead of using a sepa-.
phate and the like. rate ingredient for imparting alkalinity, it is possible to use the alkali metal salts of the amino acids such as sodium or potassium glycinate. However, for practical purposes, disodium phosphate is the substance of choice since it is readily obtained as an anhydrous salt and its action in the test appears to be superior to that of a number of the other alkaline materials which can be used.
Any soluble nitroprusside appears to be useful in the reagent composition. Thus, any one of the alkali metal nitroprussides can be used. How-.
ever, sodium nitroprusside is the one of choice since it gives excellent results and is readily available on the chemical market.
It is possible, and often desirable, to use diluents along with the active constituents of the diagnostic compositions. Such diluents which have been satisfactorily used include starch, lactose, the borates, specifically sodium borate,
and a variety of other white or colorless com carry out a test since they are easily handled and may be conveniently disposed of.
However, the diagnostic compositions may, if desired, be used in powder or granular form and because of their stability in aqueous solution, the
compositions may even be used in liquid form.
The proportions of the amino acid, soluble nitroprusside and alkaline material, are not critical within broad limits, and these ingredients.
4 may be employed within a rather wide range of proportions. However, as the proportions of the ingredients in the diagnostic formulations are varied, it has been noted that the properties of the formulations are altered in the following respects:
1. The speed of reaction of the reagent with acetone positive urines.
2. The sensitivity of the reagent in detecting :small quantities of acetone bodies.
3. The presence or absence of a blank color with acetone negative urines. 4. The stability of the reagent when stored under adverse environmental conditions.
5. The quality and quantity of color obtained with urines having given concentrations of acei-tone bodies.
When sodium nitroprusside is used as the soluble nitroprusside in the diagnostic composition,
this ingredient should not comprise over approximately 5% by weight of the total bulk of the mixture and should represent at least 0.04% of the total bulk thereof. The preferred range of concentration is from 0.5% to 1%. Other soluble nitroprussides are preferably used in equivalent concentrations.
The relative proportions of disodium ph-os-' phate and glycine, or their equivalents, in the reagent compositions can vary quite widely and still the reagent will give satisfactory results with both acetone positive and acetone negative urines.
For instance, mixtures where the amount of' glycine was four times that of the sodium phos phate (i. e. circa glycine) gave clear cut tests. On the other hand, good tests were also obtained when the amount of glycine was only about 1% of that of the disodium phosphate. The optimum range of concentrations of the three preferred components is as follows in per cent by weight:
g Per cent Sodium nitroprusside 0.04- 5 Glycine 1.00-80 Disodium phosphate 20.00-99 When diluents are employed, they do not serve to'afiect the above preferred concentrations of the components except that the sodium nitroprusside or its equivalent should represent at least 0.04% of the total bulk. It will also be understood that equivalent components may be substituted in equivalent concentrations.
The two presently preferred embodiments of the invention are formulated as follows:
Example 1 50 grams of disodium phosphate, anhydrous,
50 grams of sodium borate, 2.5 gramsof glycine, and 0.8 gram of sodium nitroprusside are mixed together to give complete uniformity and this.-
mixture tabletted into tablets weighing approximately 0.3 gram each. Each of the tablets canbe used as a reagent in a test for acetone bodies in urine.
The preferred technique for testingspecimens for acetone using the diagnostic compositions of the present invention involves placing a tablet, or an equivalent pile of powder, on a flat surface and moistening the tablet or powder deposite with one drop of the urine specimen under examination. Within less than five seconds, acetone-containing urines cause the tablet or powder to acquire a characteristic type of purple or violet color, the intensity or shade of the color being proportional to the concentration of acetone bodies present. With negative urine samples the tablet or powder either does not undergo any color change or may assume a pale yellow or brown color which is easily diiferentiated from the characteristic purple color of the positive specimen.
Diagnostic compositions made in accordance with the present invention have been used clinically to study a series of acetone-containing urines from hospital patients as well as normal urines from healthy individuals. In all instances the urines were examined and checked by previously established methods as well as with the new reagent. The results of these clinical studies clearly showed that the diagnostic compositions of the present invention were particularly suited for use in detecting ketonuria and also for estimating the amount of ketone bodies in the urine. The clinical study emphasized the fact that the reagent formulations of the present invention were very reliable and that there was no difiiculty occasioned due to the confusion of the results of tests with negative urines and with positive urines.
The diagnostic compositions of the present invention have a number of advantages which increase their Value as reagents for testing specimens for the presence of acetone. Thus, the combination of disodium phosphate and glycine, or the equivalents thereof suggested above, comprises an extremely well buffered system that is unafiected by the reaction of the urine being tested. When the tests are made with the diagnostic compositions, n0 objectionable fumes of ammonia or other noxious gas are evolved as in the case with dry reagent compositions of the prior art. This is an important feature since in many instances, a technician will be testin a large number of specimens at the same time, often in a small room without adequate ventilation.
The combination of disodium phosphate and glycine, or their equivalents, is not reactive and is, accordingly, very stable. The anhydrous sodium phosphate is capable of taking up small amounts of water without deterioration so that the combination remains fully reactive even under adverse conditions of humidity,
Having thus fully described the invention and set forth formulations representing the preferred embodiments thereof, what is claimed as new is:
1. A diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia, consisting essentially of, from 0.04-5.0% of sodium nitroprusside, from 1-80% of glycine, and from 20-99% of disodium phosphate.
2. A diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia, consisting essentially of, approximately 5 parts of glycine, approximately 20 parts of disodium phosphate, and approximately 0.2 of a part of sodium nitroprusside.
3. A diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia, consisting essentially of, approximately 2.5 parts of glycine, approximately parts of disodium phosphate, approximately 0.8 part of sodium nitroprusside, and approximately 50 parts of sodium borate.
4. Diagnostic tables tabletted from the composition called for in claim 3.
5. A diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia, consisting essentially of from 0.04-5.0% of water soluble nitroprusside, from 1-80% of an aliphatic amino acid, and from 20-99% of an alkaline material.
6. A diagnostic composition in solid dry form for detecting acetone bodies in urine without evolving ammonia, consisting essentially of from 0.045.0% of an alkali metal nitroprusside, from 1-80% of an aliphatic amino acid, and from 20-99% of an alkaline alkali metal phosphate.
ALFRED H. FREE.
REFERENCES CITED The following references are of record in the file of this patent:
UNITED STATES PATENTS Number Name Date 2,171,962 Fortune Sept. 5, 1939 2,186,902 Fortune Jan. 9, 1940 2,283,262 Kamlet May 19, 1942 2,362,478 Galat Nov. 14, 1944 OTHER REFERENCES Gregory: Uses and Applications of Chemicals and Related Materials, vol. I, Reinhold Pub. 00., N. Y. C. (1939), page 112, column 2.
Kolthoff et al.: Acid-Base Indicators, The Mac- Millan 00., N. Y. (1937), pages 247-252 inclusive.
Claims (1)
1. A DIAGNOSTIC COMPOSITION IN SOLID DRY FORM FOR DETECTING ACETONE BODIES IN URINE WITHOUT EVOLVING AMMONIA, CONSISTING ESSENTIALLY OF, FROM 0.04-5.0% OF SODIUM NITROPRUSSIDE, FROM 1-80% OF GLYCINE, AND FROM 20-99% OF DISODIUM PHOSPHATE.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12699A US2509140A (en) | 1948-03-02 | 1948-03-02 | Test reagent composition |
GB28174/48A GB651448A (en) | 1948-03-02 | 1948-10-29 | Improvements in or relating to diagnostic compositions |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12699A US2509140A (en) | 1948-03-02 | 1948-03-02 | Test reagent composition |
Publications (1)
Publication Number | Publication Date |
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US2509140A true US2509140A (en) | 1950-05-23 |
Family
ID=21756271
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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US12699A Expired - Lifetime US2509140A (en) | 1948-03-02 | 1948-03-02 | Test reagent composition |
Country Status (2)
Country | Link |
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US (1) | US2509140A (en) |
GB (1) | GB651448A (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2577978A (en) * | 1949-02-04 | 1951-12-11 | Miles Lab | Diagnostic composition |
DE1153920B (en) * | 1957-10-21 | 1963-09-05 | Miles Lab | Diagnostic agent for the determination of ketone compounds in body fluids |
US3212855A (en) * | 1962-08-06 | 1965-10-19 | Miles Lab | Diagnostic device |
US3880590A (en) * | 1973-11-08 | 1975-04-29 | Shionogi & Co | Test strip for ketone bodies |
US4147514A (en) * | 1977-11-21 | 1979-04-03 | Miles Laboratories, Inc. | Test means and method for detecting ketone bodies |
US4172049A (en) * | 1977-05-13 | 1979-10-23 | Behringwerke Aktiengesellschaft | Control-solution for diagnostic detection methods for substances contained in the urine |
US4193766A (en) * | 1978-11-13 | 1980-03-18 | Miles Laboratories, Inc. | Device and method for preparation of a control solution for ketone determination |
US4405721A (en) * | 1980-03-22 | 1983-09-20 | Behringwerke Aktiengesellschaft | Diagnostic agent for the detection of ketone bodies |
US4517301A (en) * | 1982-12-06 | 1985-05-14 | Miles Laboratories, Inc. | Ketone control test composition, method and test device |
US4970172A (en) * | 1986-12-22 | 1990-11-13 | Abbott Laboratories | Method and device for ketone measurements |
US5071769A (en) * | 1986-12-22 | 1991-12-10 | Abbott Laboratories | Method and device for ketone measurement |
WO2012122394A1 (en) * | 2011-03-08 | 2012-09-13 | Akers Biosciences, Inc. | Breath ketone detector |
US9518991B2 (en) | 2012-05-15 | 2016-12-13 | Invoy Technologies, Llc | Method and apparatus for analyzing acetone in breath |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2171962A (en) * | 1938-12-19 | 1939-09-05 | Lilly Co Eli | Urine albumin test |
US2186902A (en) * | 1939-04-07 | 1940-01-09 | Lilly Co Eli | Urine acetone test |
US2283262A (en) * | 1940-10-02 | 1942-05-19 | Miles Lab | Diagnostic composition and method |
US2362478A (en) * | 1941-12-24 | 1944-11-14 | Denver Chemical Mfg Company | Reagent for testing for acetone |
-
1948
- 1948-03-02 US US12699A patent/US2509140A/en not_active Expired - Lifetime
- 1948-10-29 GB GB28174/48A patent/GB651448A/en not_active Expired
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2171962A (en) * | 1938-12-19 | 1939-09-05 | Lilly Co Eli | Urine albumin test |
US2186902A (en) * | 1939-04-07 | 1940-01-09 | Lilly Co Eli | Urine acetone test |
US2283262A (en) * | 1940-10-02 | 1942-05-19 | Miles Lab | Diagnostic composition and method |
US2362478A (en) * | 1941-12-24 | 1944-11-14 | Denver Chemical Mfg Company | Reagent for testing for acetone |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2577978A (en) * | 1949-02-04 | 1951-12-11 | Miles Lab | Diagnostic composition |
DE1153920B (en) * | 1957-10-21 | 1963-09-05 | Miles Lab | Diagnostic agent for the determination of ketone compounds in body fluids |
US3212855A (en) * | 1962-08-06 | 1965-10-19 | Miles Lab | Diagnostic device |
US3880590A (en) * | 1973-11-08 | 1975-04-29 | Shionogi & Co | Test strip for ketone bodies |
US4172049A (en) * | 1977-05-13 | 1979-10-23 | Behringwerke Aktiengesellschaft | Control-solution for diagnostic detection methods for substances contained in the urine |
US4147514A (en) * | 1977-11-21 | 1979-04-03 | Miles Laboratories, Inc. | Test means and method for detecting ketone bodies |
US4193766A (en) * | 1978-11-13 | 1980-03-18 | Miles Laboratories, Inc. | Device and method for preparation of a control solution for ketone determination |
US4405721A (en) * | 1980-03-22 | 1983-09-20 | Behringwerke Aktiengesellschaft | Diagnostic agent for the detection of ketone bodies |
US4517301A (en) * | 1982-12-06 | 1985-05-14 | Miles Laboratories, Inc. | Ketone control test composition, method and test device |
US4970172A (en) * | 1986-12-22 | 1990-11-13 | Abbott Laboratories | Method and device for ketone measurements |
US5071769A (en) * | 1986-12-22 | 1991-12-10 | Abbott Laboratories | Method and device for ketone measurement |
WO2012122394A1 (en) * | 2011-03-08 | 2012-09-13 | Akers Biosciences, Inc. | Breath ketone detector |
JP2014509732A (en) * | 2011-03-08 | 2014-04-21 | エイカーズ バイオサイエンシス インコーポレイテッド | Breath ketone detector |
US8871521B2 (en) | 2011-03-08 | 2014-10-28 | Akers Biosciences, Inc. | Breath ketone detector |
US9518991B2 (en) | 2012-05-15 | 2016-12-13 | Invoy Technologies, Llc | Method and apparatus for analyzing acetone in breath |
US10352940B2 (en) | 2012-05-15 | 2019-07-16 | Invoy Holdings, Llc | Method and apparatus for analyzing acetone in breath |
US11353462B2 (en) | 2012-05-15 | 2022-06-07 | Invoy Holdings Inc. | Method and apparatus for analyzing acetone in breath |
Also Published As
Publication number | Publication date |
---|---|
GB651448A (en) | 1951-04-04 |
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