US2619448A - Containers for preservation of bacteria cultures - Google Patents
Containers for preservation of bacteria cultures Download PDFInfo
- Publication number
- US2619448A US2619448A US139760A US13976050A US2619448A US 2619448 A US2619448 A US 2619448A US 139760 A US139760 A US 139760A US 13976050 A US13976050 A US 13976050A US 2619448 A US2619448 A US 2619448A
- Authority
- US
- United States
- Prior art keywords
- ampule
- container
- preservation
- bottle
- thread
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D83/00—Containers or packages with special means for dispensing contents
- B65D83/0055—Containers or packages provided with a flexible bag or a deformable membrane or diaphragm for expelling the contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/32—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents for packaging two or more different materials which must be maintained separate prior to use in admixture
- B65D81/3216—Rigid containers disposed one within the other
- B65D81/3222—Rigid containers disposed one within the other with additional means facilitating admixture
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/879—Salmonella
Definitions
- the invention relates to improvements in containers for preservation of bacteria cultures, especially for the destruction of vermin such as rats and mice.
- the object of the invention is to provide a container of the kind mentioned in which the bacteria culture may be preserved for a long time and easily be transplanted to a substratum for further development in view of subsequent use.
- Another object of the invention is to provide a container which ensures an absolutely sterilized handling of the bacteria culture until it is taken out of the container for use.
- a further object of the invention is to provide a container consisting of simple means to be produced at low costs, and which container does not necessitate intricate operations to be done when the bacteria are to be transplanted to the substratum.
- the container is a bottle I filled with a substratum 2, partly shown in dotted lines in the figure, e. g. a peptone-bouillon solution. Furthermore the bottle contains ampule 3 of a transparent glass having such internal tensions that it will crush to dust on being broken and having an external narrow pass 4.
- the ampule contains a bacteria culture, e. g. of the Salmonella typhi-murium well known for destruction of rats and mice.
- the contents of its two halves When breaking the ampule the contents of its two halves will quickly disperse in the substratum 2. While ordinary glass may be used, this dispersion will be accelerated if the ampule consists of glass having such internal tensions that it is crushed to dust when broken. This property may be imparted to the glass by quickly cooling the molten glass article as by dipping it in water.
- a container for the preservation of bacteria culture comprising a bottle having a narrow neck portion and a sterile closure, an ampule within said bottle enclosing a bacteria culture, said ampule having an external narrow pass in the middle thereof, a thread or wire extending through said closure and around said narrow pass in such manner as to hold said ampule in a transverse position near the narrow neck of the bottle so that when said thread is pulled said ampule will break against the neck of said bottle.
- Container for the preservation of bacteria cultures said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken.
- Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken whereby the contents of said ampule will be discharged into a substratum within said container, said ampule being composed of glass having such internal tensions that it will crush to dust when broken.
- Container for the preservation of bacteria cultures, said container having a narrow mouth forming a shoulder and a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule .and through said sterile closure, the length of said ampule exceeding the internal width of the mouth of said container, the thread being of such length as to suspend the ampule in contact with the shoulders of said container so that when said thread is pulled said ampule will break against the shoulders of said container.
Description
NOV. 25, 1952 S N 26l9448 CONTAINERS FOR PRESERVATION OF BACTERIA CULTURES Filed Jan. 20, 1950 v INVENTOR. Gasiay [arsen Patented Nov. 25, 1952 CONTAINERS FOR PRESERVATION F BACTERIA CULTURES Gustav Larsen, Haslev, Denmark Application January 20, 1950, Serial No. 139,760 In Denmark January 21, 1949 4.- Claims.
The invention relates to improvements in containers for preservation of bacteria cultures, especially for the destruction of vermin such as rats and mice.
The object of the invention is to provide a container of the kind mentioned in which the bacteria culture may be preserved for a long time and easily be transplanted to a substratum for further development in view of subsequent use.
Another object of the invention is to provide a container which ensures an absolutely sterilized handling of the bacteria culture until it is taken out of the container for use.
A further object of the invention is to provide a container consisting of simple means to be produced at low costs, and which container does not necessitate intricate operations to be done when the bacteria are to be transplanted to the substratum.
These and further objects and advantages of the invention will be apparent from the following detailed specification.
The accompanying drawing illustrates a preferred embodiment of the invention.
In the drawing the container is a bottle I filled with a substratum 2, partly shown in dotted lines in the figure, e. g. a peptone-bouillon solution. Furthermore the bottle contains ampule 3 of a transparent glass having such internal tensions that it will crush to dust on being broken and having an external narrow pass 4. The ampule contains a bacteria culture, e. g. of the Salmonella typhi-murium well known for destruction of rats and mice.
Around the narrow pass 4 there is a thread or wire 5 which while keeping the container sterilized is taken out through the sealing of the container, which consists of a sterilized closing plug 6. In this way the bacteria culture can be preserved for several years, and shortly before its use the ampule 3 is broken by pulling the thread or wire 5 whereby the ends of the ampule first contact the shoulders I of the bottle and then the ampule is broken so that its content is poured out into the substratum 2 where it disperses and starts development without the container having been opened.
One day or two later the whole contents of the bottle are useful for destruction of rats and mice during the following month.
When breaking the ampule the contents of its two halves will quickly disperse in the substratum 2. While ordinary glass may be used, this dispersion will be accelerated if the ampule consists of glass having such internal tensions that it is crushed to dust when broken. This property may be imparted to the glass by quickly cooling the molten glass article as by dipping it in water.
It is to be understood that the form of my invention, herewith shown and described, is to be taken as a preferred example of the same, and
2 that various changes are possible within the scope of the claims, without departing from the spirit Of my invention.
What I claim and desire .to secure by Letters Patent is:
1. A container for the preservation of bacteria culture comprising a bottle having a narrow neck portion and a sterile closure, an ampule within said bottle enclosing a bacteria culture, said ampule having an external narrow pass in the middle thereof, a thread or wire extending through said closure and around said narrow pass in such manner as to hold said ampule in a transverse position near the narrow neck of the bottle so that when said thread is pulled said ampule will break against the neck of said bottle.
2. Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken.
3. Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken whereby the contents of said ampule will be discharged into a substratum within said container, said ampule being composed of glass having such internal tensions that it will crush to dust when broken.
4. Container for the preservation of bacteria cultures, said container having a narrow mouth forming a shoulder and a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule .and through said sterile closure, the length of said ampule exceeding the internal width of the mouth of said container, the thread being of such length as to suspend the ampule in contact with the shoulders of said container so that when said thread is pulled said ampule will break against the shoulders of said container.
GUSTAV LARSEN.
REFERENCES CITED The following references are of record in the file of this patent:
UNITED STATES PATENTS Number Name Date 418,271 Buckmaster Dec. 31, 1889 1,332,985 Jarrett Mar. 9, 1920 1,991,938 Houghton Feb. 19, 1935 2,034,986 Mislowitzer Mar. 24, 1936 2,275,567 Smith Mar. 10, 1942
Claims (1)
1. A CONTAINER FOR THE PRESERVATION OF BACTERIA CULTURE COMPRISING A BOTTLE HAVING A NARROW NECK PORTION AND A STERILE CLOSURE, AN AMPULE WITHIN SAID BOTTLE ENCLOSING A BACTERIA CULTURE, SAID AMPULE HAVING AN EXTERNAL NARROW PASS IN THE MIDDLE THEREOF, A THREAD OR WIRE EXTENDING THROUGH SAID CLOSURE AND AROUND SAID NARROW PASS IN SUCH MANNER AS TO HOLD SAID AMPULE IN A TRANSVERSE POSITION NEAR THE NARROW NECK OF THE BOTTLE SO THAT WHEN SAID THREAD IS PULLED SAID AMPULE WILL BREAK AGAINST THE NECK OF SAID BOTTLE.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK2619448X | 1949-01-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
US2619448A true US2619448A (en) | 1952-11-25 |
Family
ID=8158582
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US139760A Expired - Lifetime US2619448A (en) | 1949-01-21 | 1950-01-20 | Containers for preservation of bacteria cultures |
Country Status (1)
Country | Link |
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US (1) | US2619448A (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2717226A (en) * | 1950-04-11 | 1955-09-06 | Skovsted Aage Thorsen | Process for the manufacture of a rat exterminating compound containing desiccated, storable salmonella bacteria in their virulent condition |
US2731965A (en) * | 1956-01-24 | haralson | ||
US2752036A (en) * | 1954-03-29 | 1956-06-26 | Jr John C Parkhurst | Container for chemicals |
US2776242A (en) * | 1952-05-23 | 1957-01-01 | Asta Werke Ag Chem Fab | Apparatus for providing fresh cultures of living micro-organisms |
US2971576A (en) * | 1957-09-30 | 1961-02-14 | Willard B Anker | Multiple tank assembly |
US3068154A (en) * | 1959-11-04 | 1962-12-11 | Hill Top Res Inst Inc | Apparatus for preparing a fresh culture of microorganisms |
US3616896A (en) * | 1969-07-15 | 1971-11-02 | Frederic D Barton | Cooling system package |
DE2461617A1 (en) * | 1973-12-27 | 1975-07-10 | Oreal | PRESSURELESS CONTAINER TO STORE SEPARATELY AND DISPOSE OF AT LEAST TWO MEDIA AT THE SAME TIME |
US4304869A (en) * | 1980-05-27 | 1981-12-08 | American Sterilizer Company | Apparatus for rupturing a sealed, frangible container |
US4387803A (en) * | 1980-11-21 | 1983-06-14 | Mercil Robert A | Absorbent device for containers for sugar, salt, etc. |
US4563186A (en) * | 1984-04-05 | 1986-01-07 | Purex Corporation | Multi-functional laundry product and employment of same during fabric laundering |
US4618444A (en) * | 1984-09-17 | 1986-10-21 | Purex Corporation | Household laundry detergent with dual strength bleach |
US5710041A (en) * | 1996-06-03 | 1998-01-20 | Remel L.P. | In situ reconstitutable lyophylized bacteria ampoule package |
US5733777A (en) * | 1996-06-06 | 1998-03-31 | Dudney; Ralph A. | Packaging system for extended shelf life of microbial system |
US6818438B2 (en) | 2002-10-04 | 2004-11-16 | Becton, Dickinson And Company | Culture flask |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US418271A (en) * | 1889-12-31 | Ligature-holder | ||
US1332985A (en) * | 1919-09-12 | 1920-03-09 | Edwin S Jarrett | Mixing-container |
US1991938A (en) * | 1926-04-05 | 1935-02-19 | Safety Fumigant Company | Volatile fumigant package |
US2034986A (en) * | 1932-01-02 | 1936-03-24 | Mislowitzer Ernst | Device for dispatching swabs, blood samples, and the like for bacteriological and diagnostic purposes |
US2275567A (en) * | 1933-11-03 | 1942-03-10 | Arthur E Smith | Container closure |
-
1950
- 1950-01-20 US US139760A patent/US2619448A/en not_active Expired - Lifetime
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US418271A (en) * | 1889-12-31 | Ligature-holder | ||
US1332985A (en) * | 1919-09-12 | 1920-03-09 | Edwin S Jarrett | Mixing-container |
US1991938A (en) * | 1926-04-05 | 1935-02-19 | Safety Fumigant Company | Volatile fumigant package |
US2034986A (en) * | 1932-01-02 | 1936-03-24 | Mislowitzer Ernst | Device for dispatching swabs, blood samples, and the like for bacteriological and diagnostic purposes |
US2275567A (en) * | 1933-11-03 | 1942-03-10 | Arthur E Smith | Container closure |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2731965A (en) * | 1956-01-24 | haralson | ||
US2717226A (en) * | 1950-04-11 | 1955-09-06 | Skovsted Aage Thorsen | Process for the manufacture of a rat exterminating compound containing desiccated, storable salmonella bacteria in their virulent condition |
US2776242A (en) * | 1952-05-23 | 1957-01-01 | Asta Werke Ag Chem Fab | Apparatus for providing fresh cultures of living micro-organisms |
US2752036A (en) * | 1954-03-29 | 1956-06-26 | Jr John C Parkhurst | Container for chemicals |
US2971576A (en) * | 1957-09-30 | 1961-02-14 | Willard B Anker | Multiple tank assembly |
US3068154A (en) * | 1959-11-04 | 1962-12-11 | Hill Top Res Inst Inc | Apparatus for preparing a fresh culture of microorganisms |
US3616896A (en) * | 1969-07-15 | 1971-11-02 | Frederic D Barton | Cooling system package |
US3964643A (en) * | 1973-12-27 | 1976-06-22 | L'oreal | Unpressurized container for holding a plurality of products separately and dispensing them simultaneously |
DE2461617A1 (en) * | 1973-12-27 | 1975-07-10 | Oreal | PRESSURELESS CONTAINER TO STORE SEPARATELY AND DISPOSE OF AT LEAST TWO MEDIA AT THE SAME TIME |
US4304869A (en) * | 1980-05-27 | 1981-12-08 | American Sterilizer Company | Apparatus for rupturing a sealed, frangible container |
US4387803A (en) * | 1980-11-21 | 1983-06-14 | Mercil Robert A | Absorbent device for containers for sugar, salt, etc. |
US4563186A (en) * | 1984-04-05 | 1986-01-07 | Purex Corporation | Multi-functional laundry product and employment of same during fabric laundering |
US4618444A (en) * | 1984-09-17 | 1986-10-21 | Purex Corporation | Household laundry detergent with dual strength bleach |
US5710041A (en) * | 1996-06-03 | 1998-01-20 | Remel L.P. | In situ reconstitutable lyophylized bacteria ampoule package |
US5733777A (en) * | 1996-06-06 | 1998-03-31 | Dudney; Ralph A. | Packaging system for extended shelf life of microbial system |
US6818438B2 (en) | 2002-10-04 | 2004-11-16 | Becton, Dickinson And Company | Culture flask |
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