US3649463A - Petri dish - Google Patents

Petri dish Download PDF

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Publication number
US3649463A
US3649463A US865292A US3649463DA US3649463A US 3649463 A US3649463 A US 3649463A US 865292 A US865292 A US 865292A US 3649463D A US3649463D A US 3649463DA US 3649463 A US3649463 A US 3649463A
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Prior art keywords
flange
cover
dish
wall
side wall
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Expired - Lifetime
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US865292A
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Noel L Buterbaugh
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WHITTAKER MA BIOPRODUCTS Inc A MD CORP
Microbiological Associates Inc
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Microbiological Associates Inc
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Assigned to WHITTAKER M.A. BIOPRODUCTS, INC., A MD CORP. reassignment WHITTAKER M.A. BIOPRODUCTS, INC., A MD CORP. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: DYNASCIENCES CORPORATION, A PA CORP.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means

Definitions

  • a container for the culturing of micro-organisms comprises a transparent cover member which includes a substantially flat top portion which merges into a continuous depending side wall, and a transparent body member having a substantially flat bottom portion merging into BACKGROUND OF THE INVENTION Containers of the type commonly known as Petri dishes, for the cluture of micro-organisms, have long been employed for the growth of bacteria, molds, and the like on sterile solid culture media.
  • Petri dishes usually comprise a dish or body member having a flat bottom and a cylindrical upstanding wall, and a cover member having a fiat top with a downwardly depending side wall which fits over and surrounds the outside of the wall of the dish portion.
  • Petri dishes are usually transparent, and may be made of glass or of molded plastic material.
  • the cover wall usually extends downward close to the bottom of the dish.
  • a principal object of the invention is to provide a Petri dish which includes means for lifting the dish and cover without disturbing the cover. Still another object is to provide a transparent Petri dish having an integrally molded plastic cover member and culture dish member whereby contamination of the respective members by each other is minimized.
  • FIG. 1 is a view in perspective of the container cover and body member separated from each other;
  • FIG. 2 is a plan view of the body or dish member showing the peripheral flange and slots therein;
  • FIG. 3 is a cross-section of the dish member in association with the cover member.
  • FIG. 4 is a plan view of the body or dish member showing the peripheral flange without the slots.
  • FIG. 1 shows the Petri dish type container of the invention, which may be of any desired cross-sectional form, but in the embodiment shown is cylindrical in cross-section.
  • the container comprises a body or dish member 10, and a cover member 20 adapted to fit over and surround the outside of the wall of the body member.
  • the cover member 20 includes a substantially flat portion 21 which merges into a continuous depending side wall 22 having a lower edge 23.
  • the body member 10 has a bottom portion 11 which is substantially fiat, and which merges into an upstanding wall portion '12 which is substantially cylindrical and which is advantageously integral with said bottom portion.
  • the body member 10 is provided with a peripheral flange 13 attached thereto, which extends outwardly from the exterior of the wall 12 and which serves as a means of gripping the body member 10 in order to lift it and its contents, whether or not the cover member 20 is in place.
  • the flange is integral with the wall portion.
  • slots 14 there extends inwardly from the outer edge of the peripheral flange 13 a plurality of slots 14, which may be of any desired configuration, but are rectangular in the embodiment shown in FIGS. 1 and 2, and which extend inwardly from, and are spaced equidistantly around, the flange edge.
  • These slots 14 serve to permit drainage from the flange of any liquid which may accumulate thereon in case of spillage, thus minimizing contamination.
  • the peripheral flange 13 is positioned so as to be spaced away from the bottom of the dish a suitable distance to permit ready grasping of the flange edge by the fingers, but at the same time it is located at a distance from the top edge 15 of the wall 12 sufficient to prevent contact between the upper surface of the flange and the lower edge 23 of the cover side wall when the cover is in place. It is a special feature of the invention that the lower edge 23 of the cover wall does not come into contact with nor rest upon the flange surface. This serves to prevent the cover edge from being contaminated by the flange. In the embodiment shown in the drawings, the flange is positioned about two-thirds of the distance from the upper edge 15 of the dish wall, and about one-third of the distance from the bottom edge.
  • Both the cover and the container body may be made of a transparent plastic material, such as, for example, polystyrene, polymerized methyl styrene, methyl methacrylate, polypropylene and the like, for light weight and resistance to breakage, and to sterilizing temperatures.
  • a transparent plastic material such as, for example, polystyrene, polymerized methyl styrene, methyl methacrylate, polypropylene and the like, for light weight and resistance to breakage, and to sterilizing temperatures.
  • a culture medium which may either be of a semi-rigid consistency, such as gelatin or agar, and which is inoculated with the organism to be grown, or which may be a liquid medium, such as is used for the growth of cell cultures and tissue explants, and in which the risk of spillage is present; the cover is then applied to the dish.
  • a culture medium which may either be of a semi-rigid consistency, such as gelatin or agar, and which is inoculated with the organism to be grown, or which may be a liquid medium, such as is used for the growth of cell cultures and tissue explants, and in which the risk of spillage is present.
  • a transparent container for the culturing of micro organisms comprising:
  • a peripheral flange attached to and extending outwardly around the exterior of said body member wall, the outer edge of said flange extending beyond the exterior of wall of said cover member when the cover is applied to the body member, said flange being positioned at a distance above the bottom edge of the body member sufficient to permit grasping thereof and at a distance below the top edge of the body wall sufiicient to prevent contact between the upper surface of the flange and the lower edge of the cover wall.
  • a transparent container for the culturing of microorganisms comprising:
  • a body or dish member having a substantially flat bottom portion merging into an upstanding wall por- 4 tion adapted to receive and support said cover member; and (c) a peripheral flange attached to and extending outwardly around the exterior of said body member wall, the outer edge of said flange extending beyond the exterior of wall of said cover member when the cover is applied to the body member, said flange being positioned at a distance above the bottom edge of the body member sufficient to permit grasping thereof and at a distance below the top edge of the body wall suflicient to prevent contact between the upper surface of the flange and the lower edge of the cover wall, said flange including a plurality of slots extending inwardly from, and spaced equidistantly around the peripheral edge thereof.
  • the container of claim 1 which is a Petri dish. 4. The container of claim 1 in which the flange is integral with the body member wall.
  • the container of claim 1 which is made of transparent plastic.
  • the container of claim 2 which is a Petri dish.
  • the flange is integral with the body member wall.

Abstract

A CONTAINER FOR THE CULTURING OF MICRO-ORGANISMS COMPRISES A TRANSPARENT COVER MEMBER WHICH INCLUDES A SUB STANTIALLY FLAT TOP PORTION WHICH MERGES INTO A CONTINUOUS DEPENDING SIDE WALL, AND A TRANSPARENT BODY MEMBER HAVING A SUBSTANTIALLY FLAT BOTTOM PORTION MERGING INTO AN UPSTANDING CYLINDRICAL WALL PORTION WHICH CARRIES ON ITS OUTER SURFACE AN OUTWARDLY EXTENDING PERIPHERAL FLANGE PROVIDED WITH A PLURALITY OF INWARDLY EXTENDING SLOTS, SAID FLANGE BEING SPACED UPWARD FROM THE BOTTOM PORTION AND AWAY FROM THE LOWER EDGE OF SAID COVER SIDE WALL, THE OUTER EDGE OF SAID FLANGE EXTENDING BEYOND THE EXTERIOR OF SAID COVER SIDE WALL TO PERMIT GRASPING OF THE FLANGE BY THE FINGERS.

Description

March 14, 1972 N. L. BUTERBAUGH 3,649,463
PETRI DISH Filed Oct. 10,1969
FIG. 4
INVENTOR 3 NOEL L. BUTERBAUGH ATTORNEY United States Patent Olfice 3,649,463 PETRI DISH Noel L. Buterbaugh, Bethesda, Md., assignor to Microbiological Associates, Inc., Bethesda, Md.
Filed Oct. 10, 1969, Ser. No. 865,292
Int. Cl. C12k N US. Cl. 195139 9 Claims ABSTRACT OF THE DISCLOSURE A container for the culturing of micro-organisms comprises a transparent cover member which includes a substantially flat top portion which merges into a continuous depending side wall, and a transparent body member having a substantially flat bottom portion merging into BACKGROUND OF THE INVENTION Containers of the type commonly known as Petri dishes, for the cluture of micro-organisms, have long been employed for the growth of bacteria, molds, and the like on sterile solid culture media. Petri dishes usually comprise a dish or body member having a flat bottom and a cylindrical upstanding wall, and a cover member having a fiat top with a downwardly depending side wall which fits over and surrounds the outside of the wall of the dish portion. Petri dishes are usually transparent, and may be made of glass or of molded plastic material. The cover wall usually extends downward close to the bottom of the dish.
One of the major drawbacks of this conventional construction has been that, in order to lift the Petri dish, the user will customarily extend his fingers under the edge of the cover member and grasp the bottom edge of the body member. Since the fit is loose, the dish portion containing the culture may slip from the fingers, leaving the user holding only the cover, thus exposing the contents of the dish to contamination and loss. Another alternative for picking up the conventional Petri dish is to slide the entire dish off the edge of the surface on which it rests, but this is not feasible where the dish rests in a recess in a supporting tray.
GENERAL DESCRIPTION OF THE INVENTION In accordance with the present invention there is provided a novel contained for microorganism culture, and which is an improved Petri dish.
A principal object of the invention is to provide a Petri dish which includes means for lifting the dish and cover without disturbing the cover. Still another object is to provide a transparent Petri dish having an integrally molded plastic cover member and culture dish member whereby contamination of the respective members by each other is minimized.
These and other objects will become apparent as the description proceeds in the following part of the specification wherein the details of construction of a presently preferred embodiment are set forth, together with the accompanying drawings.
While the principles of the invention will be described with particular reference to their embodiment in a Petri 3,649,463 Patented Mar. 14, 1972 dish, it will be understood that such description is for purposes of illustration only, and that these principles are capable of adaptation to a variety of containers having the same general configuration and structure.
DESCRIPTION OF THE PREFERRED EMBODIMENT The novel features and operation of the Petri dish of the present invention will be better understood by reference to the accompanying drawings, in which:
FIG. 1 is a view in perspective of the container cover and body member separated from each other;
FIG. 2 is a plan view of the body or dish member showing the peripheral flange and slots therein;
FIG. 3 is a cross-section of the dish member in association with the cover member; and
FIG. 4 is a plan view of the body or dish member showing the peripheral flange without the slots.
Referring now to the drawings, FIG. 1 shows the Petri dish type container of the invention, which may be of any desired cross-sectional form, but in the embodiment shown is cylindrical in cross-section. The container comprises a body or dish member 10, and a cover member 20 adapted to fit over and surround the outside of the wall of the body member. The cover member 20 includes a substantially flat portion 21 which merges into a continuous depending side wall 22 having a lower edge 23.
The body member 10 has a bottom portion 11 which is substantially fiat, and which merges into an upstanding wall portion '12 which is substantially cylindrical and which is advantageously integral with said bottom portion. The body member 10 is provided with a peripheral flange 13 attached thereto, which extends outwardly from the exterior of the wall 12 and which serves as a means of gripping the body member 10 in order to lift it and its contents, whether or not the cover member 20 is in place. Preferably the flange is integral with the wall portion.
In a preferred embodiment of the invention, there extends inwardly from the outer edge of the peripheral flange 13 a plurality of slots 14, which may be of any desired configuration, but are rectangular in the embodiment shown in FIGS. 1 and 2, and which extend inwardly from, and are spaced equidistantly around, the flange edge. These slots 14 serve to permit drainage from the flange of any liquid which may accumulate thereon in case of spillage, thus minimizing contamination. Preferably there are 4 slots equally spaced around the flange.
The peripheral flange 13 is positioned so as to be spaced away from the bottom of the dish a suitable distance to permit ready grasping of the flange edge by the fingers, but at the same time it is located at a distance from the top edge 15 of the wall 12 sufficient to prevent contact between the upper surface of the flange and the lower edge 23 of the cover side wall when the cover is in place. It is a special feature of the invention that the lower edge 23 of the cover wall does not come into contact with nor rest upon the flange surface. This serves to prevent the cover edge from being contaminated by the flange. In the embodiment shown in the drawings, the flange is positioned about two-thirds of the distance from the upper edge 15 of the dish wall, and about one-third of the distance from the bottom edge.
Both the cover and the container body may be made of a transparent plastic material, such as, for example, polystyrene, polymerized methyl styrene, methyl methacrylate, polypropylene and the like, for light weight and resistance to breakage, and to sterilizing temperatures.
In use, there is introduced into the dish member a culture medium which may either be of a semi-rigid consistency, such as gelatin or agar, and which is inoculated with the organism to be grown, or which may be a liquid medium, such as is used for the growth of cell cultures and tissue explants, and in which the risk of spillage is present; the cover is then applied to the dish. When it is desired to transfer the Petri dish or to lift it for inspection, the edge of the flange is grasped with the fingers, the cover remaining in place undisturbed.
What is claimed is:
1. A transparent container for the culturing of micro organisms comprising:
(a) a cover member including a substantially flat top portion which merges into a continuous depending side wall;
(b) a body or dish member having a substantially flat bottom portion merging into an upstanding Wall portion adapted to receive and support said cover member; and
(c) a peripheral flange attached to and extending outwardly around the exterior of said body member wall, the outer edge of said flange extending beyond the exterior of wall of said cover member when the cover is applied to the body member, said flange being positioned at a distance above the bottom edge of the body member sufficient to permit grasping thereof and at a distance below the top edge of the body wall sufiicient to prevent contact between the upper surface of the flange and the lower edge of the cover wall.
2. A transparent container for the culturing of microorganisms comprising:
(a) a cover member including a substantially flat top portion which merges into a continuous depending side wall;
(b) a body or dish member having a substantially flat bottom portion merging into an upstanding wall por- 4 tion adapted to receive and support said cover member; and (c) a peripheral flange attached to and extending outwardly around the exterior of said body member wall, the outer edge of said flange extending beyond the exterior of wall of said cover member when the cover is applied to the body member, said flange being positioned at a distance above the bottom edge of the body member sufficient to permit grasping thereof and at a distance below the top edge of the body wall suflicient to prevent contact between the upper surface of the flange and the lower edge of the cover wall, said flange including a plurality of slots extending inwardly from, and spaced equidistantly around the peripheral edge thereof. 3. The container of claim 1 which is a Petri dish. 4. The container of claim 1 in which the flange is integral with the body member wall.
5. The container of claim 1 which is made of transparent plastic.
6. The container of claim 2 in which said slots are substantially rectangular in shape.
7. The container of claim 2 which is a Petri dish. 8. The container of claim 2 in which the flange is integral with the body member wall.
9. The container of claim 2 which is made of transparent plastic.
References Cited UNITED STATES PATENTS ALVIN E. TANENHOLTZ, Primary Examiner
US865292A 1969-10-10 1969-10-10 Petri dish Expired - Lifetime US3649463A (en)

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Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4160700A (en) * 1977-02-14 1979-07-10 Gelman Instrument Company Petri dish
FR2465978A1 (en) * 1979-09-25 1981-03-27 Pasteur Institut Melting heat-sensitive products in sterile conditions - using electromagnetic radiation in appts. partic. useful in preparing media for Petri dishes in culture of microorganisms
EP0160743A2 (en) * 1984-03-22 1985-11-13 Wadley Technologies, Inc. Miniaturized yeast identification system
US4743556A (en) * 1987-05-15 1988-05-10 Curtin Matheson Scientific, Inc. Petri dish
US4847128A (en) * 1984-03-22 1989-07-11 Wadley Technologies, Inc. Miniaturized yeast identification system
US5021351A (en) * 1983-05-02 1991-06-04 Becton, Dickinson And Company Petri dish
US5081033A (en) * 1984-03-22 1992-01-14 Wadley Technologies, Inc. Miniaturized yeast identification system
US5520302A (en) * 1994-01-27 1996-05-28 Anderson; Roger Petri dish having two-position lid
WO1997036992A1 (en) * 1996-03-29 1997-10-09 Corning Costar Corporation Culture dish
US6602704B1 (en) 2002-06-24 2003-08-05 Biomerieux, Inc. Sample contact plate with latchable cover
US20060252299A1 (en) * 2005-05-09 2006-11-09 Victor Daykin Contact plate
US20070020753A1 (en) * 2005-07-19 2007-01-25 The Ohio State University Condensation reducing/eliminating container
US20070148764A1 (en) * 2003-12-18 2007-06-28 Tsutomu Suzuki Device for cell culture
DE102009013673A1 (en) * 2009-03-09 2010-09-16 Eppendorf Ag Cell culture dish
WO2014111518A1 (en) * 2013-01-17 2014-07-24 Medibiome Ab Tissue bioreactor
WO2016074614A1 (en) 2014-11-11 2016-05-19 Thermo Fisher Scientific (Shanghai) Instrument Co., Ltd. Petri dish
EP3534138A1 (en) * 2018-03-01 2019-09-04 CleanControlling GmbH Transportable particle collecting device
US11680236B2 (en) 2017-11-30 2023-06-20 Corning Incorporated Thin, uniform, stackable petri dish

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4160700A (en) * 1977-02-14 1979-07-10 Gelman Instrument Company Petri dish
FR2465978A1 (en) * 1979-09-25 1981-03-27 Pasteur Institut Melting heat-sensitive products in sterile conditions - using electromagnetic radiation in appts. partic. useful in preparing media for Petri dishes in culture of microorganisms
US5021351A (en) * 1983-05-02 1991-06-04 Becton, Dickinson And Company Petri dish
EP0160743A2 (en) * 1984-03-22 1985-11-13 Wadley Technologies, Inc. Miniaturized yeast identification system
EP0160743A3 (en) * 1984-03-22 1986-04-30 J.K. And Susie L. Wadley Research Institute And Blood Bank Miniaturized yeast identification system
US4847128A (en) * 1984-03-22 1989-07-11 Wadley Technologies, Inc. Miniaturized yeast identification system
US5081033A (en) * 1984-03-22 1992-01-14 Wadley Technologies, Inc. Miniaturized yeast identification system
US4743556A (en) * 1987-05-15 1988-05-10 Curtin Matheson Scientific, Inc. Petri dish
US5520302A (en) * 1994-01-27 1996-05-28 Anderson; Roger Petri dish having two-position lid
WO1997036992A1 (en) * 1996-03-29 1997-10-09 Corning Costar Corporation Culture dish
US5856176A (en) * 1996-03-29 1999-01-05 Corning Incorporated Culture dish
US6602704B1 (en) 2002-06-24 2003-08-05 Biomerieux, Inc. Sample contact plate with latchable cover
US20040018616A1 (en) * 2002-06-24 2004-01-29 Maxwell Douglas E. Sample contact plate with latchable cover
US6764850B2 (en) 2002-06-24 2004-07-20 Biomerieux, Inc. Sample contact plate with latchable cover
US20070148764A1 (en) * 2003-12-18 2007-06-28 Tsutomu Suzuki Device for cell culture
US7754478B2 (en) * 2003-12-18 2010-07-13 Kaneka Corporation Device for cell culture
US20060252299A1 (en) * 2005-05-09 2006-11-09 Victor Daykin Contact plate
US7452711B2 (en) 2005-05-09 2008-11-18 Victor Daykin Contact plate
US20070020753A1 (en) * 2005-07-19 2007-01-25 The Ohio State University Condensation reducing/eliminating container
DE102009013673A1 (en) * 2009-03-09 2010-09-16 Eppendorf Ag Cell culture dish
US20110003376A1 (en) * 2009-03-09 2011-01-06 Eppendorf Ag Cell Culture Dish
US9040293B2 (en) 2009-03-09 2015-05-26 Eppendorf Ag Cell culture dish
WO2014111518A1 (en) * 2013-01-17 2014-07-24 Medibiome Ab Tissue bioreactor
WO2016074614A1 (en) 2014-11-11 2016-05-19 Thermo Fisher Scientific (Shanghai) Instrument Co., Ltd. Petri dish
EP3218468A4 (en) * 2014-11-11 2018-08-15 Thermo Fisher Scientific (Shanghai) Instrument Co. Ltd. Petri dish
US10920183B2 (en) 2014-11-11 2021-02-16 Thermo Fisher Scientific (Shanghai) Instrument Co., Ltd. Petri dish
US11680236B2 (en) 2017-11-30 2023-06-20 Corning Incorporated Thin, uniform, stackable petri dish
EP3534138A1 (en) * 2018-03-01 2019-09-04 CleanControlling GmbH Transportable particle collecting device

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Owner name: WHITTAKER M.A. BIOPRODUCTS, INC., 10880 WILSHIRE B

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:DYNASCIENCES CORPORATION, A PA CORP.;REEL/FRAME:004293/0130

Effective date: 19840726