US3717550A - Liquid compositions of bacterial protease and/or amylase and preparation thereof - Google Patents

Liquid compositions of bacterial protease and/or amylase and preparation thereof Download PDF

Info

Publication number
US3717550A
US3717550A US00075764A US3717550DA US3717550A US 3717550 A US3717550 A US 3717550A US 00075764 A US00075764 A US 00075764A US 3717550D A US3717550D A US 3717550DA US 3717550 A US3717550 A US 3717550A
Authority
US
United States
Prior art keywords
composition
enzyme
bacterial
weight
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US00075764A
Inventor
J Ziffer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PMP ACQUISITION Corp A DE CORP
Original Assignee
Pabst Brewing Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pabst Brewing Co filed Critical Pabst Brewing Co
Application granted granted Critical
Publication of US3717550A publication Critical patent/US3717550A/en
Assigned to FIRST WISCONSIN NATIONAL BANK OF MILWAUKEE, A NATIONAL BANKING ASSOCIATION, AS AGENT reassignment FIRST WISCONSIN NATIONAL BANK OF MILWAUKEE, A NATIONAL BANKING ASSOCIATION, AS AGENT SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: PABST BREWING COMPANY A DE CORP.
Assigned to PMP ACQUISITION CORPORATION, A DE CORP. reassignment PMP ACQUISITION CORPORATION, A DE CORP. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: PAPST BREWING COMPANY
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
    • D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
    • D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
    • D06L1/14—De-sizing
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions

Definitions

  • the problem is particularly acute in the manufacturing and handling of the enzymes themselves and also in the handling of the enzymes in plants where they are formulated into various forms of detergent products.
  • it has heretofore been proposed to produce a dust-free powdered enzyme product.
  • Another possible solution of the problem would be the provision of the enzymes in the form of a liquid composition.
  • Another problem arises the instability of the enzymes which causes the enzyme activity of a liquid product to be reduced in storage.
  • Another problem arises due to the fact that the enzymes as normally produced form turbid solutions in water containing suspended particles. It would, therefore, be desirable to solve the dusting problem which is a source of irritation to individuals by providing a liquid composition containing enzymes which retain a relatively high activity when the composition is stored prior to use.
  • liquid concentrates of bacterial protease and/or amylase of enhanced stability would avoid the source of irritation caused by the dusting of powdered enzymes and they could be used in the manufacture of various detergent products.
  • liquid enzyme compositions tree from turbidity would be acceptable for use as such in household detergent formulations and also as enzyme compositions for the treatment of textiles, for example, in the de-sizing of textile fibers.
  • One of the ob pets of the present invention is to provide new and improved liquid concentrates of bacterial protease and/or bacterial amylase of enhanced stability.
  • Another object of the invention is to provide liquid compositions containing bacterial protease and/or bacterial amylase which are free of turbidity.
  • Still a further object of the invention is to provide a new and improved process for producing liquid compositions containing bacterial protease and/or bacterial amylase.
  • Another ob ject of the invention is to provide new and improved liquid compositions-containing bacterial protease and/or bacterial amylase in combination with surfactants. Other objects will appear hereinafter.
  • liquid compositions consisting essentially of: Component (A),
  • fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and Component (B), a liquid from the group consisting of water, a normally liquid water miscible polyhydric alcohol, and both water and a normally liquid water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32 percent by weight fermentation soluble solids at 4.4C.
  • said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50 percent when said composition is stored in a container under atmospheric pressure for 6 days at 42C.
  • compositions of the invention will normally have a protease activity of at least 5,000 PV units per gram at 44C., preferably within the range of 5,000 to 2,000,000 PV units per gram at 44C., and generally within the range of 30,000 to 600,000 PV units per gram at 4.4C.
  • the amylase activity can be up to 1,000,000 DV units per gram at 4.4C., is preferably at least 10,000 DV units per gram at 44C., and is generally within the range of 30,000 to 400,000 DV units per gram at 4.4C.
  • the enzymes in the liquid compositions can consist essentially of bacterial protease or bacterial amylase or both bacterial protease and bacterial amylase in the above described proportions.
  • the liquid compositions can be used as such for washing or they can be mixed with other ingredients for the preparation of detergent compositions.
  • they can be mixed with various types of compatible surfactants to form liquid concentrates which can be added to wash water in predetermined amounts.
  • Component (B) is water
  • the stability of the enzymes is enhanced by maintaining a relatively high enzyme concentration, namely, a concentration of at least 32 percent by weight at 4.4C. (40F).
  • a polyhydric alcohol such as glycerol, either alone or mixed with water, constitutes the liquid phase
  • the retained activity of the enzymes over a period of time is enhanced even at enzyme concentrations below 32 percent by weight.
  • polyhydric alcohols for example, glycerol, propylene glycol, ethylene glycol, diethylene glycol and higher liquid homologues, have the property of removing the turbidity which is normally present in concentrated bacterial protease and bacterial amylase solutions in water.
  • the use of polyhydric alcohols in the preparation of these liquid compositions makes it possible to produce clear liquid compositions which do not leave deposits when employed in washing procedures and in textile treatment and are therefore more acceptable for such uses.
  • compositions in accordance with the invention in accordance with the invention several different methods may be used.
  • the usual procedure involves first preparing an aqueous concentrate by adding bacterial protease and bacterial amylase to water and agitating the resultant composition at room temperature (2032C.) so as to dissolve the enzyme.
  • the composition is then filtered and the filter cake washed with water.
  • the combined filtrate and wash water after concentration is a turbid solution, probably containing colloidal protein solid in suspension.
  • the concentration of enzyme solids can be within the range of 2 to 60 percent by weight and is usually within the range of 32 to 43.5 percent by weight.
  • the composition may contain 40 to 98 percent by weight glycerol.
  • these liquid enzyme compositions can be employed as such in washing by adding them to the wash water in the proper proportions. In most cases the proportions should be sufficient to give an enzyme concentration of ZOO-2,000 PV units/liter and -500 DV units/liter in a water having a pH within the range of 8 to 12.
  • Compatible surfactants can also be added to the liquid enzyme concentrates. For most purposes the surfactant is either a nonionic or an anionic surfactant. It may also be an amphoteric surfactant. Any of the compatible surfactants described in Me- Cutcheons Detergents and Emulsifiers, 1969 Annual, can be employed.
  • surfactants examples include Alrosperse DC, Triton QS-l5, QS-30, 136-5, and X-102. It is usually preferable to carry out routine experiments in order to determine the compatibility of a particular surfactant. Some of these surfactants are compatible with some polyhydric alcohols but not with others.
  • EXAMPLE 1 A 20 percent (w/v) solution of Al (SO 'l8H- O was slowly added to an agitated bacterial protease fermentation whole culture (500 gal, 10,720 PV units per ml., 20.3 X PV units) while maintaining the whole culture pH at 6.4 by the simultaneous addition of Na SO (42.5 lb.). The amount of Al (SO.,) -l8l-I O added was 1 percent'(w/v) based on the whole culture. Coagulants Nalco 674 (120 grams, as a 0.25 percent (w/v) solution) and Purifloc C-31(5,025 grams, as a 27 percent (w/v) solution were then added, together with filteraid Chem-Flo 325 CK (290 lbs.).
  • EXAMPLE 2 The bacterial protease filtered cake obtained in Example 1 after precipitation with Na SO Na SO and Nal-ISO was used to prepare a series of aqueous concentrates. Distilled water (7,200 ml.) was added to a portion of the filtered cake (1,765 grams, 680 X 10 PV units) and the mixture agitated at room temperature for 1.5 hours. The mixture was then filtered on a Buchner funnel using a Filteraid FW-20 pre-coat and the filtered cake washed with water (3,000 ml.). The combined filtrate and washed totaled 11,840 ml. and contained 620 X 10 PV units, for a protease enzyme recovery of 91.2 percent.
  • an aqueous concentrate was prepared having a per cent dry solids of 32 percent and a protease enzyme activity of 360,000 PV units per gram.
  • the resultant concentrate was a tan turbid solution containing suspended solid.
  • aqueous concentrate 100 grams was added to propylene glycol (20 grams, reagent grade) resulting in an almost complete clearing of the turbidity of the aqueous concentrate. Aliquots of this solution (without any further evaporation) were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 100 grams was added to propylene glycol (50 grams, reagent grade) and the mixture evaporated in vacuo to 100 grams final weight.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 100 grams was added to propylene glycol (50 grams, reagent grade) and the mixture evaporated in vacuo to 100 grams. An additional 20 grams of propylene glycol was then added to the clear solution resulting in a final total weight of 120 grams, of which 70 grams constituted propylene glycol. Aliquots of this clear solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 200 grams was added to ethylene glycol (100 grams, reagent grade) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 200 grams was added to glycerol (100 grams, U.S.P. grade) and the mixture evaporated in vacuo to 200 grams final weight.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • glycerol 50 32 6 340 k 325 it 95.6 21 340 k 315 k 92.6 43 355 k 286.4 k 80.7
  • EXAMPLE 4 Using the procedures described in Examples 1 and 2, an aqueous protease concentrate was prepared having a per cent dry solids of 38.3 percent and a protease enzyme activity of 355,000 PV units per gram. The resultant concentrate was a tan turbid solution containing suspended solid. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 200 grams was added to glycerol grams, U.S.P. grade) and the mixture evaporated in vacuo to 200 grams final weight.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • an aqueous concentrate was prepared having a per cent dry solids of 32 percent and a protease enzyme activity of 350,000 PV units per gram.
  • the resultant concentrate was a tan turbid solution containing suspended solid.
  • aqueous concentrate 400 grams was added to glycerol (128 grams, U.S.P. grade) and the mixture evaporated in vacuo to 294 grams.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 100 grams was added to glycerol (55 grams, U.S.P. grade) and the mixture evaporated in vacuo to 100 grams.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 100 grams was added to glycerol (60 grams, U.S.P. grade) and the mixture evaporated in vacuo to 100 grams.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • aqueous concentrate 120 grams was added to glycerol (48 grams, U.S.P. grade) and the mixture evaporated in vacuo to 98 grams.
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • EXAMPLE 6 Using the procedures described in Examples 1 and 2, an aqueous concentrate was prepared having a percent dry solids of 38.9 percent and a protease enzyme activity of 381,000 PV units per gram. The resultant product was a tan turbid solution containing suspended solids. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • glycerol 500 grams, U.S.P. grade
  • the resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
  • Portions of the glycerol-aqueous concentrate were then further diluted with additional glycerol in the glycerol concentration range of 50-99 percent of the total solution.
  • the resultant products were clear solutions. Aliquots of these solutions were tested at 4.4C. and 42C. for storage stability.
  • Amylase Activity (DV/Gm.) After 36 Days at Indicated Temperature Dry Solids ln Concentrate 4.4C. 42C.
  • EXAMPLE 8 a Using the procedures described in Example 3, a portion of the aqueous concentrate (200 grams) from Example 7 was added to glycerol grams, U.S.P. grade) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C., 28C., 42C. and 55C. for storage stability. The results obtained are shown below:
  • Amylase Activity (DV/Gm.) After non-glycerol 30 Days at Indicated glycerol dry Temperature in concentrate solids 4.4C. 28C. 42C. 55C.
  • Example 3 Using the procedures described in Example 3, a portion of the aqueous concentrate (450 grams) from Example 7 was added to glycerol (225 grams, U.S.P. grade) and the mixture evaporated in vacuo to 450 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 55C. for storage stability. The results obtained are An enzyme concentrate was prepared by mixing equal parts by weight of a bacterial protease-glycerol concentrate containing 50 percent glycerol by weight and having an activity of 464,000 PV units per gram with a bacterial amylase concentrate containing 50 percent glycerol by weight and having an activity of 90,680 DV units per gram and 20,000 PV units per gram.
  • the final product contained a protease concentration of 242,000 PV units per gram and 45,340 DV units per gram.
  • EXAMPLE 10 The procedure was the same as in Example 9 except that additional glycerol was added and the relative proportions of bacterial protease-glycerol concentrate, bacterial amylase-glycerol concentrate and glycerol were, respectively, 12.42 percent, 65.70 percent and 21.88 percent by weight. The final product had a protease concentration of 70,760 PV units per gram and 59,5 80 DV units per gram.
  • EXAMPLE 1 l The procedure was the same as in Example 9 except that additional glycerol was added and the relative proportions of bacterial protease-glycerol concentrate, bacterial amylase-glycerol concentrate and glycerol were 46.25 percent, 37.05 percent and 16.70 percent by weight, respectively.
  • the final product contained a protease concentration of 222,200 PV units per gram and 33,600 DV units per gram.
  • EXAMPLE 12 The bacterial protease-glycerol concentrate of Example 9 containing 50 percent by weight glycerol and having a protease concentration of 464,000 PV units per gram was mixed in a weight ratio of 4:1 with Alrospere DC, to produce a composition having a protease concentration of 371,200 PV units per gram.
  • EXAMPLE 13 The procedure was the same as in Example 12 except that 80 parts of the protease-glycerol concentrate was mixed with 5 parts Alrosperse DC and parts of additional glycerol. The protease concentration was the same as in Example 12.
  • EXAMPLE 14 The procedure was the same as in Example 12 except that the surfactant Triton 08-1 5 was used.
  • EXAMPLE 15 The procedure was the same as in Example 13 except that the surfactant Triton 08-15 was used.
  • EXAMPLE 17 The procedure was the same as in Example 13 except that the surfactant Triton BG-S was used.
  • EXAMPLE 18 The procedure was the same as in Example 12 except that a bacterial amylase-glycerol concentrate containing 50 percent by weight glycerol was used instead of the bacterial protease-glycerol concentrate. Since the bacterial amylase contained some protease, the final product had an amylase concentration of 72,540 DV units per gram and a protease concentration of 16,000 PV units per gram.
  • EXAMPLE 19 The procedure was the same as in Example 13 except that a bacterial amylase-glycerol concentrate of the type employed in Example 18 was used. The final product had an amylase concentration of 72,540 DV units per gram and a protease concentration of 16,000 PV units per gram.
  • EXAMPLE 20 The procedure was the same as in Example 18 except that Triton QS-l 5 was used instead of Alrosperse DC.
  • EXAMPLE 21 The procedure was the same as in Example 19 except that Triton 08-15 was used instead of Alrosperse DC.
  • EXAMPLE 22 The procedure was the same as in Example 18 except that Triton BG-S was used instead of Alrosperse DC.
  • EXAMPLE 23 The procedure was the same as in Example 19 except that Triton BG-S was used instead of Alrosperse DC.
  • EXAMPLE 25 The procedure was the same as in Example 24 except that Triton BG-S was substituted for the Alrosperse DC.
  • EXAMPLE 26 The procedure was the same as in Example 24 except that Triton QS-15 was substituted for the Alrosperse DC.
  • the bacterial amylase enzyme normally contains some bacterial protease enzyme.
  • the protease enzyme activity is not given.
  • the Alrosperse DC is an oil soluble surfactant which is nonionic plus a cationic mixture of fatty alkoamides.
  • Triton BG-S is a non-ionic surfactant.
  • Triton QS-l 5 is a liquid anhydrous amphoteric surfactant in the form of an oxyethylated sodium salt containing both anionic and cationic centers. All of these surfactants are identified in McCutcheons 1969 Annual, supra.
  • the clarity of the enzyme solution depends upon the particular enzyme and the concentration thereof.
  • the addition of the polyhydric alcohol serves to reduce the turbidity and thereby increase the clarity of the solution. This does not necessarily mean, however, that one can see through a given sample of solution after it has been clarified.
  • the composition prior to treatment with the polyhydric alcohol will contain suspended particles which cause turbidity and will usually have a tan appearance whereas after the treatment with a polyhydric alcohol the turbidity will be reduced due to the dissolution of the particles and the resultant clarified composition will have a darker brown appearance.
  • a liquid composition consisting essentially of:
  • Component (A) fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and
  • Component (B) a liquid from the group consisting of water, a normally liquid, water miscible polyhydric alcohol, and both water and a. normally liquid, water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32% fermentation soluble solids by weight at 4.4"C., said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50% when said composition is stored in a container under atmospheric pressure for six days at 42C., Component (A) constituting at least 32% by weight of the solids content of said composition, on a dry basis.
  • composition as claimed in claim 1 in which the protease activity is at least 5,000 PV units per gram at 4.4C.
  • composition as claimed in claim 1 in which the protease activity is 5,000to 2,000,000 PV units per gram at 44C.
  • composition as claimed in claim 1 in which the protease activity is 30,000 to 600,000 PV units per gram at 4.4C.
  • composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32% by weight to saturation.
  • composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32 to 43.5 percent by weight.
  • component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial protease, and component (B) consists essentially of glycerol.
  • component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial amylase, and component (B) consists essentially of glycerol.
  • component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of both bacterial protease and bacterial amylase, and component (B) consists essentially of glycerol.
  • a process of clarifying an aqueous solution of an enzyme from the group consisting of bacterial protease, bacterial amylase and mixtures thereof which comprises adding to a turbid aqueous solution in which the concentration of said enzyme is at least 32 percent by weight, on a dry basis, a sufficient amount of a liquid, water miscible polyhydric alcohol to reduce the turbidity.

Abstract

Liquid concentrates of bacterial protease and/or amylase of enhanced stability are prepared. Bacterial protease and/or amylase liquid compositions free of turbidity and a process of producing them are provided. These compositions have advantages over powdered enzymes in avoiding atmospheric contamination and are especially useful in detergents and in textile treatment.

Description

ljnited States Patent 1 Zitter 51 Feb. 20, 1973 54 LIQUID COMPOSITIONS 0F 3,472,783 10/1969 Smilliemi ..195/68 x BACTERIAL PROTEASE AND/0R 3,296,094 l/l967 Cayle AMYLASE AND PREPARATION 3,095,358 6/1963 Meister ..195/63 THEREOF Inventor: Jack Zitfer, Milwaukee, Wis.
Assignee: Pabst Brewing Company, Milwaukee, Wis.
Filed: Sept. 25, 1970 Appl. No.: 75,764
US. Cl ..195/63, 195/68, 252/DIG. 12, 8/138 Int. Cl. ..C07g 7/02 Field of Search ..195/68, 63, 62, 65, 66; 252/DIG. 12
References Cited UNITED STATES PATENTS 5/1962 Smythe et al ..99/222 1/1971 McCarty ..252/89 OTHER PUBLICATIONS Yasumatsu et a1. Agricultural and Biological Chemistry vol. 29, No. 7 pp. 665-671 (1965) Vol.
Primary Examiner-Lionel M. Shapiro Attorney-Johnston, Root, OKeeffe, Keil, Thompson & Shurtleff [57] ABSTRACT 20 Claims, No Drawings LIQUID COMPOSITIONS OlF BACTERIAL PROTEASE AND/OR AMYLASE AND PREPARATION THEREOF BACKGROUND OF THE INVENTION In recent years the use of enzymes, especially bacterial protease and bacterial amylase, for presoak and detergent washing products has greatly increased. These enzymes are now being produced in the form of powders in very high potency concentrations and handling problems have developed as a result of these high potency levels. Many individuals have found these enzymes to be a source of irritation. The problem is particularly acute in the manufacturing and handling of the enzymes themselves and also in the handling of the enzymes in plants where they are formulated into various forms of detergent products. In order to alleviate the problem it has heretofore been proposed to produce a dust-free powdered enzyme product.
Another possible solution of the problem would be the provision of the enzymes in the form of a liquid composition. When attempts are made to do this, however, other problems arise. One of these problems is the instability of the enzymes which causes the enzyme activity of a liquid product to be reduced in storage. Another problem arises due to the fact that the enzymes as normally produced form turbid solutions in water containing suspended particles. It would, therefore, be desirable to solve the dusting problem which is a source of irritation to individuals by providing a liquid composition containing enzymes which retain a relatively high activity when the composition is stored prior to use. In addition, It would be desirable to provide high potency liquid concentrates of bacterial protease and/or amylase which are free of turbidity. These liquid concentrates of bacterial protease and/or amylase of enhanced stability would avoid the source of irritation caused by the dusting of powdered enzymes and they could be used in the manufacture of various detergent products. In addition, liquid enzyme compositions tree from turbidity would be acceptable for use as such in household detergent formulations and also as enzyme compositions for the treatment of textiles, for example, in the de-sizing of textile fibers.
OBJECTS One of the ob pets of the present invention is to provide new and improved liquid concentrates of bacterial protease and/or bacterial amylase of enhanced stability.
Another object of the invention is to provide liquid compositions containing bacterial protease and/or bacterial amylase which are free of turbidity.
Still a further object of the invention is to provide a new and improved process for producing liquid compositions containing bacterial protease and/or bacterial amylase.
Another ob ject of the invention is to provide new and improved liquid compositions-containing bacterial protease and/or bacterial amylase in combination with surfactants. Other objects will appear hereinafter.
BRIEF SUMMARY OF THE INVENTION In accordance with the invention liquid compositions are prepared consisting essentially of: Component (A),
fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and Component (B), a liquid from the group consisting of water, a normally liquid water miscible polyhydric alcohol, and both water and a normally liquid water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32 percent by weight fermentation soluble solids at 4.4C. (40F), said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50 percent when said composition is stored in a container under atmospheric pressure for 6 days at 42C.
The compositions of the invention will normally have a protease activity of at least 5,000 PV units per gram at 44C., preferably within the range of 5,000 to 2,000,000 PV units per gram at 44C., and generally within the range of 30,000 to 600,000 PV units per gram at 4.4C. The amylase activity can be up to 1,000,000 DV units per gram at 4.4C., is preferably at least 10,000 DV units per gram at 44C., and is generally within the range of 30,000 to 400,000 DV units per gram at 4.4C. The enzymes in the liquid compositions can consist essentially of bacterial protease or bacterial amylase or both bacterial protease and bacterial amylase in the above described proportions.
The liquid compositions can be used as such for washing or they can be mixed with other ingredients for the preparation of detergent compositions. Thus, they can be mixed with various types of compatible surfactants to form liquid concentrates which can be added to wash water in predetermined amounts.
It has been found that where Component (B) is water, the stability of the enzymes is enhanced by maintaining a relatively high enzyme concentration, namely, a concentration of at least 32 percent by weight at 4.4C. (40F). Where a polyhydric alcohol such as glycerol, either alone or mixed with water, constitutes the liquid phase, the retained activity of the enzymes over a period of time is enhanced even at enzyme concentrations below 32 percent by weight. Furthermore, polyhydric alcohols, for example, glycerol, propylene glycol, ethylene glycol, diethylene glycol and higher liquid homologues, have the property of removing the turbidity which is normally present in concentrated bacterial protease and bacterial amylase solutions in water. Hence, the use of polyhydric alcohols in the preparation of these liquid compositions makes it possible to produce clear liquid compositions which do not leave deposits when employed in washing procedures and in textile treatment and are therefore more acceptable for such uses.
DETAILED DESCRIPTION OF THE INVENTION In preparing compositions in accordance with the invention several different methods may be used. The usual procedure involves first preparing an aqueous concentrate by adding bacterial protease and bacterial amylase to water and agitating the resultant composition at room temperature (2032C.) so as to dissolve the enzyme. The composition is then filtered and the filter cake washed with water. The combined filtrate and wash water after concentration is a turbid solution, probably containing colloidal protein solid in suspension. In accordance with the invention it has been found that the retained enzyme activity of this solution on standing is enhanced by increasing the proportion of enzyme solids in the solution to the point where they are at least 32 percent by weight, based on the weight of the dry solids, and preferably within the range of 32 percent to saturation. In most cases this range will be from 32 to 45 percent by weight.
Surprisingly, when a liquid polyhydric alcohol is added to the previously described turbid enzyme solution it clarifies the solution. Furthermore, when the polyhydric alcohol is glycerol, the enzyme solution is not only clarified but the retained enzyme activity on storage is greatly enhanced, even when the concentration of enzyme solids is less than 32 percent by weight. Thus, the concentration of enzyme solids can be within the range of 2 to 60 percent by weight and is usually within the range of 32 to 43.5 percent by weight. Where glycerol is employed the composition may contain 40 to 98 percent by weight glycerol. Various types of compositions in which the liquid phase consists essentially of both water and a polyhydric alcohol can be prepared as hereinafter described.
As previously indicated, these liquid enzyme compositions can be employed as such in washing by adding them to the wash water in the proper proportions. In most cases the proportions should be sufficient to give an enzyme concentration of ZOO-2,000 PV units/liter and -500 DV units/liter in a water having a pH within the range of 8 to 12. Compatible surfactants can also be added to the liquid enzyme concentrates. For most purposes the surfactant is either a nonionic or an anionic surfactant. It may also be an amphoteric surfactant. Any of the compatible surfactants described in Me- Cutcheons Detergents and Emulsifiers, 1969 Annual, can be employed. Examples of such surfactants are Alrosperse DC, Triton QS-l5, QS-30, 136-5, and X-102. It is usually preferable to carry out routine experiments in order to determine the compatibility of a particular surfactant. Some of these surfactants are compatible with some polyhydric alcohols but not with others.
The invention will be further illustrated but is not limited by the following examples in which the quantities are stated in parts by weight unless otherwise specified.
EXAMPLE 1 A 20 percent (w/v) solution of Al (SO 'l8H- O was slowly added to an agitated bacterial protease fermentation whole culture (500 gal, 10,720 PV units per ml., 20.3 X PV units) while maintaining the whole culture pH at 6.4 by the simultaneous addition of Na SO (42.5 lb.). The amount of Al (SO.,) -l8l-I O added was 1 percent'(w/v) based on the whole culture. Coagulants Nalco 674 (120 grams, as a 0.25 percent (w/v) solution) and Purifloc C-31(5,025 grams, as a 27 percent (w/v) solution were then added, together with filteraid Chem-Flo 325 CK (290 lbs.). The mixture (pH 5.3) was adjusted to pH 6.4 with NaOH and filtered on a plate and frame press. The resultant filtered cake was washed with water and the combined clear solution of filtrate plus wash evaporated-in vacuo to 83.6 gal. (pl-l 6.3, 18.1 X 10 PV units) for a filtration and concentration recovery of 89.2 percent. Sodium sulfate (7,000 grams), sodium sulfite (1,600 grams) and sodium bisulfite (200 grams) were added to a portion of the concentrate warmed to 45C. (40,000 ml., pl-l6.4, 25 percent dry solids, 22.40 X 10 PV units) and the mixture filtered on a small Dorr-Oliver rotary filter using a Na SO wash. The total PV units recovered in the filtered cake (4,794 grams) and heel totaled 21.94 X 10 PV units for a filtration recovery of 97.9 percent. The overall recovery from the whole culture was 86.7 percent.
EXAMPLE 2 The bacterial protease filtered cake obtained in Example 1 after precipitation with Na SO Na SO and Nal-ISO was used to prepare a series of aqueous concentrates. Distilled water (7,200 ml.) was added to a portion of the filtered cake (1,765 grams, 680 X 10 PV units) and the mixture agitated at room temperature for 1.5 hours. The mixture was then filtered on a Buchner funnel using a Filteraid FW-20 pre-coat and the filtered cake washed with water (3,000 ml.). The combined filtrate and washed totaled 11,840 ml. and contained 620 X 10 PV units, for a protease enzyme recovery of 91.2 percent. Portions of the combined filtrate and wash were evaporated in vacuo to varying concentrations of dry solids. The resultant concentrates werevisually tan turbid solutions, appearing to contain colloidal protein solid in suspension. Aliquots of these concentrates were tested at 4.4C. (40F.) and 42C. (107.6F.) for storage stability in containers at atmospheric pressure. The results obtained are shown below.
Using the procedures described in Examples 1 and 2, an aqueous concentrate was prepared having a per cent dry solids of 32 percent and a protease enzyme activity of 360,000 PV units per gram. The resultant concentrate was a tan turbid solution containing suspended solid.
A portion of the aqueous concentrate grams) was added to distilled water (20 grams). The resultant mixture was still a tan turbid solution containing suspended solid. Aliquots of this solution (without any further evaporation) were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (100 grams) was added to propylene glycol (20 grams, reagent grade) resulting in an almost complete clearing of the turbidity of the aqueous concentrate. Aliquots of this solution (without any further evaporation) were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (100 grams) was added to propylene glycol (50 grams, reagent grade) and the mixture evaporated in vacuo to 100 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (100 grams) was added to propylene glycol (50 grams, reagent grade) and the mixture evaporated in vacuo to 100 grams. An additional 20 grams of propylene glycol was then added to the clear solution resulting in a final total weight of 120 grams, of which 70 grams constituted propylene glycol. Aliquots of this clear solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (200 grams) was added to ethylene glycol (100 grams, reagent grade) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
Additional ethylene glycol (20 grams) was added to a portion of the above ethylene glycol-aqueous solution (100 grams). Aliquots of this clear mixture (without any further concentration) were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (200 grams) was added to diethylene glycol (100 grams) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
Additional diethylene glycol (20 grams) was added to a portion of the above diethylene glycol-aqueous solution (100 grams). Aliquots of this clear mixture (without any further concentration were tested at 4.4C. and 42C for storage stability.
A portion of the aqueous concentrate (200 grams) was added to glycerol (100 grams, U.S.P. grade) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
Additional glycerol (20 grams) was added to a portion of the above glycerolaqueous solution (100 grams). Aliquots of this clear mixture (without any further concentration) were tested at 4.4C. and 42C. for storage stability.
The data obtained for these products are as follows:
Protease activity PV/Gm. remaining after storage at indicated temperatures Poly- 96 Poly- Non- Protease hydride hydric Alcohol Activity alcohol Alcohol in Dry Storage, Remainused Product Solids Days 4.4C.42C. ing
None None 32 14 350 K 192 K 54.9
None None 26.7 I4 304 K 96 K 31.6
propylene glycol 16.7 26.7 7 320 K 200 K 62.5
propylene glycol 50 32 14 380 K 140.8 K 37.1
propylene glycol 58 26.7 7 282 K 125 K 44.3
ethylene glycol 50 32 I4 375 K 125.4 k 33.4
ethylene glycol 58 26.7 7 275 k 95 it 34.5
diethylene glycol 50 32 13 375 k 98.4 k 26.2
diethylene glycol 58 26.7 6 282.5 k-207.5 k 73.5
glycerol 50 32 6 340 k 325 it 95.6 21 340 k 315 k 92.6 43 355 k 286.4 k 80.7
glycerol 58 26.7 6 302.5k 275 k 90.9 21 300 k 252 84.0 43 310 k 280 .3 k 90.4
EXAMPLE 4 Using the procedures described in Examples 1 and 2, an aqueous protease concentrate was prepared having a per cent dry solids of 38.3 percent and a protease enzyme activity of 355,000 PV units per gram. The resultant concentrate was a tan turbid solution containing suspended solid. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (200 grams) was added to glycerol grams, U.S.P. grade) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
The data obtained for these products are shown below:
Using the procedures described in Examples 1 and 2, an aqueous concentrate was prepared having a per cent dry solids of 32 percent and a protease enzyme activity of 350,000 PV units per gram. The resultant concentrate was a tan turbid solution containing suspended solid.
A portion of the aqueous concentrate (400 grams) was added to glycerol (128 grams, U.S.P. grade) and the mixture evaporated in vacuo to 294 grams. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (100 grams) was added to glycerol (55 grams, U.S.P. grade) and the mixture evaporated in vacuo to 100 grams. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (100 grams) was added to glycerol (60 grams, U.S.P. grade) and the mixture evaporated in vacuo to 100 grams. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate grams) was added to glycerol (53 grams, U.S.P. grade) and the mixture evaporated in vacuo to 107 grams. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (120 grams) was added to glycerol (48 grams, U.S.P. grade) and the mixture evaporated in vacuo to 98 grams. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
The data obtained for these products are shown below:
Protease Activity PV/Grn. Remaining After Storage At indicated Temperatures Protease nonactivity glycerol glycerol storage, remainin product Dry solids days 4.4C. 42C. ing
EXAMPLE 6 Using the procedures described in Examples 1 and 2, an aqueous concentrate was prepared having a percent dry solids of 38.9 percent and a protease enzyme activity of 381,000 PV units per gram. The resultant product was a tan turbid solution containing suspended solids. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
A portion of the aqueous concentrate (1,000 grams) was added to glycerol (500 grams, U.S.P. grade) and the mixture evaporated in vacuo to 1,000 grams. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 42C. for storage stability.
Portions of the glycerol-aqueous concentrate were then further diluted with additional glycerol in the glycerol concentration range of 50-99 percent of the total solution. The resultant products were clear solutions. Aliquots of these solutions were tested at 4.4C. and 42C. for storage stability.
The data obtained for these products are shown below:
Protease Activity PV/Gm.
Using the salt treatment and precipitation procedure described in U.S. Ser. No. 849,148, filed Aug. 1 l, 1969, a filtered precipitated bacterial amylase wet cake was obtained assaying 128,000 DV units per gram. Tap water (4,000 ml.) was added to a portion of the filtered cake (1,000 grams, 128.0 X DV units) and the mixture agitated at room temperature for one hour.
Al (SO.,) l8l-l O (25 grams, as a 20 percent (w/v) solution) was then added and the pH maintained at 6.4 by the simultaneous addition of N21 80:, (30 grams). After the addition of the salts, Chem-Flo 325 CK fi1 teraid (350 grams) was added and the mixture filtered on a Buchner funnel, followed by a tap water wash. The combined filtrate and wash (7,850 rnl., pH 6.65) was evaporated in vacuo to a concentrate (33.6 percent dry solids, pH 6.3) containing 121.1 X 10 DV units for an amylase enzyme recovery of 94.6 percent. The resultant concentrate was a visually tan turbid solution, appearing to contain colloidal protein solid in suspension. Aliquots of this concentrate were tested at 4.4C., 28C. and 42C. for storage stability. The results obtained are shown below:
Amylase Activity (DV/Gm.) After 36 Days at Indicated Temperature Dry Solids ln Concentrate 4.4C. 42C.
EXAMPLE 8 a. Using the procedures described in Example 3, a portion of the aqueous concentrate (200 grams) from Example 7 was added to glycerol grams, U.S.P. grade) and the mixture evaporated in vacuo to 200 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C., 28C., 42C. and 55C. for storage stability. The results obtained are shown below:
Amylase Activity (DV/Gm.) After non-glycerol 30 Days at Indicated glycerol dry Temperature in concentrate solids 4.4C. 28C. 42C. 55C.
b. Using the procedures described in Example 3, a portion of the aqueous concentrate (450 grams) from Example 7 was added to glycerol (225 grams, U.S.P. grade) and the mixture evaporated in vacuo to 450 grams final weight. The resultant product was a clear solution. Aliquots of this solution were tested at 4.4C. and 55C. for storage stability. The results obtained are An enzyme concentrate was prepared by mixing equal parts by weight of a bacterial protease-glycerol concentrate containing 50 percent glycerol by weight and having an activity of 464,000 PV units per gram with a bacterial amylase concentrate containing 50 percent glycerol by weight and having an activity of 90,680 DV units per gram and 20,000 PV units per gram.
The final product contained a protease concentration of 242,000 PV units per gram and 45,340 DV units per gram.
EXAMPLE 10 The procedure was the same as in Example 9 except that additional glycerol was added and the relative proportions of bacterial protease-glycerol concentrate, bacterial amylase-glycerol concentrate and glycerol were, respectively, 12.42 percent, 65.70 percent and 21.88 percent by weight. The final product had a protease concentration of 70,760 PV units per gram and 59,5 80 DV units per gram.
EXAMPLE 1 l The procedure was the same as in Example 9 except that additional glycerol was added and the relative proportions of bacterial protease-glycerol concentrate, bacterial amylase-glycerol concentrate and glycerol were 46.25 percent, 37.05 percent and 16.70 percent by weight, respectively. The final product contained a protease concentration of 222,200 PV units per gram and 33,600 DV units per gram.
The following examples illustrate the preparation of enzyme compositions of the type described containing surfactants.
EXAMPLE 12 The bacterial protease-glycerol concentrate of Example 9 containing 50 percent by weight glycerol and having a protease concentration of 464,000 PV units per gram was mixed in a weight ratio of 4:1 with Alrospere DC, to produce a composition having a protease concentration of 371,200 PV units per gram.
EXAMPLE 13 The procedure was the same as in Example 12 except that 80 parts of the protease-glycerol concentrate was mixed with 5 parts Alrosperse DC and parts of additional glycerol. The protease concentration was the same as in Example 12.
EXAMPLE 14 The procedure was the same as in Example 12 except that the surfactant Triton 08-1 5 was used.
EXAMPLE 15 The procedure was the same as in Example 13 except that the surfactant Triton 08-15 was used.
' EXAMPLE 16 The procedure was the same as in Example 12 except that the surfactant Triton BG-5 was used.
EXAMPLE 17 The procedure was the same as in Example 13 except that the surfactant Triton BG-S was used.
EXAMPLE 18 The procedure was the same as in Example 12 except that a bacterial amylase-glycerol concentrate containing 50 percent by weight glycerol was used instead of the bacterial protease-glycerol concentrate. Since the bacterial amylase contained some protease, the final product had an amylase concentration of 72,540 DV units per gram and a protease concentration of 16,000 PV units per gram.
EXAMPLE 19 The procedure was the same as in Example 13 except that a bacterial amylase-glycerol concentrate of the type employed in Example 18 was used. The final product had an amylase concentration of 72,540 DV units per gram and a protease concentration of 16,000 PV units per gram.
EXAMPLE 20 The procedure was the same as in Example 18 except that Triton QS-l 5 was used instead of Alrosperse DC.
EXAMPLE 21 The procedure was the same as in Example 19 except that Triton 08-15 was used instead of Alrosperse DC.
EXAMPLE 22 The procedure was the same as in Example 18 except that Triton BG-S was used instead of Alrosperse DC.
EXAMPLE 23 The procedure was the same as in Example 19 except that Triton BG-S was used instead of Alrosperse DC.
EXAMPLE 24 per gram.
EXAMPLE 25 The procedure was the same as in Example 24 except that Triton BG-S was substituted for the Alrosperse DC.
EXAMPLE 26 The procedure was the same as in Example 24 except that Triton QS-15 was substituted for the Alrosperse DC.
It should be noted that the bacterial amylase enzyme normally contains some bacterial protease enzyme. in Example 7 the protease enzyme activity is not given.
In Examples 12 to 26 all of the surfaces were found to be compatible with the enzyme concentrates. The Alrosperse DC is an oil soluble surfactant which is nonionic plus a cationic mixture of fatty alkoamides. Triton BG-S is a non-ionic surfactant. Triton QS-l 5 is a liquid anhydrous amphoteric surfactant in the form of an oxyethylated sodium salt containing both anionic and cationic centers. All of these surfactants are identified in McCutcheons 1969 Annual, supra.
In the foregoing examples the recovery procedures used in preparing bacterial protease and/or the bacterial amylase, as illustrated specificallyin Example 1, do not constitute a part of the present invention but are disclosed and claimed in US. application Ser. No. 849,148 filed Aug. 11, 1969.
It may be mentioned that the clarity of the enzyme solution depends upon the particular enzyme and the concentration thereof. The addition of the polyhydric alcohol serves to reduce the turbidity and thereby increase the clarity of the solution. This does not necessarily mean, however, that one can see through a given sample of solution after it has been clarified. Thus, in the case of enzyme compositions which are concentrated to the point that they are of a syrupy consistency, the composition prior to treatment with the polyhydric alcohol will contain suspended particles which cause turbidity and will usually have a tan appearance whereas after the treatment with a polyhydric alcohol the turbidity will be reduced due to the dissolution of the particles and the resultant clarified composition will have a darker brown appearance.
The invention is hereby claimed as follows:
1. A liquid composition consisting essentially of:
Component (A), fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and
Component (B), a liquid from the group consisting of water, a normally liquid, water miscible polyhydric alcohol, and both water and a. normally liquid, water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32% fermentation soluble solids by weight at 4.4"C., said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50% when said composition is stored in a container under atmospheric pressure for six days at 42C., Component (A) constituting at least 32% by weight of the solids content of said composition, on a dry basis.
2. A composition as claimed in claim 1 in which the protease activity is at least 5,000 PV units per gram at 4.4C.
3. A composition as claimed in claim 1 in which the protease activity is 5,000to 2,000,000 PV units per gram at 44C.
4. A composition as claimed in claim 1 in which the protease activity is 30,000 to 600,000 PV units per gram at 4.4C.
5. A composition as claimed in claim 1 in which the amylase activity is at least l0,000 DV units per gram at 4.4C.
6. A composition as claimed in claim 1 in which the amylase activity is 10,000 to 1,000,000 DV units per gram at 4.4C.
7. A composition as claimed in claim 1 in which the amylase activity is 30,000 to 400,000 DV units per gram at 4.4C.
8. A composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32% by weight to saturation.
' 9. A composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32 to 43.5 percent by weight.
10. A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial protease, and component (B) consists essentially of glycerol.
1 A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial amylase, and component (B) consists essentially of glycerol.
12. A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of both bacterial protease and bacterial amylase, and component (B) consists essentially of glycerol.
13. A composition as claimed in claim 1 in which said enzyme is present in a concentration of at least 32 percent by weight, on a dry basis, and said liquid is from the group consisting of glycerol and water.
14. A process of clarifying an aqueous solution of an enzyme from the group consisting of bacterial protease, bacterial amylase and mixtures thereof which comprises adding to a turbid aqueous solution in which the concentration of said enzyme is at least 32 percent by weight, on a dry basis, a sufficient amount of a liquid, water miscible polyhydric alcohol to reduce the turbidity.
15. A process as claimed in claim 14 in which said polyhydric alcohol is glycerol.
16. A process as claimed in claim 14 in which said enzyme is bacterial protease.
17. A process as claimed in claim 14 in which said aqueous solution containing said polyhydric alcohol is concentrated under conditions facilitating the elimination of at least a part of the water without removing said polyhydric alcohol.
18. A process as claimed in claim 17 in which said concentration is carried out under subatmospheric pressure conditions.
19. A process as claimed in claim 14 in which said enzyme is bacterial amylase.
20. A process as claimed in claim 14 in which said enzyme is a mixture of bacterial protease and bacterial amylase.

Claims (19)

1. A liquid composition consisting essentially of: Component (A), fermentation soluble solids consisting essentially of an enzyme from the group consisting of bacterial protease and bacterial amylase and mixtures thereof, and Component (B), a liquid from the group consisting of water, a normally liquid, water miscible polyhydric alcohol, and both water aNd a normally liquid, water miscible polyhydric alcohol, in which said enzyme is soluble to the extent of at least 32% fermentation soluble solids by weight at 4.4*C., said composition containing a concentration of said components sufficient to maintain an enzyme activity of at least 50% when said composition is stored in a container under atmospheric pressure for six days at 42*C., Component (A) constituting at least 32% by weight of the solids content of said composition, on a dry basis.
2. A composition as claimed in claim 1 in which the protease activity is at least 5,000 PV units per gram at 4.4*C.
3. A composition as claimed in claim 1 in which the protease activity is 5,000 to 2,000,000 PV units per gram at 4.4*C.
4. A composition as claimed in claim 1 in which the protease activity is 30,000 to 600,000 PV units per gram at 4.4*C.
5. A composition as claimed in claim 1 in which the amylase activity is at least 10,000 DV units per gram at 4.4*C.
6. A composition as claimed in claim 1 in which the amylase activity is 10,000 to 1,000,000 DV units per gram at 4.4*C.
7. A composition as claimed in claim 1 in which the amylase activity is 30,000 to 400,000 DV units per gram at 4.4*C.
8. A composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32% by weight to saturation.
9. A composition as claimed in claim 1 in which the solids content, on a dry basis, of the enzyme is within the range of 32 to 43.5 percent by weight.
10. A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial protease, and component (B) consists essentially of glycerol.
11. A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of bacterial amylase, and component (B) consists essentially of glycerol.
12. A composition as claimed in claim 1 in which component (A) consists essentially of at least 32 percent by weight, on a dry basis, of the composition of both bacterial protease and bacterial amylase, and component (B) consists essentially of glycerol.
13. A composition as claimed in claim 1 in which said enzyme is present in a concentration of at least 32 percent by weight, on a dry basis, and said liquid is from the group consisting of glycerol and water.
14. A process of clarifying an aqueous solution of an enzyme from the group consisting of bacterial protease, bacterial amylase and mixtures thereof which comprises adding to a turbid aqueous solution in which the concentration of said enzyme is at least 32 percent by weight, on a dry basis, a sufficient amount of a liquid, water miscible polyhydric alcohol to reduce the turbidity.
15. A process as claimed in claim 14 in which said polyhydric alcohol is glycerol.
16. A process as claimed in claim 14 in which said enzyme is bacterial protease.
17. A process as claimed in claim 14 in which said aqueous solution containing said polyhydric alcohol is concentrated under conditions facilitating the elimination of at least a part of the water without removing said polyhydric alcohol.
18. A process as claimed in claim 17 in which said concentration is carried out under subatmospheric pressure conditions.
19. A process as claimed in claim 14 in which said enzyme is bacterial amylase.
US00075764A 1970-09-25 1970-09-25 Liquid compositions of bacterial protease and/or amylase and preparation thereof Expired - Lifetime US3717550A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7576470A 1970-09-25 1970-09-25

Publications (1)

Publication Number Publication Date
US3717550A true US3717550A (en) 1973-02-20

Family

ID=22127829

Family Applications (1)

Application Number Title Priority Date Filing Date
US00075764A Expired - Lifetime US3717550A (en) 1970-09-25 1970-09-25 Liquid compositions of bacterial protease and/or amylase and preparation thereof

Country Status (1)

Country Link
US (1) US3717550A (en)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3963578A (en) * 1974-12-20 1976-06-15 E. I. Du Pont De Nemours And Company Stabilization of phosphoenol pyruvate carboxylase
US4162987A (en) * 1976-06-24 1979-07-31 The Procter & Gamble Company Enzyme-containing automatic dishwashing detergent composition
US4238345A (en) * 1978-05-22 1980-12-09 Economics Laboratory, Inc. Stabilized liquid enzyme-containing detergent compositions
US4391745A (en) * 1979-03-09 1983-07-05 Diamalt Aktiengesellschaft Desizing agent and process for preparation thereof
DE3344097A1 (en) * 1982-12-13 1984-06-14 Colgate-Palmolive Co., New York, N.Y. ENZYME-CONTAINING LIQUID DETERGENT AND CLEANING AGENT
US4497897A (en) * 1982-12-09 1985-02-05 Novo Industri A/S Liquid proteinase concentrate and method for preparation
US4511490A (en) * 1983-06-27 1985-04-16 The Clorox Company Cooperative enzymes comprising alkaline or mixtures of alkaline and neutral proteases without stabilizers
US4566985A (en) * 1984-09-19 1986-01-28 Applied Biochemists, Inc. Method of cleaning using liquid compositions comprising stabilized mixtures of enzymes
US4673647A (en) * 1985-05-06 1987-06-16 Miles Laboratories, Inc. Process to solubilize enzymes and an enzyme liquid product produced thereby
US4801544A (en) * 1984-09-12 1989-01-31 The Clorox Company Method of improving the storage life of liquid compositions containing enzymes
JPH01180515A (en) * 1988-01-13 1989-07-18 Tome Sangyo Kk Cleaning liquid and cleaning method for contact lens
US5269960A (en) * 1988-09-25 1993-12-14 The Clorox Company Stable liquid aqueous enzyme detergent
WO1996017632A1 (en) * 1994-12-06 1996-06-13 Betzdearborn Inc. Biofilm avoidance and removal process and composition
US5531898A (en) * 1995-04-06 1996-07-02 International Organic Solutions Corp. Sewage and contamination remediation and materials for effecting same
US5558812A (en) * 1993-06-16 1996-09-24 Solvay Enzymes Gmbh & Co. Kg. Liquid enzyme formulations
US5789239A (en) * 1995-06-05 1998-08-04 Betzdearborn Inc. Composition and process for the avoidance of slime formation and/or for the removal of biofilm in water-bearing systems
US20030165487A1 (en) * 2002-02-12 2003-09-04 Juan Tricarico Amylase feed supplements for improved ruminant nutrition

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3033691A (en) * 1959-08-26 1962-05-08 Rohm & Haas Meat tenderizing compositions
US3095358A (en) * 1961-03-15 1963-06-25 Baxter Laboratories Inc Liquid chill proofing composition
US3296094A (en) * 1966-05-05 1967-01-03 Baxter Laboratories Inc Stabilized aqueous enzyme solutions
US3472783A (en) * 1966-02-02 1969-10-14 Winston B Smillie Nonionic detergent compositions
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3033691A (en) * 1959-08-26 1962-05-08 Rohm & Haas Meat tenderizing compositions
US3095358A (en) * 1961-03-15 1963-06-25 Baxter Laboratories Inc Liquid chill proofing composition
US3472783A (en) * 1966-02-02 1969-10-14 Winston B Smillie Nonionic detergent compositions
US3296094A (en) * 1966-05-05 1967-01-03 Baxter Laboratories Inc Stabilized aqueous enzyme solutions
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Yasumatsu et al. Agricultural and Biological Chemistry vol. 29, No. 7 pp. 665 671 (1965) Vol. *

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3963578A (en) * 1974-12-20 1976-06-15 E. I. Du Pont De Nemours And Company Stabilization of phosphoenol pyruvate carboxylase
US4162987A (en) * 1976-06-24 1979-07-31 The Procter & Gamble Company Enzyme-containing automatic dishwashing detergent composition
US4238345A (en) * 1978-05-22 1980-12-09 Economics Laboratory, Inc. Stabilized liquid enzyme-containing detergent compositions
US4391745A (en) * 1979-03-09 1983-07-05 Diamalt Aktiengesellschaft Desizing agent and process for preparation thereof
US4497897A (en) * 1982-12-09 1985-02-05 Novo Industri A/S Liquid proteinase concentrate and method for preparation
DE3344097A1 (en) * 1982-12-13 1984-06-14 Colgate-Palmolive Co., New York, N.Y. ENZYME-CONTAINING LIQUID DETERGENT AND CLEANING AGENT
FR2537597A1 (en) * 1982-12-13 1984-06-15 Colgate Palmolive Co LIQUID DETERGENT COMPOSITION CONTAINING ENZYME MIXTURE AND METHOD FOR WASHING THE LAUNDRY USING THE SAME
US4511490A (en) * 1983-06-27 1985-04-16 The Clorox Company Cooperative enzymes comprising alkaline or mixtures of alkaline and neutral proteases without stabilizers
US4801544A (en) * 1984-09-12 1989-01-31 The Clorox Company Method of improving the storage life of liquid compositions containing enzymes
US4566985A (en) * 1984-09-19 1986-01-28 Applied Biochemists, Inc. Method of cleaning using liquid compositions comprising stabilized mixtures of enzymes
US4673647A (en) * 1985-05-06 1987-06-16 Miles Laboratories, Inc. Process to solubilize enzymes and an enzyme liquid product produced thereby
JPH01180515A (en) * 1988-01-13 1989-07-18 Tome Sangyo Kk Cleaning liquid and cleaning method for contact lens
JPH0577047B2 (en) * 1988-01-13 1993-10-25 Tome Sangyo Kk
US5269960A (en) * 1988-09-25 1993-12-14 The Clorox Company Stable liquid aqueous enzyme detergent
US5558812A (en) * 1993-06-16 1996-09-24 Solvay Enzymes Gmbh & Co. Kg. Liquid enzyme formulations
AU695600B2 (en) * 1994-12-06 1998-08-20 Hercules Incorporated Biofilm avoidance and removal process and composition
WO1996017632A1 (en) * 1994-12-06 1996-06-13 Betzdearborn Inc. Biofilm avoidance and removal process and composition
EP0796114A1 (en) * 1994-12-06 1997-09-24 BetzDearborn Inc Biofilm avoidance and removal process and composition
EP0796114A4 (en) * 1994-12-06 2000-06-14 Betzdearborn Inc Biofilm avoidance and removal process and composition
US5531898A (en) * 1995-04-06 1996-07-02 International Organic Solutions Corp. Sewage and contamination remediation and materials for effecting same
US5789239A (en) * 1995-06-05 1998-08-04 Betzdearborn Inc. Composition and process for the avoidance of slime formation and/or for the removal of biofilm in water-bearing systems
US20030165487A1 (en) * 2002-02-12 2003-09-04 Juan Tricarico Amylase feed supplements for improved ruminant nutrition

Similar Documents

Publication Publication Date Title
US3717550A (en) Liquid compositions of bacterial protease and/or amylase and preparation thereof
DE3785551T3 (en) Detergents for clothes.
US3700561A (en) Recovery of enzymes
Skeggs Jr et al. The purification of hypertensin II
EP0171007B1 (en) Dish cleaning agent
DE69433032T2 (en) LIQUIDIFYING - [alpha] -AMYLASE, METHOD FOR THE PRODUCTION THEREOF, AND DETERGENT COMPOSITION CONTAINING THE SAME
DE2551742C3 (en) Protease preparation and its use in detergents
US5271860A (en) Bleaching liquid detergent
US3860536A (en) Enzyme-detergent combination
DE3117250A1 (en) "MAIN DETERGENT, WASHING METHOD AND SUITABLE TEXTILE SOFTENER THEREFOR"
DK158233B (en) Protease preparation, process for preparing it and use thereof
DE2224777A1 (en) NEW ALKALINE PROTEOLYTIC ENZYME, ITS PRODUCTION AND USE
EP0482046B1 (en) Enzymatic cleaner
US3960665A (en) Production of proteolytic enzymes
EP0205999B1 (en) Laundry soil and stain remover
DE1906001C3 (en) Alkali proteases and detergents containing them
US3574120A (en) Highly alkaline detergent composition containing an enzyme derived from thermophilic streptomyces rectus var. proteolyticus
CN111304015A (en) Antibacterial high-concentration laundry detergent and preparation method thereof
US4497897A (en) Liquid proteinase concentrate and method for preparation
CN1443234A (en) Direct enzyme agglomeration process
EP0171006B1 (en) Dish cleaning agent
DE3834550A1 (en) PROTEOLYTIC ENZYME, ITS MANUFACTURE AND USE
US2926122A (en) Method of preparing glucose oxidase
US5759984A (en) Enzyme stabilization
DE1943694A1 (en) Washing agent contg anti-microbial substances

Legal Events

Date Code Title Description
AS Assignment

Owner name: LAWYERS TITLE COMPANY OF WASHINGTON, INC. (TRUSTEE

Free format text: SECURITY INTEREST;ASSIGNOR:PABST BREWING COMPANY A DE CORP.;REEL/FRAME:004129/0649

Effective date: 19830321

Owner name: FIRST WISCONSIN NATIONAL BANK OF MILWAUKEE, A NATI

Free format text: SECURITY INTEREST;ASSIGNOR:PABST BREWING COMPANY A DE CORP.;REEL/FRAME:004129/0649

Effective date: 19830321

AS Assignment

Owner name: PMP ACQUISITION CORPORATION, A DE CORP.

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:PAPST BREWING COMPANY;REEL/FRAME:004390/0892

Effective date: 19841231

STCF Information on status: patent grant

Free format text: PATENTED FILE - (OLD CASE ADDED FOR FILE TRACKING PURPOSES)