US3902972A - Modified petri dish for automatic inoculation - Google Patents

Modified petri dish for automatic inoculation Download PDF

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Publication number
US3902972A
US3902972A US469458A US46945874A US3902972A US 3902972 A US3902972 A US 3902972A US 469458 A US469458 A US 469458A US 46945874 A US46945874 A US 46945874A US 3902972 A US3902972 A US 3902972A
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Prior art keywords
media
tray
compartments
ribs
bottom wall
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US469458A
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Orville A Beckford
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Corning Glass Works
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Corning Glass Works
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Assigned to FIRST CITY, TEXAS-HOUSTON, N.A., A NATIONAL BANKING ASSOCIATION reassignment FIRST CITY, TEXAS-HOUSTON, N.A., A NATIONAL BANKING ASSOCIATION SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FLOW LABORATORIES, INC., A MD CORP.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples

Definitions

  • a dish 18 provided for use in mechanical inoculation devices employing an inoculating loop or cotton swab.
  • the tray has compartments containing combinations [21] APPL NO; 469,458 of differential or selective media to separate mixtures of gram-positive and gram-negative bacteria, facilitate Related pp Data isolation of organisms from clinical specimens and [63] Continuation-impart of Ser. No. 329,084, Feb. 2, compare colony growth characteristics of pure cul- 1973, abandoned. tures.
  • the tray has compartments to separate the media and the media is filled above the heights of the [52] U.S. CI. 195/139 barriers so that the swab or inoculating loop may [51] Int.
  • a multi-compartment dish having ribs separating the compartments.
  • Liquid media is filled slightly above the ribs and to gell in that condition whereby an inoculating tool may traverse from compartment to compartment without interference.
  • Means are provided to engage the gelled media to prevent shifting in the event of shrinkage during gelling or upon drying in storage.
  • FIG. 1 is a top plan view of the tray with its cover removed;
  • FIG. 2 is a section taken along lines 2-2 of FIG. 1.
  • the apparatus consists of a tray approximately l5 cm. by 7 cm. wide and about 4 cm. high.
  • the outer walls 12 of the tray are preferably indented, as shown at 14 to receive a cover member 16.
  • Partitions l8 and end barrier 19 extend about half the height, forming compartments 20a to 20d which contain media 22a to 22e.
  • the media uses agar as a gelling material. Because of its cohesive characteristic it forms a meniscus providing a raised ridge 23.
  • compartment 20a a specimen is swabbed across a media contained in compartment 20a using a loop or cotton swab.
  • compartments 20b, 20c 2011 and 20e containing any desired combination of selective or differential media such as gram-positive and gram-negative growth and inhibiting medias, blood agar, etc.
  • a sterile loop L is then actuated by a mechanical drive D and is then driven along path P to spread the culture along the media in a diluted fashion.
  • One advantage of automatic inoculation is that it avoids differences in technique between technicians providing greater uniformity. Further, the technician may perform other duties during inoculation.
  • a conventional inoculating loop or a cotton swab may be employed for uniform inoculation of agar media and colony isolation and combinations of media may be employed in one tray to isolate or differentiate between bacterial types. Further, this technique permits visualization of the overall qualitative, and to some extent, the quantitative relationships of the various bacterial types in the sample tested with uniformity.
  • the media has a tendency to shrink after filling and to stabilize the media pins 24 are provided which are integrally molded.
  • the pins capture the solidified gelled media and prevent shifting.
  • the stabilizing means may be ribs, cleats or otherwise shaped protruding element.
  • bacteria growth supporting media contained within said compartments, at least a portion of the upper surface of said media extending above the uppermost portion of said ribs whereby automatically driven inoculating means may freely pass from the media in one compartment to the media in adjacent compartments;
  • c. means extending from said bottom wall and partially extending into said media for immobilizing said media.
  • the tray of claim 1 including wall members surrounding the outer ones of said ribs, said wall members being higher than said ribs.
  • the tray of claim 2 including a removable cover adapted to fit over said wall members.
  • the tray of claim 1 including a plurality of first parallel compartments and a further compartment transverse to the said parallel compartments.

Abstract

A dish is provided for use in mechanical inoculation devices employing an inoculating loop or cotton swab. The tray has compartments containing combinations of differential or selective media to separate mixtures of gram-positive and gram-negative bacteria, facilitate isolation of organisms from clinical specimens and compare colony growth characteristics of pure cultures. The tray has compartments to separate the media and the media is filled above the heights of the barriers, so that the swab or inoculating loop may freely pass from one media to another. The aforementioned Abstract is neither intended to define the invention of the application which, of course, is measured by the claims, nor is it intended to be limiting as to the scope of the invention in any way.

Description

Sept. 2, 1975 MODIFIED PETRI DISH FOR AUTOMATIC INOCULATION Primary E.\'aminer-A. Louis Monacell Assistant E.\'aminerRobert J. Warden [75] Inventor: Orville A. Beckford Port Attorney, Agent, or Ftrm-Leonard H. King Washington, NY. [57] ABSTRACT [73] Assignee: Corning Glass Works, Corning,
A dish 18 provided for use in mechanical inoculation devices employing an inoculating loop or cotton swab. Filedi y 13, 1974 The tray has compartments containing combinations [21] APPL NO; 469,458 of differential or selective media to separate mixtures of gram-positive and gram-negative bacteria, facilitate Related pp Data isolation of organisms from clinical specimens and [63] Continuation-impart of Ser. No. 329,084, Feb. 2, compare colony growth characteristics of pure cul- 1973, abandoned. tures. The tray has compartments to separate the media and the media is filled above the heights of the [52] U.S. CI. 195/139 barriers so that the swab or inoculating loop may [51] Int. (12 C12K 1/10 freely pass from one media to another, [58] Field of Search 195/1035, 139 I The aforementioned Abstract is neither intended to define the invention vof the application which, of [56] Referen'ces C'ted course, is measured by the claims, nor is it intended to UNITED STA TES PATENTS be limiting as to the scope of the invention in any way.
228 4 973 D l95 127 ll ummz 5 Claims, 2 Drawing Figures /D DRIVE 16 l I4 I: \4 23 I D P Lu 20d 20e k 9 MODIFIED PETRI DISH FOR AUTOMATIC INOCULATION This application is a continuation in part of my corresponding application Ser. No. 329,084 filed Feb. 2, 1973 now abandoned.
BACKGROUND OF THE INVENTION A need exists for more uniform methods of inoculation of plates and further to perform this repetitive task with a minimum of time on the part of trained technicians. To this end there is currently much effort being made to develop automatic inoculating machines. One of the problem areas is the development of a suitable tray which will permit the use of a simple mechanical movement to perform the inoculation.
Accordingly, it an object of this invention to provide an improved multi-media tray for use in an automatic inoculating machine.
SUMMARY OF THE INVENTION A multi-compartment dish is provided having ribs separating the compartments. Liquid media is filled slightly above the ribs and to gell in that condition whereby an inoculating tool may traverse from compartment to compartment without interference. Means are provided to engage the gelled media to prevent shifting in the event of shrinkage during gelling or upon drying in storage.
These and other features, objects and advantages of the invention will, in part, be pointed out with particularity and will, in part, become obvious from the following more detailed description of the invention, taken in conjunction with the accompanying drawing, which forms an integral part thereof.
In the various figures of the drawing like reference characters designate like parts.
BRIEF DESCRIPTION OF THE DRAWING FIG. 1 is a top plan view of the tray with its cover removed; and
FIG. 2 is a section taken along lines 2-2 of FIG. 1.
DESCRIPTION OF THE INVENTION The apparatus consists of a tray approximately l5 cm. by 7 cm. wide and about 4 cm. high. The outer walls 12 of the tray are preferably indented, as shown at 14 to receive a cover member 16. Partitions l8 and end barrier 19 extend about half the height, forming compartments 20a to 20d which contain media 22a to 22e. The media uses agar as a gelling material. Because of its cohesive characteristic it forms a meniscus providing a raised ridge 23.
The cover is removed and a specimen is swabbed across a media contained in compartment 20a using a loop or cotton swab. Transverse to compartment 201! there are compartments 20b, 20c 2011 and 20e containing any desired combination of selective or differential media such as gram-positive and gram-negative growth and inhibiting medias, blood agar, etc.
A sterile loop L is then actuated by a mechanical drive D and is then driven along path P to spread the culture along the media in a diluted fashion.
The specific media employed is not part of this invention and is within the province of the bacteriologist.
One advantage of automatic inoculation is that it avoids differences in technique between technicians providing greater uniformity. Further, the technician may perform other duties during inoculation.
A conventional inoculating loop or a cotton swab may be employed for uniform inoculation of agar media and colony isolation and combinations of media may be employed in one tray to isolate or differentiate between bacterial types. Further, this technique permits visualization of the overall qualitative, and to some extent, the quantitative relationships of the various bacterial types in the sample tested with uniformity.
The media has a tendency to shrink after filling and to stabilize the media pins 24 are provided which are integrally molded. The pins capture the solidified gelled media and prevent shifting.
Instead of pins the stabilizing means may be ribs, cleats or otherwise shaped protruding element.
Having thus disclosed the best embodiment of the invention presently contemplated, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit of the invention.
What I claim as new and desire to secure by Letters Patent is:
l. A media tray adapted for use with an automatic inoculating machine, said media tray comprising:
a. a plurality of adjacent compartments defined by a bottom wall and a series of ribs extending upwardly therefrom and forming an enclosure; and
b. bacteria growth supporting media contained within said compartments, at least a portion of the upper surface of said media extending above the uppermost portion of said ribs whereby automatically driven inoculating means may freely pass from the media in one compartment to the media in adjacent compartments; and
c. means extending from said bottom wall and partially extending into said media for immobilizing said media.
2. The tray of claim 1 including wall members surrounding the outer ones of said ribs, said wall members being higher than said ribs.
3. The tray of claim 2 including a removable cover adapted to fit over said wall members.
4. The tray of claim 1 including a plurality of first parallel compartments and a further compartment transverse to the said parallel compartments.
5. The tray of claim 1 wherein said means comprises pins integrally with said bottom wall.
* l l l

Claims (5)

1. A MEDIA TRAY ADAPTED FOR USE WITH AN AUTOMATIC INOCULATING MACHINE, MEDIA TRAY COMPRISING: A. A PLURALITY OF ADJACET COMPARTMENTS DEFINED BY A BOTTOM WALL AND SERIES OF RIBS EXTENDING UPWARDLY THEREFROM AND FORMING AN ENCLOSURE AND B. BACTERIA GROWTH SUPPORTING MEDIA CONTAINED WITHIN SAID COMPARTMENTS, AT LEAST A PORTION OF THE UPPER SURFACE OF SAID MEDIA EXTENDING ABOVE THE UPPERMOST PORTION OF SAID RIBS WHEREBY AUTOMATICALLY DRIVEN INOCULATING MEANS MAY FREELY PASS FROM THE MEDIA IN ONE COMPARTMENT TO THE MEDIA IN ADJACENT COMPARTMENTS, AND C. MEANS EXTENDING FROM SAID BOTTOM WALL AND PARTIALLY EXTENDING INTO SAID MEDIA FOR IMMOBILIZING SAID MEDIA.
2. The tray of claim 1 including wall members surrounding the outer ones of said ribs, said wall members being higher than said ribs.
3. The tray of claim 2 including a removable cover adapted to fit over said wall members.
4. The tray of claim 1 including a plurality of first parallel compartments and a further compartment transverse to the said parallel compartments.
5. The tray of claim 1 wherein said means comprises pins integrally with said bottom wall.
US469458A 1973-02-02 1974-05-13 Modified petri dish for automatic inoculation Expired - Lifetime US3902972A (en)

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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4012288A (en) * 1975-10-06 1977-03-15 Data Packaging Corporation Tissue culture cluster dish
US4204045A (en) * 1978-02-15 1980-05-20 Orion-Yhtyma Oy Device for examining microorganisms
US4283497A (en) * 1979-01-24 1981-08-11 Samson Helfgott Microbiological systems
US4345028A (en) * 1979-01-22 1982-08-17 Minnesota Mining And Manufacturing Company Bacteria growing device
FR2636073A1 (en) * 1988-09-02 1990-03-09 Escarguel Claude Improved dish for culture media which can be used in bacteriology
US4922430A (en) * 1987-10-30 1990-05-01 U.S. Philips Corporation Method and apparatus for controlling the movement of a guided object
WO1993024608A1 (en) * 1992-05-22 1993-12-09 Seaborn, George, Stephen Culture device for sampling and/or counting micro-organism
GB2283247A (en) * 1992-05-22 1995-05-03 Sue Kheng Chong Culture device for sampling and/or counting micro-organism
JPH07303470A (en) * 1994-05-11 1995-11-21 Nissui Pharm Co Ltd Fractionation medium and its production
EP0751215A2 (en) * 1995-06-29 1997-01-02 Becton, Dickinson and Company Culture vessel for use with coverslips
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms
US6156566A (en) * 1997-10-17 2000-12-05 Bryant; Debra L. In vitro fertilization procedure dish
WO2001055297A2 (en) * 2000-01-27 2001-08-02 Medigene Ag Three-dimensional matrix body, and device and method for measuring contractions of a cell tissue
KR100316321B1 (en) * 1999-04-23 2001-12-12 김상범 Kit for detecting microorganisms
WO2008083439A1 (en) * 2007-01-12 2008-07-17 Labtech Systems Limited Method and apparatus for inoculating and streaking a medium in a plate
US20100136671A1 (en) * 2007-05-30 2010-06-03 Nikon Corporation Incubation container
WO2020198326A1 (en) * 2019-03-26 2020-10-01 Xona Microfluidics, Inc. Apparatus and method for humidifying cell cultures

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3728228A (en) * 1971-10-12 1973-04-17 Md Labor Inc Device for indication of bacterial sensitivity to antibiotics

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3728228A (en) * 1971-10-12 1973-04-17 Md Labor Inc Device for indication of bacterial sensitivity to antibiotics

Cited By (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4012288A (en) * 1975-10-06 1977-03-15 Data Packaging Corporation Tissue culture cluster dish
US4204045A (en) * 1978-02-15 1980-05-20 Orion-Yhtyma Oy Device for examining microorganisms
US4345028A (en) * 1979-01-22 1982-08-17 Minnesota Mining And Manufacturing Company Bacteria growing device
US4283497A (en) * 1979-01-24 1981-08-11 Samson Helfgott Microbiological systems
US4922430A (en) * 1987-10-30 1990-05-01 U.S. Philips Corporation Method and apparatus for controlling the movement of a guided object
FR2636073A1 (en) * 1988-09-02 1990-03-09 Escarguel Claude Improved dish for culture media which can be used in bacteriology
US5695988A (en) * 1992-05-22 1997-12-09 Chong; Sue Kheng Culture device for sampling and/or counting micro-organism
GB2283247A (en) * 1992-05-22 1995-05-03 Sue Kheng Chong Culture device for sampling and/or counting micro-organism
GB2283247B (en) * 1992-05-22 1996-04-17 Sue Kheng Chong Culture device comprising a body of culture medium
WO1993024608A1 (en) * 1992-05-22 1993-12-09 Seaborn, George, Stephen Culture device for sampling and/or counting micro-organism
JPH07303470A (en) * 1994-05-11 1995-11-21 Nissui Pharm Co Ltd Fractionation medium and its production
EP0751215A2 (en) * 1995-06-29 1997-01-02 Becton, Dickinson and Company Culture vessel for use with coverslips
EP0751215A3 (en) * 1995-06-29 1997-03-05 Becton Dickinson Co Culture vessel for use with coverslips
JPH09117277A (en) * 1995-06-29 1997-05-06 Becton Dickinson & Co Culture container to be used together with cover glass
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms
US6156566A (en) * 1997-10-17 2000-12-05 Bryant; Debra L. In vitro fertilization procedure dish
KR100316321B1 (en) * 1999-04-23 2001-12-12 김상범 Kit for detecting microorganisms
WO2001055297A2 (en) * 2000-01-27 2001-08-02 Medigene Ag Three-dimensional matrix body, and device and method for measuring contractions of a cell tissue
WO2001055297A3 (en) * 2000-01-27 2002-02-07 Medigene Ag Three-dimensional matrix body, and device and method for measuring contractions of a cell tissue
US20030091979A1 (en) * 2000-01-27 2003-05-15 Thomas Eschenhagen Three-dimensional matrix body, and device and method for measuring contractions of a cell tissue
US7618452B2 (en) 2000-01-27 2009-11-17 Medigene Ag Artificially produced, three-dimensional muscle tissue
WO2008083439A1 (en) * 2007-01-12 2008-07-17 Labtech Systems Limited Method and apparatus for inoculating and streaking a medium in a plate
US20100173416A1 (en) * 2007-01-12 2010-07-08 Labtech Systems Limited Method and Apparatus for Inoculating and Streaking a Medium in a Plate
US8691558B2 (en) 2007-01-12 2014-04-08 Lbt Innovations Limited Method and apparatus for inoculating and streaking a medium in a plate
US9914953B2 (en) 2007-01-12 2018-03-13 Labtech Systems Ltd Method and apparatus for inoculating and streaking a medium in a plate
US20100136671A1 (en) * 2007-05-30 2010-06-03 Nikon Corporation Incubation container
JP2010527582A (en) * 2007-05-30 2010-08-19 株式会社ニコン Culture vessel
US8507261B2 (en) 2007-05-30 2013-08-13 Nikon Corporation Incubation container
WO2020198326A1 (en) * 2019-03-26 2020-10-01 Xona Microfluidics, Inc. Apparatus and method for humidifying cell cultures

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Owner name: FIRST CITY, A NATIONAL BANKING ASSOCIATION, TEXAS

Free format text: SECURITY INTEREST;ASSIGNOR:FLOW LABORATORIES, INC.;REEL/FRAME:005194/0930

Effective date: 19891108

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Owner name: FIRST CITY, TEXAS-HOUSTON, N.A., 1001 MAIN STREET,

Free format text: SECURITY INTEREST;ASSIGNOR:FLOW LABORATORIES, INC., A MD CORP.;REEL/FRAME:005554/0153

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