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• • C—CHEMISTRY; METALLURGY
  • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
  • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
  • C08G73/00—Macromolecular compounds obtained by reactions forming a linkage containing nitrogen with or without oxygen or carbon in the main chain of the macromolecule, not provided for in groups C08G12/00 - C08G71/00
  • C08G73/02—Polyamines
  • C08G73/026—Wholly aromatic polyamines
  • C08G73/0266—Polyanilines or derivatives thereof
• • H—ELECTRICITY
  • H01—ELECTRIC ELEMENTS
  • H01B—CABLES; CONDUCTORS; INSULATORS; SELECTION OF MATERIALS FOR THEIR CONDUCTIVE, INSULATING OR DIELECTRIC PROPERTIES
  • H01B1/00—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors
  • H01B1/06—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances
  • H01B1/12—Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of other non-metallic substances organic substances
  • H01B1/124—Intrinsically conductive polymers
  • H01B1/128—Intrinsically conductive polymers comprising six-membered aromatic rings in the main chain, e.g. polyanilines, polyphenylenes
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S977/00—Nanotechnology
  • Y10S977/70—Nanostructure
  • Y10S977/734—Fullerenes, i.e. graphene-based structures, such as nanohorns, nanococoons, nanoscrolls or fullerene-like structures, e.g. WS2 or MoS2 chalcogenide nanotubes, planar C3N4, etc.
  • Y10S977/742—Carbon nanotubes, CNTs
  • Y10S977/745—Carbon nanotubes, CNTs having a modified surface
  • Y10S977/746—Modified with biological, organic, or hydrocarbon material
  • Y10S977/747—Modified with an enzyme

Definitions

• polymers such as electrically conductive and optically active polymers (e.g. polythiophene, polypyrrole and polyaniline) for application to wider ranges of use.
• electrically conductive and optically active polymers e.g. polythiophene, polypyrrole and polyaniline
• examples of such uses include light-weight energy storage devices, electrolytic capacitors, anti-static and anti-corrosive coatings for smart windows, and biological sensors.
• the potential applications to which polymers can be put has been limited by some fundamental properties of monomers employed to form these polymers and by limitations of known polymerization techniques.
• polymeric materials is generally limited by an inability to manipulate the shape and orientation of polymer chains except by mechanical means, such as extrusion, or by polarization of relatively short polymers or oligomers in an electric field.
• synthetic methods of forming polymers generally do not provide means for manipulating their shape during polymerization.
• the present invention relates to a method for enzymatic template polymerization.
• the method includes combining a redox monomer with a template and an enzyme to form a reaction mixture.
• the redox monomer aligns along the template to form a complex and polymerizes.
• the method of the invention is an enzymatic polymerization in the presence of a polymeric or oligomeric electrolyte template which can lead to formation of unique structures.
• the template can cause an ordered polymerization of growing species, simultaneous charge transfer and complexation of the resulting polymer with the template.
• the template can have a unique conformation and properties that can be imparted to the growing polymer, giving the polymer unique functionalities.
• the resulting polymers can have a conformation that would not be produced in the absence of the template.
• the physical properties that can be controlled by the method of the invention are molecular weight, shape, electrical conductivity and optical activity.
• polymerization of a monomer or oligomer in the presence of the template can result in a polymer shell.
• the template can then be removed by decomposition, dissolution, or some other suitable method, to leave behind a polymer shell.
• Polymer shells can be predesigned to serve as useful architectures for drug design, drug delivery and molecular imprinting applications, among others.
• FIG. 1 shows the general mechanism of enzymatic polymerization of aniline in the absence of the polyelectrolyte, promoting ortho- and para-directed reactions.
• FIG. 2 shows a schematic of the template polymerization where a polyelectrolyte is dissolved in an aqueous solution and the monomer is then added under such conditions that complexation to the polyelectrolyte template occurs; upon addition of the peroxidase and initiator, the reaction takes place resulting in water-soluble polymer complex.
• FIG. 3 shows the chemical structure of oxidized (conducting) and reduced (insulating) forms of the polyaniline which is formed under the present conditions.
• FIG. 4 shows the visible absorption spectra of the polyaniline template complex (0.05M aniline to 0.1M SPS) formed at various pH's.
• FIG. 5 shows a plot of absorbance versus sulphonated polystyrene (SPS)/aniline ratio to find the optimum dopantto-monomer ratio.
• FIG. 6a shows the visible absorption and redox behavior of polyaniline/SPS prepared at pH 4.0 with increasing pH.
• FIG. 6b shows the visible absorbance and redox behavior of polyanilines/SPS (prepared at pH 4.0, then taken to pH 10) with decreasing pH.
• FIG. 7a shows the visible absorbance and redox behavior of a 50 bilayer film of poly(diallyl dimethyl ammonium chloride) (PDAC) alternating with SPS/polyaniline (prepared at pH 4.0, then taken to pH 10) with increasing pH.
• PDAC poly(diallyl dimethyl ammonium chloride)
• FIG. 7b shows the visible absorbance and redox behavior of a 50 bilayer film of SPS/polyaniline (prepared at pH 4.0) with decreasing pH.
• FIG. 8a shows the visible absorbance of polyphenol without SPS versus phenol monomer. Polyphenol precipitated out of solution as a result of polymerization.
• FIG. 8b shows the visible absorbance of polyphenol/SPS template versus phenol monomer, and wherein polyphenol did not precipitate out of solution.
• Enzyme-catalyzed polymerization of aniline and phenol typically involves reaction at the ortho and para positions of the aromatic ring as shown in FIG. 1. This mechanism often results in branched polymeric materials which are intractable and have negligible electrical and optical properties.
• This invention describes a novel template assisted enzymatic polymerization which results in a new class of polyanilines and polyphenols with electrical and optical stability, water solubility, processability and environmental compatibility.
• FIG. 2 gives a schematic of this template polymerization where first an aqueous solution containing a polyelectrolyte template and the monomer of interest (aniline and/or phenol) are prepared. Under the proper conditions, the monomer associates with the template and then upon addition of enzyme (peroxidase) and initiator (hydrogen peroxide) the polymerization proceeds resulting in a water-soluble polyaniline or polyphenol template complex.
• enzyme peroxidase
• initiator hydrogen peroxide
• the polyelectrolyte can serve at least three critical functions.
• the polyelectrolyte serves as a template upon which the monomers preferentially align themselves to form a complex, such as a chargetransfer complex, thereby promoting extended conjugation of the resulting polymer chains (limiting parasitic branching).
• a complex such as a chargetransfer complex
• the mechanism of polymerization is primarily para-directed and results in the electrically active form shown in FIG. 3. This preferential alignment provides improved electrical and optical properties of the final polymer complex.
• the polyelectrolyte can serve as a large molecular dopant species which is complexed and essentially locked to the polyaniline and/or polyphenol chains.
• the invention includes enzymatic template polymerization.
• a redox monomer is combined with a template and an enzyme to form a reaction mixture wherein the redox monomer aligns along the template to form a complex and polymerizes.
• a "template,” as that term is employed herein, is defined as a polymer or oligomer that can bind, such as by ionic binding, to the redox monomer being polymerized according to the method of the invention. It is believed that binding of such monomers can affect polymerization of adjacent monomers along the template polymer, thereby controlling the polymerization.
• the reaction solution is formed by adjusting the pH of a suitable solvent.
• the solvent is water.
• other components of the solvent can include, for example, dimethyl formamide, methanol, ethanol, dioxane, etc.
• the pH of the solvent is adjusted to a pH in a range of between about 4.0 and about 10.0.
• the pH is between about 4.0 and about 5.0 for aniline monomer and between about 6.0 and about 7.0 for phenol monomer.
• suitable buffers include Tris-HCl buffer, sodium phosphate, etc.
• the buffer is sodium phosphate buffer.
• a suitable enzyme is added to the reaction mixture.
• the concentration of enzyme in the reaction mixture is sufficient to significantly increase the polymerization rate of the monomer in the reaction solution.
• the concentration of enzyme in the reaction mixture is in a range of between about one unit/ml and about five units/ml where one unit will form 1.0 mg purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20° C.
• suitable enzymes include peroxidases, laccase, etc.
• Preferred enzymes are peroxidases.
• a particularly preferred enzyme is horseradish peroxidase.
• the monomer is added to the reaction mixture.
• suitable monomers include certain anilines and phenols, such as aniline, phenol, and derivatives of each, etc.
• the monomer can be a cation or an anion.
• the monomer can be, for example, a dye, such as an azo compound, or a ligand.
• an oligomer can be employed rather than a monomer. Mixtures of different monomers, oligomers, or of monomers and oligomers, can also be employed. In one embodiment, oligomers can form from the monomer prior to association or complexation with a template.
• the concentration of monomer in the reaction mixture generally is in a range of between about 10 mM and about 100 mM.
• a template polymer or oligomer then can be added to the reaction mixture.
• the template is added to the reaction mixture in a concentration that is sufficient to enable monomers to align along the template polymer during polymerization of the monomer and for the duration of the polymerization reaction.
• the solution is then suitable for enzyme-catalyzed polymerization.
• suitable template polymers include sulfonated polystyrene, sulfonated polystyrene polyion salts, polynucleotides, polypeptides, proteins, biological receptors, zeolites, caged compounds, azopolymers, and vinyl polymers, such as polyvinyl benzoic acid, polystyrene sulfonic acid and polyvinyl polyphosphonates, etc.
• Suitable template oligomers include deoxyribonucleotides, ribonucleotides, phenol red, azo compounds, etc.
• the template can be an anion or cation, such as a polyanion or a polycation.
• the template can be a polyelectrolyte, such as an optically active polyelectrolyte including, for example, azo polymers.
• the template can also be a dendrimer.
• the monomer or oligomer associates with the template to form, for example, a complex.
• the complex can be electrically or optically active.
• the polymerization reaction is a redox reaction and typically is initiated by adding a suitable oxidant, such as a hydrogen peroxide solution, etc.
• a suitable oxidant such as a hydrogen peroxide solution, etc.
• the hydrogen peroxide has a concentration in the solution in a range of between about one millimolar and about five millimolar.
• the concentration of hydrogen peroxide in the solution added to the reaction mixture is about 30%.
• the reaction mixture is stirred while slowly adding the hydrogen peroxide solution.
• the reaction mixture is maintained at a temperature in a range of between about 10° C. and about 25° C. during polymerization.
• the resulting polymer can be, for example, a linear polymer, such as an extended linear polymer intertwined with the polyelectrolyte template.
• the polymer can be dendritic, or branched. In any case, the polymer can have a conformation that would not be produced in the absence of the template.
• the polymer can be polyaniline complexed with a polyelectrolyte template, wherein the polyaniline is an extended linear polymer intertwined with the polyelectrolyte template.
• the polyaniline is a component of a water soluble electrically conducting complex.
• the temperature of the reaction mixture is maintained at a temperature of about 20° C. during polymerization.
• the method of the invention includes forming a layer of the polymer on a surface.
• the pH of the polymer solution is reduced to a suitable pH, such as a pH in a range of between about 2.0 and about 8.0, by adding a suitable acid, such as hydrochloric acid, etc.
• a suitable surface such as a glass slide treated with an alkali, such as CHEMSOLV® alkali, is immersed in a polymer solution for a sufficient period of time to cause the polymer to accumulate at the surface.
• a glass slide is immersed in a polymer solution for about ten minutes and then removed. The surface can then be washed with water at a pH of about 2.5 in order to remove unbound polymer from the surface.
• Distinct layers of polymers can be applied to a surface by this method.
• an initial layer can be formed by exposing a suitable surface to a polymer formed by the method of the invention that is a polyanion and then subsequently exposing the same surface, having the polyanion deposited upon it, into a solution of a polycation.
• a glass slide treated with CHEMSOLV® alkali is exposed to a one milligram/milliliter solution of poly(diallyl dimethyl ammonium chloride) at a pH of 2.5 as a polycation, and then exposed to a one milligram/milliliter solution of SPS/polyaniline formed by the method of the invention, as a polyanion.
• a bilayer of polymers is thereby formed. Additional layers of these or other polymers can subsequently be applied.
• polymerization of the template can be initiated simultaneously with, or subsequent to alignment and polymerization of the bound monomer or oligomer.
• the template can be removed from the resulting polymer, such as by decomposition or dissolution, to leave behind a polymer shell.
• the template-assisted enzymatic polymerization of aniline can be carried out in an aqueous solution using 0.1M sodium phosphate or tris-HCl buffer and a pH ranging from about 4.0 to about 10.0.
• Aniline monomer typically can be added in a range of between about 10 mM and about 100 mM, and an appropriate amount of a template, in this case sulphonated polystyrene (SPS) (molecular weight of 70,000), can be added in ratios ranging from about 1:10 to about 10:1 SPS/aniline.
• SPS sulphonated polystyrene
• the enzyme horseradish peroxidase then can be added to the reaction mixture in a range of approximately about one unit/ml to about five units/ml.
• an oxidizer such as hydrogen peroxide, slowly can be added in 10 ⁇ l increments over a reaction time of 3 hours, with constant stirring to a final concentration ranging from about 10 mM to about 100 m
• the template-assisted enzymatic polymerization of phenol can be carried out in an aqueous solution using 0.1M sodium phosphate or tris-HCl buffer and pH ranging from 4.0 to 10.0.
• Phenol monomer typically can be added in a range of between about 10 mM and about 100 mM and an appropriate amount of the template, sulphonated polystyrene (molecular weight of 70,000), can be added in ratios ranging from about 1:10 to about 10:1 SPS/phenol.
• the enzyme horseradish peroxidase then can be added to the reaction mixture in a range of approximately one unit/ml to five units/ml.
• an oxidizer such as hydrogen peroxide, slowly can be added in 10 ⁇ l increments over a reaction time of about 3 hours with constant stirring to a final concentration ranging from about 10 mM to about 100 mM.
• polymers formed by the method of the invention can be formed in an oxidized, electrically conducting form or in a reduced, insulating form of the polymer.
• Other physical properties of the polymers that can be affected by the method of the invention include the molecular weight and shape of the polymer.
• the polymers formed by the method of the invention can be modified after polymerization. For example, modification can be made at amine functional groups to form amides or imine groups.
• Dissolved polymers formed by the method of the invention can be precipitated from solution by adjusting the pH with a suitable acid or base.
• suitable acids or bases include hydrochloric acid, sodium hydroxide, etc.
• HRP horseradish peroxidase
• phosphate and Tris-HCl buffers were obtained from Sigma Chemicals Company, St. Louis, Mo.
• FIG. 4 shows the visible absorption spectra of the sulfonated polystyrene/polyaniline (SPS/PA) complex prepared under various pH conditions of 4, 6, 8, and 10. As shown in FIG. 4, SPS/PA, prepared at a pH of 4, exhibited a strong absorbance maximum at approximately 780 nm.
• FIG. 5 shows a plot of absorption maxima for various SPS/aniline ratios. As shown, a ratio of 1:2, SPS/aniline was the minimum ratio required to obtain the electrically conducting form of polyaniline, which had an absorption maximum at approximately 780 nm at a pH in a range of between about 4 and about 5.
• the reversible reduction/oxidation (redox) behavior of the SPS/PA complex was monitored by measuring visible absorption of the complex's under various pH conditions.
• the polymer complex was prepared at pH 4.0 to obtain the electrically active form of the polyaniline and then the pH of the solution was adjusted for the absorption maxima measurements.
• FIG. 6a the SPS/PA complex shifted in absorption maxima to shorter wavelengths as the pH of the solution was increased. This was indicative of reduction of the polyaniline backbone to a more insulating state.
• FIG. 6b shows the reverse behavior where the absorption maximum was found to shift back to longer wavelengths with decreasing pH conditions. This was indicative of oxidation of the polyaniline backbone back to a more electrically conductive state.
• FIGS. 7a and 7b show the visible absorption spectra of a film of fifty bilayers wherein PDAC layers alternate with SPS/PA layers, under various pH conditions.
• the multilayer film exhibited similar redox behavior as was observed previously with the solution absorption spectra. This confirmed that facile electrostatic deposition was feasible with the SPS/PA polymer complex and that the electrical activity was maintained after deposition.
• multilayer and bulk films were prepared on indium tin oxide (ITO) slides and four-point probe conductivity measurements were taken. The results gave polymer-complex conductivities in the range of 10 -3 to 10 2 S/cm.
• HRP horseradish peroxidase
• phosphate and Tris-HCl buffers were obtained from Sigma Chemicals Company, St. Louis, Mo.
• FIG. 8a shows the visible absorption of polyphenol without SPS, versus phenol monomer. As shown, there was a significant absorption maximum in the visible spectrum upon polymerization, indicating formation of polyphenol. However, with time the polymer began to precipitate out of solution.
• FIG. 8b shows the visible absorption of polyphenol with SPS, versus phenol monomer. As shown again, there was a significant absorption maximum of the polymerized system in the visible spectrum. In this case, there was no observed precipitation of the polymer complex out of solution.

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Citations (5)

• 1997
  • 1997-11-21 US US08/999,542 patent/US6018018A/en not_active Expired - Fee Related

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