WO1986006493A1 - Method and device for carrying out immunological assays - Google Patents
Method and device for carrying out immunological assays Download PDFInfo
- Publication number
- WO1986006493A1 WO1986006493A1 PCT/FI1986/000041 FI8600041W WO8606493A1 WO 1986006493 A1 WO1986006493 A1 WO 1986006493A1 FI 8600041 W FI8600041 W FI 8600041W WO 8606493 A1 WO8606493 A1 WO 8606493A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- particles
- rod
- magnetic
- equipment
- magnetic piece
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
Definitions
- the present invention is concerned with a fluorometric or phosphori etric im unoassay method in which small polymer particles are used as the solid phase,
- the method in accordance with the invention can be used, besides for immunoassays in general, also for blood group determinations.
- the separation of the solid phase from the reaction solution always includes washing of the solid phase, which at present, as a rule, requires manual operations. If small polymer particles are used, like in the method of the present invention, these operations include centrifuging or magnetic deposition.
- the object of the present' invention is to provide a simple manual method for the determination of antibodies or antigens , which said method is also suitable for use with such antibodies or antigens as are placed on the surface of cells or other particles of organic origin.
- tracer that emits radiation which said tracer may be in soluble form or preferably on polymer particles, is, together with particles that contain a magnetic mate ⁇ rial, immobilized on the antibody (or antigen) to be determined by means of an immunological bond.
- the magnetic particles and everything that has been immobilized on them by means of the immuno ⁇ logical bonds are pulled onto the face of a magnet to be submerged into the reaction solution.
- the magnet belonging to the apparatus in accordance with the inven- tion is preferably placed at the end of a rod, and the magnet is preferably provided with a protective cover, onto which the particles become positioned.
- the rod and the particles ahdering to it are pulled out of the reac ⁇ tion solution, and thereinafter the rod can be submerged into any washing and fixing solutions that may be needed.
- the rod with the particles adhering to it are, if desired, provided with a second protective cover and placed in a reading apparatus in accordance with the invention, wherein the radiation from the particle mass is measured in a way in itself known.
- the protective covers or one of them may be, in advance, provided with a substance affecting the intensity of the radiation signal, such as, e.g., a substrate of the enzyme used as the tracer, which substrate is made fluorescent by the enzyme.
- Figure 1 illustrates a reaction solution, in which, besides the sample to be studied, there are also magnetic and fluorescent particles.
- Figure 2 illustrates pulling of the particle mass onto the end of the magnetic rod.
- Figure 3 shows the reading apparatus.
- Figure 4 shows the rod with the magnet and with the protective covers attached to it.
- Figure 5 shows a protective cover to be placed on the magnet.
- a sample which contains antibody 1 and which has been diluted appropriately is placed in a reaction vessel 6 (Fig. 1).
- Magnetic particles 2 and fluorescent par- tides 3 are also administered into the vessel, both of which have been coated with an antigen 4 corresponding to the antibody 1.
- Ordinary incubation is carried out.
- the antibody 1 to be determined adheres both to the magnetic particles 2 and to the fluorescent particles 3, also causing adherence of these particles to each other. Without the intermediate of the antibody, the magnetic and the fluorescent particles cannot adhere to each other.
- a magnetic rod 5 (Fig. 2) is submerged into the reaction solution.
- the magnetic particles 2 and the fluorescent particles 3 pos ⁇ sibly adhering to them are collected by the effect of the magnet 11 onto the end of the rod 5.
- the rod 5 is lifted ' off the reaction solution and submerged in a washing solution. After washing, the rod 5 is placed into a measurement opening 8 provided in the reading apparatus 7 (Fig. 3) , in which said opening the fluorescence of the particle mass is measured by using an excitation light of suitable colour and by detecting the fluorescent radiation emitted from the particle mass.
- FIG. 4 shows the magnetic rod to be used in particular in the method.
- the rod comprises a tubular outer sleeve 9, composed of two parts connected to each other, and an inner rod 10 gliding in the sleeve. At the bottom end of the inner rod 10, there is a permanent magnet 11.
- the bottom end of the rod is conical, and onto the bottom end cup-shaped protective covers 12 and 13, placed one inside the other, have been pressed by means of a friction joint.
- the inner cover 12 is placed into its position before the rod is submerged into the reaction solution, and the outer cover 13 is pushed onto the inner cover after the washing stage.
- the protective covers are preferably disposable, and by their use it is possible to prevent contamination and wetting of the rod proper, on one hand, and of the measurement apparatus, on the other hand.
- the protective covers joined together can also be detached from the rod before measurement.
- the top end of the inner rod 10 extends to outside the outer sleeve 9, thus forming a press knob 14 by whose depression the covers 12 and 13 can be detached.
- a spiral spring 16 is fitted between the annular flange 15 and the bottom tip of the outer sleeve, which said spring pushes the inner rod to its upper position.
- the outer sleeve 9 is further provided with a limiter flange 17, and the inner rod with a shoulder 18, which prevent removing of the inner rod out of the sleeve.
- Figure 5 is a more detailed view of the pro ⁇ tective cover used on the rod.
- the cover is a cup made of a suitable material not interfering with the measure ⁇ ment, the bottom of the said cup being provided with feet 19.
- This sub ⁇ stance may be, e.g., a substrate of the enzyme used as the tracer, which substrate is made fluorescent by the enzyme.
- a fluorescent substance may also be placed into the cover, for which substance the radiation emitted from the sample acts as excitation radiation. In this way it is possible to transfer the signal to a longer, more readily detectable wavelength.
- a rod it is also possible to use an object of some other form which is provided with a magnet.
- the magnet may also be an electric magnet, in which case the rod must, of course, be provided with the necessary connection for the supply of electricity.
- Such an embodiment may be concerned if it is desirable to eliminate the magnetic field in between.
- the reading apparatus may be provided with automatic means which start the measurement immediately after the rod has been inserted into the measurement opening.
- the measurement equipment itself comprises a source of light, from which the excitation radiation is passed to the sample, a detector, into which the emission radiation is passed, as well as the necessary optics and equipment for the processing and display of the measurement result.
- reaction vessel can be shaped such that the rod can be pushed into it only up to a certain depth, whereby only the protective cover placed at the end of the rod becomes wet.
- the substance to be determined may, of course, be an antibody equally well as an antigen, and the radiation of the tracer may be, e.g., phosphorescent or radioactive radiation.
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI865002A FI865002A (en) | 1985-04-29 | 1986-12-08 | OVERFLOWING FOR IMPROVEMENT OF IMMUNOBETES. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI851702 | 1985-04-29 | ||
FI851702A FI851702A0 (en) | 1985-04-29 | 1985-04-29 | FOERFARANDE OCH ANORDNING FOER UTFOERING IMMUNOBESTAEMNINGAR. |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1986006493A1 true WO1986006493A1 (en) | 1986-11-06 |
Family
ID=8520741
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FI1986/000041 WO1986006493A1 (en) | 1985-04-29 | 1986-04-29 | Method and device for carrying out immunological assays |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0220255A1 (en) |
JP (1) | JP2727075B2 (en) |
FI (1) | FI851702A0 (en) |
WO (1) | WO1986006493A1 (en) |
Cited By (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987005536A1 (en) * | 1986-03-12 | 1987-09-24 | Carbomatrix Ab | Method and apparatus for collecting and dispersing ferromagnetic particles in a fluid medium |
WO1989011102A1 (en) * | 1988-05-04 | 1989-11-16 | Angenics, Inc. | Capillary flow device and double capture assay method |
EP0388940A1 (en) * | 1989-03-23 | 1990-09-26 | Hamamatsu Photonics K.K. | Method of modifying the surface of a magnetic particle |
GB2236852A (en) * | 1989-09-25 | 1991-04-17 | Scotgen Ltd | DNA probe/antibody based assays and intermediates useful in the synthesis of cleavable nucleic acids for use in such assays |
US5145784A (en) * | 1988-05-04 | 1992-09-08 | Cambridge Biotech Corporation | Double capture assay method employing a capillary flow device |
GB2270158A (en) * | 1992-08-03 | 1994-03-02 | Marconi Gec Ltd | Immunoassay using two detectable species |
GB2270976A (en) * | 1992-09-18 | 1994-03-30 | Marconi Gec Ltd | Immunoassay/separation process using an auxiliary species on a support |
GB2273157A (en) * | 1992-11-27 | 1994-06-08 | Marconi Gec Ltd | Immunological detection/separation using a plurality of immobilised binding agents |
WO1994018565A1 (en) * | 1993-02-01 | 1994-08-18 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
US5374531A (en) * | 1993-03-22 | 1994-12-20 | Zynaxis, Inc. | Immunoassay for determination of cells |
FR2708348A1 (en) * | 1993-07-28 | 1995-02-03 | Stago Diagnostica | Method for assaying an immunological substance using magnetic latex particles and non-magnetic particles. |
EP0679891A2 (en) * | 1989-11-17 | 1995-11-02 | Laboratoires Merck-Clévenot | Immunological diagnostic reagent |
WO1996012960A1 (en) * | 1994-10-20 | 1996-05-02 | Labsystems Oy | Separation of magnetic microparticles involving a pre-concentration step |
WO1996031777A1 (en) * | 1995-04-03 | 1996-10-10 | Macquarie Research Limited | Method for detecting microorganisms |
US5647994A (en) * | 1993-06-21 | 1997-07-15 | Labsystems Oy | Method and apparatus for separating magnetic particles from a solution |
US5723304A (en) * | 1992-08-03 | 1998-03-03 | Gec-Marconi Limited | Immunological detection using two detectable labels |
US5837144A (en) * | 1994-06-16 | 1998-11-17 | Boehringer Mannheim Gmbh | Method of magnetically separating liquid components |
DE19730497A1 (en) * | 1997-07-16 | 1999-02-11 | Heermann Klaus Hinrich Prof Dr | Magnetic pen for concentration and separation of particles |
AU706911B2 (en) * | 1994-09-19 | 1999-07-01 | Promega Corporation | Multisample magnetic separation device |
WO1999040444A1 (en) * | 1998-02-06 | 1999-08-12 | Bio-Magnetics Ltd. | A device and system for transfer of material |
US5942124A (en) * | 1994-10-20 | 1999-08-24 | Labsystems, Oy | Magnetic particle transfer device |
DE19823719A1 (en) * | 1998-05-27 | 1999-12-16 | Max Planck Gesellschaft | Method and device for processing small quantities of substances |
US6065605A (en) * | 1994-10-20 | 2000-05-23 | Labsystems Oy | Two-stage separation method |
WO2001000875A2 (en) * | 1999-06-25 | 2001-01-04 | Motorola, Inc. | Novel methods and products for arrayed microsphere analysis |
US6197597B1 (en) | 1993-02-01 | 2001-03-06 | Labsystems Oy | Solid phase immunoassay with carriers matching the shape of sample wells |
US6207463B1 (en) | 1994-10-20 | 2001-03-27 | Labsystems Oy | Separation device for microparticles involving a magnetic rod |
WO2008008257A2 (en) * | 2006-07-07 | 2008-01-17 | It Au0801213 | Oriented magnetic particle-fluorescence detectable moiety compositions and methods of making and using the same |
DE102009021201A1 (en) | 2009-05-13 | 2010-11-25 | Stratec Biomedical Systems Ag | Bar arrangement and method for extracting magnetizable particles from solutions |
CN102466732A (en) * | 2010-11-18 | 2012-05-23 | 南京神州英诺华医疗科技有限公司 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
US9417257B2 (en) | 2004-07-27 | 2016-08-16 | Nativis, Inc. | System and method for collecting, storing, processing, transmitting and presenting very low amplitude signals |
US10046172B2 (en) | 2013-03-15 | 2018-08-14 | Nativis, Inc. | Controller and flexible coils for administering therapy, such as for cancer therapy |
US11340244B2 (en) | 2019-03-15 | 2022-05-24 | Siemens Healthcare Diagnostics Inc. | Method and apparatus for magnetic bead manipulation |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5408259A (en) * | 1993-12-30 | 1995-04-18 | Northern Telecom Limited | Data modulation arrangement for selectively distributing data |
DE4421058A1 (en) * | 1994-06-16 | 1995-12-21 | Boehringer Mannheim Gmbh | Process for the magnetic separation of liquid components |
US6136549A (en) * | 1999-10-15 | 2000-10-24 | Feistel; Christopher C. | systems and methods for performing magnetic chromatography assays |
JP2010506172A (en) * | 2006-10-06 | 2010-02-25 | プロメガ・コーポレーション | Apparatus and method for separating magnetic particles from solution |
JP2011013042A (en) * | 2009-06-30 | 2011-01-20 | Beckman Coulter Inc | Automatic analysis device and measurement method |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3981776A (en) * | 1967-02-16 | 1976-09-21 | Rolf Saxholm | Magnetically responsive, biologically active substance and associated methods and apparatus |
US4115535A (en) * | 1977-06-22 | 1978-09-19 | General Electric Company | Diagnostic method employing a mixture of normally separable protein-coated particles |
US4272510A (en) * | 1976-04-26 | 1981-06-09 | Smith Kendall O | Magnetic attraction transfer process for use in solid phase radioimmunoassays and in other assay methods |
EP0136126A2 (en) * | 1983-09-09 | 1985-04-03 | Corning Glass Works | Magnetic separator for solid phase immunoassays |
EP0140787A2 (en) * | 1983-10-27 | 1985-05-08 | INSTITUT PASTEUR Fondation reconnue d'utilité publique | Magnetic means for the removal of magnetic gel particles from a test fluid |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4292920A (en) * | 1976-04-26 | 1981-10-06 | Smith Kendall O | Magnetic attraction transfer devices for use in solid phase radioimmunoassays and in other assay methods |
-
1985
- 1985-04-29 FI FI851702A patent/FI851702A0/en not_active Application Discontinuation
-
1986
- 1986-04-29 EP EP19860902834 patent/EP0220255A1/en not_active Withdrawn
- 1986-04-29 JP JP61502525A patent/JP2727075B2/en not_active Expired - Lifetime
- 1986-04-29 WO PCT/FI1986/000041 patent/WO1986006493A1/en not_active Application Discontinuation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3981776A (en) * | 1967-02-16 | 1976-09-21 | Rolf Saxholm | Magnetically responsive, biologically active substance and associated methods and apparatus |
US4272510A (en) * | 1976-04-26 | 1981-06-09 | Smith Kendall O | Magnetic attraction transfer process for use in solid phase radioimmunoassays and in other assay methods |
US4115535A (en) * | 1977-06-22 | 1978-09-19 | General Electric Company | Diagnostic method employing a mixture of normally separable protein-coated particles |
EP0136126A2 (en) * | 1983-09-09 | 1985-04-03 | Corning Glass Works | Magnetic separator for solid phase immunoassays |
EP0140787A2 (en) * | 1983-10-27 | 1985-05-08 | INSTITUT PASTEUR Fondation reconnue d'utilité publique | Magnetic means for the removal of magnetic gel particles from a test fluid |
Non-Patent Citations (1)
Title |
---|
Methods in Ensymology, Immunocheminiques, Part B, Vol 73 pages 471-82 Langone J.J. et al) * |
Cited By (52)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987005536A1 (en) * | 1986-03-12 | 1987-09-24 | Carbomatrix Ab | Method and apparatus for collecting and dispersing ferromagnetic particles in a fluid medium |
WO1989011102A1 (en) * | 1988-05-04 | 1989-11-16 | Angenics, Inc. | Capillary flow device and double capture assay method |
US5145784A (en) * | 1988-05-04 | 1992-09-08 | Cambridge Biotech Corporation | Double capture assay method employing a capillary flow device |
EP0388940A1 (en) * | 1989-03-23 | 1990-09-26 | Hamamatsu Photonics K.K. | Method of modifying the surface of a magnetic particle |
US5019416A (en) * | 1989-03-23 | 1991-05-28 | Hamamatsu Photonics K. K. | Method of modifying the surface of a particle comprising a magnetic particle |
GB2236852B (en) * | 1989-09-25 | 1994-04-06 | Scotgen Ltd | DNA probe based assays and intermediates useful in the synthesis of cleavable nucleic acids for use in such assays |
GB2236852A (en) * | 1989-09-25 | 1991-04-17 | Scotgen Ltd | DNA probe/antibody based assays and intermediates useful in the synthesis of cleavable nucleic acids for use in such assays |
EP0679891A3 (en) * | 1989-11-17 | 1995-11-29 | Merck Clevenot Lab | |
EP0679891A2 (en) * | 1989-11-17 | 1995-11-02 | Laboratoires Merck-Clévenot | Immunological diagnostic reagent |
US5723304A (en) * | 1992-08-03 | 1998-03-03 | Gec-Marconi Limited | Immunological detection using two detectable labels |
GB2270158B (en) * | 1992-08-03 | 1997-03-19 | Marconi Gec Ltd | Detection |
GB2270158A (en) * | 1992-08-03 | 1994-03-02 | Marconi Gec Ltd | Immunoassay using two detectable species |
GB2270976A (en) * | 1992-09-18 | 1994-03-30 | Marconi Gec Ltd | Immunoassay/separation process using an auxiliary species on a support |
GB2273157A (en) * | 1992-11-27 | 1994-06-08 | Marconi Gec Ltd | Immunological detection/separation using a plurality of immobilised binding agents |
EP1130397A2 (en) * | 1993-02-01 | 2001-09-05 | Thermo Labsystems Oy | Equipment for determination of an analyte from a sample |
US6040192A (en) * | 1993-02-01 | 2000-03-21 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
US6447729B1 (en) | 1993-02-01 | 2002-09-10 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
EP1130397A3 (en) * | 1993-02-01 | 2004-01-14 | Thermo Electron Oy | Equipment for determination of an analyte from a sample |
WO1994018565A1 (en) * | 1993-02-01 | 1994-08-18 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
US6197597B1 (en) | 1993-02-01 | 2001-03-06 | Labsystems Oy | Solid phase immunoassay with carriers matching the shape of sample wells |
US5374531A (en) * | 1993-03-22 | 1994-12-20 | Zynaxis, Inc. | Immunoassay for determination of cells |
US5647994A (en) * | 1993-06-21 | 1997-07-15 | Labsystems Oy | Method and apparatus for separating magnetic particles from a solution |
FR2708348A1 (en) * | 1993-07-28 | 1995-02-03 | Stago Diagnostica | Method for assaying an immunological substance using magnetic latex particles and non-magnetic particles. |
WO1995004279A1 (en) * | 1993-07-28 | 1995-02-09 | Societe Diagnostica-Stago | Method for assaying an immunological substance using magnetic latex particles and non-magnetic particles |
US5837144A (en) * | 1994-06-16 | 1998-11-17 | Boehringer Mannheim Gmbh | Method of magnetically separating liquid components |
AU706911B2 (en) * | 1994-09-19 | 1999-07-01 | Promega Corporation | Multisample magnetic separation device |
US6448092B1 (en) | 1994-10-20 | 2002-09-10 | Thermo Labsystems Oy | Separation device for microparticles involving a magnetic rod |
US6020211A (en) * | 1994-10-20 | 2000-02-01 | Labsystems Oy | Separation of magnetic microparticles involving a preconcentration step |
US6065605A (en) * | 1994-10-20 | 2000-05-23 | Labsystems Oy | Two-stage separation method |
WO1996012960A1 (en) * | 1994-10-20 | 1996-05-02 | Labsystems Oy | Separation of magnetic microparticles involving a pre-concentration step |
US5942124A (en) * | 1994-10-20 | 1999-08-24 | Labsystems, Oy | Magnetic particle transfer device |
US6207463B1 (en) | 1994-10-20 | 2001-03-27 | Labsystems Oy | Separation device for microparticles involving a magnetic rod |
US6225046B1 (en) | 1995-04-03 | 2001-05-01 | Macquarie Research Ltd. | Method for detecting microorganisms |
WO1996031777A1 (en) * | 1995-04-03 | 1996-10-10 | Macquarie Research Limited | Method for detecting microorganisms |
DE19730497C2 (en) * | 1997-07-16 | 2000-02-10 | Heermann Klaus Hinrich | Method for washing, separating and concentrating biomolecules using a magnetic pen |
DE19730497A1 (en) * | 1997-07-16 | 1999-02-11 | Heermann Klaus Hinrich Prof Dr | Magnetic pen for concentration and separation of particles |
US6409925B1 (en) * | 1998-02-06 | 2002-06-25 | Bio-Magnetics Ltd. | Device and system for transfer of material |
WO1999040444A1 (en) * | 1998-02-06 | 1999-08-12 | Bio-Magnetics Ltd. | A device and system for transfer of material |
DE19823719B4 (en) * | 1998-05-27 | 2011-12-15 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for concentrating substances |
DE19823719A1 (en) * | 1998-05-27 | 1999-12-16 | Max Planck Gesellschaft | Method and device for processing small quantities of substances |
US7105357B1 (en) | 1998-05-27 | 2006-09-12 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E. V. | Method and device for processing extremely small substance quantities |
WO2001000875A2 (en) * | 1999-06-25 | 2001-01-04 | Motorola, Inc. | Novel methods and products for arrayed microsphere analysis |
WO2001000875A3 (en) * | 1999-06-25 | 2001-09-13 | Motorola Inc | Novel methods and products for arrayed microsphere analysis |
US9417257B2 (en) | 2004-07-27 | 2016-08-16 | Nativis, Inc. | System and method for collecting, storing, processing, transmitting and presenting very low amplitude signals |
WO2008008257A3 (en) * | 2006-07-07 | 2008-02-28 | Nativis Inc | Oriented magnetic particle-fluorescence detectable moiety compositions and methods of making and using the same |
WO2008008257A2 (en) * | 2006-07-07 | 2008-01-17 | It Au0801213 | Oriented magnetic particle-fluorescence detectable moiety compositions and methods of making and using the same |
DE102009021201A1 (en) | 2009-05-13 | 2010-11-25 | Stratec Biomedical Systems Ag | Bar arrangement and method for extracting magnetizable particles from solutions |
US8454825B2 (en) | 2009-05-13 | 2013-06-04 | Stratec Biomedical Ag | Rod assembly and a method for the extraction of magnetizable particles from solutions |
CN102466732A (en) * | 2010-11-18 | 2012-05-23 | 南京神州英诺华医疗科技有限公司 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
US10046172B2 (en) | 2013-03-15 | 2018-08-14 | Nativis, Inc. | Controller and flexible coils for administering therapy, such as for cancer therapy |
US11103721B2 (en) | 2013-03-15 | 2021-08-31 | Natives, Inc. | Controller and flexible coils for administering therapy, such as for cancer therapy |
US11340244B2 (en) | 2019-03-15 | 2022-05-24 | Siemens Healthcare Diagnostics Inc. | Method and apparatus for magnetic bead manipulation |
Also Published As
Publication number | Publication date |
---|---|
EP0220255A1 (en) | 1987-05-06 |
JP2727075B2 (en) | 1998-03-11 |
FI851702A0 (en) | 1985-04-29 |
JPS62502708A (en) | 1987-10-15 |
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