WO1991006369A2 - Temperature-equalization chamber for bringing the contents of a microtitration plate to a uniform temperature - Google Patents
Temperature-equalization chamber for bringing the contents of a microtitration plate to a uniform temperature Download PDFInfo
- Publication number
- WO1991006369A2 WO1991006369A2 PCT/AT1990/000107 AT9000107W WO9106369A2 WO 1991006369 A2 WO1991006369 A2 WO 1991006369A2 AT 9000107 W AT9000107 W AT 9000107W WO 9106369 A2 WO9106369 A2 WO 9106369A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- temperature
- heating
- temperature control
- plate
- chamber according
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
Definitions
- Temperature control chamber especially for temperature control of the contents of a microtitration plate
- the invention relates to a temperature control chamber, in particular for temperature control of the content of a microtitration plate with a housing with a base plate designed as a temperature control plate, which has a controllable heat exchanger, which is preferably formed by an electrical, plate-shaped temperature control element and one Cover cap.
- Such temperature chambers are used in particular in the photometric measurement of blood samples.
- temperature devices are required which are suitable for microcuvettes and conventional microtitration plates in the existing measuring devices, e.g. Photometers can be used.
- Temperature control chambers are known from the reading device, according to the more recent state of the art these are advantageously arranged directly next to or in the same, so that there is no change in temperature of the liquid contained in the microtitration plate when the latter is fed to the reading device.
- the temperature control units require a quick warm-up and a corresponding temperature constancy (e.g. 37 degrees C. +/- 0.3 degrees C.).
- a corresponding temperature constancy e.g. 37 degrees C. +/- 0.3 degrees C.
- the object of the invention is to provide a temperature control device of the type mentioned at the outset which ensures better and more uniform heating or cooling of the contents of microtitration plates. It should also be possible to keep the temperature constant for as long as possible. This allows measurements to be carried out over a longer period of time, e.g. B. in the form of a series of measurements with several individual measurements at a distance of z. B. five minutes each.
- An embodiment of the invention provides that the bottom plate has two heat exchangers, one heat exchanger surrounding the other and being assigned to the edge of the microtiter plate.
- tempering means warming up because, for example, blood samples have to be warmed from room temperature (approx. 20 ° C.) or refrigerator temperature (approx. 4 ° C.) to body temperature (approx. 37 ° C.). In countries with high air temperature, however, the temperature control chamber can also be used for cooling.
- the perforating chamber as a heating chamber, since this is the more frequently occurring case. It is advantageously provided that the heat exchangers are formed by heating circuits, for example by electrical resistance heating elements.
- the construction according to the invention makes it possible, for example, to set the inner heating circuit to a higher temperature than the outer heating circuit during heating, so that a compensation is created for the cavities of the microtitration plate and all cavities are heated essentially the same regardless of their position become.
- the outer heating circuit is set to a temperature which is increased with respect to the inner heating circuit.
- a further exemplary embodiment of the invention provides that a heating circuit is assigned to the base plate and a heating circuit to the cover, the cover being designed like a flap and covering the microtitration plate like a bell.
- the heat exchangers are formed by electrical temperature control elements which are embedded in foils or formed by heat-conducting plates.
- a separation joint is provided in the film or plate between two heat exchangers.
- the heat exchangers can be, for example, heating circuits of electrical resistance heaters.
- the invention is not intended to be restricted to electrical temperature control elements, it could also be heated with pipes which carry a liquid, the construction with electrical temperature control elements nevertheless has constructive advantages, since such heaters are relatively are to be produced professionally.
- the parting line between the foils results in better thermal delimitation of the heating and cooling circuits.
- one or more temperature sensors are provided which are in contact with this when the microtiter plate is inserted; the starting temperature of the plate can be determined via these sensors or any temperature change during the reading process can be determined and via a corresponding one Control of the temperature control unit can be compensated.
- the method for heating microtitration plates provides that the starting temperature and the target temperature of the contents of a microtitration plate are entered in a computer and, after taking the filling quantity into account, the amount of energy required to achieve the target temperature is calculated.
- the method according to the invention makes it possible to significantly shorten the temperature setting, ensuring that the contents of the microtitration plate are not heated above the specified target temperature.
- the heating-up time is calculated on the basis of the filling quantity.
- the heating temperature can be chosen empirically.
- FIG. 1 shows a diagram of a reading device for microtitration plates with a temperature chamber according to the invention
- FIG. 2 shows a schematic top view of the device according to FIG. 1
- FIG. 3 shows a longitudinal section through the temperature chamber
- FIG. 4 shows a cross section through the temperature chamber
- FIG. 5 shows the support of the outer edge of the cover in section
- FIG. 6 shows a plan view of a temperature control element according to the invention, which FIG. 7 shows a temperature diagram and
- FIG. 8 shows a circuit for performing the method according to the invention.
- the actual reading device 1 and the temperature control chamber 2 are arranged directly next to one another and accommodated in a common housing 3.
- the temperature control chamber 2, whose dimensions are adapted to a microtitration plate 4, is covered by a cover 5, which is designed as a flap-like cover.
- the microtitration plate 4 rests on a slide 6 which is guided on rods 7 and moves back and forth between the temperature control chamber 2 and the reading device 1.
- the microtitration plate 4 is therefore inserted into the temperature control chamber 2 and the cover 5 is closed.
- the temperature chamber 2 is heated or cooled until the contents of the cavities in the microtitration plate 4 have reached the desired target temperature (generally 37 ° C.), whereupon the microtitration plate 4 is not exposed to room temperature, which in turn causes a change in temperature
- the desired target temperature generally 37 ° C.
- a temperature control film 8 is arranged on the bottom of the temperature chamber 2.
- the tempering film 8 is usually not necessarily a heating film.
- the tempering foil 8 is covered by an aluminum foil 9. Mainly, the microtitration plate 4 is heated from below by the temperature control film 8.
- a temperature control film 10 is also provided on the underside of the cover 5, which in turn is covered with an aluminum film 9.
- the tempering film 10 is assigned a separate heating or cooling circuit.
- the lower temperature control film 8 is divided and has an inner heat exchanger 11 and an outer heat exchanger 12.
- heating or cooling could also be installed in which a fluid is the heat transfer medium.
- Ceramic or metallic plates with an applied semiconductor layer can also be used.
- cooling devices can be used.
- the heating chamber 2 is now provided with three heating circuits.
- the heating circuits of the heat exchangers 11 and 12 are controlled in such a way that, during the heating phase, the heat exchanger 11 emits more heat than the heat exchanger 12. This ensures uniform heating of all cavities in the microtitration plate.
- the heating circuit of the heat exchanger 12 After reaching the target temperature, the heating circuit of the heat exchanger 12 emits more heat than the heating circuit of the heat exchanger 11 over the duration of the stable phase, in which it is only important to keep the temperature constant, in order to compensate for the increased heat dissipation of the microtitration plate 4 at the edges .
- sensors 13, 14 are also provided, which bear against the microtitration plate 4.
- the temperatures which are measured by the sensors 13, 14 and passed on to the computer 18 correspond at least approximately to the temperature which really prevails in the interior of an adjacent cavity of the microtitration plate 4.
- the cover 5 projects with an edge web 15 into a groove 16 in the side wall 17 of the heat chamber 2. This forms an obstacle that extends the path of the air flow.
- the temperature values can be entered manually into the computer 16 or can also be detected by sensors and passed on directly.
- the filling quantity of the liquid contained in the cavitates is entered into the computer 18, from which the computer calculates the amount of energy required for heating or cooling the contents of the microtitration plate 4 to the desired target temperature or the warming-up or cooling-down period.
- the warm-up time is given on the abscissa in minutes, while the ordinate shows the temperature.
- the curve FT shows the course of the temperature of the liquid contained in the cavities of the microtitration plate 4, the curve WT shows the course of the temperature of a heat exchanger 9, 11, 12.
- Target temperature of z. B. 37 ° C can be reached quickly, without running the risk that the contents of the micro plate 4 ever, apart from a predetermined tolerance range, heats up above this value.
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI913192A FI913192A0 (en) | 1989-11-02 | 1991-07-01 | TEMPERERINGSKAMMARE, SAERSKILT FOER TEMPERERING AV INNEHAOLLET I EN MIKROTITRERINGSPLATTA. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT252489A AT394323B (en) | 1989-11-02 | 1989-11-02 | TEMPERATURE CHAMBER, ESPECIALLY FOR TEMPERING THE CONTENT OF A MICROTITRATION PLATE, AND METHOD FOR CONTROLLING A HEATING CIRCUIT |
ATA2524/89 | 1989-11-02 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1991006369A2 true WO1991006369A2 (en) | 1991-05-16 |
WO1991006369A3 WO1991006369A3 (en) | 1991-08-08 |
Family
ID=3535818
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/AT1990/000107 WO1991006369A2 (en) | 1989-11-02 | 1990-11-02 | Temperature-equalization chamber for bringing the contents of a microtitration plate to a uniform temperature |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0451242A1 (en) |
JP (1) | JPH04502580A (en) |
AT (1) | AT394323B (en) |
WO (1) | WO1991006369A2 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0488769A2 (en) * | 1990-11-29 | 1992-06-03 | The Perkin-Elmer Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
DE4217868A1 (en) * | 1992-05-29 | 1993-12-02 | Univ Schiller Jena | Temp.-controlled multiple test tube for optical study of liquids - has upper and lower aluminium plates having openings into which test tubes are placed at least one plate has surface foil heating element |
DE19646115A1 (en) * | 1996-11-08 | 1998-05-14 | Eppendorf Geraetebau Netheler | Temperature control block with temperature control devices |
US6372486B1 (en) | 1998-11-30 | 2002-04-16 | Hybaid Limited | Thermo cycler |
US6767512B1 (en) | 1996-11-08 | 2004-07-27 | Eppendorf Ag | Temperature-regulating block with temperature-regulating devices |
DE19655141B4 (en) * | 1996-11-08 | 2005-04-07 | Eppendorf Ag | Heating block for laboratory thermostats - has different heating fields for rapid setting of required temperature profile |
DE19655282B4 (en) * | 1996-11-08 | 2005-04-07 | Eppendorf Ag | Heating block for laboratory thermostats - has different heating fields for rapid setting of required temperature profile |
DE102009015869A1 (en) | 2009-04-01 | 2010-10-21 | Schneckenburger, Herbert, Prof. Dr. | Tempering device for tempering two-dimensionally arranged proteins in micro titer plate, has resistor elements attached at adapted admixture in regions, where measurements of sample parameters are accomplished during adjustment of sensor |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4492466B2 (en) * | 2005-07-11 | 2010-06-30 | 株式会社島津製作所 | Sample thermostat |
DE102010019232B4 (en) * | 2010-05-03 | 2013-06-27 | Eppendorf Ag | Avoid condensation hood |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0092140A1 (en) * | 1982-04-19 | 1983-10-26 | BEHRINGWERKE Aktiengesellschaft | Microtitration plate |
US4584275A (en) * | 1981-07-31 | 1986-04-22 | Fuji Photo Film Co., Ltd. | Incubator |
DE3441179A1 (en) * | 1984-11-10 | 1986-05-22 | Dynatech Deutschland GmbH, 7306 Denkendorf | Temperature-control device for microcell arrangements, in particular microtitration plates |
US4679615A (en) * | 1984-03-02 | 1987-07-14 | Advanced Products Ltd. | Method and apparatus for heating and/or cooling objects simultaneously at different preselected temperatures |
WO1989012502A1 (en) * | 1988-06-23 | 1989-12-28 | Lep Scientific Limited | Biochemical reaction machine |
DE3941168A1 (en) * | 1988-12-13 | 1990-06-21 | Interconnection B V | Heated micro-titration plate - has cuvettes, plate-shaped member, temp. probes and sensors, ceramic plate and heating elements |
EP0388159A2 (en) * | 1989-03-15 | 1990-09-19 | Seiko Instruments Inc. | Apparatus for sealing liquid within cavities |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3513385A1 (en) * | 1985-04-15 | 1986-10-16 | Devappa Dr.Ing. Zinsser & Ing. K.Prestl, 8420 Kelheim | Temperature-regulating device |
-
1989
- 1989-11-02 AT AT252489A patent/AT394323B/en not_active IP Right Cessation
-
1990
- 1990-11-02 WO PCT/AT1990/000107 patent/WO1991006369A2/en not_active Application Discontinuation
- 1990-11-02 EP EP19900915705 patent/EP0451242A1/en not_active Withdrawn
- 1990-11-02 JP JP51465190A patent/JPH04502580A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4584275A (en) * | 1981-07-31 | 1986-04-22 | Fuji Photo Film Co., Ltd. | Incubator |
EP0092140A1 (en) * | 1982-04-19 | 1983-10-26 | BEHRINGWERKE Aktiengesellschaft | Microtitration plate |
US4679615A (en) * | 1984-03-02 | 1987-07-14 | Advanced Products Ltd. | Method and apparatus for heating and/or cooling objects simultaneously at different preselected temperatures |
DE3441179A1 (en) * | 1984-11-10 | 1986-05-22 | Dynatech Deutschland GmbH, 7306 Denkendorf | Temperature-control device for microcell arrangements, in particular microtitration plates |
WO1989012502A1 (en) * | 1988-06-23 | 1989-12-28 | Lep Scientific Limited | Biochemical reaction machine |
DE3941168A1 (en) * | 1988-12-13 | 1990-06-21 | Interconnection B V | Heated micro-titration plate - has cuvettes, plate-shaped member, temp. probes and sensors, ceramic plate and heating elements |
EP0388159A2 (en) * | 1989-03-15 | 1990-09-19 | Seiko Instruments Inc. | Apparatus for sealing liquid within cavities |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6015534A (en) * | 1990-11-29 | 2000-01-18 | The Perkin-Elmer Corporation | PCR sample tube |
US5710381A (en) * | 1990-11-29 | 1998-01-20 | The Perkin-Elmer Corporation | Two piece holder for PCR sample tubes |
US5602756A (en) * | 1990-11-29 | 1997-02-11 | The Perkin-Elmer Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
US5475610A (en) * | 1990-11-29 | 1995-12-12 | The Perkin-Elmer Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
EP0488769A2 (en) * | 1990-11-29 | 1992-06-03 | The Perkin-Elmer Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
EP0812621A1 (en) * | 1990-11-29 | 1997-12-17 | The Perkin-Elmer Corporation | Automated performance of the polymerase chain reaction |
EP0488769A3 (en) * | 1990-11-29 | 1993-05-26 | Perkin-Elmer Cetus Instruments | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
DE4217868A1 (en) * | 1992-05-29 | 1993-12-02 | Univ Schiller Jena | Temp.-controlled multiple test tube for optical study of liquids - has upper and lower aluminium plates having openings into which test tubes are placed at least one plate has surface foil heating element |
DE19646115A1 (en) * | 1996-11-08 | 1998-05-14 | Eppendorf Geraetebau Netheler | Temperature control block with temperature control devices |
DE19646115C2 (en) * | 1996-11-08 | 2000-05-25 | Eppendorf Geraetebau Netheler | Use of temperature control devices for temperature control of a temperature control block |
US6767512B1 (en) | 1996-11-08 | 2004-07-27 | Eppendorf Ag | Temperature-regulating block with temperature-regulating devices |
DE19655141B4 (en) * | 1996-11-08 | 2005-04-07 | Eppendorf Ag | Heating block for laboratory thermostats - has different heating fields for rapid setting of required temperature profile |
DE19655282B4 (en) * | 1996-11-08 | 2005-04-07 | Eppendorf Ag | Heating block for laboratory thermostats - has different heating fields for rapid setting of required temperature profile |
DE19655141C5 (en) * | 1996-11-08 | 2013-12-05 | Eppendorf Ag | Gradient tempering block for laboratory thermostats |
US6372486B1 (en) | 1998-11-30 | 2002-04-16 | Hybaid Limited | Thermo cycler |
DE102009015869A1 (en) | 2009-04-01 | 2010-10-21 | Schneckenburger, Herbert, Prof. Dr. | Tempering device for tempering two-dimensionally arranged proteins in micro titer plate, has resistor elements attached at adapted admixture in regions, where measurements of sample parameters are accomplished during adjustment of sensor |
Also Published As
Publication number | Publication date |
---|---|
AT394323B (en) | 1992-03-10 |
JPH04502580A (en) | 1992-05-14 |
WO1991006369A3 (en) | 1991-08-08 |
ATA252489A (en) | 1991-09-15 |
EP0451242A1 (en) | 1991-10-16 |
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