WO1992002536A1

WO1992002536A1 - Systematic polypeptide evolution by reverse translation

Info

Publication number: WO1992002536A1
Application number: PCT/US1991/005463
Authority: WO
Inventors: Larry Gold; Craig Tuerk
Original assignee: The Regents Of The University Of Colorado
Priority date: 1990-08-02
Filing date: 1991-08-01
Publication date: 1992-02-20
Also published as: AU8498091A

Abstract

The method of systematic polypeptide evolution by reverse translation (SPERT) includes a candidate mixture of polypeptides having randomized amino acid sequence. Each member of the mixture is linked to an individualized mRNA which encodes the amino acid sequence of that polypepide.

Description

SYSTEMATIC POLYPEPTIDE EVOLUTION

BY REVERSE TRANSLATION This work was supported by grants from the United States Government funded through the National Institutes of Health. The U.S.

Government has certain rights in this invention. FIELD OF THE INVENTION

We describe herein novel high-affinity polypeptide ligands that specifically bind a desired target molecule. A method is presented for selecting a polypeptide ligand that

specifically binds any desired target molecule. The method is termed SPERT, an acronym for

Systematic Polypeptide Evolution by Reverse

Translation. The method of the invention (SPERT) is useful to isolate a polypeptide ligand for a desired target molecule. The polypeptide products of the invention are useful for any purpose to which a binding reaction may be put, for example in assay methods, diagnostic procedures, cell sorting, as inhibitors of target molecule

function, as probes, as sequestering agents and the like. In addition, polypeptide products of the invention can have catalytic activity. Target molecules include natural and synthetic polymers, including proteins, polysaccharides,

glycoproteins, hormones, receptors and cell surfaces, nucleic acids, and small molecules such as drugs, metabolites, cofactors, transition state analogs and toxins. BACKGROUND OF THE INVENTION

As translation of mRNA proceeds, stable complexes are formed. These complexes are made of ribosomes bound to mRNA with tRNA and nascent polypeptide encoded by the messenger RNA. Termed "ribosome complexes" herein, such complexes can be isolated by various known processes (Connolly and Gilmore (1986) J. Cell Biol. 103: 2253; Perara et al, (1986) Science 232:348). Antigen-encoding mRNAs have been purified by taking advantage of the immunoreactivity of nascent polypeptides associated with ribosome complexes (Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, NY) (1989) ibid, sections 8.9-8.10). Such immunoreactive ribosome complexes can be immunoprecipitated from solution or separated by protein A column

chromatography from non-reactive ribosome

complexes (Schutz et al. (1977) Nuc. Acids Res. 4, 71; Shapiro and Young (1981) J. Biol. Chem. 256, 1495). Cyclical selection and amplification of RNAs with partitionable properties is now also possible. Historically, mRNA selection is closely tied to immunopurification of ribosome complexes, however, the partitioning of ribosome complexes according to the present invention is not

restricted to immunoreactivity of the nascent polypeptides. SUMMARY OF THE INVENTION

In its broadest aspect, the method of systematic polypeptide evolution by reverse translation (SPERT) includes a candidate mixture of polypeptides having a randomized amino acid sequence. Each member of the mixture is linked to an individualized mRNA which encodes the amino acid sequence of that polypeptide. The candidate polypeptides are partitioned according to their property of binding to a given desired target molecule. The partitioning is carried out in such a way, herein described, that each mRNA encoding a polypeptide is partitioned exactly together with that polypeptide. In this way each polypeptide is partitioned together with the means for further amplifying it by an In vitro process. Ultimately, both the desired optimal polypeptide ligand of the desired target and the mRNA encoding the

polypeptide are simultaneously selected, allowing further synthesis of the selected polypeptide as desired, and further amplification of the coding sequence. It is therefore not necessary to analyze the amino acid sequence of the selected polypeptide (using protein chemistry) in order to produce it in desired quantities.

Viewed another way, the invention is the selective evolution of a nucleic acid that encodes a polypeptide ligand of a desired target. The present method is therefore a selection based upon coding properties available in a candidate nucleic acid mixture. In a previously filed application, U.S. Serial No. 07/536,428, filed June 11, 1990, incorporated herein by reference, the inventors herein have taught a method for selective

evolution of nucleic acids based upon binding properties of the nucleic acids themselves. The insight that cyclical selection and amplification can be a powerful tool for developing novel compounds when coupled with a partitioning system is herein adapted to evolving specific coding nucleic acids, based on the partitioning

properties of polypeptide ligands binding to target molecules.

More specifically, the invention

includes a method for making a polypeptide ligand of a desired target molecule which includes the following steps: First, synthesizing a mixture of translatable mRNA's, having certain sequence segments in common such as a ribosome binding site and a translation initiation codon and having a segment encoding a polypeptide at least part of which coding region is a randomized sequence.

Second, employing the mRNA mixture in an in vitro translation system. Synthesis of nascent

polypeptides ensues, each encoded by its own mRNA. At any time during translation, stable ribosome complexes can be isolated. It is preferred to isolate complexes in which translation has been stopped, or "stalled" by any of several known circumstances. Each isolated ribosome complex includes at least one ribosome, one nascent peptide and the coding mRNA which is now said to be translated mRNA. Although its chemical

structure is unaltered, translated mRNA is bound to the ribosome complex in a different manner than it was bound prior to translation, as is known in the art. Third, the ribosome complexes are partitioned with respect to the binding of each nascent polypeptide to a desired target molecule. Some polypeptides bind weakly, some tightly, some not at all, with the target. The partitioning, however conducted, generally separates the mixture of ribosome complexes into ribosome complex-target pairs and unbound complexes. The set of ribosome complex-target pairs is thereby enriched for those polypeptides (and, necessarily their coding mRNA's) that can bind to the target. Fourth, the encoding mRNA's are separated from the complexes and amplified by conventional means for amplifying nucleic acids, such as reverse transcription and polymerase chain reaction (PCR). This

amplification sets the stage for a subsequent round of transcription, polypeptide synthesis and partitioning to further enrich for target-binding polypeptide ligands. These cycles can be

reiterated as many times as desired, until a desired binding affinity is achieved, or no further improvement in binding affinity is

observed. The coding mRNA for any polypeptide selected in the foregoing manner can be cloned and sequenced, if desired. An individual polypeptide ligand can then be prepared in vivo from cloned coding mRNA, or by chemical or enzymatic methods in vitro.

The present invention provides a class of products which are polypeptides, each having a unique sequence, each of which has the property of binding specifically to a desired target compound or molecule. Each compound of the invention is a specific ligand of a given target molecule. The invention is based on the unique insight that cyclical selection and amplification of nucleic acids can be applied to coding sequences by partitioning such coding sequences according to the binding affinities of the encoded

polypeptides. In vitro evolutionary selection can therefore be applied for the first time to polypeptides. Polypeptides have sufficient capacity for forming a variety of two- and three- dimensional structures and sufficient chemical versatility available within their monomers to act as ligands (form specific binding pairs) with virtually any chemical compound, whether monomeric or polymeric. Molecules of any size can serve as targets. Most commonly, and preferably, for therapeutic applications, binding takes place in aqueous solution at conditions of salt,

temperature and pH near acceptable physiological limits. For other uses different binding

conditions can be employed.

The invention also provides a method which is generally applicable to make a

polypeptide ligand for any desired target. The method involves selection from a mixture of candidates and step-wise iterations of structural improvement, using the same general selection theme, to achieve virtually any desired criterion of binding affinity and selectivity.

While not bound by a theory of operation, SPERT is based on the inventors' insight that within a polypeptide mixture

containing a large number of possible sequences and structures there is a wide range of binding affinities for a given target. A polypeptide mixture comprising, for example a 10 amino acid randomi •zed segment can have 2010 candidate

possibilities. Those which have the higher affinity constants for the target are most likely to bind. After partitioning ribosome complexes, dissociation of mRNA and reverse

transcription/amplification/ transcription, a second polypeptide mixture is generated by

translation, enriched for the higher binding affinity candidates. Additional rounds of SPERT progressively favor the best ligands until the resulting polypeptide mixture is predominantly composed of only one or a few sequences. These can then be individually synthesized and tested for binding affinity as pure ligands. One cycle of SPERT effectively achieves reverse translation, at least quantitatively.

Cycles of selection and amplification are repeated until a desired goal is achieved. In the most general case, selection/amplification is continued until no significant improvement in binding strength is achieved on repetition of the cycle. The iterative selection/amplification method is sensitive enough to allow isolation of a single sequence variant in a mixture containing at least 65,000 sequence variants. The method could, in practice, be used to sample about 10¹⁸ different polypeptide species. There is no upper limit, in principle, to the number of different polypeptides which could be sampled, only a practical limit dictated by the sizes of reaction vessels and other containers necessary to perform the method. The polypeptides of the test mixture include a randomized sequence portion as well as conserved sequences as desired for combining with other functional domains or to provide sufficient polypeptide length to insure that the randomized sequence is accessible to the target in the ribosome complex. Amino acid sequence variants can be produced in a number of ways including chemical or enzymic synthesis of randomized nucleic acid coding sequences. The variable sequence portion may contain fully or partially random sequence; it may also contain subportions of conserved sequence incorporated with randomized sequence. Sequence variation in coding nucleic acids can be introduced or increased by

mutagenesis before or during the

selection/amplification iterations.

In the case of a polymeric target, such as a protein, the ligand affinity can be increased by applying SPERT to a mixture of candidates comprising a first selected polypeptide sequence combined with a second randomized sequence. The sequence of the first selected ligand associated with binding or subportions thereof can be

introduced into the randomized portion of the amino acid sequence of a second test mixture. The SPERT procedure is repeated with this second test mixture to isolate a second polypeptide ligand, having two sequences (one being the first

polypeptide ligand) selected for binding to the target, which has increased binding strength or increased specificity of binding compared to the first polypeptide ligand isolated. The sequence of the second polypeptide ligand associated with binding to the target can then be introduced near the variable portion of the amino acid sequence after which cycles of SPERT results in a third polypeptide ligand. The third polypeptide ligand also contains the first and second ligand

previously selected. These procedures can be repeated until a polypeptide ligand of a desired binding strength or a desired specificity of binding to the target molecule is achieved. The process of iterative selection and combination of polypeptide sequence elements that bind to a selected target molecule is herein designated "walking," a term which implies the optimized binding to other accessible areas of a

macromolecular target surface or cleft, starting from a first binding domain. Increasing the area of binding contact between ligand and target can increase the affinity constant of the binding reaction. These walking procedures are

particularly useful for isolating novel

polypeptides which are highly specific for binding to a particular target molecule.

A variant of the walking procedure employs a ligand termed "anchor" which is known to bind to the target molecule at a first binding domain (See Figure 8). This anchor molecule can in principle be any molecule that binds to the target molecule and which can be covalently linked directly or indirectly to a small bridge molecule for which a peptide binding sequence is known. When the target molecule is an enzyme, for

example, the anchor molecule can be an inhibitor or substrate of that enzyme. The anchor can also be an antibody or antibody fragment specific for the target. The anchor molecule is covalently linked to the bridge molecule, chosen to bind an oligopeptide of known sequence. A test mixture of candidate polypeptides is then prepared which includes a randomized portion and includes also the known sequence that binds the bridging

molecule. The bridging molecule binds the

polypeptides to the target molecule in the

vicinity of the anchor binding site. SPERT is then applied to select polypeptides which bind a surface of the target molecule adjacent to the anchor binding site. Polypeptide ligands which bind to the target are isolated. Walking

procedures as described above can then be applied to obtain polypeptide ligands with increased binding strength or increased specificity of binding to the target. Walking procedures could employ selections for binding to the anchor binding site itself or to another part of the target itself. This method is particularly useful to isolate polypeptide ligands which bind at a particular site within the target molecule. The anchor acts to ensure the isolation of polypeptide sequences which bind to the target molecule at or near the binding site of the anchor.

Screens, selections or assays to assess the effect of binding of a polypeptide ligand on the function of the target molecule can be readily combined with the SPERT methods. Specifically, screens for inhibition or activation of enzyme activity can be combined with the SPERT methods.

In more specific embodiments, the SPERT method provides a rapid means for isolating and identifying polypeptide ligands which bind to nucleic acids and proteins, including enzymes, receptors, antibodies, and glycoproteins.

In another aspect, the present invention provides a method for detecting the presence or absence of, and/or measuring the amount of a target molecule in a sample, which method employs a polypeptide ligand which can be isolated by the methods described herein. Detection of the target molecule is mediated by its binding to a polypeptide ligand specific for that target molecule. The polypeptide ligand can be labeled, for example radiolabeled or enzyme linked, to allow qualitative or quantitative detection, analogous to ELISA and RIA methods. The detection method is particularly useful for target molecules which are proteins. The method is more

particularly useful for detecting proteins which are known to be only weakly antigenic, or for which conventional monoclonal antibodies of a desired affinity are difficult to produce. Thus, polypeptide ligands of the present invention can be employed in diagnostics in a manner similar to conventional antibody-based diagnostics. One advantage of polypeptide ligands over conventional antibodies in such detection methods and

diagnostics is that polypeptides are capable of being readily synthesized in vitro or after cloning, since the method of the invention

concomitantly selects the means for amplification, e.g., coding nucleic acids, along with the ligand itself. Alternatively, the polypeptide can be chemically synthesized since its amino acid sequence can be ascertained readily from the nucleotide sequence of its coding mRNA. A SPERT-generated polypeptide ligand need not be as large as an antibody molecule. Another advantage is that the entire SPERT process is carried out in vitro and does not require immunizing test

animals. Furthermore, the binding affinity of polypeptide ligands can be tailored to the user's needs. Compared to antibodies, SPERT-generated ligands have much greater versatility.

Conventional antibodies are immunoglobulins, which, although capable of a large repertoire of binding affinities, are nevertheless variations of a narrow amino acid sequence and structural theme. SPERT-generated polypeptide ligands, in contrast, are unlimited as to structural type, and therefore have virtually unlimited potential for binding.

Polypeptide ligands of small molecule targets are useful as diagnostic assay reagents and have therapeutic uses as sequestering agents, drug delivery vehicles and modifiers of hormone action. Catalytic polypeptides are selectable products of this invention. For example, by selecting for binding to transition state analogs of an enzyme catalyzed reaction, catalytic

polypeptides can be selected. Catalytic

immunoglobulins have been developed by raising antibodies to transition state analogs (Schultz, P.C. (1989) Angew. Chem. Int. 2d Engl. 28:1283-1295; Schultz, P.G. (1989) Ace. Chem. Res. 22:287-294; Pollack, S.J. et al. (1989) Meth. Enzymol. 178:551-568).

In yet another aspect, the present invention provides a method for modifying the function of a target molecule using polypeptide ligands which can be isolated by SPERT.

Polypeptide ligands which bind to a target

molecule are screened to select those which specifically modify function of the target

molecule, for example to select inhibitors or activators of the function of the target molecule. An amount of the selected polypeptide ligand which is effective for modifying the function of the target is combined with the target molecule to achieve the desired functional modification. This method is particularly applicable to target molecules which are proteins. A particularly useful application of this method is to inhibit protein function, for example to inhibit receptor binding or to inhibit enzyme catalysis. In this case, an amount of the selected polypeptide molecule which is effective for target protein inhibition is combined with the target protein to achieve the desired inhibition.

The term "reverse translation" is used throughout as shorthand for the concept of information flow from polypeptide sequence to nucleic acid sequence. The phrase and shorthand make reference to the original and revised

"central dogma" pronounced by Francis Crick many years ago. Crick understood and articulated the idea that either RNA or DNA could serve as a template for the synthesis of complementary nucleic acid sequences, and that chemically either RNA or DNA could serve as a template for the synthesis of both RNA and DNA. Crick noted that proteins, comprised of strings of amino acids, were templated by nucleic acid but could not serve themselves as a template for the synthesis of nucleic acids.

Most importantly, no simple chemistry is known that allows "reverse translation"; that was the basis nearly 25 years ago of Crick's adaptor hypothesis for using information in RNA to yield specified protein sequences during translation.

SPERT has at its center a form of reverse translation that does not conflict with Crick's postulates. While no process, no simple chemistry, is known that provides synthesis of a nucleic acid containing a sequence specified by a polypeptide (whose sequence is unknown to the scientist at the time of reverse translation), SPERT provides a reliable mechanism for amplifying and using mRNAs that encode polypeptides of desired function but of unknown sequence.

Techniques for binding one or a few polypeptides to a selected target are known in the art, although binding of a small number of polypeptides from a randomized pool of polypeptides is of no value by itself. It is the concomitant selection in the ribosome complex of the mRNAs that encode those very polypeptides that provides a form of reverse translation because:

1) the selected coding sequences can be amplified to yield large quantities of both DNA and RNA;

2) the newly made mRNA can be used for synthesizing polypeptides, now a smaller set than the original randomized mixture of polypeptides from which non-binding, or poorly-binding

polypeptides have been removed, and;

3) the polypeptides held in ribosome complexes can be used for a subsequent round of SPERT.

Finally, "reverse translation" during SPERT does not yield a nucleic acid from only polypeptide sequence, but "reverse translation" does provide (through amplification techniques) net synthesis of the templates from which the desired polypeptide was synthesized. In principle a single molecule of polypeptide of the desired activity, along with a single template RNA in the translation complex, will lead to a nanomole or even a micromole of nucleic acid corresponding to that polypeptide sequence. This net synthesis of nucleic acids based on the partitioning and activity of the desired polypeptide is an

effective quantitative reverse translation that provides the materials for subsequent rounds of SPERT.

Also, the coding sequence can be used to deduce the amino acid sequence of a selected polypeptide. The polypeptide can then be

synthesized by chemical methods, if desired.

BRIEF DESCRIPTION OF THE FIGURES

Figure 1 is a diagrammatic

representation of steps in the process of the invention. The top panel depicts a double-stranded DNA template having a T7 promoter ("T7 PRO") and a segment of randomized sequence, represented as "nnn. . . . .. " , preceded by a start codon, ATG. The initiation site of transcription and direction of transcription are shown as a vertical line labeled "+1" and an arrow,

respectively. In vitro transcription creates mRNAs (2nd panel) which contain, from left to right, a ribosome binding site, a randomized sequence region, a 3' fixed sequence region, and a 3* primer annealing site. In vitro translation of this mixture gives rise to ribosome complexes with randomized nascent polypeptides (3rd panel). The ribosome complexes are subjected to selection for affinity cf the nascent polypeptide and a desired target molecule (bottom panel). The encoding mRNAs of the partitioned complexes are purified and subjected to amplification, e.g., by reverse transcription, PCR and transcription, to generate mRNAs for a second cycle of the process.

Figure 2 is a diagram showing expanded views of a ribosome complex. The top panel is a ribosome complex as in the third panel of Figure 1. A cut-away view of the ribosome (2nd panel) shows 30-40 amino acids of the nascent polypeptide buried in the complex and unavailable for

interaction with the solvent. The ribosome is depicted with two shades of gray to indicate inner and outer regions. The nascent polypeptide is depicted as a thick white line extending

vertically from a central tunnel (black) near the center of the ribosome. That portion inside the ribosome is depicted as 30-40 amino acids in length. The carboxy-terminal end of the nascent polypeptide is shown connected to a peptidyl-tRNA (curly black line). The region bordered by a dotted line is expanded in the bottom panel showing that the nascent polypeptide is covalently linked to a transfer RNA molecule which is

hydrogen-bonded to the mRNA at a codon in the P-site.

Figure 3 is a diagram that represents partitioning polypeptide ligands by direct

immunoprecipitation. The top panel is a ribosome complex as in Figure 1. The center panel depicts several ribosome complexes where the nascent polypeptide is represented as a short, thick white line with hatching to indicate the segment of randomized sequence. Molecules of a first

antibody (immunoglobulin) are represented as inverted Y-shaped structures drawn with heavy, straight black lines. Interaction (binding) of a nascent polypeptide with the epitope recognition site of an immunoglobulin is shown for two ribosome complexes. Nascent polypeptides are selected that have affinity for immunoglobulin molecules. The bottom panel shows addition of a second antibody (white inverted Y's) generally reactive to the first immunoglobulin resulting in an immunoprecipitate containing the selected ribosome complexes, shown as a cluster in the left half of the panel.

Figure 4 is a diagram showing partitioning of polypeptide ligands by indirect immunoprecipitation. The top panel shows a target protein which has an immunoreactive domain

("handle") and a target domain ("pan"). Three types of ribosome complexes are depicted in the second panel. Those with no affinity for the target protein are shown in white. Those with affinity for the "pan" are shown in light gray labeled with a "P" and shown with a bound target protein attached by the "pan" to the nascent peptide. Those with affinity for the "handle" are dark gray, labeled with an "H" and shown with a bound target protein attached by the "handle" to the nascent peptide. In the third panel, a first antibody (black lines) directed against the

"handle" either displaces ligand associations of the "H" complexes or those complexes are

unreactive. The first antisera form a sandwich with the "P" complexes made up of a ribosome complex associated with the target protein, through its "pan", and bound to the first

immunoglobulin through the "handle". These "P" complexes are immunoprecipitated by second antisera directed against the primary antisera, as shown in the bottom panel.

Figure 5 is a diagram showing selection of polypeptide ligands by membrane partitioning. The top panel shows a ribosome complex as in

Figure 1. The middle panel shows ribosome

complexes and membrane vesicles with membrane proteins. The membrane vesicles are depicted as a hatched band interrupted by hatched ovals that depict membrane proteins embedded in the membrane. In the middle panel, ribosome complexes are shown binding with membrane protein so that the nascent polypeptides having binding affinity for a

membrane protein are partitioned. The bottom panel depicts three ribosome complexes bound to a membrane vesicle, forming a large complex which is separable from unbound ribosome complexes.

Figure 6 is a diagram showing partitioning of polypeptide ligands by affinity column chromatography. Ribosome complexes (top panel) are passed through a column containing insoluble support materials to which have been bonded target molecules. The middle panel is an expanded view of the column showing support materials (hatched circular segments) with

attached target molecules (black bars) to which some ribosome complexes are bound. The bottom panel shows, enlarged, a single ribosome complex in which the nascent polypeptide (light shading) is bound to a target molecule which is attached to a column support bead (hatched). Ribosome

complexes with high affinity to the target

molecules are retained on the column and

subsequently eluted to continue with SPERT. Figure 7 is a diagram showing anchoring of a binding epitope and secondary ligand

evolution. A molecule ("inhibitor") of known affinity for a target site on a protein is

covalently linked to a "guide epitope". The guide epitope is any molecule for which there exists a peptide ligand, including a portion of a

monoclonal antibody which contains an epitope recognition domain (Fab fragment). The mRNA encodes a reactive peptide sequence that binds the guide epitope, incorporated into the nascent polypeptide. The bottom panel depicts a ribosome complex having a nascent polypeptide that includes the reactive, guide binding, segment (shaded) and a randomized segment (unshaded) . The ribosome complex is shown bound to the protein of interest by a binding interaction between the guide epitope and the reactive segment and by a secondary binding interaction between the randomized segment and a neighborinq site on the target protein of interest. The randomized portion of the nascent polypeptide is free to evolve interactions with secondary sites on the target protein.

Figure 8 is a diagram which shows the DNA to be transcribed and the relationships of the oligonucleotides of Tables 1 and 2 in the DNA, prior to inserting the randomized sequence. The depicted structure constitutes a cassette for carrying out the transcription, translation, reverse transcription and PCR processes used in SPERT.

DETAILED DESCRIPTION OF THE INVENTION

The following terms are used herein according to the definitions.

Polypeptide is used herein to denote any string of amino acid monomers capable of being synthesized by an in vitro translation system. The term also embraces post-translational

modifications introduced by chemical or enzymecatalyzed reactions, as are known in the art.

Such post-translational modifications can be introduced prior to partitioning, if desired.

Unless specified herein, all amino acids will be in the L-stereoisomeric form. Amino acid analogs can be employed instead of the 20 naturally-occurring amino acids. Any amino acid analog that is recognized by an aminoacyl-tRNA synthetase can be employed. Several such analogs are known, including fluorophenylalanine, norleucine,

azetidine-2-carboxylic acid, S-aminoethyl

cysteine, 4-methyl tryptophan and the like.

Ligand means a polypeptide that binds another molecule (target). In a population of candidate polypeptides, a ligand is one which binds with greater affinity than that of the bulk population. In a candidate mixture there can exist more than one ligand for a given target.

The ligands can differ from one another in their binding affinities for the target molecule.

Candidate mixture is a mixture of nucleic acids and of polypeptides of differing sequence, from which to select a desired coding sequence or a desired ligand. The candidate mixture of nucleic acids serving as source of a candidate mixture of polypeptides can be in vitro transcription products of naturally-occurring nucleic acids or fragments thereof, chemically synthesized nucleic acids, enzymatically

synthesized nucleic acids or nucleic acids made by a combination of the foregoing techniques.

Target molecule means any compound of interest for which a ligand is desired. A target molecule can be a protein, fusion protein,

peptide, enzyme, nucleic acid, nucleic acid binding protein, carbohydrate, polysaccharide, glycoprotein, hormone, receptor, receptor ligand, cell membrane component, antigen, antibody, virus, virus component, substrate, metabolite, transition state analog, cofactor, inhibitor, drug,

controlled substance, dye, nutrient, growth factor, toxin, lipid, glycolipid, etc., without limitation.

Partitioning means any process whereby ribosome complexes bound to target molecules, termed complex-target pairs herein, can be

separated from ribosome complexes not bound to target molecules. Partitioning can be

accomplished by various methods known in the art. The only requirement is a means to separate complex-target pairs from unbound ribosome

complexes. Columns which selectively bind

complex-target pairs but not ribosome complexes, (or specifically retain ligand to an immobilized target) can be used for partitioning. A membrane or membrane fragment having the target on its surface can bind ligand-bearing ribosome complexes forming the basis of a partitioning based on particle size. The choice of partitioning method will depend on properties of the target and of the complex-target pairs and can be made according to principles and properties known to those of ordinary skill in the art.

Amplifying means any process or combination of process steps that increases the amount or number of copies of a molecule or class of molecules. Amplifying coding mRNA molecules in the disclosed examples is carried out by a

sequence of three reactions: making cDNA copies of selected mRNAs, using polymerase chain reaction to increase the copy number of each cDNA, and

transcribing the cDNA copies to obtain an

abundance of mRNA molecules having the same sequences as the selected mRNAs. Any reaction or combination of reactions known in the art can be used as appropriate, including direct DNA

replication, direct mRNA amplification and the like, as will be recognized by those skilled in the art. The amplification method should result in the proportions of the amplified mixture being essentially representative of the proportions of different sequences in the mixture prior to amplification.

Specific binding is a term which is defined on a case-by-case basis. In the context of a given interaction between a given ligand and a given target, a binding interaction of ligand and target of higher affinity than that measured between the target and the candidate ligand mixture is observed. In order to compare binding affinities, the conditions of both binding

reactions must be the same, and should be

comparable to the conditions of the intended use. For the most accurate comparisons, measurements will be made that reflect the interaction between ligand as a whole and target as a whole. The polypeptide ligands of the invention can be selected to be as specific as required, either by establishing selection conditions that demand the requisite specificity during SPERT, or by

tailoring and modifying the ligands through

"walking" and other modifications using iterations of SPERT.

Randomized is a term used to describe a segment of a nucleic acid or polypeptide having, in principle any possible sequence over a given length. Randomized nucleic acid sequences will be of various lengths, as desired, ranging from about twelve to more than 300 nucleotides. The chemical or enzymatic reactions by which random sequence segments are made may not yield mathematically random sequences due to unknown biases or

nucleotide preferences that may exist. Redundancy of the genetic code, and biases in the tRNA content of an in vitro translation system can introduce additional bias in the translated amino acid sequences. Introducing a deliberate bias into a randomized coding region can reduce the bias of the resulting translated amino acid sequence. The term "randomized" is used instead of "random" to reflect the possibility of such deviations from non-ideality. In the techniques presently known, for example sequential chemical synthesis, large deviations are not known to occur.

A bias may be deliberately introduced into a randomized sequence, for example, by altering the molar ratios of precursor nucleoside (or deoxynucleoside) triphosphates of the

synthesis reaction. A deliberate bias may be desired, for example, to improve the randomness of amino acid sequence of translated polypeptides or to lower the frequency of appearance of certain amino acids.

For example, a randomized sequence biased for codons of the form ARN (where A is Adenine, R is Adenine or Guanine and N is any nucleotide) the most commonly encoded amino acids are basic (Arg, Asn, Lys) or polar (Ser).

Randomized sequences biased for codons of the form GRN are biased for acidic amino acids, Asp (GAU, GAC) and Glu (GAA, GAG) , and Glycine (GGN).

Randomized sequences in which U is never the 1st base in the triplet codon will lack termination signals and will not encode amino acids Phe, Tyr, Cys and Trp. By such strategies, randomized coding sequences can be biased for the type of structure likely to bind a given target. For example, polypeptide sequences biased for acidic amino acids can bind cationic target molecules more easily than completely random polypeptides.

Translatable mRNA is RNA which possesses all requisite sequences for translation in a conventional in vitro translation system. These include, inproper orientation and sequence

proximal to the 5' end of the RNA, a ribosome binding site and an initiation codon. In

prokaryotes, as is known in the art, other codons, such as UUG and GUG can serve as initiation codons and encode methionine if properly spaced within a ribosome binding site.

Ribosome binding site means a nucleotide sequence in the mRNA which functions as a binding site for a ribosome in an in vitro translation system. The sequences which function as ribosome binding sites differ depending on whether the ribosomes are of procaryotic or eucaryotic origin, as is known in the art. In procaryotic systems, the ribosome binding site is a short purine-rich region with a sequence such as GAGG or AGGA, usually located about 5 - 12 bases 5' to the initiation codon. The translation initiation codon is therefore usually located within 5 - 12 bases from the ribosome binding site in the 3' direction on the mRNA. These sequences are sometimes termed a Shine-Dalgarno sequence. The structures of ribosome binding sites and their proper placement to ensure correct initiation of protein synthesis are well known in the art.

Initiation codon is a characteristic trinucleotide sequence AUG which encodes

methionine and which encodes a first amino acid of an encoded polypeptide and also sets the codon reading frame for the nucleotide sequence in the 3' direction from the initiation codon.

Ribosome complex is a macromolecular complex including at least one ribosome, attached mRNA molecule and, for each ribosome, a nascent polypeptide attached via tRNA to the ribosome.

The nascent polypeptide has an amino acid sequence encoded by the attached mRNA. Ribosome complexes are formed, as is known in the art, during protein synthesis. Ribosome complexes are stable if they become stalled for any reason, for example, by depletion of release factor, lack of termination codon in the message, lack of a charged tRNA, etc., as known in the art. The mRNA together with attached ribosome(s) and nascent peptide (s) remain stably bound and can be isolated together, using methods known in the art.

In vitro translation can be carried out using known systems. These well-known translation systems are the Ε. coli system, the wheat germ system, and the rabbit reticulocyte system. The latter is available commercially. The conditions for carrying out in vitro translations are well-known in the art, and various modifications, adaptations and optimizations are available to those skilled in the art.

The combination of translatable mRNA encoding a polypeptide and in vitro translation system constitute amplifying means for amplifying the quantity of polypeptide encoded by the mRNA. The mRNA can itself be amplified using reverse transcription, PCR with appropriate primers and an RNA polymerase. The amplified mRNA can serve for in vitro synthesis of desired quantities of the encoded polypeptide. As noted, supra. this process constitutes reverse translation.

The terms "ribosome" and "nascent peptide" have conventional meanings known in the art. The term "translated mRNA" simply refers to mRNA present in a ribosome complex, either wholly or partially translated.

Ribosome complex-target pairs are ribosome complexes of which the nascent

polypeptide component is bound to a target

molecule. The target molecule can be free in solution or bound to a solid support matrix.

Homology is used to compare the related uses of sequences. Percent amino acid sequence homology is measured by comparing sequences of equal length position by position. The percent of those positions occupied by the same amino acid in two polypeptides is the percent sequence homology. Thus, given peptide ABCDE as a naturally-occurring comparison peptide, peptides ABCDX or ABXDE are

80% homologous but peptides ABXYZ, AXYZE and XYZDE are 40% homologous and peptides EDCBA, BDAEC, MNOPQ are non-homologous.

The SPERT method involves the combination of a selection of polypeptide ligands which bind to a target molecule, for example a protein, with amplification of those selected polypeptides via the attached mRNAs. Iterative cycling of the selection/ amplification steps allows selection of one or a small number of polypeptides which bind most strongly to the target from a pool which contains a very large number of nucleic acids and hence encoded

polypeptides.

Cycling of the selection/amplification procedure is continued until a selected goal is achieved. For example, cycling can be continued until a desired level of binding of the

polypeptides in the test mixture is achieved or until a minimum number of polypeptide components of the mixture is obtained (in the ultimate case until a single species remains in the test

mixture). In many cases, it will be desired to continue cycling until no further improvement of binding is achieved. It may be the case that certain test mixtures of polypeptides show limited improvement in binding over background levels during cycling of the selection/ amplification. In such cases, the sequence and length variation in the test mixture should be increased until improvements in binding are achieved. Anchoring protocols and/or walking techniques can be

employed as well.

Specifically, the method requires the initial preparation of a test mixture of candidate polypeptides. A translatable mRNA mixture is prepared, each member of the mixture including in its nucleotide sequence a ribosome binding site, an initiation codon and a randomized coding region. Preferably the individual mRNA's contain a randomized region flanked by sequences conserved in all nucleic acids in the mixture. The

conserved regions are provided to facilitate amplification of selected nucleic acids. Since there are many such sequences known in the art, the choice of sequence is one which those of ordinary skill in the art can make, having in mind the desired method of amplification. The

randomized coding region can have a fully or partially randomized sequence according to the desired translation product. Depending on the desired polypeptide structure, the coding portion of the nucleic acid can contain subportions that are randomized, along with subportions which are held constant in all nucleic acid species in the mixture. For example, sequence regions known to code for amino acid sequences that bind, or have been selected for binding, to the target can be integrated with randomized coding regions to achieve improved binding or improved specificity of binding. Sequence variability in the

polypeptide test mixture can also be introduced or augmented by generating mutations in the coding mRNA's during the selection/amplification process. In principle, the mRNA's employed in the test mixture can be any length as long as they can be amplified. The method of the present invention is most practically employed for selection from a large number of sequence variants. Thus, it is contemplated that the present method will

preferably be employed to assess binding of polypeptide sequences ranging in length from about four amino acids to any attainable size.

The randomized portion of the coding nucleic acids in the test mixture can be derived in a number of ways. For example, full or partial sequence randomization can be readily achieved by direct chemical synthesis of the nucleic acid (or portions thereof) or by synthesis of a template from which the nucleic acid (or portions thereof) can be prepared by use of appropriate enzymes.

Chemical synthesis provides the advantages of being precisely controllable as to length and allowing individual randomization at each triplet position. A commercial DNA synthesizer can be used, either with an equivalent mixture of the four activated nucleotide substrates or with a biased mixture. Alternatively, the synthesizer can be set up to provide a limited nucleotide selection at a given position, e.g., only A at the first triplet position. End addition, catalyzed by terminal transferase in the presence of

nonlimiting concentrations of all four nucleotide triphosphates can add a randomized sequence to a segment. Sequence variability in the coding nucleic acids can also be achieved by employing size-selected fragments of partially digested (or otherwise cleaved) preparations of large, natural nucleic acids, such as genomic DNA preparations or cellular RNA preparations. In those cases in which randomized sequence is employed, it is not necessary (or possible from long randomized segments) that the test mixture contains all possible variant sequences. It will generally be preferred that the test mixture contain as large a number of possible sequence variants as is

practical for selection, to insure that a maximum number of potential amino acid sequences of the translated polypeptide are identified. A

randomized sequence of 60 nucleotides will contain a calculated 10³⁶ different candidate nucleic acid sequences which would encode 10²⁶ possible

decapeptides. As a practical matter, it is possible to sample only about 10 ¹⁸ polypeptide candidates in a single selection. Therefore, candidate mRNA mixtures that have randomized segments longer than 60 contain too many possible sequences for all to be sampled in one selection. Many epitopes recognized by antibodies are only 5-10 amino acids in length. It is not necessary to sample all possible sequences of a candidate mixture to select a polypeptide ligand of the invention. It is basic to the method that the coding nucleic acids of the test mixture are capable of being amplified. Thus, it is preferred that any conserved regions employed in the test nucleic acids do not contain sequences which interfere with amplification.

The complex of a ribosome, mRNA, and nascent polypeptide attached to a tRNA in the P-site of the ribosome is very stable. Release of the nascent peptide from the complex and of the mRNA from the ribosome requires protein release factors. Release factor recognition requires the positioning of the stop codons of the mRNA in the A-site of the ribosome. In the absence of a stop codon or release factor the dissociation of the translation complex from mRNA is very slow. The addition of the antibiotics cycloheximide

(eukaryotic systems) and chloramphenicol

(prokaryotic system) further stabilizes the complexes so that extensive manipulations like column chromatography and gradient centrifugation can be performed.

For this invention, a ribosome is preferably paused at the end of a coding sequence on a mRNA with the encoded nascent polypeptide available for partitioning of the complex. There are a number of ways in which this can be

accomplished. Because stop codons are essential for release factor action, a translating ribosome that does not encounter any stop codons will proceed to the end of a mRNA and stall at the 3' end (Connolly and Gilmore, supra). In vitro

translation systems which have been depleted of release factor (by immunomactivation or mutation) will result in the stalling of translation

complexes at stop codons. Removal of GTP, the use of non-hydrolyzable analogues, and the use of certain antibiotics will also stall translational complexes. The timed addition of these exogenous factors to a synchronous in vitro translation reaction can produce predictable sizes of nascent polypeptide for the successful partitioning of the translational complex. In some organisms there exist temperature-sensitive tRNA synthetase mutants. Another way of stalling translational complexes at defined sites is to include at the 3' end of the coding region a stretch of sense codons which are recognized by a single species of tRNA for which there exists a conditional tRNA

synthetase mutant. In vitro translation reactions done from extracts of such mutants under the restrictive condition will result in stalled complexes at the stretch of sense codons for that particular tRNA.

It will be understood that it is not necessary to stall or pause the translation process to obtain partitionable ribosome

complexes. Stable complexes can be isolated at any time during active translation. It is advantageous to isolate actively translating ribosome complexes when it is desired to vary the length of the randomized segment, e.g., to test the effects of polypeptide length on binding efficacy. Ribosome complexes isolated during active translation constitute a population of nascent peptides of varied length. By

synchronously initiating translation and isolating ribosome complexes at various times thereafter, the effects of increasing polypeptide length can be compared.

Polymerase chain reaction (PCR) is an exemplary method for amplifying nucleic acids.

Descriptions of PCR methods are found, for example in Saiki et al. (1985) Science 230:1350-1354;

Saiki et al. (1986) Nature 324: 163-166; Scharf et al. (1986) Science 233:1076-1078; Innis et al.

(1988) Proc. Natl. Acad. Sci. 85: 9436-9440; and in U.S. Patent 4,683,195 (Mullis et al.) and U.S. Patent 4,683,202 (Mullis et al.). In its basic form, PCR amplification involves repeated cycles of replication of a desired single-stranded DNA (or cDNA copy of an RNA) employing specific oligonucleotide primers complementary to the 3' ends of both strands, primer extension with a DNA polymerase, and DNA denaturation. Products generated by extension from one primer serve as templates for extension from the other primer. A related amplification method described in PCT published application WO 89/01050 (Burg et al.) requires the presence or introduction of a

promoter sequence upstream of the sequence to be amplified, to give a double-stranded intermediate. Multiple RNA copies of the double-stranded

promoter-containing intermediate are then produced using RNA polymerase. The resultant RNA copies are treated with reverse transcriptase to produce additional double-stranded promoter containing intermediates which can them be subject to another round of amplification with RNA polymerase.

Alternative methods of amplification include among others cloning of selected DNAs or cDNA copies of selected RNAs into an appropriate vector and introduction of that vector into a host organism where the vector and the cloned DNAs are

replicated and thus amplified (Guatelli, J.C. et al. (1990) Proc. Natl. Acad. Sci. 87.:1874). In general, any means that will allow faithful, efficient amplification of selected nucleic acid sequences can be employed in the method of the present invention. It is only necessary that the proportionate representations of sequences after amplification reflect the relative proportions of sequences in the mixture before amplification.

Specific embodiments of the present invention for amplifying RNAs are based on Innis et al. (1988) supra. The RNA molecules in the test mixture are designed to contain a sequence transcribed from a T7 promoter in their 5' portions. Full-length cDNA copies of selected mRNA molecules are made using reverse

transcriptase primed with an oligomer

complementary to the 3 ' sequences of the selected RNAs. The resultant cDNAs are amplified by Tag DNA polymerase chain extension, employing a primer containing the T7 promoter sequence as well as a sequence complementary to the conserved 5' and of the selected RNAs. Double-stranded products of this amplification process are then transcribed in vitro. Transcripts are used in the next

selection/ amplification cycle. The method can optionally include appropriate nucleic acid purification steps.

In general, any protocol which will allow selection of polypeptides based on their ability to bind specifically to another molecule, i.e., a protein or any target molecule, can be employed in the method of the present invention. It is only necessary that the ribosome complexes be partitioned without disruption such that the selected coding mRNA's are capable of being amplified. For example, in a column binding selection in which a test mixture of ribosome complexes bearing nascent randomized polypeptide is passed over a column of immobilized target molecules, the complexes bearing polypeptide ligands of the target are retained and the non-target binding complexes are eluted from the column with appropriate buffer. A wide variety of affinity chromatography techniques, including support matrices and coupling reactions is

available for application of a column partitioning system. Target binding polypeptides together with mRNA's encoding each remain bound to the column. The relative concentrations of protein to test polypeptides in the incubated mixture influences the strength of binding that is selected for.

When polypeptide is in excess, competition for available binding sites occurs and those

polypeptides which bind most strongly are

selected. Conversely, when an excess of target is employed, it is expected that any polypeptide that binds to the target will be selected. The

relative concentrations of target to polypeptide employed to achieve the desired selection will depend on the type of target, the strength of the binding interaction and the level of any

background binding that is present. The relative concentrations needed to achieve the desired partitioning result can be readily determined empirically without undue experimentation.

Similarly, it may be necessary to optimize the column elution procedure to minimize background binding. Again such optimization of the elution procedures is within the skill of the ordinary artisan.

An unexpected feature of the invention is the fact that the polypeptide ligand need not be elutable from the target to be selectable.

This is because it is the mRNA that is recovered for further amplification or cloning, not the polypeptide itself. It is known that some

affinity columns can bind the most avid ligands so tightly as to be very difficult to elute. However the method of the invention can be successfully practiced to yield avid ligands, even covalent binding ligands. The ribosome complexes can be disrupted by denaturing agents such as urea or sodium dodecyl sulfate without affecting the integrity of the mRNA. The mRNA's of selected ligands are amplified, as described elsewhere herein, to yield a mixture of coding sequences enriched for those that encode polypeptide ligands of the desired target, including ligands that bind tightly, irreversibly or covalently.

Immunoreactivity of nascent polypeptides on ribosome complexes can be used to purify the encoding mRNAs. In the simplest application, ribosome complexes are purified from cells in the presence of inhibitors such as chloramphenicol or cycloheximide which stall translational complexes on mRNA. Binding of antibodies which recognize the epitope of interest followed by binding antibodies which recognize those antibodies results in immunoprecipitation of the ribosome complexes containing the mRNAs which encode the epitope. The background of mRNAs which do not encode the epitope of interest but are trapped by the immunoprecipitated complex can be lowered by using purified IgGs against the epitope followed by purification of the immunoreactive ribosomes on a protein A column. (IgGs are one class of the soluble immunoglobulins which compose antisera. Protein A is derived from Staphylococcus aureus and has a high affinity for IgGs. Protein A binding does not interfere with epitope

recognition.)

These procedures for immunoprecipitation to partition ribosome complexes can be used in a variety of modifications to partition the

translational complexes in SPERT. One such modification is termed "panhandling" (See Figure 4). A protein is composed of an immunoreactive domain for which known antibody exists, and a separate target domain for which one wishes to evolve protein ligands. Ribosome complexes which interact with the target domain (the "pan") via their nascent polypeptides will be

immunoprecipitated upon binding antibodies which recognize the immunoreactive domain (the

"handle"). This modification is especially useful for developing polypeptide ligands against a segment of a fusion protein in which the amino terminus is the fragment of a common protein

(beta-galactosidase, for example) and the

carboxyl-terminal portion is the protein of interest. It will also be useful for the

development of polypeptide ligands which recognize immunoresistant domains of a protein which has an immuno-dominant domain for which polyclonal sera is available. Where immunoprecipitation is employed, it will be advantageous to discard any ribosome complexes that react directly with the antibodies, prior to selection.

Alternative partitioning protocols for separating polypeptides bound to targets,

particularly proteins, are available to the art. For example, binding and partitioning can be achieved by immunoprecipitation of the test ribosome complex mixture and passing the immune complexes through a protein A affinity column which retains the immune reactive polypeptide-containing complexes as the column. Those mRNA's that encode a polypeptide that binds to the target antibody will be retained on the column as part of the ribosome complex and unbound coding mRNA's can be washed from the column.

Effective partitioning can be carried out with pure or impure target preparations. In cases where target preparations are impure, selectivity can be enhanced by strategies that enhance the binding of ligands to the desired target, or which specifically elute desired ligands or prevent their binding. The latter approach is subtractive. A known ligand can block binding of any polypeptide that can bind the target so that the desired polypeptide is

partitioned by elution and unwanted polypeptides are retained on the column.

Optionally, chemical or enzymic modifications of the polypeptide can be introduced post-translationally. The process for making such modifications should not disrupt the ribosome complexes. An important type of post-translational modification is oxidation to form disulfides in sequences that contain two or more cysteines. Particularly for small polypeptides, disulfide bonds are especially advantageous to lock in a desired conformational state so that a rigid structure having high specificity and binding affinity for a target can be achieved.

(See, e.g., Olivera, B. M. , et al. (1990) Science 249:257-263.

Other forms of post-translational structure modifications include introducing factors that non-covalently influence tertiary structure of the nascent polypeptide. In

particular, metal ions such as Ca⁺⁺, Mg⁺⁺, Mn⁺⁺, Zn⁺⁺, Fe⁺⁺, Fe⁺⁺⁺, and Mo⁶ can affect polypeptide folding configuration by forming coordination complexes with amino acid side chains. Similarly organic compounds such as nicotinamide

nucleotides, flavine nucleotides, porphyrins, thiamine phosphates, serotonin, and the like, including inhibitors, agonists and antagonists of known biological functions, can interact with the nascent polypeptide to modify its 3-dimensional folded configuration. As thus modified, the nascent polypeptide can exhibit different binding properties than an unmodified polypeptide. The use of such configurational modifiers enhances the range of potential binding activities of any candidate mixture of polypeptides. Also, it affords a means for selecting polypeptides having conditionally reversible functions, i.e., capable of being functionally "off" or "on", depending on the presence or absence of the modifier.

Configurational modifiers need not be naturally-occurring compounds. The use of such modifiers during partitioning is only limited by the need to maintain stability of the ribosome complexes.

Modifiers which disrupt ribosome complexes or which degrade the coding mRNA or nascent

polypeptide should be avoided. A modifier can be included in the buffer or medium during

partitioning. Alternatively, SPERT itself can be used to pre-select polypeptides which bind the modifier as a target after which the candidate mixture of selected modifier-binding polypeptides can be further selected, via SPERT, for binding the ultimate target.

Sequence variation in the test coding mRNA mixture can be achieved or increased by mutation. For example, a procedure has been described for efficiently mutagenizing nucleic acid sequences during PCR amplification (Leung et al. 1989). This method or functionally equivalent methods can optionally be combined with

amplification procedures in the present invention.

Alternatively, conventional methods of DNA mutagenesis can be incorporated into the nucleic acid amplification procedure. Applicable mutagenesis procedures include, among others, chemically induced mutagenesis and oligonucleotide site-directed mutagenesis.

The starting mRNA mixture is not limited to sequences synthesized de novo. In particular, SPERT can be used to modify the function of existing proteins. A segment of the natural sequence is replaced by a corresponding segment of randomized sequence in the mRNA that encodes the protein. Since many known proteins belong to families having some sequences conserved and others varied, the logical approach is to replace the variable (or hypervariable) regions with randomized sequence, to maximize the chance of altering function. The proper choice of

partitioning conditions, as will be apparent to those skilled in the art, results in selection for the desired functional variant. In this way, modifications, alterations and improvements on known proteins can be achieved.

In order to proceed to the amplification step, coding nucleic acids must be released from the target-bound ribosome complexes after

partitioning. This process must be done without chemical degradation of the coding mRNA's and must result in amplifiable nucleic acids. In a

specific embodiment, selected coding RNA molecules are eluted from a column using a high ionic strength buffer or other eluant capable of

disrupting the ligand-target bond. Alternatively, the ribosome can be denatured such that the mRNA is eluted. The coding mRNA can be removed from ribosome complexes or from ribosome complex-target pairs by phenol extraction or by phenol combined with a protein denaturing agent such as 7M urea. Although ribosomal RNA is also extracted,

subsequent amplification is selective for the mRNA's because the primers used for cDNA synthesis and PCR amplification are complementary only to a conserved sequence in the mRNA's and not to ribosomal RNA.

As the translation of randomized mRNAs proceeds during the SPERT protocol, the growing polypeptide makes its way from the peptidyl transferase site within the large ribosome subunit toward the cytoplasmic solvent. The peptidyl transferase site is an intrinsic activity of the large ribosome subunit from all organisms; that site has been defined functionally but its precise location within the ribosome is unknown. However, the distance between that site and the cytoplasmic solvent also is known to be about 30 to 40 amino acids in length.

For optimal effectiveness in SPERT, the random portion of the nascent polypeptide (whose properties are selected during the procedure) should be "outside" the ribosome in order for partitioning of the ribosome complex to fully utilize the properties of the randomized

polypeptide. A C-terminal trailer sequence is preferably incorporated into the translated polypeptide to insure that the randomized sequence is fully exposed after translation. From the work of Smith et al, (PNAS, 75:5922, 1978) and Malkin and Rich (J. Mol. Biol., 26:329, 1967) for both prokaryotes and eukaryotes: about 30 to 40 amino acid residues remain within the ribosome during translation. Furthermore, if the amino-terminus of a growing polypeptide contains a hydrophobic domain of about 20 amino acid residues, a nascent polypeptide of about 50 residues has been shown to be enough to allow the translation complex to interact with a membrane by hydrophobic

interactions, see Kurzchalia et al, Nature

320:634, 1986). Thus, in preferred embodiments of SPERT, the randomized polypeptide will be encoded by randomized mRNA that is about 30-40 codons

(that is, about 90-120 nucleotides) upstream from the codons at which the translation complex stalls. It will be understood that both longer and shorter C-terminal trailer sequences can be used effectively, and that SPERT, itself, can be used to determine optimum trailer length for a given partitioning system. The sequence of mRNA and encoded polypeptide in the C-terminal trailer can be designed to have any other desired function, such as more stability in the

translation complex, ease of in vitro

manipulation, subsequent pc ypeptide purification, as a reporter activity for diagnostics, cell entry, etc.

Polypeptides selected by SPERT can be produced by any peptide synthetic method desired. Chemical synthesis can be accomplished since the amino acid sequence of the polypeptide is readily obtainable from the nucleotide sequence of the coding mRNA. Since cDNA from the coding mRNA is available, the polypeptide can also be made by expressing the cDNA in a suitable host cell.

It is an important and unexpected aspect of the present invention that the methods

described herein can be employed to identify, isolate or produce polypeptide molecules which will bind specifically to any desired target molecule. Thus, the present methods can be employed to produce polypeptides specific for binding to a particular target.

Proteins contain within their primary sequence the information required to form an extraordinary variety of three dimensional shapes as is well known in the art. From this variety of potential shapes, along with the charge and/or hydrophobic qualities of amino acids, comes the potential for protein functions that are used in the biosphere. Proteins provide catalysis when embodied as enzymes; proteins can provide stable biological structures, for example, when used to construct spores, membranes, or viruses; and proteins can provide binding to a variety of targets, with appropriate affinities and kinetic parameters to allow life.

Nevertheless, this vast potential in chemical activities, including the extreme

potential inherent in the mammalian immune system, has actually been explored rather narrowly by organisms. This fact can be noted with a simple calculation. If the average length of a protein is 300 amino acids, and if there are twenty natural amino acids used to construct modern proteins, the number of possible sequences of proteins of average size is 20³⁰⁰ or ~10⁴⁰⁰.

Estimates of the number of particles in the uni •verse are in the range 1080, while esti•mates for the number of proteins ever explored in the entire hi •story of the earth are in the range 1010. The tiny fraction of so-called sequence space that has been explored by biology is a result of

evolutionary history and the relatively short age of the earth. The present invention provides the means to explore protein sequence space without historial and evolutionary limitations, while continuing to respect limitations established by the number of particles in the universe. The invention provides the means to identify and isolate polypeptide ligands with any desired quality from vast mixtures of protein sequences comprised largely of individual entities that have never before existed. The amino acid sequence of the selected ligand can be learned from the nucleotide sequence of its encoding mRNA, making tedious amino acid sequence determination

unnecessary.

Even where the binding functions selected by SPERT have known naturally occurring counterparts, there is no reason to expect that the polypeptides selected by SPERT will resemble naturally-occurring proteins or peptides having similar function. In most instances, SPERT-selected polypeptides will be smaller than

naturally-occurring proteins typically having a size of from 4-100 amino acids, preferably from 4-50 amino acids selected from randomized sequence of the same length, and also having a C-terminal trailer of about 30-40 amino acids and, optionally a N-terminal leader of about 10 amino acids, for a total length of about 100 amino acids,

corresponding to a molecular weight of about llkd. This is smaller than most enzymes and all

antibodies, for comparison, IgG has a molecular weight of about 150kd. Furthermore, many

polypeptide ligands of the invention will function when freed by N- and C- terminal trailers.

Therefore, the final product can be as small as 4-50 amino acids. The polypeptides of the invention are non-naturally-occurring, and typically differ in amino acid sequence and molecular size from naturally-occurring proteins. That portion of the amino acid sequence arising from randomized coding is designated the "binding segment" herein. The binding segment can be of any length, conveniently ranging from about 4-100 amino acids in length, preferably from about 15-50 amino acids in length. Additionally, given the vastness of sequence space, it is expected that most polypeptide ligands of the invention will have less than 50% homology with natural proteins, and preferably less than 30% amino acid homology with natural proteins. A polypeptide ligand of the invention in a number of ways functionally resembles an

antibody. Polypeptide ligands which have binding functions similar to those of antibodies can be isolated by the methods of the present invention. Such polypeptides are generally useful in

applications in which polyclonal or monoclonal antibodies have found application. However, the polypeptide ligands of the invention have

significant advantages over antibodies: they can be selected for any desired affinity, including higher affinities than are obtainable with

antibodies, they can be selected to bind at any desired epitope or combination of epitopes, including binding sites not recognized by

antibodies, they can be larger or smaller and have different solubility properties than antibodies and they can be generated by techniques that operate entirely in vitro, without the need for live animals or cell culture techniques.

Applications of polypeptide ligands include the specific, qualitative or quantitative detection of target molecules from any source; purification of target molecules based on their specific binding to the polypeptide; and various therapeutic methods which rely on the specific direction of a toxin or other therapeutic agent to a specific target site. Target molecules are preferably proteins, but can also include among others carbohydrates, nucleic acids, peptidoglycans and a variety of small molecules. As with conventional antibodies, polypeptide ligands can be employed to target biological structures, such as cell

surfaces or viruses, through specific interaction with a molecule that is an integral part of that biological structure. Polypeptide ligands are advantageous in that they are not limited by self tolerance, as are conventional antibodies. Also, as noted, polypeptide ligands of the invention do not require animals or cell cultures for synthesis or production, since SPERT is a wholly in vitro process. The methods of the present invention related to the use of polypeptide ligands can generate novel polypeptides that bind targets for which other proteinaceous ligands are known. For example, a number of proteins are known to

function via binding to nucleic acid sequences, such as regulatory proteins which bind to nucleic acid operator sequences. The known ability of certain nucleic acid binding proteins to bind to their natural sites, for example, has been

employed in the detection, quantitation, isolation and purification of such proteins. The methods of the present invention related to the use of polypeptide ligands can be used to make novel nucleic acid binding ligands having affinity for nucleic acid sequences which are known to bind proteins and to nucleic acid sequences not known to bind proteins. Novel, non-naturally-occurring polypeptides which bind to the same binding sites of nucleic acids can be developed using SPERT. As will be discussed below, certain polypeptides isolatable by SPERT can also be employed to affect the function, (for example inhibit, enhance or activate) specific target molecules or structures. Specifically, polypeptide ligands can be employed to inhibit, enhance or activate the function of proteins and of nucleic acids. It is a second important aspect of the present invention that the methods described herein can be employed to identify, isolate or produce polypeptide molecules which will bind specifically to a particular target molecule and affect the function of that molecule. In this aspect, the target molecules are again preferably proteins or nucleic acids, but can also include, among others, carbohydrates and various small molecules to which specific polypeptide binding can be achieved. Polypeptide ligands that bind to small molecules can affect their function by sequestering them or by preventing them from interacting with their natural ligands. For example, the activity of an enzyme can be affected by a polypeptide ligand that binds the enzyme's substrate. Polypeptide ligands of small molecules are particularly useful as reagents for diagnostic tests, or other quantitative assays. For example, the presence of controlled substances, bound metabolites or abnormal quantities of normal metabolites can be detected and measured using polypeptide ligands of the invention. Antibodies to polypeptide ligands can be used to precipitate or bind ligand-target pairs to a solid phase matrix in a diagnostic assay. A polypeptide ligand having catalytic activity can affect the function of a small molecule by catalyzing a chemical change in the target. The range of possible catalytic activities is at least as broad as that displayed by natural proteins.

The strategy of selecting a ligand for a transition state analog of a desired reaction is one method by which catalytic polypeptide ligands can be selected. Polypeptide ligands with high affinity for transition-state analogues are likely to have enzymatic activity, as has been

demonstrated for monoclonal antibodies directed against transition-state analogues. These

antibodies have exhibited a wide range of

catalytic activities, including acyl-transfer reactions [Pollack et al., Science 234:1570

(1986); Tramantano et al., Science 234:1570

(1986); Jacobs et al., J. Am. Chem. Soc. 109:2174 (1987); Napper et al., Science 237:1041 (1987); Janda et al., Science 241:1188 (1988); Schultz, P.G., Science 240:426 (1988); Benkovic et al., Proc. Natl. Acad. Sci. 85:5355 (1988)], carbon-carbon bond formation [Jackson et al., J. Am.

Chem. Soc. 110:4841 (1988); Hilvert and Nared, J. Am. Chem. Soc. 110:5593 (1988)], carbon-carbon bond cleaving reactions [Cochran et al., J. Am. Chem. Soc. 110:7888 (1988)], peptide cleavage

[Iverson and Lerner, Science 243: 1184 (1989)], and ester bond hydrolysis [Janda et al., Science

244:437 (1989)]. The number of polypeptide sequences and structures that can be explored by SPERT far exceed those available in the immune system.

Enzymes are evolved using SPERT and starting randomized sequences corresponding to about 50 amino acids, as in Example 3. Enzymatic polypeptide ligands of small size are entirely unanticipated by the present understanding of enzymology; enzymes are always much larger in nature than the scientist expects. The specific transition state analogues used are drawn from the literature cited above. Among the reactions probed by the monoclonal antibody-enzymes are some which lead to the breakdown of toxic waste

products, including chemicals with chlorine-carbon bonds and carbon-carbon bonds in ring structures like those found in benzene and polychlorinated phenols.

The binding selection methods of the present invention can be combined with secondary selection or screening to identify ligands capable of modifying target molecule function upon

binding. The large population of variant amino acid sequences that can be tested by SPERT

enhances the probability that polypeptide

sequences can be found that have a desired binding capability and that function to modify target molecule activity. The methods of the present invention are useful for selecting polypeptide ligands which can selectively affect function of any target protein. The methods described herein can be employed to isolate or produce polypeptide ligands which bind to and modify the function of any protein or nucleic acid. It is contemplated that the method of the present invention can be employed to identify, isolate or produce

polypeptide molecules which will affect catalytic activity of target enzymes, i.e., inhibit

catalysis or modify substrate binding, affect the functionality of protein receptors, i.e., inhibit binding to receptors or modify the specificity of binding to receptors; affect the formation of protein multimers, i.e., disrupt quaternary structure of protein subunits; and modify

transport properties of protein, i.e., disrupt transport of small molecules or ions by proteins. Secondary selection methods that can be combined with SPERT include among others

selections or screens for enzyme inhibition, alteration of substrate binding, loss of

functionality, disruption of structure, etc.

Those of ordinary skill in the art are able to select among various alternatives those selection or screening methods that are compatible with the methods described herein.

An embodiment of the present invention, which is particularly useful for identifying or isolating polypeptides which bind to a particular functional or active site in a protein, or other target molecule, employs a molecule known, or selected, for binding to a desired site within the target protein to direct the

selection/amplification process to a subset of polypeptide ligands that bind at or near the desired site within the target molecule. In a simple example, a polypeptide sequence known to bind to a desired site in a target molecule is incorporated near the randomized region of all polypeptides being tested for binding. SPERT is then used to select those variants, all of which will contain the known binding sequence, which bind most strongly to the target molecule. A longer binding sequence, which is anticipated to either bind more strongly to the target molecule or more specifically to the target can thus be selected. The longer binding sequence can then be introduced near the randomized region of the poxypeptide test mixture and the

selection/amplification steps repeated to select an even longer binding sequence. Iteration of these steps (i.e., incorporation of selected sequence into test mixtures followed by

selection/amplification for improved or more specific binding) can be repeated until a desired level of binding strength or specificity is achieved. This iterative "walking" procedure allows the selection of polypeptides highly specific for a particular target molecule or site within a target molecule. Another embodiment of such an iterative "walking" procedure, employs an "anchor" molecule which is not necessarily a polypeptide or amino acid. In this embodiment a molecule which binds to a desired target, for example a substrate or inhibitor of a target enzyme, is chemically modified such that it can be covalently linked to a bridge molecule which in turn is known to be bound to an oligopeptide of known sequence. The bridge molecule covalently linked to the "anchor" molecule that binds to the target also binds to the target molecule. The sequence encoding the known bridge-binding

oligopeptide is incorporated near the randomized region of the test nucleic acid mixture. SPERT is then performed to select for those polypeptide sequences that bind most strongly to the target molecule/bridge/anchor complex. The iterative walking procedure can then be employed to select or produce longer and longer polypeptide molecules with enhanced strength of binding or specificity of binding to the target. The use of the "anchor" procedure is expected to allow more rapid

isolation of polypeptide ligands that bind at or near a desired site within a target molecule. In particular, it is expected that the "anchor" method in combination with iterative "walking" procedures will result in polypeptides which are highly specific inhibitors of protein function.

In accordance with the teachings of copending application Serial No. 07/536,428, the translated mRNA of a ribosome complex is, in principle, capable of binding to target molecules and of being partitioned concurrently with nascent polypeptides. In particular, where partitioning is accomplished by affinity chromatography, the selected ligand can be an RNA, rather than a polypeptide. Binding of mRNA can be

differentiated from polypeptide binding once the ligand has been selected and both the selected polypeptide and its coding mRNA are available for independent direct binding studies where the two are not part of the same ribosome complex.

Comparative studies of the relative frequency of RNA ligands and polypeptide ligands selected by SPERT are of fundamental biological importance to understanding the specialization of function that currently exists in living cells.

The polypeptides of the invention can be selected for other properties in addition to binding. For example, during partitioning, stability to certain conditions of the desired working environment of the end product can be included as a selection criterion. If a

polypeptide which is stable in the presence of a certain protease is desired, that protease can be part of the buffer medium used during

part: ioning As will be understood, conditions which disruptionibosome complexes should be

avoided. Other desired properties can be incorporated, directly into the polypeptide sequence as will be understood by those skilled in the art. For example, membrane affinity can be included as a property, either by employing a N- or C-terminal trailer having high hydrophobicity, or by biasing the randomized coding to favor the amino acids with lipophilic side chains.

The coding nucleic acid concomitantly selected by partitioning nascent polypeptides as described, is useful in its own right to transform host cells or organisms. The transformed organism is then useful for, e.g., fermentation production of the selected polypeptide. A transgenic organism can be rendered resistent to a virus infection, for example, by causing in vivo synthesis of a polypeptide ligand of the viral nucleic acid or a key viral protein. In principle, any functionality contributed by a polypeptide ligand of the invention can be bestowed on a suitable host organism. Methods known in the art can be used to combine the coding region with a promoter,

polyadenylation signal functional in the intended host, followed by incorporation into a suitable vector for transformation, all as known and understood in the art.

Table 1

1. -CCGAAGCTTAATACGACTCACTATAGGGCGACATACATTTACACACATAA-3' 2. -CGGGAATTCTTTCATATTATATTTCCTCCTTATGTGTGTAAATGTATG-3 ' 3. -GGCGAATTCTGCTGCTGCAGTGCTGCCATGGTTGCGACGGTCAGGA-3 ' 4. -CCGCCGGATCCTCCTGTCCGTCGCAA-3'

5. -CCCGAATTC-[-45N-]-CTGCAGTGCTGCCATGGT-3'

6. -ACCATGGCAGCACTG-3'

7. -GGGCCATGG- [-120(ACG)-]-CCATGGTTGCGATGGTCAGGA-3'

8. -TCCTGTCCATCGCAA-3'

EXAMPLES

The techniques and methods used in the ensuing examples are published and known in the art. Together with adaptations and modifications known to those of ordinary skill in the art, the procedures not specifically referenced herein are available from known reference works. In addition to Sambrook et al., (1989) supra, Genetic

Engineering, Plenum Press, New York (1979); Weir, (ed.) (1986) Handbook of Experimental Immunology in Four Volumes, 4th Ed, Blackwell Scientific Publications, Oxford; and the multivolume Methods in Enzymology published by Academic Press, New York. Polymerase chain reaction techniques are described in PCR Protocols (Michael A. Innis, et al. eds.) (1990) Academic Press, Inc.

Throughout the following examples, reference is made to Tables l and 2. Table 1 lists oligonucleotide sequences used for preparing mRNA candidates. Table 2 lists the same sequences together with explanatory notes showing functional domains. Sequences in capitals are chemically synthesized, sequences in lower case letters are complementary sequences made enzymatically by DNA polymerase.

Example 1. Direct Immunoprecipitation of

Ribosome Complexes: Polypeptide Ligands Directed Toward

Immunoglobulin Molecules.

The method of the invention is used to select novel polypeptides that bind the antibody of an epitope commonly recognized by the antisera from autoimmune mice which are the fl progeny of a cross of NZB and NZW parents (Portanova et al., J. Immunol. 144, 4633 (1990). The known epitope consists of about 10 contiguous amino acids at the amino terminus of the histone H2B protein. To make mRNA encoding candidate polypeptides, a 5' fixed sequence composed of a T7 promoter sequence and a ribosome binding site which is recognized by both prokaryotic and eukaryotic ribosomes,

terminating in a restriction endonuclease site is synthesized and cloned using oligonucleotides having the sequences shown as sequence 1 in Tables 1 and 2 and in Figure 8. A 3' fixed sequence is placed into a restriction site to provide an mRNA encoding the C-terminal trailer sequence of ca.

100 nucleotides lacking stop codons (for ca. 30-35 amino acids) shown as sequence 3 in Tables 1 and 2 and Figure 8. In addition, as shown in Figure 1, a 3' primer annealing site (sequence 3) is

provided so that cDNA synthesis can be

accomplished on the mRNA recovered from

partitioned ribosome complexes.

The randomized polypeptide insertion site is bounded by restriction endonuclease recognition sites, in this example EcoRI and PstI. A single-stranded oligonucleotide is synthesized with a randomized sequence of 45 nucleotides

(corresponding to 15 codons) bounded by specific sequences that include those two restriction endonuclease sites (Sequence 4a). Synthesis of randomized oligonucleotides is carried out using an Applied Biosystems DNA synthesizer provided with a reactant mixture for each nucleotide position. To partially compensate for the amino acid sequence bias inherent in the redundancy of the genetic code, the reaction mixtures contain, on a mole percent basis, the following composition of bases for each codon: First position, C-20%, T, A, and G-30% each; Second position, C-15%, A- 35%, T and G-25% each; Third position, T, C, A and G-25% each. Using a nucleic acid primer that is complementary to the fixed 3' end of the

randomized oligonucleotide, randomized double-stranded DNA is created with the action of DNA polymerase. The products are digested with the two restriction endonucleases and ligated between the 5' fixed sequence and the 3' fixed sequence discussed above, in vitro transcription of these ligated templates using T7 RNA polymerase

(Bethesda Research Laboratories, Gaithersburg, MD) provides mRNA templates for in vitro translation. A rabbit reticulocyte lysate system (BRL) is used to translate the mRNA templates in vitro, using standard reaction conditions. Such translation of these transcripts results in a variety of

ribosomal complexes (mRNA-nascent polypeptide-tRNA-ribosomes) that are identical except for the randomized region of the nascent polypeptide.

Antibodies (IgGs), Portamova et al..

supra, which recognize the H2B histone epitope are added to the in vitro translation mixture.

Immunoprecipitation of the immunoreactive ribosome complexes partitions the mRNAs species that encode the highest-affinity polypeptide ligands in the population (see Figures 3 and 4).

Immunoprecipitated complexes are separated by low speed centrifugation. cDNA is synthesized from these mRNAs and is used via PCR to provide template for further cycles of transcription, translation, immunoselection and cDNA synthesis.

Clones are isolated as described in Application 07/536,428, June 11, 1990,

incorporated herein by reference. The individual polypeptide products are over produced and

purified and tested, using standard techniques for reactivity to the anti-H2B histone antibodies. In addition, the polypeptide ligands are challenged competitively with authentic histone H2B-derived epitopes to discover which polypeptide ligands bind to the same portion of the antibodies as the true epitope. Among the polypeptides isolated that bind the antibody are found those having less than 50% sequence homology with the H2B histone epitope. Other antibody binding sequences are identified having less than 30% homology with the H2B histone epitope. Other polypeptide ligands of the antibody do not compete for the H2B epitope binding site.

Example 2. Diagnostics using the polypeptide ligands of Example 1: An assay for anti-H2B antibodies in the progeny of NZB X NZW mice.

Auto-immune diseases result from the elaboration of an inappropriate antibody molecule with reactivity toward a normal cellular component (often a protein, but sometimes a nucleic acid, as in Systemic Lupus Erythematosis - SLE).

Polypeptide ligands generated through the SPERT protocols in Example 1 are aimed at diagnosis of mouse "Lupus" in the offspring of NZB X NZW mice. SPERT is used to identify and obtain a reagent ligand for the diagnostic recognition of the auto-antibody that recognizes the histone H2B epitope.

As in Example 1, ribosome complexes are treated with the auto-antibody to partition reactive polypeptides from non-reactive

polypeptides resident (as nascent polypeptides) in ribosome complexes. The auto-antibodies are used to precipitate the ribosome complexes containing polypeptides that fit into the active site of the antibody. The most avidly bound polypeptide emerges from repeated SPERT cycles.

The most avidly bound polypeptide ligand does not resemble in detail the epitope identified as the portion of the target that reacts with the antibody. Auto-immune diseases are triggered by unknown antigens, which are not necessarily the same as the target/epitope identified as the interactive species during the clinical stage of the auto-immune disease. For example, a virus infection may trigger an immune reaction that yields a class of antibodies that cross-react with a normal cellular target. Such antibodies may bind more avidly to the original, stimulatory, viral antigen than to the epitope on the cellular target. As another example, the epitope on the cellular target may not take full advantage of the binding site on the antibody.

The polypeptide ligand is used diagnostically to measure the quantity of

circulating auto-antibody, using, e.g., an ELISA assay. The technology is available to one skilled in the art, without undue experimentation. As another example, the fixed portion of the polypeptide ligand is used as the reporter substance when the polypeptide ligand interacts with the circulating auto-antibody. With a fixed carboxy-terminus of beta-galactosidase or alkaline phosphatase, serum protein samples attached to plastic plates are assayed directly for the anti-H2B antibody by "staining" with the polypeptide ligand covalently fused (by recombinant DNA techniques) to either reporter enzyme.

Example 3. Indirect Immunoprecipitation:

Polypeptide ligands directed toward domains of any protein. Immunization of animals with antigens, whether crudely prepared or purified, often results in immune responses directed at a subset of the available epitopes in that antigen. The polyclonal sera may react largely with a single protein domain in that antigen. Similarly, when researchers attempt to raise antibodies against fusion proteins, often the well-known fusion partner is immuno-dominant over the new protein portion of the fusion.

Antibodies aimed at a protein target

(but that do not recognize the portion of the target that one wishes to use as the target in SPERT) allow INDIRECT Immunoprecipitation of ribosome complexes. That is, immunoprecipitation is a useful partitioning step when antibodies are aimed at domains in the target that are different from those domains pre-selected for SPERT-based ligand evolution. This protocol is sometimes called "panhandling", and can yield high-affinity polypeptide ligands for target domains that are weakly immunogenic.

SPERT. is performed using variable material prepared as in Example 1 except that the randomized mRNA regions are now set to yield about 50 amino acids in the solvent-exposed nascent polypeptide. Biased randomization is done so that chain termination codons are not likely over the 150 randomized nucleotides; in addition, cell-free translation is performed in the presence of socalled suppressor tRNAs so that translation continues to the desired portion of the mRNAs.

The population of ribosome complexes is pre-treated with the antisera aimed at the target protein, but in the absence of that target

protein. The pre-treatment is designed to

eliminate any nascent polypeptides that react directly with the antibodies, as in Example 1.

The target protein is then added to the ribosome complexes, along with antibodies aimed at the target protein. Partitioning occurs as the ribosome complexes that interact with the target at the same time (see Figure 4).

The single-stranded DNA binding protein of bacteriophage T4 (gp32) has an acidic

carboxyterminal region which is immunodominant (K. Krassa, Ph.D., Thesis, 1987). In one immunization experiment, polyclonal sera react exclusively with the carboxyterminal domain of the protein; 12 monoclonal cell lines derived from hybridoma fusions with 12 monoclonal cell lines derived from hybridoma fusions with spleen cells from such immunized animals produced antibodies that react with the same target domain. Purified polyclonal sera which react with the carboxy-terminal domain of gp32 are used for indirect immunoprecipitation in this example.

A population of ribosome complexes is produced (above). These ribosome complexes are pre-treated with the polyclonal sera aimed at gp32; this is readily accomplished by passing the ribosome complexes through Staph A columns prebound with the polyclonal sera against gp32.

Subsequently, those ribosome complexes unable to react directly with antibodies raised against gp32 are reacted with gp32, followed by treatment with the sera aimed at the carboxy-terminus of gp32. Goat anti-mouse antibodies are used to

immunoprecipitate gp32 and whatever ribosomal complexes interact with the core domain of gp32. Cycles of SPERT are continued until a desired level of binding is attained. Sequences are then cloned and individuals identified and tested for affinity to gp32.

Example 4. Isolation of a polypeptide ligand for a serine protease. Serine proteases are protein enzymes that catalyze hydrolysis of peptide bonds within proteins, often with high selectivity for specific protein targets (and, of course, for specific peptide bonds within the target protein). The serine proteases are members of a gene family in mammals. Examples of serine proteases are tissue plasminogen activator, trypsin, elastase,

chymotrypsin, thrombin, and plasmin. Many disease states can be treated with polypeptide ligands that bind to serine proteases, for example, disorders of blood clotting. Elastase inhibitors are likely to be useful in minimizing the clinical progression of emphysema. Proteases other than serine proteases are also important in mammalian biology, and these too are targets for polypeptide ligands with appropriate affinities obtained according to the invention herein taught.

A ligand that binds to porcine elastase is identified and purified using the starting randomized material of Example 3. Serine

proteases are easily attached by standard methods to column support materials with retention of enzymatic activity. Porcine elastase attached to agarose is available from commercial sources.

Thus, in this example affinity chromatography is the partitioning method. Natural elastase

inhibitors are available, and are used to check that the active site of the bound elastase is available for the binding of an inhibitory ligand. The buffer used for binding during the SPERT cycles must not denature or otherwise inactivate elastase; dithiothreitol, which can reduce protein disulfide bonds, is left out of the binding buffer.

After several rounds of SPERT, as the affinity of the mixture of nascent polypeptides becomes high, a reversal of the elution parameters is used. Early rounds of SPERT are aimed at obtaining any polypeptide ligand that binds to any domain of elastase; after virtually all the nascent polypeptides are able to bind the column, the ribosome complexes are poured through a column that has been pre-saturated with a natural inhibitory ligand for the elastase active site. In addition, the elution buffer for this procedure includes high concentrations of that same natural inhibitory ligand. The ribosome complexes that are not bound in this reversed elution procedure are used to prepare mRNAs for further SPERT cycles, once again depending on high affinity for the bound elastase. This procedure focuses the evolving polypeptide ligands toward the elastase active site.

When the mixture of polypeptide ligands has a high affinity for the bound elastase, and is aimed primarily toward the active site, further enrichment for high affinity inhibitors of

elastase activity is accomplished by including low concentrations of the natural inhibitors in the partitioning steps, thus demanding that the evolving polypeptide ligands have higher affinity than the effective affinity of the natural

inhibitor at the concentration used.

Nucleic acids encoding polypeptide ligands are cloned and sequenced, and binding affinities and inhibitory binding affinities for elastase are measured. Binding affinities and inhibitory efficiencies are measured with the same polypeptide ligands for other members of the serine protease family in order to ascertain specificity within the family. Example 5. Polypeptide ligands that antagonize a receptor: A synthetic inhibitor of the interleukine 1 receptor.

Receptors are a class of proteins that are partially integrated into the cell's

cytoplasmic membrane such that a domain resides outside the cell. That domain serves as a binding site for cell extrinsic molecules, including growth factors, peptide hormones, non-peptide organic molecules (which may include hormones), or even ions. Receptors handle the bound ligand in several different ways, including signal

transduction through the membrane or

internalization of the bound ligand for its subsequent function. In either case polypeptide ligands of the invention may be used to affect function of the receptor, that is to cause the normal activity of the natural ligand or to block that activity.

Receptor antagonism for a useful

therapeutic purpose is accomplished by generating a polypeptide ligand through SPERT that is aimed at the interleukine 1 (IL-1) receptor. A natural antagonist of the receptor has been found (Hannum et al., Nature, 343:336-340 (1990); Eisenberg et al., Nature, 343:341-346 (1990), and that

antagonist has the presumptive utility of

preventing or easing inflammatory problems such as those found in rheumatoid arthritis. The natural antagonist (called IL-lra for IL-1 receptor antagonist) is partially homologous to IL-1 itself, and is a competitive inhibitor of

interleukine 1 binding to the receptor. The natural IL-lra is a pure antagonist, completely without agonist activity at the highest

concentrations used in the work cited above. IL-lra is synthesized as a protein with 177 amino acids; after post-translational cleavage the active inhibitor has 152 amino acids and,

additionally, is glycosylated. However, the activity of recombinant IL-lra, without

glycosylation, is comparable to the activity of the natural inhibitor.

SPERT is used to develop a polypeptide ligand antagonist for the interleukine 1 receptor. Two methods are used. In the first monoclonal antibodies are raised against interleukine 1 that are able to cross-react with IL-lra. Such

monoclonal antibodies in principle recognize the features in common between IL-1 and IL-lra. Those monoclonal antibodies are used, as in Example 1, to develop polypeptide ligands that bind to the antigen combining site; such polypeptide ligands are candidates for a novel class of IL-1

antagonists. Since one goal in this case is to provide antagonists smaller than the natural IL-lra, the randomized polypeptide is ca. 50 amino acids, as in Example 3.

In a second methodology the

extracellular domain of the IL-1 receptor is itself used as the target for polypeptide ligand development through SPERT. the domain is attached to an insoluble matrix. Candidate polypeptide ligands, residing in ribosome complexes, are partitioned on the matrix. The matrix is eluted with high concentrations of IL-1, thus displacing the ribosome complexes and nascent polypeptides with the natural ligand known to bind to the desired active site on the receptor. Cycles of SPERT are continued until high affinity

polypeptide ligands are identified.

Very high affinity, even covalent, antagonists of the receptor are isolated by an elution protocol during SPERT that denatures the ribosome complexes even if the polypeptide ligand remains strongly bound tσ the receptor. The mRNA eluted from the column under protein denaturing conditions is used to prepare cDNA which is amplified through PCR, after which transcription provides mRNA for the next round of SPERT.

All genes encoding polypeptide ligands are sequenced, and the polypeptide ligands are tested for IL-1 receptor antagonism. Those ligands identified by receptor-based affinity chromatography are tested with the antibodies of the first method to screen for the novel

antagonists recognized by those antibodies that recognize structural or sequence homology between IL-1 and IL-lra. Novel, SPERT-generated

polypeptide ligands having IL-1 receptor

antagonist activity are isolated and

characterized. SPERT-generated antagonists having less than 50% amino acid homology with natural IL-lra are identified. In addition, SPERT-generated antagonists having less than 30% amino acid homology are identified.

Example 6. Protein improvement by SPERT:

Mutagenesis and selection of better natural insecticides. Bacillus thuriengiensis is a gram-positive, spore-forming bacteria which produces insecticidal proteins. These proteins, derived from different B . thuringiensis strains , have varying effectiveness for killing insect larvae of different species. Although one specific protein will kill the insect larvae of a variety of species, the effectiveness toward the different insect targets (measured as the level of protein required to produce 50% mortality) can vary by as much as 2000-fold. The mechanism of action for these insecticide proteins is to bind a receptor on the gut membranes of the susceptible insect larva. Such membranes serve as a functional partitioning tool in SPERT.

We create double-stranded DNA templates suitable for SPERT by PCR; the appropriate DNA encodes the N-terminal 646 amino acid portion of the insecticidal protein from t. subspecies kurstaki HD-1, which is fully active (Fischhoff et al., Biotechnology 5:807-813 (1987). This protein kills the larva of tomato hornworm and cabbage looper very effectively at low concentration.

Substantially more protein is required to kill tobacco budworm, corn earworm, black cutworm, European cornborer, and beet armyworm. Gut membranes from each of these insect larvae will be used as partitioning agents in SPERT.

The starting material in these experiments is RNA derived from the cloned gene, as above. Two methods are used to create protein variants. In one method mutagenic PCR provides random mutations throughout the 646 amino acids of the insecticide. In fixed codons within the insecticide, using about 50 amino acid

replacements. In particular, randomized DNA is used to replace the codons encoding the

hypervariable region of the Bt. toxin. Rounds of SPERT are continued until a desired level of binding to gut membranes is achieved. The DNA products are cloned and sequenced and individually assayed for effectiveness in binding membranes and larval killing. Effective toxins are selected by SPERT, having a naturally-occurring sequence replaced by a sequence that is less than 50% homologous with the replaced sequence. In

addition, toxic, SPERT-generated variants are identified wherein the original, naturally-occurring sequence is replaced by a sequence having less than 30% sequence homology with the replaced sequence.

Example 7. Anti-viral polypeptide ligands:

Inhibition of viral entry into target cells.

Receptors are often used for viral attach on cells. Recently Kaner et al. (Science, 248:1410-1413 (1990)) described the basic

fibroblast growth factor (FGF) receptor as the likely portal through which Herpes Simplex Virus Type 1 (HSV) enters a cell. In that same paper, by citation of other work several other viruses are said to utilize other receptors to gain cellular entry. Rhinovirus, the common cold virus, is said to enter cells through a cell adhesion molecule ICAM-1. HIV, the AIDS virus, enters cells through the CD4 glycoprotein

receptor. Epstein-Barr virus enters T lymphocytes via the C3d complement receptor. Rabies virus enters nerve cells through the acetylcholine receptor. Reovirus enters cells through the beta-adrenergic receptor. Vaccinia virus enters cells through a functional interaction with the

epidermal growth factor receptor. Apparently viruses survive in part by using absolutely crucial cell receptors to gain entry into

susceptible hosts. That is, host organisms can not easily alter such important receptors so as to become resistant to the virus without suffering some impairment of crucial cell and organism functions.

Polypeptide ligands of the invention are identified that diminish viral uptake through receptors while still allowing critical growth factors to function. The basic FGF receptor is used to demonstrate a successful strategy. The soluble domain of the basic FGF receptor (Lee et al., Science, 245:57 (1989)) is used as the target. A candidate mixture of polypeptide ligands is used as in Example 3. The partitioning of ribosome complexes is obtained with matrix bound extracellular domain of the FGF receptor. The cycles of SPERT are altered to include an elution step from the matrix with high

concentrations of HSV; during this elution step the ribosome complexes that exit the column are discarded, while those ribosome complexes that remain on the column are further eluted with high concentrations of FGF itself. Those ribosome complexes that are not displaced by HSV but are displaced by FGF contain nascent polypeptides that are candidates ligands with the desired

specificity. Such polypeptides bind FGF receptors in a way that inhibits HSV binding but does not interfere with FGF binding. Several cycles of SPERT are used to find the most avidly bound polypeptide that is eluted with FGF but not with HSV. Candidate polypeptides are assayed for their negative impact on HSV infection and their

inability to prevent FGF-mediated cell growth. The most useful polypeptide ligands in this example are neither antagonists nor agonists of the FGF receptor at concentrations that diminish HSV infection. Novel polypeptides meeting these criteria are made using the process as described. A polypeptide meeting the criteria having less than 50% amino acid homology with FGF is isolated. In addition, a polypeptide meeting the criteria having less than 30% homology with FGF is

isolated.

Example 8. Polypeptide ligands that enter

cells: The glucocorticoid receptor and trojan horse ligands. The glucocorticoid receptor protein binds steroid hormone, after which the receptor protein is internalized from the membrane so that the receptor can make its way into the cell nucleus. The receptor has a DNA binding domain (DBD) that interacts in the nucleus with target DNA sequences. Polypeptide ligands of the

invention, agonists of the glucocorticoid

receptor, are internalized along with the

receptor, and thus directed sequentially to the cytoplasm and then to the nucleus. Depending on the dissociation rate constant for specific polypeptide ligands, these ligands largely reside after uptake in either the cytoplasm or the nucleus. Using the randomized starting material of Example 3, SPERT is directed toward the glucocorticoid receptor, either with indirect immunoprecipitation of affinity chromatography using bound receptor. As in prior example, SPERT protocols are manipulated so that polypeptides are found that compete directly for the glucocorticoid binding domain but that have much lower affinity than that observed for steroid hormones. As the polypeptide ligands evolve, screening of potential ligands is performed on individual candidates; thus resistance to proteolysis of the polypeptide ligand is tested using whole cell entry prior to the protease challenge, and testing both cells with and without an abundance of the

glucocorticoid receptor. Polypeptide ligands that enter cells are localized in the cytoplasm or nucleus by means available to those skilled in the art. Those polypeptide ligands that enter cells with proper localization are fused to other polypeptide ligands to provide cell entry for molecules with other useful activities.

Example 9. Polypeptide ligands toward nucleic acids: Inhibitors of transcription.

Cancer cells can result from the over-expression of a transcriptional activator protein that functions to enhance transcription and subsequent expression of sets of genes that push the cell toward inappropriate and uncontrolled growth. Thus, mutations that elevate the activity of a transcriptional enhancer may cause cancer through enhancement of the expression of a set of genes relevant for growth control. Such tumors are treatable with polypeptide ligands that reset the appropriate level of expression or activity of the transcriptional enhancer. While it is likely that polypeptide ligands may be aimed at the enhancer protein directly, thus inhibiting the activity and resetting a proper growth rate, in the present example a polypeptide ligand is aimed at the production rate of the transcriptional enhancer.

The polypeptide ligand of interest binds to the genome of the cancer cell at a location that competes for transcription of the gene that encodes the transcriptional activator protein, and hence expression of that protein. That is, in classical genetic language, the polypeptide ligand is a specific transcriptional repressor.

The starting materials of Example 3 are used to generate a mixed pool of candidate

polypeptides. A specific sequence of double-stranded DNA is prepared by chemical means and covalently attached to an insoluble column matrix. The column matrix is chosen such that ribosome complexes in general are able to flow through the column containing bound DNA. Ribosome complexes containing nascent polypeptide ligands that interact with double-stranded DNA (either with sequence specificity or not) are retarded on the column, recovered, and placed into the SPERT protocol of mRNA-amplification, transcription, and a second cycle. In order to eliminate polypeptide ligands with affinity for all double-stranded DNA (that is, without adequate sequence specificity for the intended use), the ribosome complexes are mixed with random soluble double-stranded DNA sequences prior to the column partitioning step. The soluble DNA concentration is adjusted to give about tenfold more non-specific DNA during the partitioning step than is the abundance of specific DNA sequences attached to the column. In this manner polypeptide ligands that are

indifferent to DNA sequence emerge from the column along with ribosome complexes containing

polypeptide ligands that are unable to bind DNA at all.

Polypeptide ligands aimed at a specific DNA sequence are characterized further.

Randomized DNA sequences are used to establish which nucleotide pairs in the covalently attached DNA are required for avid binding of the

polypeptide (using the SELEX protocol described in U.S. Patent Serial No. 07/536,428). A second SPERT is directed toward the contiguous DNA base pairs that are not bound by the first isolated polypeptide ligand, and the genes for the first and second polypeptide ligands are combined to yield a polypeptide ligand fusion (in either order, and containing a flexible peptide linker) to provide a polypeptide ligand with higher specificity and avidity than is available from either polypeptide ligand by itself. This

improvement in specificity and avidity is an example of walking, although in this case the

"steps" are made independently and the polypeptide ligands joined post-identification.

The sequence of double-stranded DNA chosen in this example must overlap a transcriptional initiation signal. The ras oncogene transcriptional initiation region is chosen first.

Claims

WE CLAIM :

1. A method for making a polypeptide ligand of a target molecule comprising:

a) synthesizing a translatable mRNA mixture comprising a ribosome binding site, translation

initiation codon and a randomized sequence coding region;

b) synthesizing a mixture of ribosome complexes, each member thereof comprising a ribosome, a nascent polypeptide and a translated mRNA, said mRNA having a randomized coding region and said nascent polypeptide being the translation product of said mRNA;

c) partitioning the ribosome complexes with respect to binding of the ribosome complexes to a desired target molecule, thereby separating the ribosome complexes into

ribosome complex-target pairs and unbound complexes, the ribosome complex-target pairs having mRNA enriched for sequences encoding target-binding polypeptides;

d) amplifying the mRNA of partitioned ribosome complex-target pairs to yield a translatable mRNA mixture comprising a ribosome binding site, an initiation codon and a coding region enriched for sequences encoding target-binding polypeptides;

e) repeating steps b) through d) using the mRNA enriched for sequences encoding target-binding

polypeptides of each successive repeat as many times as desired to yield a desired level of target binding by a polypeptide encoded by the mRNA enriched for sequences encoding the polypeptide; and f) synthesizing a polypeptide encoded by the enriched mRNA of step e), thereby making a polypeptide ligand of a target molecule.

2. The method of claim 1 wherein the target molecule is a protein.

3. The method of claim 2 wherein the

protein is an enzyme.

4. The method of claim 2 wherein the

protein is an antibody.

5. The method of claim 2 wherein the

protein is a receptor.

6. The method of claim 2 wherein the

protein is a nucleic acid binding protein.

7. The method of claim 2 wherein the

protein is a toxin.

8. The method of claim 2 wherein the protein is a glycoprotein.

The method of claim 2 wherein the protein is an antigen.

10. The method of claim 1 wherein the

polypeptide is an inhibitor of function of the target molecule.

11. The method of claim 1 wherein the target molecule is a cell membrane component.

12. The method of claim 1 wherein the target molecule is a virus component.

13. The method of claim 1 wherein the target molecule is a carbohydrate.

14. The method of claim 1 wherein the target molecule is a polysaccharide.

15. The method of claim 1 wherein the target molecule is a lipid.

16, The method of claim 1 wherein the target molecule is a glycolipid.

17. The method of claim 1 wherein the target molecule is a toxin.

18. The method of claim 1 wherein the target molecule is a drug.

19. The method of claim 1 wherein the target molecule is a controlled substance.

20. The method of claim 1 wherein the target molecule is a metabolite.

21. The method of claim 1 wherein the target molecule is a cofactor.

22. The method of claim 1 wherein the target molecule is a nucleic acid.

23. The method of claim 1 wherein the target molecule is a hormone.

24. The method of claim 1 wherein the target molecule is a receptor ligand.

25. The method of claim 1 wherein the target molecule is a transition state analog.

26. The method of claim 1 wherein the

translatable mRNA mixture is synthesized by in vitro transcription of a cDNA mixture comprising additionally a transcription promoter sequence near the 5' end of the cDNA.

27. The method of claim 1 wherein the

translation initiation codon is situated within 5-12 bases from the ribosome binding site in the 3 ' direction on the mRNA.

28. The method of claim 1 wherein the ribosome complexes are synthesized in an in vitro translation system lacking release factor.

29. The method of claim 1 wherein the

partitioning is carried out by column chromatography.

30. The method of claim 1 wherein the

partitioning is carried out by binding to target molecules attached to a solid phase matrix.

31. The method of claim 1 wherein the

partitioning is carried out by immunoprecipitation.

32. The method of claim 1 wherein the

partitioning is carried out by indirect immunoprecipitation.

33. The method of claim 1 wherein the mRNA is amplified in step d) by polymerase chain reaction.

34. The method of claim 1 wherein the

process of amplifying in step d)

includes introducing mutations during amplification.

35. The method of claim 1 comprising

additionally, after step e) the step of cloning cDNA of an mRNA selected by the preceding steps.

36. The method of claim 1 wherein the

partitioning step is carried out after post-translationally modifying the nascent polypeptide.

37. The method of claim 1 wherein the

partitioning step is carried out in the presence of a configurational modifier.

38. The method of claim 1 wherein step f) is carried out by chemical synthesis of the polypeptide ligand.

39. The method of claim 35 wherein step f) is carried out by expressing the cloned cDNA in a host cell.

40. The method of claim 1 wherein the mRNA additionally comprises a sequence encoding a C-terminal trailer.

41. The method of claim 1 wherein the mRNA additionally comprises a sequence encoding a segment of polypeptide that functions to bind a bridging molecule and step c) further comprises binding target molecules to a solid phase matrix and binding to the target molecules an anchor molecule covalently bound to the bridging molecule, the anchor molecule being capable of specifically binding the target molecules whereby ribosome complexes bind to the bridging molecule anchored to the target molecules.

42. The method of claim 1 comprising the

additional steps of g) synthesizing a second translatable mRNA mixture

comprising the mRNA selected by steps a) - e) and a second randomized sequence coding region, and h) repeating steps b) - e) using the second translatable mRNA mixture to yield a desired level of target binding by a polypeptide encoded by the second mRNA enriched for sequences encoding the polypeptide.

43. A polypeptide ligand of a target

molecule, said ligand made by the method of claim 1.

44. A polypeptide ligand of a protein, said ligand made by the method of claim 2.

45. A polypeptide ligand of an enzyme, said ligand made by the method of claim 3.

46. A polypeptide ligand of an antibody,

said ligand made by the method of claim 4.

47. A polypeptide ligand of a receptor, said ligand made by the method of claim 5.

48. A polypeptide ligand of a nucleic acid binding protein, said ligand made by the method of claim 6.

49. A polypeptide ligand of a toxin, said ligand made by the method of claim 7.

50. A polypeptide ligand of a glycoprotein, said ligand made by the method of claim 8.

51. A polypeptide ligand of an antigen, said ligand made by the method of claim 9.

52. A polypeptide ligand of a cell membrane component, said ligand made by the method of claim 11.

53. A polypeptide ligand of a virus

component, said ligand made by the method of claim 12.

54. A polypeptide ligand of a carbohydrate, said ligand made by the method of claim 13.

55. A polypeptide ligand of a

polysaccharide, said ligand made by the method of claim 14.

56. A polypeptide ligand of a lipid, said ligand made by the method of claim 15.

57. A polypeptide ligand of a glycolipid, said ligand made by the method of claim 16.

58, A polypeptide ligand of a toxin, said ligand made by the method of claim 17.

59. A polypeptide ligand of a drug, said ligand made by the method of claim 18

60. A polypeptide ligand of a controlled substance, said ligand made by the method of claim 19.

61, A polypeptide ligand of a metabolite, said ligand made by the method of claim 20.

62. A polypeptide ligand of cofactor, said ligand made by the method of claim 21.

63. A polypeptide ligand of a nucleic acid, said ligand made by the method of claim 22.

64 A polypeptide ligand of a hormone, said ligand made by the method of claim 23.

65. A polypeptide ligand of a receptor

ligand, said ligand made by the method of claim 24.

66, A polypeptide ligand of a transition state analog, said ligand made by the method of claim 25.

67. The method for selecting a polypeptide ligand of a desired target molecule from a polypeptide mixture comprising:

a) synthesizing a polypeptide mixture each member thereof having attached thereto amplifying means for separately amplifying the

individual polypeptide to which it is attached;

b) partitioning the polypeptide

mixture with respect to binding the target molecule, thereby separating the mixture into polypeptide-target pairs and unbound polypeptides; c) amplifying the polypeptides of

polypeptide-target pairs using said amplifying means; and d) repeating the partitioning and

amplifying steps to select a polypeptide ligand of a desired target molecule.

68. The method of claim 67 wherein the

polypeptide mixture comprises polypeptides having a segment of

randomized amino acid sequence.

69. The method of claim 68 wherein the

segment of randomized amino acid sequence is from 4 to 50 amino acids in length.

70. The method of claim 67 wherein the

amplifying means comprises an mRNA mixture, each member thereof encoding a polypeptide of the polypeptide mixture and being attached to the polypeptide it encodes as part of a ribosome complex.

71. The method of claim 67 wherein the step of amplifying the polypeptides comprises the additional step of amplifying the mRNA mixture.

72 The method of claim 71 wherein the mRNA mixture is amplified by reverse transcription and a polymerase chain reaction.

73. A polypeptide ligand of a target

molecule, said polypeptide being

selected by the method of claim 64 and said target molecule being selected from the group consisting of a protein, an enzyme, a fusion protein, an antibody, a receptor, a receptor ligand, a nucleic acid, a nucleic acid binding protein, a glycoprotein, a toxin, an antigen, a cell membrane component, a virus, a virus component, a carbohydrate, a polysaccharide, a lipid, a glycolipid, a drug, a controlled substance, a hormone, a transition state analog, a metabolite or a cofactor.

74 A method for selecting a nucleic acid comprising a sequence encoding a polypeptide ligand of a target molecule comprising the steps of: a) synthesizing a translatable mRNA mixture comprising a ribosome binding site, translation

initiation codon and a randomized sequence coding region;

c) partitioning the ribosome complexes with respect to binding of the ribosome complex to a desired target molecule, thereby separating the ribosome complexes into

d) amplifying the mRNA of partitioned ribosome complex-target pairs to yield a translatable mRNA mixture comprising a ribosome binding site, an initiation codon and a coding region enriched for sequences encoding target-binding

polypeptides;

e) repeating steps b) through d) using the mRNA enriched for sequences encoding target-binding polypeptides of each successive repeat as many times as desired to yield as desired level of target binding by a polypeptide encoded by the mRNA enriched for sequences encoding the polypeptide; and f) cloning a cDNA of an mRNA of step e) , thereby selecting a nucleic acid comprising a sequence encoding a polypeptide ligand of a target molecule.

75. A polypeptide ligand of a target

molecule, said polypeptide comprising a non-naturally-occurring sequence of amino acids, and being capable of binding a target molecule selected from the group consisting of a protein, an enzyme, a fusion protein, an antibody, a receptor, a nucleic acid, a nucleic acid binding protein, a glycoprotein, a toxin, an antigen, a cell membrane component, a virus, a virus component, a carbohydrate, a polysaccharide, a lipid, a glycolipid, a drug, a controlled substance, a hormone, a transition state analog, a metabolite or a cofactor.

76. A polypeptide having a minimum of about

44 amino acids and a maximum of about

100 amino acids, comprising of non- naturally-occurring binding segment of length ranging from about 4 to about 50 amino acids and a C-terminal trailer segment of about 40 amino acids, the binding segment having the property of binding to a target molecule.

77. The polypeptide of claim 76 comprising additionally a N-terminal leader segment.

78. The polypeptide of claim 76 wherein the binding segment comprises from about 10 to about 30 amino acids.

79. The polypeptide of claim 76 wherein

binding to the target molecule depends on the simultaneous presence of a configurational modifier.

80. The polypeptide of claim 76 wherein

binding to the target molecule depends on the presence of a disulfide bond in the polypeptide.

81. A polypeptide comprising a binding

segment of about 15-50 amino acids and having less than 50% amino acid homology with a naturally occurring amino acid sequence of the same length, the

polypeptide having the property of binding to a desired target molecule.

82. The polypeptide of claim 81 having less than 30% amino acid homology with a naturally occurring amino acid sequence of the same length.

83. The polypeptide of claim 81 further comprising a naturally-occurring sequence.

84. A method of modifying the function of a naturally-occurring protein comprising replacing a segment of said protein with a polypeptide made by the method of claim 1.

85. A protein modified by the method of

claim 84 to contain a polypeptide having less than 50% homology with a naturally- occurring amino acid sequence of the same length.

86. A protein modified by the method of

claim 84 to contain a polypeptide having less than 30% homology with a naturally- occurring amino acid sequence of the same length.