WO1993002178A1 - Apparatus and methods for electroporation and electrofusion - Google Patents
Apparatus and methods for electroporation and electrofusion Download PDFInfo
- Publication number
- WO1993002178A1 WO1993002178A1 PCT/US1992/005855 US9205855W WO9302178A1 WO 1993002178 A1 WO1993002178 A1 WO 1993002178A1 US 9205855 W US9205855 W US 9205855W WO 9302178 A1 WO9302178 A1 WO 9302178A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- type
- container
- pores
- trapped
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
Definitions
- Electric-field induced fusion has shown promising results.
- electrofusion different types of cells are placed in close contact by applying non-uniform alternating current electric fields to a solution of those cells.
- the electric fields cause dielectrophoresis, which in turn causes cells to move to a region of highest field intensity and organize into formations of variable length.
- Another object of this invention is to provide methods and apparatus for electroporation and electrofusion which minimize damage caused to the cells.
- Figure 4 shows an insulating film with two kinds of cells trapped within a pore
- FIG. 1 is a diagram of a preferred apparatus according to the present invention.
- a container 10 includes an insulating film 20 which is placed across container 10 at about container 10's midpoint to divide container 10 into two portions 35 and 36.
- the exact location of insulated film 20 in container 10 is not critical, however, nor is its shape, as explained below.
- Preferably insulating film is extremely thin, on the order of 10-15 microns.
- Such filters are commercially available, such as the Nuclepore filter manufactured by Costar Corporation of Cambridge, Massachusetts.
- electrofusion can occur by introducing a second type of cell or cell nuclei into portion 36 of container 10 ( Figure 1) , and causing the second cells to move towards the second side 22 of film 20 and contact ones of the trapped first type of cells in pores 25.
- the size of pores 25 in film 20 should be selected so that the proper amount of secondary material, or a single second cell or cell nucleus, can enter a pore in film 20 and be properly oriented adjacent to trapped cell type 1.
- Figure 4 shows insulating film 20 with a portion of a first type of cell 60 being trapped inside pore 28, and a second type of cell 70 also being trapped inside pore 28 adjacent to cell 60.
- One advantage of this invention is that the heating caused by the fields is minimal. If load generator 110 generates 10 volts, the current will be only 10 volts/400 ohms or 0.025A. The heating due to that current, I 2 R, equals (0.025A )2 (400 ohms) or 0.25 watts. This figure, however, is the total power dissipated by the entire circuit. The power dissipation across the filter of the cells is only (0.25A) 2 (150 ohms) or 0.094 watts. Such heating can be kept from harming the cells or material by the heat capacity of the solution itself or, if necessary, by either water jackets or outside cooling.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002113907A CA2113907C (en) | 1991-07-22 | 1992-07-21 | Apparatus and methods for electroporation amd electrofusion |
EP92916662A EP0596003B1 (en) | 1991-07-22 | 1992-07-21 | Apparatus and methods for electroporation and electrofusion |
DE69228309T DE69228309T2 (en) | 1991-07-22 | 1992-07-21 | DEVICE AND METHODS FOR ELECTROPORATION AND ELECTROFUSION |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/733,854 US5173158A (en) | 1991-07-22 | 1991-07-22 | Apparatus and methods for electroporation and electrofusion |
US733,854 | 1991-07-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1993002178A1 true WO1993002178A1 (en) | 1993-02-04 |
Family
ID=24949376
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1992/005855 WO1993002178A1 (en) | 1991-07-22 | 1992-07-21 | Apparatus and methods for electroporation and electrofusion |
Country Status (7)
Country | Link |
---|---|
US (2) | US5173158A (en) |
EP (1) | EP0596003B1 (en) |
AT (1) | ATE176278T1 (en) |
AU (1) | AU2378092A (en) |
CA (1) | CA2113907C (en) |
DE (1) | DE69228309T2 (en) |
WO (1) | WO1993002178A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7358077B2 (en) | 2002-01-21 | 2008-04-15 | Eppendorf Ag | Method and device for electroporation of biological cells |
Families Citing this family (129)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6010613A (en) * | 1995-12-08 | 2000-01-04 | Cyto Pulse Sciences, Inc. | Method of treating materials with pulsed electrical fields |
WO1998022819A1 (en) | 1996-11-16 | 1998-05-28 | Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen Stiftung Bürgerlichen Rechts | Array of microelements, method of contacting cells in a liquid environment and method for the production of an array of microelements |
US6043066A (en) * | 1997-09-04 | 2000-03-28 | Mangano; Joseph A. | Cell separation using electric fields |
AU733053C (en) | 1998-02-24 | 2001-11-29 | Boston Scientific Limited | High flow rate dialysis catheters and related methods |
DE19841337C1 (en) | 1998-05-27 | 1999-09-23 | Micronas Intermetall Gmbh | Intracellular manipulation of biological cell contents, assisting injection or removal of substances or cell components |
DE19827957C2 (en) * | 1998-05-27 | 2000-06-29 | Micronas Intermetall Gmbh | Method and device for measuring a state variable |
GB9812519D0 (en) * | 1998-06-10 | 1998-08-05 | Immunoporation Ltd | Treating cells |
WO1999064581A1 (en) | 1998-06-10 | 1999-12-16 | University Of South Florida | Electrofusion chamber |
GB9812783D0 (en) | 1998-06-12 | 1998-08-12 | Cenes Ltd | High throuoghput screen |
IL140918A0 (en) | 1998-07-27 | 2002-02-10 | Genentech Inc | Improved transformation efficiency in phage display through modification of a coat protein |
GB9908681D0 (en) | 1999-04-16 | 1999-06-09 | Central Research Lab Ltd | Apparatus for, and method of, introducing a substance into an object |
US6300108B1 (en) * | 1999-07-21 | 2001-10-09 | The Regents Of The University Of California | Controlled electroporation and mass transfer across cell membranes |
US6927049B2 (en) * | 1999-07-21 | 2005-08-09 | The Regents Of The University Of California | Cell viability detection using electrical measurements |
US6387671B1 (en) | 1999-07-21 | 2002-05-14 | The Regents Of The University Of California | Electrical impedance tomography to control electroporation |
US6403348B1 (en) | 1999-07-21 | 2002-06-11 | The Regents Of The University Of California | Controlled electroporation and mass transfer across cell membranes |
US7053063B2 (en) * | 1999-07-21 | 2006-05-30 | The Regents Of The University Of California | Controlled electroporation and mass transfer across cell membranes in tissue |
JP4740503B2 (en) * | 2000-01-27 | 2011-08-03 | 株式会社モレニアムラボラトリーズ | Molecule introduction apparatus and molecule introduction method |
US6795728B2 (en) | 2001-08-17 | 2004-09-21 | Minnesota Medical Physics, Llc | Apparatus and method for reducing subcutaneous fat deposits by electroporation |
US8251986B2 (en) | 2000-08-17 | 2012-08-28 | Angiodynamics, Inc. | Method of destroying tissue cells by eletroporation |
US6892099B2 (en) * | 2001-02-08 | 2005-05-10 | Minnesota Medical Physics, Llc | Apparatus and method for reducing subcutaneous fat deposits, virtual face lift and body sculpturing by electroporation |
US6697670B2 (en) | 2001-08-17 | 2004-02-24 | Minnesota Medical Physics, Llc | Apparatus and method for reducing subcutaneous fat deposits by electroporation with improved comfort of patients |
USRE42016E1 (en) | 2001-08-13 | 2010-12-28 | Angiodynamics, Inc. | Apparatus and method for the treatment of benign prostatic hyperplasia |
US6994706B2 (en) | 2001-08-13 | 2006-02-07 | Minnesota Medical Physics, Llc | Apparatus and method for treatment of benign prostatic hyperplasia |
US7130697B2 (en) * | 2002-08-13 | 2006-10-31 | Minnesota Medical Physics Llc | Apparatus and method for the treatment of benign prostatic hyperplasia |
WO2003020915A2 (en) * | 2001-08-31 | 2003-03-13 | Cyto Pulse Sciences, Inc. | Non-linear amplitude dielectrophoresis waveform for cell fusion |
WO2003050232A1 (en) * | 2001-12-06 | 2003-06-19 | Bio-Rad Laboratories, Inc. | Electroporation cuvette insert for facilitating membrane-based fusion |
ATE364077T1 (en) * | 2002-02-22 | 2007-06-15 | Idm Immuno Designed Molecules | METHOD FOR ELECTROFUSION OF CELLS |
JP4273231B2 (en) * | 2002-07-16 | 2009-06-03 | 独立行政法人農業生物資源研究所 | Electroporation method including the use of reduced pressure / pressurization |
ES2375724T3 (en) | 2002-09-27 | 2012-03-05 | The General Hospital Corporation | MICROFLUDE DEVICE FOR SEPERATION OF CELLS AND ITS USES. |
EP1565555A4 (en) * | 2002-09-30 | 2008-07-09 | Maxcyte Inc | Apparatus and method for streaming electroporation |
JP5138229B2 (en) | 2003-12-24 | 2013-02-06 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Tissue ablation by irreversible electroporation |
US8298222B2 (en) * | 2003-12-24 | 2012-10-30 | The Regents Of The University Of California | Electroporation to deliver chemotherapeutics and enhance tumor regression |
JP4443278B2 (en) * | 2004-03-26 | 2010-03-31 | テルモ株式会社 | Catheter with expansion body |
US20060292554A1 (en) * | 2004-05-18 | 2006-12-28 | Genentech, Inc. | Major coat protein variants for C-terminal and bi-terminal display |
JPWO2006098430A1 (en) * | 2005-03-17 | 2008-08-28 | 富士フイルム株式会社 | Intracellular substance introduction device, cell clamping device and flow path forming method |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
US20060264752A1 (en) * | 2005-04-27 | 2006-11-23 | The Regents Of The University Of California | Electroporation controlled with real time imaging |
US20060281168A1 (en) | 2005-06-13 | 2006-12-14 | Tosoh Corporation | Cell fusion chamber, cell fusion device, and method for cell fusion using the same |
US20060293725A1 (en) * | 2005-06-24 | 2006-12-28 | Boris Rubinsky | Methods and systems for treating fatty tissue sites using electroporation |
US8114070B2 (en) * | 2005-06-24 | 2012-02-14 | Angiodynamics, Inc. | Methods and systems for treating BPH using electroporation |
US20060293730A1 (en) | 2005-06-24 | 2006-12-28 | Boris Rubinsky | Methods and systems for treating restenosis sites using electroporation |
US20060293731A1 (en) * | 2005-06-24 | 2006-12-28 | Boris Rubinsky | Methods and systems for treating tumors using electroporation |
US8214221B2 (en) * | 2005-06-30 | 2012-07-03 | Lg Electronics Inc. | Method and apparatus for decoding an audio signal and identifying information included in the audio signal |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US20070105206A1 (en) * | 2005-10-19 | 2007-05-10 | Chang Lu | Fluidic device |
US20070156135A1 (en) * | 2006-01-03 | 2007-07-05 | Boris Rubinsky | System and methods for treating atrial fibrillation using electroporation |
EP2076313A4 (en) | 2006-10-16 | 2012-07-25 | Univ California | Gels with predetermined conductivity used in irreversible electroporation of tissue |
US20080132884A1 (en) * | 2006-12-01 | 2008-06-05 | Boris Rubinsky | Systems for treating tissue sites using electroporation |
EP3190178A1 (en) | 2007-02-23 | 2017-07-12 | Astellas Institute for Regenerative Medicine | Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells |
US20100004623A1 (en) * | 2008-03-27 | 2010-01-07 | Angiodynamics, Inc. | Method for Treatment of Complications Associated with Arteriovenous Grafts and Fistulas Using Electroporation |
US20090247933A1 (en) | 2008-03-27 | 2009-10-01 | The Regents Of The University Of California; Angiodynamics, Inc. | Balloon catheter method for reducing restenosis via irreversible electroporation |
US8992517B2 (en) | 2008-04-29 | 2015-03-31 | Virginia Tech Intellectual Properties Inc. | Irreversible electroporation to treat aberrant cell masses |
US9283051B2 (en) | 2008-04-29 | 2016-03-15 | Virginia Tech Intellectual Properties, Inc. | System and method for estimating a treatment volume for administering electrical-energy based therapies |
US10117707B2 (en) | 2008-04-29 | 2018-11-06 | Virginia Tech Intellectual Properties, Inc. | System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies |
US10238447B2 (en) | 2008-04-29 | 2019-03-26 | Virginia Tech Intellectual Properties, Inc. | System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress |
US11272979B2 (en) | 2008-04-29 | 2022-03-15 | Virginia Tech Intellectual Properties, Inc. | System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies |
US9867652B2 (en) | 2008-04-29 | 2018-01-16 | Virginia Tech Intellectual Properties, Inc. | Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds |
US9198733B2 (en) | 2008-04-29 | 2015-12-01 | Virginia Tech Intellectual Properties, Inc. | Treatment planning for electroporation-based therapies |
US10702326B2 (en) | 2011-07-15 | 2020-07-07 | Virginia Tech Intellectual Properties, Inc. | Device and method for electroporation based treatment of stenosis of a tubular body part |
US10245098B2 (en) | 2008-04-29 | 2019-04-02 | Virginia Tech Intellectual Properties, Inc. | Acute blood-brain barrier disruption using electrical energy based therapy |
US10448989B2 (en) | 2009-04-09 | 2019-10-22 | Virginia Tech Intellectual Properties, Inc. | High-frequency electroporation for cancer therapy |
US11254926B2 (en) | 2008-04-29 | 2022-02-22 | Virginia Tech Intellectual Properties, Inc. | Devices and methods for high frequency electroporation |
US10272178B2 (en) | 2008-04-29 | 2019-04-30 | Virginia Tech Intellectual Properties Inc. | Methods for blood-brain barrier disruption using electrical energy |
AU2009243079A1 (en) | 2008-04-29 | 2009-11-05 | Virginia Tech Intellectual Properties, Inc. | Irreversible electroporation to create tissue scaffolds |
WO2009137800A2 (en) | 2008-05-09 | 2009-11-12 | Angiodynamics, Inc. | Electroporation device and method |
WO2009155526A2 (en) | 2008-06-20 | 2009-12-23 | Angiodynamics, Inc. | Device and method for the ablation of fibrin sheath formation on a venous catheter |
WO2010008834A2 (en) | 2008-06-23 | 2010-01-21 | Angiodynamics, Inc. | Treatment devices and methods |
US20100152725A1 (en) * | 2008-12-12 | 2010-06-17 | Angiodynamics, Inc. | Method and system for tissue treatment utilizing irreversible electroporation and thermal track coagulation |
WO2010085765A2 (en) | 2009-01-23 | 2010-07-29 | Moshe Meir H | Therapeutic energy delivery device with rotational mechanism |
WO2010093692A2 (en) | 2009-02-10 | 2010-08-19 | Hobbs Eamonn P | Irreversible electroporation and tissue regeneration |
US11382681B2 (en) | 2009-04-09 | 2022-07-12 | Virginia Tech Intellectual Properties, Inc. | Device and methods for delivery of high frequency electrical pulses for non-thermal ablation |
WO2010118387A1 (en) | 2009-04-09 | 2010-10-14 | Virginia Tech Intellectual Properties, Inc. | Integration of very short electric pulses for minimally to noninvasive electroporation |
US11638603B2 (en) | 2009-04-09 | 2023-05-02 | Virginia Tech Intellectual Properties, Inc. | Selective modulation of intracellular effects of cells using pulsed electric fields |
USD630321S1 (en) | 2009-05-08 | 2011-01-04 | Angio Dynamics, Inc. | Probe handle |
US8903488B2 (en) | 2009-05-28 | 2014-12-02 | Angiodynamics, Inc. | System and method for synchronizing energy delivery to the cardiac rhythm |
US9895189B2 (en) | 2009-06-19 | 2018-02-20 | Angiodynamics, Inc. | Methods of sterilization and treating infection using irreversible electroporation |
US20110118732A1 (en) | 2009-11-19 | 2011-05-19 | The Regents Of The University Of California | Controlled irreversible electroporation |
US9816086B2 (en) * | 2010-07-06 | 2017-11-14 | The Ohio State University | Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation |
US9700368B2 (en) | 2010-10-13 | 2017-07-11 | Angiodynamics, Inc. | System and method for electrically ablating tissue of a patient |
US9382510B2 (en) | 2011-08-25 | 2016-07-05 | Jian Chen | Methods and devices for electroporation |
US9078665B2 (en) | 2011-09-28 | 2015-07-14 | Angiodynamics, Inc. | Multiple treatment zone ablation probe |
US9414881B2 (en) | 2012-02-08 | 2016-08-16 | Angiodynamics, Inc. | System and method for increasing a target zone for electrical ablation |
US9888956B2 (en) | 2013-01-22 | 2018-02-13 | Angiodynamics, Inc. | Integrated pump and generator device and method of use |
US10166321B2 (en) | 2014-01-09 | 2019-01-01 | Angiodynamics, Inc. | High-flow port and infusion needle systems |
US10471254B2 (en) | 2014-05-12 | 2019-11-12 | Virginia Tech Intellectual Properties, Inc. | Selective modulation of intracellular effects of cells using pulsed electric fields |
KR102473092B1 (en) | 2014-09-15 | 2022-12-01 | 칠드런'즈 메디컬 센터 코포레이션 | Methods and compositions to increase somatic cell nuclear transfer (scnt) efficiency by removing histone h3-lysine trimethylation |
WO2016100325A1 (en) | 2014-12-15 | 2016-06-23 | Virginia Tech Intellectual Properties, Inc. | Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment |
US10660691B2 (en) | 2015-10-07 | 2020-05-26 | Angiodynamics, Inc. | Multiple use subassembly with integrated fluid delivery system for use with single or dual-lumen peristaltic tubing |
US11390840B2 (en) * | 2016-09-30 | 2022-07-19 | University Of Florida Research Foundation, Inc. | Systems and methods including porous membrane for low-voltage continuous cell electroporation |
US10905492B2 (en) | 2016-11-17 | 2021-02-02 | Angiodynamics, Inc. | Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode |
US11043823B2 (en) * | 2017-04-06 | 2021-06-22 | Tesla, Inc. | System and method for facilitating conditioning and testing of rechargeable battery cells |
JP2021503281A (en) | 2017-11-17 | 2021-02-12 | アイオバンス バイオセラピューティクス,インコーポレイテッド | TIL expansion culture from fine needle suction and small biopsy |
EP3714041A1 (en) | 2017-11-22 | 2020-09-30 | Iovance Biotherapeutics, Inc. | Expansion of peripheral blood lymphocytes (pbls) from peripheral blood |
US11607537B2 (en) | 2017-12-05 | 2023-03-21 | Virginia Tech Intellectual Properties, Inc. | Method for treating neurological disorders, including tumors, with electroporation |
US11925405B2 (en) | 2018-03-13 | 2024-03-12 | Virginia Tech Intellectual Properties, Inc. | Treatment planning system for immunotherapy enhancement via non-thermal ablation |
US11311329B2 (en) | 2018-03-13 | 2022-04-26 | Virginia Tech Intellectual Properties, Inc. | Treatment planning for immunotherapy based treatments using non-thermal ablation techniques |
WO2019210131A1 (en) | 2018-04-27 | 2019-10-31 | Iovance Biotherapeutics, Inc. | Closed process for expansion and gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy |
IT201800006914A1 (en) * | 2018-07-04 | 2020-01-04 | ELECTRICAL CHARACTERIZATION DEVICE FOR CELLULAR BUILDINGS | |
JOP20210094A1 (en) | 2018-11-05 | 2023-01-30 | Iovance Biotherapeutics Inc | Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy |
BR112021008549A2 (en) | 2018-11-05 | 2022-01-04 | Iovance Biotherapeutics Inc | Method of treating non-small cell lung cancer with a population of tumor-infiltrating lymphocytes |
MX2021004953A (en) | 2018-11-05 | 2021-08-11 | Iovance Biotherapeutics Inc | Selection of improved tumor reactive t-cells. |
WO2020096927A1 (en) | 2018-11-05 | 2020-05-14 | Iovance Biotherapeutics, Inc. | Expansion of tils utilizing akt pathway inhibitors |
CA3123392A1 (en) | 2018-12-19 | 2020-06-25 | Iovance Biotherapeutics, Inc. | Methods of expanding tumor infiltrating lymphocytes using engineered cytokine receptor pairs and uses thereof |
CN113748202A (en) | 2019-03-01 | 2021-12-03 | 艾欧凡斯生物治疗公司 | Expansion of tumor infiltrating lymphocytes from liquid tumors and therapeutic uses thereof |
WO2020232029A1 (en) | 2019-05-13 | 2020-11-19 | Iovance Biotherapeutics, Inc. | Methods and compositions for selecting tumor infiltrating lymphocytes and uses of the same in immunotherapy |
US11950835B2 (en) | 2019-06-28 | 2024-04-09 | Virginia Tech Intellectual Properties, Inc. | Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy |
WO2021081378A1 (en) | 2019-10-25 | 2021-04-29 | Iovance Biotherapeutics, Inc. | Gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy |
JP2023506734A (en) | 2019-12-11 | 2023-02-20 | アイオバンス バイオセラピューティクス,インコーポレイテッド | Process for the production of tumor-infiltrating lymphocytes (TIL) and methods of using same |
EP4146794A1 (en) | 2020-05-04 | 2023-03-15 | Iovance Biotherapeutics, Inc. | Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy |
WO2022076606A1 (en) | 2020-10-06 | 2022-04-14 | Iovance Biotherapeutics, Inc. | Treatment of nsclc patients with tumor infiltrating lymphocyte therapies |
EP4225330A1 (en) | 2020-10-06 | 2023-08-16 | Iovance Biotherapeutics, Inc. | Treatment of nsclc patients with tumor infiltrating lymphocyte therapies |
WO2022133140A1 (en) | 2020-12-17 | 2022-06-23 | Iovance Biotherapeutics, Inc. | Treatment with tumor infiltrating lymphocyte therapies in combination with ctla-4 and pd-1 inhibitors |
EP4262827A1 (en) | 2020-12-17 | 2023-10-25 | Iovance Biotherapeutics, Inc. | Treatment of cancers with tumor infiltrating lymphocytes |
EP4284919A1 (en) | 2021-01-29 | 2023-12-06 | Iovance Biotherapeutics, Inc. | Methods of making modified tumor infiltrating lymphocytes and their use in adoptive cell therapy |
EP4308691A1 (en) | 2021-03-19 | 2024-01-24 | Iovance Biotherapeutics, Inc. | Methods for tumor infiltrating lymphocyte (til) expansion related to cd39/cd69 selection and gene knockout in tils |
JP2024511414A (en) | 2021-03-23 | 2024-03-13 | アイオバンス バイオセラピューティクス,インコーポレイテッド | CISH gene editing of tumor-infiltrating lymphocytes and its use in immunotherapy |
IL307800A (en) | 2021-04-19 | 2023-12-01 | Iovance Biotherapeutics Inc | Chimeric costimulatory receptors, chemokine receptors, and the use of same in cellular immunotherapies |
CA3219148A1 (en) | 2021-05-17 | 2022-11-24 | Frederick G. Vogt | Pd-1 gene-edited tumor infiltrating lymphocytes and uses of same in immunotherapy |
WO2023004074A2 (en) | 2021-07-22 | 2023-01-26 | Iovance Biotherapeutics, Inc. | Method for cryopreservation of solid tumor fragments |
TW202327631A (en) | 2021-07-28 | 2023-07-16 | 美商艾歐凡斯生物治療公司 | Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with kras inhibitors |
CA3231018A1 (en) | 2021-09-09 | 2023-03-16 | Iovance Biotherapeutics, Inc. | Processes for generating til products using pd-1 talen knockdown |
US20230187042A1 (en) | 2021-10-27 | 2023-06-15 | Iovance Biotherapeutics, Inc. | Systems and methods for coordinating manufacturing of cells for patient-specific immunotherapy |
WO2023086803A1 (en) | 2021-11-10 | 2023-05-19 | Iovance Biotherapeutics, Inc. | Methods of expansion treatment utilizing cd8 tumor infiltrating lymphocytes |
WO2023147488A1 (en) | 2022-01-28 | 2023-08-03 | Iovance Biotherapeutics, Inc. | Cytokine associated tumor infiltrating lymphocytes compositions and methods |
WO2023196877A1 (en) | 2022-04-06 | 2023-10-12 | Iovance Biotherapeutics, Inc. | Treatment of nsclc patients with tumor infiltrating lymphocyte therapies |
WO2023201369A1 (en) | 2022-04-15 | 2023-10-19 | Iovance Biotherapeutics, Inc. | Til expansion processes using specific cytokine combinations and/or akti treatment |
WO2023220608A1 (en) | 2022-05-10 | 2023-11-16 | Iovance Biotherapeutics, Inc. | Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with an il-15r agonist |
WO2024055018A1 (en) | 2022-09-09 | 2024-03-14 | Iovance Biotherapeutics, Inc. | Processes for generating til products using pd-1/tigit talen double knockdown |
WO2024055017A1 (en) | 2022-09-09 | 2024-03-14 | Iovance Biotherapeutics, Inc. | Processes for generating til products using pd-1/tigit talen double knockdown |
Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1985002201A1 (en) * | 1983-11-08 | 1985-05-23 | Scientific Diagnostics, Inc. | System and methods for cell selection |
JPS6384477A (en) * | 1986-09-26 | 1988-04-15 | Shimadzu Corp | Cell-fusion chamber |
JPS6384478A (en) * | 1986-09-26 | 1988-04-15 | Shimadzu Corp | Cell-fusion chamber |
JPS63173574A (en) * | 1987-01-13 | 1988-07-18 | Nok Corp | Electrode for cell fusion |
JPS63181992A (en) * | 1987-01-24 | 1988-07-27 | Advance Co Ltd | Operating device for fine particle |
JPS63202368A (en) * | 1987-02-18 | 1988-08-22 | Hitachi Ltd | Miniature vessel for cell fusion |
JPS63230070A (en) * | 1987-03-18 | 1988-09-26 | Shimadzu Corp | Cell fusion chamber |
JPS63258572A (en) * | 1987-04-17 | 1988-10-26 | Norin Suisan Gijutsu Joho Kyokai | Cell-fusion apparatus |
JPS63269977A (en) * | 1987-04-30 | 1988-11-08 | Hitachi Ltd | Cell fusion apparatus |
JPS6480278A (en) * | 1987-09-21 | 1989-03-27 | Hitachi Ltd | Chamber plate for holding cell |
JPH01112976A (en) * | 1987-10-28 | 1989-05-01 | Hitachi Ltd | Micro-injection device |
JPH01174371A (en) * | 1987-12-29 | 1989-07-10 | Shimadzu Corp | Cell fusing chamber |
JPH01191676A (en) * | 1988-01-25 | 1989-08-01 | Hitachi Ltd | Chamber for cell fusion |
JPH01223348A (en) * | 1988-03-02 | 1989-09-06 | Omron Tateisi Electron Co | Particle immobilizing apparatus |
JPH01247076A (en) * | 1988-03-30 | 1989-10-02 | Shimadzu Corp | Method and apparatus for fusing cell |
JPH022332A (en) * | 1988-04-15 | 1990-01-08 | Hitachi Ltd | Apparatus for cell fusion |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3870617A (en) * | 1971-03-30 | 1975-03-11 | Rhone Poulenc Sa | Apparatus for forced flow electrophoresis |
GB1481480A (en) * | 1974-02-02 | 1977-07-27 | Kernforschungsanlage Juelich | Process and apparatus for increasing the permeability of the membrane of cells of organisms |
DE2656746C2 (en) * | 1976-12-15 | 1986-06-26 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Use of loaded red blood cells |
US4849355A (en) * | 1985-01-08 | 1989-07-18 | Wong Tai Kin | Method of transferring genes into cells |
US4578167A (en) * | 1982-09-28 | 1986-03-25 | Biofusion, Inc. | Cell fusion |
US4441972A (en) * | 1983-04-08 | 1984-04-10 | D.E.P. Systems, Inc. | Apparatus for electrofusion of biological particles |
US4661451A (en) * | 1984-02-06 | 1987-04-28 | Ortho Diagnostic Systems, Inc. | Methods for immobilizing and translocating biological cells |
US4578168A (en) * | 1984-07-27 | 1986-03-25 | Biotronics | Apparatus for fusing live cells with electric fields |
US4663292A (en) * | 1984-12-21 | 1987-05-05 | Wong Daniel T | High-voltage biological macromolecule transfer and cell fusion system |
DE3505147A1 (en) * | 1985-02-15 | 1986-10-23 | GCA Corp., Bedford, Mass. | Process and apparatus for specific fusion of cells |
FR2583769B1 (en) * | 1985-06-19 | 1987-09-18 | Centre Nat Rech Scient | IMPROVED CELL FUSION PROCESS |
KR890003947B1 (en) * | 1985-12-11 | 1989-10-13 | 가부시기가이샤 시마즈세이사구쇼 | Apparatus for cell fusion |
US4906576A (en) * | 1986-05-09 | 1990-03-06 | Electropore, Inc. | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor |
US4849089A (en) * | 1986-05-09 | 1989-07-18 | Electropore, Inc. | Disposable electromanipulation chamber |
US4923814A (en) * | 1986-05-09 | 1990-05-08 | Electropore, Inc. | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor |
JP2662215B2 (en) * | 1986-11-19 | 1997-10-08 | 株式会社日立製作所 | Cell holding device |
US4800163A (en) * | 1986-12-15 | 1989-01-24 | Ntl. Inst. of Agrobiological Resources | Flow chamber and electro-manipulator incorporating same |
US4822470A (en) * | 1987-10-09 | 1989-04-18 | Baylor College Of Medicine | Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses |
JPH01141582A (en) * | 1987-11-27 | 1989-06-02 | Shimadzu Corp | Gene-introducing device |
US4832814A (en) * | 1987-12-28 | 1989-05-23 | E. I. Du Pont De Nemours And Company | Electrofusion cell and method of making the same |
US4910140A (en) * | 1988-04-18 | 1990-03-20 | Bio-Rad Laboratories, Inc. | Electroporation of prokaryotic cells |
-
1991
- 1991-07-22 US US07/733,854 patent/US5173158A/en not_active Expired - Lifetime
-
1992
- 1992-07-16 US US07/913,612 patent/US5283194A/en not_active Expired - Lifetime
- 1992-07-21 DE DE69228309T patent/DE69228309T2/en not_active Expired - Fee Related
- 1992-07-21 WO PCT/US1992/005855 patent/WO1993002178A1/en active Search and Examination
- 1992-07-21 CA CA002113907A patent/CA2113907C/en not_active Expired - Fee Related
- 1992-07-21 AT AT92916662T patent/ATE176278T1/en active
- 1992-07-21 AU AU23780/92A patent/AU2378092A/en not_active Abandoned
- 1992-07-21 EP EP92916662A patent/EP0596003B1/en not_active Expired - Lifetime
Patent Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1985002201A1 (en) * | 1983-11-08 | 1985-05-23 | Scientific Diagnostics, Inc. | System and methods for cell selection |
JPS6384477A (en) * | 1986-09-26 | 1988-04-15 | Shimadzu Corp | Cell-fusion chamber |
JPS6384478A (en) * | 1986-09-26 | 1988-04-15 | Shimadzu Corp | Cell-fusion chamber |
JPS63173574A (en) * | 1987-01-13 | 1988-07-18 | Nok Corp | Electrode for cell fusion |
JPS63181992A (en) * | 1987-01-24 | 1988-07-27 | Advance Co Ltd | Operating device for fine particle |
JPS63202368A (en) * | 1987-02-18 | 1988-08-22 | Hitachi Ltd | Miniature vessel for cell fusion |
JPS63230070A (en) * | 1987-03-18 | 1988-09-26 | Shimadzu Corp | Cell fusion chamber |
JPS63258572A (en) * | 1987-04-17 | 1988-10-26 | Norin Suisan Gijutsu Joho Kyokai | Cell-fusion apparatus |
JPS63269977A (en) * | 1987-04-30 | 1988-11-08 | Hitachi Ltd | Cell fusion apparatus |
JPS6480278A (en) * | 1987-09-21 | 1989-03-27 | Hitachi Ltd | Chamber plate for holding cell |
JPH01112976A (en) * | 1987-10-28 | 1989-05-01 | Hitachi Ltd | Micro-injection device |
JPH01174371A (en) * | 1987-12-29 | 1989-07-10 | Shimadzu Corp | Cell fusing chamber |
JPH01191676A (en) * | 1988-01-25 | 1989-08-01 | Hitachi Ltd | Chamber for cell fusion |
JPH01223348A (en) * | 1988-03-02 | 1989-09-06 | Omron Tateisi Electron Co | Particle immobilizing apparatus |
JPH01247076A (en) * | 1988-03-30 | 1989-10-02 | Shimadzu Corp | Method and apparatus for fusing cell |
JPH022332A (en) * | 1988-04-15 | 1990-01-08 | Hitachi Ltd | Apparatus for cell fusion |
Non-Patent Citations (1)
Title |
---|
ROBERT SCHUMUKLER and ARTHUR A. PILLA, "A transient Impedance Approach to Nonfaradaic Electrochemical Kinetics at Living Cell Membranes", Journal of the Electrochemical Society, Vol. 129, No. 3 (1982) 526-528. * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7358077B2 (en) | 2002-01-21 | 2008-04-15 | Eppendorf Ag | Method and device for electroporation of biological cells |
US8465955B2 (en) | 2002-01-21 | 2013-06-18 | Eppendorf Ag | Method for electroporation of biological cells |
Also Published As
Publication number | Publication date |
---|---|
ATE176278T1 (en) | 1999-02-15 |
CA2113907A1 (en) | 1993-02-04 |
US5283194A (en) | 1994-02-01 |
US5173158A (en) | 1992-12-22 |
EP0596003A1 (en) | 1994-05-11 |
EP0596003B1 (en) | 1999-01-27 |
DE69228309T2 (en) | 1999-09-16 |
AU2378092A (en) | 1993-02-23 |
CA2113907C (en) | 2002-12-24 |
DE69228309D1 (en) | 1999-03-11 |
EP0596003A4 (en) | 1995-12-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0596003B1 (en) | Apparatus and methods for electroporation and electrofusion | |
US4923814A (en) | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor | |
US20190161720A1 (en) | Methods and devices for electroporation | |
US4764473A (en) | Chamber for the treatment of cells in an electrical field | |
EP0925647B1 (en) | Electroporation apparatus employing user-configured pulses | |
US4822470A (en) | Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses | |
US20210324367A1 (en) | Method and device for uniformly treating adherent cells | |
US8465955B2 (en) | Method for electroporation of biological cells | |
Li et al. | Electrofusion between heterogeneous-sized mammalian cells in a pellet: potential applications in drug delivery and hybridoma formation | |
US4959321A (en) | Cell fusion apparatus | |
US6221665B1 (en) | Electrofusion chamber | |
Hofmann | Cells in electric fields: Physical and practical electronic aspects of electro cell fusion and electroporation | |
Tsong | [25] Electric modification of membrane permeability for drug loading into living cells | |
CN217173753U (en) | Ring type transfection device utilizing six-hole plate | |
US11959061B2 (en) | Methods and devices for electroporation | |
Naton et al. | Improved electrofusion of protoplasts of varied fusibility by selective pairing: application of asymmetric breakdown of plasma membranes | |
JPS6054688A (en) | Method for fusing cell | |
WO1987006851A1 (en) | Apparatus and method for vesicle poration, loading and fusion | |
Jaroszeski et al. | Electrofusion chamber | |
Dura et al. | CELL SQUEEZING-BASED SEQUENTIAL CAPTURE, PAIRING AND FUSION OF CELLS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AT AU BB BG BR CA CH CS DE DK ES FI GB HU JP KP KR LK LU MG MN MW NL NO PL RO RU SD SE |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE BF BJ CF CG CI CM GA GN ML MR SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) |
Free format text: PL |
|
LE32 | Later election for international application filed prior to expiration of 19th month from priority date or according to rule 32.2 (b) |
Ref country code: UA |
|
EX32 | Extension under rule 32 effected after completion of technical preparation for international publication |
Ref country code: UA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2113907 Country of ref document: CA Ref document number: 1992916662 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 1992916662 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 1992916662 Country of ref document: EP |
|
DPE2 | Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101) |