WO1995005472A3 - Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants - Google Patents

Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants Download PDF

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Publication number
WO1995005472A3
WO1995005472A3 PCT/US1994/009265 US9409265W WO9505472A3 WO 1995005472 A3 WO1995005472 A3 WO 1995005472A3 US 9409265 W US9409265 W US 9409265W WO 9505472 A3 WO9505472 A3 WO 9505472A3
Authority
WO
WIPO (PCT)
Prior art keywords
plastids
processes
phb
higher plants
polyhydroxyalkanoates
Prior art date
Application number
PCT/US1994/009265
Other languages
French (fr)
Other versions
WO1995005472A2 (en
Inventor
Christopher Roland Somerville
Christiane Nawrath
Yves Poirier
Original Assignee
Univ Michigan State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan State filed Critical Univ Michigan State
Priority to JP7507156A priority Critical patent/JPH09501832A/en
Priority to DE69434377T priority patent/DE69434377T2/en
Priority to AU76330/94A priority patent/AU7633094A/en
Priority to AT94926515T priority patent/ATE295894T1/en
Priority to EP94926515A priority patent/EP0719342B1/en
Publication of WO1995005472A2 publication Critical patent/WO1995005472A2/en
Publication of WO1995005472A3 publication Critical patent/WO1995005472A3/en
Priority to HK98114395A priority patent/HK1013110A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

Abstract

The present invention relates to a process for producing poly-D-(-)-3-hydroxybutyric acid (PHB) and related polyhydroxyalkanoates (PHA) in the plastids of plants. The production of PHB is accomplished by genetically transforming plants with modified genes from microorganisms. The genes encode the enzymes required to synthesize PHB from acetyl-CoA or related metabolites and are fused with additional plant sequences for targeting the enzymes to the plastid.
PCT/US1994/009265 1993-08-17 1994-08-17 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants WO1995005472A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP7507156A JPH09501832A (en) 1993-08-17 1994-08-17 Method for producing polyhydroxybutyrate and related polyhydroxyalkanoates in higher plant plastids
DE69434377T DE69434377T2 (en) 1993-08-17 1994-08-17 PROCESS FOR THE PREPARATION OF POLYHYDROXYBUTYRATE AND RELATED POLYHYDROXYALKANOATES IN THE PLASTID HOEHERER PLANTS
AU76330/94A AU7633094A (en) 1993-08-17 1994-08-17 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
AT94926515T ATE295894T1 (en) 1993-08-17 1994-08-17 METHOD FOR PRODUCING POLYHYDROXYBUTYRATE AND RELATED POLYHYDROXYALKANOATES IN THE PLASTIDS OF HIGHER PLANTS
EP94926515A EP0719342B1 (en) 1993-08-17 1994-08-17 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
HK98114395A HK1013110A1 (en) 1993-08-17 1998-12-21 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10819393A 1993-08-17 1993-08-17
US08/108,193 1993-08-17
US08/254,357 1994-06-06
US08/254,357 US5610041A (en) 1991-07-19 1994-06-06 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

Publications (2)

Publication Number Publication Date
WO1995005472A2 WO1995005472A2 (en) 1995-02-23
WO1995005472A3 true WO1995005472A3 (en) 1995-03-30

Family

ID=26805624

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/009265 WO1995005472A2 (en) 1993-08-17 1994-08-17 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

Country Status (9)

Country Link
US (1) US5610041A (en)
EP (1) EP0719342B1 (en)
JP (1) JPH09501832A (en)
AT (1) ATE295894T1 (en)
AU (1) AU7633094A (en)
DE (1) DE69434377T2 (en)
ES (1) ES2240965T3 (en)
HK (1) HK1013110A1 (en)
WO (1) WO1995005472A2 (en)

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US6946588B2 (en) 1996-03-13 2005-09-20 Monsanto Technology Llc Nucleic acid encoding a modified threonine deaminase and methods of use
US5958745A (en) * 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
US5959179A (en) * 1996-03-13 1999-09-28 Monsanto Company Method for transforming soybeans
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US5750848A (en) * 1996-08-13 1998-05-12 Monsanto Company DNA sequence useful for the production of polyhydroxyalkanoates
JP3062459B2 (en) * 1996-08-14 2000-07-10 理化学研究所 Polyester synthase gene and method for producing polyester
EP0977865A1 (en) * 1997-02-13 2000-02-09 James Madison University Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units
US6143561A (en) 1997-06-30 2000-11-07 The Curators Of The University Of Missouri DNA encoding plastid pyruvate dehydrogenase and branched chain oxoacid dehydrogenase components
WO1999014313A2 (en) * 1997-09-19 1999-03-25 Metabolix, Inc. Biological systems for manufacture of polyhydroxylalkanoate polymers containing 4-hydroxyacids
WO1999035278A1 (en) * 1998-01-05 1999-07-15 Monsanto Company Biosynthesis of medium chain length polyhydroxyalkanoates
AU748733B2 (en) 1998-01-07 2002-06-13 Metabolix, Inc. Animal nutrition compositions
WO1999036547A1 (en) * 1998-01-19 1999-07-22 Lg Chemical Ltd. POLYHYDROCYALKANOATE BIOSYNTHESIS-RELATED GENES DERIVED FROM $i(ALCALIGENES LATUS)
US6586658B1 (en) 1998-03-06 2003-07-01 Metabolix, Inc. Modification of fatty acid metabolism in plants
AU750057B2 (en) * 1998-03-06 2002-07-11 Metabolix, Inc. Modification of fatty acid metabolism in plants
US6143952A (en) * 1998-03-31 2000-11-07 Regents Of The University Of Minnesota Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
US6103956A (en) * 1998-03-31 2000-08-15 Regents Of The University Of Minnesota Polyhydroxyalkanoate synthesis in plants
MXPA00009754A (en) 1998-04-08 2002-05-08 Metabolix Inc Methods for separation and purification of biopolymers.
WO1999061624A2 (en) * 1998-05-22 1999-12-02 Metabolix, Inc. Polyhydroxyalkanoate biopolymer compositions
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
ATE317014T1 (en) * 1998-07-30 2006-02-15 Metabolix Inc BIFUNCTIONAL ENZYMES FOR BIOPOLYMER PRODUCTION
ATE385521T1 (en) 1998-08-04 2008-02-15 Metabolix Inc PRODUCTION OF POLYHYDROXYALKANOATES FROM POLYOLS
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US6160199A (en) * 1998-12-21 2000-12-12 The Procter & Gamble Company Absorbent articles comprising biodegradable PHA copolymers
US6174990B1 (en) 1998-12-21 2001-01-16 The Procter & Gamble Company Films comprising biodegradable PHA copolymers
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DK1161539T3 (en) 1999-03-15 2005-11-28 Univ Laval Process for preparing polyhydroxyalkanoates in recombinant organisms
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US6451564B1 (en) * 1999-07-02 2002-09-17 Massachusetts Institute Of Technology Methods for producing L-isoleucine
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Also Published As

Publication number Publication date
AU7633094A (en) 1995-03-14
HK1013110A1 (en) 1999-08-13
US5610041A (en) 1997-03-11
JPH09501832A (en) 1997-02-25
ES2240965T3 (en) 2005-10-16
EP0719342A1 (en) 1996-07-03
EP0719342B1 (en) 2005-05-18
WO1995005472A2 (en) 1995-02-23
DE69434377T2 (en) 2006-02-02
ATE295894T1 (en) 2005-06-15
DE69434377D1 (en) 2005-06-23

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