WO1995007095A1 - Injectable compositions for soft tissue augmentation - Google Patents

Injectable compositions for soft tissue augmentation Download PDF

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Publication number
WO1995007095A1
WO1995007095A1 PCT/US1994/010223 US9410223W WO9507095A1 WO 1995007095 A1 WO1995007095 A1 WO 1995007095A1 US 9410223 W US9410223 W US 9410223W WO 9507095 A1 WO9507095 A1 WO 9507095A1
Authority
WO
WIPO (PCT)
Prior art keywords
elastin
composition
tissue
collagen
soft tissue
Prior art date
Application number
PCT/US1994/010223
Other languages
French (fr)
Inventor
Ernst Janzen
Matthias Johannes Hoekstra
Richard P. Dutrieux
Original Assignee
Datascope Investment Corp.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Datascope Investment Corp. filed Critical Datascope Investment Corp.
Priority to DK94927397T priority Critical patent/DK0717634T3/en
Priority to JP1995508822A priority patent/JP3596892B6/en
Priority to AT94927397T priority patent/ATE210992T1/en
Priority to EP94927397A priority patent/EP0717634B1/en
Priority to CA002170754A priority patent/CA2170754C/en
Priority to KR1019960701112A priority patent/KR100333088B1/en
Priority to DE69429522T priority patent/DE69429522T2/en
Priority to AU76857/94A priority patent/AU675879B2/en
Publication of WO1995007095A1 publication Critical patent/WO1995007095A1/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/39Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14Macromolecular materials
    • A61L27/22Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14Macromolecular materials
    • A61L27/26Mixtures of macromolecular compounds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P41/00Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the present invention relates to injectable compositions having primary utility for soft tissue augmentation.
  • injectable compositions for soft tissue augmentation, especially breast augmentation.
  • breast augmentation has been on surgically implantable breast prostheses
  • injectable compositions offer the very important advantage of being able to avoid a surgical procedure.
  • the present invention addresses the problems encountered in the prior art by using injectable compositions containing non-cross linked collagen together with significant amounts of elastin.
  • Elastin has a much lower tendency than collagen toward resorption.
  • elastin attracts fibroblasts which in turn produce new native connective tissue.
  • elastin Another advantage of using elastin is that, in suspension, it has a lower viscosity and hence flows much more readily than collagen. This characteristic of elastin makes it easier to inject higher solids content compositions than is possible when using collagen alone.
  • compositions of the present invention are for breast augmentation.
  • Another cosmetic application is as a dermal implant to remove wrinkles, primarily around the face and neck.
  • compositions of the present invention can be used in connection with overcoming urinary incontinence. In this latter application, a composition according to the present invention would be injected into the area of the urethral sphincter.
  • compositions of the present invention include load-bearing tissue augmentation, for example, under a corn, and general and specific contour improvements, for example, for nose contour corrections and to correct viral pock marks and acne scars.
  • load bearing tissue augmentation the composition of the present invention would be injected between the load bearing tissue, for example the corn, and the load exerting medium, for example the bone.
  • the composition of the present invention would be injected into the soft tissue beneath the imperfection.
  • the composition of the present invention would be injected into the soft tissue of the nose.
  • the ligamentum nuchae is made up largely of elastin, with only a relatively small amount of collagen. Indeed, more than 70% of the dry weight of this ligament is elastin. Because of the relatively high elastin content and relatively low collagen content, it is an ideal starting material to use in making an injectable composition according to the present invention.
  • the ligamentum nuchae may be cleaned using a procedure similar to that taught in U.S.P. 5,028,695, the disclosure of which is incorporated herein by reference. Generally, it is first cleaned of blood and adherent tissue. It is then chemically treated to remove the non-elastinous and non-collagenous components. The chemical treatment generally involves first treating the ligament with a strong alkali solution, then with an acid, and then with a neutralizing agent. The alkali sequence may be repeated several times if desired. Chemical treatment is followed by mechanical working to separate the elastin fibers. The separated fibers are then suspended in a suitable biocompatible carrier, for example a mixture of water and glycerin.
  • a suitable biocompatible carrier for example a mixture of water and glycerin.
  • the preferred starting material is the ligamentum nuchae
  • other ligaments and tendons may also be used.
  • the peritoneum, omentum and other animal membranes, especially those which have significant amounts of elastin, could be used.
  • elastin and collagen from different sources could be mixed together to produce a mix having whatever proportions are deemed advantageous for a particular application. It is believed, however, that the composition should have a minimum of perhaps as little as about 10% elastin (dry weight) and might have as much as 90% or perhaps even higher of elastin.
  • the cross-linking agent should be one which forms strong stable bonds with the collagen so that the cross-linking agent does not reach out and so that the cross-linked collagen is non- cytotoxic and does not provoke an immune or cellular response.
  • cross-linking agents might include hexa ethylene diisocyanate, some polyepoxy compounds, for example, polyethylene glycol diglycidyl ether, and some water soluble carbodiimides, for example, 1-ethyl- 3 [3-dimethyl amino propyl] carbodiimide.HCl in the presence of N-hydroxysuccinimide.
  • bovine ligamentum nuchae weighing about 10 kg. was soaked overnight in about 40 1. of tap water at room temperature to remove adherent blood and other water soluble components. Soaking in water also assures a more or less natural degree of hydration which is believed to facilitate the subsequent chemical treatments.
  • the ligament was washed twice for about 10 minutes each, again in tap water, before being placed in 50 1. of 4% (w/w) solution of sodium hydroxide (NaOH) in tap water. It was permitted to remain in this strongly alkaline soak for 48 hrs. at room temperature.
  • the alkaline soak was followed by three 10 minute washes in 50 1. of tap water and was then subjected to a second alkaline soak, this one in a 2% (w/w) solution of NaOH in tap water at room temperature for 72 hrs. There then followed three more 10 minute washes in tap water to remove the solubilized components.
  • the ligament was placed in a solution of hydrochloric acid (HC1) for about 4 hrs.
  • HC1 solution for this soak was prepared by mixing 4 1. of concentrated (37%) HC1 with 36 1. of tap water.
  • the acid soaked ligament was then washed in tap water until the pH of the water was between about 2.5 and 3.
  • the ligament was placed in a sodium bicarbonate (NaHC0 3 ) soak to neutralize the remaining acid.
  • NaHC0 3 soak was prepared by adding 350 gm. of NaHC0 3 to 50 1. of tap water. The ligament was left in this neutralizing bath overnight and then it was again washed in tap water to remove the resulting salts. Washing continued until mixing with a silver nitrate (AgN0 3 ) solution produced no precipitates.
  • a kolloid mill was then used to break up the natural collagen/elastin matrix and separate the elastin fibers.
  • Acetone extractions were then used to remove the water and the fibers were then air dried in an oven at about 75° C.
  • the collagen in the composition according to this present invention acts as a stimulant during the initial phase after injection. It tends to cause some mild tissue reaction and increased vascular activity. Of course, this leads to resorption, but in the process, fibroblasts, which appear to have an affinity for elastin, invade the injected composition and attach themselves to the elastin fibers. Hence, these fibroblasts lay down an organized matrix of new native connective tissue. Eventually, the injected collagen has been resorbed and all that remains is the injected elastin in a new native connective tissue structure.

Abstract

Injectable implant compositions for soft tissue augmentation comprise elastin and collagen and a biocompatible carrier. An injectable implant composition for soft tissue augmentation is derived from the ligamentum nuchae which has been treated to remove non-collagenous and non-elastinous proteins. Methods of making an injectable implant composition for soft tissue augmentation from the ligamentum nuchae are described.

Description

TITLE
INJECTABLE COMPOSITIONS FOR SOFT TISSUE AUGMENTATION
BACKGROUND OF THE INVENTION
Field of the Invention
The present invention relates to injectable compositions having primary utility for soft tissue augmentation.
Related Background
Over the years, many attempts have been made to develop injectable compositions for soft tissue augmentation, especially breast augmentation. Although in recent years the emphasis in breast augmentation has been on surgically implantable breast prostheses, injectable compositions offer the very important advantage of being able to avoid a surgical procedure.
Some of the early attempts to make injectable compositions for breast augmentation involved the use of silicone gel. However, silicone injected subcutaneously has a tendency to migrate into the surrounding tissue causing, among other problems, granulomas. Accordingly, injectable liquid gels are no longer in use.
Subsequently, injectable collagen in suspension became the composition of choice. However, natural collagen has a great tendency to be resorbed. Therefore, in order to have soft tissue augmentation that did not dissipate shortly after injection, it was found necessary to cross link the collagen with agents such as glutaraldehyde. Cross linking inhibits resorption. However, recently the use of glutaraldehyde cross linked collagen has itself come under attack.
SUMMARY OF THE INVENTION The present invention addresses the problems encountered in the prior art by using injectable compositions containing non-cross linked collagen together with significant amounts of elastin. Elastin has a much lower tendency than collagen toward resorption. Moreover, it is believed that elastin attracts fibroblasts which in turn produce new native connective tissue. Thus, when a composition according to the present invention is injected subcutaneously into soft tissue, as the injected collagen is being resorbed, new native connective tissue is being generated, until a steady state situation has been achieved.
Another advantage of using elastin is that, in suspension, it has a lower viscosity and hence flows much more readily than collagen. This characteristic of elastin makes it easier to inject higher solids content compositions than is possible when using collagen alone.
As indicated above, one use for the compositions of the present invention is for breast augmentation. Another cosmetic application is as a dermal implant to remove wrinkles, primarily around the face and neck. In addition, there are medical applications as well. For example, compositions of the present invention can be used in connection with overcoming urinary incontinence. In this latter application, a composition according to the present invention would be injected into the area of the urethral sphincter.
Other possible uses for the compositions of the present invention include load-bearing tissue augmentation, for example, under a corn, and general and specific contour improvements, for example, for nose contour corrections and to correct viral pock marks and acne scars. For load bearing tissue augmentation, the composition of the present invention would be injected between the load bearing tissue, for example the corn, and the load exerting medium, for example the bone. To alleviate skin contour defects, such as viral infection pock marks and acne scars, the composition of the present invention would be injected into the soft tissue beneath the imperfection. Similarly, to change the contour of a person's nose, the composition of the present invention would be injected into the soft tissue of the nose.
While collagen has been used previously for the above- described applications, it has generally been in the form of cross-linked collagen. Even with cross-linked collagen, however, the resorption time is often unacceptably short, especially after repeated injections. Indeed, injections of cross-linked collagen must frequently be repeated several times over a short time span before adequate augmentation can be achieved. In addition, glutaraldehyde cross-linked collagen tends to cause tissue reactions in some patients and, also, there is some concern about potential immunological problems associated with the use of collagen that has been cross-linked with glutaraldehyde. Cross linking agents like glutaraldehyde are known to be toxic and there is concern that repeated injections of glutaraldehyde- containing compositions may produce progressively more severe immune reactions and increasingly rapid resorption. The compositions of the present invention, on the other hand, are believed to have much longer resorption times and to produce no significant immunological or tissue reaction problems.
Detailed Description
It is well known that the ligamentum nuchae is made up largely of elastin, with only a relatively small amount of collagen. Indeed, more than 70% of the dry weight of this ligament is elastin. Because of the relatively high elastin content and relatively low collagen content, it is an ideal starting material to use in making an injectable composition according to the present invention.
To make a preferred injectable composition according to the present invention, the ligamentum nuchae may be cleaned using a procedure similar to that taught in U.S.P. 5,028,695, the disclosure of which is incorporated herein by reference. Generally, it is first cleaned of blood and adherent tissue. It is then chemically treated to remove the non-elastinous and non-collagenous components. The chemical treatment generally involves first treating the ligament with a strong alkali solution, then with an acid, and then with a neutralizing agent. The alkali sequence may be repeated several times if desired. Chemical treatment is followed by mechanical working to separate the elastin fibers. The separated fibers are then suspended in a suitable biocompatible carrier, for example a mixture of water and glycerin.
Although the preferred starting material is the ligamentum nuchae, other ligaments and tendons may also be used. For example, the peritoneum, omentum and other animal membranes, especially those which have significant amounts of elastin, could be used. Also, elastin and collagen from different sources could be mixed together to produce a mix having whatever proportions are deemed advantageous for a particular application. It is believed, however, that the composition should have a minimum of perhaps as little as about 10% elastin (dry weight) and might have as much as 90% or perhaps even higher of elastin.
While, as noted above, it is believed preferable not to use any cross-linking agents, there may be applications where cross-linking can be tolerated or might even be desirable. In that event, the cross-linking agent should be one which forms strong stable bonds with the collagen so that the cross-linking agent does not reach out and so that the cross-linked collagen is non- cytotoxic and does not provoke an immune or cellular response. Such cross-linking agents might include hexa ethylene diisocyanate, some polyepoxy compounds, for example, polyethylene glycol diglycidyl ether, and some water soluble carbodiimides, for example, 1-ethyl- 3 [3-dimethyl amino propyl] carbodiimide.HCl in the presence of N-hydroxysuccinimide.
Example
A portion of bovine ligamentum nuchae weighing about 10 kg. was soaked overnight in about 40 1. of tap water at room temperature to remove adherent blood and other water soluble components. Soaking in water also assures a more or less natural degree of hydration which is believed to facilitate the subsequent chemical treatments.
After the initial soak, the ligament was washed twice for about 10 minutes each, again in tap water, before being placed in 50 1. of 4% (w/w) solution of sodium hydroxide (NaOH) in tap water. It was permitted to remain in this strongly alkaline soak for 48 hrs. at room temperature.
The alkaline soak was followed by three 10 minute washes in 50 1. of tap water and was then subjected to a second alkaline soak, this one in a 2% (w/w) solution of NaOH in tap water at room temperature for 72 hrs. There then followed three more 10 minute washes in tap water to remove the solubilized components.
After the second alkaline soak and subsequent washes, the ligament was placed in a solution of hydrochloric acid (HC1) for about 4 hrs. The HC1 solution for this soak was prepared by mixing 4 1. of concentrated (37%) HC1 with 36 1. of tap water. The acid soaked ligament was then washed in tap water until the pH of the water was between about 2.5 and 3.
At that point, the ligament was placed in a sodium bicarbonate (NaHC03) soak to neutralize the remaining acid. The NaHC03 soak was prepared by adding 350 gm. of NaHC03 to 50 1. of tap water. The ligament was left in this neutralizing bath overnight and then it was again washed in tap water to remove the resulting salts. Washing continued until mixing with a silver nitrate (AgN03) solution produced no precipitates.
A kolloid mill was then used to break up the natural collagen/elastin matrix and separate the elastin fibers. Acetone extractions were then used to remove the water and the fibers were then air dried in an oven at about 75° C.
Finally, 150 gm. of the dried fibers were suspended in 2 1. of a water/glycerine mixture comprised of 1 1. water and 1 1. glycerine. This suspension was then ready for use in accordance with the present invention.
It is believed that the collagen in the composition according to this present invention acts as a stimulant during the initial phase after injection. It tends to cause some mild tissue reaction and increased vascular activity. Of course, this leads to resorption, but in the process, fibroblasts, which appear to have an affinity for elastin, invade the injected composition and attach themselves to the elastin fibers. Hence, these fibroblasts lay down an organized matrix of new native connective tissue. Eventually, the injected collagen has been resorbed and all that remains is the injected elastin in a new native connective tissue structure.
It will readily become apparent to those skilled in this art that numerous modifications, alterations and changes can be made with respect to the specifics of the above description without departing from the inventive concept described herein. Accordingly, all such variants should be viewed as being within the scope of the invention as set forth in the claims below.

Claims

WHAT IS CLAIMED IS:
1. An injectable implant composition for soft tissue augmentation comprising elastin and collagen and a biocompatible carrier.
2. A composition according to claim 1 wherein said elastin is at least 10% by weight of the combined dry weight of elastin plus collagen.
3. A composition according to claim 1 wherein said elastin content is at least 50% of said combined dry weight.
4. A composition according to claim 1 wherein said elastin content is at least 70% of said combined weight.
5. A composition according to claim l wherein said elastin and said collagen are derived, at least in part, from different tissue sources.
6. A composition according to claim 1 wherein said collagen and elastin have been treated to remove non- collagenous and non-elastinous proteins.
7. A composition according to claim 1 wherein said collagen has not been cross-linked.
8. A composition according to claim 1 wherein said collagen has been cross-linked with a compound selected from the group consisting of hexamethylene diiosocyanate, a polyepoxy and a water soluble carbodiimide.
9. An injectable implant composition for soft tissue augmentation derived from the ligamentum nuchae which has been treated to remove non-collagenous and non- elastinous proteins.
10. A method of treating urinary incontinence comprising injecting a composition in accordance with claim 1 into the area of the urethral sphincter.
11. A method of breast augmentation comprising injecting a composition in accordance with claim 1 into the breast.
12. A method of cosmetic treatment of wrinkles comprising injecting a composition in accordance with claim 1 into the soft tissue in or around the face.
13. A method of load bearing tissue augmentation comprising injecting a composition in accordance with claim 1 between said load bearing tissue and the load- exerting medium.
14. A method of treating acne scars or viral pock marks comprising injecting a composition in accordance with claim 1 into the soft tissue underlying said scar or pock mark.
15. A method of changing the contours of a nose comprising injecting a composition in accordance with claim 1 into the soft tissue of the nose.
16. A method of making an injectable implant composition for soft tissue augmentation from the ligamentum nuchae comprising the steps of washing the ligamentum nuchae in water, treating the washed ligamentum nuchae with a strong alkali solution, treating the ligamentum nuchae with an acid, treating the ligamentum nuchae with a base to neutralize the acid, mechanically working the ligamentum nuchae to separate the elastin fibers, suspending at least some of the collagen and elastin fibers in a suitable carrier.
17. A method of making an injectable implant composition for soft tissue augmentation comprising the steps of selecting tissue starting material from the group consisting of animal ligaments, tendons and membranes, washing said tissue in water, treating the washed tissue with a strong alkali solution, treating the tissue with an acid, treating the tissue with a base to neutralize the acid, mechanically working the tissue to separate the elastin fibers, suspending at least some of the elastin fibers in a suitable carrier.
PCT/US1994/010223 1993-09-07 1994-09-07 Injectable compositions for soft tissue augmentation WO1995007095A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DK94927397T DK0717634T3 (en) 1993-09-07 1994-09-07 Injectable soft tissue enlargement compositions
JP1995508822A JP3596892B6 (en) 1993-09-07 1994-09-07 Injectable composition for soft tissue augmentation
AT94927397T ATE210992T1 (en) 1993-09-07 1994-09-07 INJECTABLE COMPOSITIONS FOR ENLARGING SOFT TISSUE
EP94927397A EP0717634B1 (en) 1993-09-07 1994-09-07 Injectable compositions for soft tissue augmentation
CA002170754A CA2170754C (en) 1993-09-07 1994-09-07 Injectable compositions for soft tissue augmentation
KR1019960701112A KR100333088B1 (en) 1993-09-07 1994-09-07 Injectable compositions for soft tissue expansion
DE69429522T DE69429522T2 (en) 1993-09-07 1994-09-07 INJECTABLE COMPOSITIONS TO ENLARGE SOFT TISSUE
AU76857/94A AU675879B2 (en) 1993-09-07 1994-09-07 Injectable compositions for soft tissue augmentation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/116,517 1993-09-07
US08/116,517 US5523291A (en) 1993-09-07 1993-09-07 Injectable compositions for soft tissue augmentation

Publications (1)

Publication Number Publication Date
WO1995007095A1 true WO1995007095A1 (en) 1995-03-16

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ID=22367654

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/010223 WO1995007095A1 (en) 1993-09-07 1994-09-07 Injectable compositions for soft tissue augmentation

Country Status (13)

Country Link
US (2) US5523291A (en)
EP (1) EP0717634B1 (en)
KR (1) KR100333088B1 (en)
CN (1) CN1142789C (en)
AT (1) ATE210992T1 (en)
AU (1) AU675879B2 (en)
BR (1) BR9407420A (en)
CA (1) CA2170754C (en)
DE (1) DE69429522T2 (en)
DK (1) DK0717634T3 (en)
ES (1) ES2169085T3 (en)
PT (1) PT717634E (en)
WO (1) WO1995007095A1 (en)

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US5705488A (en) 1998-01-06
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JP3596892B2 (en) 2004-12-02
ATE210992T1 (en) 2002-01-15

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