WO1996028556A3 - Proteinase k variants having decreased adsorption and increased hydrolysis - Google Patents

Proteinase k variants having decreased adsorption and increased hydrolysis Download PDF

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Publication number
WO1996028556A3
WO1996028556A3 PCT/US1996/003005 US9603005W WO9628556A3 WO 1996028556 A3 WO1996028556 A3 WO 1996028556A3 US 9603005 W US9603005 W US 9603005W WO 9628556 A3 WO9628556 A3 WO 9628556A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteinase
variants
loop region
wild
acid sequence
Prior art date
Application number
PCT/US1996/003005
Other languages
French (fr)
Other versions
WO1996028556A2 (en
Inventor
Philip Frederick Brode Iii
Bobby Lee Barnett
Donn Nelton Rubingh
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Priority to EP96907182A priority Critical patent/EP0813601A2/en
Priority to MX9706841A priority patent/MX9706841A/en
Priority to BR9607677A priority patent/BR9607677A/en
Priority to JP8527690A priority patent/JPH11501814A/en
Priority to CA002214395A priority patent/CA2214395C/en
Priority to AU50927/96A priority patent/AU5092796A/en
Publication of WO1996028556A2 publication Critical patent/WO1996028556A2/en
Publication of WO1996028556A3 publication Critical patent/WO1996028556A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/0047—Detergents in the form of bars or tablets
    • C11D17/0065—Solid detergents containing builders
    • C11D17/0073—Tablets
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/0047—Detergents in the form of bars or tablets
    • C11D17/0065—Solid detergents containing builders
    • C11D17/0069—Laundry bars
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/0047—Detergents in the form of bars or tablets
    • C11D17/0065—Solid detergents containing builders
    • C11D17/0073—Tablets
    • C11D17/0078—Multilayered tablets
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/0005—Other compounding ingredients characterised by their effect
    • C11D3/0052—Gas evolving or heat producing compositions
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/0005—Other compounding ingredients characterised by their effect
    • C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Abstract

The present invention relates to proteinase K variants having a modified amino acid sequence of wild-type proteinase K amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type proteinase K (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the proteinase K variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type proteinase K. The present invention also relates to DNA sequences encoding such proteinase K variants. The present invention also relates to compositions comprising such proteinase K variants for cleaning a variety of surfaces.
PCT/US1996/003005 1995-03-09 1996-03-06 Proteinase k variants having decreased adsorption and increased hydrolysis WO1996028556A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP96907182A EP0813601A2 (en) 1995-03-09 1996-03-06 Proteinase k variants having decreased adsorption and increased hydrolysis
MX9706841A MX9706841A (en) 1995-03-09 1996-03-06 Proteinase k variants having decreased adsorption and increased hydrolysis.
BR9607677A BR9607677A (en) 1995-03-09 1996-03-06 Porteinase k variants having reduced adsorption and increased hydrolysis
JP8527690A JPH11501814A (en) 1995-03-09 1996-03-06 Proteinase K variants with reduced adsorptivity and increased hydrolyzability
CA002214395A CA2214395C (en) 1995-03-09 1996-03-06 Proteinase k variants having decreased adsorption and increased hydrolysis
AU50927/96A AU5092796A (en) 1995-03-09 1996-03-06 Proteinase k variants having decreased adsorption and increased hydrolysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40157495A 1995-03-09 1995-03-09
US08/401,574 1995-03-09

Publications (2)

Publication Number Publication Date
WO1996028556A2 WO1996028556A2 (en) 1996-09-19
WO1996028556A3 true WO1996028556A3 (en) 1997-07-17

Family

ID=23588288

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/003005 WO1996028556A2 (en) 1995-03-09 1996-03-06 Proteinase k variants having decreased adsorption and increased hydrolysis

Country Status (14)

Country Link
US (1) US6451574B1 (en)
EP (1) EP0813601A2 (en)
JP (1) JPH11501814A (en)
CN (1) CN1183809A (en)
AU (1) AU5092796A (en)
BR (1) BR9607677A (en)
CA (1) CA2214395C (en)
CZ (1) CZ279997A3 (en)
HU (1) HUP9801638A3 (en)
IL (1) IL117350A0 (en)
MA (1) MA23820A1 (en)
MX (1) MX9706841A (en)
WO (1) WO1996028556A2 (en)
ZA (1) ZA961820B (en)

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AR016969A1 (en) * 1997-10-23 2001-08-01 Procter & Gamble PROTEASE VARIANTE, ADN, EXPRESSION VECTOR, GUEST MICROORGANISM, CLEANING COMPOSITION, ANIMAL FOOD AND COMPOSITION TO TREAT A TEXTILE
EP1045892A1 (en) * 1997-12-22 2000-10-25 The Procter & Gamble Company Cleaning compositions containing a neopullulanase
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US7943385B2 (en) 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
WO2009140343A1 (en) * 2008-05-13 2009-11-19 General Atomics Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
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CN108603187A (en) 2015-11-24 2018-09-28 诺维信公司 Polypeptide with proteinase activity and encode their polynucleotides
CN109280656B (en) * 2018-10-23 2021-06-15 大连博格林生物科技有限公司 Recombinant beauveria bassiana proteinase K mutant PK-M1 and preparation method thereof
CN109207460B (en) * 2018-10-23 2021-06-15 大连博格林生物科技有限公司 Recombinant beauveria bassiana proteinase K mutant PK-M2 and preparation method thereof
US20200199565A1 (en) * 2018-12-20 2020-06-25 New England Biolabs, Inc. Proteinases with Improved Properties
CN112592931A (en) * 2020-12-31 2021-04-02 安徽丰原发酵技术工程研究有限公司 Method for producing recombinant proteinase K
CN113913412B (en) * 2021-10-13 2023-10-03 湖北大学 Proteinase K mutant and its preparing process
CN114196656B (en) * 2021-12-29 2023-10-24 南京巨匠生物科技有限公司 Proteinase K mutant and construction and application of its expression vector
CN115775591B (en) * 2023-01-10 2023-06-09 华测检测认证集团股份有限公司 Primer design method, device, equipment and readable storage medium

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Also Published As

Publication number Publication date
BR9607677A (en) 1998-07-07
WO1996028556A2 (en) 1996-09-19
CA2214395C (en) 2001-10-30
MX9706841A (en) 1997-11-29
IL117350A0 (en) 1996-07-23
EP0813601A2 (en) 1997-12-29
CN1183809A (en) 1998-06-03
CA2214395A1 (en) 1996-09-19
MA23820A1 (en) 1996-10-01
AU5092796A (en) 1996-10-02
ZA961820B (en) 1996-09-10
CZ279997A3 (en) 1998-01-14
US6451574B1 (en) 2002-09-17
HUP9801638A2 (en) 1998-10-28
HUP9801638A3 (en) 2001-01-29
JPH11501814A (en) 1999-02-16

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