WO1998010097A3 - Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination - Google Patents

Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination Download PDF

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Publication number
WO1998010097A3
WO1998010097A3 PCT/US1997/013938 US9713938W WO9810097A3 WO 1998010097 A3 WO1998010097 A3 WO 1998010097A3 US 9713938 W US9713938 W US 9713938W WO 9810097 A3 WO9810097 A3 WO 9810097A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecular weight
target molecule
target
sample
concentration
Prior art date
Application number
PCT/US1997/013938
Other languages
French (fr)
Other versions
WO1998010097A2 (en
Inventor
Alonso Castro
John Williams
Original Assignee
Pioneer Hi Bred Int
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Univ California filed Critical Pioneer Hi Bred Int
Priority to AU38291/97A priority Critical patent/AU3829197A/en
Publication of WO1998010097A2 publication Critical patent/WO1998010097A2/en
Publication of WO1998010097A3 publication Critical patent/WO1998010097A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Abstract

The present invention comprises a novel method for detecting the presence of a target molecule in a sample and determining its molecular weight and concentration. The method involves the determination of electrokinetic velocities by measuring the time required for individual molecules labeled with at least two fluorescent probes to travel a fixed distance between two laser beams. Comparison of the electrophoretic velocity known to be characteristic of particular molecular weight standards with that of the target species permits determination of the molecular weight of the target. Alternatively, a two probe single detection zone method utilizing mechanical, electroosmotic or electrokinetic flow of a sample through a transport tube permits the identification of a target molecule in a complex test sample. The method is extremely sensitive, allowing one to detect and determine in a matter of seconds the molecular weight of a target molecule present in a concentration as small as one femtomolar.
PCT/US1997/013938 1996-09-03 1997-08-08 Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination WO1998010097A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU38291/97A AU3829197A (en) 1996-09-03 1997-08-08 Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70946896A 1996-09-03 1996-09-03
US08/709,468 1996-09-03

Publications (2)

Publication Number Publication Date
WO1998010097A2 WO1998010097A2 (en) 1998-03-12
WO1998010097A3 true WO1998010097A3 (en) 2002-10-17

Family

ID=24849984

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/013938 WO1998010097A2 (en) 1996-09-03 1997-08-08 Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination

Country Status (4)

Country Link
AR (1) AR009477A1 (en)
AU (1) AU3829197A (en)
CR (1) CR5602A (en)
WO (1) WO1998010097A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69825601T2 (en) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline METHOD FOR THE ANALYSIS OF POLYMERS
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6927065B2 (en) * 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US20030124592A1 (en) * 2001-10-24 2003-07-03 Bioprofile, Llc Methods for detecting genetic haplotypes by interaction with probes
US7282330B2 (en) 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US7371520B2 (en) 2002-05-28 2008-05-13 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
EP1586068B1 (en) * 2003-01-23 2008-07-30 U.S. Genomics, Inc. Methods for analyzing polymer populations
JP2007518107A (en) 2004-01-13 2007-07-05 ユー．エス． ジェノミクス， インコーポレイテッド Detection and quantification of analytes in solution using polymers
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7351538B2 (en) 2004-08-23 2008-04-01 U.S. Genomics Systems and methods for detecting and analyzing polymers
WO2006098772A2 (en) 2004-10-13 2006-09-21 U.S. Genomics, Inc. Systems and methods for measurement optimization
US8045140B2 (en) * 2007-10-18 2011-10-25 Stc.Unm Method for multivariate analysis of confocal temporal image sequences for velocity estimation
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample

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WO1993019205A1 (en) * 1992-03-19 1993-09-30 The Regents Of The University Of California Multiple tag labeling method for dna sequencing
WO1993020236A1 (en) * 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
WO1996006189A1 (en) * 1994-08-24 1996-02-29 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
EP0724153A1 (en) * 1994-07-13 1996-07-31 Hitachi Electronics Engineering Co., Ltd. DNA Base sequencer
EP0731173A2 (en) * 1995-03-10 1996-09-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Berlin Method for the direct detection of a few oligonucleotides
WO1997012995A1 (en) * 1995-10-06 1997-04-10 Perseptive Biosystems, Inc. Methods and kit for hybridization analysis using peptide nucleic acid probes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993019205A1 (en) * 1992-03-19 1993-09-30 The Regents Of The University Of California Multiple tag labeling method for dna sequencing
WO1993020236A1 (en) * 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
EP0724153A1 (en) * 1994-07-13 1996-07-31 Hitachi Electronics Engineering Co., Ltd. DNA Base sequencer
WO1996006189A1 (en) * 1994-08-24 1996-02-29 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
EP0731173A2 (en) * 1995-03-10 1996-09-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Berlin Method for the direct detection of a few oligonucleotides
WO1997012995A1 (en) * 1995-10-06 1997-04-10 Perseptive Biosystems, Inc. Methods and kit for hybridization analysis using peptide nucleic acid probes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CASTRO AND WILLIAMS: "SINGLE-MOLECULE DETECTION OF SPECIFIC NUCLEIC ACID SEQUENCES IN UNAMPLIFIED GENOMIC DNA", ANALYTICAL CHEMISTRY, vol. 69, no. 19, 1997, pages 3915 - 3920, XP002046358 *
CHEN J W ET AL: "IDENTIFICATION OF DNA MOLECULES BY PRE-COLUMN HYBRIDIZATION USING CAPILLARY ELECTROPHORESIS", JOURNAL OF CHROMATOGRAPHY, vol. 559, 18 October 1991 (1991-10-18), pages 295 - 305, XP000615726 *
EIGEN AND RIGLER: "SORTING SINGLE MOLECULES: APPLICATION TO DIAGNOSTICS AND EVOLUTIONARY BIOTECHNOLOGY", PROC. NATL. ACAD. SCI. USA, vol. 91, 1994, pages 5740 - 5747, XP002046666 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Also Published As

Publication number Publication date
WO1998010097A2 (en) 1998-03-12
CR5602A (en) 1998-08-31
AR009477A1 (en) 2000-04-26
AU3829197A (en) 1998-03-26

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