WO1998013513A3 - Methods and compositions for identifying receptor effectors - Google Patents

Methods and compositions for identifying receptor effectors Download PDF

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Publication number
WO1998013513A3
WO1998013513A3 PCT/US1997/017159 US9717159W WO9813513A3 WO 1998013513 A3 WO1998013513 A3 WO 1998013513A3 US 9717159 W US9717159 W US 9717159W WO 9813513 A3 WO9813513 A3 WO 9813513A3
Authority
WO
WIPO (PCT)
Prior art keywords
identifying
compositions
methods
cellular protein
screening
Prior art date
Application number
PCT/US1997/017159
Other languages
French (fr)
Other versions
WO1998013513A2 (en
Inventor
Joshua Trueheart
Jeremy I Paul
Hans A Fuernkranz
Debra Nathan
Scott Holmes
Original Assignee
Cadus Pharmaceutical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadus Pharmaceutical Corp filed Critical Cadus Pharmaceutical Corp
Priority to CA002266543A priority Critical patent/CA2266543C/en
Priority to AT97944430T priority patent/ATE284972T1/en
Priority to DE69731960T priority patent/DE69731960T2/en
Priority to AU45930/97A priority patent/AU733007B2/en
Priority to EP97944430A priority patent/EP0929691B1/en
Priority to JP10515880A priority patent/JP2001501097A/en
Publication of WO1998013513A2 publication Critical patent/WO1998013513A2/en
Publication of WO1998013513A3 publication Critical patent/WO1998013513A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

The present invention makes available a rapid, effective assay for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. The subject assay is particularly amenable for identifying surrogate ligands for receptors especially from small molecule or peptide libraries or from peptides produced by an autocrine system.
PCT/US1997/017159 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors WO1998013513A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002266543A CA2266543C (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors
AT97944430T ATE284972T1 (en) 1996-09-24 1997-09-24 METHODS AND COMPOSITIONS FOR IDENTIFYING RECEPTOR EFFECTORS
DE69731960T DE69731960T2 (en) 1996-09-24 1997-09-24 METHOD AND COMPOSITIONS FOR IDENTIFYING RECEPTOR EFFECTORS
AU45930/97A AU733007B2 (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors
EP97944430A EP0929691B1 (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors
JP10515880A JP2001501097A (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US71891096A 1996-09-24 1996-09-24
US08/718,910 1996-09-24
US85146997A 1997-05-05 1997-05-05
US08/851,469 1997-05-05

Publications (2)

Publication Number Publication Date
WO1998013513A2 WO1998013513A2 (en) 1998-04-02
WO1998013513A3 true WO1998013513A3 (en) 1998-09-03

Family

ID=27109999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/017159 WO1998013513A2 (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors

Country Status (8)

Country Link
US (1) US6159705A (en)
EP (1) EP0929691B1 (en)
JP (1) JP2001501097A (en)
AT (1) ATE284972T1 (en)
AU (1) AU733007B2 (en)
CA (1) CA2266543C (en)
DE (1) DE69731960T2 (en)
WO (1) WO1998013513A2 (en)

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Also Published As

Publication number Publication date
AU733007B2 (en) 2001-05-03
US6159705A (en) 2000-12-12
JP2001501097A (en) 2001-01-30
DE69731960T2 (en) 2005-12-15
AU4593097A (en) 1998-04-17
WO1998013513A2 (en) 1998-04-02
ATE284972T1 (en) 2005-01-15
CA2266543C (en) 2003-12-09
CA2266543A1 (en) 1998-04-02
EP0929691B1 (en) 2004-12-15
EP0929691A2 (en) 1999-07-21
DE69731960D1 (en) 2005-01-20

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