WO1999040226A3 - Determination of a genotype of an amplification product at multiple allelic sites - Google Patents

Determination of a genotype of an amplification product at multiple allelic sites Download PDF

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Publication number
WO1999040226A3
WO1999040226A3 PCT/US1999/000499 US9900499W WO9940226A3 WO 1999040226 A3 WO1999040226 A3 WO 1999040226A3 US 9900499 W US9900499 W US 9900499W WO 9940226 A3 WO9940226 A3 WO 9940226A3
Authority
WO
WIPO (PCT)
Prior art keywords
allelic
target sequence
different
probes
fluorescer
Prior art date
Application number
PCT/US1999/000499
Other languages
French (fr)
Other versions
WO1999040226A2 (en
Inventor
Kenneth J Livak
Federico Goodsaid
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Priority to AT99903026T priority Critical patent/ATE469982T1/en
Priority to JP2000530635A priority patent/JP4388694B2/en
Priority to AU23144/99A priority patent/AU758463B2/en
Priority to CA2318880A priority patent/CA2318880C/en
Priority to DE69942444T priority patent/DE69942444D1/en
Priority to EP99903026A priority patent/EP1053348B1/en
Publication of WO1999040226A2 publication Critical patent/WO1999040226A2/en
Publication of WO1999040226A3 publication Critical patent/WO1999040226A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

A method is provided for genotyping a target sequence at at least two allelic sites by a 5' nuclease amplification reaction. In one embodiment, the method includes performing a nucleic acid amplification on a target sequence having at least two different allelic sites using a nucleic acid polymerase having 5'→3' nuclease activity and a primer capable of hybridizing to the target sequence in the presence of two or more sets of allelic oligonucleotide probes wherein: each set of allelic oligonucleotide probes is for detecting a different allelic site of the target sequence, each set of allelic oligonucleotide probes includes two or more probes which are complementary to different allelic variants at the allelic site being detected by the set of probes, the allelic site being 5' relative to a sequence to which the primer hybridizes to the target sequence, and at least all but one of the allelic oligonucleotide probes include a different fluorescer than the other probes and a quencher positioned on the probe to quench the fluorescence of the fluorescer; detecting a fluorescence spectrum of the amplification; calculating a fluorescence contribution of each fluorescer to the fluorescence spectrum; and determining a presence or absence of the different allelic variants at the two or more different allelic sites based on the fluorescence contribution of each fluorescer to the combined fluorescence spectrum.
PCT/US1999/000499 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites WO1999040226A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AT99903026T ATE469982T1 (en) 1998-02-04 1999-01-08 DETERMINATION OF THE GENOTYPE OF AN AMPLIFICATION PRODUCT AT MULTIPLE ALLELE SITES
JP2000530635A JP4388694B2 (en) 1998-02-04 1999-01-08 Genotyping of amplification products at multiple allele sites
AU23144/99A AU758463B2 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites
CA2318880A CA2318880C (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites
DE69942444T DE69942444D1 (en) 1998-02-04 1999-01-08 DETERMINATION OF THE GENOTYP OF AN AMPLIFICATION PRODUCT AT SEVERAL ALLELEN FACES
EP99903026A EP1053348B1 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1859598A 1998-02-04 1998-02-04
US09/018,595 1998-02-04

Publications (2)

Publication Number Publication Date
WO1999040226A2 WO1999040226A2 (en) 1999-08-12
WO1999040226A3 true WO1999040226A3 (en) 2000-08-17

Family

ID=21788757

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/000499 WO1999040226A2 (en) 1998-02-04 1999-01-08 Determination of a genotype of an amplification product at multiple allelic sites

Country Status (8)

Country Link
US (5) US6154707A (en)
EP (1) EP1053348B1 (en)
JP (1) JP4388694B2 (en)
AT (1) ATE469982T1 (en)
AU (1) AU758463B2 (en)
CA (1) CA2318880C (en)
DE (1) DE69942444D1 (en)
WO (1) WO1999040226A2 (en)

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US9540515B2 (en) 2003-11-14 2017-01-10 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
US9206478B2 (en) 2002-12-31 2015-12-08 Branhaven LLC Methods and systems for inferring bovine traits
US9540515B2 (en) 2003-11-14 2017-01-10 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US9234233B2 (en) 2010-03-26 2016-01-12 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US9506059B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds

Also Published As

Publication number Publication date
US20020164630A1 (en) 2002-11-07
DE69942444D1 (en) 2010-07-15
EP1053348A2 (en) 2000-11-22
US7132239B2 (en) 2006-11-07
US20080187913A1 (en) 2008-08-07
CA2318880A1 (en) 1999-08-12
AU758463B2 (en) 2003-03-20
US20120053069A1 (en) 2012-03-01
CA2318880C (en) 2010-07-27
ATE469982T1 (en) 2010-06-15
US6884583B2 (en) 2005-04-26
EP1053348B1 (en) 2010-06-02
US20040053302A1 (en) 2004-03-18
JP2002502615A (en) 2002-01-29
JP4388694B2 (en) 2009-12-24
US6154707A (en) 2000-11-28
AU2314499A (en) 1999-08-23
WO1999040226A2 (en) 1999-08-12

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