WO2000001713A3 - Diacylglycerol acyl transferase proteins - Google Patents

Diacylglycerol acyl transferase proteins Download PDF

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Publication number
WO2000001713A3
WO2000001713A3 PCT/US1999/015243 US9915243W WO0001713A3 WO 2000001713 A3 WO2000001713 A3 WO 2000001713A3 US 9915243 W US9915243 W US 9915243W WO 0001713 A3 WO0001713 A3 WO 0001713A3
Authority
WO
WIPO (PCT)
Prior art keywords
dagat
proteins
diacylglycerol
acyl transferase
methods
Prior art date
Application number
PCT/US1999/015243
Other languages
French (fr)
Other versions
WO2000001713A2 (en
WO2000001713A9 (en
Inventor
Kathryn Dennis Lardizabal
Deborah Hawkins
Gregory A Thompson
Original Assignee
Calgene Llc
Kathryn Dennis Lardizabal
Deborah Hawkins
Gregory A Thompson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc, Kathryn Dennis Lardizabal, Deborah Hawkins, Gregory A Thompson filed Critical Calgene Llc
Priority to BR9911800-9A priority Critical patent/BR9911800A/en
Priority to CA2331329A priority patent/CA2331329C/en
Priority to EP99932285A priority patent/EP1098962B1/en
Priority to JP2000558114A priority patent/JP4514952B2/en
Priority to AT99932285T priority patent/ATE442435T1/en
Priority to DE69941390T priority patent/DE69941390D1/en
Publication of WO2000001713A2 publication Critical patent/WO2000001713A2/en
Publication of WO2000001713A9 publication Critical patent/WO2000001713A9/en
Publication of WO2000001713A3 publication Critical patent/WO2000001713A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The invention provides diacylglycerol acyl transferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.
PCT/US1999/015243 1998-07-02 1999-06-30 Diacylglycerol acyl transferase proteins WO2000001713A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BR9911800-9A BR9911800A (en) 1998-07-02 1999-06-30 Diaglycerol glycerol acyl transferase proteins
CA2331329A CA2331329C (en) 1998-07-02 1999-06-30 Diacylglycerol acyl transferase proteins
EP99932285A EP1098962B1 (en) 1998-07-02 1999-06-30 Diacylglycerol acyl transferase proteins
JP2000558114A JP4514952B2 (en) 1998-07-02 1999-06-30 Diacylglycerol acyltransferase protein
AT99932285T ATE442435T1 (en) 1998-07-02 1999-06-30 DIACYLGLYCEROL-ACYLTRANSFERASE PROTEINS
DE69941390T DE69941390D1 (en) 1998-07-02 1999-06-30 DIACYLGLYZERINE ACYLTRANSFERASE PROTEINS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9163198P 1998-07-02 1998-07-02
US60/091,631 1998-07-02
US13082999P 1999-04-23 1999-04-23
US60/130,829 1999-04-23

Publications (3)

Publication Number Publication Date
WO2000001713A2 WO2000001713A2 (en) 2000-01-13
WO2000001713A9 WO2000001713A9 (en) 2000-04-20
WO2000001713A3 true WO2000001713A3 (en) 2000-11-16

Family

ID=26784169

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/015243 WO2000001713A2 (en) 1998-07-02 1999-06-30 Diacylglycerol acyl transferase proteins

Country Status (8)

Country Link
US (1) US6822141B2 (en)
EP (1) EP1098962B1 (en)
JP (1) JP4514952B2 (en)
AT (1) ATE442435T1 (en)
BR (1) BR9911800A (en)
CA (1) CA2331329C (en)
DE (1) DE69941390D1 (en)
WO (1) WO2000001713A2 (en)

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DATABASE EMBL 3 July 1995 (1995-07-03), HILLIER ET AL.: "...Homo sapiens cDNA clone IMAGE:488805...", XP002146442 *
DATABASE EMBL 30 March 1998 (1998-03-30), MARRA ET AL.: "...Mus musculus cDNA clone IMAGE:1276709...", XP002146443 *
DATABASE EMBL 30 March 1998 (1998-03-30), MARRA ET AL.: "...Mus musculus cDNA clone IMAGE:1277098...", XP002146445 *
DATABASE EMBL 9 July 1996 (1996-07-09), BOYER ET AL.: "S.cerevisiae chromosome XV reading frame ORF YOR245c", XP002146447 *
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LITTLE ET AL: "Solubilization and characterization of diacylglycerol acyltransferase from microspore-derived cltures of oilseed rape", BIOCHEMICAL JOURNAL,GB,PORTLAND PRESS, LONDON, no. 304, 1 January 1994 (1994-01-01), pages 951 - 958, XP002079126, ISSN: 0264-6021 *

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