WO2000008208A3 - Reverse transcription and amplification processes and primers therefor - Google Patents
Reverse transcription and amplification processes and primers therefor Download PDFInfo
- Publication number
- WO2000008208A3 WO2000008208A3 PCT/GB1999/002579 GB9902579W WO0008208A3 WO 2000008208 A3 WO2000008208 A3 WO 2000008208A3 GB 9902579 W GB9902579 W GB 9902579W WO 0008208 A3 WO0008208 A3 WO 0008208A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- heeled
- reverse transcription
- primers
- amplification processes
- primers therefor
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU52943/99A AU5294399A (en) | 1998-08-05 | 1999-08-05 | Reverse transcription and amplification processes and primers therefor |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9817055.8A GB9817055D0 (en) | 1998-08-05 | 1998-08-05 | Reverse transcription and amplification processes and primers therefore |
GB9817055.8 | 1998-08-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000008208A2 WO2000008208A2 (en) | 2000-02-17 |
WO2000008208A3 true WO2000008208A3 (en) | 2000-05-11 |
Family
ID=10836764
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1999/002579 WO2000008208A2 (en) | 1998-08-05 | 1999-08-05 | Reverse transcription and amplification processes and primers therefor |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU5294399A (en) |
GB (1) | GB9817055D0 (en) |
WO (1) | WO2000008208A2 (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7771934B2 (en) | 2000-12-13 | 2010-08-10 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7771946B2 (en) | 2001-03-09 | 2010-08-10 | Nugen Technologies, Inc. | Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US8465950B2 (en) | 2003-04-14 | 2013-06-18 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20020034161A (en) * | 1999-07-19 | 2002-05-08 | 알. 씨. 제닝스 | A method for amplifying low abundance nucleic acid sequences and means for performing said method |
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
TWI237663B (en) * | 2000-12-12 | 2005-08-11 | Masumi Abe | Method of analyzing expression of gene |
EP1275738A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Method for random cDNA synthesis and amplification |
DE60234369D1 (en) * | 2001-09-19 | 2009-12-24 | Alexion Pharma Inc | MANIPULATED MATRICES AND THEIR USE IN SINGLE PRIMER AMPLIFICATION |
US7414111B2 (en) | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
US7176025B2 (en) | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
GB0216026D0 (en) * | 2002-07-10 | 2002-08-21 | Cambridge Biotechnology Ltd | Nucleic acid amplification method |
GB2533882B (en) | 2012-01-26 | 2016-10-12 | Nugen Tech Inc | Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing |
SG11201408478QA (en) | 2012-06-18 | 2015-02-27 | Nugen Technologies Inc | Compositions and methods for negative selection of non-desired nucleic acid sequences |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
WO2014144092A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
JP6525473B2 (en) | 2013-11-13 | 2019-06-05 | ニューゲン テクノロジーズ, インコーポレイテッド | Compositions and methods for identifying replicate sequencing leads |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
CA2957633A1 (en) | 2014-08-06 | 2016-02-11 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991015601A1 (en) * | 1990-04-05 | 1991-10-17 | The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce | Modified rna template-specific polymerase chain reaction |
WO1998008973A1 (en) * | 1996-08-29 | 1998-03-05 | Cancer Research Campaign Technology Limited | Global amplification of nucleic acids |
-
1998
- 1998-08-05 GB GBGB9817055.8A patent/GB9817055D0/en not_active Ceased
-
1999
- 1999-08-05 WO PCT/GB1999/002579 patent/WO2000008208A2/en active Application Filing
- 1999-08-05 AU AU52943/99A patent/AU5294399A/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991015601A1 (en) * | 1990-04-05 | 1991-10-17 | The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce | Modified rna template-specific polymerase chain reaction |
WO1998008973A1 (en) * | 1996-08-29 | 1998-03-05 | Cancer Research Campaign Technology Limited | Global amplification of nucleic acids |
Non-Patent Citations (6)
Title |
---|
CHEUNG AND NELSON: "WHOLE GENOME AMPLIFICATION USING A DEGENERATE OLIGONUCLEOTIDE PRIMER ALLOWS HUNDREDS OF GENOTYPES TO BE PERFORMED ON LESS THAN ONE NANOGRAM OF GENOMIC DNA", PNAS, vol. 93, 1996, pages 14676 - 14679, XP002128316 * |
DIXON ET AL.: "EXPRESSION PROFILING OF SINGLE CELLS USING 3 PRIME END AMPLIFICATION (TPEA) PCR", NUCLEIC ACIDS RESEARCH, vol. 26, no. 19, October 1998 (1998-10-01), pages 4426 - 4431, XP002128317 * |
FROUSSARD: "A RANDOM-PCR METHOD (rPCR) TO CONSTRUCT WHOLE cDNA LIBRARY FROM LOW AMOUNTS OF RNA", NUCLEIC ACIDS RESEARCH, vol. 20, no. 11, 1992, pages 2900, XP002128315 * |
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS,US,ACADEMIC PRESS, SAN DIEGO, vol. 13, 1992, pages 718-725, XP000199116, ISSN: 0888-7543 * |
ZHAO ET AL.: "3'-END cDNA POOL SUITABLE FOR DIFFERENTIAL DISPLAY FROM A SMALL NUMBER OF CELLS", BIOTECHNIQUES, vol. 24, May 1998 (1998-05-01), pages 842 - 852, XP002128314 * |
ZHAO ET AL.: "NEW PRIMER STRATEGY IMPROVES PRECISION OF DIFFERENTIAL DISPLAY", BIOTECHNIQUES, vol. 18, no. 5, 1995, pages 842 - 850, XP002128267 * |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US8334116B2 (en) | 2000-12-13 | 2012-12-18 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7771934B2 (en) | 2000-12-13 | 2010-08-10 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US8071311B2 (en) | 2001-03-09 | 2011-12-06 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US7771946B2 (en) | 2001-03-09 | 2010-08-10 | Nugen Technologies, Inc. | Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences |
US9181582B2 (en) | 2001-03-09 | 2015-11-10 | Nugen Technologies, Inc. | Compositions for amplification of RNA sequences using composite primers |
US8465950B2 (en) | 2003-04-14 | 2013-06-18 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US9175325B2 (en) | 2003-04-14 | 2015-11-03 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8852867B2 (en) | 2005-09-07 | 2014-10-07 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Also Published As
Publication number | Publication date |
---|---|
GB9817055D0 (en) | 1998-09-30 |
AU5294399A (en) | 2000-02-28 |
WO2000008208A2 (en) | 2000-02-17 |
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