WO2000042417A8 - An apparatus and method for the rapid spectral resolution of confocal images - Google Patents

An apparatus and method for the rapid spectral resolution of confocal images

Info

Publication number
WO2000042417A8
WO2000042417A8 PCT/US1999/030863 US9930863W WO0042417A8 WO 2000042417 A8 WO2000042417 A8 WO 2000042417A8 US 9930863 W US9930863 W US 9930863W WO 0042417 A8 WO0042417 A8 WO 0042417A8
Authority
WO
WIPO (PCT)
Prior art keywords
scanning microscope
confocal scanning
sample
detector
microscope apparatus
Prior art date
Application number
PCT/US1999/030863
Other languages
French (fr)
Other versions
WO2000042417A1 (en
Inventor
Michael J Stimson
John D Simon
Original Assignee
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duke filed Critical Univ Duke
Priority to CA002368156A priority Critical patent/CA2368156A1/en
Priority to EP99969290A priority patent/EP1117987A4/en
Priority to AU28455/00A priority patent/AU2845500A/en
Publication of WO2000042417A1 publication Critical patent/WO2000042417A1/en
Publication of WO2000042417A8 publication Critical patent/WO2000042417A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2803—Investigating the spectrum using photoelectric array detector
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

A confocal scanning microscope apparatus and method is used to rapidly acquire spectrally resolved images. The confocal scanning microscope apparatus includes optics used to simultaneously acquire at least two points along a scan pattern on a sample plane of a sample, wherein the points include regions of the sample represented by at least two pixels. A detection arm (418) is placed in the path of the light reflected, scattered, or emitted from the sample plane (414) comprising a spectrometer (428) having a slit for receiving such light and a detector array (432) placed behind the spectrometer. The image corresponding to the at least two points is recorded on the first axis of said detector and spectral resolution thereof is simultaneously recorded on the second axis of the detector. The confocal scanning microscope apparatus and method offers significant improvements to the current techniques for genetic sequencing, as well as significant improvements to traditional confocal scanning microscope applications.
PCT/US1999/030863 1999-01-14 1999-12-23 An apparatus and method for the rapid spectral resolution of confocal images WO2000042417A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002368156A CA2368156A1 (en) 1999-01-14 1999-12-23 An apparatus and method for the rapid spectral resolution of confocal images
EP99969290A EP1117987A4 (en) 1999-01-14 1999-12-23 An apparatus and method for the rapid spectral resolution of confocal images
AU28455/00A AU2845500A (en) 1999-01-14 1999-12-23 An apparatus and method for the rapid spectral resolution of confocal images

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/229,874 1999-01-14
US09/229,874 US6134002A (en) 1999-01-14 1999-01-14 Apparatus and method for the rapid spectral resolution of confocal images

Publications (2)

Publication Number Publication Date
WO2000042417A1 WO2000042417A1 (en) 2000-07-20
WO2000042417A8 true WO2000042417A8 (en) 2001-02-22

Family

ID=22863019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030863 WO2000042417A1 (en) 1999-01-14 1999-12-23 An apparatus and method for the rapid spectral resolution of confocal images

Country Status (5)

Country Link
US (1) US6134002A (en)
EP (1) EP1117987A4 (en)
AU (1) AU2845500A (en)
CA (1) CA2368156A1 (en)
WO (1) WO2000042417A1 (en)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6388788B1 (en) * 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
AU2003204937B2 (en) * 1998-03-16 2004-12-02 Ge Healthcare Bio-Sciences Corp. Confocal microscopy imaging system
JP4270610B2 (en) * 1998-09-24 2009-06-03 オリンパス株式会社 Scanning optical microscope
US7068825B2 (en) * 1999-03-08 2006-06-27 Orametrix, Inc. Scanning system and calibration method for capturing precise three-dimensional information of objects
US6937330B2 (en) 1999-04-23 2005-08-30 Ppd Biomarker Discovery Sciences, Llc Disposable optical cuvette cartridge with low fluorescence material
US6687395B1 (en) * 1999-07-21 2004-02-03 Surromed, Inc. System for microvolume laser scanning cytometry
FR2797495B1 (en) * 1999-08-11 2003-01-31 Dilor SPECTROMETRIC IMAGING APPARATUS
US6329661B1 (en) * 2000-02-29 2001-12-11 The University Of Chicago Biochip scanner device
JP4188538B2 (en) * 2000-04-12 2008-11-26 浜松ホトニクス株式会社 Immunochromatographic test piece measuring device
US6628442B1 (en) * 2000-04-21 2003-09-30 Pixar Animation Studios Method and apparatus for beam deflection using multiple beam scanning galvanometers
DE10029680B4 (en) * 2000-06-23 2016-06-16 Leica Microsystems Cms Gmbh The microscope assemblage
EP1324021B1 (en) * 2000-09-04 2008-01-02 Hamamatsu Photonics K.K. Imaging apparatus
DE10044308A1 (en) * 2000-09-07 2002-03-21 Leica Microsystems Method and device for the detection of fluorescent light in confocal scanning microscopy
DE10046410A1 (en) * 2000-09-18 2002-03-28 Leica Microsystems Optical arrangement for illuminating objects
US6787761B2 (en) 2000-11-27 2004-09-07 Surromed, Inc. Median filter for liquid chromatography-mass spectrometry data
US6826424B1 (en) * 2000-12-19 2004-11-30 Haishan Zeng Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices
US6630680B2 (en) * 2000-12-25 2003-10-07 Fuji Photo Film Co., Ltd. Scanner having confocal optical system, method for producing focus position data of confocal optical system of scanner having confocal optical system and method for producing digital data of scanner having confocal optical system
EP1386139A2 (en) * 2001-05-07 2004-02-04 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Fluorescence fluctuation microscope analytical module or scanning module, method for measurement of fluorescence fluctuation and method and device for adjustment of a fluorescence fluctuation microscope
WO2003014400A1 (en) * 2001-08-08 2003-02-20 Applied Precision, Llc Time-delay integration imaging of biological specimens
US6873915B2 (en) 2001-08-24 2005-03-29 Surromed, Inc. Peak selection in multidimensional data
US20050227231A1 (en) * 2001-10-04 2005-10-13 Dimitri Tcherkassov Device for sequencing nucleic acid molecules
US20030078739A1 (en) * 2001-10-05 2003-04-24 Surromed, Inc. Feature list extraction from data sets such as spectra
DE10150542B4 (en) * 2001-10-12 2007-03-29 Leica Microsystems Cms Gmbh Method for fluorescence microscopy
JP3678192B2 (en) * 2001-11-21 2005-08-03 横河電機株式会社 Measuring device
DE20216583U1 (en) * 2001-12-20 2003-01-23 Leica Microsystems Microscope and flow cytometer
US20030148391A1 (en) * 2002-01-24 2003-08-07 Salafsky Joshua S. Method using a nonlinear optical technique for detection of interactions involving a conformational change
WO2003095978A2 (en) 2002-05-09 2003-11-20 Surromed, Inc. Methods for time-alignment of liquid chromatography-mass spectrometry data
DE10221564A1 (en) * 2002-05-15 2003-11-27 Evotec Ag Photoluminescence analyzer for chemical and biological sample screening uses a high intensity homogeneous line image as illumination source, and has multiple sensor scanning of the image
JP4074136B2 (en) * 2002-05-29 2008-04-09 浜松ホトニクス株式会社 Fluorescence lifetime distribution image measuring apparatus and measuring method thereof
DE10303404A1 (en) * 2003-01-27 2004-08-05 Carl Zeiss Jena Gmbh Method for the detection of fluorescent light
US20040263959A1 (en) * 2003-06-30 2004-12-30 Dixon Arthur E. Scanning beam optical imaging system for macroscopic imaging of an object
US7075100B2 (en) * 2003-09-22 2006-07-11 Honeywell International Inc. Confocal scanner system and method
US7589891B2 (en) * 2003-11-26 2009-09-15 Olympus Corporation Laser scanning type fluorescent microscope
US7248360B2 (en) * 2004-04-02 2007-07-24 Ppd Biomarker Discovery Sciences, Llc Polychronic laser scanning system and method of use
US20050225849A1 (en) * 2004-04-05 2005-10-13 Fujifilm Electronic Imaging Ltd. Slit confocal microscope and method
JP4445796B2 (en) * 2004-05-17 2010-04-07 オリンパス株式会社 microscope
US7170675B2 (en) * 2004-05-19 2007-01-30 Celloptic, Inc. Method and system for wide-field multi-photon microscopy having a confocal excitation plane
DE102004034968A1 (en) * 2004-07-16 2006-02-09 Carl Zeiss Jena Gmbh Scanning microscope with linear scan
DE102004034983A1 (en) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Light scanning microscope
DE102004034970A1 (en) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Scanning microscope and use
DE102004034977A1 (en) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Scanning microscope and use
US7719729B2 (en) * 2004-09-30 2010-05-18 Pixar Anamorphic recording method and apparatus
US20060238756A1 (en) * 2005-02-10 2006-10-26 Bearman Gregory H Fast spectral confocal imager
WO2007023946A1 (en) * 2005-08-26 2007-03-01 Brother Kogyo Kabushiki Kaisha Light scan device, image display device, and retina scan type image display device
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
JP4887989B2 (en) * 2005-12-02 2012-02-29 ナノフォトン株式会社 Optical microscope and spectrum measuring method
US20070171408A1 (en) * 2006-01-04 2007-07-26 Xinghua Wang Method and apparatus for dense spectrum unmixing and image reconstruction of a sample
US7973927B2 (en) * 2006-09-29 2011-07-05 Uwm Research Foundation, Inc. Two-photon microscope with spectral resolution
US7813013B2 (en) * 2006-11-21 2010-10-12 Illumina, Inc. Hexagonal site line scanning method and system
US7791013B2 (en) * 2006-11-21 2010-09-07 Illumina, Inc. Biological microarray line scanning method and system
US7817275B2 (en) * 2007-03-07 2010-10-19 University Of Delaware Scanning optical microscope with long working distance objective
EP2225598A1 (en) * 2007-12-21 2010-09-08 Koninklijke Philips Electronics N.V. Scanning microscope and method of imaging a sample.
US8696695B2 (en) 2009-04-28 2014-04-15 Avinger, Inc. Guidewire positioning catheter
US8062316B2 (en) 2008-04-23 2011-11-22 Avinger, Inc. Catheter system and method for boring through blocked vascular passages
US9125562B2 (en) 2009-07-01 2015-09-08 Avinger, Inc. Catheter-based off-axis optical coherence tomography imaging system
US9498600B2 (en) 2009-07-01 2016-11-22 Avinger, Inc. Atherectomy catheter with laterally-displaceable tip
US7954953B2 (en) * 2008-07-30 2011-06-07 Microvision, Inc. Scanned beam overlay projection
US9182406B2 (en) 2008-08-04 2015-11-10 Biodesy, Inc. Nonlinear optical detection of molecules comprising an unnatural amino acid possessing a hyperpolarizability
WO2010042576A1 (en) * 2008-10-09 2010-04-15 Ge Healthcare Bio-Sciences Corp. A system and method for adjusting a beam expander in an imaging system
TWI388879B (en) * 2008-11-13 2013-03-11 Univ Nat Taiwan Reflective optical scanning device with minimal aberration
AU2010253912B2 (en) 2009-05-28 2015-03-05 Avinger, Inc. Optical Coherence Tomography for biological imaging
DE102009057304A1 (en) * 2009-12-07 2011-06-09 Leica Microsystems Cms Gmbh Apparatus for examining a sample comprising a microscope
EP2509498B1 (en) 2009-12-08 2020-09-16 Avinger, Inc. Devices for predicting and preventing restenosis
US9345510B2 (en) 2010-07-01 2016-05-24 Avinger, Inc. Atherectomy catheters with longitudinally displaceable drive shafts
US10548478B2 (en) 2010-07-01 2020-02-04 Avinger, Inc. Balloon atherectomy catheters with imaging
US11382653B2 (en) 2010-07-01 2022-07-12 Avinger, Inc. Atherectomy catheter
WO2012129347A1 (en) 2011-03-21 2012-09-27 Biodesy, Llc Classification of kinase inhibitors using nonlinear optical techniques
JP6205344B2 (en) 2011-03-28 2017-09-27 アビンガー・インコーポレイテッドＡｖｉｎｇｅｒ， Ｉｎｃ． Occlusion crossing device, imaging device and atherectomy device
US9949754B2 (en) 2011-03-28 2018-04-24 Avinger, Inc. Occlusion-crossing devices
US20120257037A1 (en) 2011-04-07 2012-10-11 Valerica Raicu High speed microscope with two-stage scanning for detection of rarities in samples
CA2835713C (en) * 2011-05-13 2023-04-04 Fibics Incorporated Microscopy imaging method and system
US20130015370A1 (en) * 2011-07-15 2013-01-17 Huron Technologies International In Confocal fluorescence slide scanner with parallel detection
JP6356604B2 (en) 2011-10-17 2018-07-11 アビンガー・インコーポレイテッドＡｖｉｎｇｅｒ， Ｉｎｃ． Atherotomy catheters and non-contact actuation mechanisms for catheters
US9345406B2 (en) 2011-11-11 2016-05-24 Avinger, Inc. Occlusion-crossing devices, atherectomy devices, and imaging
WO2013172974A1 (en) 2012-05-14 2013-11-21 Avinger, Inc. Atherectomy catheter drive assemblies
US11406412B2 (en) 2012-05-14 2022-08-09 Avinger, Inc. Atherectomy catheters with imaging
WO2013172972A1 (en) 2012-05-14 2013-11-21 Avinger, Inc. Optical coherence tomography with graded index fiber for biological imaging
US10335173B2 (en) 2012-09-06 2019-07-02 Avinger, Inc. Re-entry stylet for catheter
US11284916B2 (en) 2012-09-06 2022-03-29 Avinger, Inc. Atherectomy catheters and occlusion crossing devices
US9498247B2 (en) 2014-02-06 2016-11-22 Avinger, Inc. Atherectomy catheters and occlusion crossing devices
US9854979B2 (en) 2013-03-15 2018-01-02 Avinger, Inc. Chronic total occlusion crossing devices with imaging
WO2014142954A1 (en) 2013-03-15 2014-09-18 Avinger, Inc. Tissue collection device for catheter
JP6291025B2 (en) 2013-03-15 2018-03-14 アビンガー・インコーポレイテッドＡｖｉｎｇｅｒ， Ｉｎｃ． Optical pressure sensor assembly
EP3019096B1 (en) 2013-07-08 2023-07-05 Avinger, Inc. System for identification of elastic lamina to guide interventional therapy
EP3068900B1 (en) * 2013-11-17 2020-01-08 Quantum-Si Incorporated Active-source-pixel, integrated device for rapid analysis of biological and chemical speciments
CA2938972A1 (en) 2014-02-06 2015-08-13 Avinger, Inc. Atherectomy catheters and occlusion crossing devices
WO2016007652A1 (en) 2014-07-08 2016-01-14 Avinger, Inc. High speed chronic total occlusion crossing devices
GB2528864A (en) * 2014-07-31 2016-02-10 Technion Res & Dev Foundation Spectral imaging using single-axis spectrally dispersed illumination
EP3237906B8 (en) 2014-12-23 2020-10-28 Bluelight Therapeutics, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
US10568520B2 (en) 2015-07-13 2020-02-25 Avinger, Inc. Micro-molded anamorphic reflector lens for image guided therapeutic/diagnostic catheters
DE102015116598A1 (en) 2015-09-30 2017-03-30 Carl Zeiss Microscopy Gmbh Method and microscope for high-resolution imaging by means of SIM
US10295811B2 (en) * 2015-11-01 2019-05-21 Howard Hughes Medical Institute Large field of view, high resolution microscope
US11278248B2 (en) 2016-01-25 2022-03-22 Avinger, Inc. OCT imaging catheter with lag correction
JP6959255B2 (en) 2016-04-01 2021-11-02 アビンガー・インコーポレイテッドＡｖｉｎｇｅｒ， Ｉｎｃ． Catheter device for porridge resection
WO2017209079A1 (en) 2016-05-30 2017-12-07 株式会社ニコン Observing device and observing method
JP2019518543A (en) 2016-06-03 2019-07-04 アビンガー・インコーポレイテッドＡｖｉｎｇｅｒ， Ｉｎｃ． Catheter device having a removable distal end
US11224459B2 (en) 2016-06-30 2022-01-18 Avinger, Inc. Atherectomy catheter with shapeable distal tip
CN108152200A (en) * 2016-12-06 2018-06-12 北京世纪桑尼科技有限公司 A kind of co-focusing imaging device and method for spectrum analysis
DE102018009056A1 (en) * 2018-11-12 2020-05-14 Carl Zeiss Microscopy Gmbh Accelerated methods and devices for three-dimensional microscopy with structured illumination
CN109965841A (en) * 2019-04-23 2019-07-05 广东唯仁医疗科技有限公司 A kind of flexibility analysis device and method of intraocular lens implantation
US11793400B2 (en) 2019-10-18 2023-10-24 Avinger, Inc. Occlusion-crossing devices
RU2723890C1 (en) * 2019-11-07 2020-06-18 федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный университет" (ФГБОУ ВО "ВГУ") Multichannel confocal image spectrum analyzer
US11803043B2 (en) * 2019-12-17 2023-10-31 University Of Washington System and apparatus for dynamically shaped focal surface with a scanning microscope

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9014263D0 (en) * 1990-06-27 1990-08-15 Dixon Arthur E Apparatus and method for spatially- and spectrally- resolvedmeasurements
US5991028A (en) * 1991-02-22 1999-11-23 Applied Spectral Imaging Ltd. Spectral bio-imaging methods for cell classification
US5822061A (en) * 1992-01-20 1998-10-13 Dilor Spectrometry apparatus
IL108497A0 (en) * 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
JP3343276B2 (en) * 1993-04-15 2002-11-11 興和株式会社 Laser scanning optical microscope
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
US5381224A (en) * 1993-08-30 1995-01-10 A. E. Dixon Scanning laser imaging system
US5532873A (en) * 1993-09-08 1996-07-02 Dixon; Arthur E. Scanning beam laser microscope with wide range of magnification
US5886784A (en) * 1993-09-08 1999-03-23 Leica Lasertechink Gmbh Device for the selection and detection of at least two spectral regions in a beam of light
US5903688A (en) * 1994-08-25 1999-05-11 Leica Lasertechnik Gmbh Device for feeding a UV laser into a confocal laser scanning microscope
JP3816632B2 (en) * 1997-05-14 2006-08-30 オリンパス株式会社 Scanning microscope

Also Published As

Publication number Publication date
US6134002A (en) 2000-10-17
WO2000042417A1 (en) 2000-07-20
AU2845500A (en) 2000-08-01
EP1117987A4 (en) 2004-08-11
EP1117987A2 (en) 2001-07-25
CA2368156A1 (en) 2000-07-20

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