WO2000052155A3 - Recombination of insertion modified nucleic acids - Google Patents

Recombination of insertion modified nucleic acids Download PDF

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Publication number
WO2000052155A3
WO2000052155A3 PCT/US2000/005573 US0005573W WO0052155A3 WO 2000052155 A3 WO2000052155 A3 WO 2000052155A3 US 0005573 W US0005573 W US 0005573W WO 0052155 A3 WO0052155 A3 WO 0052155A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombination
nucleic acids
modified nucleic
insertion modified
tuning
Prior art date
Application number
PCT/US2000/005573
Other languages
French (fr)
Other versions
WO2000052155A2 (en
Inventor
Phillip A Patten
Volker Heinrichs
Willem P C Stemmer
Original Assignee
Maxygen Inc
Phillip A Patten
Volker Heinrichs
Willem P C Stemmer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Phillip A Patten, Volker Heinrichs, Willem P C Stemmer filed Critical Maxygen Inc
Priority to AU37195/00A priority Critical patent/AU3719500A/en
Priority to EP00916027A priority patent/EP1165775A2/en
Priority to CA002362737A priority patent/CA2362737A1/en
Publication of WO2000052155A2 publication Critical patent/WO2000052155A2/en
Priority to US09/710,686 priority patent/US6531316B1/en
Publication of WO2000052155A3 publication Critical patent/WO2000052155A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.
PCT/US2000/005573 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids WO2000052155A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU37195/00A AU3719500A (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids
EP00916027A EP1165775A2 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids
CA002362737A CA2362737A1 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids
US09/710,686 US6531316B1 (en) 1999-03-05 2000-11-09 Encryption of traits using split gene sequences and engineered genetic elements

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US12294399P 1999-03-05 1999-03-05
US60/122,943 1999-03-05
US14229999P 1999-07-02 1999-07-02
US60/142,299 1999-07-02
US16461899P 1999-11-10 1999-11-10
US16461799P 1999-11-10 1999-11-10
US60/164,618 1999-11-10
US60/164,617 1999-11-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US09/517,933 Continuation-In-Part US6365377B1 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005448 Continuation-In-Part WO2000052146A2 (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences

Publications (2)

Publication Number Publication Date
WO2000052155A2 WO2000052155A2 (en) 2000-09-08
WO2000052155A3 true WO2000052155A3 (en) 2001-07-26

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2000/005573 WO2000052155A2 (en) 1999-03-05 2000-03-03 Recombination of insertion modified nucleic acids
PCT/US2000/005448 WO2000052146A2 (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005448 WO2000052146A2 (en) 1999-03-05 2000-03-03 Encryption of traits using split gene sequences

Country Status (5)

Country Link
US (4) US6365377B1 (en)
EP (2) EP1165757A1 (en)
AU (2) AU3719500A (en)
CA (2) CA2364997A1 (en)
WO (2) WO2000052155A2 (en)

Families Citing this family (149)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US7670823B1 (en) * 1999-03-02 2010-03-02 Life Technologies Corp. Compositions for use in recombinational cloning of nucleic acids
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) * 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
CN101125873A (en) * 1997-10-24 2008-02-20 茵维特罗根公司 Recombinational cloning using nucleic acids having recombination sites
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CA2307016A1 (en) * 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US20030092023A1 (en) * 1998-08-12 2003-05-15 Daniel Dupret Method of shuffling polynucleotides using templates
US6991922B2 (en) * 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
US20040191772A1 (en) * 1998-08-12 2004-09-30 Dupret Daniel Marc Method of shuffling polynucleotides using templates
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
CA2374497A1 (en) * 1999-05-24 2000-11-30 New England Biolabs, Inc. Method for generating split, non-transferable genes that are able to express an active protein product
EP1978098A3 (en) 1999-12-10 2009-02-18 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
JP2003519495A (en) * 2000-01-11 2003-06-24 マキシジェン， インコーポレイテッド Integrated systems and methods for diversity generation and screening
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
WO2002086144A2 (en) * 2001-04-19 2002-10-31 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
JP2004532636A (en) * 2001-05-21 2004-10-28 インヴィトロジェン コーポレーション Compositions and methods for use in isolating nucleic acid molecules
CN101993907B (en) 2002-01-08 2018-12-28 迈克尔·R·拉尔布 Expressing CIVPS or the genetically modified plants of intein modified proteins and preparation method thereof
US20030167533A1 (en) * 2002-02-04 2003-09-04 Yadav Narendra S. Intein-mediated protein splicing
US20040132101A1 (en) 2002-09-27 2004-07-08 Xencor Optimized Fc variants and methods for their generation
US20040214263A1 (en) * 2002-03-20 2004-10-28 Mitchell Lloyd G. Spliceosome mediated RNA trans-splicing
US7238854B2 (en) * 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
US20060073481A1 (en) * 2002-04-23 2006-04-06 The Molecular Sciences Institute Formation and use of site specific nucleic acid coupled binding polypeptides
US20030198973A1 (en) * 2002-04-23 2003-10-23 The Molecular Sciences Institute, Inc. Chimeric fusion molecule for analyte detection and quantitation
MXPA04010800A (en) * 2002-04-29 2005-03-07 Icon Genetics Ag Process of producing environmentally safe transgenic organisms.
WO2003097834A2 (en) * 2002-05-17 2003-11-27 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
EP1509610B1 (en) 2002-05-31 2012-05-23 Bayer CropScience N.V. Transgenic plants with controlled distribution of a trait to progeny
WO2004009768A2 (en) * 2002-07-18 2004-01-29 Invitrogen Corporation Viral vectors containing recombination sites
AU2003304275A1 (en) 2002-08-06 2005-01-21 Pioneer Hi-Bred International, Inc. Ap1 amine oxidase variants
WO2004029207A2 (en) 2002-09-27 2004-04-08 Xencor Inc. Optimized fc variants and methods for their generation
DE10254165A1 (en) 2002-11-20 2004-06-03 Icon Genetics Ag Method for controlling a cellular process in a multicellular organism
DE10254167A1 (en) * 2002-11-20 2004-06-09 Icon Genetics Ag Process for the control of cellular processes in plants
US20040177400A1 (en) * 2003-02-28 2004-09-09 Wahlroos Tony Mikaer Methods and constructs for increasing the content of selected amino acids in seeds
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
US20060134105A1 (en) * 2004-10-21 2006-06-22 Xencor, Inc. IgG immunoglobulin variants with optimized effector function
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
US8399618B2 (en) 2004-10-21 2013-03-19 Xencor, Inc. Immunoglobulin insertions, deletions, and substitutions
ATE469984T1 (en) 2003-12-01 2010-06-15 Life Technologies Corp NUCLEIC ACID MOLECULES CONTAINING RECOMBINATION SITE AND METHOD FOR USE THEREOF
EP1697741A4 (en) * 2003-12-04 2008-02-13 Xencor Inc Methods of generating variant proteins with increased host string content and compositions thereof
KR101203606B1 (en) * 2003-12-16 2012-11-23 도시카즈 나카무라 Glycosylation-deficient hepatocyte growth factor
US8729341B2 (en) * 2004-02-23 2014-05-20 University Of Chicago Plants modified with mini-chromosomes
US7276585B2 (en) 2004-03-24 2007-10-02 Xencor, Inc. Immunoglobulin variants outside the Fc region
KR101027427B1 (en) 2004-11-12 2011-04-11 젠코어 인코포레이티드 Fc VARIANTS WITH INCREASED BINDING TO FcRn
JP4937138B2 (en) 2005-01-05 2012-05-23 エフ−シュタール・ビオテヒノロギシェ・フォルシュングス−ウント・エントヴィックルングスゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Synthetic immunoglobulin domains with binding properties designed in a region of the molecule that is different from the complementarity-determining region
MX2007016032A (en) 2005-06-21 2008-03-10 Xoma Technology Ltd Il-1 beta binding antibodies and fragments thereof.
JP2009508470A (en) 2005-07-21 2009-03-05 アボット・ラボラトリーズ Multiple gene expression including SORF constructs and polyprotein, proprotein and proteolytic methods
CA2621874C (en) * 2005-09-08 2014-12-16 Chromatin Inc. Plants modified with mini-chromosomes
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
AT503889B1 (en) 2006-07-05 2011-12-15 Star Biotechnologische Forschungs Und Entwicklungsges M B H F MULTIVALENT IMMUNE LOBULINE
WO2008112972A2 (en) * 2007-03-15 2008-09-18 Chromatin, Inc. Centromere sequences and minichromosomes
JP5602625B2 (en) 2007-06-26 2014-10-08 エフ−スター ビオテヒノロギッシェ フォルシュングス− ウント エントヴィッケルングスゲゼルシャフト ミット ベシュレンクテル ハフツング Binding substance display
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
EP2113255A1 (en) 2008-05-02 2009-11-04 f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H. Cytotoxic immunoglobulin
EP2456894A4 (en) 2009-07-23 2013-09-25 Chromatin Inc Sorghum centromere sequences and minichromosomes
EP2281544A1 (en) * 2009-07-27 2011-02-09 The Procter & Gamble Company Oral care compositions which comprise stannous, potassium and monoflurophosphate
EP2281543A1 (en) * 2009-07-27 2011-02-09 The Procter & Gamble Company Oral care compositions which comprise stannous and potassium salts
RU2012122240A (en) * 2009-10-30 2013-12-10 Эбботт Лэборетриз SORF DESIGNS AND EXPRESSION OF SEVERAL GENES
US9464333B2 (en) 2009-11-06 2016-10-11 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US10407742B2 (en) 2009-11-06 2019-09-10 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US8420387B2 (en) 2009-11-06 2013-04-16 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
CN103080306B (en) 2010-08-20 2015-02-18 科德克希思公司 Use of glycoside hydrolase 61 family proteins in processing of cellulose
US9322027B2 (en) 2010-08-20 2016-04-26 Shell Oil Company Expression constructs comprising fungal promoters
ES2610923T3 (en) 2011-03-07 2017-05-04 Agrivida, Inc. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degradation enzymes
EP2691517A4 (en) 2011-03-30 2015-04-22 Codexis Inc Pentose fermentation by a recombinant microorganism
BR112013033614A2 (en) 2011-06-30 2018-05-29 Codexis Inc pentose fermentation by recombinant microorganism
DK2748317T3 (en) 2011-08-22 2017-07-17 Codexis Inc GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
EP2760997A4 (en) 2011-09-30 2015-02-11 Codexis Inc Fungal proteases
WO2013096244A1 (en) 2011-12-20 2013-06-27 Codexis, Inc. Endoglucanase 1b (eg1b) variants
DK2859099T3 (en) 2012-06-11 2019-07-29 Codexis Inc MUSHROOMS-BETA-xylosidase VERSIONS
AU2013326968B2 (en) * 2012-10-03 2019-08-08 Agrivida, Inc. Multiprotein expression cassettes
EP2922953A4 (en) 2012-11-20 2016-11-09 Shell Int Research Pentose fermentation by a recombinant microorganism
CN105473605A (en) 2013-03-15 2016-04-06 先锋国际良种公司 Phi-4 polypeptides and methods for their use
CA2909719C (en) 2013-04-18 2022-09-27 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
EP3032942B1 (en) 2013-08-16 2020-03-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US10667524B2 (en) 2013-09-13 2020-06-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2015048572A1 (en) 2013-09-27 2015-04-02 Codexis, Inc. Automated screening of enzyme variants
WO2015073555A1 (en) 2013-11-13 2015-05-21 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
BR112016018287A2 (en) 2014-02-07 2017-10-10 Du Pont insecticide proteins and methods for their use
CN114763376A (en) 2014-02-07 2022-07-19 先锋国际良种公司 Insecticidal proteins and methods of use thereof
US9605252B2 (en) 2014-04-16 2017-03-28 Codexis, Inc. Engineered tyrosine ammonia lyase
DK3167052T3 (en) 2014-07-09 2020-02-10 Codexis Inc P450-BM3 VARIATIONS WITH IMPROVED ACTIVITY
CN113372421A (en) 2014-10-16 2021-09-10 先锋国际良种公司 Insecticidal proteins and methods of use thereof
WO2016069774A1 (en) * 2014-10-28 2016-05-06 Agrivida, Inc. Methods and compositions for stabilizing trans-splicing intein modified proteases
DK3224354T3 (en) 2014-11-25 2021-03-01 Codexis Inc MANIPULATED IMINREDUCTASES AND PROCEDURES FOR THE REDUCTIVE AMINATION OF KETONE AND AMIN COMPOUNDS
HUE062016T2 (en) 2014-12-22 2023-09-28 Codexis Inc Human alpha-galactosidase variants
US10696953B2 (en) 2015-02-10 2020-06-30 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
MX2017014213A (en) 2015-05-07 2018-03-28 Codexis Inc Penicillin-g acylases.
WO2016186986A1 (en) 2015-05-19 2016-11-24 Pioneer Hi Bred International Inc Insecticidal proteins and methods for their use
CN108271374B (en) 2015-07-07 2021-09-21 科德克希思公司 Novel P450-BM3 variants with improved activity
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EA201892293A1 (en) 2016-05-04 2019-04-30 Пайонир Хай-Бред Интернэшнл, Инк. INSECTICIDAL PROTEINS AND METHODS OF THEIR APPLICATION
US10724025B2 (en) 2016-05-05 2020-07-28 Codexis, Inc. Penicillin-G acylases
WO2017213758A1 (en) 2016-06-09 2017-12-14 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
BR112018075932A2 (en) 2016-06-15 2019-10-01 Codexis Inc unnaturally occurring beta-glycosidase variant, recombinant polynucleotide, vector, host cell, methods for producing at least one unnaturally occurring beta-glucosidase variant for conversion of a glycosyl group-acceptor substrate to a beta-glycosylated product and for transglycosylation of a substrate, and, composition.
EP3478052B1 (en) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
EP3515926B1 (en) 2016-08-26 2023-10-25 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
CN110088123B (en) 2016-12-14 2023-10-20 先锋国际良种公司 Insecticidal proteins and methods of use thereof
CA3046226A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
KR20190102063A (en) 2017-01-05 2019-09-02 코덱시스, 인코포레이티드 Penicillin Ｇ acylase
TW202237834A (en) 2017-02-03 2022-10-01 美商克迪科思股份有限公司 Engineered glycosyltransferases and steviol glycoside glucosylation methods
US20190390219A1 (en) 2017-02-08 2019-12-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
JP2020505942A (en) 2017-02-13 2020-02-27 コデクシス， インコーポレイテッド Phenylalanine ammonia lyase polypeptide
US11021729B2 (en) 2017-04-27 2021-06-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
CA3062550A1 (en) 2017-05-08 2018-11-15 Codexis, Inc. Engineered ligase variants
US11555203B2 (en) 2017-05-11 2023-01-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3638688A4 (en) 2017-06-14 2021-07-07 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
CN111051506B (en) 2017-06-27 2023-11-24 科德克希思公司 Penicillin G acylase
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MX2020006222A (en) 2017-12-13 2020-08-31 Codexis Inc Carboxyesterase polypeptides for amide coupling.
WO2019241132A1 (en) 2018-06-12 2019-12-19 Codexis, Inc. Engineered tyrosine ammonia lyase
MX2021000328A (en) 2018-07-09 2021-03-25 Codexis Inc Engineered galactose oxidase variant enzymes.
MX2021000322A (en) 2018-07-09 2021-03-25 Codexis Inc Engineered phosphopentomutase variant enzymes.
SG11202012111UA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered purine nucleoside phosphorylase variant enzymes
CN112673091A (en) 2018-07-09 2021-04-16 科德克希思公司 Engineered pantothenate kinase variant enzymes
SG11202012107RA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered deoxyribose-phosphate aldolases
CN112672989A (en) 2018-07-12 2021-04-16 科德克希思公司 Engineered phenylalanine ammonia lyase polypeptides
KR20210040408A (en) 2018-07-30 2021-04-13 코덱시스, 인코포레이티드 Engineered glycosyltransferase and steviol glycoside glucosylation method
WO2020046701A1 (en) 2018-08-29 2020-03-05 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2020092216A1 (en) 2018-10-29 2020-05-07 Codexis, Inc. Engineered dna polymerase variants
AU2019397401A1 (en) 2018-12-14 2021-06-17 Codexis, Inc. Engineered tyrosine ammonia lyase
CA3123598A1 (en) 2018-12-20 2020-06-25 Codexis, Inc. Human alpha-galactosidase variants
CN115427557A (en) 2020-04-10 2022-12-02 科德克希思公司 Engineered transaminase polypeptides
BR112022027035A2 (en) 2020-07-14 2023-04-11 Pioneer Hi Bred Int INSECTICIDAL PROTEINS AND METHODS FOR THE USE OF THEM
EP4204552A1 (en) 2020-08-28 2023-07-05 Codexis, Inc. Engineered amylase variants
EP4262804A2 (en) 2020-12-18 2023-10-25 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
CA3214975A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
CA3214973A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered guanylate kinase variant enzymes
IL305928A (en) 2021-04-02 2023-11-01 Codexis Inc Engineered acetate kinase variant enzymes
WO2022212824A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered adenylate kinase variant enzymes
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015388A1 (en) * 1993-12-03 1995-06-08 Medical Research Council Recombinant binding proteins and peptides
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1997001642A1 (en) * 1995-06-28 1997-01-16 New England Biolabs, Inc. Modified proteins and methods of their production
WO1998040519A1 (en) * 1997-03-11 1998-09-17 Trustees Of Boston University Intron-mediated recombinant techniques and reagents

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62502586A (en) 1985-03-18 1987-10-08 ジ−ン　ラブズ，インコ−ポレイテツド Hybrid gene cassette vector
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
DE3588239T3 (en) 1985-03-30 2007-03-08 Kauffman, Stuart A., Santa Fe A method for obtaining DNA, RNA, peptides, polypeptides or proteins by DMS recombinant methods
US5866363A (en) 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US5824469A (en) 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
DD282028A5 (en) 1989-02-16 1990-08-29 Akad Wissenschaften Ddr PROCESS FOR PREPARING THERMOSTABILES, HYBRIDEN BACILLUS BETA-1,3-1,4-GLUCANASE
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
EP0478627A4 (en) 1989-05-16 1992-08-19 William D. Huse Co-expression of heteromeric receptors
US5252745A (en) 1989-08-25 1993-10-12 Rohm And Haas Company Preparation and use of iodopropargyl esters of α-amino acid derivatives as antimicrobial agents
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
JP3306063B2 (en) 1990-08-24 2002-07-24 イグジス， インコーポレイテッド Method for synthesizing oligonucleotides having random codons
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
IL99553A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
ATE177782T1 (en) 1990-12-20 1999-04-15 Ixsys Inc OPTIMIZATION OF BINDING PROTEINS
NZ241310A (en) 1991-01-17 1995-03-28 Gen Hospital Corp Trans-splicing ribozymes
NZ241311A (en) * 1991-01-17 1995-03-28 Gen Hospital Corp Rna sequence having trans-splicing activity, plant strains
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
ATE178650T1 (en) 1992-06-05 1999-04-15 Scripps Research Inst D-ENZYME COMPOSITIONS AND METHODS OF USE THEREOF
EP0675953B1 (en) 1992-11-10 2009-01-28 Applied Molecular Evolution, Inc. Soluble peptides having constrained, secondary conformation in solution and method of making same
US5837242A (en) 1992-12-04 1998-11-17 Medical Research Council Multivalent and multispecific binding proteins, their manufacture and use
EP0602899B1 (en) 1992-12-09 2004-10-20 New England Biolabs, Inc. Modified proteins containing controllable intervening sequences and methods of their production
US5834247A (en) * 1992-12-09 1998-11-10 New England Biolabs, Inc. Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised by a modified protein
US5496714A (en) 1992-12-09 1996-03-05 New England Biolabs, Inc. Modification of protein by use of a controllable interveining protein sequence
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US5667969A (en) 1993-11-12 1997-09-16 University Research Corporation Alteration of sequence of a deleterious target molecule by ribozyme catalyzed trans-splicing
JP3082549B2 (en) 1993-12-27 2000-08-28 株式会社村田製作所 Method for producing ceramic green sheet with supporting film
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5891993A (en) 1994-08-11 1999-04-06 The Scripps Research Institute Template assembled synthetic protein
US5514588A (en) 1994-12-13 1996-05-07 Exxon Research And Engineering Company Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water
US5872241A (en) 1995-01-25 1999-02-16 The Trustees Of Columbia University In The City Of New York Multiple component RNA catalysts and uses thereof
AU723619B2 (en) 1995-04-24 2000-08-31 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
CA2218620C (en) 1995-05-04 2007-02-27 Stephen B. H. Kent Synthesis of proteins by native chemical ligation
US5776744A (en) 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6168919B1 (en) 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
DE69620766T2 (en) 1995-08-11 2004-11-18 Novozymes A/S METHOD FOR THE PRODUCTION OF POLYPEPTIDE COMBS
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5962283A (en) 1995-12-07 1999-10-05 Diversa Corporation Transminases and amnotransferases
US5814473A (en) 1996-02-09 1998-09-29 Diversa Corporation Transaminases and aminotransferases
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
DE69636937T3 (en) 1995-12-15 2011-01-05 Virxsys Corp. THERAPEUTIC MOLECULES OBTAINED BY TRANS-CLEARANCE
EP0873398B1 (en) 1996-01-10 2005-04-20 Novozymes A/S A method for in vivo production of a mutant library in cells
US5942430A (en) 1996-02-16 1999-08-24 Diversa Corporation Esterases
US5958751A (en) 1996-03-08 1999-09-28 Diversa Corporation α-galactosidase
WO1997033957A1 (en) 1996-03-15 1997-09-18 Amway Corporation Powder detergent composition having improved solubility
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5844602A (en) 1996-05-07 1998-12-01 Recon/Optical, Inc. Electro-optical imaging array and camera system with pitch rate image motion compensation which can be used in an airplane in a dive bomb maneuver
US5789228A (en) 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
US5877001A (en) 1996-06-17 1999-03-02 Diverso Corporation Amidase
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
WO1997048416A1 (en) 1996-06-19 1997-12-24 Recombinant Biocatalysis, Inc. Thermostable phosphatases
US5939300A (en) 1996-07-03 1999-08-17 Diversa Corporation Catalases
US5795731A (en) 1996-08-26 1998-08-18 Health Research Incorporated Inteins as antimicrobial targets: genetic screens for intein function
US5862514A (en) 1996-12-06 1999-01-19 Ixsys, Inc. Method and means for synthesis-based simulation of chemicals having biological functions
US5981182A (en) 1997-03-13 1999-11-09 Albert Einstein College Of Medicine Of Yeshiva University Vector constructs for the selection and identification of open reading frames
US5948666A (en) 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US5876997A (en) 1997-08-13 1999-03-02 Diversa Corporation Phytase
US6087341A (en) 1998-02-12 2000-07-11 The Board Of Trustees Of The Leland Standford Junior University Introduction of nucleic acid into skin cells by topical application
AU6258399A (en) * 1998-09-21 2000-04-10 Trustees Of Boston University Intron-mediated recombinant techniques and reagents

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015388A1 (en) * 1993-12-03 1995-06-08 Medical Research Council Recombinant binding proteins and peptides
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1997001642A1 (en) * 1995-06-28 1997-01-16 New England Biolabs, Inc. Modified proteins and methods of their production
WO1998040519A1 (en) * 1997-03-11 1998-09-17 Trustees Of Boston University Intron-mediated recombinant techniques and reagents

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARLO T. ET AL.: "An intron splicing enhancer containing a G-rich repeat facilitates inclusion of a vertebrate micro-exon", RNA, vol. 2, 1996, CAMBRIDGE US, pages 342 - 353, XP000926038 *
HOLFORD M ET AL: "Adding 'splice' to protein engineering", STRUCTURE PHILADELPHIA, PA, US, vol. 6, 15 August 1998 (1998-08-15), pages 951 - 956, XP000864581, ISSN: 0969-2126 *
STEMMER W.P.C.: "DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 91, October 1994 (1994-10-01), WASHINGTON., US, pages 10747 - 10751, XP002917366, ISSN: 0027-8424 *

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