WO2000066028A1 - Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm - Google Patents
Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm Download PDFInfo
- Publication number
- WO2000066028A1 WO2000066028A1 PCT/KR2000/000396 KR0000396W WO0066028A1 WO 2000066028 A1 WO2000066028 A1 WO 2000066028A1 KR 0000396 W KR0000396 W KR 0000396W WO 0066028 A1 WO0066028 A1 WO 0066028A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sperm
- dog
- freezing
- composition
- mixture
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
Definitions
- the present invention relates to compositions for freezing dog sperm, a method of freezing the dog sperm utilizing the compositions and an artificial insemination method employing the frozen dog sperm. More particularly, the present invention relates to compositions for freezing dog sperm in which the number of spermatozoa living in the sperm and the shape and viability thereof are retained when the sperm is thawed within a predetermined time after it is frozen, a method of freezing the dog sperm utilizing the compositions, and an artificial insemination method involving thawing the frozen dog sperm at a suitable place and time and inserting the same into the vagina of the female dog.
- Background Art
- diluent fluids applicable to pigs, horses and cattle have been developed and put into practice.
- development of diluent fluids applicable to dog sperm has not yet matured.
- a method of preserving animal sperm for a long time without loss of freshness would allow superior sperm to be used to fertilize female animals as necessary, which would be a great contribution to the development of livestock and the pet industry.
- a freezing method is most suitable for preserving sperm for a long time.
- composition for freezing dog sperm comprising, based on 100 ml of distilled water, 0.25 to 4.5 g of sodium citrate, 0.45 to 4.0 g of dextrose, 0.01 to 0.3 g of penicillin, 50,000 to 500,000 IU of streptomycin, 0.25 to 0.45 g of catalase, 0.045 to 0.25 g of yolk, 1.5 to 15 ml of glycerol and 0.02 to 0.1 ⁇ g of dog serum.
- method for freezing dog sperm using the composition including the steps of (a) collecting male dog sperm by a massage method or electrical shock, (b) centrifuging the collected sperm to separate and remove the upper fluid leaving only the lower sperm, (c) mixing equal volumes of the lower sperm and a composition and primarily refrigerating the mixture, (d) further adding the composition to the mixture obtained in the step (c) to reduce the volumetric ratio of sperm to the composition to 1 :2, and secondarily refrigerating the resulting mixture, and (e) adding liquid nitrogen to the mixture obtained in the step (d) and freezing the mixture.
- a method for artificial insemination of dogs including the steps of (a) examining an appropriate ovulatory phase of a female dog for improvement of a fertility rate, (b) adding the frozen sperm prepared by the method of claim 2 to a mixture of a 0.3 to 1.5 wt% sodium chloride solution and dog serum, heated to a temperature of 30 to 40° C, and thawing the same, (c) testing the mobility of spermatozoa in the thawed sperm through a microscope, and (d) injecting the thawed sperm into the vagina of a female dog and massaging the clitoris of the female dog.
- the present inventor has extensively studied to develop a method for freezing dog sperm and compositions for use therein, and has found the fact that the dog sperm can be frozen for storage by combining a small amount of dog serum with a composition similar to a general diluent fluid used for cattle or pigs, to complete the invention.
- compositions for freezing dog sperm will first be described.
- compositions for freezing dog sperm according to the present invention are prepared by homogeneously mixing into 100 ml of distilled water, 0.25 to 4.5 g of sodium nitrate for regulating the buffering function, acidity (pH) and osmotic pressure of spermatozoa, 0.45 to 4.0 g of dextrose as a metabolism and energy source, 0.01 to 0.3 g of penicillin as an antibiotic for preventing infection, 50,000 to 500,000 IU of streptomycin, 0.25 to 0.45 g of catalase, 0.045 to 0.25 g of yolk,
- composition 1.5 to 15 ml of glycerol and 0.02 to 0.1 ⁇ g of dog serum.
- the thus prepared composition is frozen in units of about 2 ml to be thawed whenever it is to be used.
- composition according to the present invention stores sperm for a long time while maintaining the viability and motility of spermatozoa contained in the frozen sperm, and contains dog serum exhibiting normal fertility and delivery rates after thawing.
- spermatozoa die quickly when mixed with urine, it is preferred for a male dog to urinate before sperm is collected from the male dog. Then, the genital organ of the male dog is massaged using a test tube having an artificial vagina inserted therein to make the male dog ejaculate into the test tube. To catalyze the collection of sperm by this method, a female dog in heat is let to hover around the male dog. Otherwise, frozen cotton or a sponge containing a vaginal secretion of a female dog in heat is thawed and allowed to be smelled by the male dog when collecting sperm.
- the sperm is divided into three samples collected at different tubes.
- the sample of the sperm containing spermatozoa is the second one collected.
- Ea h sample is collected at a time interval of about 10 to 20 seconds.
- the interval between first and second samples is too short to be discernible.
- the second sample is preferably used.
- three samples are all collected in one test tube to be used.
- the thus collected sperm is separated by means of a centrifuge, and the upper fluid is discarded with only the lower sedimentary sperm left. Centrifuging is performed at about 500 to 1100 RPM/minute for 6 minutes.
- Equal volumes of the lower sedimentary sperm and the composition prepared as described above are mixed at room temperature and then the mixture is primarily refrigerated.
- the refrigerating time is about 10 to 20 minutes and the refrigerating temperature is about 5° C.
- a mixture of sperm and the composition in a volumetric ratio of 1 :2 is further mixed with the primarily refrigerated mixture at room temperature, and then secondarily refrigerated at the same temperature and for the same time.
- the sperm and the composition are homogeneously mixed.
- a predetermined amount of the secondarily refrigerated mixture is taken by means of a dropper and 2-3 drops are dropped to the surface of dry ice. Since the surface temperature of dry ice is about -79° C, the mixture is frozen to an extent. The frozen mixture is separated from dry ice and placed into a container having liquid nitrogen at about - 196° C to then be hermetically sealed, thereby completing the process of freezing dog sperm. This method is called a "pellet method" since the frozen mixture in the liquid nitrogen is pellet-shaped.
- the dog sperm can be frozen by a method using a straw, which will now be described briefly.
- the secondarily refrigerated mixture is subdivided into about 0.5 ml samples and put into straws. Every 20 to 30 straws are grouped and immersed in a container containing liquid nitrogen. When the straws are immersed into the container for freezing, they are gradually immersed, rather than immersed to the bottom of the container at once. First, they are immersed as deep as the upper portion of the container to then be left there for a predetermined time, then as deep as the middle of the container to then be left there for a predetermined time, and then as deep as the bottom of the container.
- the thus frozen sperm can be carried and stored for a long time, and can be used for artificial insemination after being thawed as needed.
- an artificial insemination method using the frozen sperm will be described.
- a test is performed to determine whether the female dog is in an ovulatory phase.
- the ovulatory phase test is performed by known methods in the art to which the present invention pertains, such as a vaginal cell test or a hormone test.
- the frozen sperm is thawed by adding the frozen sperm to a mixture of 0.3 to 1.5 wt% sodium chloride solution and a small amount of dog sperm heating, the mixture heated to a temperature of 30 to 40 °C, which is advantageous for maintaining the viability and shape of spermatozoa.
- the mixture may be directly heated, since it is quite difficult to appropriately control the temperature, it is preferred that an airtight container containing the mixture of sodium chloride solution and dog sperm is submerged in hot water at about 50° C and heated.
- the number, shape and mobility of living spermatozoa are examined through a microscope. Successful artificial insemination depends on the number of spermatozoa moving forward. Spermatozoa are classified into four types based on their mobility.
- Class 0 means the case where spermatozoa move their tales without forward movement
- class 1 means the case where less than 20% of spermatozoa move forward
- class 2 means the case where 20 to 50% of spermatozoa move forward
- class 3 means the case where 50 to 80% of spermatozoa move forward
- class 4 means the case where more than 80% of spermatozoa move forward.
- the thawed sperm is injected into the vagina of the female dog determined to be in the ovulatory phase and the clitoris of the female dog is massaged, to complete artificial insemination.
- the thawed sperm may be injected into the uterus of the female dog in a surgical manner.
- the lower part of the navel is cut about 2 cm to raise the uterus and the sperm of the male dog is directly injected into the uterus of the female dog by means of an injection syringe. Then, the peritoneum, abdominal muscle and skin of the female dog are sewed in order.
- a mixture having the following composition was homogeneously mixed using a mixer to prepare the composition for freezing dog sperm based on 100 ml of distilled water.
- Equal volumes of the thus separated lower sperm and the composition prepared in the above-described manner were mixed at room temperature and then primarily refrigerated.
- the refrigerating time was 10 to 20 minutes and the refrigerating temperature was about 5 ° C.
- the composition was further added to the primarily refrigerated mixture to then be mixed such that the mixture ratio of the sperm to the composition by volume became 1:2. This resulting mixture was then secondarily refrigerated at the same temperature and for the same time.
- a predetermined amount of the secondarily refrigerated mixture was taken by means of a dropper and 2-3 drops were dropped onto the surface of dry ice. Since the surface temperature of dry ice is about -79° C, the mixture was frozen to an extent. The frozen mixture was separated from the dry ice and placed into a container having liquid nitrogen of about - 196° C, to then be hermetically sealed, thereby completing the freezing process of dog sperm. Artificial insemination using frozen sperm
- Thawing was performed such that the frozen sperm was added to a mixture of 0.3 to 1.5 wt% sodium chloride solution and a small amount of dog sperm, the mixture was heated to a temperature of 38 °C and was then let to sit for 5 hours.
- the mobility of living spermatozoa was examined by placing the thawed sperm on a slide and observing the same through a microscope.
- Successful artificial insemination depends on the number of spermatozoa moving forward. The percentage of spermatozoa moving forward was about 85 % , which is suitable for artificial insemination.
- the thawed sperm was divided into three portions and then injected into the vaginas of female dogs determined to be in an ovulatory phase. Then, the clitoris of each of the female dogs was massaged for 5 minutes, to complete artificial insemination.
- a pregnancy test was done 1 month after completion of artificial insemination, and all three female dogs turned out to be pregnant. Later, the three female dogs delivered 4, 6 and 9 healthy puppies, respectively .
- EXAMPLE 2 the composition for freezing dog sperm was prepared in the same manner as in Example 1, with the exception of 0.02 ⁇ g of dog serum being used.
- Example 2 the freezing of dog sperm and artificial insemination were performed using the composition, by the same process as described in Example 1.
- Example 2 the same composition for freezing dog sperm as in Example 1 was prepared and the dog sperm was frozen in the same manner as in Example, with the exception of the volumetric ratio of the composition to the dog sperm being 1: 1. The dog sperm was collected in the same manner as in Example
- the frozen sperm was thawed in the same manner as in Example 1 and the mobility of spermatozoa in the thawed sperm was tested.
- the test result showed that the percentage of spermatozoa moving forward was less than 20% , which is not suitable for artificial insemination.
- Example 2 the same composition for freezing dog sperm was prepared in the same manner as in Example 1 , with the exception of dog serum not being contained in the composition.
- the freezing and thawing methods were the same as those in Example 1 , and the dog sperm was collected in the same manner as in Example 1 using the same male dog.
- the mobility test of spermatozoa frozen and thawed according to this comparative example showed that the spermatozoa move with their tales only without forward movement, which is not suitable for artificial insemination.
- the present invention provides a method for freezing dog sperm which is difficult to be frozen for storage in view of peculiarity of spermatozoa of the dog sperm, and compositions for use therein. Therefore, the dog sperm can be stored in a frozen state, thereby preserving the dog of good strain. Also, the frozen male dog sperm can be used for artificial insemination at an appropriate time and place, thereby enabling an increase in the birth rate.
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU44351/00A AU773578B2 (en) | 1999-04-28 | 2000-04-26 | Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm |
JP2000614915A JP2002543101A (en) | 1999-04-28 | 2000-04-26 | Composition for freezing dog semen, method for freezing dog semen using the same, and method for artificial insemination using the frozen dog semen |
GB0126189A GB2364233B (en) | 1999-04-28 | 2000-04-26 | Composition for freezing dog sperm method of freezing the dog sperm utilizing the compositions and artificial insemination method employing e frozen dog spe |
DE10084534T DE10084534B4 (en) | 1999-04-28 | 2000-04-26 | Method for freezing dog-seed liquid and use of frozen dog-seed liquid |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019990015147A KR19990078560A (en) | 1999-04-28 | 1999-04-28 | freezing method of dog sperm and artificial insemination method utilizing the sperm and compound for freezing the sperm thereof |
KR1999/15147 | 1999-04-28 | ||
KR2000/21012 | 2000-04-20 | ||
KR1020000021012A KR100320588B1 (en) | 1999-04-28 | 2000-04-20 | Compositions for freezing dog sperm, freezing methods of the dog sperm utilizing the compositions and artificial insemination method empolying the freezed dog sperm |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000066028A1 true WO2000066028A1 (en) | 2000-11-09 |
Family
ID=26635013
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2000/000396 WO2000066028A1 (en) | 1999-04-28 | 2000-04-26 | Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm |
Country Status (7)
Country | Link |
---|---|
JP (1) | JP2002543101A (en) |
KR (1) | KR100320588B1 (en) |
CN (1) | CN1170511C (en) |
AU (1) | AU773578B2 (en) |
DE (1) | DE10084534B4 (en) |
GB (1) | GB2364233B (en) |
WO (1) | WO2000066028A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101064415B1 (en) * | 2010-04-30 | 2011-09-14 | (주)미래생명공학연구소 | Method for treatment of spermatozoa in external fertilization of domestic animal |
CN104758084A (en) * | 2015-04-20 | 2015-07-08 | 陈绪凯 | Method for artificial insemination for cow through sex control and semen freezing |
ES2695248A1 (en) * | 2017-06-26 | 2019-01-02 | Spermbiobank Slu | METHOD AND KIT FOR FREEZING AND TRANSPORTATION OF SEMEN (Machine-translation by Google Translate, not legally binding) |
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KR100433280B1 (en) * | 2000-09-22 | 2004-05-31 | 대한민국 | Diluent for Boar Sperm Freezing |
KR20030014891A (en) * | 2001-08-13 | 2003-02-20 | (주)베티즌 | Compositions for cooling and cryopreservation of canine sperm, Methods for cooling and cryopreservation using the compositions, and Canine Articificial Insemination |
CN1331390C (en) * | 2003-12-24 | 2007-08-15 | 甘孜州家畜改良站 | Method for making frozen spermatozoon of yak |
CN100405902C (en) * | 2004-02-20 | 2008-07-30 | 辽宁省辽宁绒山羊原种场 | Frozen semen of animal and its making method |
CN100563665C (en) * | 2006-09-11 | 2009-12-02 | 华中农业大学 | A kind of cow sex-control sperm freezing preservation seminal fluid dilution |
CN102379279A (en) * | 2011-11-17 | 2012-03-21 | 张大宇 | Refrigerating fluid for storing Tibetan spaniel sperm |
CN103141471B (en) * | 2013-02-01 | 2015-09-30 | 四川农业大学 | A kind of Kunming hangquan semen freezing formula of liquid and compound method |
CN103900884B (en) * | 2014-04-11 | 2016-09-14 | 深圳市博锐德生物科技有限公司 | Refining is combined quality-control product and preparation method thereof, test kit |
PL3528745T3 (en) | 2016-10-18 | 2020-11-30 | Smartbow Gmbh | Device for artificially inseminating a mammal |
CN107251892A (en) * | 2017-05-25 | 2017-10-17 | 中国热带农业科学院热带作物品种资源研究所 | It is a kind of suitable for the solution for dilution of cock semen of torrid areas, preparation and its application process |
CN107494520B (en) * | 2017-09-25 | 2020-07-28 | 江苏农牧科技职业学院 | Dog semen freezing diluent and freezing method |
KR102536863B1 (en) | 2019-11-25 | 2023-05-30 | 서울대학교산학협력단 | A Composition for semen cryoprotectant of animal including amniotic membrane derived stem cell-conditioned media and method of making thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4007087A (en) * | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
SU587934A1 (en) * | 1973-07-19 | 1978-01-15 | Украинский Научно-Исследовательский Институт Разведения И Искусственного Осеменения Крупного Рогатого Скота | Animal sperm dilutant |
SE7800809L (en) * | 1977-01-26 | 1978-07-27 | Fisons Ltd | PROCEDURE FOR THE PREPARATION OF NEW SALTS OF BENZOPYRANE DERIVATIVES |
JPH0826901A (en) * | 1994-07-11 | 1996-01-30 | Rikagaku Kenkyusho | Agent for protecting sperm at low temperature |
KR0160090B1 (en) * | 1995-05-01 | 1999-02-18 | 김광희 | Artificial insemination diluent for liquid sperm of pig |
-
2000
- 2000-04-20 KR KR1020000021012A patent/KR100320588B1/en not_active IP Right Cessation
- 2000-04-26 GB GB0126189A patent/GB2364233B/en not_active Expired - Fee Related
- 2000-04-26 CN CNB008080003A patent/CN1170511C/en not_active Expired - Fee Related
- 2000-04-26 JP JP2000614915A patent/JP2002543101A/en active Pending
- 2000-04-26 DE DE10084534T patent/DE10084534B4/en not_active Expired - Fee Related
- 2000-04-26 WO PCT/KR2000/000396 patent/WO2000066028A1/en active Application Filing
- 2000-04-26 AU AU44351/00A patent/AU773578B2/en not_active Ceased
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU587934A1 (en) * | 1973-07-19 | 1978-01-15 | Украинский Научно-Исследовательский Институт Разведения И Искусственного Осеменения Крупного Рогатого Скота | Animal sperm dilutant |
US4007087A (en) * | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
SE7800809L (en) * | 1977-01-26 | 1978-07-27 | Fisons Ltd | PROCEDURE FOR THE PREPARATION OF NEW SALTS OF BENZOPYRANE DERIVATIVES |
JPH0826901A (en) * | 1994-07-11 | 1996-01-30 | Rikagaku Kenkyusho | Agent for protecting sperm at low temperature |
KR0160090B1 (en) * | 1995-05-01 | 1999-02-18 | 김광희 | Artificial insemination diluent for liquid sperm of pig |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101064415B1 (en) * | 2010-04-30 | 2011-09-14 | (주)미래생명공학연구소 | Method for treatment of spermatozoa in external fertilization of domestic animal |
CN104758084A (en) * | 2015-04-20 | 2015-07-08 | 陈绪凯 | Method for artificial insemination for cow through sex control and semen freezing |
ES2695248A1 (en) * | 2017-06-26 | 2019-01-02 | Spermbiobank Slu | METHOD AND KIT FOR FREEZING AND TRANSPORTATION OF SEMEN (Machine-translation by Google Translate, not legally binding) |
WO2019002640A1 (en) * | 2017-06-26 | 2019-01-03 | Spermbiobank, Slu | Method and kit for freezing and transporting semen |
Also Published As
Publication number | Publication date |
---|---|
GB0126189D0 (en) | 2002-01-02 |
KR100320588B1 (en) | 2002-01-12 |
DE10084534B4 (en) | 2007-09-13 |
CN1170511C (en) | 2004-10-13 |
GB2364233A (en) | 2002-01-23 |
JP2002543101A (en) | 2002-12-17 |
GB2364233B (en) | 2003-06-25 |
AU773578B2 (en) | 2004-05-27 |
KR20000049647A (en) | 2000-08-05 |
CN1351484A (en) | 2002-05-29 |
DE10084534T1 (en) | 2002-05-02 |
AU4435100A (en) | 2000-11-17 |
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