WO2000066092A3 - Method for producing a polyurethane matrix with covalently immobilised biomolecules - Google Patents

Method for producing a polyurethane matrix with covalently immobilised biomolecules Download PDF

Info

Publication number
WO2000066092A3
WO2000066092A3 PCT/EP2000/003830 EP0003830W WO0066092A3 WO 2000066092 A3 WO2000066092 A3 WO 2000066092A3 EP 0003830 W EP0003830 W EP 0003830W WO 0066092 A3 WO0066092 A3 WO 0066092A3
Authority
WO
WIPO (PCT)
Prior art keywords
polyurethane matrix
producing
covalently immobilised
biomolecules
immobilised biomolecules
Prior art date
Application number
PCT/EP2000/003830
Other languages
German (de)
French (fr)
Other versions
WO2000066092A2 (en
Inventor
Norbert Ettner
Thomas Mahler
Ernst Schaumann
Michael Schink
Original Assignee
Beiersdorf Ag
Norbert Ettner
Thomas Mahler
Ernst Schaumann
Michael Schink
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beiersdorf Ag, Norbert Ettner, Thomas Mahler, Ernst Schaumann, Michael Schink filed Critical Beiersdorf Ag
Priority to AU45587/00A priority Critical patent/AU4558700A/en
Priority to EP00927092A priority patent/EP1173156A2/en
Publication of WO2000066092A2 publication Critical patent/WO2000066092A2/en
Publication of WO2000066092A3 publication Critical patent/WO2000066092A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G18/00—Polymeric products of isocyanates or isothiocyanates
    • C08G18/06—Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen
    • C08G18/83—Chemically modified polymers
    • C08G18/85—Chemically modified polymers by azo compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/093—Polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G18/00—Polymeric products of isocyanates or isothiocyanates
    • C08G18/06—Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen
    • C08G18/08—Processes
    • C08G18/0838—Manufacture of polymers in the presence of non-reactive compounds
    • C08G18/0842—Manufacture of polymers in the presence of non-reactive compounds in the presence of liquid diluents
    • C08G18/0847—Manufacture of polymers in the presence of non-reactive compounds in the presence of liquid diluents in the presence of solvents for the polymers
    • C08G18/0852—Manufacture of polymers in the presence of non-reactive compounds in the presence of liquid diluents in the presence of solvents for the polymers the solvents being organic
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2220/00—Compositions for preparing gels other than hydrogels, aerogels and xerogels

Abstract

According to the inventive method for producing a polyurethane matrix with covalently immobilised biomolecules, the polyurethane matrix is presented in a solvent mixture containing an at least 80 % non polar solvent in which the polyurethane matrix does not swell or swells only slightly and in which the activating reagent is contained, so that the subsequent immobilisation of the biomolecule preferably takes place on the surface of the polyurethane matrix or on layers near to the surface.
PCT/EP2000/003830 1999-05-04 2000-04-27 Method for producing a polyurethane matrix with covalently immobilised biomolecules WO2000066092A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU45587/00A AU4558700A (en) 1999-05-04 2000-04-27 Method for producing a polyurethane matrix with covalently immobilised biomolecules
EP00927092A EP1173156A2 (en) 1999-05-04 2000-04-27 Method for producing a polyurethane matrix with covalently immobilised biomolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19920262A DE19920262A1 (en) 1999-05-04 1999-05-04 Process for the production of a polyurethane matrix with covalently immobilized biomolecules
DE19920262.1 1999-05-04

Publications (2)

Publication Number Publication Date
WO2000066092A2 WO2000066092A2 (en) 2000-11-09
WO2000066092A3 true WO2000066092A3 (en) 2001-05-31

Family

ID=7906789

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/003830 WO2000066092A2 (en) 1999-05-04 2000-04-27 Method for producing a polyurethane matrix with covalently immobilised biomolecules

Country Status (4)

Country Link
EP (1) EP1173156A2 (en)
AU (1) AU4558700A (en)
DE (1) DE19920262A1 (en)
WO (1) WO2000066092A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10019380A1 (en) * 2000-04-19 2001-10-25 Basf Ag Process for the production of covalently bound biologically active substances on polyurethane foams and use of the supported polyurethane foams for chiral syntheses

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098645A (en) * 1976-02-24 1978-07-04 W. R. Grace & Co. Immobilization of proteins with polyurethane polymers
US4250267A (en) * 1977-11-10 1981-02-10 W. R. Grace & Co. Immobilized biological material
US5134072A (en) * 1987-02-23 1992-07-28 Boehringer Mannheim Gmbh Preparation of a water-soluble modified enzyme by covalently bonding an enzyme to a polyurethane pre-polymer/bisulfite adduct
DE4308445A1 (en) * 1993-03-17 1994-09-22 Beiersdorf Ag Wound dressing based on hydrophilic polyurethane gel foams and process for their production

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1173387A (en) * 1980-09-17 1984-08-28 Wayne E. Swann Process for production of l-aspartic acids using immobilized microorganisms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098645A (en) * 1976-02-24 1978-07-04 W. R. Grace & Co. Immobilization of proteins with polyurethane polymers
US4250267A (en) * 1977-11-10 1981-02-10 W. R. Grace & Co. Immobilized biological material
US5134072A (en) * 1987-02-23 1992-07-28 Boehringer Mannheim Gmbh Preparation of a water-soluble modified enzyme by covalently bonding an enzyme to a polyurethane pre-polymer/bisulfite adduct
DE4308445A1 (en) * 1993-03-17 1994-09-22 Beiersdorf Ag Wound dressing based on hydrophilic polyurethane gel foams and process for their production

Also Published As

Publication number Publication date
AU4558700A (en) 2000-11-17
EP1173156A2 (en) 2002-01-23
WO2000066092A2 (en) 2000-11-09
DE19920262A1 (en) 2000-11-09

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