WO2001010886A3 - Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration - Google Patents

Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration Download PDF

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Publication number
WO2001010886A3
WO2001010886A3 PCT/EP2000/007478 EP0007478W WO0110886A3 WO 2001010886 A3 WO2001010886 A3 WO 2001010886A3 EP 0007478 W EP0007478 W EP 0007478W WO 0110886 A3 WO0110886 A3 WO 0110886A3
Authority
WO
WIPO (PCT)
Prior art keywords
solutions
obtaining
relates
vacuum filtration
protein purification
Prior art date
Application number
PCT/EP2000/007478
Other languages
English (en)
French (fr)
Other versions
WO2001010886A2 (de
Inventor
Joachim Ribbe
Frank Schaefer
Kerstin Steinert
Helge Lubenow
Original Assignee
Qiagen Gmbh
Joachim Ribbe
Frank Schaefer
Kerstin Steinert
Helge Lubenow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=7917472&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2001010886(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Qiagen Gmbh, Joachim Ribbe, Frank Schaefer, Kerstin Steinert, Helge Lubenow filed Critical Qiagen Gmbh
Priority to EP00949438A priority Critical patent/EP1206483B1/de
Priority to JP2001515694A priority patent/JP4786094B2/ja
Priority to AT00949438T priority patent/ATE244724T1/de
Priority to DE50002853T priority patent/DE50002853D1/de
Priority to DK00949438T priority patent/DK1206483T3/da
Priority to US10/049,003 priority patent/US6864100B1/en
Publication of WO2001010886A2 publication Critical patent/WO2001010886A2/de
Publication of WO2001010886A3 publication Critical patent/WO2001010886A3/de

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D19/00—Degasification of liquids
    • B01D19/02—Foam dispersion or prevention
    • B01D19/04—Foam dispersion or prevention by addition of chemical substances
    • B01D19/0404—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance
    • B01D19/0409—Foam dispersion or prevention by addition of chemical substances characterised by the nature of the chemical substance compounds containing Si-atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Die Erfindung betrifft ein Verfahren sowie ein Reagenzienkit zur Gewinnung von Zellinhaltsstoffe enthaltenden klaren Lösungen aus biologischen Proben im Hochdurchsatzverfahren, umfassend die Schritte: Bereitstellen von mehreren proteinhaltigen Lösungen, die unlösliche Bestandteile enthalten, in separaten Kammern einer Multikammer-Filtrationseinheit, Entfernen von unlöslichen Bestandteilen durch Filtrieren der Lösungen durch die Multikammer-Filtrationseinheit unter Anlegen einer Druckdifferenz, wobei durch chemische oder/und mechanische Mittel eine Kreuzkontamination benachbarter Kammern verhindert wird, und Auffangen der einzelnen Filtrate jeweils separat in Auffangbehältern, sowie ein Verfahren zur Gewinnung von Zellinhaltsstoffen aus den erhaltenen klaren Lösungen. Weiterhin betrifft die Erfindung die Verwendung einer Vorrichtung zur Gewinnung von klaren proteinhaltigen Lösungen aus biologischen Proben.
PCT/EP2000/007478 1999-08-06 2000-08-02 Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration WO2001010886A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00949438A EP1206483B1 (de) 1999-08-06 2000-08-02 Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration
JP2001515694A JP4786094B2 (ja) 1999-08-06 2000-08-02 マルチウェルにおける減圧濾過による自動タンパク質精製
AT00949438T ATE244724T1 (de) 1999-08-06 2000-08-02 Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration
DE50002853T DE50002853D1 (de) 1999-08-06 2000-08-02 Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration
DK00949438T DK1206483T3 (da) 1999-08-06 2000-08-02 Automatiseret proteinrensning i mangebrøndsformat ved vakuumfiltrering
US10/049,003 US6864100B1 (en) 1999-08-06 2000-08-02 Automated protein purification the multiwell format by vacuum filtration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19937187.3 1999-08-06
DE19937187A DE19937187A1 (de) 1999-08-06 1999-08-06 Automatisierte Proteinreinigung im Multiwellformat durch Vakuumfiltration

Publications (2)

Publication Number Publication Date
WO2001010886A2 WO2001010886A2 (de) 2001-02-15
WO2001010886A3 true WO2001010886A3 (de) 2001-09-07

Family

ID=7917472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/007478 WO2001010886A2 (de) 1999-08-06 2000-08-02 Automatisierte proteinreinigung im multiwellformat durch vakuumfiltration

Country Status (8)

Country Link
US (1) US6864100B1 (de)
EP (1) EP1206483B1 (de)
JP (1) JP4786094B2 (de)
AT (1) ATE244724T1 (de)
DE (2) DE19937187A1 (de)
DK (1) DK1206483T3 (de)
ES (1) ES2202153T3 (de)
WO (1) WO2001010886A2 (de)

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US20030087293A1 (en) * 2001-10-23 2003-05-08 Decode Genetics Ehf. Nucleic acid isolation method and apparatus for performing same
DE10241938A1 (de) * 2002-09-10 2004-03-25 Noxxon Pharma Ag Verfahren zur Selektion von Nukleinsäureliganden
WO2005026347A1 (en) * 2003-09-09 2005-03-24 Fuji Photo Film Co., Ltd. Method for isolating and purifying a nucleic acid
US7240572B2 (en) 2004-02-18 2007-07-10 Millipore Corporation Vacuum assisted affinity chromatography device and method
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US20060105349A1 (en) * 2004-11-12 2006-05-18 Promega Corporation Device and method for purification of biological materials
US20060105391A1 (en) * 2004-11-12 2006-05-18 Promega Corporation Device and method for separating molecules
DE102005001362A1 (de) * 2005-01-11 2006-07-20 Protagen Ag Verfahren und Vorrichtung zur Quantifizierung von Proteinen
JP5156738B2 (ja) * 2006-06-06 2013-03-06 エフ．ホフマン−ラ ロシュ アーゲー 全血試料の特異的溶血
US8357538B2 (en) * 2007-04-06 2013-01-22 Qiagen Gaithersburg, Inc. Automated assay and system
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DE102008030142A1 (de) * 2008-06-27 2009-12-31 Qiagen Gmbh Verfahren zur Proteinaufreinigung unter denaturierenden Bedingungen
WO2011063139A1 (en) 2009-11-18 2011-05-26 Qiagen Laboratory central control unit method and system
US8307490B2 (en) * 2010-02-24 2012-11-13 Allway Tools, Inc. Multifunction caulk tool
EP2371731A1 (de) * 2010-03-31 2011-10-05 Roche Diagnostics GmbH Reagenz-Kit mit Transportsicherung
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WO2013056253A1 (en) * 2011-10-15 2013-04-18 The Regents Of The University Of California High throughput instrumentation to screen cells and particles based on their mechanical properties
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EP2687592A1 (de) * 2012-07-20 2014-01-22 greenovation Biotech GmbH Filtration von Zellkulturüberständen
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US9758755B2 (en) 2015-10-23 2017-09-12 Life Technologies Corporation Filter-based method for efficient capture of lysis of suspended cells
WO2017069781A1 (en) * 2015-10-23 2017-04-27 Life Technologies Corporation Filter-based system and method for efficient capture and lysis of suspended cells
KR102599895B1 (ko) 2017-03-22 2023-11-07 언체인드 랩스 버퍼 교환을 위한 샘플 플레이트들 및 제조 방법
US11300573B2 (en) * 2017-08-03 2022-04-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Means and methods for protein quantification

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Also Published As

Publication number Publication date
DK1206483T3 (da) 2003-10-27
DE50002853D1 (de) 2003-08-14
ES2202153T3 (es) 2004-04-01
ATE244724T1 (de) 2003-07-15
EP1206483A2 (de) 2002-05-22
JP2003506456A (ja) 2003-02-18
US6864100B1 (en) 2005-03-08
EP1206483B1 (de) 2003-07-09
DE19937187A1 (de) 2001-03-01
JP4786094B2 (ja) 2011-10-05
WO2001010886A2 (de) 2001-02-15

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