WO2001021310A3 - Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification - Google Patents

Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification Download PDF

Info

Publication number
WO2001021310A3
WO2001021310A3 PCT/US2000/025851 US0025851W WO0121310A3 WO 2001021310 A3 WO2001021310 A3 WO 2001021310A3 US 0025851 W US0025851 W US 0025851W WO 0121310 A3 WO0121310 A3 WO 0121310A3
Authority
WO
WIPO (PCT)
Prior art keywords
thermocycling
dialysis
purification
sample processing
dna sample
Prior art date
Application number
PCT/US2000/025851
Other languages
French (fr)
Other versions
WO2001021310A9 (en
WO2001021310A2 (en
Inventor
Patrick Cahill
Douglas Smith
Ulrich Thomann
Marcy Engelstein
Original Assignee
Genome Therapeutics Corp
Patrick Cahill
Douglas Smith
Ulrich Thomann
Marcy Engelstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genome Therapeutics Corp, Patrick Cahill, Douglas Smith, Ulrich Thomann, Marcy Engelstein filed Critical Genome Therapeutics Corp
Priority to AU75979/00A priority Critical patent/AU7597900A/en
Priority to CA002385029A priority patent/CA2385029A1/en
Priority to EP00965231A priority patent/EP1214149A2/en
Priority to JP2001524729A priority patent/JP2004500552A/en
Publication of WO2001021310A2 publication Critical patent/WO2001021310A2/en
Publication of WO2001021310A3 publication Critical patent/WO2001021310A3/en
Publication of WO2001021310A9 publication Critical patent/WO2001021310A9/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B01L9/065—Test-tube stands; Test-tube holders specially adapted for capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/24—Dialysis ; Membrane extraction
    • B01D61/28—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/02—Hollow fibre modules
    • B01D63/024—Hollow fibre modules with a single potted end
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/02—Hollow fibre modules
    • B01D63/026—Wafer type modules or flat-surface type modules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50857—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/04—Specific sealing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/04—Specific sealing means
    • B01D2313/041—Gaskets or O-rings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/06—External membrane module supporting or fixing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/12—Specific discharge elements
    • B01D2313/125—Discharge manifolds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/54—Modularity of membrane module elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2319/00—Membrane assemblies within one housing
    • B01D2319/06—Use of membranes of different materials or properties within one module
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B01J2219/00369—Pipettes capillary in multiple or parallel arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/004—Pinch valves
    • B01J2219/00403—Pinch valves in multiple arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks

Abstract

The present invention provides, in one embodiment, a capillary cassette to facilitate rapid dialysis or thermocycling, via liquid or gas, of sub-microliter samples. In one embodiment, the invention is configured for use with a fluid handling machine for automated, high-throughput processing. Dialysis or thermocycling can be performed while the capillary cassette is mounted on the fluid handling machine. However, another embodiment of the invention provides higher throughput by the use of a hotel to simultaneously process a plurality of capillary cassettes. Hotel capabilities include dialysis, thermocycling, cleaning and chemical regeneration. Exemplary applications include but are not limited to polymerase chain reaction (PCR), DNA sequencing applications, oligonucleotide ligation, ligase chain reaction (LCR), single nucleotide extension, exonuclease treatment, and oligonucleotide hybridization assays.
PCT/US2000/025851 1999-09-21 2000-09-21 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification WO2001021310A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU75979/00A AU7597900A (en) 1999-09-21 2000-09-21 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
CA002385029A CA2385029A1 (en) 1999-09-21 2000-09-21 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
EP00965231A EP1214149A2 (en) 1999-09-21 2000-09-21 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
JP2001524729A JP2004500552A (en) 1999-09-21 2000-09-21 An instrument for rapid DNA sample processing that integrates liquid processing, thermal cycling and purification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15529999P 1999-09-21 1999-09-21
US60/155,299 1999-09-21

Publications (3)

Publication Number Publication Date
WO2001021310A2 WO2001021310A2 (en) 2001-03-29
WO2001021310A3 true WO2001021310A3 (en) 2002-01-17
WO2001021310A9 WO2001021310A9 (en) 2002-10-03

Family

ID=22554862

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/025851 WO2001021310A2 (en) 1999-09-21 2000-09-21 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification

Country Status (5)

Country Link
EP (1) EP1214149A2 (en)
JP (1) JP2004500552A (en)
AU (1) AU7597900A (en)
CA (1) CA2385029A1 (en)
WO (1) WO2001021310A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0202415D0 (en) * 2001-12-11 2002-08-13 Thomas Laurell Dockable processing module
US6833238B2 (en) 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
EP1525053A1 (en) * 2002-07-26 2005-04-27 Applera Corporation Device and method for purification of nucleic acids
DE10307227A1 (en) * 2003-02-14 2004-08-26 Cytocentrics Ccs Gmbh Introducing fluid into microfluidic structures for e.g. analysis or synthesis, locates elastic material above access opening and penetrates it using hollow needle
DE10349513B4 (en) * 2003-10-23 2006-08-31 Eads Space Transportation Gmbh experimental apparatus
US7250260B2 (en) 2004-02-18 2007-07-31 Applera Corporation Multi-step bioassays on modular microfluidic application platforms
WO2006043181A2 (en) * 2004-08-04 2006-04-27 Spinx, Inc. Devices and methods for interfacing microfluidic devices with fluid handling devices
DE102005026585A1 (en) * 2005-05-03 2006-11-09 Forschungszentrum Jülich GmbH Method for sample introduction into modules as well as adapters
EP1925364A1 (en) * 2006-11-23 2008-05-28 Nederlandse Organisatie voor Toegepast-Natuuurwetenschappelijk Onderzoek TNO Multiple microfluidic connector
ES2538691T3 (en) * 2012-02-24 2015-06-23 F. Hoffmann-La Roche Ag Septum closure
US20150137015A1 (en) * 2012-07-12 2015-05-21 Agency For Science, Technology And Research Connector for microfluidic device, a method for injecting fluid into microfluidic device using the connector and a method of providing and operating a valve
DK2848309T3 (en) * 2013-09-13 2020-09-07 Nanotemper Tech Gmbh CARRIER FOR CAPITAL PIPES
KR20160114494A (en) * 2015-03-24 2016-10-05 삼성전자주식회사 Microfluidic device and apparatus for analyzing sample
ES2843532T3 (en) * 2015-07-23 2021-07-19 Cepheid Thermal control device and methods of use
CA3107571A1 (en) * 2018-06-27 2020-01-02 Qcdx Llc Biological sample holder and handler
GB201818936D0 (en) * 2018-11-21 2019-01-02 Univ Loughborough Microfluid device
CN112368077B (en) * 2018-12-07 2023-11-24 元素生物科学公司 Flow cell device and use thereof
US20230098565A1 (en) * 2020-01-31 2023-03-30 Western Connecticut Health Network, Inc. Apparatus and method for collecting liquid samples
CN114854570B (en) * 2022-07-05 2022-09-02 鲲鹏基因（北京）科技有限责任公司 Temperature control device, liquid cooling temperature control system and PCR instrument
GB202212732D0 (en) * 2022-09-01 2022-10-19 Remedy Biologics Ltd Methods and systems of material recovery

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3915652A (en) * 1973-08-16 1975-10-28 Samuel Natelson Means for transferring a liquid in a capillary open at both ends to an analyzing system
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
WO1998004920A2 (en) * 1996-07-29 1998-02-05 Idexx Laboratories, Inc. Immunoassays in capillary tubes
US5785926A (en) * 1995-09-19 1998-07-28 University Of Washington Precision small volume fluid processing apparatus
US5840573A (en) * 1994-02-01 1998-11-24 Fields; Robert E. Molecular analyzer and method of use

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3915652A (en) * 1973-08-16 1975-10-28 Samuel Natelson Means for transferring a liquid in a capillary open at both ends to an analyzing system
US5840573A (en) * 1994-02-01 1998-11-24 Fields; Robert E. Molecular analyzer and method of use
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
US5785926A (en) * 1995-09-19 1998-07-28 University Of Washington Precision small volume fluid processing apparatus
WO1998004920A2 (en) * 1996-07-29 1998-02-05 Idexx Laboratories, Inc. Immunoassays in capillary tubes

Also Published As

Publication number Publication date
WO2001021310A9 (en) 2002-10-03
AU7597900A (en) 2001-04-24
EP1214149A2 (en) 2002-06-19
CA2385029A1 (en) 2001-03-29
WO2001021310A2 (en) 2001-03-29
JP2004500552A (en) 2004-01-08

Similar Documents

Publication Publication Date Title
WO2001021310A3 (en) Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
US10378049B2 (en) Apparatus and methods for parallel processing of microvolume liquid reactions
US9170060B2 (en) Rapid microfluidic thermal cycler for nucleic acid amplification
US7332271B2 (en) Apparatus and methods for parallel processing of micro-volume liquid reactions
US5587128A (en) Mesoscale polynucleotide amplification devices
KR100483684B1 (en) Kit for separating and purifying nucleic acids or various biological materials, and system for automatically performing separation or purification of biological materials using the same
EP0739423B1 (en) Mesoscale polynucleotide amplification devices
US20170100719A1 (en) Cartridge, system and method for automated medical diagnostics
US9464319B2 (en) Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US6875619B2 (en) Microfluidic devices comprising biochannels
JP2001521622A (en) Closed-loop biochemical analyzer
US20020106787A1 (en) Device for repid DNA sample processing with integrated liquid handling, thermocycling, and purification
WO2004001376A2 (en) Apparatus for polynucleotide detection and quantitation
WO2010118430A1 (en) A method of delivering pcr solution to microfluidic pcr chamber
WO2000066995A3 (en) Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
WO2002016652A2 (en) Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
US20170051365A9 (en) Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
Dunker et al. Parallel DNA template preparation using a vacuum filtration sample transfer device
Eckersten et al. High-throughput SNP scoring in a disposable microfabricated CD device
JP4666133B2 (en) Nucleic acid detector
AU698213C (en) Mesoscale polynucleotide amplification devices
Barlocchi et al. Silicon micromachining for lab on chip
WO2005060345A2 (en) New method for sequencing-by-synthesis

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 75979/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2385029

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 517820

Country of ref document: NZ

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 524729

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000965231

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000965231

Country of ref document: EP

AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/14-14/14, DRAWINGS, REPLACED BY NEW PAGES 1/15-15/15; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWW Wipo information: withdrawn in national office

Ref document number: 2000965231

Country of ref document: EP