WO2001035071A3 - Cell analysis and sorting apparatus for manipulation of cells - Google Patents

Cell analysis and sorting apparatus for manipulation of cells Download PDF

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Publication number
WO2001035071A3
WO2001035071A3 PCT/US2000/030815 US0030815W WO0135071A3 WO 2001035071 A3 WO2001035071 A3 WO 2001035071A3 US 0030815 W US0030815 W US 0030815W WO 0135071 A3 WO0135071 A3 WO 0135071A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
cells
sorting apparatus
cell analysis
manipulation
Prior art date
Application number
PCT/US2000/030815
Other languages
French (fr)
Other versions
WO2001035071A2 (en
Inventor
Rebecca Braff
Joel Voldman
Martha L Gray
Martin A Schmidt
Mehmet Toner
Original Assignee
Massachusetts Inst Technology
Rebecca Braff
Joel Voldman
Martha L Gray
Martin A Schmidt
Mehmet Toner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology, Rebecca Braff, Joel Voldman, Martha L Gray, Martin A Schmidt, Mehmet Toner filed Critical Massachusetts Inst Technology
Priority to CA002390375A priority Critical patent/CA2390375A1/en
Priority to AU15925/01A priority patent/AU1592501A/en
Priority to EP00978464A priority patent/EP1236032A2/en
Priority to JP2001536950A priority patent/JP2003514236A/en
Publication of WO2001035071A2 publication Critical patent/WO2001035071A2/en
Publication of WO2001035071A3 publication Critical patent/WO2001035071A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • G01N15/149—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
    • G01N2015/1415—Control of particle position

Abstract

A cell analysis and sorting apparatus is capable of monitoring over time the behaviour of each cell in a large population of cells. The cell analysis and sorting apparatus contains individually addressable cell locations. Each location is capable of capturing and holding a single cell, and selectively releasing that cell from that particular location. In one aspect of the invention, the cells are captured and held in wells, and released using vapor bubbles as a means of cell actuation. In another aspect of the invention, the cells are captured, held and released using electric fields traps.
PCT/US2000/030815 1999-11-10 2000-11-10 Cell analysis and sorting apparatus for manipulation of cells WO2001035071A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002390375A CA2390375A1 (en) 1999-11-10 2000-11-10 Cell analysis and sorting apparatus for manipulation of cells
AU15925/01A AU1592501A (en) 1999-11-10 2000-11-10 Cell analysis and sorting apparatus for manipulation of cells
EP00978464A EP1236032A2 (en) 1999-11-10 2000-11-10 Cell analysis and sorting apparatus for manipulation of cells
JP2001536950A JP2003514236A (en) 1999-11-10 2000-11-10 Cell analysis and sorting equipment for manipulating cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16464399P 1999-11-10 1999-11-10
US60/164,643 1999-11-10

Publications (2)

Publication Number Publication Date
WO2001035071A2 WO2001035071A2 (en) 2001-05-17
WO2001035071A3 true WO2001035071A3 (en) 2002-02-21

Family

ID=22595424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/030815 WO2001035071A2 (en) 1999-11-10 2000-11-10 Cell analysis and sorting apparatus for manipulation of cells

Country Status (5)

Country Link
EP (1) EP1236032A2 (en)
JP (1) JP2003514236A (en)
AU (1) AU1592501A (en)
CA (1) CA2390375A1 (en)
WO (1) WO2001035071A2 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020064808A1 (en) * 2000-11-29 2002-05-30 Mutz Mitchell W. Focused acoustic energy for ejecting cells from a fluid
US6893836B2 (en) 2000-11-29 2005-05-17 Picoliter Inc. Spatially directed ejection of cells from a carrier fluid
IL154677A0 (en) 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
US7888110B2 (en) 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
JP2007520546A (en) 2004-02-03 2007-07-26 ケマジス リミティド A stable amorphous form of Montelukast sodium
BRPI0508527A (en) 2004-03-08 2007-08-14 Endoart Sa tubular system closure system
US7403647B2 (en) 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
WO2006080000A1 (en) * 2005-01-25 2006-08-03 Seng Enterprises Ltd. Device for the studying individual cells
GB0508983D0 (en) 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
EP4108780A1 (en) 2006-06-14 2022-12-28 Verinata Health, Inc. Rare cell analysis using sample splitting and dna tags
EP2029779A4 (en) 2006-06-14 2010-01-20 Living Microsystems Inc Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2237887A2 (en) 2007-12-26 2010-10-13 Seng Enterprises Ltd. Device for the study of living cells
JP2011000079A (en) * 2009-06-19 2011-01-06 Univ Of Tokyo Method for operating particle and micro fluid device
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
US9176504B2 (en) * 2011-02-11 2015-11-03 The Regents Of The University Of California High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
CN105264127B (en) 2013-03-15 2019-04-09 Gpb科学有限责任公司 The on piece microfluidic process of particle
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
CN113512522A (en) 2013-03-15 2021-10-19 普林斯顿大学理事会 Method and apparatus for high throughput purification
CN105452873B (en) 2013-03-15 2019-01-18 加利福尼亚大学董事会 Microfluid droplet is generated and is manipulated high speed on demand
JP6334274B2 (en) * 2014-06-03 2018-05-30 積水化学工業株式会社 Suspension transfer method
WO2016079269A1 (en) * 2014-11-19 2016-05-26 Imec Vzw Microbubble generator device, systems and method to fabricate
SG11201805473SA (en) * 2015-12-30 2018-07-30 Berkeley Lights Inc Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
US11229912B2 (en) 2018-03-27 2022-01-25 Hewlett-Packard Development Company, L.P. Particle separation
JP2020027068A (en) * 2018-08-15 2020-02-20 ソニー株式会社 Particle acquisition method, chamber for particle collection, and particle analysis system
EP3962652A4 (en) 2019-04-30 2023-01-18 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
US11492585B2 (en) 2019-05-31 2022-11-08 Canon Medical Systems Corporation Cell identification system and cell identification method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4894343A (en) * 1986-11-19 1990-01-16 Hitachi, Ltd. Chamber plate for use in cell fusion and a process for production thereof
US5506141A (en) * 1982-05-10 1996-04-09 Bar-Ilan University Apertured cell carrier
DE19712309A1 (en) * 1996-11-16 1998-05-20 Nmi Univ Tuebingen Microelement arrangement, method for contacting cells in a liquid environment and method for producing a microelement arrangement
WO1999031503A1 (en) * 1997-12-17 1999-06-24 Horst Vogel Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5506141A (en) * 1982-05-10 1996-04-09 Bar-Ilan University Apertured cell carrier
US4894343A (en) * 1986-11-19 1990-01-16 Hitachi, Ltd. Chamber plate for use in cell fusion and a process for production thereof
DE19712309A1 (en) * 1996-11-16 1998-05-20 Nmi Univ Tuebingen Microelement arrangement, method for contacting cells in a liquid environment and method for producing a microelement arrangement
WO1999031503A1 (en) * 1997-12-17 1999-06-24 Horst Vogel Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

Also Published As

Publication number Publication date
JP2003514236A (en) 2003-04-15
WO2001035071A2 (en) 2001-05-17
AU1592501A (en) 2001-06-06
EP1236032A2 (en) 2002-09-04
CA2390375A1 (en) 2001-05-17

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