WO2001036680A3 - METHODS OF PREPARING cRNA - Google Patents

METHODS OF PREPARING cRNA

Info

Publication number
WO2001036680A3
WO2001036680A3 PCT/US2000/031097 US0031097W WO0136680A3 WO 2001036680 A3 WO2001036680 A3 WO 2001036680A3 US 0031097 W US0031097 W US 0031097W WO 0136680 A3 WO0136680 A3 WO 0136680A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
preparing crna
crna
preparing
robust
Prior art date
Application number
PCT/US2000/031097
Other languages
French (fr)
Other versions
WO2001036680A2 (en
Inventor
Eric M Eastman
Larry S Millstein
Original Assignee
Eric M Eastman
Larry S Millstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eric M Eastman, Larry S Millstein filed Critical Eric M Eastman
Priority to AU16018/01A priority Critical patent/AU1601801A/en
Publication of WO2001036680A2 publication Critical patent/WO2001036680A2/en
Publication of WO2001036680A3 publication Critical patent/WO2001036680A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

New and improved methods are provided for generating cRNA molecules form cellular mRNA. The methods are robust and reliable, and can be used to provide gene fragments for use in methods of analyzing gene expression patterns.
PCT/US2000/031097 1999-11-16 2000-11-14 METHODS OF PREPARING cRNA WO2001036680A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU16018/01A AU1601801A (en) 1999-11-16 2000-11-14 Methods of preparing crna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16566899P 1999-11-16 1999-11-16
US60/165,668 1999-11-16

Publications (2)

Publication Number Publication Date
WO2001036680A2 WO2001036680A2 (en) 2001-05-25
WO2001036680A3 true WO2001036680A3 (en) 2002-02-14

Family

ID=22599929

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/031097 WO2001036680A2 (en) 1999-11-16 2000-11-14 METHODS OF PREPARING cRNA

Country Status (2)

Country Link
AU (1) AU1601801A (en)
WO (1) WO2001036680A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10112851C1 (en) * 2001-03-16 2002-10-10 Gsf Forschungszentrum Umwelt Semi-allogeneic anti-tumor vaccine with HLA-haplo-identical antigen-presenting cells
WO2003093504A1 (en) * 2002-05-06 2003-11-13 Noxxon Pharma Ag Method for amplifying nucleic acids
US20040096829A1 (en) * 2002-11-14 2004-05-20 Allaire Normand E. Absolute quantitation of nucleic acids by RT-PCR
US8697854B2 (en) 2008-11-24 2014-04-15 Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt Gmbh High affinity T cell receptor and use thereof
GB201301857D0 (en) * 2013-02-01 2013-03-20 Selvi Ozan Method
US9255265B2 (en) * 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0628640A1 (en) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Simultaneous amplification of multiple targets
US5514545A (en) * 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5514545A (en) * 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
EP0628640A1 (en) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Simultaneous amplification of multiple targets

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
APTE AND SIEBERT: "Anchor-Ligated cDNA Libraries: A Technique for Generating a cDNA Library for the Immediate Cloning of the 5' Ends of mRNAs", BIOTECHNIQUES, vol. 15, no. 5, 1993, pages 890 - 893, XP002149618 *
EDWARDS ET AL.: "Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' end of mRNAs and for constructing cDNA libraries by in vitro amplification", NUCLEIC ACIDS RESEARCH, no. 19, 11 October 1991 (1991-10-11), pages 5227 - 5232, XP000257040 *
KIEVITS ET AL.: "NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection", JOURNAL OF VIROLOGICAL METHODS, vol. 35, December 1991 (1991-12-01), pages 273 - 286, XP000576430 *

Also Published As

Publication number Publication date
AU1601801A (en) 2001-05-30
WO2001036680A2 (en) 2001-05-25

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