WO2001042497A3 - Monitoring oligonucleotide binding processes using chemiluminescence quenching - Google Patents

Monitoring oligonucleotide binding processes using chemiluminescence quenching Download PDF

Info

Publication number
WO2001042497A3
WO2001042497A3 PCT/GB2000/004721 GB0004721W WO0142497A3 WO 2001042497 A3 WO2001042497 A3 WO 2001042497A3 GB 0004721 W GB0004721 W GB 0004721W WO 0142497 A3 WO0142497 A3 WO 0142497A3
Authority
WO
WIPO (PCT)
Prior art keywords
quencher
oligonucleotide binding
binding processes
monitoring oligonucleotide
chemiluminescence quenching
Prior art date
Application number
PCT/GB2000/004721
Other languages
French (fr)
Other versions
WO2001042497A2 (en
WO2001042497A9 (en
Inventor
Andrew James Rutter
Ian Weeks
Zhaoqiang Li
Keith Smith
Original Assignee
Molecular Light Tech Res Ltd
Andrew James Rutter
Ian Weeks
Zhaoqiang Li
Keith Smith
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Light Tech Res Ltd, Andrew James Rutter, Ian Weeks, Zhaoqiang Li, Keith Smith filed Critical Molecular Light Tech Res Ltd
Priority to DE60039253T priority Critical patent/DE60039253D1/en
Priority to US10/149,146 priority patent/US7169554B2/en
Priority to ES00985518T priority patent/ES2304993T3/en
Priority to CA2393575A priority patent/CA2393575C/en
Priority to EP00985518A priority patent/EP1242622B1/en
Priority to DK00985518T priority patent/DK1242622T3/en
Priority to JP2001544369A priority patent/JP4722363B2/en
Priority to AU21933/01A priority patent/AU782674B2/en
Publication of WO2001042497A2 publication Critical patent/WO2001042497A2/en
Publication of WO2001042497A3 publication Critical patent/WO2001042497A3/en
Publication of WO2001042497A9 publication Critical patent/WO2001042497A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

Oligonucleotide building processes are monitored by means of an oligonucleotide probe which in one embodiment is labelled at one end with a chemiluminescent label and at the other end with a quencher molecule. The conformation of the oligonucleotide probe changes according to whether the probe hybridises with a substantially complementary nucleic acid sequence. In the non-hybridised state the chemiluminescent label is sufficiently close proximity to the quencher that the chemiluminescent emission is substantially attenuated, but in the hybridised state the separation is such that there is little or no attenuation. Particular probes and emitter/quencher pairs are disclosed.
PCT/GB2000/004721 1999-12-10 2000-12-11 Monitoring oligonucleotide binding processes using chemiluminescence quenching WO2001042497A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DE60039253T DE60039253D1 (en) 1999-12-10 2000-12-11 MONITORING OF OLIGONUCLEOTIDE BINDING BY CHEMILUMINESCENCE DELETION
US10/149,146 US7169554B2 (en) 1999-12-10 2000-12-11 Monitoring oligonucleotide binding processes using chemiluminescence quenching
ES00985518T ES2304993T3 (en) 1999-12-10 2000-12-11 MONITORING OF OLIGONUCLEOTIDE UNION PROCEDURES USING THE EXTINCTION OF CHEMOLUMINISCENCE.
CA2393575A CA2393575C (en) 1999-12-10 2000-12-11 Monitoring oligonucleotide binding processes using chemiluminescence quenching
EP00985518A EP1242622B1 (en) 1999-12-10 2000-12-11 Monitoring oligonucleotide binding processes using chemiluminescence quenching
DK00985518T DK1242622T3 (en) 1999-12-10 2000-12-11 Monitoring of oligonucleotide binding processes using chemiluminescence quenching
JP2001544369A JP4722363B2 (en) 1999-12-10 2000-12-11 Monitoring the oligonucleotide binding process using chemiluminescence quenching
AU21933/01A AU782674B2 (en) 1999-12-10 2000-12-11 Monitoring oligonucleotide binding processes using chemiluminescence quenching

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9929148.6 1999-12-10
GB9929148A GB2359625B (en) 1999-12-10 1999-12-10 Monitoring oligonucleotide binding process using chemiluminescence quenching

Publications (3)

Publication Number Publication Date
WO2001042497A2 WO2001042497A2 (en) 2001-06-14
WO2001042497A3 true WO2001042497A3 (en) 2001-12-27
WO2001042497A9 WO2001042497A9 (en) 2002-06-06

Family

ID=10866027

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004721 WO2001042497A2 (en) 1999-12-10 2000-12-11 Monitoring oligonucleotide binding processes using chemiluminescence quenching

Country Status (11)

Country Link
US (1) US7169554B2 (en)
EP (1) EP1242622B1 (en)
JP (1) JP4722363B2 (en)
AT (1) ATE398682T1 (en)
AU (1) AU782674B2 (en)
CA (1) CA2393575C (en)
DE (1) DE60039253D1 (en)
DK (1) DK1242622T3 (en)
ES (1) ES2304993T3 (en)
GB (1) GB2359625B (en)
WO (1) WO2001042497A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2359625B (en) 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
AU2002345746A1 (en) * 2001-06-21 2003-01-08 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
US6892092B2 (en) * 2001-10-29 2005-05-10 Cardiac Pacemakers, Inc. Cardiac rhythm management system with noise detector utilizing a hysteresis providing threshold
GB0320235D0 (en) * 2003-08-29 2003-10-01 Molecular Light Tech Res Ltd Estimation of activity or inhibition of processes involved in nucleic acid modification using chemiluminescence quenching
AU2010276236B2 (en) 2009-07-21 2014-03-20 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
WO2019116234A1 (en) 2017-12-12 2019-06-20 Nestec S.A. Methods for determining microbiome ribosomal rna composition changes

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4277437A (en) * 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
EP0070685A2 (en) * 1981-07-17 1983-01-26 Amoco Corporation Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
EP0439036A2 (en) * 1990-01-25 1991-07-31 F. Hoffmann-La Roche Ag Energy transferring system
WO1997001763A1 (en) * 1995-06-29 1997-01-16 Chiron Diagnostics Corporation Non-separation specific binding chemiluminescent assay
WO1998053316A1 (en) * 1997-05-23 1998-11-26 Boehringer Mannheim Corporation Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5928862A (en) * 1986-01-10 1999-07-27 Amoco Corporation Competitive homogeneous assay
WO1999042615A1 (en) * 1998-02-18 1999-08-26 Dade Behring Inc. Methods for determining amounts of nucleic acids

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
DE69435060T2 (en) 1993-11-12 2009-01-02 Phri Properties, Inc. HYBRIDIZATION TEST FOR NUCLEIC ACID DETERRECTION, UNIVERSAL STRAIN STRUCTURES, METHODS AND REAGENT SETS
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
EP0709466B1 (en) * 1994-10-28 2006-09-27 Gen-Probe Incorporated Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences
DE19502788C1 (en) * 1995-01-28 1996-09-05 Metallgesellschaft Ag Method and device for discharging a hot gas mixture containing carbon monoxide
CA2219891C (en) * 1995-05-05 2002-01-29 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5691145A (en) * 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
JP3016759B2 (en) 1997-02-28 2000-03-06 スミスクライン・ビーチャム・コーポレイション Fluorescent energy transfer by competitive hybridization
WO1999011813A2 (en) 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6355421B1 (en) 1997-10-27 2002-03-12 Boston Probes, Inc. Methods, kits and compositions pertaining to PNA molecular beacons
DE19808884A1 (en) 1998-03-03 1999-09-16 November Ag Molekulare Medizin Apparatus and method for detecting chemical substances
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
WO2000001850A2 (en) * 1998-07-02 2000-01-13 Gen-Probe Incorporated Molecular torches
AU5882000A (en) 1999-06-22 2001-01-09 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6783948B1 (en) * 1999-07-30 2004-08-31 Bayer Corporation Chemiluminescent acridinium compounds and analogues thereof as substrates of hydrolytic enzymes
GB2359625B (en) 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4277437A (en) * 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
EP0070685A2 (en) * 1981-07-17 1983-01-26 Amoco Corporation Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5928862A (en) * 1986-01-10 1999-07-27 Amoco Corporation Competitive homogeneous assay
EP0439036A2 (en) * 1990-01-25 1991-07-31 F. Hoffmann-La Roche Ag Energy transferring system
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
WO1997001763A1 (en) * 1995-06-29 1997-01-16 Chiron Diagnostics Corporation Non-separation specific binding chemiluminescent assay
WO1998053316A1 (en) * 1997-05-23 1998-11-26 Boehringer Mannheim Corporation Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers
WO1999042615A1 (en) * 1998-02-18 1999-08-26 Dade Behring Inc. Methods for determining amounts of nucleic acids

Also Published As

Publication number Publication date
ES2304993T3 (en) 2008-11-01
EP1242622A2 (en) 2002-09-25
WO2001042497A2 (en) 2001-06-14
GB2359625A (en) 2001-08-29
GB2359625B (en) 2004-10-20
JP2003516160A (en) 2003-05-13
ATE398682T1 (en) 2008-07-15
DE60039253D1 (en) 2008-07-31
WO2001042497A9 (en) 2002-06-06
DK1242622T3 (en) 2008-09-08
US20030082713A1 (en) 2003-05-01
GB9929148D0 (en) 2000-02-02
CA2393575C (en) 2012-01-31
JP4722363B2 (en) 2011-07-13
CA2393575A1 (en) 2001-06-14
AU2193301A (en) 2001-06-18
EP1242622B1 (en) 2008-06-18
AU782674B2 (en) 2005-08-18
US7169554B2 (en) 2007-01-30

Similar Documents

Publication Publication Date Title
DK0699768T3 (en) Methods for quenching in solution of fluorescence-labeled oligonucleotide probes
WO1999037806A3 (en) Modified nucleic acid probes and uses thereof
EP2366803A3 (en) Compositions and methods for detecting west nile virus
WO2002061133A3 (en) Nucleic acid detection using structured probes
WO2001027326A3 (en) Template-dependent ligation with pna-dna chimeric probes
WO2001096612A3 (en) Method of identifying and quantifying specific fungi and bacteria
AU3901095A (en) Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
NZ516278A (en) Improved primers and methods for the detection and discrimination of nucleic acids
CA2400240A1 (en) Multiplex ligatable probe amplification
WO1999049082A3 (en) Modified nucleotides and methods useful for nucleic acid sequencing
WO1999014375A3 (en) Dna typing by mass spectrometry with polymorphic dna repeat markers
WO2000056927A3 (en) Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1998026093A3 (en) Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
WO2004099431A3 (en) Nucleic acid sequence detection using multiplexed oligonucleotide pcr
WO1999050448A3 (en) Nucleic acid detection method
WO2002095057A3 (en) Pairs of nucleic acid probes with interactive signaling moieties and nucleic acid probes with enhanced hybridization efficiency and specificity
WO2004059289A3 (en) Target-dependent transcription using deletion mutants of n4 rna polymerase
CA2448211A1 (en) Nucleic acid detection method
WO2004010101A3 (en) Detection of chemical ligation using fluorescence quenching leaving groups
WO2001042497A3 (en) Monitoring oligonucleotide binding processes using chemiluminescence quenching
EP1243659A4 (en) Method for detecting target nucleotide sequences
EP1686191A3 (en) Fluorometric method for monitoring and detecting nucleic acid amplification
WO2000061801A3 (en) METHOD FOR THE DETECTION AND/OR ANALYSIS, BY MEANS OF PRIMER EXTENSION TECHNIQUES, OF SINGLE NUCLEOTIDE POLYMORPHISMS IN RESTRICTION FRAGMENTS, IN PARTICULAR IN AMPLIFIED RESTRICTION FRAGMENTS GENERATED USING AFLP$m(3)
WO2005012548A3 (en) Self-hybridizing multiple target nucleic acid probes and methods of use
WO2002029085A3 (en) Nucleotide detection method

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2000985518

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 21933/01

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2393575

Country of ref document: CA

AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 18 AND 22, DESCRIPTION, ADDED

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 544369

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 10149146

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2000985518

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 21933/01

Country of ref document: AU

WWG Wipo information: grant in national office

Ref document number: 2000985518

Country of ref document: EP