WO2001057256A3 - Rolling circle amplification assay for nucleic acid analysis - Google Patents

Rolling circle amplification assay for nucleic acid analysis Download PDF

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Publication number
WO2001057256A3
WO2001057256A3 PCT/US2001/003439 US0103439W WO0157256A3 WO 2001057256 A3 WO2001057256 A3 WO 2001057256A3 US 0103439 W US0103439 W US 0103439W WO 0157256 A3 WO0157256 A3 WO 0157256A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
tandem
padlock
detection
probes
Prior art date
Application number
PCT/US2001/003439
Other languages
French (fr)
Other versions
WO2001057256A2 (en
Inventor
Melanie M Mahtani
Original Assignee
Molecular Dynamics Inc
Melanie M Mahtani
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Dynamics Inc, Melanie M Mahtani filed Critical Molecular Dynamics Inc
Priority to AU2001234760A priority Critical patent/AU2001234760A1/en
Priority to EP01906910A priority patent/EP1252334A2/en
Publication of WO2001057256A2 publication Critical patent/WO2001057256A2/en
Publication of WO2001057256A3 publication Critical patent/WO2001057256A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Abstract

Method and reagents for analysis of nucleic acid sequences is disclosed. In this method a plurality of padlock probes is provided. Each padlock probe may hybridize to a locus on a target nucleic acid under hybridization conditions. If a targeted variant is present at the locus, the padlock probe may be ligated to form an amplification target circle. The amplification target circle acts as a template for production of tandem-sequence DNA. The tandem-sequence DNA may then be digested into non-tandem detection fragments which are subsequently separated and detected. The plurality of padlock probes are designed such that ligation of the probes, amplification of the target circle, and digestion of the tandem-sequence DNA subsequently produced, and detection may all be effected with the same set of reagents. Each probe targets a unique locus variant on the target nucleic acid sequence and produces a detection fragment that may be distinguished from detection fragments produced from other padlock probe in the plurality of padlock probes by using a fragment analysis detector.
PCT/US2001/003439 2000-02-04 2001-02-02 Rolling circle amplification assay for nucleic acid analysis WO2001057256A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001234760A AU2001234760A1 (en) 2000-02-04 2001-02-02 Rolling circle amplification assay for nucleic acid analysis
EP01906910A EP1252334A2 (en) 2000-02-04 2001-02-02 Rolling circle amplification assay for nucleic acid analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/498,585 2000-02-04
US09/498,585 US6221603B1 (en) 2000-02-04 2000-02-04 Rolling circle amplification assay for nucleic acid analysis

Publications (2)

Publication Number Publication Date
WO2001057256A2 WO2001057256A2 (en) 2001-08-09
WO2001057256A3 true WO2001057256A3 (en) 2002-07-25

Family

ID=23981667

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/003439 WO2001057256A2 (en) 2000-02-04 2001-02-02 Rolling circle amplification assay for nucleic acid analysis

Country Status (4)

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US (1) US6221603B1 (en)
EP (1) EP1252334A2 (en)
AU (1) AU2001234760A1 (en)
WO (1) WO2001057256A2 (en)

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US6221603B1 (en) 2001-04-24
WO2001057256A2 (en) 2001-08-09
EP1252334A2 (en) 2002-10-30
AU2001234760A1 (en) 2001-08-14

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