WO2001062982A3 - Methods for multi-stage solid phase amplification of nucleic acids - Google Patents

Methods for multi-stage solid phase amplification of nucleic acids Download PDF

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Publication number
WO2001062982A3
WO2001062982A3 PCT/US2001/005940 US0105940W WO0162982A3 WO 2001062982 A3 WO2001062982 A3 WO 2001062982A3 US 0105940 W US0105940 W US 0105940W WO 0162982 A3 WO0162982 A3 WO 0162982A3
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WO
WIPO (PCT)
Prior art keywords
amplification
methods
nucleic acids
solid phase
double
Prior art date
Application number
PCT/US2001/005940
Other languages
French (fr)
Other versions
WO2001062982A2 (en
Inventor
Thomas R Gingeras
T Christian Boles
Stephen P A Fodor
Christopher P Adams
Original Assignee
Mosaic Technologies Inc
Affymetrix Inc
Thomas R Gingeras
T Christian Boles
Stephen P A Fodor
Christopher P Adams
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies Inc, Affymetrix Inc, Thomas R Gingeras, T Christian Boles, Stephen P A Fodor, Christopher P Adams filed Critical Mosaic Technologies Inc
Priority to AU2001241723A priority Critical patent/AU2001241723A1/en
Publication of WO2001062982A2 publication Critical patent/WO2001062982A2/en
Publication of WO2001062982A3 publication Critical patent/WO2001062982A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/107—Chemical cleaving agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155—Particles of a defined size, e.g. nanoparticles

Abstract

The present invention discloses multi-stage bridge amplification methods which employs a double-stranded amplification product to initiate a subsequent stage of amplification. This double-stranded amplification product is formed during an amplification reaction and can be used to establish an iterative process. Using this double-stranded amplification product, the efficiency of bridge amplification increases significantly as a result of multiple stages of bridge amplification and permits, for example, the detection of a target molecule using a non-radioactive label (such as fluorescence).
PCT/US2001/005940 2000-02-25 2001-02-23 Methods for multi-stage solid phase amplification of nucleic acids WO2001062982A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001241723A AU2001241723A1 (en) 2000-02-25 2001-02-23 Methods for multi-stage solid phase amplification of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51330000A 2000-02-25 2000-02-25
US09/513,300 2000-02-25

Publications (2)

Publication Number Publication Date
WO2001062982A2 WO2001062982A2 (en) 2001-08-30
WO2001062982A3 true WO2001062982A3 (en) 2002-09-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/005940 WO2001062982A2 (en) 2000-02-25 2001-02-23 Methods for multi-stage solid phase amplification of nucleic acids

Country Status (2)

Country Link
AU (1) AU2001241723A1 (en)
WO (1) WO2001062982A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US9228228B2 (en) 2006-10-27 2016-01-05 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US9238834B2 (en) 2007-11-29 2016-01-19 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9334490B2 (en) 2006-11-09 2016-05-10 Complete Genomics, Inc. Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
US9476054B2 (en) 2005-06-15 2016-10-25 Complete Genomics, Inc. Two-adaptor library for high-throughput sequencing on DNA arrays

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US20050079497A1 (en) * 2001-10-27 2005-04-14 Sigma-Aldrich Co. Multiple oligonucleotides per gene for use in gene arrays
CA2930400A1 (en) 2003-02-26 2004-09-10 Callida Genomics, Inc. Apparatus with pixel alignment system for analyzing nucleic acid
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
GB0402895D0 (en) * 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
EP3492602A1 (en) 2005-06-15 2019-06-05 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
JP2009178159A (en) 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd Method of nucleic acid sequence detection and nucleic acid sequence detection substrate
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
JP5457222B2 (en) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー Miniaturized high-throughput nucleic acid analysis
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CA2814049C (en) 2010-10-08 2021-07-13 President And Fellows Of Harvard College High-throughput single cell barcoding
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
GB2495909A (en) * 2011-10-19 2013-05-01 Genome Analysis Ct Arrays comprising immobilized primer pair spots for amplifying target nucleic acids
US8778849B2 (en) 2011-10-28 2014-07-15 Illumina, Inc. Microarray fabrication system and method
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
PL3017065T3 (en) 2013-07-01 2019-03-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
EP3084523B1 (en) 2013-12-19 2019-07-03 Illumina, Inc. Substrates comprising nano-patterning surfaces and methods of preparing thereof
AU2017267653B2 (en) 2016-05-18 2021-05-13 Illumina, Inc. Self assembled patterning using patterned Hydrophobic surfaces
CN109688817B (en) 2016-10-03 2022-02-22 Illumina公司 Fluorescence detection and analysis method of amine and hydrazine
EP4209597A1 (en) 2017-08-01 2023-07-12 MGI Tech Co., Ltd. Nucleic acid sequencing method
EP3995590A1 (en) 2017-10-11 2022-05-11 MGI Tech Co., Ltd. Method for improving loading and stability of nucleic acid
WO2022021163A1 (en) 2020-07-29 2022-02-03 深圳华大智造科技股份有限公司 Method for loading nucleic acid molecule on solid support

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
WO1998036094A1 (en) * 1997-02-14 1998-08-20 Mosaic Technologies Method and apparatus for performing amplification of nucleic acid on supports
WO1998044151A1 (en) * 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
WO2000075374A1 (en) * 1999-06-04 2000-12-14 Mosaic Technologies Solid phase methods for amplifying multiple nucleic acids

Patent Citations (5)

* Cited by examiner, † Cited by third party
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US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
WO1998036094A1 (en) * 1997-02-14 1998-08-20 Mosaic Technologies Method and apparatus for performing amplification of nucleic acid on supports
WO1998044151A1 (en) * 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
WO2000075374A1 (en) * 1999-06-04 2000-12-14 Mosaic Technologies Solid phase methods for amplifying multiple nucleic acids

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9476054B2 (en) 2005-06-15 2016-10-25 Complete Genomics, Inc. Two-adaptor library for high-throughput sequencing on DNA arrays
US9228228B2 (en) 2006-10-27 2016-01-05 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US9334490B2 (en) 2006-11-09 2016-05-10 Complete Genomics, Inc. Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US9238834B2 (en) 2007-11-29 2016-01-19 Complete Genomics, Inc. Efficient shotgun sequencing methods

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Publication number Publication date
WO2001062982A2 (en) 2001-08-30
AU2001241723A1 (en) 2001-09-03

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