WO2001070946A3 - Methods for in vivo diversification of single genes - Google Patents

Methods for in vivo diversification of single genes Download PDF

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Publication number
WO2001070946A3
WO2001070946A3 PCT/US2001/009166 US0109166W WO0170946A3 WO 2001070946 A3 WO2001070946 A3 WO 2001070946A3 US 0109166 W US0109166 W US 0109166W WO 0170946 A3 WO0170946 A3 WO 0170946A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
methods
diversification
fungus
compositions
Prior art date
Application number
PCT/US2001/009166
Other languages
French (fr)
Other versions
WO2001070946A2 (en
Inventor
Edward B Cambareri
Eli E Kato
Original Assignee
Neugenesis Corp
Edward B Cambareri
Eli E Kato
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neugenesis Corp, Edward B Cambareri, Eli E Kato filed Critical Neugenesis Corp
Priority to AU2001247676A priority Critical patent/AU2001247676A1/en
Priority to US10/239,288 priority patent/US7323299B2/en
Priority to EP01920645A priority patent/EP1272626A2/en
Publication of WO2001070946A2 publication Critical patent/WO2001070946A2/en
Publication of WO2001070946A3 publication Critical patent/WO2001070946A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Abstract

Methods and compositions are provided for the efficient in vivo diversification of gene-products in filamentous fungi, starting from (but not limited to) two or more copies of a single gene consituent. The diversification involve use of the Repeat-Induced Point mutation (RIP) process in N. crassa, and other fungi that have analogous mutational processes. The invention takes advantage of the induction of G:C to A:T transition mutations specific to duplicated DNA sequences during the premeiotic dikaryion phase of the life cycle of the fungus. The methods and compositions of the invention can be utilitzed to generate diversity in target genes, and are proposed for the purpose of altering and generating novel forms and activities of gene-products thus encoded. Duplicated genes may be introduced into the organism and are present either in tandem, or at separate ectopic locations within the genome of the fungus. After crossing the resulting transformant(s), sexual progeny can then be selected which contain the mutated gene, and subsequently screened for a desired product.
PCT/US2001/009166 2000-03-21 2001-03-20 Methods for in vivo diversification of single genes WO2001070946A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2001247676A AU2001247676A1 (en) 2000-03-21 2001-03-20 Methods for in vivo diversification of single genes
US10/239,288 US7323299B2 (en) 2000-03-21 2001-03-20 Methods for in vivo diversification of single genes
EP01920645A EP1272626A2 (en) 2000-03-21 2001-03-20 Methods for in vivo diversification of single genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19093900P 2000-03-21 2000-03-21
US60/190,939 2000-03-21

Publications (2)

Publication Number Publication Date
WO2001070946A2 WO2001070946A2 (en) 2001-09-27
WO2001070946A3 true WO2001070946A3 (en) 2002-07-18

Family

ID=22703423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009166 WO2001070946A2 (en) 2000-03-21 2001-03-20 Methods for in vivo diversification of single genes

Country Status (4)

Country Link
US (1) US7323299B2 (en)
EP (1) EP1272626A2 (en)
AU (1) AU2001247676A1 (en)
WO (1) WO2001070946A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009095742A1 (en) 2008-01-31 2009-08-06 Cellectis New i-crei derived single-chain meganuclease and uses thereof
EP2559759A1 (en) 2003-01-28 2013-02-20 Cellectis Custom-made meganuclease and use thereof
US8032368B2 (en) * 2005-07-11 2011-10-04 Lg Electronics Inc. Apparatus and method of encoding and decoding audio signals using hierarchical block swithcing and linear prediction coding
EP2531609A1 (en) 2010-02-02 2012-12-12 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for the diagnosis and therapy of retinitis pigmentosa
US11845928B2 (en) 2015-05-04 2023-12-19 Tsinghua University Methods and kits for fragmenting DNA
WO2019234750A1 (en) 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of regenerating and transforming cannabis
WO2019234754A1 (en) 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Nucleic acid constructs and methods of using same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999027072A1 (en) * 1997-11-24 1999-06-03 Flinders Technologies Pty. Ltd. Reagents and methods for diversification of dna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999027072A1 (en) * 1997-11-24 1999-06-03 Flinders Technologies Pty. Ltd. Reagents and methods for diversification of dna

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BARBATO CHRISTIAN ET AL: "Mild RIP-an alternative method for in vivo mutagenesis of the albino-3-gene in Neurospora crassa.", MOLECULAR & GENERAL GENETICS, vol. 252, no. 4, 1996, pages 353 - 361, XP008001404, ISSN: 0026-8925 *
FOSS E J ET AL: "SPECIFICITY OF REPEAT-INDUCED POINT MUTATION RIP IN NEUROSPORA: SENSITIVITY OF NON-NEUROSPORA SEQUENCES, A NATURAL DIVERGED TANDEM DUPLICATION, AND UNIQUE DNA ADJACENT TO A DUPLICATED REGION", GENETICS, vol. 127, no. 4, 1991, pages 711 - 717, XP008001433, ISSN: 0016-6731 *

Also Published As

Publication number Publication date
US20030215933A1 (en) 2003-11-20
EP1272626A2 (en) 2003-01-08
AU2001247676A1 (en) 2001-10-03
WO2001070946A2 (en) 2001-09-27
US7323299B2 (en) 2008-01-29

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