WO2001073134A3 - Gene profiling arrays - Google Patents
Gene profiling arrays Download PDFInfo
- Publication number
- WO2001073134A3 WO2001073134A3 PCT/US2001/009993 US0109993W WO0173134A3 WO 2001073134 A3 WO2001073134 A3 WO 2001073134A3 US 0109993 W US0109993 W US 0109993W WO 0173134 A3 WO0173134 A3 WO 0173134A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mixtures
- specimens
- nucleic acid
- arrays
- cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Ordered arrays of mixtures of nucleic acid molecules are provided, which mixtures reflect the expression profile of one or more specimens, such as different cells or tissues. In particular embodiments, complete mRNA mixtures from specimens are separately arrayed on a substrate. Specimens from which such mixtures of nucleic acid molecules are produced can be taken from any source, including animal, plant and/or microbial cells, and can be assembled in any collection desired. The collections can, for instance, include different cell types, different phenotypes, cells grown under different conditions, cells of different ages or developmental stages, and so forth. The nucleic acid arrays are provided in both macro- and microarray formats, and are suitable for gene profiling in which relative quantitative expression from a single source or multiple sources may be determined. Techniques are also disclosed for producing high-fidelity, amplified mixtures of nucleic acid molecules using a combination of anti-sense RNA amplification and template-switching synthesis. Amplified mixtures produced using this method can, for instance, be applied to the disclosed arrays. The disclosed arrays allow high throughput analysis of differential gene expression in a specimen (such as a tumor) or a variety of specimens (such as a variety of tumors), and are suitable for automated preparation and analysis.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2001251069A AU2001251069A1 (en) | 2000-03-28 | 2001-03-28 | Gene profiling arrays |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US19270000P | 2000-03-28 | 2000-03-28 | |
US60/192,700 | 2000-03-28 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2001073134A2 WO2001073134A2 (en) | 2001-10-04 |
WO2001073134A3 true WO2001073134A3 (en) | 2003-01-16 |
Family
ID=22710712
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/009993 WO2001073134A2 (en) | 2000-03-28 | 2001-03-28 | Gene profiling arrays |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2001251069A1 (en) |
WO (1) | WO2001073134A2 (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7771934B2 (en) | 2000-12-13 | 2010-08-10 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7771946B2 (en) | 2001-03-09 | 2010-08-10 | Nugen Technologies, Inc. | Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US8465950B2 (en) | 2003-04-14 | 2013-06-18 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60141087D1 (en) * | 2000-06-26 | 2010-03-04 | Nugen Technologies Inc | METHODS AND COMPOSITIONS FOR TRANSCRIPTION-REPRODUCTIVE MACHINING OF NUCLEIC ACIDS |
US6794141B2 (en) | 2000-12-22 | 2004-09-21 | Arcturus Bioscience, Inc. | Nucleic acid amplification |
TWI335938B (en) * | 2001-08-15 | 2011-01-11 | Rna replication and amplification | |
AU2003220249A1 (en) * | 2002-03-15 | 2003-09-29 | Arcturus Bioscience, Inc. | Improved nucleic acid amplification |
EP1371726A1 (en) * | 2002-06-14 | 2003-12-17 | Rijksuniversiteit Groningen | Method for amplifying RNA |
DE10393431T5 (en) * | 2002-10-01 | 2005-11-17 | Nimblegen Systems, Inc., Madison | Microarrays with multiple oligonucleotides in single array features |
GB0319332D0 (en) * | 2003-08-16 | 2003-09-17 | Astrazeneca Ab | Amplification |
NL1026335C2 (en) * | 2004-06-04 | 2005-12-06 | Univ Delft Tech | Method for making a double-stranded polyribonucleotide sequence with overhanging end, as well as a method for forming a double-stranded polynucleotide construct and an application. |
EP2379748A4 (en) | 2008-12-23 | 2012-08-29 | Illumina Inc | Multibase delivery for long reads in sequencing by synthesis protocols |
EP3141613A1 (en) | 2015-09-11 | 2017-03-15 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Detection of quality of surface water |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997024455A2 (en) * | 1996-01-03 | 1997-07-10 | Clontech Laboratories, Inc. | METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING |
WO1998055502A1 (en) * | 1997-06-04 | 1998-12-10 | Smithkline Beecham Corporation | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
WO1999025873A1 (en) * | 1997-11-19 | 1999-05-27 | Incyte Pharmaceuticals, Inc. | METHOD FOR UNBIASED mRNA AMPLIFICATION |
-
2001
- 2001-03-28 AU AU2001251069A patent/AU2001251069A1/en not_active Abandoned
- 2001-03-28 WO PCT/US2001/009993 patent/WO2001073134A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997024455A2 (en) * | 1996-01-03 | 1997-07-10 | Clontech Laboratories, Inc. | METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING |
WO1998055502A1 (en) * | 1997-06-04 | 1998-12-10 | Smithkline Beecham Corporation | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
WO1999025873A1 (en) * | 1997-11-19 | 1999-05-27 | Incyte Pharmaceuticals, Inc. | METHOD FOR UNBIASED mRNA AMPLIFICATION |
Non-Patent Citations (4)
Title |
---|
DUGGAN D J ET AL: "EXPRESSION PROFILING USING CDNA MICROARRAYS", NATURE GENETICS, NEW YORK, NY, US, vol. 21, no. SUPPL, January 1999 (1999-01-01), pages 10 - 14, XP000865980, ISSN: 1061-4036 * |
SCHMIDT W.M. ET AL.,: "Capselect:a high sensitive method for 5'cap-dependent enrichment of full-length cDNA in PCR-mediate analysis of mRNAs", NUCLEIC ACID RESEARCH, vol. 27, no. 12, - 1999, pages e31, XP002211678 * |
WANG ENA ET AL.,: "high-fidelity mRNA amplification for gene profiling", NATURE BIOTECHNOLOGY, vol. 18, - April 2000 (2000-04-01), pages 457 - 459, XP002211679 * |
ZHAO N ET AL: "High-density cDNA filter analysis: a novel approach for large-scale, quantitative analysis of gene expression", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 156, no. 2, 1995, pages 207 - 213, XP004042356, ISSN: 0378-1119 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US7771934B2 (en) | 2000-12-13 | 2010-08-10 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US8334116B2 (en) | 2000-12-13 | 2012-12-18 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7771946B2 (en) | 2001-03-09 | 2010-08-10 | Nugen Technologies, Inc. | Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences |
US8071311B2 (en) | 2001-03-09 | 2011-12-06 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US9181582B2 (en) | 2001-03-09 | 2015-11-10 | Nugen Technologies, Inc. | Compositions for amplification of RNA sequences using composite primers |
US8465950B2 (en) | 2003-04-14 | 2013-06-18 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US9175325B2 (en) | 2003-04-14 | 2015-11-03 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8852867B2 (en) | 2005-09-07 | 2014-10-07 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
Also Published As
Publication number | Publication date |
---|---|
WO2001073134A2 (en) | 2001-10-04 |
AU2001251069A1 (en) | 2001-10-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2001073134A3 (en) | Gene profiling arrays | |
CN103397082B (en) | The random array DNA analysis carried out by hybridization | |
EP1848827B1 (en) | Methods and compositions for tagging and identifying polynucleotides | |
US20200370105A1 (en) | Methods for performing spatial profiling of biological molecules | |
DE69534389T2 (en) | Solid phase carriers equipped with PNA and amidate labels | |
CN105696088B (en) | A kind of double digestion simplifies genome two generations sequencing library construction method and matched reagent box | |
CN109562142A (en) | Use the DNA synthesis order-checking of nucleotide analog and Raman detection | |
Ivanov et al. | Galanthamine and related alkaloids production by Leucojum aestivum L. shoot culture using a temporary immersion technology | |
IL153504A0 (en) | Methods and compositions for amplification of rna sequences | |
CN102257162A (en) | Sequence preserved DNA conversion | |
WO1999051774A3 (en) | A method for obtaining a plant with a genetic lesion in a gene sequence | |
CN104254620A (en) | Method for amplifying nucleic acid and method for detecting amplified nucleic acid | |
EP1748085A1 (en) | Preparing RNA from a wax-embedded tissue specimen | |
Kawasaki | Microarrays and the gene expression profile of a single cell | |
CN109477137A (en) | Use the polynucleotides enrichment and amplification of ARGONAUTE system | |
Chaudhary et al. | Microbial profiles of rhizosphere and bulk soil microbial communities of biofuel crops switchgrass (Panicum virgatum L.) and jatropha (Jatropha curcas L.) | |
CN109563543A (en) | The method for generating nucleic acid library | |
Hamashima et al. | DNA sequencing method including unnatural bases for DNA aptamer generation by genetic alphabet expansion | |
CN112654699A (en) | Lipid-modified oligonucleotides and methods of use thereof | |
Såstad et al. | The narrow endemic Sphagnum troendelagicum is an allopolyploid derivative of the widespread S. balticum and S. tenellum | |
CN105899525A (en) | Efficient method for establishing induced pluripotent stem cells | |
CN107893067A (en) | The method based on solid phase carrier film enrichment target nucleic acid for high-flux sequence | |
CN107250381A (en) | The normalized iterative bar code of DNA set and sequencing | |
WO2005079531A3 (en) | Method and system for eluting cells | |
KR20220066839A (en) | Composition for detecting target nucleic acid and method for detecting using the same |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
122 | Ep: pct application non-entry in european phase | ||
NENP | Non-entry into the national phase |
Ref country code: JP |