WO2001088087A3 - Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation - Google Patents

Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation Download PDF

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Publication number
WO2001088087A3
WO2001088087A3 PCT/US2001/014822 US0114822W WO0188087A3 WO 2001088087 A3 WO2001088087 A3 WO 2001088087A3 US 0114822 W US0114822 W US 0114822W WO 0188087 A3 WO0188087 A3 WO 0188087A3
Authority
WO
WIPO (PCT)
Prior art keywords
embryos
microfluidic
embryo
channel
opportunity
Prior art date
Application number
PCT/US2001/014822
Other languages
French (fr)
Other versions
WO2001088087A2 (en
Inventor
David J Beebe
Ian K Glasgow
Matthew B Wheeler
Henry Zeringue
Original Assignee
Univ Illinois
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/570,483 external-priority patent/US6695765B1/en
Priority to CA002408616A priority Critical patent/CA2408616C/en
Priority to EP01933192A priority patent/EP1280465A4/en
Priority to AU5963501A priority patent/AU5963501A/en
Priority to MXPA02011150A priority patent/MXPA02011150A/en
Priority to AU2001259635A priority patent/AU2001259635B2/en
Application filed by Univ Illinois filed Critical Univ Illinois
Priority to JP2001585296A priority patent/JP2003533221A/en
Priority to KR1020027015046A priority patent/KR20030009482A/en
Priority to BR0110768-2A priority patent/BR0110768A/en
Priority to IL152650A priority patent/IL152650A/en
Publication of WO2001088087A2 publication Critical patent/WO2001088087A2/en
Publication of WO2001088087A3 publication Critical patent/WO2001088087A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Abstract

Microfluidic embryo scaled channels (14) for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners. Fluid flow is used to move and position embryos within microfluidic channels and channel geometrics may be used to place embryos at specific locations. Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evalutions at forces slightly below which damage to embryos is known to occur. Measurement of the distance and/or which embryos roll in a same pressure gradient microfluidic channel provides information, with healthy embryos traveling slower or a shorter distance as they demonstrate more stiction to channel walls.
PCT/US2001/014822 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation WO2001088087A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
IL152650A IL152650A (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
EP01933192A EP1280465A4 (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
AU5963501A AU5963501A (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
MXPA02011150A MXPA02011150A (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation.
AU2001259635A AU2001259635B2 (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
CA002408616A CA2408616C (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
JP2001585296A JP2003533221A (en) 2000-05-12 2001-05-08 Handling, analysis and biological evaluation of microchannel embryos and / or oocytes
KR1020027015046A KR20030009482A (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
BR0110768-2A BR0110768A (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and / or oocyte handling, analysis and biological evaluation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/570,483 2000-05-12
US09/570,483 US6695765B1 (en) 1999-04-08 2000-05-12 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Publications (2)

Publication Number Publication Date
WO2001088087A2 WO2001088087A2 (en) 2001-11-22
WO2001088087A3 true WO2001088087A3 (en) 2002-03-28

Family

ID=24279818

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/014822 WO2001088087A2 (en) 2000-05-12 2001-05-08 Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation

Country Status (11)

Country Link
EP (1) EP1280465A4 (en)
JP (1) JP2003533221A (en)
KR (1) KR20030009482A (en)
CN (1) CN1441652A (en)
AU (2) AU2001259635B2 (en)
BR (1) BR0110768A (en)
CA (1) CA2408616C (en)
IL (1) IL152650A (en)
MX (1) MXPA02011150A (en)
RU (1) RU2002130577A (en)
WO (1) WO2001088087A2 (en)

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WO2003085379A2 (en) * 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
WO2004101743A2 (en) * 2003-05-06 2004-11-25 Bellbrook Labs, Llc Three dimensional cell cultures in a microscale fluid handling system
JP2006527028A (en) * 2003-06-06 2006-11-30 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン Integrated device for extraction and insemination of microfluidic sperm
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP1609850A1 (en) 2004-06-24 2005-12-28 Biovir v/Jacob Mollenbach Culture dish for culturing biological cells
JP2006055072A (en) * 2004-08-20 2006-03-02 Fujitsu Ltd Apparatus and method for conveying control
CN101142309A (en) * 2005-03-18 2008-03-12 艾菲克特细胞研究所股份有限公司 Cell observation aiding instrument and method of cell observation therewith
GB0508983D0 (en) * 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
US20070090166A1 (en) * 2005-10-18 2007-04-26 Shuichi Takayama Microfluidic cell culture device
US20070084706A1 (en) * 2005-10-18 2007-04-19 Shuichi Takayama Microfluidic cell culture device and method for using same
CN101835886A (en) * 2007-08-24 2010-09-15 智能生物系统有限公司 Mesoscale bioreactor platform for perfusion
TW200920841A (en) * 2007-09-25 2009-05-16 Cytyc Corp Microfluidic apparatus for manipulating imaging and analyzing cells of a cytological specimen
KR100891487B1 (en) * 2007-11-01 2009-04-01 한국과학기술원 Enucleation system of oocyte or ovum which based on microfluid mechanics
WO2009079474A1 (en) * 2007-12-14 2009-06-25 The University Of North Carolina At Chapel Hill Methods, systems, and computer readable media for facilitating automation of blastocyst microinjection
CN102112593A (en) * 2008-08-01 2011-06-29 智能生物系统公司 A chamber of a bioreactor platform
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
GB0821636D0 (en) * 2008-11-26 2008-12-31 Ucl Business Plc Device
US20120196358A1 (en) * 2009-06-01 2012-08-02 Fred Burbank Device for removing cumulus from oocytes
US8621943B2 (en) * 2009-09-30 2014-01-07 Weyerhaeuser Nr Company Method of singulating embryos
EP2316565A1 (en) * 2009-10-26 2011-05-04 Fei Company A micro-reactor for observing particles in a fluid
CN101947124B (en) 2010-06-25 2012-07-04 博奥生物有限公司 Integrated microfluidic chip device and using method thereof
WO2012047678A2 (en) * 2010-09-27 2012-04-12 Auxogyn, Inc. Apparatus, method, and system for the automated imaging and evaluation of embryos, oocytes, and stem cells
WO2013158658A1 (en) * 2012-04-16 2013-10-24 Cornell University Automated intracytoplasmic sperm injection assisted fertilization system
US9757726B2 (en) 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
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JP2020510417A (en) 2017-02-17 2020-04-09 コニク インコーポレイテッド System for detection
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WO2019195620A1 (en) * 2018-04-04 2019-10-10 The General Hospital Corporation Microfluidic systems and methods to denude mammalian oocytes
US11654434B2 (en) 2018-06-24 2023-05-23 Nemametrix Inc. Microinjection chip, device, system and uses thereof for unicellular or multicellular organisms
FR3084121B1 (en) * 2018-07-17 2021-01-15 Skf Aerospace France MECHANICAL ASSEMBLY AND MECHANICAL DEVICE
CN111073817A (en) * 2018-10-22 2020-04-28 曾繁根 Integrated wafer and method for sperm sorting, ovum breeding, in vitro fertilization
CN111378556A (en) * 2018-12-29 2020-07-07 青岛华大智造普惠科技有限公司 Micro-fluidic chip and preparation method thereof, and preparation method of single-cell micro-droplets
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Also Published As

Publication number Publication date
CN1441652A (en) 2003-09-10
AU5963501A (en) 2001-11-26
KR20030009482A (en) 2003-01-29
CA2408616C (en) 2007-10-02
BR0110768A (en) 2003-03-11
EP1280465A2 (en) 2003-02-05
RU2002130577A (en) 2004-03-27
AU2001259635B2 (en) 2005-05-19
MXPA02011150A (en) 2004-08-19
IL152650A (en) 2006-12-31
CA2408616A1 (en) 2001-11-22
EP1280465A4 (en) 2004-10-20
IL152650A0 (en) 2003-06-24
JP2003533221A (en) 2003-11-11
WO2001088087A2 (en) 2001-11-22

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