WO2001094638A3 - Asynchronous primed pcr - Google Patents
Asynchronous primed pcr Download PDFInfo
- Publication number
- WO2001094638A3 WO2001094638A3 PCT/US2001/018464 US0118464W WO0194638A3 WO 2001094638 A3 WO2001094638 A3 WO 2001094638A3 US 0118464 W US0118464 W US 0118464W WO 0194638 A3 WO0194638 A3 WO 0194638A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- probe
- asynchronous
- reporter dye
- stranded
- annealed
- Prior art date
Links
- 239000000523 sample Substances 0.000 abstract 5
- 238000002844 melting Methods 0.000 abstract 4
- 230000008018 melting Effects 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 238000005382 thermal cycling Methods 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 239000003298 DNA probe Substances 0.000 abstract 1
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 238000000137 annealing Methods 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT01946152T ATE448325T1 (en) | 2000-06-06 | 2001-06-06 | ASYNCHRONOUS PCR PRIMING |
AU6823501A AU6823501A (en) | 2000-06-06 | 2001-06-06 | Asynchronous primed pcr |
EP01946152A EP1368489B1 (en) | 2000-06-06 | 2001-06-06 | Asynchronous primed pcr |
CA002412413A CA2412413A1 (en) | 2000-06-06 | 2001-06-06 | Asynchronous primed pcr |
DE60140487T DE60140487D1 (en) | 2000-06-06 | 2001-06-06 | Asynchrones pcr priming |
JP2002502178A JP2004509613A (en) | 2000-06-06 | 2001-06-06 | Asynchronous stimulus PCR |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20988300P | 2000-06-06 | 2000-06-06 | |
US60/209,883 | 2000-06-06 | ||
US09/875,211 | 2001-06-05 | ||
US09/875,211 US6887664B2 (en) | 2000-06-06 | 2001-06-05 | Asynchronous primed PCR |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2001094638A2 WO2001094638A2 (en) | 2001-12-13 |
WO2001094638A8 WO2001094638A8 (en) | 2002-04-11 |
WO2001094638A3 true WO2001094638A3 (en) | 2003-07-10 |
Family
ID=26904613
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/018464 WO2001094638A2 (en) | 2000-06-06 | 2001-06-06 | Asynchronous primed pcr |
Country Status (8)
Country | Link |
---|---|
US (2) | US6887664B2 (en) |
EP (3) | EP2256213A1 (en) |
JP (1) | JP2004509613A (en) |
AT (1) | ATE448325T1 (en) |
AU (1) | AU6823501A (en) |
CA (1) | CA2412413A1 (en) |
DE (1) | DE60140487D1 (en) |
WO (1) | WO2001094638A2 (en) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6887664B2 (en) * | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
JP4808365B2 (en) * | 2001-03-02 | 2011-11-02 | ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | PCR method |
US20030175749A1 (en) | 2001-12-08 | 2003-09-18 | Jong-Yoon Chun | Annealing control primer and its uses |
EP1942196B1 (en) | 2001-12-19 | 2012-05-30 | Brandeis University | Late-PCR |
US7198897B2 (en) * | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
JP2003199568A (en) * | 2002-01-10 | 2003-07-15 | Nichirei Corp | Method for improving efficiency of dna amplification reaction |
ES2527068T3 (en) | 2002-10-23 | 2015-01-19 | University Of Utah Research Foundation | Fusion analysis of amplicons with saturation dyes |
JP4280519B2 (en) * | 2003-03-07 | 2009-06-17 | アークレイ株式会社 | DNA amplification method and kit therefor |
JP2005000162A (en) * | 2003-05-19 | 2005-01-06 | Canon Inc | Pcr amplification method for nucleic acid, pcr primer set, pcr amplification product, and method for detecting the nucleic acid using the amplification method |
JP4322554B2 (en) * | 2003-05-19 | 2009-09-02 | 株式会社ニチレイフーズ | Method for improving efficiency of DNA amplification reaction |
US20040235032A1 (en) * | 2003-05-19 | 2004-11-25 | Canon Kabushiki Kaisha | PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method |
WO2005045073A1 (en) * | 2003-11-10 | 2005-05-19 | Seegene, Inc. | Method for amplifying unknown dna sequence adjacent to known sequence |
US7927797B2 (en) * | 2004-01-28 | 2011-04-19 | 454 Life Sciences Corporation | Nucleic acid amplification with continuous flow emulsion |
US7481997B1 (en) | 2004-02-12 | 2009-01-27 | Montana State University | Snow mountain virus genome sequence, virus-like particles and methods of use |
US9657347B2 (en) | 2004-04-20 | 2017-05-23 | University of Utah Research Foundation and BioFire Defense, LLC | Nucleic acid melting analysis with saturation dyes |
US7387887B2 (en) * | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
WO2006112818A2 (en) | 2005-04-14 | 2006-10-26 | Applera Corporation | 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes |
EP2813581B1 (en) * | 2005-01-06 | 2018-06-27 | Applied Biosystems, LLC | Use of polypeptides having nucleic acid binding activity in methods for fast nucleic acid amplification |
US20070015180A1 (en) * | 2005-05-02 | 2007-01-18 | Stratagene California | Oligonucleotide probe/primer compositions and methods for polynucleotide detection |
AU2006341607B2 (en) * | 2005-05-31 | 2011-03-17 | Applied Biosystems, Llc. | Multiplexed amplification of short nucleic acids |
DK2236616T3 (en) * | 2005-06-02 | 2014-01-13 | Advandx Inc | Peptide nucleic acid probes for analysis of microorganisms |
US20070087360A1 (en) * | 2005-06-20 | 2007-04-19 | Boyd Victoria L | Methods and compositions for detecting nucleotides |
US20070059713A1 (en) | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
EP1943348B1 (en) | 2005-10-03 | 2013-01-02 | Life Technologies Corporation | Compositions, methods, and kits for amplifying nucleic acids |
EP2759307B1 (en) | 2006-03-29 | 2016-11-09 | Merial Limited | Vaccine against Streptococci |
CA2651815A1 (en) | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
US7915014B2 (en) | 2006-07-17 | 2011-03-29 | Brandeis University | Specialized oligonucleotides and their use in nucleic acid amplification and detection |
WO2008072933A1 (en) * | 2006-12-15 | 2008-06-19 | Panagene Inc. | Peptide nucleic acids conjugated with multi -amine linkers and nucleic acid detecting device using the same |
KR101072900B1 (en) * | 2007-12-04 | 2011-10-17 | 주식회사 파나진 | Method for selective labeling and detection of target nucleic acids using immobilized peptide nucleic acid probes |
FI121379B (en) * | 2008-01-17 | 2010-10-29 | Mobidiag Oy | Process for the preparation of a single-stranded DNA by two-step symmetric PCR and reagent packaging for the process |
GB0806041D0 (en) | 2008-04-03 | 2008-05-14 | Genomica S A | Method for detection of herpesvirus in test sample |
US8993230B2 (en) * | 2008-12-04 | 2015-03-31 | Pacific Biosciences of Californ, Inc. | Asynchronous sequencing of biological polymers |
WO2010114599A1 (en) | 2009-04-01 | 2010-10-07 | Dx Terity Diagnostics Inc. | Chemical ligation dependent probe amplification (clpa) |
US9334531B2 (en) * | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US20110039304A1 (en) * | 2009-08-12 | 2011-02-17 | President And Fellows Of Harvard College | Methods to Generate Oligonucleotide Pools and Enrich Target Nucleic Acid Sequences |
EP2670865A4 (en) * | 2011-01-31 | 2014-09-10 | Primeradx Inc | Methods of nucleic acid quantification and detection using anomalous migration |
EP2710145B1 (en) | 2011-05-17 | 2015-12-09 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
EP2722399A1 (en) | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Method for preventing high molecular weight products during amplification |
US10942184B2 (en) | 2012-10-23 | 2021-03-09 | Caris Science, Inc. | Aptamers and uses thereof |
AU2015274660B2 (en) | 2014-06-10 | 2020-07-16 | Dxterity Diagnostics Incorporated | Devices and methods for collecting and stabilizing biological samples |
CN106536758A (en) * | 2014-07-25 | 2017-03-22 | 帕那金股份有限公司 | Use of multiple target nucleic acid detection method using clamping probe and detection probe |
PL3298162T3 (en) * | 2015-05-18 | 2020-07-27 | Saga Diagnostics Ab | Detection of target nucleic acid and variants |
US10443095B2 (en) | 2016-08-02 | 2019-10-15 | Roche Molecular Systems, Inc. | Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids |
CN108342489A (en) * | 2017-01-23 | 2018-07-31 | 公安部物证鉴定中心 | A kind of method and system carrying out Y-SNP partings to male individual |
US10513730B2 (en) * | 2017-11-22 | 2019-12-24 | Safeguard Biosystems Holdings Ltd. | Asymmetric PCR methods, primers and kits |
KR101919896B1 (en) * | 2018-05-03 | 2018-11-19 | 주식회사 유디피아 | Method for detecting multiple nucleic acid using a single fluorescence channel PCR machine |
WO2020046809A1 (en) * | 2018-08-27 | 2020-03-05 | The Regents Of The University Of California | Reporter nucleic acids for type v crispr-mediated detection |
KR20210026867A (en) * | 2019-09-02 | 2021-03-10 | 주식회사 시선바이오머티리얼스 | Novel peptide nucleic acid oligomer, thereof use as DNA methylation discrimination, and method for detecting DNA methylation using the same |
EP4087941A4 (en) * | 2020-01-10 | 2023-06-28 | Siemens Healthcare Diagnostics, Inc. | Compositions, kits, and methods for performing rapid polymerase chain reactions |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0640828A1 (en) * | 1993-08-27 | 1995-03-01 | F. Hoffmann-La Roche AG | Monitoring multiple reactions simultaneously and analyzing same |
US5972693A (en) * | 1995-10-24 | 1999-10-26 | Curagen Corporation | Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
EP0953379A1 (en) * | 1998-05-01 | 1999-11-03 | F. Hoffmann-La Roche Ag | Apparatus for simultaneously monitoring reactions taking place in a plurality of reaction vessels |
Family Cites Families (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US4973679A (en) | 1981-03-27 | 1990-11-27 | University Patents, Inc. | Process for oligonucleo tide synthesis using phosphormidite intermediates |
US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
US5151507A (en) | 1986-07-02 | 1992-09-29 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US4931223A (en) | 1986-07-24 | 1990-06-05 | Tropix, Inc. | Methods of using chemiluminescent 1,2-dioxetanes |
JPH02504672A (en) | 1986-12-15 | 1990-12-27 | ブリティッシュ テクノロジー グループ ユーエスエイ,インコーポレイテッド | Monomeric phthalocyanine reagent |
US5231191A (en) | 1987-12-24 | 1993-07-27 | Applied Biosystems, Inc. | Rhodamine phosphoramidite compounds |
US5047524A (en) | 1988-12-21 | 1991-09-10 | Applied Biosystems, Inc. | Automated system for polynucleotide synthesis and purification |
US5262530A (en) | 1988-12-21 | 1993-11-16 | Applied Biosystems, Inc. | Automated system for polynucleotide synthesis and purification |
US5141813A (en) | 1989-08-28 | 1992-08-25 | Clontech Laboratories, Inc. | Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis |
US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
US5654442A (en) | 1989-11-14 | 1997-08-05 | The Perkin-Elmer Corporation | 4,7-dichlorofluorescein dyes as molecular probes |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
DK51092D0 (en) | 1991-05-24 | 1992-04-15 | Ole Buchardt | OLIGONUCLEOTIDE ANALOGUE DESCRIBED BY PEN, MONOMERIC SYNTHONES AND PROCEDURES FOR PREPARING THEREOF, AND APPLICATIONS THEREOF |
DK0519338T3 (en) * | 1991-06-20 | 1996-10-28 | Hoffmann La Roche | Improved Methods for Nucleic Acid Amplification |
US5470705A (en) | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
WO1993020236A1 (en) | 1992-04-03 | 1993-10-14 | Applied Biosystems, Inc. | Probe composition and method |
US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
EP0760008A1 (en) | 1994-05-19 | 1997-03-05 | Dako A/S | Pna probes for detection of neisseria gonorrhoeae and chlamydia trachomatis |
US5801155A (en) | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
US5736626A (en) | 1996-01-29 | 1998-04-07 | The Perkin-Elmer Corporation | Solid support reagents for the direct synthesis of 3'-labeled polynucleotides |
US6020481A (en) | 1996-04-01 | 2000-02-01 | The Perkin-Elmer Corporation | Asymmetric benzoxanthene dyes |
EP0892808B1 (en) | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
US5847162A (en) | 1996-06-27 | 1998-12-08 | The Perkin Elmer Corporation | 4, 7-Dichlororhodamine dyes |
SE506700C2 (en) | 1996-05-31 | 1998-02-02 | Mikael Kubista | Probe and Methods for Analysis of Nucleic Acid |
US5821356A (en) | 1996-08-12 | 1998-10-13 | The Perkin Elmer Corporation | Propargylethoxyamino nucleotides |
DE19637339A1 (en) | 1996-09-13 | 1998-03-19 | Hoechst Ag | Methods for amplifying nucleic acids |
ES2192672T3 (en) | 1996-11-18 | 2003-10-16 | Takeshi Imanishi | NEW ANALOGS OF NUCLEOTIDES. |
US6110676A (en) | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
JP3756313B2 (en) | 1997-03-07 | 2006-03-15 | 武 今西 | Novel bicyclonucleosides and oligonucleotide analogues |
US5770716A (en) | 1997-04-10 | 1998-06-23 | The Perkin-Elmer Corporation | Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same |
IL135000A0 (en) | 1997-09-12 | 2001-05-20 | Exiqon As | Bi- and tri-cyclic nucleoside, nucleotide and oligonucleotide analogues |
US6008379A (en) | 1997-10-01 | 1999-12-28 | The Perkin-Elmer Corporation | Aromatic-substituted xanthene dyes |
US6485901B1 (en) | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
AU1366299A (en) | 1997-10-27 | 1999-05-17 | Boston Probes, Inc. | Methods, kits and compositions pertaining to pna molecular beacons |
US5936087A (en) | 1997-11-25 | 1999-08-10 | The Perkin-Elmer Corporation | Dibenzorhodamine dyes |
US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
US6361942B1 (en) | 1998-03-24 | 2002-03-26 | Boston Probes, Inc. | Method, kits and compositions pertaining to detection complexes |
US6117986A (en) | 1998-06-10 | 2000-09-12 | Intergen Company, L.P. | Pyrimidines linked to a quencher |
US6037130A (en) | 1998-07-28 | 2000-03-14 | The Public Health Institute Of The City Of New York, Inc. | Wavelength-shifting probes and primers and their use in assays and kits |
US6887664B2 (en) * | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
-
2001
- 2001-06-05 US US09/875,211 patent/US6887664B2/en not_active Expired - Lifetime
- 2001-06-06 CA CA002412413A patent/CA2412413A1/en not_active Abandoned
- 2001-06-06 AU AU6823501A patent/AU6823501A/en active Pending
- 2001-06-06 AT AT01946152T patent/ATE448325T1/en not_active IP Right Cessation
- 2001-06-06 WO PCT/US2001/018464 patent/WO2001094638A2/en active IP Right Grant
- 2001-06-06 EP EP09014084A patent/EP2256213A1/en not_active Withdrawn
- 2001-06-06 JP JP2002502178A patent/JP2004509613A/en active Pending
- 2001-06-06 DE DE60140487T patent/DE60140487D1/en not_active Expired - Lifetime
- 2001-06-06 EP EP10184140A patent/EP2316975A1/en not_active Withdrawn
- 2001-06-06 EP EP01946152A patent/EP1368489B1/en not_active Expired - Lifetime
-
2004
- 2004-06-09 US US10/865,683 patent/US20050130178A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0640828A1 (en) * | 1993-08-27 | 1995-03-01 | F. Hoffmann-La Roche AG | Monitoring multiple reactions simultaneously and analyzing same |
US5972693A (en) * | 1995-10-24 | 1999-10-26 | Curagen Corporation | Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
EP0953379A1 (en) * | 1998-05-01 | 1999-11-03 | F. Hoffmann-La Roche Ag | Apparatus for simultaneously monitoring reactions taking place in a plurality of reaction vessels |
Non-Patent Citations (1)
Title |
---|
AKIYAMA H ET AL: "An improved quantitative RT-PCR fluorescent method for analysis of gene transcripts in the STS-65 space shuttle experiment", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 47, no. 2, 27 June 1996 (1996-06-27), pages 325 - 333, XP004037009, ISSN: 0168-1656 * |
Also Published As
Publication number | Publication date |
---|---|
US6887664B2 (en) | 2005-05-03 |
EP1368489B1 (en) | 2009-11-11 |
US20050130178A1 (en) | 2005-06-16 |
EP2316975A1 (en) | 2011-05-04 |
CA2412413A1 (en) | 2001-12-13 |
WO2001094638A8 (en) | 2002-04-11 |
ATE448325T1 (en) | 2009-11-15 |
JP2004509613A (en) | 2004-04-02 |
US20030207266A1 (en) | 2003-11-06 |
AU6823501A (en) | 2001-12-17 |
WO2001094638A2 (en) | 2001-12-13 |
EP1368489A2 (en) | 2003-12-10 |
DE60140487D1 (en) | 2009-12-24 |
EP2256213A1 (en) | 2010-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2001094638A8 (en) | Asynchronous primed pcr | |
CA2532160A1 (en) | Methods of genotyping using differences in melting temperature | |
EP1866434B1 (en) | Isothermal nucleic acid amplification | |
CA2511128A1 (en) | Late-pcr | |
CA2658071C (en) | Specialized oligonucleotides and their use in nucleic acid amplification and detection | |
EP1716257B8 (en) | New primers and probes for the detection of parvovirus b19 | |
JP6166342B2 (en) | Polymerase chain reaction detection system using oligonucleotides containing phosphorothioate groups | |
CN104487596B (en) | Novel compositions, methods and kits for Polymerase Chain Reaction (PCR) | |
RU2012142160A (en) | DETECTION OF NUCLEIC-ACID TARGET SEQUENCES IN ANALYSIS WITH SPLITTING AND EXTENSION OF RTO | |
ATE489473T1 (en) | ANALYSIS OF MESSENGER RNA AND MICRO-RNA IN THE SAME REACTION APPROACH | |
ATE396280T1 (en) | DETECTION AND AMPLIFICATION OF RNA USING TARGETED DNA LIGATION BY RNA LIGASE | |
WO2007127992A3 (en) | Use of base-modified deoxynucleoside triphosphates | |
WO2004074447A2 (en) | Compositions and methods for multiplex analysis of polynucleotides | |
DE60108897D1 (en) | METHODS FOR EXTERNAL CONTROLS FOR NUCLEIC ACID AMPLIFICATION | |
JP2009511056A5 (en) | ||
US9914963B2 (en) | Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes | |
JP2015507931A5 (en) | ||
KR102324117B1 (en) | Detection of nucleic acids by strand invasion based amplification | |
JP2004526442A5 (en) | ||
EP3152324B1 (en) | Strand-invasion based dna amplification method | |
EP2373808B1 (en) | Enhanced taqman probe based amplification | |
WO2005047468A3 (en) | Improved methods for detecting and measuring specific nucleic acid sequences | |
CA2472970A1 (en) | New detection format for hot start real time polymerase chain reaction | |
KR20140123858A (en) | Polynucleotide and use thereof | |
ATE420208T1 (en) | IMPROVED COAMPLIFICATION OF NUCLEIC ACIDS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
AK | Designated states |
Kind code of ref document: C1 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: C1 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
CFP | Corrected version of a pamphlet front page | ||
CR1 | Correction of entry in section i |
Free format text: PAT. BUL. 50/2001 UNDER (30) REPLACE "NOT FURNISHED" BY "09/875211" |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2001946152 Country of ref document: EP Ref document number: 2412413 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2001268235 Country of ref document: AU |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 2001946152 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 2001268235 Country of ref document: AU |